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Bioreactors

1. yal 5 m VAN YE a ya if ae Abs La gt 1 Ss Es va ma T d 7 T Li 1 27 IT lt Le r Bloreactors Complete solutions from micro scale to full scale production 9 100 Ikon BIOTECHNOLOGY a A y s x po a T 4 4 gt 1 ie Y y P 77 j f a j 2 Ee 7 m 24 r a T Y OS i A E 4 VE t p E BEN fr ELA A j Dh lt gt vi E 3 a d A S 1 4 j f 3 T I 4 P gt N p E s kK 7 gt 4 1 L LA te x l it e XE Y n 7 LE oA i a aL j a step ahead 04 06 08 10 12 16 18 20 24 26 28 30 32 36 38 40 42 4 4 46 Micro Flask by Duetz Hambio Alcohol Fermentation Monitor MiniBioreactors Glass autoclavable bioreactors Applikon PhotoBio AppliFlex Single Use Bioreactor BioBundle Cultivation Systems my Control in Control Ez Control i Control BioXpert DO Sensors pH Sensors Biosep Retention system Steam In Place Bioreactors special bioreactor projects AppliCare Co nte nt Applikon Biotechnology Bioreactors OS Applikon Biotechnology a Step Ahead Applikon Biotechnology is a world leader in developing and supplying advanced bioreactor systems from laboratory scale to production scale We can take a customer from the initial screening stage up to full scale production using the same platform This minimizes scale up risks
2. e Metabolic flux studies and high throughput screening for high activity prokaryotic or eukaryotic mutants e Comparative studies e g clinical isolates e Growth medium optimization for cell lines or production strains Specifications Type of Well volume microtiter plate 24 square deep well polypropylene 17x17 mm depth 40 mm 11000 yl 24 round low well polystyrene 16 mm depth 18 mm 96 square deep well polypropylene 8x8 mm depth 40 mm 2400 ul 96 round low well Q 380 Ll polystyrene 6 5 mm depth 11 mm Culture volume 2500 ul 2500 ul 2500 ul 4000 ul 4000 ul 750 ul 750 ul 1000 ul 1000 ul 500 ul 500 ul 750 ul 750 ul 1000 ul 1000 ul 100 ul 100 ul 150 ul 150 ul 200 ul 200 ul Orbital shaking frequency 300 rom 300 rom 220 rpm 300 rom 220 rpm 300 rom 300 rom 300 rom 300 rom 300 rom 300 rom 300 rom 300 rom 300 rom 300 rom 300 rom 300 rpm 300 rpm 300 rpm 220 rpm 300 rpm Shaking amplitude 50 mm 25 mm 50 mm 50 mm 25 mm 50 mm 25 mm 50 mm 25 mm 50 mm 25 mm 50 mm 25 mm 50 mm 25 mm 50 mm 25 mm 50 mm 25 mm 50 mm 25 mm O transfer rate 30 C air 1 bar 51 mmol 0 1 h 39 mmol O5 1 h 35 mmol O5 1 h 24 mmol 0 1 h 24 mmol 0 1 h 40 mmol 0 1 h 25 mmol 0 1 h 30 mmol 0 1 h 19 mmol 0 1 h 38 mmol O5 1 h 12 mmol 0 1 h 24 mmol 0 1 h 7 mmol 0 1
3. Insertion Length Z010011025 insertion length 110 mm 215 mm total length used with Tracfix sensor holder 2010015420 insertion length 154 mm 259 mm total length 2010023525 insertion length 235 mm 340 mm total length Bioreactor 1 5 Ltr 2010032525 insertion length 325 mm 430 mm total length Bioreactor 7 Ltr 2010042525 insertion length 425 mm 530 mm total length Bioreactor 15 Ltr 2010059025 insertion length 590 mm 695 mm total length Bioreactor 20 Ltr Applikon Biotechnology Bioreactors of a 7 Applisens 3 8 Applikon Biotechnology Bioreactors oH Sensors In the biotech and pharmaceutical industry it is vital to have precise information about the OH value of the bioprocess The pH level directly affects viability productivity stability of the cells and it influences analysis of active ingredients The AppliSens pH sensor is specifically designed for long term stable and accurate measurements in bioprocesses The pH sensor has a fixed sleeve diaphragm that reduces the influence of the culture medium on the pH measurement Compared to classic diaphragm types the sleeve diaphragm increases measuring accuracy and increases the lifetime of the sensor which is vital for long lasting biotech cultivation processes Features e Robust design by using tempered glass shaft e Sensor head uses minimal space on bioreactor topplate e Accurate measurement ow sensitivity to fouling due to sleeve diaphragm
