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Switchos II Microcolumn Switching Unit Manual

1. FIGURE 2 6 Pin Out INPUTS Connector To control the switching valves A and B and the SSV e Connect two outputs of the controlling instrument to the Switchos input pins VALVE A and VALVE B of the INPUTS connector FIGURE 2 7 e Connect the common terminals or the ground terminals of the controlling instrument to the corresponding GROUND pins of the INPUTS connector FIGURE 2 7 e Connect the SSV pin and the corresponding GROUND in the same way to another output of the controlling instrument FIGURE 2 7 Controlling VALVE A Controlling VALVE A l Instrument VALVE B pean VALVE B _ _ O ssy Ssv O GROUND GROUND Relay A Contact Closure Relay Output B TTL Open Collector Output FIGURE 2 7 Different Output Configurations of the Controlling Instrument User s Manual Switchos D850R3 2 7 Installation and Getting Started DANGER VALVE A B these two pins control the two switching valves An open contact closure or an inactive TTL or open collector output will drive the corresponding valve into position 1 2 while an active input will drive the corresponding valve into position 10 1 The current positions are indicated by the LEDs on the front panel FIGURE 1 2 SSV this pin is used to select the position of the SSV An active input signal shorter than 1 5 seconds will switch the valve to the next solvent channel from A to B B to C and finally from
2. The User Interface 3 2 Powering up the Switchos When the Switchos Advanced Microcolumn Switching Unit is powered up via the main power switch on the rear panel FIGURE 2 1 it will go through an initialization self test protocol During this period all LEDs on the front panel will be illuminated for a short period of time and a number of messages are displayed on loading pump indicating that various components are functioning properly After completion of this procedure the Main Screen appears on the display of the loading pump FIGURE 3 1 and the Solvent Selection Valve will be switched to channel A The LEDs indicating the current status of the Switchos e g LED 1 2 in the upper front panel indicates that valve A is in position 1 2 Note The initialization procedure takes a few seconds The instrument is ready to use when LED A of the SSV LEDs is illuminated Kernel Kernel Screen with V X XX version number v UltiMate Firmware Screen with v 0 500 000 Main Screen OFF 25 25 25 25 FIGURE 3 1 Loading Pump Start Up Screens The system can be switched into standby mode e g when not used over night by pressing the ON STANDBY push button FIGURE 3 2 for greater than 2 seconds This turns off the power to the loading pump illuminates the Standby LED and places the Switchos into standby mode When the system is in standby mode pressing the button for a short time will power on the system again and switch the Standby LED
3. a Pull out the fuse holder item 1 FIGURE 6 7 FIGURE 6 7 The Fuse Compartment b Replace the blown fuse by a fuse of identical rating 2 A Slo Blo fuse c Re install the fuse holder 6 10 D850R3 User s Manual Switchos II Maintenance and Troubleshooting 6 4 Troubleshooting Troubleshooting refers to the determination of the cause of a problem Since the Switchos Advanced Microcolumn Switching Unit is incorporated into an HPLC system the first step to determine if the problem is due to this instrument is to remove the unit from the system then perform an injection and compare the results from the two runs If the results are fine the problem is due to the switching unit In addition the typical system parameters e g flow rates and pressure of a pre concentration setup with trap column and separation column are presented in TABLE 6 3 TABLE 6 1 Troubleshooting the Loading Pump Problem Probable Cause Solution Flow delivery stops after 1 minute during purging error message Minimum pressure is displayed System pressure dropped below the minimum pressure Pmin set in SETUP menu PRESSURE LIMITS Set min value to O MPa to disable checking Flow delivery stops during purging error message Maximum pressure exceeded nn MPa is displayed System pressure exceeded the maximum pressure Pmax set in SETUP menu PRESSURE LIMITS or in monitor screen Open the
4. if not they are not clean they should replaced d Fill the solvent reservoirs with the mobile phases to be used for the application caution Caution Only use the shielded solvent reservoirs supplied with the Switchos CAUTION Caution Do not operate the He lines a higher pressure than 4 bar 60 PSI Close the He shut off valves when the system is in idle state or not used Note All four solvent bottles must be filled and purged even if the application requires less than four mobile phases to assure proper function of the system Fill solvent bottles that will not be used with methanol water 1 1 Note The solvents must be degassed via the He degassing technique described below If other techniques are used e g vacuum degassing the performance of the system will be seriously degraded and the performance specifications will not be obtained e Open the He Shut off valves by rotating the knob so that the line on the valve is vertical FIGURE 2 9 and open the He Regulating Valve for maximum sparging FIGURE 2 10A Allow sparging to continue for approximately 10 minutes at a rapid rate then lower the He flow rate to maintenance mode FIGURE 2 10B D850R3 User s Manual Switchos Installation and Getting Started Vent i He Shut Off Valve FIGURE 2 9 He Shut Off Valve Go of He AF outer CAUTION N n T k _4 Bottle must be ad g n Solvent Inlet A B FIGURE 2 10 A Rapid Sparging B
5. CHROMELEON software the flow rate of the loading pump as well as the position of the valves of the Switchos II can be programmed from the CHROMELEON software The Switchos II has to be installed as described in Chapter 2 The role of the valves of the Switchos II is dependent on the application for which the unit is configured e g if the FAMOS Microautosampler is included in the system The user should ensure that the electrical interface and the fluidic interface to other devices in the system meet the requirements of the analysis es Note All dialog boxes and information refer to CHROMELEON 6 5 SP3 In this discussion we will describe how to establish a typical column switching application with the following parameters e Sample load clean up time 5 min at a loading flow rate of 30uL min e Gradient analysis time 50 min User s Manual Switchos II D850R3 4 1 Software Control 4 2 Server Configuration Setup 4 2 1 Adding and Configuring the Switchos Il To communicate with the Loading Pump of the Switchos II Advanced Microcolumn Switching Unit a Start the CHROMELEON server and then start the CHROMELEON Server Configuration Select the name for the server if more than one is configured of which you want to modify the configuration Click the character in front of the server name to view its current configuration Server Configuration Chromeleon Server Configuration a 18 x Fi
6. lt D 232 10 O00000 Vv RS 232 1 0 Network o o o RS 232 2 5 I loading flow O to application Rear Panel L FIGURE 1 1 Schematic Diagram of the Switchos Il Advanced Microcolumn Switching Unit The Switchos II Advanced Microcolumn Switching Unit includes the following components e 10 Port Switching Valves low dispersion valves assure dead volume free connection of any Micro or Nano LC column e g Fusica NanoSeries Micro and Nano Precolumns etc Two fast motor driven actuators control the valve positions 6 port valves are incorporated into some systems 4 Way Solvent Selection Valve allows for the automatic selection of up to 4 different solvents without the need of pump shut down or tedious purging One fast motor driven actuator switches the valve into the selected position 1 2 D850R3 User s Manual Switchos II Introduction e Loading Pump designed for the use in the LC Packings UltiMate Capillary HPLC system the micropump guarantees highest performance and perfect compatibility It is fully controllable via the CHROMELEON software e Solvent Bottles and He Degassing System provides mobile phase to the system The Helium degassing system is provided to improve check valve reliability and diminish baseline noise e Integrated Valve Controller micro controller based electronics controls the valve actuators and monitors the position of the three valves valve A valve B solvent sel
7. 1 3 Loading Pump 7 2 General 7 2 1 Physical 7 2 2 Electrical 7 2 3 Communication 7 3 Options Switching Valves A 1 Overview A 2 Maintenance A 3 Disassembly Reassembly of the Valve A 3 1 Disassembly of the Valve A 3 2 Reassembly of the Valve Network Identification B 1 Identifying the Switchos II on the Network Cleanup of Proteins C 1 Overview Connecting Fused Silica Capillary D 1 Overview D 2 Fitting Assembly D 3 Using Long PEEK Hex Style Nuts D 4 Spare Parts Lists 7 1 7 1 7 1 7 1 7 1 7 2 7 2 7 2 7 2 7 2 A 1 A 1 A 2 A 2 A 2 A 3 B 1 B 1 C 1 D 1 D 1 D 2 D 3 D 4 User s Manual Switchos II D850R3 Table of Contents This page intentionally left blank iv D850R3 User s Manual Switchos II Warranty LC Packings Netherlands BV warrants that the products manufactured and sold by it to be free from defects in material and workmanship for normal use and service from the date of delivery to original purchaser for a period of one 1 year from the date of shipment This limited warranty does not cover and no warranty is provided for parts that by their nature are required to be replaced periodically as a function of use of the normal operation of the system These items include without limitation HPLC columns fuses tubing detector sources pump piston seals injector rotors check valves filters any software etc In addition damage due to corrosion misuse negligence
8. 4 bar 60 psi Close the He shut off valves when the system is in idle state or not used User s Manual Switchos D850R3 2 9 Installation and Getting Started 2 6 3 Sparging the Mobile Phase and Flushing the Solvent Lines Although the Switchos loading pump is controlled via the CHROMELEON software initial system preparation is done with the pump on an off line basis i e the pump is operated locally To prepare the system for a separation a Power up the Switchos and make certain that power is provided to the pump b Check that the pump head backflushing system is operating A solution of isopropanol water 1 1 is commonly used but other solvents can be used if any of the buffer components are not soluble in this mixture reduce the fraction of propanol If desired you can reduce evaporation of the backflushing solution by connecting the two backflush ports A 5 mL syringe is provided to backflush the head and fill the reservoir Note The pump head of the pump should be backflushed with propanol water 1 1 If crystalline materials are deposited in the pump head irreversible damage to seals and or the piston may result this will dramatically shorten the life of these components c Inspect all fittings If there is a salt deposit by a joint it is probable that a leak has occurred and the fitting should be cleaned and tightened When you tighten a fitting do not overtighten Check that the solvent filters are clean
9. Delivery and the Baseline Monitoring 4 8 4 3 5 Creating a Program File Using the Wizard 4 8 4 3 6 Creating a Program An Example 4 8 4 3 7 Changing a Program File 4 12 CHAPTER 5 Testing the Switchos II 5 7 5 1 Overview 5 1 5 2 Checking System Components 5 2 5 2 1 Solvent Bottle Caps and Degassing Unit 5 2 5 2 2 Solvent Selection Valve Basic Test 5 2 5 2 3 Solvent Selection Valve and Solvent Lines Fluid Path Test 5 3 5 2 4 Switching Valves Basic Test 5 4 CHAPTER 6 Maintenance and Troubleshooting 6 7 6 1 Overview 6 1 6 2 Maintenance 6 2 6 3 Replacing Major Components 6 3 6 3 1 Replacing the Sparging Filter Unit 6 3 6 3 2 Replacing the High Pressure In Line Filter 6 4 6 3 3 Removing the Pump Head 6 5 6 3 4 Disassembling the Pump Head Replacing Piston Seals 6 6 6 3 5 Replacing the Check Valve Cartridge 6 7 6 3 6 Removing the Side Panels 6 8 6 3 7 Removing the Loading Pump 6 9 6 3 8 Cleaning the 10 Port Valve 6 9 6 3 9 Replacing the Main Fuse 6 10 6 4 Troubleshooting 6 11 6 5 Spare Parts List 6 13 6 5 1 Major Items 6 13 6 5 2 Tubing Fittings and Bottles 6 13 6 5 3 10 port Valve 6 14 6 5 4 Filters Tools 6 14 6 5 5 Trap Columns and Column Accessories 6 14 6 5 6 Control Cables 6 15 ii D850R3 User s Manual Switchos II Table of Contents CHAPTER 7 APPENDIX A APPENDIX B APPENDIX C APPENDIX D INDEX Specifications 7 1 Analytical Specifications 7 1 1 10 Port Switching Valves 7 1 2 Solvent Selection Valve 7
10. If no additional COM port is available or if the Switchos Advanced Microcolumn Switching Unit is to be installed with a different or third vendor software package the switching valves on the Switchos can be controlled by a the event outputs of the Switchos Loading Pump b the Auxiliaries P5 outputs of the FAMOS Microautosampler To connect the INPUTS connector a Valves are controlled by the Loading Pump Connect the INPUTS connector to the EVENTS outputs of the loading pump using the connection cable P N 160172 presented in FIGURE 2 4 Switchos Events EVENT3 Micropump Switchos II GROUND EVENT3 Valve A EVENT6 Valve B EVENT7 SSV EVENT6 en a P EVENT8 Trigger GROUND Switchos INPUTS EVENT eS o e to MS or another device FIGURE 2 4 Cable to control Switchos by the UltiMate System b Valves are controlled by the FAMOS Microautosampler Connect the INPUTS connector of the Switchos the P5 AUXILIARIES connector of the FAMOS Microautosampler and the START IN input of the UltiMate UV Detector using the connection cable P N 160171 presented in FIGURE 2 5 FAMOS P5 Auxiliaries FAMOS Switchos Il AUX1 Valve A Ee AUX2 Valve B Switchos AUX3 SSV INPUTS AUX4 START IN UltiMate UV Detector START IN FIGURE 2 5 Cable to control Switchos by the FAMOS P5 Connector 2 6 D850R3 User s Manual Switchos Instal
11. Ix Insert Line Delete Line Clean Up Interpolate lt Back Cancel Help FIGURE 4 10 The Flow Gradient Options Box Iez Note The Flow Gradient box is not present if the isocratic option was selected in the Pump Options box in the previous step g Setup the pressure limits for the separation column and the UltiMate pump and define separation column parameters e g D length and stationary phase in the U tiMate Pump Options box FIGURE 4 11 n Wizard Ultimate Pump Options x r Column Select a combination of column parameters OR specify a user defined CRP value Internal Diameter 75um E Other 1000um Length 15cm Re Other 25cm Stationary Phase E 8_3um_1004 Other C18_5um_3004 CRP Ento r Pump Pressure Limits r Column Pressure Limits Lower Limit fo 0 0 0 400 0 bar Lower Limit fo 0 0 0 400 0 bar Upper Limit 400 0 0 0 400 0 bar Upper Limit f2oq 0 0 400 0 bar m Peak Parking T Enable Peak Parking via START IN input Reduce flow to fp cabled 0 00 100 00 lt Back Cancel Help FIGURE 4 11 The UltiMate Pump Options Box ae Note If you do not specify the Column parameters e g the option other is selected you need to enter a CRP Column Resistance Parameter value The CRP is required to calculate the master flow rate of the UltiMate Micropump h Specify the detector settings e g the data collect
