TurboVap 96 User`s Manual
Contents
1. 21 iInstallation 23 specifications 23 41 VOMONG naaa FA REA GAA AGA KALAKI 10 W WISIN es serre ci Choo sisi 40 P N 128097 Rev 00 TurboVap 96 User s Manual Index 45 Biotage AB O Biotage Biotage AB 10430 Harris Oaks Blvd Suite C Charlotte NC 28269 TEL 1 704 654 4900 Toll Free 1 800 446 4752 FAX 1 704 654 4917 http www biotage com2. Biotage TurboVap 96 Concentration Workstation User s Manual CE Copyright 2010 Biotage AB All rights reserved P N 128097 Rev 00 Copyright Trademarks Content Preface 2 Preface This manual is published by Biotage AB 10430 Harris Oaks Blvd Suite C Charlotte NC 28269 U S A Copyright 2010 Biotage AB All rights reserved Reproduction by any means or in any form of this manual or the products it describes is prohibited Biotage and TurboVap are trademarks of Biotage Microsoft and Windows are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries All other trademarks and registered trademarks are the property of their respective holders The information in this manual may contain typographical errors or technical inaccuracies and is subject to change without notice Modifications may also be made to the product described in this manual at any time Proper Equipment Operation The TurboVap 96 Concentration Workstation is a microprocessor controlled instrument that provides simultaneous automated concentration of multiple samples unattended operation convenience and speed The TurboVap 96 can simultaneously process one or two 96 well microplates or deepwell plates It is the customer s responsibility to determine suitable methods for evaporation of their samples WARNINGS AA e The TurboVap 96 is NOT designed for in vitro testing or for use
3. 12 Power installing cord 24 required 21 specifications 40 switch location 16 TUNING ON 4 446 wa KG 64404 404 4 24 Power entry port 15 changing fuses 35 Power Up diagnostics on start up 24 Q Quick disconnect gas inlet 15 R Regulator knobs 13 Replacing the fuses 35 Restarting concentration 33 Routine maintenance 34 Biotage AB S Select button 18 Service panel 15 SEL DUUON sr sims sis 18 Shearing action 10 Site preparation 20 Solvent vapors 10 Space required 20 Specifications 40 Start Stop buttons 18 Starting concentration 31 Stopping concentration 33 T Temperature setting for position 1 28 setting for position 2 29 Temperature control specifications 41 Time range 40 Troubleshooting 37 concentration 37 front panel display 38 workstation not operational 39 TURING ON kawa eee id e 24 U Unpacking instructions 19 Up and Down arrow buttons 18 V Vapor removal 11 Ventilation SAatUreS 445 x44 000 tite rs 12 requirements 21 Venting hose
4. Beeper volume 15 C Capacity 40 Carbon trap recommendation 20 Changing the fuses 35 Cleaning control panel 34 exterior 34 Concentration completing 33 establishing conditions 25 DAUSING ss 33 restarting 33 starting 31 Control panel cleaning 34 Cover 13 maintenance 34 sensor features 11 Cycle readout during operation 32 Cycle Readout light 18 D Daily maintenance 34 Deepwell plates 14 specification 40 Diagnostics 24 Dimensions 40 Display cycle readout during operation 32 elapsed time display 32 Weiss rro PA hed eee 38 Cone ey ee re ee E AA 38 test on power up 24 troubleshooting 38 E Elapsed Time display during operation 32 P N 128097 Rev 00 TurboVap 96 User s Manual Index 43 WOM aser ba ee 17 resetting to zero 33 Environmental specifications 40 Evaporation time infinite 32 setting for position 1 27 Exhaust features 12 installing vent hose 23 maintenance 34 port location 15 requirements 21
5. the POWer CO Taasan end beet tenue een eas 24 URONG io LA ln 24 E OWer UW DIDI OM OSI CS ES NANO oe aoe NAA auto dai 24 Determining Optimal Concentration Conditions 25 Considerations when Selecting Plate Temperature u 2 2 2 X 25 Considerations When Setting the Gas Flow Rate 2 2 aa 26 DCF Ae OM a a AA Aa AA 27 a ne UNIES o dc An en 27 Setting the Gas Time Evaporation Time for Position 1 27 Setting the Plate Temperature for Position 1 28 Setting the Flow Rate for Position li NAA ANAN 28 Setting the Gas Time Evaporation Time for Position 2 29 Setting the Plate Temperature for Position 2 29 P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Table of Contents 9 Setting the Flow Rate for Position 2 30 Starting ihe ConcentalON ise io 31 Elapsed Time DISPARA 32 A o AA anne re een rer ee Te Oe E GAANO NAA manas 32 Pausmo the Concentraci n 33 ReStating the ConcentratlON SE AGA a NAA 33 COmpleting the COMCCNINATON een inerte N e ile 33 Mamtenance in ne ie 34 Dally MAIN enance sense A AA DA A Ada 34 Routine Maintenance sissen a DAA nh GA 34 Fee OAC ING ING USC AA 35 Long Leni StOLag AA 36 TFOUDIEeSNOO IM AN yearns ee een AA eee 37 Troubleshooting the Concentration 37 Troubleshooting the Front Panel Display 38 Workstation NOt Operational nn ANAN een diet 39 DHECHICATIONS a AA AA AA NAAN das 40 Lilo AA sane nn
6. ON the power switch located on the lower left side of the unit When the power is turned on the internal air temperature will heat up to the previously set plate temperature if it was set to below 37 C or it will heat up to 37 C if it is set above this value Power Up Diagnostics When the power is turned on both the Value and Units displays show 88 and all LEDs are lit for 1 5 seconds Confirm that all lights are working properly If you press the Select button while in this state the workstation enters diagnostics mode displaying the current firmware revision After 1 5 seconds initialization continues Also during this time raising and lowering the cover sounds the beeper to confirm the cover switch is functioning After initialization the workstation lights the Position 1 Gas Time light and displays the Gas Time previously set for Position 1 P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Determining Optimal Concentration Conditions 25 Determining Optimal Concentration Conditions You should determine the settings that will best concentrate your samples before operation You should determine the desired e Gas time evaporation time e Plate temperature e Flow rate Considerations when Selecting Plate Temperature The two heaters operate over a temperature range of 20 C ambient to 80 C 5 C The mild temperature range eliminates hot spots and improves sample recovery for more volatile co