12. Sparging Maintenance Mode Shield removed to provide clarity f Connect the 5 mL syringe to the purge outlet on the purge valve on the loading pump using 1 16 ID silicon tubing FIGURE 2 11 a OU Purge Valve Knob Outlet FIGURE 2 11 The Purge Valve g Open the purge valve on the loading pump by turning the purge valve knob FIGURE 2 11 approximately 1 turn counterclockwise h Press the PURGE key on the loading pump set the flow rate of the to 0 0 mL and select solvent channel A using the SSV button on the rear side Section 3 3 Withdraw solvent from bottle A using the syringe until no air is observed Repeat this process for all four channels User s Manual Switchos D850R3 2 11 Installation and Getting Started i Set the purge flow to 0 5 mL min Allow the system to purge for at least 3 min After line A has been purged repeat the process for all other lines j Close the purge valve on the loading pump by turning the purge valve knob clockwise k Close the He shut off valve s on top of the solvent bottles that will not be used the white line should be horizontal I Place the loading pump under computer control and deliver mobile phase through the entire HPLC system at the flow rate and from the solvent reservoir that are used for the initial conditions for the analysis that you intend to perform As the system is delivering mobile phase check for leaks monitor the baseline and check that t
13. Switchos INERT 10 pc long hex nut and ferrule with E groove 160693 1 16 Universal Fingertight Fitting with double ferrule for Inert valve 1 piece z 161000 1 16 Universal Fingertight Fitting with double ferrule for Inert valve 10 each 163915 PEEK ferrules with groove for inert valves 20 pcs Notes a PAEK Polyaryletherketone b PEEK Poly etheretherketone D850R3 User s Manual Switchos II Index 4 Way Solvent Selection Valve 1 2 10 Port Switching Valves 1 2 Cleaning 6 9 A 1 B Baseline Monitoring 4 8 c CHROMELEON Control 4 1 Server 4 2 Server Configuration 4 2 Starting 4 7 Using 4 7 Cleaning 10 Port Switching Valves 6 9 A 1 Cleanup of Proteins C 1 COMMUNICATION Connector 2 4 Configuration Instrument 4 7 Contact Closure 2 7 Contents of Manual 1 6 Control Panel 4 7 Connecting to a Timebase 4 7 D Degassing Technique 2 10 Unit Testing 5 2 Design of Switchos II 1 2 Disassemble Pump Head 6 6 E Electrical Connections With UltiMate Capillary HPLC and FAMOS Microautosampler 2 3 With Other HPLC Systems 2 7 Event outputs Switchos controlled by 4 4 Using the 2 6 F FAMOS Installing the Switchos II with 2 3 Fittings Assembly D 2 PEEK Fittings D 2 D 3 Flow Gradient 4 10 Flow Rate Setting 3 5 4 8 Fluidics Connections 2 9 2 12 Flushing the Solvent Lines 2 10 Formula 4 5 Front View 1 4 Fuse Replacing 6 10 l Initialization 3 2 Inert Version 1 6 Connectin
14. Valcon E2 Switching Time lt 165 ms channel to channel four LEDs 7 1 3 Loading Pump Pump Type reciprocating double piston pump Flow Rate Range 10 500 wL min Flow Rate lt 0 5 Accuracy Pressure Range _ 0 400 bar Pressure indication of u column pressure Monitoring Rapid Purge Valve Instrument CHROMELEON software or standalone Control User s Manual Switchos II D850R3 7 1 Specifications 7 2 General 7 2 1 Physical Dimensions 185 mm 7 3 in x 350 mm 13 8 in x 430 mm 16 9 in WxDxH 16 kg 35 Ibs 7 2 2 Electrical Requirements 2x Slow Blow 2A Input resistance 10 kQ max Voltage Input inactive 0 5 V min Voltage Input active 2 0 V INPUTS VALVE A absolute maximum ratings VALVE B min voltage SSV max voltage Note Maximum ratings are the extreme limits the input pin can be exposed to without causing permanent damage Do NOT connect voltages lower higher than those limits 7 2 3 Communication by RS 232 serial communication Switching Valve or A and B by relay open collector or TTL outputs e g from FAMOS or U tiMate Micropump by RS 232 serial communication Solvent or Selection valve by relay open collector or TTL outputs e g from FAMOS or U tiMate Micropump Loading Pump by RS 232 serial communication network 7 3 Options Inert Version all fluidic pathways made out of inert materials factory i e PA
15. accident alteration of the system or repair by an unauthorized individual is not covered by the warranty It is understood that the performance characteristics of the instrument require that the mobile phase be degassed with He as described in the User s Manual This warranty covers products sold under the LC Products trademark If a different warranty than the above is indicated in the sales literature the warranty indicated in the sales literature will prevail If the system includes equipment supplied by LC Packings but manufactured by a third party LC Packings makes no warranty of any kind express or implied including without limitation any warranty of merchantability or fitness for a particular purpose LC Packings will make available to you to the extent permitted the warranties of the manufacturer of the relevant equipment following your timely written request If any product covered by this warranty becomes defective during the warranty period it will be repaired or replaced by LC Packings at no charge to the customer the repair replace decision is solely at the option of LC Packings All warranty requests must be received by LC Packings during the warranty period LC Packings will pay for surface transportation to the applicable LC Packings Office North America Sunnyvale CA Europe and Asia Amsterdam the Netherlands if the instrument proves defective within thirty 30 days from the date of shipment this does not in
16. all packing materials be saved in the event that it is necessary to return any item to the factory Note When lifting the instrument from the shipping container make sure that the unit is kept upright Lift the unit by placing your hands under the instrument If there is external damage to the shipping box the damage should be reported to the shipping agent and LC Packings upon receipt of the goods If internal damage is observed or if any items are missing this should be reported to the shipping agent and to LC Packings as soon as it is observed Note If there is any apparent damage to the instrument the user should investigate the nature of the damage before plugging the unit into the mains to ensure that powering up of the instrument will not create a hazardous condition or damage internal components If the damage appears significant call LC Packings or its local representative before connecting the unit to the mains 2 2 D850R3 User s Manual Switchos Installation and Getting Started 2 3 Installing the Switchos with the UltiMate Capillary HPLC System and the FAMOS Microautosampler When the Switchos is used as a component in the LC Packings UltiMate Capillary HPLC system all instruments are controlled by the CHROMELEON software The loading pump will be included in the instrument network and the switching valves are controlled by a COM port of the PC 2 3 1 Electrical Connections All electrical connections ar
17. compositions to be used in the separation e After you have finished using the system flush it with a water methanol or water acetonitrile mobile phase before shutting it down e Solvent should be degassed by sparging with Helium e Make certain that the sample and the buffer are soluble in all compositions of the mobile phase that will be used in the separation This test should be run in a beaker or test tube so that particulate matter does not enter the system If any cloudiness is observed in the test the gradient should be adjusted and repeated cauTioN Caution It is strongly recommend that only bottled HPLC water and solvent be used If water from water purification systems is used polymeric contamination may seriously damage the column and the flow cell This is especially true if sample pre concentration or 2D separations are performed This polymeric contamination may also seriously damage the flow cell e g coating of the capillary walls D850R3 User s Manual Switchos CHAPTER 3 The User Interface 3 1 Overview This chapter describes the general mode of operation of the Switchos Advanced Microcolumn Switching Unit It includes the following information e Powering Up the Switchos Section 3 2 e A description of the controls on the front and rear panel Section 3 3 e LOCAL vs REMOTE Control Section 3 4 e Basic Operations for the Loading Pump Section 3 5 User s Manual Switchos D850R3 3 1
18. off ON Standby Push Button with LED FIGURE 3 2 The ON Standby Switch 3 2 D850R3 User s Manual Switchos The User Interface 3 3 Controls on the Front and Rear Panel When the Switchos Advanced Microcolumn Switching Unit is used on a local basis the user sets the desired operating conditions via the controls on the front panel and the push button and switch located on the rear panel The present status of the system is indicated by a series of LEDs on the front panel The loading pump is controlled via the keypad on the lower front panel of the Switchos see Section 3 5 This section describes the use of the various controls and LEDs on the Switchos Basic information about how to use the keypad of the loading pump is presented in Section 3 5 and detailed information is presented in the UltiMate Micropump User s Manual The upper front panel of the Switchos FIGURE 3 3 includes the following ON STANDBY Switch He Regulating Valve LEDs Solvent Switehos Selection Valve z by LC Packings LEDs Valve A Valve A Button with LED Valve A LEDs Valve B Valve B A K 2 mie Microflow Outlet 8 Button with LED Valve B FIGURE 3 3 Control Elements on the Upper Front Panel e Valve A and B Controls the 10 port switching valves can be controlled by two push buttons when the Switchos is set into local mode The local mode is indicated by the built in LEDs Pressing the appropriate button will toggle the p
19. remove the Micropump a Disconnect all cables on the rear panel of loading pump b Remove the capillary inlet and outlet connections from the loading pump c Remove the side panels of the Switchos Section 6 3 6 d Remove the two screws on the left side and the two screws on the right of the loading pump e Remove the loading pump from the front of the Switchos caution Caution When removing the loading pump from the Switchos carefully pull the ae devices out of the housing To avoid any damage to the component make certain that it is not allowed to drop as it is being removed from the housing Note When re installing the instrument take care that the mobile phase tubing is not bent or overly flexed 6 3 8 Cleaning the 10 Port Switching Valve Cleaning the 10 port valve can often be accomplished by flushing all the lines with an appropriate solvent s The selection of the solvent is dependent on the nature of the sample and the mobile phases that are used Solvents such as methanol acetonitrile methanol water 80 20 or acetonitrile water 80 20 are normally used to clean the valve See Appendix A for instructions about disassembling assembling cleaning and maintenance of the switching valve User s Manual Switchos II D850R3 6 9 Maintenance and Troubleshooting 6 3 9 Replacing the Main Fuse DANGER Danger Disconnect the instrument from the mains before inspecting changing the fuse To change the Fuse
20. s Manual Switchos Installation and Getting Started Dimension Standard Version Inert Version 50 cm x 130 wm I D P N 160180 P N 160180 100 cm x 130 um I D P N 160181 P N 160181 b Connect port 6 of the injection valve of the FAMOS Microautosampler to the port 1 of valve A of the Switchos II as described in item a c Connect the outlet flow of the UltiMate System e g on the left side panel or from the upper T Piece of the flow splitter to port 4 of valve A of the Switchos Use the appropriate tubing for your application Application I D Standard Version Inert Version Capillary LC 50 um P N 161479 P N 161261 Nano LC 20 ym P N 160178 P N 161259 1 use PEEK fingertight fittings only d Connect port 3 of valve A of the Switchos to the UltiMate column bulkhead or directly to the micro column located in the UltiMate column compartment Use the appropriate tubing for your application Application I D Standard Version Inert Version Capillary LC 50 um P N 161480 P N 161262 Nano LC 20 um P N 160179 P N 161260 1 use PEEK fingertight fittings only e Connect ports 6 and 10 of valve A of Switchos to waste e g using 200 500 um I D PTFE tubing f Connect the trap column between ports 2 and 5 of valve A of the Switchos Il using the appropriate tubing supplied with the trap column A typically loading solvent is 100 water with 0 0
21. selective extraction Typical examples of the use of the unit which are described in Section 2 6 4 and Appendix C include immuno affinity extractions isolation of phosphorylated peptides from complex protein digests automated removal of detergents extraction of drugs from biological fluids high throughput analysis etc Switchos II is configured with an LC Packings UltiMate Micropump and is normally used with the LC Packings UltiMate Capillary HPLC System The system is fully compatible with other instrumentation a minimum of three contact closures TTL or Open Collector terminals are required User s Manual Switchos II D850R3 1 1 Introduction 1 2 General Design of the Switchos II Advanced Microcolumn Switching Unit A schematic diagram of general design of the Switchos II Advanced Microcolumn Switching Unit is presented in FIGURE 1 1 Solvent lines A D to the SSV L f 2 a ES f Solvent Bottles eee T MISS A D with Front Panel MAN a Bottle Caps a l AS D I He to C IHe Bottle Caps B Regulating A Valve i S ial Solvent Tray He Inlet i 4 way Solvent He Line I Check Valve i 10 port valve A Selection Valve A DO O OB RF ian O COMMUNICATION I C o i 10 port valve B ro Integrated Valve Remote ig h l Controller Control a J I INPUTS 1 Loading Pump O00000 l
22. the Valve To disassemble the valve a Use a 9 64 hex driver to remove the socket head screws which secure the cap to the valve FIGURE A 1 Driver Stator FIGURE A 1 Exploded View of the Valco Model C2 Valve 6 port Version b To insure that the sealing surface of the cap is not damaged rest it on its outer face If the tubing is still attached leave it suspended by the tubing c Gently pry the rotor away from the driver with your fingers or a small screwdriver d Examine the rotor sealing surface for scratches e f scratches are visible to the naked eye the rotor must be replaced e f no scratches are visible clean all parts thoroughly with an appropriate solvent Take care that no surfaces get scratched while you are cleaning the components Note The most common problem in the use of the valve with HPLC is the formation of buffer crystals which are usually water soluble After cleaning it is not necessary to dry the rotor D850R3 User s Manual Switchos II The Switching Valves A 3 2 Reassembly of the Valve To reassemble the valve a Replace the rotor in the driver making sure that the rotor sealing surface with its engraved flow passages is facing out The pattern is asymmetrical to prevent improper placement b Replace the cap Insert the two socket head screws and tighten them gently until both are snug caution Caution Do not overtighten the screws they simply hold the assembly togethe
23. 5 TFA Fill solvent bottle A with this solvent Fill bottle B with either the same solvent or a mixture of methanol water 1 1 bottles C and D may be left empty After pre concentrating the sample on the trap column the valve A of Switchos will be switched into position 10 1 to elute the sample FIGURE 2 13 FAMOS Analytical microcolumn port 6 Micro flow to UltiMate Switchos Valve A Waste ValveA 9 presented 4 in position 8 10 1 Trap column FAMOS port 1 6 O0000_A Micro flow 00000 v from UltiMate S o O Ko Waste FIGURE 2 13 Eluting Sample adsorbed on the Trap Column in Backflush Mode User s Manual Switchos D850R3 2 13 Installation and Getting Started 2 7 Routine Operation of the System 2 7 1 Sample and Mobile Phase Considerations The Switchos Advanced Microcolumn Switching Unit is used in an HPLC system and the standard operating precautions for HPLC should be employed e Ensure that samples and mobile phases do not contain particulate matter All samples and mobile phases should be filtered through a 0 22 um membrane filter If organic solvents are used make sure that extractable materials are not present in the filter e The sample should be soluble in the mobile phase If a gradient is used make certain that the sample is soluble in the mobile phase at all mobile phase