7. Rev 00 TurboVap 96 User s Manual Biotage AB Hardware 14 TurboVap 96 Cover Open sue ahs Sw EE het AHE E ee DES A oo Gas Manifolds Microplate Plate Adapter Location Heater Figure 4 TurboVap 96 with Cover Open Gas Manifolds Each manifold consists of 96 individual gas nozzles which fit inside each microplate well Each nozzle supplies gas to concentrate the sample in one microplate well Microplates and deepwell plates Hold the samples for concentration Microplate Adapter Used with microplates so that when the cover is closed the manifold nozzles fit inside each microplate well Not used with deepwell plates Heater assembly Warms the samples during concentration to aid in evaporation P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Hardware 15 TurboVap 96 Rear Alarm Volume Service onc Panel 3 Quick disconnect Gas Inlet Power Entry Port Figure 5 TurboVap 96 Hardware Rear View Alarm Volume Control Adjusts the loudness of the beeper Service Panel Used by service personnel to access the exhaust blower and the sensor interconnect module Quick disconnect Gas Inlet Connects the gas supply to the workstation The fitting has an internal shutoff that allows you to disconnect the workstation from the gas supply without shutting off the gas supply itself Exhaust port Allows you to connect a hose to channel the solvent vapors to a
8. and the light will turn off lower than the previous setting Heater over heating Contact Biotage see page 3 due to a defective relay or temperature sensor hi displays in Cycle Plate temperature Contact Biotage see page 3 Readout mode sensor detects a temperature greater than 90 C or the temperature sensor has failed Fan is inoperative for Contact Biotage see page 3 this position lo is displayed in Cycle The temperature Contact Biotage see page 3 Readout mode sensor has failed Low Flow light is on The gas flow regulator Turn on the gas flow regulator and the alarm continues for the correct position and restart the position using the to beep has not been turned Start Stop button on The flow rate is set Adjust the flow rate and check below 5 or has the input gas supply dropped below 5 SCFH because the gas is running out Problem with flow Contact Biotage see page 3 sensor Elapsed Time displays The position has been Restart the position 11 3 in Cycle Readout stopped or is not mode started P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Troubleshooting 39 rs ale Time displays Time is set to This is the normal display kaaa infinite mode 99 minutes LED out on front panel Faulty LED Contact Contact Biotage see page 3 see Contact Biotage see page 3 3 Value or Units display Faulty display chip C
9. elapsed time displays P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Hardware 18 Cycle Readout when lit all of the parameters one at a time for any position that is running are displayed If neither position is running the display shows the set values for both positions The plate temperature display shows the present temperature when evaporation is in process for that position otherwise it shows the set value Select Button Advances the display indicator to the next option If you press the Select button after turning on the workstation the TurboVap enters diagnostics mode displaying the current firmware revision Set Button This button sets currently selected setting Gas Time or Plate Temperature to the displayed value The Set button only sets the Gas Time and Plate Temperature The Set button has no effect on the other settings Up and Down Arrow Buttons Increase or decrease the value in the display Used to set the values for Gas Time and Plate Temperature Start e Stop Buttons Start or stop evaporation During evaporation the Position light is green Over Temp Light Turns on red when the present temperature of one of the position is 10 C greater than the set plate temperature When the temperature drops below this value the light turns off The light may indicate a heater runaway caused by a defective relay or temperature sensor Low Flow Light Indicates a low gas flow rate
10. information or to obtain a quotation for services contact the Depot US 1 800 446 4752 or 1 pointsupport biotage com Europe and Rest of the World 46 18 56 57 11 or 1 pointsupport eu biotage com Japan 81 422 28 1233 or JP 1 Pointsupport biotage com Features of the Biotage Product Repair Depot e Factory trained repair technicians e Two week turnaround from receipt at Biotage P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Preface 4 Shipping Customers are responsible for shipments both to and from Biotage specifying the carrier and choice of service Return Policy To ensure a safe environment for all our technicians it is mandatory for each returned product to include our chemical questionnaire stating contact chemicals chemicals used during application and cleaning steps taken prior to shipment Processing Once the product is returned it is evaluated for necessary repairs The customer is contacted with an estimate and may choose to go ahead with the repair or decline service If service is denied a minimum evaluation charge may apply Upon completion of the repair a purchase order or appropriate means of payment is required before return shipment Service and Customer Support Plans FCC Biotage offers a full range of services to ensure your success From our original factory warranty through a comprehensive line of customer support plans Biotage AB offers you Field Service Engineers and In house Spec
11. specifications 41 Exterior cleaning 34 F Flow rate determining 26 DO 1 17 setting for position 1 28 setting for position 2 30 troubleshooting 37 Front panel ec ec 26 2464404064544 stiis 17 display problems 38 re Eie E she 17 Fuses INCIUABA ses cos 21 replacing oo specifications 40 G Gas automatic shut off 11 carbon trap 20 connecting 22 inlet location 15 input specifications 41 recommended pressure 20 regulator knobs 13 required 19 supply fitting required 41 supply input requirements 20 Gas TIOW ses sus RSE OS 10 considerations when setting rate 26 determining rate 26 rate too IOW 37 Biotage AB setting flow rate for position 1 28 setting flow rate for position 2 30 Gas nozzle manifolds 10 13 Gas supply maintenance 34 Gas time EE ae eae oe hee ee eee 32 fan sa 445045424440 roce y NOM ick dao he EP ee 17 setting for position 1 27 Gas vortex shearing action 10 H Hardware 13 cover open 14 front view 13 left side view 16 rear VIeW 15 Heater ASSEMDIY wceeeicec ea ee