24. 50082 1 Generic immunity standard EN 61000 3 2 Harmonic current emissions ee Use shielded cables and connectors for all remote connections Amsterdam January 11 2001 D934R1 Robert van Ling QA manager User s Manual Switchos II D850R3 xi CE Declaration This page intentionally left blank xii D850R3 User s Manual Switchos II ere Introduction 1 1 Features of the Switchos II Advanced Microcolumn Switching Unit The Switchos Il Advanced Microcolumn Switching Unit is an advanced switching system for use with micro HPLC systems The system incorporates the following features e Two Valco 10 port low dispersion switching valves which allow for the connection of capillary micro and nano HPLC columns without any dead volume e A high precision loading pump fully controllable by the CHROMELEON software e Four channel solvent selection valve e Automatic control of all valves by the CHROMELEON software and manual control via push buttons e Manual valve switching is possible at any time even when a program is running e LED s which indicate the present status of the switching valves microfluidic pathways the position of the solvent selection valve and the control mode A broad range of applications can be performed using micro column switching with the Switchos II including sample preconcentration sample cleanup multi dimensional separations 2 D separations desalting and
25. 50R3 6 1 Maintenance and Troubleshooting 6 2 Maintenance Maintenance refers to a variety of activities that should be performed on a routine basis to optimize performance of the system Many routine maintenance activities can be readily performed by the user Detailed information about the maintenance of the Switchos Loading Pump is presented in the UltiMate Micropump User s Manual In some cases e g replacement of critical components we recommend that a factory trained service engineer should be called to perform the operation This will ensure optimal long term performance and maximum uptime LC Packings provides a broad range of service support activities to ensure that the Switchos Advanced Microcolumn Switching Unit is functioning in a suitable manner These activities can be customized to meet the specific needs of the customer For further information please contact your local LC Packings office or representative TABLE 6 1 Recommended Maintenance Schedule Frequency Operation Every Day Before operating check for any air bubbles in the fluidic lines and degas the solvent Check that the fluidics connections do not leak Check that salts are not deposited by the fluidics joints When using buffer solutions flush the system thoroughly after use with a solvent that does not contain buffers salts Every 3 months Inspect the condition of all tubing cracks nicks cuts clogging Every year Replace Rotor
26. Change Cancel Apply Help FIGURE 4 5 The Virtual Channel Driver Box b Check the first channel and click on Change to configure it To readout the column pressure configure the Signal Configuration box as indicated in FIGURE 4 6 al Configuration x Signal Name ColumnPressure Unit fbar Eactor fi Type Analog Digital Fixed Formula Pump Columnpressure Description Assign a unique symbolic name to the signal Cancel FIGURE 4 6 Virtual Channel Setup for Column Pressure Readout To monitor other parameters e g the UltiMate Micropump pressure select a new channel FIGURE 4 5 and configure the signal as presented in TABLE 4 2 User s Manual Switchos II D850R3 4 5 Software Control TABLE 4 2 Signal Name and Formula Definitions Device Signal Name Unit Factor Formula ULT PumpPressure bar 1 0 pump masterpressure ULT ColumnPressure MPa 0 1 pump columnpressure SW II TrapColumnPressure psi 14 5 loading_pump trapcolumnpressure ULT OvenTemperature oC 1 00 Oven Temperature FMS Tray Temperature Sampler Temperature Note a ULT UltiMate FMS FAMOS SW II Switchos II A typical setup is presented FIGURE 4 1 General Signals Error Levels Factor Type _ Formula PumpPressure bar 1 00 Analog pump masterpr ColumnPressure bar 1 00 Analog pump colurnnpr TrapColumnPressure bar 1 00 Anal
27. D back to A Additionally the SSV can be reset to solvent channel A by applying an active signal for longer than 1 5 seconds FIGURE 2 8 shows the timing diagram The selected solvent channel is indicated by one of the four LEDs on the upper front panel FIGURE 1 2 Step to next Reset to Channel Channel A O gt 999 py rrr rrr nn ee pn nn nee active ono inactive t ti 7s t2 t2 2s t2 3s T FIGURE 2 8 Timing Diagram of the SSV Input 2 5 2 Power Connector Connect the Switchos to the power line as described in Section 2 3 6 Danger The Switchos must be connected to a power source that is connected to a true ground D850R3 User s Manual Switchos Installation and Getting Started CAUTION CAUTION CAUTION 2 6 Fluidic Connections Switchos to the HPLC System 2 6 1 Preliminary Operations To connect the Switchos Advanced Microcolumn Switching Unit to an HPLC system and to prepare the instrument for operation e Place the Switchos Advanced Microcolumn Switching Unit in its operating location preferably on the left side of the HPLC system Make sure the ventilation holes are not obstructed Allow the instrument to acclimatize for 1 hour e Connect the Helium line Section 2 6 2 e Power up the system The display of the loading pump and the various LED s will indicate that the self test and initialization have been executed A detailed discussion about the control and the various di
28. EK PEEK PTFE ceramic titanium installed Flow Passage 0 25 mm Diameter 7 2 D850R3 User s Manual Switchos II Switching Valves A 1 Overview The LC Packings Switchos II Advanced Microcolumn Switching Unit is equipped with two Cheminert Model C2 10 Port Column Switching Valves manufactured by VICI Valco Instruments Co Inc These valves are designed to offer optimal results with the switching system as they are made from materials that are inert to materials used in liquid chromatography and are designed to provide exceedingly low carryover when switched eS Note A detailed discussion on the Installation Use and Maintenance of the valve is presented in Technical Note 801 from VICI Valco Instruments Co Inc and can be obtained at the Valco website www Valco com User s Manual Switchos II D850R3 A 1 The Switching Valves A 2 Maintenance In most instances the only maintenance that is required is cleaning of the valve Cleaning can often be accomplished by flushing all the lines with an appropriate solvent s The selection of the solvent is dependent on the nature of the sample and the mobile phases that are used Typically solvents such as methanol acetonitrile methanol water 80 20 or acetonitrile water 80 20 should be used A 3 Disassembly Reassembly of the Valve Note Do not disassemble the valve unless system malfunction is definitely isolated to the valve A 3 1 Disassembly of
29. EK Adapter 162300 15 Check Valve Bushing 161071 16 Set Screw 17 Backflushing Connection 18 Connecting Tubing Pump Head 162010 Stainless steel 161023 3 Titanium Notes 1 Set of 4 piston seals includes two high pressure seals and two backflushing seals 2 Used with the standard stainless steel version of the Switchos only 3 Used with the inert version of the Switchos only FIGURE 6 5 The Inert Pump Head Assembly b Loosen the two screws that attach the retaining plate to the pump head items 1 2 FIGURE 6 5 As you remove the screws alternate from one to the other as long as the springs are loaded to avoid damaging the retaining plate ae Note Since the springs item 5 FIGURE 6 5 exert a significant amount of force care should be taken when the screws are loosened 6 6 D850R3 User s Manual Switchos II Maintenance and Troubleshooting c Remove the spring guides the springs and the support washers items 4 5 7 FIGURE 6 5 d You can remove replace the piston seals backflushing seals now Use an appropriate tool e g a small screwdriver to remove the seals item 8 FIGURE 6 5 e Loosen the spacing bolts item 6 FIGURE 6 5 Remove the two spacing bolts and then remove the pressure plate item 9 FIGURE 6 5 f You can remove replace the piston seals high pressure seals now Use an appropriate tool e g a small screwdriver to remove the seals item 11 FIGURE 6 5 Ie Note The s
30. Manual Switchos II D850R3 4 3 Software Control 4 2 3 Switchos Il controlled by Event Outputs If serial control is not possible e g no free COM port available the Switchos loading pump can be used to control the valve positions The switching valves are then controlled by the event outputs of the Switchos II loading pump TABLE 4 1 and EVENTS8 can be used to trigger another device e g a mass spectrometer TABLE 4 1 Control Outputs Switchos II Input Loading Pump Default CHROMELEON Signal Name Valve A EVENT3 Valve A EVENT6 Valve B EVENT7 SSV EVENT8 Switchos Relay2 IES Note The EVENT number as well as the type are indicated on the rear panel of the Micropump e g EVENT 1 TTL EVENT 3 OC etc Make certain that the proper EVENT output is linked to the corresponding signal name of CHROMELEON e g Valve A should control EVENT3 of the Micropump SSV should control EVENT7 etc To control the unit by the event outputs of the Loading Pump a Select the Relays tab of the LC Packings U tiMlate Switchos box FIGURE 4 4 ings UltiMate S witchos General Components Flow Options UltiMate Solvents Signals Relays Inputs EmorLevels Name Function a C Switchos_TTL_2 Switchos Output 2 TTL M Valve_A Switchos Output 3 Open Collector L Switchos_TTL_3 Switchos Output 4 TTL C Switchos_TTL_4 Switchos Output 5 TTL M Valve_B Switchos Output 6 Open Collector M ssy Switchos
31. Output 7 Relay C Switchos_Relay_2 Switchos Output 8 Relay 4 gt Deactivate check boxes to remove unused relays from Online Hit F2 to edit selection FIGURE 4 4 The UltiMate Configuration Box Relays Tab b Check the outputs SSV Valve_A and Valve_B check Switchos_Relay_2 if you want to trigger any instrument by the EVENT 8 output IES Note The default signal names corresponds to the names used in the predefined panels and should not be changed If you modify the signal names they may not be recognized and some functions may not work properly 4 4 D850R3 User s Manual Switchos II Software Control 4 2 4 Adding and Configuring the Virtual Channel Driver To monitor and record various parameters of the UltiMate e g the column pressure or the oven temperature a Virtual Channel Driver is needed To add a Virtual Channel Driver a Click on Add Device in the Server Configuration box and select Virtual Channel Driver from the General tab to present the Virtua Channel Driver box FIGURE 4 5 General Signals Error Levels Factor Type Formula a Mf VirtualChannel_01 1 00000 Analog VirtualChannel_02 1 00000 Analog VirtualChannel_03 1 00000 Analog VirtualChannel_04 1 00000 Analog VirtualChannel_05 1 00000 Analog VirtualChannel_O6 1 00000 Analog VirtualChannel 17 10nn Analan Deactivate check boxes to remove unused channels from Online
32. Switchos I Advanced Microcolumn Switching Unit User s Manual P N 162013 Now sold under the Thermo Scientific brand Thermo SCIENTIFIC LC PACKINGS www lcpackings com D850R3 Notice The Switchos Il Advanced Microcolumn Switching Unit is covered by a limited warranty A copy of this warranty is included with this manual The customer is required to perform routine maintenance as described in the User s Manual on a periodic basis to keep the warranty in effect All information in this manual is subject to change without notice and does not represent a commitment on the part of LC Packings BV The material included in this manual is provided to assist users in the operation maintenance and repair of the Switchos Il Advanced Microcolumn Switching Unit It is assumed that the individual using this manual has sufficient training in the use of analytical instrumentation and is aware of the potential hazards including but not limited to electrical hazards chemical solvent hazards and the exposure to pressurized solvents October 2004 LC Packings BV A Dionex Company All rights reserved No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of LC Packings BV The following are registered trademarks of LC Packings A Dionex Company UltiMate UltiChrom FAMOS Switchos Probot wCarrier Thermos Acurate UZ View UV Booster FluoBoost Pepmap w Dump
33. Timebase a Click on the Connect to Timebase command on the Control menu to select the Timebase you want to connect the panel to User s Manual Switchos II D850R3 4 7 Software Control b Use on the Save as command on the File menu to save this new assignment 4 3 4 Starting the Flow Delivery and Baseline Monitoring To gain control of the different Ultimate modules each module of the configuration need to be connected to the control panel Check the Connect boxes to connect the individual modules item 1 FIGURE 4 8 Fie Edt vew Workspace Qualification Control Batch Window Help le x OSH 8S Telo iele gt at TEES Ome mm es amp kht ikh S x RA GORAM_local Run time et 311 19 Oven Tengerre Sequence manual 1 488 min a2 11 69 0 00UV ME 3 Mamelena 74 Curreen Sample Manual a emt 3 11 49 0 000 UV MS t Acqon Sonais uisition timel a 312020216 Louden Pure Flaw 0 030 Type Ueiknown Sample No 1 Ri 3 12 05 0 308 Losing Purp Flow 0 000 Ultimate pump Pocoma ERI toe on ef k Flow 0000 mimin E Flow 0000 mimin E mimin H _ Precolimn pressure 0 bar _ Precolimn pressure 0 bar 0 0 bar a a 2 6 min 897 nA Kaan aani oguisition FIGURE 4 8 The Control Panel for UltiMate FAMOS and Switchos Flow delivery and baseline monitoring can now be started Enter the column and the trap column flow rates in the Flow fields in the corresponding
34. UIRE PC has connected Server Configuration to this server F 11 54 08 User Administrator has disconnected Server Configuration from this server Fp 12 17 48 User Administrator from ESQUIRE PC has connected Server Configuration to this server 12 17 48 User Administrator has disconnected Server Configuration from this server F 12 18 28 User Administrator from ESQUIRE PC has connected Server Configuration to this server fF 13 18 28 User Administrator has disconnected Server Confiauration trom this server 9 15 57 56 User Adr SS fF 15 57 56 User Adr g peto tmebase xl f 15 04 56 User Adr 9 15 04 56 UserAdt Timebase Name DEMO F 15 17 39 User Adr F 16 17 39 User Adr F 16 17 39 User Adr FP 16 22 11 User Adr FP 16 22 44 User Adr FP 15 22 44 User Adr F 15 22 44 User Adr 2Ultimates g LC Packings Famos 2 LC Packings Switchos II 55V f LC Packings UltiMate Switchos virtual Channel Driver For Help press F1 Astar AA e 1 D X bo SG GEM Ae chromeleon fesquine B Server Configuration Q Bm Mme 4 22PM FIGURE 4 1 The Server Configuration Box b Double click on the LC Packings UltiMate Switchos device item 2 FIGURE 4 1 The LC Packings UltiMate Switchos box appears FIGURE 4 2 s UltiMate Switchos Signals Relays Inputs Error Levels General Components Flow Options UliMate Solvents COM Port z M UltiMate Pump Pump M UltiMate Detector IV Sw