12. suitable outside vent location Power entry port Connects the power cord and houses the fuse s P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Hardware 16 TurboVap 96 Left Side Position 1 Gas Regulator Figure 6 TurboVap 96 Hardware Left Side View Power Switch Power Switch Turns the power on and off Microplate Adapter Figure 7 Microplate Adapter Microplate Adapter Used to raise microplates up to the proper height Not used with deepwell plates P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Hardware 17 Front Panel TurboVap 96 Workstation Position 1 Position 2 O Figure 8 TurboVap 96 Front Panel Display Indicator Lights Indicate which setting is displayed in the Value and Units displays Gas Time displays the length of time to evaporate from 1 to 99 minutes 00 evaporates until manually stopped Plate Temperature the temperature in degrees Celsius of the airflow under the plate Range is from 20 ambient to 80 C Flow Rate displays the total gas flow for all 96 nozzles in each plate position Range is from 5 to 99 standard cubic feet per hour SCFH Adjust the flow rate using the gas regulators on each side of the TurboVap 96 Elapsed Time the time in minutes that has elapsed since the start of evaporation means not started Range is from 0 to 99 minutes If the Gas Time is set to manual mode 00 after 99 minutes the
13. the Plate Temperature for position 1 1 Press the Select button until the Plate Temp light for Position 1 is lit The display blinks and shows the previous temperature set for position 1 Press the up 4 or down arrow buttons to increase or decrease the temperature The Plate Temperature can be set from 20 C ambient to 80 C The Units display shows C The plate temperature is maintained within this setting 5 C Press the Set button to save the new temperature and display the flow rate for position 1 If you press the Select button before pressing the Set button the plate temperature is reset to the original value and the Flow Rate for position 1 displays During evaporation the display shows the actual temperature of the position not the set temperature NOTES The cooling effect of both the gas flow and the evaporation of the solvent will keep the internal temperature below the set plate temperature The temperature of each well during the evaporation process will be below that of the plate temperature Once the liquid in the well evaporates and the gas flow stops the well temperature will rise Setting the Flow Rate for Position 1 The flow rate is the total flow for the 96 nozzles in Position 1 it is not a per nozzle flow rate Change the flow rate by adjusting the gas regulator knobs on the sides of the TurboVap 96 To set the Flow Rate for position 1 1 P N 128097 Rev 00 Press the Select button
14. until the Flow Rate light for Position 1 is lit The display blinks and shows if the gas is off the cover is open or the left gas regulator knob is closed The Units display shows Fh for Standard Cubic Feet per Hour SCFH Make sure the source input gas is on Close the cover and press the Position 1 Start Stop button to turn on the gas to position 1 Pull the left Gas Regulator Knob out TurboVap 96 User s Manual Biotage AB Operation 29 Setting the Flow Rate for Position 1 Continued 5 Turn the gas regulator knob to adjust the flow rate as desired The flow rate can be set from 5 to 99 SCFH The Set button and the up and down buttons have no effect on this setting NOTE Turn the gas regulator knob slowly The flow sensors are very sensitive and may have some delay before equalizing 6 Once adjustments are made push the Gas Regulator Knob back in Press the Position 1 Start Stop button to turn off the gas Press the Set button If you press the Select button the Elapsed Time light for position 1 lights and Position 1 elapsed time is displayed Setting the Gas Time Evaporation Time for Position 2 Setting the Gas Time for position 2 is similar to setting the gas time for position 1 1 Press the Select button until the Gas Time LED light for position 2 is lit Press the up 4 or down y arrow buttons to display the desired time Press the Set button to save the selected time or p
15. AS 43 P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Introduction 10 Introduction The TurboVap 96 Concentration Workstation is a microprocessor controlled instrument that provides simultaneous automated concentration of multiple samples unattended operation convenience and speed The TurboVap 96 can simultaneously process one or two 96 well microplates or deepwell plates Figure 1 TurboVap 96 Gas Vortex Shearing Action The TurboVap 96 uses a patented gas vortex shearing action to maintain high evaporation rates under mild thermal conditions When you close the cover 96 gas nozzles in the manifold align with each well of the microplate or deepwell plate A helical flow of air is created by the stream of gas directed into each well The helical flow sets up a vortexing action that provides for sample homogeneity and continuous rinsing of the well wall The TurboVap 96 enables you to independently control the gas flow rate for Position 1 and Position 2 Gas Manifold D E Microplate Well Gas Vortex Shearing Action Figure 2 Gas Manifold Nozzle in Microplate Well P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Introduction 11 Microplate Heating Heaters below the microplate locating plates direct hot air through holes in the microplate locators toward each well in the microplate The forced hot air and the circulating gas quickly evaporates the solvent in each well Vapor R
16. N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Operation 32 Elapsed Time Display The Value display blinks when the position has been stopped or has not been started If evaporation has started the Value display shows the number of minutes since the start of the evaporation The maximum elapsed time is determined by the Gas Time setting which can range from 1 to 99 minutes If the Gas Time is set to 00 the elapsed time displays after 99 minutes The Set button and the up and down buttons have no effect on the elapsed time display If you press the Select button the Cycle Readout light is lit and the display rotates as described below Cycle Readout When Cycle Readout is selected the Value and Units displays show the current conditions for each parameter in the following sequence e Position 1 Gas Time e Position 1 Plate Temp e Position 1 Flow Rate e Position 1 Elapsed Time e Position 2 Gas Time e Position 2 Plate Temp e Position 2 Flow Rate e Position 2 Elapsed Time After displaying the Position 2 Elapsed Time the display cycles back to Position 1 Gas Time If evaporation is in process the current plate temperature is displayed not the set Plate Temperature If the current temperature is 10 C from the set Plate Temperature the displayed temperature blinks If only one position is running the display only shows the settings for that position If both posi
17. P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Preface 6 Table 1 Important Symbols Continued Symbol Symbole Description Description Risk of puncture injury Risk of eye injury wear safety glasses Risque de blessure par piq re Risque de l sion oculaire porter des lunettes de s curit Risk of exposure to biohazards Risque d exposition biohazards Risk of fire Risque d incendie Risk of poison Risque d empoisonnement Risk of explosion Risque d explosion Hazardous fumes manations dangereuses Hot surface risk of burns Surface chaude risque de br lures Protective ground symbol Symbole de terre de protection Ground symbol Symbole de terre Fuse Fusible Alternating current Courant alternatif On supply Marche alimentation UAAP PEPPER Off supply Arret alimentation CE compliance mark Marque de conformite CE Signifies that the unit has passed safety tests for grounding power line transience and current leakage Signifie que l appareil a r ussi les tests de s curit pour la mise a la terre le courant transitoire de ligne d alimentation et la perte de courant P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Preface T Table 1 Important Symbols Continued Symbol Description Symbole Description Input Lo Entr e Output io Sortie Equipment Caution risk of danger labels are color Stop coded M
18. andatory action Les tiquettes Safe condition or information de l appareil Attention danger potentiel sont cod es Arr ter couleur Intervention obligatoire Condition s re ou informations de s curit P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Table of Contents 8 Table of Contents Preface cit signin AN AN AA Te 2 Mad Kolo KN AA at eset Paes eee a etre stant ci 10 Gas Vortex Shearing ACUOA aaa de Ne eee Nu ten 10 Microplate Heating reus AA aa AA ces ecrit 11 VAD OK REMOVE 11 Automated Evapora ON aaa a aa aaa cr Rs Lan dre 11 A nd ee Ane tee nets en eue ed ru ects 11 CONVENIENCE sn ai enr en teens Di Re en Et desc RA 11 MG CONdILONS ais AAA AA E 11 Mstan ON MGA baa AGANG nie asia nier AA ces patate ice 11 COVE BENSON aa ST KATANA cet or Ceci On nine 11 Requires NO EXNAUST AOO miis dias 12 Multiple Sample PrOCeSSING suspicacia 12 Microplates and Deepwell Plates a 12 PUAN WANS EST ce aa miu nas 13 TUDO Vel 09 TONNES naaa 13 TurboVa p 96 Gover OPEN ainia 14 TUKOY Ap 96 REAN nia 15 TUDO Wap 90 Len SIde cia cola 16 Microplate Adapter ada ANA EU NE tn AR 16 AA PAA A tn ins 17 aianei IEY e a osa 19 WADAGKING ANG TUDO ap IO nta cea a 19 Parts Upper rune one 19 Items Required But Not Included 2 X Xa 19 Site Preparat ON saer A en 20 Imstalling tne TUDOVapYO curar dad 22 Connect the Gas Supply sainan Ea E E E E 22 Install the Venting Hose Re 23 Connect
19. ea ex 14 IK AN 11 setting temperature 28 29 Helical flow 10 loser eee oe bese we ee 38 l Indicator lights 17 Installation 19 Installing gas lines 22 TurboVap 96 22 Introduction 10 L LPA PPT AS 38 Low Flow light 18 troubleshooting 38 M Maintenance 34 ACI irritante eri 34 FOUR es de ae cu 34 Microplate adapter 14 16 Microplates 14 CAMUS Lost 12 specification 40 Multiple samples 12 ISP Ser cue deen tees AS ewes 32 P N 128097 Rev 00 TurboVap 96 User s Manual Index 44 N Noise level 42 O Operation 27 display during operation 32 preparing 27 Over Temp light 18 troubleshooting 38 p PONS an keene detre 13 cover open 14 front View 13 left side view 16 rear VIeW 15 supplied 19 Pausing concentration 33 Plate capacity 40 Plate temperature considerations when setting 25 determining 25 WOM RE 17 setting for position 1 28 setting for position 2 29 with cover open 25 Plates supported
20. emoval The solvent vapors are removed by a blower at the front of the TurboVap 96 that blows air across the top of the microplates and through the exhaust port at the back of the workstation From the exhaust port the venting hose must be routed to a suitable outside ventilation system Automated Evaporation The TurboVap 96 uses an integrated microprocessor to automatically time the evaporation control the temperature monitor gas flow rates automatically turn off the gas and heaters and perform operational diagnostics Adjustable The TurboVap 96 enables you to concentrate for a set time concentrate until stopped manually and optimize the concentration rate by adjusting the gas pressure and temperature Convenience You can start a run of samples and leave the instrument unattended When the concentration has finished the gas flow turns off automatically the temperature drops to 37 C and the workstation sounds an alarm every 30 seconds until you remove the samples or press the Start Stop button Mild Conditions The heaters provide sufficient warming without harming the sample Instant On Heat When you turn the workstation on the heaters heat up to the set Plate Temperature or to 37 C if the temperature is set above 37 C Cover Sensor A sensor in the cover turns off the gas to protect from gas vapors if the cover is opened during concentration P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Intr
21. entit responsable de la conformit peut annuler l autorisation d op rer l appareil accord e l utilisateur Table of Symbols Table 1 contains symbols that identify particularly important information and alert you to the presence of hazards These symbols may appear in this manual and or on the product it describes Table 1 Important Symbols Symbol Description Symbole Description DANGER An imminently hazardous situation which if not avoided will result in death or serious injury DANGER Situation pr sentant un danger imminent qui s il n est pas limin peut entra ner des blessures graves voire la mort WARNING Caution risk of danger Refer to the User s documentation AVERTISSEMENT Attention danger potentiel Se reporter a la documentation de l utilisateur NOTE A cautionary statement an operating tip or maintenance suggestion may result in instrument damage if not followed REMARQUE Enonc indiquant une pr caution a prendre un conseil de fonctionnement ou une suggestion d entretien son non respect peut provoquer des dommages a l instrument Hazardous voltage risk of shock injury Tension dangereuse risque de blessure par electrocution Crush hazard Risk of body parts hair jewelry or clothing getting caught in a moving part Danger d ecrasement Faire attention que les parties corporelles les cheveux les bijoux ou les v tements ne soient pas pris dans une pi ce mobile