35. Version 50 um I D Connecting tubing Switchos to UltiMate injection valve 70 cm length 50 um I D Connecting tubing Switchos to UltiMate column compartment length 50 cm includes union 30 wm I D PEEK shielded fused silica connection tubing x Precolumn Valve 161479 161480 160182 Nano LC Standard Version 20 um I D Connecting tubing Switchos to UltiMate injection valve length 65 cm 20 um I D Connecting tubing Switchos to UltiMate column compartment length 50 cm includes union 30 um I D PEEK shielded fused silica connection tubing yx Precolumn Valve Capillary LC Inert Version 50 um I D Connecting tubing Switchos to UltiMate injection valve 70 cm length 50 um I D Connecting tubing Switchos to UltiMate column 161262 compartment length 50 cm includes union 160182 30 um I D PEEK shielded fused silica connection tubing x 160178 160179 160182 161261 6 14 D850R3 User s Manual Switchos II Maintenance and Troubleshooting Precolumn Valve Nano LC Inert Version 161259 20 um I D Connecting tubing Switchos to UltiMate injection valve length 20 cm inert version 161260 20 um I D Connecting tubing Switchos to UltiMate column compartment length 50 cm includes union inert version 160182 30 um I D PEEK shielded fused silica connection tubing yx Precolumn Valve 6 5 3 10 port valve P
36. arging filter frits or bent solvent lines d Switch the flow delivery of the loading pump off disconnect the solvent inlet line from the pump head and connect a 250 uL syringe i e the plunger should be removed using an adapter P N 160259 as shown in FIGURE 5 2 FIGURE 5 2 Placing the Syringe on the Solvent Inlet Line e Select solvent channel A by the SSV button on the rear of the Switchos The LED corresponding to Valve A should be illuminated f Measure the flow rate from the inlet tubing for one minute The flow rate should be greater than 50 uL min for a solvent line that is functioning properly g Repeat steps e and f for each solvent line Note If the flow is less than the specified value repeat the test with the solvent filters removed If the flow is within specifications after the filters have been removed they are clogged dirty or defective and must be replaced Note As an alternative to using a syringe this test can be performed by allowing the solvent to drip from the solvent inlet line and counting the number of drops per minute At least 5 drops per minute should be observed if the proportioning valve opens properly If the flow rate is less than 5 drops min repeat the test with the solvent filters removed If the flow is within specifications after the filters have been removed the filters are clogged dirty or defective and must be replaced User s Manual Switchos D850R3 5 3 Testing the Swi
37. art Number Description Standard Version Stainless Steel 161472 Replacement rotor for VALCO C2 10 Port 162016 Stator VALCO C2 10 Port Inert Version PAEK 161005 Replacement rotor for VALCO C2 10 Port valve inert version 161006 PAEK Stator VALCO C2 10 Port valve inert version Note PAEK Polyaryletherketone PEEK Polyetheretherketone 6 5 4 Filters Tool Part Number Description 160044 He Sparging Filter Frit 160073 Socket driver 11mm for In line filter replacement 160259 Syringe adapter for Valco switching valves Standard Version 160177 0 5 um replacement in line filter frit 161001 Frit Holder Inert Version 161107 0 5 um replacement in line filter frit inert version 162014 Frit Holder Titanium inert version 6 5 5 Trap Columns and Column Accessories Part Number Description 160461 Nano trap column holder 1mm 160431 160432 Micro trap column holder 5mm Micro trap column holder 15mm Micro Trap Columns 160454 300 wm I D x 5 mm packed with 5 wm C18 100A PepMap set of 5 cartridges 160446 500 um I D x 5 mm packed with 5 um C18 100A PepMap set of 5 cartridges 160450 500 um I D x 15 mm packed with 5 wm C18 100A PepMap set of 5 cartridges Nano Trap Columns 160458 300 um I D x 1 mm packed with 5 um C18 100A PepMap set of 5 cartrid
38. ble and shall run to the benefit of the original purchaser only Any change alteration or amendment to this warranty is not valid unless it has been approved in writing by an officer of LC Packings North America Europe and Asia LC Packings Dionex LC Packings Netherlands BV A Dionex Company 500 Mercury Drive Abberdaan 114 Sunnyvale CA 94088 3603 1046 AA Amsterdam USA The Netherlands Technical Call Center Phone 31 20 683 9768 USA CA 800 346 6390 Fax 31 20 685 3452 vi D850R3 User s Manual Switchos II Instructions for Returning Instruments Before you return any item for repair please contact the nearest LC Packings office or its local distributor for instructions and obtain a return authorization number and the Health and Safety Form if applicable Pack the equipment carefully preferably in its original shipping container and ship it to the LC Packings Service Department using the appropriate address North America Europe and Asia LC Packings Dionex LC Packings Netherlands BV A Dionex Company 500 Mercury Drive Abberdaan 114 Sunnyvale CA 94088 3603 1046 AA Amsterdam USA The Netherlands Technical Call Center Phone 31 20 683 9768 USA CA 800 346 6390 Fax 31 20 685 3452 IMPORTANT 1 Make certain that the return authorization number together with the HEALTH AND SAFETY form if applicable is attached outside of the package so that we can properly track and account for your system 2 P
39. boxes item 2 FIGURE 4 8 and click on the blue circle in the toolbar to start baseline monitoring item 3 FIGURE 4 8 4 3 5 Creating a Program File Using the Wizard CHROMELEON programs are text files and modifying a program is normally done by editing in the text window When creating a new program file a Program Wizard starts automatically and guides you through the programming to simplify the procedure Enter the required information in most cases the pre defined values can be used The wizard provides a ready to use program based on your entries without the need to type a special programming syntax Press the F1 key for additional help and more detailed information Section 4 3 6 provides a programming example 4 3 6 Creating a Program An Example The following section provides information how to program the flow rate of the loading pump and how to use the switching valves D850R3 User s Manual Switchos II Software Control For a column switching experiment a pre concentration step the Switchos II Advanced Microcolumn Switching Unit the UltiMate system Nano configuration and the FAMOS Microautosampler are used and need to be programmed The experiment has the following features e NanoLC separation on a 75 um I D 15 cm PepMap column e Injection of 10 ul of cytochrom c sample 0 1 pmol yL e Sample loading onto a trap column for 5 minutes at a flow rate of 30 wL min pre concentration step e Solvent
40. ch as Sulfuric Acid and Nitric Acid swelling or attack by acid is not a problem with short flushing procedures Do not use PEEK tubing that is stressed bent or has a kink 13 Wear protective eye goggles when handling fused silica tubing i e installation cutting etc 14 If a buffer is used as a part of the mobile phase flush the system with several volumes of a methanol water 50 50 before it is shut down This will prevent salt buildup inside the unit 15 Do not use the instrument in ways other than those indicated in the instructions given in this manual x D850R3 User s Manual Switchos II CE DECLARATION OF CONFORMITY We LC Packings Nederland BV A Dionex Company Abberdaan 114 1046 AA Amsterdam The Netherlands declare that our product Switchos II Advanced Microcolumn Switching Unit is in confirmation with the following documents EEC directives 89 392 incl 91 368 and 93 44 machine safety and EEC directives 73 23 and 93 68 low voltage safety applied with the following standard EN61010 1 Safety requirements for laboratory equipment Class I Installation cat Il Pollution degree II LC Packings will not accept any liability for damages direct or indirect caused by connecting this instrument to devices which do not meet relevant WARNING Safety standards EEC directives 89 336 and 92 31 EMC requirements applied with the following standards EN 50081 1 Generic emission standard EN
41. circumstances shall LC Packings be liable for damage to persons or property This warranty is the only warranty given by LC Packings with respect to products and software provided with the products and is given in lieu of all other warranties express or implied including without limitation any warranty of merchantability or fitness for a particular purpose Your exclusive remedies and LC Packings s sole liability for any non conformity or defect in the products and such software will be those expressed herein Under no circumstances will LC Packings s liability arising from the performance or failure to perform of any product or software in contract in tort including negligence or otherwise exceed the purchase price of the product and software In no event will LC Packings be liable in contract in tort including negligence or otherwise for special incidental consequential or analogous damages including without limitation damages resulting from loss of use loss of profits loss of business or loss of goodwill even if LC Packings has been advised of the possibility of such damages This warranty comprises the entire warranty between LC Packings and the customer It overrides any warranty related language that may appear in the customer purchase order or other documentation provided by the customer This warranty shall be governed by and construed and enforced in accordance with the laws of the Netherlands It is non transfera
42. clude air freight drayage labor crating charges customs clearance charges etc The user should carefully follow the directions indicated on the Return Goods Instruction Sheet in the User s Manual After thirty days all transportation costs will be at the expense of the customer Software Warranty If at any time during the period ending ninety 90 days after delivery of any product to you you report and document any error in any software provided with such product and developed by LC Packings or any failure of any such software substantially to conform to LC Packings software description that limits or prevents use of the software by you we will use reasonable efforts to correct any such error or failure will replace such software or will terminate your license to use the software and refund the price of the related product In connection with any such termination and refund you will return the related product to LC Packings upon request The warranty will apply only to those portions of the software that were developed by LC Packings and that incorporated all program corrections and modifications if any delivered to you It will not apply to any error or failure due to machine error or to the misuse by or negligence of any person or entity other than LC Packings or to any software which is modified by any person or entity other than LC Packings User s Manual Switchos II D850R3 v Warranty Liability Under no
43. e made on the rear panel of the instrument FIGURE 2 1 SOLVENT SELECTION Button REMOTE LOCAL Switch He Inlet COMMUNICATION Connector INPUTS Connector Power Outlet to Loading Pump Main Power Switch Main Power Inlet aov aasa vA ASE 2A OW Fuse Loading Pump Power Switch Power Inlet EVENTS RS 232 1 Connector Connector RS 232 2 REMOTE Connector Connector FIGURE 2 1 Rear Panel of the Switchos Advanced Microcolumn Switching Unit caution Caution Avoid touching the electrical contacts on the terminal strips Electrostatic discharges could damage internal components The manufacturer will not accept any liability for damages directly or indirectly caused by connecting the Switchos Advanced Microcolumn Switching Unit to instruments which do not meet relevant safety standards User s Manual Switchos D850R3 2 3 Installation and Getting Started CAUTION 2 3 2 RS 232 Connectors The two RS 232 serial interfaces enable digital data transfer between the loading pump the UltiMate Micropump the UltiMate UV Detector if present and the PC These devices communicate with each other to form an integrated network To set up the RS 232 network connections e Connect the RS 232 1 connector of the Switchos loading pump the RS 232 2 connector of the UltiMate UV Detector and the COM port of the PC using the Y cable item a FIGURE 2 2 e Connect the RS 232 2 connector of the Switchos loading pum
44. e other approximately half a turn at a time and remove the screws d Remove the pump head Ie Note If the purpose of removing the pump head is to check the piston rods there is no need to disassemble the pump head any further Pull out each piston rod in a straight line using a pair of pliers To replace the Pump Head a Position the pump head onto the housing and carefully line up the screw holes Tighten all four set screws by hand b Alternating from one to the next tighten two diagonally opposed screws e g screws 2 and 3 half a turn at a time until the pump head is correctly seated onto the housing c Tighten the two other screws d Check that the screws that were tightened in step c are well tightened User s Manual Switchos II D850R3 6 5 Maintenance and Troubleshooting 6 3 4 Disassembling the Pump Head Replacing Piston Seals To disassemble the inert pump head titanium inlays a Remove the pump head and the piston rods Section 6 3 3 Detail of 13 Flow Orientation Mark Item Description P N Inert Pump Head 161399 1 Recessed Head Screw 2 Retaining Plate 3 Piston Rod 160047 4 Guide for Spring 5 Spring 6 Spacing Bolt 7 Support Washer 8 Piston Seal backflushing 161020 9 Pressure Plate 10 O Ring 160084 11 Piston Seal high pressure 161020 12 Titanium Inlay 161400 13 Check Valve Cartridge 160049 14 PE
45. eals should only be replaced when the pump leaks The useful lifetime depends on the mobile phase to be pumped and the back pressure of the system The typical lifetime of the seals is in the order of 2500 h It is recommended that both seals be replaced if one is defective g To remove the titanium inlays remove the four bushings the check valves and the PEEK adapters items 13 14 15 FIGURE 6 5 h Carefully insert new seals The spring side should be placed as indicated in FIGURE 6 5 To re assemble the pump head a Insert the titanium inlays the two check valve cartridges item 13 FIGURE 6 5 and the two PEEK adapters item 14 FIGURE 6 5 As you replace the check valves make certain that they are inserted in the right position caution Caution Make certain that the orientation mark of the check valve cartridges is placed so that the mark is on the bottom Installing the check valve cartridges in the wrong direction may lead to a damage of a cartridge or the pump head b Alternating from one to the next tighten the upper and the lower bushing half a turn at a time by hand c Replace the pressure plate with the 2 o rings items 9 10 FIGURE 6 5 and the two spacing bolts items 9 10 FIGURE 6 5 Alternating from one to the next tighten the two bolts by hand d Tighten the two bolts and then tighten the 4 bushings Make certain that all screws are securely tightened 6 3 5 Replacing the Check Valve Cartridge The
46. ected to valve B as shown in FIGURE C 1 The following steps are involved in the process a b c d Proteins are injected and trapped onto the ion exchange cartridge Valve A is placed in position 10 1 and non charged compounds e g detergents are flushed to waste Valve A is switched back into position 1 2 The ion exchange column is now in series with a protein digestion column and the reversed phase trap column mounted on valve B position 10 1 Proteins are eluted by injection of a salt solution The peptides are concentrated onto the reversed phase trap column Valve B is switched back from position 10 1 the 1 2 to place the trap column and separation column in series The gradient is started to elute the peptides from the trap column The Peptides are separated on the capillary nano column User s Manual Switchos II D850R3 C 1 Cleanup of Proteins a Column 2 Valve A presented in position 1 2 From Injection Valve Microflow Column 4 to UltiMate UV Detector Column i _ Waste 3 ValveB 9 presented 4 inposition 8 10 1 7 6 Microflow from UltiMate Waste FIGURE C 1 Fluidic Setup of the two 10 Port Valves TABLE 1 Description of the Columns Column No Column Type 1 lon exchange 2 Protein Digestion 3 Trap Column 4 Separation Column C 2 D850R3 User s Manual Switchos II APPENDIX D Connecting Fused Silica Capillary CAUTION D 1 Ove
47. ection valve Additionally it reads the COMMUNICATION port and reads out the status of the push buttons switches and the remote control input connector e High Pressure Filter serves to remove particulate matter from the mobile phase ae Note For highest performance complete automation and ease of operation the Switchos Il Advanced Microcolumn Switching Unit should be used in combination with the LC Packings UltiMate Capillary HPLC system and the FAMOS Microautosampler These units can be readily connected via a local area network Chapter 2 3 for secure communication between the various modules User s Manual Switchos II D850R3 1 3 Introduction 1 3 Front View of Switchos Il The front view of the Switchos Il Advanced Microcolumn Switching Unit is presented in FIGURE 1 2 ON STANDBY Switch He Regulating Valve LEDs Solvent Swithos Selection Valve by LC Packings LEDs Valve A Valve A Button with LED Valve A LEDs Valve B Valve B Microflow Outlet Button with LED Valve B Loading Pump eodd eyboar Backflushing Connections Pump Head Purge Screw High Pressure Se Inlet Filter Q FIGURE 1 2 Front View of the Switchos II Advanced Microcolumn Switching Unit 1 4 D850R3 User s Manual Switchos II Introduction 1 4 Rear View of Switchos Il The rear view of the Switchos II Advanced Microcolumn Switching Unit is presented in FIGURE 1 3 SOLVENT SELECTION Butto
48. er y Fluidics Fusica Nano Series Pico Series uy Guard y Precolumn Nano Precolumn Dionex is a Trademark of Dionex Corporation CHROMELEON is a Trademark of Dionex Corporation PEEK polymer is a Trademark of Victrex plc Teflon is a Registered Trademark of E I duPont de Nemours and Company Windows and Microsoft are Registered Trademarks of Microsoft Corporation VICI and Cheminert are Registered Trademarks of VICI Valco Instruments Co Inc Other product names or company names mentioned herein may be the trademarks of their respective owners Printed in the Netherlands D850R3 Table of Contents WARRANTY v INSTRUCTIONS FOR RETURNING INSTRUMENTS vii WARNINGS AND SAFETY PRECAUTIONS ix CE DECLARATION OF CONFORMITY xi CHAPTER 1 Introduction 1 1 1 1 Features of the Switchos II Advanced Microcolumn Switching Unit 1 1 1 2 General Design of the Switchos Il Advanced Microcolumn Switching Unit 1 2 1 3 Front View of Switchos II 1 4 1 4 Rear View of Switchos II 1 5 1 5 Contents of this Manual 1 6 1 6 For Additional Information 1 7 CHAPTER 2 Installation and Getting Started 2 1 2 1 Installation 2 1 2 1 1 Location of Switchos Il in the Laboratory 2 1 2 2 Unpacking 2 2 2 3 Installing the Switchos II with the U tiMate Capillary HPLC System and the FAMOS Microautosampler 2 3 2 3 1 Electrical Connections 2 3 2 3 2 RS 232 Connectors 2 4 2 3 3 COMMUNICATION Connector 2 4 2 3 4 INPUTS Connector 2 5 2 3 5 Tr
49. etermine the number of available controls and their functionality via the design tools depending on your individual requirements refer to the CHROMELEON user s manual for more detailed information A number of standard panels for the most commonly used UltiMate configurations are available Refer to TABLE 4 3 and choose the panel which corresponds to your instrument configuration and then load this panel from the Dionex Templates Panels Dionex LC directory TABLE 4 3 Panel Name vs Instrument Configuration Control Panel Name Instrument Configuration Ultimate pan UltiMate Ultimate FAMOS pan UltiMate and FAMOS UltiMate FAMOS Switchos II Ultimate FAMOS_Switchos pan Switchos II valves are controlled by the Loading Pump Section 4 2 3 UltiMate FAMOS Switchos II Ultimate FAMOS_ Switchosll pan Switchos Valves are controlled by serial communication Section 4 2 2 Depending on your authorization you can create a completely new control panel In order to create or change properties of a control panel change to the Layout Mode on the Edit menu A new control panel is saved as a PAN file pan and is then available to the user refer to the CHROMELEON user s manual for more detailed information 4 3 3 Connecting the Control Panel to a Timebase A panel needs to be connected to a certain Timebase to allow the control of the instruments of this Timebase To connect the control panel to a
50. g Flow Rate 3 5 Maximum Pressure 3 5 Signal Names 4 5 Side Panels Removing 6 8 Solvent Bottles 1 3 Solvent Bottle Caps Testing 5 2 Solvent Lines Testing 5 3 Solvent Selection Valve 1 2 3 3 Testing 5 2 Sparging Filter Frit Replacing 6 3 Sparging the Mobile Phase 2 10 Spare Parts 6 13 A 3 D 4 Inert Version 6 13 A 3 D 4 Specifications 7 1 SSV Valve 1 2 3 3 Standby 3 2 Starting Flow Delivery 3 6 4 8 Stopping Flow Delivery 3 6 Switching Valves 1 2 A 1 Assembly A 2 Disassembly A 2 Maintenance A 2 Spare Parts A 3 Testing 5 4 System Parameter Typical 6 13 T Testing 5 1 Timebase 4 7 Triggering a Mass Spectrometer 2 5 Troubleshooting 6 11 Loading Pump 6 11 Switching Vales 6 12 Typical System Parameters 6 13 U Unpacking 2 2 User Interface 3 1 Vv Valve A and B Controls 3 3 Virtual Channel Driver 4 5 l 2 D850R3 User s Manual Switchos II
51. g a Nano Column D 1 Spare Parts 6 13 A 3 D 4 Pump Head 6 6 Instrument Configuration 4 7 INPUTS Connector 2 5 With Other HPLC Systems 2 7 Using the Event Outputs and 2 6 Integrated Valve Controller 1 3 Installation With UltiMate Capillary HPLC and FAMOS Microautosampler 2 3 With Other HPLC Systems 2 7 Connection 2 9 Degassing System 1 3 5 2 Regulating Valve 3 3 High Pressure Filter 1 3 Replacing 6 4 L LEDs 3 3 Loading Pump 1 3 3 5 Options 4 9 Removal 6 9 LOCAL Mode 3 4 Location in Laboratory 2 1 M Maintenance 6 2 A 2 Maximum Pressure Setting 3 5 Mobile Phase Considerations 2 14 N Nano Column Connecting D 1 Network Identification B 1 O ON Standby Switch 3 2 P Piston Seals Replacement 6 6 Power Connector With UltiMate Capillary HPLC and FAMOS Microautosampler 2 3 With Other HPLC Systems 2 7 Powering up the System 3 2 Program User s Manual Switchos II D850R3 Index Creating 4 8 Using the Wizard 4 8 Pump Head Disassembly 6 6 Inert 6 6 Purging 3 6 2 11 Pump Options 4 10 Loading pump 4 9 Purging the Loading Pump 2 11 3 6 R Rear View 1 5 REMOTE Mode 3 4 Relay 2 7 Tab 4 4 Removing Loading Pump 6 9 Side Panels 6 8 Pump Head 6 5 Replacing Check Valve Cartridge 6 7 Fuses 6 7 High Pressure Filter 6 4 Sparging Filter Frit 6 3 Piston Seals 6 6 RS 232 Connectors 2 4 Control 4 3 S Sample Considerations 2 14 Seals Replacement 6 6 Server 4 2 Configuration 4 2 Settin
52. ges Note Please refer to our web site for more detailed information on stationary phases and column dimensions available www lcpackings com 6 5 6 Pump Head and Tubing Inert Pump Head Titanium Inlays Part Number Description 161399 Inert Pump Head Titanium Inlays 160049 Check Valve Cartridge 1 piece 160050 Check Valve Cartridges set of 5 User s Manual Switchos II D850R3 6 15 Maintenance and Troubleshooting Piston Seals Graphite Fibre Filled PTFE TaT 1 4 O D and 0 315 O D set of 4 ka 160047 Sapphire Piston 161400 Titanium Inlay for the Inert Pump Head 162300 PEEK Adapter for inert Pump Head 160084 O Ring 17 mm x 1 5 mm 161026 Torque Wrench Connecting Tubing for the Switchos Inert Version Connecting Tubing Pressure Sensor to High Pressure Filter Titanium 1 Connecting Tubing for Pump Head 198000 Titanium 2 poe 161022 Connecting Tubing Pump Outlet to i Pressure Sensor Titanium 3 160053 PEEK Connecting Tubing Pump Inlet 4 Connecting Tubing for the Switchos Standard Version 161024 161023 Connecting Tubing Pressure Sensor to High Pressure Filter Stainless Steel 1 Connecting Tubing for Pump Head Stainless Steel 2 162009 Connecting Tubing Pump Outlet to Pressure Sensor Stainless Steel 3 3 160053 PEEK Connecting Tubing P
53. gradient from O 50 B in 30 minutes e Analysis time of 60 minutes e Recording of the UV signal at 214 nm the column pressure the trap column pressure and the pump pressure CHROMELEON automatically provides a program wizard when a new program is to be created which guides through creation of the program file To create a new program using the Program Wizard a Select the New command on the File menu and then select Program File from the New box The Program Wizard starts automatically b Select the Timebase from the Se ect Timebase Options box c Setup the column oven temperature if applicable in the Oven Options box d Setup the trap column pressure limits of the Switchos II Loading Pump e g O bar and 200 bar and fill in the time table in the Loading Pump Options box Program Wizard Loading_Pump Options x Trap Column Pressure Limits Lower Limit 0 0 0 0 400 0 bar Upper Limit 200 0 0 0 400 0 bar m Lime Program No Time mini Flow mLminl Ssv vale vanes _AppendLie 0 000 0 030 T ey EE 5 000 0 030 a 104 12 Insert Line a 40 000 ooj A 12 12 lt Back Cancel Help FIGURE 4 9 The Loading Pump Options Box e Setup the gradient conditions in the Pump Options box use the Multi step Gradient option to run a gradient User s Manual Switchos II D850R3 4 9 Software Control f Define the gradient in the Flow Gradient Options box FIGURE 4 10
54. he pressure is similar to what was observed when the system was last used 2 6 4 Setting up the Fluidic Connections The Switchos is used in a broad range of applications and the user can configure the unit to meet the specific needs of the laboratory In this section we show the fluidic setup of a pre concentration application using the Switchos Advanced Microcolumn Switching Unit in conjunction with the FAMOS Microautosampler and the UltiMate Capillary HPLC System FIGURE 2 12 Additional examples are presented in Appendix C While these examples may not meet the specific needs of the analyst it is likely that they can be used with minor modification Pre Concentration using the Switchos and the FAMOS Microautosampler FAMOS Analytical microcolumn port 6 Micro flow to UltiMate e Switchos Vav TE gt 3 ValveA 9 Trap column presented 4 in position 8 FAMOS ne port 1 5 6 ian Micro flow D 600000 from UltiMate SSE EE o O ON Waste FIGURE 2 12 Typical Pre Concentration Setup Loading the Trap Column To setup the Switchos for the pre concentration application presented in FIGURE 2 12 connect the instruments as follows a Connect port 1 of the injection valve of the FAMOS Microautosampler to the Nano Micro flow outlet of the Switchos loading pump using the 130 um I D PEEK tubing provided with the instrument D850R3 User
55. hort moment it moves back to channel A REMOTE control mode The Switchos is in REMOTE control mode e g the LEDs in the push buttons are not illuminated The signal applied to the SSV input has been active for longer 1 5 sec Change control mode to LOCAL REMOTE LOCAL switch on the rear panel Apply a shorter signal lt 1 5 seconds The valve A B switches to position 1 2 as soon as the instrument is set into REMOTE control mode At the present time there is no active input signal and the valve is forced into position 1 2 Change the setting of the instrument controlling Switchos according to the needs of the application LEDs 1 2 and 10 1 of Valve A B are switched off or switched on at the same time The Switching Valve A B does not switch Switch OFF the instrument and ON again to reset the error due to valve sticking Clean Valve clamp ring slippage Tighten clamp ring other positioning problems Contact LC Packings LED A D illuminated at the same time The Solvent Selection Valve does not switch Switch OFF the instrument and ON again to reset the error due to valve sticking Contact LC Packings clamp ring slippage Contact LC Packings other positioning problem Contact LC Packings IES Note It is important to note that many analytical problems are be caused by external influences such as the temperature and or
56. ia the COMMUNICATION port Section 2 3 3 Refer to Sections 2 4 and 2 5 if the valves need to be controlled by digital input signals e g the instrument is to be installed in conjunction with a software package which does not support the serial communication 2 3 5 Triggering a Mass Spectrometer or an Other Device If you want to provide a mass spectrometer or other external device with a trigger signal from the CHROMELEON software at some event e g on the beginning of the gradient program connect the appropriate input of the mass spectrometer or other device to the EVENT 8 output relay contact of the UltiMate Micropump as shown in FIGURE 2 3 UltiMate Micropump or EVENT 8 other device Relay FIGURE 2 3 Cable to trigger the MS or another Device by EVENT 8 Mass Spectrometer 2 3 6 Power Connector Since the Switchos is fitted with a universal power supply for input voltages from 90 to 260 V manual setting of the supply voltage is not required The power cord should be inserted in the socket directly below the Main Power switch on the right side of the rear panel FIGURE 2 1 In addition the loading pump should be plugged into the socket above the Main Power switch DANGER Danger The Switchos must be connected to a power source that is connected to a true ground User s Manual Switchos D850R3 2 5 Installation and Getting Started 2 4 Using the Event Outputs or the FAMOS Auxiliaries
57. ife gt warNninc Warning The information in a warning statement relates to a procedure practice condition or action that if not done correctly or adhered to could lead to severe injury and or damage or destruction to parts or all of the equipment gt cauTION Caution The information in a caution statement relates to a condition that could lead to damage to equipment and or lead to invalid analytical results 4 Note The information in a note statement relates to important information that should be read and understood before continuing Safety Precautions ae Note The following precautions should be followed to minimize the possibility of personal injury and or damage to property Ie Note Make certain that you are familiar with the contents of this manual before working on the system 1 The system should be installed in a well ventilated laboratory If the mobile phase includes volatile or flammable solvents make certain that they are not allowed to enter the workspace 2 If the mobile phase includes volatile or flammable solvents avoid open flames and sparks 3 Ifa leak occurs turn off power to the instrument and remedy the situation immediately 4 All components of the system should be plugged into a common power line that is directly connected to a true ground 5 When the panels are removed dangerous electrical connections will be exposed Disconnect the instrument from all power sources before removi
58. iggering a Mass Spectrometer or Other Device 2 5 2 3 6 Power Connector 2 6 2 4 Using the Event Outputs or the FAMOS Auxiliaries 2 6 2 5 Installing the Switchos with Other HPLC Systems 2 7 2 5 1 INPUTS Connector 2 7 2 5 2 Power Connector 2 8 2 6 Fluidic Connections Switchos Il to the HPLC System 2 9 2 6 1 Preliminary Operations 2 9 2 6 2 He Connection 2 9 2 6 3 Sparging the Mobile Phase and Flushing the Solvent Lines 2 10 2 6 4 Setting up the Fluidics Connections 2 12 2 7 Routine Operation of the System 2 14 2 7 1 Sample and Mobile Phase Considerations 2 14 CHAPTER 3 The User Interface 3 7 3 1 Overview 3 1 3 2 Powering up the Switchos II 3 2 3 3 Controls on the Front and Rear Panel 3 3 3 4 LOCAL Mode vs REMOTE Mode 3 4 3 5 Basic Operations for the Loading Pump 3 5 User s Manual Switchos II D850R3 i Table of Contents 3 5 1 Setting the Flow Rate 3 5 3 5 2 Setting the Maximum Pressure 3 5 3 5 3 Starting and Stopping Flow Delivery 3 6 3 5 4 Purging the Loading Pump 3 6 CHAPTER 4 CHROMELEON Control 4 1 4 1 Overview 4 1 4 2 Server Configuration 4 2 4 2 1 Adding and Configuring the Switchos 4 2 4 2 2 Switchos II controlled by a RS 232 COM Port 4 3 4 2 3 Switchos Il controlled by Event Outputs 4 4 4 2 4 Adding and Configuring the Virtual Channel Driver 4 4 4 3 UltiMate Event Output Programming 4 7 4 3 1 Starting CHROMELEON 4 7 4 3 2 The Control Panel 4 7 4 3 3 Connecting the Control Panel to a Timebase 4 7 4 3 4 Starting the Flow