22. ernal Temperature Two stations each adjustable from 20 C ambient to 80 C 5 C External Temperature Minimum Maximum Ambient 40 C 104 F Plate temp Plate temp lt 37 C System gt 37 C System State of Workstation Cover runs at runs at sal aa Evaporation gas time is Either open or closed Set temperature 37 C complete Gas Input O Evaporation has started Set temperature Set temperature O Use clean dry regulated compressed nitrogen or other suitable gas A carbon trap is recommended WARNING AAA Never use hydrogen or other flammable gases The wrong gas may explode or catch on fire Gas Supply Fitting 3 8 in 9 5 mm outside diameter Use the gas tubing supplied Inlet Pressure e Minimum 30 psi 2 1 bars e Maximum 80 psi 5 52 bars Exhaust Output Exhaust port 2 in 5 1 cm outside diameter Vent hose is supplied Vent hose 2 in 5 1 cm inside diameter supplied WARNING NA The exhaust duct MUST be routed to a suitable ventilation system vented outside the laboratory P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Specifications 42 Noise Level The workstation uses an internal exhaust blower Refer to the Declaration of Conformity for noise level test result P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Index A Adapter for microplate 14 16 Alarm volume control 15 Automated operation features 11 B
23. g the Start e Stop button P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Maintenance 34 Maintenance This section of the manual includes e Daily Maintenance on page 34 e Routine Maintenance on page 34 e Replacing the Fuses on page 35 e Long Term Storage on page 36 Daily Maintenance e During Power Up diagnostics confirm that all displays are accurate e Check that the tank has enough nitrogen for daily operation WARNING MA Never use hydrogen or other flammable gases The wrong gas may explode or catch on fire e Avoid spilling any solvents on the control panel e Avoid spilling any solvents on the heaters Routine Maintenance Panel Avoid contact with solvents and remove dirt buildup on the control panel To clean use a mild cleaning solution on a damp cloth Do not spray cleaner directly onto the control panel Exterior Clean exterior surfaces with a damp cloth and mild cleaning solution when necessary Cover Keep cover clean and do not use the cover as a shelf Gas Supply Periodically check gas hose and fittings for leaks Exhaust Port Periodically check for leaks in hose Replace if necessary P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Maintenance 35 Replacing the Fuses Fuse Requirements 100 120 VAC Model 220 240 VAC Model 1 6A F250V P N 38904 2 3 15A F250V P N 38902 WARNING AAA e Electrical shock hazard Turn the p
24. ialists who are dedicated to supporting your hardware software and application development needs US 1 800 446 4752 or 1 pointsupport biotage com Europe and Rest of the World 46 18 56 57 11 or 1 pointsupport eu biotage com Japan 81 422 28 1233 or JP 1 Pointsupport biotage com Our programs can include such useful services as e preventative maintenance e diagnostic servicing performed on site by Biotage AB field service engineers e extended use of the Biotage AB Customer and Technical Support Center e automated remote troubleshooting e software updates fter hour weekend and holiday support e repair depot servicing e parts labor and travel expense coverage e other customized services upon request This device complies with part 15 of the FCC United States Federal Communications Commission Rules Operation is subject to the following two conditions e This device may not cause harmful interference and e This device must accept any interference received including interference that may cause undesired operation P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Preface 5 CE This device complies with all CE rules and requirements NOTE Changes or modifications to this equipment not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment REMARQUE Tout changement ou modification apport cet instrument non express ment approuv par l
25. mpounds When selecting the Plate Temperature consider the following e Faster evaporation occurs as the plate temperature is increased however highly volatile analytes can be lost if allowed to sit for extended periods of time in a hot environment e You can use a Plate Temperature above the boiling point of your solvent if the gas flow rate is high enough to create a cooling effect over the solvent e The cooling effect of both the gas flow and the resulting evaporation of a solvent will keep the internal temperature below that of the set plate temperature e The temperature of each well during the evaporation process will be below the set plate temperature Once the liquid in the well evaporates and the gas flow stops the well temperature will rise When the workstation is turned on the plate temperature warms up to wait for the next evaporation process If the cover is opened after the evaporation process begins the following chart shows how the temperature changes Plate Temp Plate Temp State of setting is lt 37 C setting is gt 37 C workstation Cover system runs at system runs at Turned on and Either open or Set temperature 37 C initialized closed Evaporation has Closed Set temperature Set temperature started P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Determining Optimal Concentration Conditions 26 Plate Temp Plate Temp State of setting is lt 37 C setting is gt 37 C
26. n requirements Space Minimum bench space needed for the Concentration workstation and any accessories e Height 12 inches 30 5 cm e Width 23 5 inches 59 7 cm e Depth 12 5 inches 31 8 cm e Open Cover 21 inches 53 3 cm Work Area Flat level stable surface Indoor use only within 12 5 ft 3 8 m of proper ventilation Gas Supply You must use clean dry regulated nitrogen or other suitable gas Use the supplied 3 8 in 9 5 mm ID gas tubing for connection of the gas supply A carbon trap is also recommended 3 Use of compressed air could contribute to the oxidation of some phenolic compounds and reduce sample recoveries WARNING AAA Never use hydrogen or other flammable gases The wrong gas may explode or catch on fire Compressed Gas Supply Inlet Pressure Minimum Recommended Maximum 30 psi 2 1 bars 60 70 psi 4 1 4 8 bars 80 psi 5 52 bars 1 bar 100 kilopascals Devices added to the inlet supply line such as moisture traps or filters must not drop the pressure under 30 psi 2 1 bars measured at the TurboVap 96 inlet fitting P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Installation 21 WARNING MA To avoid injury to yourself or damage to the instrument DO NOT exceed 80 psi 5 52 bars maximum supply pressure Exhaust Duct The exhaust duct requires a 2 inch 5 1 cm diameter venting hose supplied The exhaust duct MUST be routed to a suitable ve