59. ight nut and the ferrule onto the sleeve as shown in FIGURE D 1 Insert this assembly into one of the fitting ports of the supplied assembly tool P N160142 screwing the nut in two or three turns by hand Push the sleeve and the Nano column capillary all the way forward into the fitting port that the sleeve and the capillary seats firmly Ss Note It is essential that the sleeve and the NANO column capillary are inserted all the way forward into the union for a proper zero dead volume connection d Manually turn the nut into the union until it is finger tight FIGURE D 2 Attaching a PEEK Sleeve Connector to a NANO Column e Carefully turn the nut 1 4 turn 90 past the point where the ferrule starts to grab the sleeve FIGURE D 2 SF note The amount of force to turn the nut can vary considerably due to the friction between the nut and the threads and as well as the composition and wall thickness of the tubing used Because of these variables we do not provide a torque specification f Remove the fitting and inspect it When you pull gently at the NANO column capillary you should not be able to move the column capillary in the sleeve e g it should be grabbed by the sleeve FIGURE D 3 If the sleeve moves laterally reinstall the fitting into the fitting port and tighten it another 1 8 turn past finger tight FIGURE D 3 PEEK Sleeve Connector attached to a Nano Column D 2 D850R3 User s Manual Sw
60. ilter should be replaced To replace the high pressure in line filter e Remove the capillary item 1 FIGURE 6 2 between the purge valve and the filter unit d EJ FIGURE 6 2 High Pressure Inline Filter f Use the socket driver P N 160073 or a wrench 11 mm to remove the filter holder item 2 FIGURE 6 2 g Remove the filter and place a new filter into the holder FIGURE 6 3 Filter Description P N Filter Standard color black 160072 Inert color blue 161107 Filter Frit Holder Standard stainless steel 163021 Inert titanium 162108 FIGURE 6 3 In Line Filter in Filter Holder h Re mount the filter holder caution Caution Make certain that the filter remains in the filter holder when screwing the holder in and do not overtighten the filter holder Overtightening could result in distortion of the filter which may lead to leakage and or premature failure of the component 6 4 D850R3 User s Manual Switchos II Maintenance and Troubleshooting 6 3 3 Removing the Pump Head To remove the Pump Head a Remove the inlet and the connecting capillary FIGURE 6 4 FIGURE 6 4 Disassembly of the Pump Head b Loosen two diagonally opposed screws e g screws 2 and 4 in FIGURE 6 4 by a quarter turn at a time and remove the screws c Carefully loosen the two remaining screws e g screws 3 and 5 in FIGURE 6 4 alternating from one to th
61. inert pump head is equipped with two check valves item 13 FIGURE 6 5 one on the inlet side of the main piston lower right and on the outlet of the main piston upper right while two PEEK adapters are used on the auxiliary piston lower and upper left side User s Manual Switchos II D850R3 6 7 Maintenance and Troubleshooting To remove clean the check valves cartridge s a Unscrew the two check valve bushings items 15 FIGURE 6 5 and remove the check valve cartridges b As you replace the check valves make certain that they are inserted in the right position item 13 FIGURE 6 5 Tighten the two check valve bushings by hand c Alternating from one to the next tighten the upper and the lower bushing half a turn at a time d Tighten the two check valve bushings caution Caution Make certain that the orientation mark on each check valve is placed so that the mark is on the bottom Installing the check valves in the wrong direction may lead to a damage of the pump head 6 3 6 Removing the Side Panels The side panels must be removed to gain access to remove the loading pump and to access the Solvent Selection Valve To remove the panels a Remove three screws on the back of each side panel items 1 6 FIGURE 6 6 FIGURE 6 6 Rear View of Switchos b Remove the side panels 6 8 D850R3 User s Manual Switchos II Maintenance and Troubleshooting 6 3 7 Removing the Loading Pump To
62. ion and Getting Started DANGER CAUTION CAUTION CAUTION e f flammable or toxic solvents are to be used a suitable ventilation system should be provided e The use of open flames in the laboratory should be prohibited e Corrosive vapors or dust should not be present as these materials can adversely affect the long term performance of the system The Switchos Advanced Microcolumn Switching Unit requires approximately 190 mm 7 5 of linear bench space The lab bench should be capable of supporting the entire system for the LC Packings UltiMate FAMOS and Switchos we recommend that the lab bench be capable of supporting at least 100kg 225 Ib The power consumption of the Switchos is 100 VA the power consumption of the UltiMate Capillary HPLC System is 250 VA and of the FAMOS Microautosampler is 250 VA Danger The Switchos Advanced Microcolumn Switching Unit must be connected to a power source that is connected to a true ground In addition all other components of the system e g the HPLC pump the detector should be connected to the same ground Caution Do not install the Switchos Advanced Microcolumn Switching Unit in areas subject to shock dust or in direct sunlight 2 2 Unpacking When the Switchos Advanced Microcolumn Switching Unit is received carefully unpack the unit and verify receipt of all components according to the packing list some components include sub packing lists It is recommended that
63. ion rate in the UV Options box i Define the type of injection you want to use and the corresponding injection parameters in the Sampler Options box 4 10 D850R3 User s Manual Switchos II Software Control Program Wizard Sampler Options Paa FIGURE 3 12 The Sampler Options Box Ie Note If you want to create a User Defined Program please refer to the CHROMELEON online F1 key help more details j Select the number of UV channels used and the data acquisition time in the Acquisition Options box FIGURE 4 13 Program Wizard Acquisition Options FIGURE 4 13 The Acquisition Option Box The number of UV_VIS_n Options boxes to be filled in is dependent on the number of channels that you are monitoring e g the different wavelengths FIGURE 4 14 User s Manual Switchos II D850R3 Software Control Program Wizard UY_VIS_1 Options FIGURE 4 14 The UV_VIS_n Options Box k Specify the program name and path Note The Appendix E of the user s manual of the UltiMate system presents 4 additional examples 4 3 7 Changing a Program File A CHROMELEON program can easily be changed by a double click on the program name in the CHROMELEON browser The program appears in a text format Program lines are inserted by describing the parameter or by using the F8 command D850R3 User s Manual Switchos II CHAPTER 5 Testing the Switchos 5 1 Overview This chapter describes a se
64. iscusses how to control the Switchos II Advanced Microcolumn Switching Unit by the CHROMELEON software and how to setup the software modules Chapter 5 Testing the Switchos II describes a series of operations to determine that the unit is functioning in an acceptable manner Chapter 6 Maintenance and Troubleshooting describes a variety of maintenance procedures to optimize the performance of the microcolumn switching unit In addition it discusses how the operator can determine the cause of a difficulty in the operation of the instrument and includes a list of spare replacement parts Chapter 7 Specifications presents the specifications of the Switchos II Advanced Microcolumn Switching Unit In addition a series of appendices that provide information about the valves that are incorporated in the unit interfacing the unit to the local area network and typical applications Appendix A Switching Valves describes the valves that are included in the Switchos Il Advanced Microcolumn Switching Unit In addition it presents information about the disassembly and reassembly of the valve Appendix B Network Identification provides explains how the Switchos II is identified on the system network so that it can communicate with other components Appendix C Cleanup of Proteins presents an explanation of how the Switchos II is used for sample clean up protein digestion and peptide separation Appendix D Connecting Fused Silica Capillary ex
65. itchos II Modifications CAUTION g Remove re inspect and repeat if necessary FIGURE D 4 Installing the Pre assembled Fitting h Install the pre assembled fitting in the inert PAEK injection switching valve and manually turn the nut into the valve until it is finger tight FIGURE D 4 D 3 Using Long PEEK Hex Style Nuts In case the supplied fingertight fittings cannot be used due to limited space use the long PEEK hex style nuts P N 161007 supplied with the instruments instead FIGURE D 5 A FIGURE D 5 The Long PEEK Hex Style Nut To use the supplied long hex nuts a Assemble the fitting as described in Section D 2 b Install the pre assembled fitting in the inert PAEK injection switching valve and manually turn the nut into the inert PAEK valve until it is finger tight FIGURE D 4 c Carefully tighten the hex style nut using a wrench Caution Never use the inert PAEK injection switching valves to pre assemble a fitting and never use any tool to tighten the fingertight nut This may lead to damage of the inert PAEK valve User s Manual Switchos II D850R3 D 3 Modifications D 4 Spare Parts Lists Part Number Description Figure 160142 Tool for pre assembling of INERT fittings zE 160492 PEEK sleeves precision cut and polished for SS connections with fused silica tubing 300 um O D set of 5 b sgt Wl a we 161007 PEEK 1 16 Universal Fitting for
66. itchos Pump Loading Pump T Inject Valve via START IN of UM Detector Iniectvave Cancel Epp Help FIGURE 4 2 The UltiMate Configuration Box Components Tab 4 2 D850R3 User s Manual Switchos II Software Control c Make sure that the SwitchosPump box is checked on the Components tab The valves of the Switchos II can either be controlled directly via the COMMUNICATION connector and via a free COM port of the PC Section 2 3 or by the event outputs of the Switchos II Loading Pump via the INPUTS connector Section 2 4 4 2 2 Switchos Il controlled by a RS 232 COM Port To control the unit by serial communication a Add the LC Packings Switchos II SSV device to the Timebase FIGURE 4 1 This will present the LC Packings Switchos II SSV configuration box FIGURE 4 3 LC Packings Switchos IISS V SSV Valve Valve_B Error Levels Device Name 5S Serial Communication Pott COM2 hes Baudrate 9600 Parity None Data Bits 8 Stop Bits 1 Handshake Off FIGURE 4 3 The Switchos Il Configuration Box Select the COM port which controls the unit and click OK to confirm the setting To verify or change the configuration double click on the LC Packings Switchos II SSV device item 2 FIGURE 4 1 IES Note If there are not enough COM ports available the valves of the Switchos Il can be controlled by the event outputs of the Loading Pump Section 4 2 3 User s
67. lation and Getting Started CAUTION CAUTION 2 5 Installing the Switchos with Other HPLC Systems The electrical connections that are required depend on the nature of the instrumentation and the desired application In this section we provide general information about how the instrument can be interfaced to equipment from other manufacturers The user should also refer to the documentation provided with this equipment The Switchos is controlled via the INPUTS connector on the rear panel and the keypad of the loading pump Caution If you are interfacing the Switchos to instrumentation from other manufacturers ensure that input voltages and output voltages are within the ranges indicated in the specifications Chapter 7 If you have any questions please call LC Packings The LC Packings warranty will not be valid if the Switchos is damaged due to interfacing of the system to instrumentation from third parties 2 5 1 INPUTS Connector The positions of the switching valves A and B and the Solvent Selection Valve SSV are controlled by the INPUTS connector FIGURE 2 6 Contact closure relay TTL and open collector outputs can drive the Switchos inputs Caution Check to make certain that the maximum ratings of the outputs matches with the requirements of the Switchos Advanced Microcolumn Switching Unit Chapter 7 PIN SIGNAL INPUTS 1 VALVE A 2 GROUND O22 00000 4 VALVE B 0000000 7 SSV 8 GROUND
68. le Edit View Server Help ar i Ga 2 S BD PM 3 v E amp esquire pc Sharable Devices Messages Server ESQUIRE PC Expert Serer ESQUIRE PC connected 08 34 22 CmServer Chromeleon Server Version 6 50 Build 943 Now 12 2002 Copyright c 1994 2002 Dionex Corporation All rights reserved 08 34 23 Chromeleon Default USB Driver Version 6 50 Build 943 Copyright c 1994 2002 Dionex Corporation All rights reserved 08 34 23 UltiMate Chromeleon LC Packings UltiMate Driver Version 6 50 SP1 Build 947 RC 1 Copyright c 1994 2002 Dionex Corporation All rights reserved Fp 03 34 23 SER_IO Dionex Serial Interface Driver Version 6 50 Build 943 Copyright o 1994 2002 Dionex Corporation All rights reserved F 08 34 23 Famos Autosampler Driver Version 6 50 Build 943 Copyright o 1994 2002 Dionex Corporation All rights reserved 08 34 23 VirtChan Chromeleon Virtual Channels Version 6 50 Build 943 Copyright c 1994 2002 Dionex Corporation All rights reserved F 08 34 23 GenStd Chromeleon Serial Generic Driver Version 6 50 Build 943 Copyright c 1994 2002 Dionex Corporation All rights reserved F 02 34 23 Initializing driver LC Packings Switchos II SSV F 02 34 23 Initializing driver Virtual Channel Driver 08 34 23 Initializing driver LC Packings F amos 993 34 23 Initializing driver LC Packings UltiMate Switchos 03 34 23 Initializing driver Dionex Default USB F 11 54 08 User Administrator from ESQ
69. lease include the following a Company letterhead with the following information Your Name Complete Mailing Address Telephone Number fax number and e mail address Return Material Authorization RMA Number A detailed description of the problem SAR E A ee oe The name of the LC Packings personnel to whom you have spoken to regarding the problem 7 Return Shipping Information if appropriate b Relevant chromatograms c A purchase order if the system is not in warranty Ie Note The completed and signed HEALTH AND SAFETY form must be returned to LC Packings service department fax or mail prior to the return of any component or attached outside the shipping package In addition the provided RMA number must be clearly marked on the outside of the shipping package Failure to complete and return this form will result in the package returned without the parts being inspected or credit issued User s Manual Switchos Il D850R3 vii Instructions for Returning Instruments This page intentionally left blank viii D850R3 User s Manual Switchos II Warnings The Danger sign Warning sign and the Caution sign shown below are included in various locations in this manual These signs provide the following information gt DANGER Danger The information in a danger statement relates to a procedure practice condition or action that if not done correctly or adhered to could lead to personal injury or loss of l
70. mp for the lists of spare parts for the Micropump 6 5 1 Major Items Part Number Description 163662 Switchos Microcolumn Switching Module consists of 1x loading 160170 pump 2x 10 port valves and 1x solvent selection 163663 Switchos Microcolumn Switching Module INERT Version consists of 1x loading pump 2x 10 port valves and 1x solvent selection valve a Instrument equipped with a blue LC Display b Instrument equipped with a green yellow LC Display 160559 Notes 6 5 2 Tubing Fittings and Bottles Part Number Description 160180 130 um I D x 50 cm PEEK tubing set of 2 pieces 160181 130 um I D x 100 cm PEEK tubing set of 2 pieces 160174 250 ml safety solvent bottle 160175 Caps for solvent bottles set of 6 pieces 162015 Bottle cap assembly 160183 3 meter Helium supply tubing 6 mm O D 161470 T piece for Helium tubing 160483 Capillary cutter 160173 1 16 universal fingertight fitting one piece design extra long body 4 pieces 160492 PEEK sleeves precision cut and polished for connections with fused silica tubing 300 um O D set of 5 pieces 160266 Set of 20 nuts and ferrules for Valco switching valves PEEK Fingertight nuts and ferrules for inert injection switching 161000 valves set of 10 161007 ae Hex nuts and ferrules for inert injection switching valves set 163915 PEEK ferrules with groove for inert valves 20 pcs Capillary LC Standard
71. n REMOTE LOCAL Switch SOAT PANE SELECTION LOCAL He Inlet COMMUNICATION Connector INPUTS Connector WARNING MAKE SURE THAT AIR CIRCULATION WILL NOT BE IMPEDED Power Outlet to Loading Pump Risk OF ELECTRIC SHOCK Main Power Switch DISCONNE CT POWER BEFORE SERVICING sose0v 4743 Main Power Inlet CE a o vA FUSE 2A SLOW Fuse Loading Pump Power Switch Power Inlet EVENTS RS 232 1 Connector Connector RS 232 2 REMOTE Connector Connector FIGURE 1 3 Rear View of the Switchos Il Advanced Microcolumn Switching Unit User s Manual Switchos II D850R3 1 5 Introduction 1 5 Contents of this Manual Note This manual covers the standard version of the Switchos Il Advanced Microcolumn Switching Unit as well as the inert version If you are using an inert version please refer to Appendix D which includes important information about how to connect fused silica tubing to the inert switching valves This manual includes the following information Chapter 2 Installation and Getting Started describes how to install the Switchos Il Advanced Microcolumn Switching Unit Chapter 3 The User Interface describes the system controls explains how the unit is used in manual mode and provides a short overview of the micropump controls A detailed discussion of the micropump is presented in the UltiMate Micropump User s Manual which is supplied with the system Chapter 4 Software Control d
72. ng the panels 6 Always replace blown fuses with fuses of the same size and rating indicated on the fuse holder and panel Refer to Section 6 3 6 of this manual for more information on Fuses User s Manual Switchos II D850R3 ix Warnings and Safety Precautions 7 Repair or replace faulty power cords and all communication cables 8 Many organic solvents and buffers are toxic Make certain that you know the toxicological properties of all mobile phases that you are using 9 The toxicological properties of many samples may not be well known If you have any doubt about a sample treat it as if it contained a potentially harmful substance 10 Wear protective eye goggles when handling mobile phases or operating the instrument An eye wash facility and a sink should be close to the unit If any mobile phase splash on the eyes or skin wash the affected area and seek medical attention 11 Dispose of all waste mobile phase in an environmentally safe manner that is consistent with all local regulations Do not allow flammable and or toxic solvents to accumulate Follow a regulated approved waste disposal program Never dispose flammable and or toxic solvents through the municipal sewage system 12 PEEK tubing is used in a variety of locations While this polymer has superb chemical resistance to most organic solvents it tends to swell when it is contact with CHCls DMSO and THF In addition it is attacked by concentrated acids su
73. og loading_pump t vV OvenTemperatue degC 1 00 Analog Oven Temperat TrayT emperature deg 1 00 Analog Sampler Tempe VirtualChannel_06 1 00 Analog VirtualChannel NZ 10n Analan P Ej je Deactivate check boxes to remove unused channels from Online Change FIGURE 4 7 Example for a typical Virtual Channel Setup eS Note Make sure to use the same signal names and the same formulas as indicated in TABLE 4 2 If you modify the signal names or the formulas they may not be recognized by the predefined panels and some functions may not work properly 4 6 D850R3 User s Manual Switchos II Software Control 4 3 Using CHROMELEON The following section provides information how to use the Switchos II Advanced Microcolumn Switching Unit in conjunction with the CHROMELEON software the LC Packings UltiMate system and the FAMOS Microautosampler In addition to the steps used to program the Switchos basic information is provided how to control the other modules 4 3 1 Starting CHROMELEON Click on the CHROMELEON Server icon to start the CHROMELEON server which is the interface between the CHROMELEON user interface and the instruments Click on the CHROMELEON icon to start the software 4 3 2 The Control Panel The Control Panel abbreviated the panel controls and monitors the instruments of one Timebase With regard to appearance and function it is a special type of window You can d
74. ont panel of the pump FIGURE 3 5 4 5 Es e aro E QOOOO_ E TEO i START STOP Purge START STOP Pump Status of Pump Flow Rate Pressure Setup Menu Access GLP Menu Access value for solvents not used Arrow Keys for Cursor 10 Data Input Keys OANDARWNH FIGURE 3 5 The Front Panel of the Loading Pump 3 5 1 Setting the Flow Rate To set the flow rate use the arrow keys to move the cursor to the Flow field item 4 FIGURE 3 5 and enter the desired flow rate 3 5 2 Setting the Maximum Pressure The maximum pressure value for the pump can be set via the Pressure field item 5 FIGURE 3 5 As an alternative it can be set via the PRESSURE LIMITS screen of the SETUP Menu global setting see UltiMate Micropump User s Manual To set the maximum pressure move the cursor to the Pressure field item 5 FIGURE 3 5 and enter the desired value When this field is being edited it is bracketed by a pair of vertical lines to indicate that this is a programmed value rather than the actual pressure During operation of the pump the actual pressure is indicated However if the cursor is moved to the Pressure field during operation the maximum pressure value will be indicated and the brackets will be presented User s Manual Switchos D850R3 3 5 The User Interface CAUTION 3 5 3 Starting and Stopping Flow Delivery When the desired parameters have been entered the flow delivery can be star
75. osition of the corresponding valve e LEDs Valve A indicate the position of valve A When Valve A is switched into position 1 2 e g the fluidic connection is between inlet 1 and inlet 2 the upper LED 1 2 is illuminated e LEDs Valve B indicate the position of valve B When valve B is switched into position 1 2 e g the fluidic connection is between inlet 1 and inlet 2 the upper LED 1 2 is illuminated e LEDs Solvent Selection Valve indicate the solvent bottle from which solvent is being withdrawn at the present time e He Regulating Valve used to control the flow of He for sparging see Section 2 5 User s Manual Switchos D850R3 3 3 The User Interface The upper rear panel of the Switchos FIGURE 3 4 includes the following ery SOLVENT SELECTION Button REMOTE LOCAL Switch FIGURE 3 4 Control Elements on the Upper Rear Panel e SSV Solvent Selection Valves Button pressing the button will step to the next solvent channel from A to B B to C and finally from D back to A e LOCAL REMOTE Switch used to select the control mode 3 4 LOCAL Mode vs Remote MODE The control mode of the Switchos Advanced Microcolumn Switching Unit can be set via the REMOTE LOCAL switch on the rear panel FIGURE 3 4 LOCAL mode when the REMOTE LOCAL switch is set to LOCAL mode the switch is set to the upper or lower position the unit can be controlled on a local basis by the
76. p and the RS 232 1 connector of the UltiMate Micropump using the Serial Communication Cable item b FIGURE 2 2 e Connect the RS 232 2 connector of the Micropump and the RS 232 1 connector of the UV Detector with the same type of cable UltiMate P N Switchos II Lil 160069 160070 160071 od vRurWerem oP fo PC COM port X SW II Valve Control PC COM port Y Solvent Organizer PC COM port Z ULT SWII Network FIGURE 2 2 Setting up the RS 232 Network Connections If your configuration does not include an UltiMate UV Detector connect the y cable to the RS 232 1 connector of the UltiMate Micropump the RS 232 2 connector of the Switchos loading pump and the COM port of the PC Use a Serial Communication Cable to connect the other RS 232 connectors of both pumps Caution The RS 232 sockets are to be used only with LC Packings software products e g CHROMELEON or third vendor software that support the LC Packings UltiMate System D850R3 User s Manual Switchos Installation and Getting Started 2 3 3 COMMUNICATION Connector Connect the COMMUNICATION port to a free COM port on the computer using the Solvent Organizer Com cable item c FIGURE 2 2 2 3 4 INPUTS Connector CHROMELEON controls the valve positions v
77. plains how to connect a NANO column or other fused silica capillary to the inert PAEK switching valves of the Switchos Il D850R3 User s Manual Switchos II Introduction 1 6 For Additional Information The loading pump that is incorporated in the Switchos Il Advanced Microcolumn Switching Unit is identical to the micropump supplied with the LC Packings UltiMate system If you are using the Switchos I Advanced Microcolumn Switching Unit with the LC Packings UltiMate and or CHROMELEON software please refer to the documentation provided with these products for supplemental information and to the online help of CHROMELEON F1 key In addition to the Switchos II Advanced Microcolumn Switching Unit User s Manual this binder contains a copy of the User s Manual for the LC Packings UltiMate Micropump The UltiMate Micropump User s Manual contains a detailed discussion about the operation and maintenance of the Micropump User s Manual Switchos II D850R3 1 7 Introduction This page intentionally left blank 1 8 D850R3 User s Manual Switchos II gati Installation and Getting Started 2 1 Installation The instructions provided below are provided for installation of the Switchos II Advanced Microcolumn Switching Unit as part of the LC Packings UltiMate Capillary HPLC System as well as for installation of the instrument as a stand alone component in an HPLC system When the S
78. purge valve knob Note The pressure during purging is limited to 0 5 MPa No solvent delivery pump motor is running Air in solvent pump head Purge pump and check degassing Clogged solvent inlet filter Replace solvent inlet filter Leakage in the pump head Check for any leakage Leaking pump head Loose inlet outlet fitting Tighten the fittings Loose check valve s Defective piston seals Tighten the check valves Replace seals Flow fluctuation Flow restriction in the solvent inlet fluid path Air in solvent inlet fluid path Check tubing filters proportioning valves etc Purge pump check degassing Defective valves Clean replace check valves Flow rate too high or too low deviation gt 10 Air in solvent inlet fluid path Purge pump check degassing Pump out of calibration Contact LC Packings User s Manual Switchos II D850R3 6 11 Maintenance and Troubleshooting TABLE 6 2 Troubleshooting the Switching Valves The Switchos does not respond to any signal applied to the INPUTS connector The Switchos is in LOCAL mode e g the LEDs in the push buttons are illuminated Change control mode to REMOTE REMOTE LOCAL switch on the rear panel The switching valves do not respond when pressing the controls push buttons on front rear panel The Solvent Selection valve moves to the next solvent channel and after a s
79. push buttons and switches on front and rear panel Any change that is made will override the present condition regardless of whether it was set via Local or Remote Control When the unit is in LOCAL mode the INPUTS connector is disabled and the built in LEDs in the push buttons are illuminated REMOTE mode when the REMOTE LOCAL switch is set to REMOTE mode the switch is in the middle position the instrument is controlled by an external device In this mode the INPUTS connector is enabled and the built in LEDs and the push buttons are deactivated The switching valves A and B will be positioned according to the current signals of the INPUTS connector eS Note If the instrument is in REMOTE control mode and the INPUTS connector on the rear panel is not connected switching valves A and B are locked in position 1 2 3 4 D850R3 User s Manual Switchos The User Interface 3 5 Basic Operations for the Loading Pump The main screen of the loading pump includes a series of parameters that are used to set the principal operating conditions for delivering the mobile phase such as the flow rate and maximum pressure In automated mode networking these parameters are set via CHROMELEON The following sections provide basic information about the loading pump a detailed discussion on pump control is presented in the UltiMate Micropump User s Manual All communication between the user and the system is provided by the fr
80. r and do not affect the sealing force which is automatically set as the screws close the cap against the valve body c Test the valve by pressurizing the system If the valve does not hold pressure it should be returned for repair ae Note When re installing the valve make certain that the proper tubing is attached to the appropriate fitting A 4 Spare Parts Part Number Description Standard Version Stainless Steel 161472 Replacement rotor for VALCO C2 10 Port 162016 Stator VALCO C2 10 Port Inert Version PAEK 161005 Rotor for Switchos 10 port injection valve VALCO C2 INERT 161006 PAEK Stator for SWITCHOS 10 port valve VALCO C2 INERT PAEK Polyaryletherketone User s Manual Switchos II D850R3 A 3 The Switching Valves This page intentionally left blank A 4 D850R3 User s Manual Switchos II APPENDIX B Network Identification B 1 Identifying the Switchos on the Network The Device Identifier and Serial Number of the Switchos Advanced Microcolumn Switching Unit must be correctly set to ensure proper operation of the unit on the network The Device Identifier and Serial Number is factory set during the manufacturing process It can be checked in the GLP Menu If it becomes necessary to reset the Device Identifier and or the Serial Number the following rules must be used e The Device Identifier for the Switchos must be 91 e The serial number must con
81. ries of activities that can be used to check the operation of the system and to verify that your Switchos Advanced Microcolumn Switching Unit is operating in an acceptable manner This chapter includes e Testing the Solvent Bottle Caps and Degassing Unit Section 5 2 1 e Testing for the Solvent Selection Valve Section 5 2 2 e Testing of the fluid path for the Solvent Selection Valve and solvent lines Section 5 2 3 e Testing of the Switching Valves Section 5 2 4 ae Note Unless otherwise noted pure methanol should be used as the solvent and these tests should be performed at 25 C User s Manual Switchos D850R3 5 1 Testing the Switchos 5 2 Checking System Components 5 2 1 Solvent Bottle Caps and Degassing Unit The He lines the regulating valve and connections can be tested by the following procedure a Close the Shut Off Valves FIGURE 5 1 and apply 3 bar 45 PSI of He pressure caution Caution Do not operate the He lines a higher pressure than 4 bar 60 PSI Vent He Shut Off Valve As FIGURE 5 1 The He Shut Off Valve Opened Position b Open the He Regulating Valve on the front panel The regulating valve should be turned 90 Check to see if there are any leaks i e are He bubbles observed in the solvent bottle c Open the shut off valve on the bottle cap for solvent bottle A by closing the He regulating valve verify that the bubbles of He exit on the side frit of