27. nical Support see Contact Biotage 1 Point Support on page 3 Troubleshooting the Concentration To diagnose problems related to the concentration process locate the description of the condition in the table below Concentration rate is too Low plate temperature Increase the Plate Temperature slow Gas flow rate is set too Increase the Flow Rate for the low or wrong gas correct position regulator knob is on Concentration rate is too High plate temperature Decrease the Plate Temperature fast Gas flow rate is set too Decrease the Flow Rate high No concentration at only Plugged nozzle Contact Biotage see page 3 one microplate well Sample is splashing Gas flow rate is set too Decrease the Flow Rate high Low recovery of sample Sample left too long At the end of concentration after completion of remove the sample promptly concentration Sample splashing Decrease the gas Flow Rate P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Troubleshooting 38 Troubleshooting the Front Panel Display To diagnose unexpected behavior of the panel display locate the description of the condition in the table below Plate Temperature Present temperature is Normal operation blinks during Cycle 10 C from the set Readout mode temperature Over Temp light is on Temperature for one of Allow time for the heater to go the positions has been down to the new temperature reset 10 C or more
28. ntilation system vented outside the laboratory WARNING AA Exhaust gases may be hazardous Consult the Material Safety Data Sheets MSDS for all of the solvents used Power AN 100 120 VAC Model 220 240 VAC Model Input Voltage 100 120 VAC 10 220 240 VAC 10 Line 50 60 Hz 50 60 Hz Frequency Fuse 1 6A F250V 2 3 15A F250V P N 38904 P N 38902 Ground The 3 prong power cord plug supplies the ground P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Installation 22 Installing the TurboVap 96 Connect the Gas Supply WARNING Never use hydrogen or other flammable gases The wrong gas may explode or catch on fire To connect the gas supply 1 Turn off the gas supply 2 Determine if an in line carbon trap is necessary 3 Place one of the supplied clamps over each end of the 3 8 in 9 5 mm tubing supplied 4 Connect one end of the tubing to the gas supply and tighten the clamp to secure the tubing onto the barbed fittings 5 Connect the other end to the barbed fitting on the Coupling Insert and tighten the clamp 6 Snap the Coupling Insert O ring end into the Quick disconnect Gas Inlet on the back of the TurboVap 96 7 Turn on the gas supply 3 8 ID Gas Tubing Barbed End A Clamp Side view of back of Concentrator Coupling Insert Quick disconnect with internal shutoff Gas Inlet Figure 9 Connecting the Gas Supply P N 128097 Rev 00 TurboVap 96 U
29. oduction 12 Requires No Exhaust Hood The cover and exhaust system permit the unit to be placed on a bench rather than taking up valuable hood space Multiple Sample Processing Each sample position provides 96 gas manifold nozzles that extend into the wells of the microplates or deepwell plates to supply gas for concentration Using both position 1 and 2 allows up to 192 samples to be processed simultaneously Microplates and Deepwell Plates Either one or two 96 well microplates or deepwell plates can be used Deepwell plates are placed directly on the surface of the locator plate Microplates use the supplied adapter to position the top of the microplate at the same level as the top of a deepwell plate P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Hardware 13 Hardware TurboVap 96 Front Cover Front Panel Position 1 i _ Position 2 Gas MENO Fe Gas Regulator Regulator Figure 3 TurboVap 96 Hardware Front View Front Panel Controls the setup and operation of the Concentration Workstation See Front Panel on page 17 for details Cover Holds the gas manifold that directs the gas into the wells of each plate to create the gas vortex shearing action Gas Regulator Knobs Set the flow rate for all 96 gas manifold nozzles The left gas regulator knob controls the flow rate to Position 1 the right knob to Position 2 Pull out knob turn then push knob in to make adjustments P N 128097
30. ontact Biotage see page 3 does not work Workstation Not Operational To diagnose problems with the unit locate the description of the condition in the table below Unit is inoperative Power is off or the Check that the power switch is power cord is loose on and that the power cord is seated MI Faulty fuse s Replace the fuse s O Faulty conta board Contact Biotage see page 3 Flow Rate is not Bad sensor PCB Contact Biotage see page 3 operational or not correct P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Specifications 40 Specifications Capacity One or two 96 well microplates or deepwell plates Physical Specifications Height 12 inches 30 5 cm Width 23 5 inches 59 7 cm Depth 12 5 inches 31 8 cm Height with cover open 21 inches 53 3 cm Weight 50 Ibs 22 7 kg Environmental Operating Temperature 59 to 95 F 15 to 35 C Operating Humidity 0 to 85 relative humidity non condensing Storage Temperature 50 to 122 F 10 to 50 C Storage Humidity 0 to 85 relative humidity non condensing Altitude Up to 2000M Power Input Voltage 100 120 VAC 10 220 240 VAC 10 Line Frequency 50 60 Hz 100 120 VAC 1 6A F250V P N 38904 220 240 VAC 2 3 15A F250V P N 38902 1 to 99 minutes with a one second resolution Time Range P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Specifications 41 Temperature Control Int
31. ower switch off and disconnect the power supply before changing the fuses e For continued fire protection replace fuses only with ones of the same type and rating To replace the fuses 1 Turn OFF the AC power and unplug the power cord 2 Locate the Power Entry Port fuse holder on the side of the unit 220 240V units have two fuse holders in the power entry port Always replace both fuses at the same time 3 Use a flat blade screwdriver to turn the fuse holder s from horizontal to vertical as shown in Figure 12 Fuse Holder locked Fuse Holder unlocked Fuse Holder r7 Da Fuse Lea om Figure 12 Replacing the Fuses 4 Remove the fuse holder and fuse 5 Remove the fuse from the fuse holder and replace it with one of the same type and rating 6 Replace the fuse holder s in the power entry port 7 Plug in the power cord and turn on the power P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Maintenance 36 Long Term Storage P N 128097 Rev 00 If the TurboVap will be stored for an extended period of time 1 Turn the unit off and unplug the power cord 2 Remove plates 3 Turn off and disconnect the air gas supply 4 Close the cover 5 Cover the unit to prevent dirt or dust accumulation TurboVap 96 User s Manual Biotage AB Troubleshooting 37 Troubleshooting This section provides charts for troubleshooting your concentrator If the solution listed in the chart is to contact Biotage Tech