82. rview This appendix provides instructions how to connect a NANO column or other fused silica capillary to the inert PAEK switching valves of the Switchos II Advanced Microcolumn Switching Unit These directions should be used whenever a NANO column or a fused silica capillary is to be connected to the inert PAEK switching valves of the Switchos Il Caution Do not use a stainless steel nut and or ferrule with inert PAEK injection switching valves The use of stainless steel nuts or ferrules may damage the valve Use only the supplied fittings PEEK and follow the instructions below The PEEK sleeve connection is created using the components shown in FIGURE D 1 e PEEK fingertight nut e PEEK ferrule e PEEK sleeve with an appropriate D e NANO column or any other fused silica capillary e The female fitting port of the inert valve FIGURE D 1 shows the assembly tool required for the assembling purpose only Assembly Tool Sleeve Fingertight Nut DIONEX Ferrule NANO Column FIGURE D 1 Parts of a PEEK Sleeve Connection User s Manual Switchos II D850R3 D 1 Modifications D 2 Fitting Assembly caution Caution Never use the inert PAEK injection switching valve to pre assemble a fitting and never use any tool to tighten the fingertight nut This may lead to damage of the valve To assemble the PEEK sleeve fitting using the supplied stainless steel union a b c Slide the fingert
83. seal of the switching valves Check Stator of the switching valves Solvent Selection Valve for Leakage a a Contact your LC Packings representative eS Note The frequency of the various activities described above is a reasonable starting point As the user gains experience with the system it will be found that some activities can be done less frequently and other need to be done more frequently The frequency is dependent on a number of factors including the nature of the sample and the mobile phase 6 2 D850R3 User s Manual Switchos II Maintenance and Troubleshooting 6 3 Replacing Major Components A variety of components on the Switchos Advanced Microcolumn Switching Unit can be readily changed by the user as required to ensure that the instrument is maintained in optimal condition This section provides information and procedures about how to replace user replaceable parts In most cases re assembly of a component is identical to its disassembly except that the steps are performed in the reverse order If no comment is made it should be assumed that the assembly of a component or installation of a component is identical to disassembly or removal except that the actions are in the reverse order Sections 6 3 3 to 6 3 5 provide basic information about how to remove the pump head and how to replace check valves cartridges or piston seals For more detailed information refer to
84. splay messages of the loading pump is presented in the UltiMate Micropump User s Manual e Sparge the mobile phase and flush the solvent lines Section 2 6 3 e Prepare the fluidics connection according to your needs A typical example is shown in Section 2 6 4 additional examples are presented in Appendix C Caution Do not use a stainless steel nut and or ferrule with the inert PAEK injection switching valves The use of stainless steel nuts or ferrules may damage the valve Use only the supplied fittings PEEK and follow the instructions below If you are using an inert version please refer to Appendix D for important information about how to connect fused silica tubing to the inert switching valves Caution Use the fittings supplied with the instrument Valco or equivalent fittings to ensure that the dead volume of the system is minimized Use of Rheodyne fittings should be avoided Rheodyne fittings are designed differently they will lead to unswept volume and possible system damage 2 6 2 He Connection Connect the Helium line 1 4 O D that is supplied to the Helium inlet on the rear panel of the Switchos FIGURE 2 1 To connect the Helium line of the Switchos to the Helium line of the UltiMate Capillary HPLC System use the T piece P N 161470 that is supplied with the Switchos instrument The Helium pressure should be set to approximately 1 bar Caution Do not operate the He lines at a higher pressure than
85. tain six digits If the serial number of the Switchos loading pump contains only 5 digits add a leading zero e g 72835 should be entered as 072835 In Table B 1 we indicate the identification of various devices including a Switchos on a network TABLE B 1 Identification of a Various Devices on a Network Device Device Serial Number Identifier lower 6 digits 2 Switchos Loading Pump 3 UltiMate Micropump O UltiMate UV Detector 9 left blank O in case the S N has less than 6 digits me Note If the Device Identifier or Serial Number for the Switchos is incorrectly set the unit may not be able to communicate with other devices on the network depending on the control software package User s Manual Switchos II D850R3 B 1 Network Identification This page intentionally left blank B 2 D850R3 User s Manual Switchos II APPENDIX C Cleanup of Proteins C 1 Overview This appendix describes a common application of the Switchos II Advanced Micro Switching Unit and is provided to give the user an understanding of the power and flexibility of the system In this application the two 10 port switching valves are used for on line sample clean up digestion of proteins and peptide separation An ion exchange column and protein digestion column are connected to valve A and a reversed phase trap column and the capillary nano separation column are conn
86. tchos 5 2 4 Switching Valves Basic Test To check the basic function of the Switching Valves and the Valve Controller a Set the Switchos in local mode by the Remote Local switch on the back panel The LEDs of the two push buttons on the front panel should be illuminated b Toggle valve A by the corresponding push button on the front panel The LED corresponding to the current position e g LED 1 2 or LED 10 1 should be illuminated and you should hear the sound of the moving valve for a short period of time c Repeat step b for switching valve B D850R3 User s Manual Switchos one Maintenance and Troubleshooting 6 1 Overview This chapter provides information to assist in optimizing the performance of the Switchos Advanced Microcolumn Switching Unit and maintaining it in your laboratory It includes the following material e Maintenance describes a series of activities that should be performed on a periodic basis to optimize the performance of the system and minimize down time Section 6 2 e Replacing Components provides directions for replacing components e g due to wear Section 6 3 e Troubleshooting discusses a series of activities that should be used to determine the cause of a problem Section 6 4 e Spare Parts Replacement Parts Lists Presents a listing of components that are used to maintain the unit or to change the configuration Section 6 5 User s Manual Switchos II D8
87. ted with the START STOP key item 2 FIGURE 3 5 This key is also used to stop the flow delivery 3 5 4 Purging the Loading Pump To purge the loading pump opening the purge valve and then press the PURGE key on the pump item 1 FIGURE 3 5 To assure proper purging of the solvent lines set the flow rate to 0 5 mL min Select each of the four solvent bottles A B C and D by the SSV button on the rear panel Each of the four solvent channels should be flushed for approximately 2 minutes and the operator should ensure that no air bubbles are observed in the mobile phase Caution Purging the system without opening the purge valve knob may cause damage to your column and or the system 3 6 D850R3 User s Manual Switchos CHAPTER 4 CHROMELEON Control 4 1 Overview This chapter provides information about how to setup the CHROMELEON Software to control the Switchos Il Advanced Microcolumn Switching Unit either by the RS 232 communication port or by the event outputs of the Switchos II loading pump A detailed description of the software features is provided in CHROMELEON online help F1 key and a quick Getting Started reference can be found in the UltiMate User s Manual It is assumed that the user has a basic understanding of the CHROMELEON software and its modules When the Switchos I Advanced Microcolumn Switching Unit is used in conjunction with the LC Packings UltiMate Capillary HPLC System and the
88. the UltiMate Micropump User s Manual 6 3 1 Replacing the Sparging Filter Frit The Sparging Filter Unit is connected via tubing which slides off the unit Over time the filter may become clogged by particulate matter and should be replaced In some cases the filter can be cleaned via an ultrasonic bath depending on the nature of the particulate matter IES Note A clogged filter may lead to poor analytical performance Section 6 4 Troubleshooting To replace the Sparging Filter Frit a Unscrew the Solvent Bottle Caps from the solvent bottles and take the tubing with the frit out b Remove the old frit by pulling the tubing item 1 FIGURE 6 1 off the filter body item 2 FIGURE 6 1 c Insert the He tubing and the solvent tubing into a new Sparging Filter Frit P N 160044 The He tubing should be placed in the hole closer to the He outlet d Gently pull at each line to make sure that it is secure i 1 Solvent Line 758 amp He Outlet Cy Top View gt 2 aie Solvent Inlet FIGURE 6 1 Sparging Filter Frit User s Manual Switchos II D850R3 6 3 Maintenance and Troubleshooting 6 3 2 Replacing the High Pressure In line Filter The high pressure in line filter serves to remove particulate matter from the mobile phase and is considered as a consumable item If excessive pressure is required to deliver the mobile phase indicated on the pump display it is probable that the f
89. the sparging filter frit Check to make sure that it is possible to regulate the He flow by opening the He regulating valves until the maximum is reached 90 d Repeat steps b and c for each solvent bottle 5 2 2 Solvent Selection Valve Basic Test To check the operation of the Solvent Selection Valve and the Valve Controller a Set the Switchos in local mode by the Remote Local switch on the back panel the LEDs of the two push buttons on the front panel should be illuminated b Select the next available solvent channel by the SSV button The corresponding LED should be illuminated and the sound of the moving solvent selection valve should be heard for a few seconds c Repeat step a for each solvent channel 5 2 D850R3 User s Manual Switchos Testing the Switchos 5 2 3 Solvent Selection Valve and Solvent Lines Fluid Path Test To determine if the fluidic path for solvent line is operating properly and if the resistance of the flow path is within the specifications a Fill each solvent bottle with methanol until the fluid level corresponds to the top of the Switchos housing b Open the four He shut off valves on the bottle cap assemblies to open the solvent lines the He supply line has to be shut off for this test c The four solvent lines should be well flushed using the Purge function of the loading pump If is not possible to flush the lines check for clogged or dirty solvent filters sp
90. the analysis of light sensitive samples and are not instrument related When troubleshooting make certain that the application was running in an acceptable fashion before the problems were observed and that nothing has been changed in the application If you are making a change in the protocol change a single parameter at a time and ensure that the effect of each change is well understood D850R3 User s Manual Switchos II Maintenance and Troubleshooting TABLE 6 3 Typical System Parameters of a Pre concentration Setup Parameter Typical values a CAP Configuration NAN Configuration Micropump Flow i Master flow 200 uL min 187 5 uL min Micropump Pyesstye 160 200 bar 160 200 bar Master pressure CRP b Column Flow 300 nL min Column Pressure 60 80 bar 100 130 bar Trap Column Flow 30 uL min 30 uL min Trap Column Pressure 40 70 bar 40 70 bar Response delay Time Notes a b c d 100 mobile phase A start condition of the UltiMate standard system checkout procedure with the cytochrome c test sample No flow sensor for automatic CRP calibration used Used in the micro flow path If a trap column is used in conjunction with the Switchos this is the time after switching the trap column into the micro flow User s Manual Switchos II D850R3 6 13 Maintenance and Troubleshooting 6 5 Spare Parts Lists re Note Please refer to the User s Manual UltiMate Micropu
91. ump Inlet 4 The Inert and the Standard system use the same type of pump head but different connecting tubing 160056 162010 Note 6 5 7 Control Cables Part Number Description 160069 Y Communication cable 160070 Serial Communication Cable 160071 Solvent Organizer Communication Cable 160147 Contact Closure 4x Cable for FAMOS P5 Cable for controlling Switchos by FAMOS P5 Auxiliaries 160171 mojan ana op A Cable for controlling Switchos by UltiMate Event Outputs 160172 lalola I oa 3 A x x daa qq 160184 Set of Connectors WAGO Type x 6 16 D850R3 User s Manual Switchos II CHAPTER 7 Specifications 7 1 Analytical Specifications 7 1 1 10 Port Switching Valves low dispersion 10 port valve 2x 350 bar 5000 psi Wetted Surfaces standard version inert version stator stainless steel PAEK rotor Valcon H Valcon E Diameter local control push button remote control relay open collector or TTL twoLEDspervalve Cd Position Indicator two LEDs per valve 7 1 2 Solvent Selection Valve Wetted Surfaces stator PPS polyphenylene sulfide rotor
92. witchos Advanced Microcolumn Switching Unit is used in conjunction with the UltiMate system and the FAMOS Microautosampler all instruments are controlled by the CHROMELEON software The loading pump will be included in the instrument network and the switching valves will be controlled by a COM port of the PC If no additional COM port is available or if the Switchos is to be installed with different or third vendor software please refer to Sections 2 4 and 2 5 for information about how to control the switching valves by relay outputs e g the Event outputs of the Switchos loading pump or the Auxiliaries of the FAMOS Microautosampler if present Once you have set up the Switchos refer to Section 2 7 for information about routine operation of the system Please refer to the user s manuals of the UltiMate system and the FAMOS Microautosampler and the online help of CHROMELEON for additional information 2 1 1 Location of Switchos in the Laboratory The Switchos Advanced Microcolumn Switching Unit should be installed in a facility with the following environmental conditions e The temperature range should be maintained between 10 and 40 C The system should be installed in an area in which the temperature is fairly constant do not place the system near a window an air conditioning duct or a heating duct The humidity should be maintained between 20 and 80 relative humidity User s Manual Switchos D850R3 2 1 Installat

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