32. possibly because the gas is running out or the flow regulator was not adjusted before starting the evaporation If the flow rate drops below 5 SCFH when the evaporation is in process the Low Flow LED lights and the alarm beeps continuously P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Installation 19 Installation This section describes how to unpack the TurboVap 96 how to prepare the site and how to install the TurboVap 96 Unpacking the TurboVap 96 Unpack the shipping container and verify that all parts listed below are included If any part is damaged or missing contact Biotage see Contact Biotage 1 Point Support on page 3 Parts Supplied The TurboVap 96 includes the following parts e Concentration Workstation e Power Cord s 220V models include two power cords one for EU and one for UK e Venting Hose 12 5 ft 3 8 m e 3 8 in 9 5 mm ID Gas tubing e Bag of two 3 8 in 9 5 mm Barb Fittings 1 8 in NPT and 1 4 in NPT e Coupling Insert e Two hose clamps e User s Manual CD e Microplate Adapters 2 Items Required But Not Included e Inert gas supply nitrogen with two stage regulator P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Installation 20 Site Preparation Before installing the TurboVap 96 Concentration Workstation you must have an appropriate location with available gas and electrical sources and adequate ventilation as specified by these site preparatio
33. prepare to start a concentration 1 Check that the incoming gas supply is turned on and has sufficient reserve for the sample run With the cover closed turn on the power The Value and Units displays show 88 and all LEDs turn on for 1 5 seconds After initialization the Position 1 Gas Time LED turns on and the Gas Time set for Position 1 displays Set the desired Gas Time Plate Temperature and Flow Rate for Position 1 and or Position 2 as described below Setting the Gas Time Evaporation Time for Position 1 After initialization is complete the Gas Time light for position 1 is lit and the Value display blinks and shows the number of minutes the evaporation will run To set the Gas Time 1 P N 128097 Rev 00 Press the up 4 or down y arrow buttons to increase or decrease the time The Gas Time can be set from 1 to 99 minutes and has a one second resolution Setting the Gas Time to 00 indicates manual operation If you set the Gas Time to 00 you must manually stop the gas flow using the Start e Stop button When the desired Gas Time is displayed press the Set button The selected Gas Time is saved and the Plate Temperature for position 1 displays If you press the Select button before pressing the Set button the time is reset to the original value and the Plate Temperature for position 1 displays TurboVap 96 User s Manual Biotage AB Operation 28 Setting the Plate Temperature for Position 1 To set
34. ress the Select button to reset the time to the original value The Plate Temperature for position 2 is selected Setting the Plate Temperature for Position 2 Setting the Plate Temperature for position 2 is similar to setting the Plate Temperature for position 1 1 P N 128097 Rev 00 Press the Select button until the Plate Temp light for position 2 is lit The display shows the previous set temperature for position 2 Press the up 4 or down y arrow buttons to display the desired plate temperature TurboVap 96 User s Manual Biotage AB 3 Operation 30 Press the Set button to save the selected temperature or press the Select button to reset the temperature to the original value The Flow Rate for position 1 displays Setting the Flow Rate for Position 2 The flow rate is the total flow for the 96 nozzles in Position 2 it is not a per nozzle flow rate You change the flow rate by adjusting the gas regulator knobs on the sides of the TurboVap 96 not by changing the setting on the display 1 Press the Select button until the Flow Rate light for Position 2 is lit 2 Make sure the source input gas is on 3 With the cover closed press the Position 2 Start Stop button to turn on the gas to position 2 4 Pull the right Gas Regulator Knob out 5 Turn the gas regulator knob to adjust the flow rate as desired The flow rate can be set from 5 to 99 SCFH The Set button and the up and down buttons have no effect on this se
35. ser s Manual Biotage AB Installation 23 WARNING MA To avoid injury to yourself or damage to the instrument DO NOT exceed 80 psi 5 52 bars maximum supply pressure Install the Venting Hose If the TurboVap 96 is not placed under a hood install the venting hose using the following procedure 1 Slide one end of the venting hose over the exhaust port Exhaust port _ Figure 10 Installing the Venting Hose 2 Route the hose to a suitable outside vented location or hood Ensure that the ventilation system is operating whenever the TurboVap 96 is in use WARNING AA e Gas fumes exiting this instrument can contaminate the air Maintain proper ventilation e Use only a venting hose with appropriate length and internal diameter to avoid excessive pressure drop The following hoses can be used to vent the TurboVap 96 Maximum Hose Length Internal Diameter 12 5 ft 3 8m 2 inch 5cm I D 30 ft 9 1m 2 5 inch 6 4cm I D 60 ft 18 2m 3 inch 7 6cm I D P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Installation 24 Connect the Power Cord 1 Plug the power cord securely into the receptacle on the back of the unit Make sure that the plug is fully inserted 2 Connect the 3 pronged end to an appropriate power source WARNING AAA To avoid the risk of fire or electrical shock plug the power cord into a properly grounded outlet Turn On the Power To turn on the TurboVap 96 turn
36. tions are running or stopped the settings for both positions are displayed If you press the Select button when Cycle Readout is selected the display shows the previously set Gas Time for Position 2 and the Position 2 Gas Time LED will light The up down and Set buttons have no effect when Cycle Readout is selected P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Operation 33 Pausing the Concentration The gas flow and the evaporation time continue as long as the cover is closed When the cover is opened an alarm beeps for 1 second The evaporation timer and the gas flow pause and will not turn on while the cover is in the open position When the cover is closed the evaporation continues Restarting the Concentration If the evaporation has started pressing the Start Stop button stops the evaporation and the alarm beeps for 1 second Pressing the Start Stop button again resets the elapsed time to zero and restarts the evaporation process Completing the Concentration When the evaporation is complete the Position light blinks and the alarm beeps every 30 seconds until you press the Position Start Stop button or open the cover If you close the cover the position is turned off NOTES e The heaters will maintain the set Plate Temperature if it was set to below 37 C or it will go to 37 C if it is set above this value e Ifyou set the gas time to 00 you must manually stop the concentration usin
37. tting NOTE Turn the gas regulator knob slowly The flow sensors are very sensitive and may have some delay before equalizing 6 7 8 Once adjustments are made push the Gas Regulator Knob back in Press the Position 2 Start Stop button to turn off the gas Press the Set button If you press the Select button the Elapsed Time light for position 2 lights and Position 2 elapsed time is displayed P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Operation 31 Starting the Concentration After setting the desired Gas Time Plate Temperature and Flow Rate for Position 1 and or Position 2 you are ready to start the concentration 1 Place the microplate adapter into any position where you will be using a microplate 2 Place the deepwell plates or microplates in the desired positions Gas Manifolds 4 D 333321 Position 2 with microplate adapter Position 1 saa wo ome hiJ Figure 11 Inserting Microplates into the Workstation 3 Close the cover 4 To start the concentration in position 1 press the Position 1 Start Stop button To start the concentration in position 2 press the Position 2 Start Stop button 5 If desired you can adjust either Gas Regulator Knob during the concentration process Be sure to pull the knob out before making any adjustments to the setting NOTE 1 The Gas Time and Plate Temperature cannot be changed once the concentration has started P
38. ur TurboVap product has been designed with safety as a foremost consideration These products are equipped with a non arcing fan solid state electronics and provisions for vapor collection Do not change the configuration of the TurboVap 96 ventilation system Please consult with Biotage if you have any questions or concerns regarding this subject WARNING TurboVap products are NOT classified as Explosion Proof The power switch is an arcing source and could ignite explosive vapors if present Contact Biotage 1 Point Support Biotage 1 Point Support provides expert services including telephone troubleshooting of products repair instructions service dispatching and replacement part information Before you call Check the online Help and the manual for a probable cause and solution to your question Have the following information available for the customer support representative e Workstation serial number s e If applicable the error displayed on the LCD display To reach the Biotage 1 Point Support US 1 800 446 4752 or 1 pointsupport biotage com Europe and Rest of the World 46 18 56 57 11 or 1 pointsupport eu biotage com Japan 81 422 28 1233 or JP 1 Pointsupport biotage com Biotage Product Repair Depot The Biotage Product Repair Depot offers product repair services upgrades refurbishment and installation at reasonable costs and with quick turnaround for all customer owned equipment and accessories For further
39. with highly corrosive acids To reduce the risk of electric shock do not remove the cover No user serviceable parts are inside Refer to qualified service personnel if help is required e Use this product only in the manner described in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired AVERTISSEMENTS e Pour r duire le risque de choc lectrique ne pas retirer le couvercle Ce produit ne contient aucune pi ce pouvant tre reparee par utilisateur Au besoin confier l appareil a un r parateur qualifi e Ce produit ne doit tre utilis que comme d crit dans ce manuel Si cet appareil est utilis d une mani re autre que celle sp cifi e par le fabricant la protection fournie par l appareil peut tre entrav e P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Preface 3 Product Safety Warning The TurboVap 96 power switch is the same type of power switch used on most laboratory equipment such as gas chromatographs spectrophotometers liquid chromatographs and computers The TurboVap products are safely used in the laboratory when Good Laboratory Practices are followed as with any other lab equipment All fans are brushless motor fans and will not ignite vapors The TurboVap is not classified as Explosion Proof and its use is at the discretion and risk of the operator or laboratory supervisor manager Yo
40. workstation Cover system runs at system runs at Evaporation has Open Set temperature 37 C started Gas flow time is Either open or Set temperature complete closed Considerations When Setting the Gas Flow Rate When selecting the gas Flow Rate consider that e A higher flow rate can cause a faster evaporation rate e An excessively high flow rate can cause the loss of analyte due to splashing e For the best results use the highest gas pressure possible without causing splashing e As the sample volume decreases during concentration increasing the flow rate will reduce the overall concentration time e For proper operation the pressure to the workstation should be at least 30 psi 2 07 bars To avoid damage the supply inlet pressure must not exceed 80 psi 5 52 bars 60 70 psi 4 1 4 8 bars is recommended WARNING MA e To avoid injury to yourself or damage to the instrument DO NOT exceed 80 psi 5 52 bars maximum supply pressure P N 128097 Rev 00 TurboVap 96 User s Manual Biotage AB Operation 27 Operation This section describes how to operate the TurboVap 96 For each of the two positions you can choose Evaporation time from 1 to 99 minutes or until stopped manually Plate temperature from 20 ambient to 80 C Gas flow rate from 5 to 99 standard cubic feet hour SCFH You can start or stop each position at any time independent of the other position Preparing the Unit To
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