4. e Cell culture e Batch e Fed Batch e Perfusion Applikon Biotechnology Bioreactors 1 O an E AppliFlex Rocker Specifications Appliflex Bio One BEEN Total volume TOL ZOE Qe be 120316 L 660 L 1320 L 2700 L mE Working volume SE NOEL 50 L 100 L 250 L 500 L 1000 L 2000 L NEN Dissolved sterilizable pH Polarographic Oxygen Dissolved Oxygen Measuring range 930 0 3 pH O 10096 O 12 pH O 100 Dies LOO NEN O saturation Oo saturation Accuracy 0 1 pH t 0 496 0 1 pH 2 o NEN Measuring temperature range O 40 C O 40 C O 80 C O 60 C Or T1900 E Maximum temperature 45 C 45 C 1856 1850 150 C Sensor life time 2 weeks O 6 months 5 1 year gt 1 year gt 1 year mE 1 measurement per minute 3 5 weeks Q 1 measurement per 2 minutes 70 Applikon Biotechnology Bioreactors SloBunale complete cultivation systems A BloBunale is a complete bioreactor system equipped with all necessary components and is ready to use out of the box No detail is overlooked the system is complete with silicone tubing sample bottles and a starter kit including spare parts The BioBunale IS easy to set up requires no special skills or tools easy to learn and easy to operate BioXpert Lite Software for data acquisition is included Select one or more of the optional add on packs to customize your BioBundle The BioBuncle provides a unique Ethernet computer interface allows ea
5. All accessories such as pumps and gas flow control valves are conveniently integrated in a compact console The compact design reduces the need for expensive lab space software for data acquisition and supervisory control The BioBundle control system includes an Ethernet connection port to connect to a PC with software for data acquisition or SCADA such as the Applikon BioXpert packages BioXpert Lite data acquisition is included in this BioBundle Multiple bioreactors can be connected to one PC for data acquisition Optional BioXpert packages are BioXpert 2 and BioXpert W7 SCADA of multiple bioreactors including cGMP production applications Cost Saving The BioBundle is a pre packed system The assembling of the BioBundles in series provides a cost saving which is for the benefit of the customer oummarising Over all some of the benefits Applikon bioreactors provide include e dependable and reliable operation greater productivity and yield e modular design less cost in the future being able to use same equipment for different applications e less downtime due to maintenance greater economy in operation higher yield to cost ratio e simple operation less operator training required the product Specifications Control system Total Volume Working Volume Agitator Aeration Exhaust gas Sampling pH Temperature Dissolved Oxygen Foam Level Liquid additions Start up kit BioXpert L
6. Australia Enztech Pty Ltd Phone 61 2 9360 4971 www enztech com au Middle East 4 Africa UAE Alliance Global FZ LLC Tel 497143752744 www agbl net Isra l Medent LTD Phone 972 86237984 www medent co il QIOOIKOCT BIOTECHNOLOGY G Applikon Biotechnology BV a step ahead Applikon Product Overview V3X0120520 Subject to modifications Printed by Applikon Biotechnology bv The Netherlands 2012 ISO 9001 REGISTERED FIRM Reg No 24 P O Box 101 3100 AC Schiedam The Netherlands Nieuwpoortweg 12 3125 AP Schiedam The Netherlands T 31 0 10 208 35 55 F 31 0 10 208 35 05 E info applikon biotechnology com DNV CERTIFICATION B V THE NETHERLANDS www applikon biotechnology com
7. e Stable signal over longer time e FDA approved materials e Withstands repeated SIP and CIP cycles e Standardized PG 13 5 connection guarantees interchangeability with other bioreactor brands Applications e Microbial cultures e Cell cultures e Batch e Fed Batch e Continuous cultures e Perfusion cultures Applikon Biotechnology Bioreactors 39 Specifications E Parameter As Delivered pH range Q 12 pH mE Temperature range 0 135 C mnm Electrode zero point E7 15 mV E Electrode slope S4 7 9896 Isothermal Intersection pH Eiso 6 5 8 PH Range 4 9 Membrane resistance O 25 C MQ 1200 Membrane resistance O 37 C MQ 500 Calibration drift in Buffer 9 mV Min 2 Stirring Error Buffer 9 O 25 C pH 0 05 Drift 72 hours after Autoclaving pH 0 13 Zero point drift per week in PBS pH lt D OS Response Time pH 9 to 4 25 C in Seconds lt 90 Response Time 90 pH 9 to 4 25 C in Seconds 45 lt lt lt Response Time pH 9 to 4 37 C in Seconds lt 45 B Response Time 90 pH 9 to 4 37 C in Seconds 45 ESEL Sensor diameter 12 mm Sensor length insertion length total length Article nr Z001012051 120 170 mm _ Z001023551 235 185 mm _ Z001032551 325 375 mm __ Z001042551 425 475 mm Z001059051 590 640 mm p Sensor electrical connection K9 AC Applikon Biotechnology Bioreactors Blo
8. and guarantees the shortest time to market for our customers new product development New technologies Applikon is known for bringing new technologies to the market These new technologies offer advantages in process efficiency for research and development as well as pilot plant and production scale processes Your advantage Working with the latest technology offering a competitive advantage in efficiency and reliability Reliable As a privately owned company our focus is on building a healthy company by supplying the best product offering for our customers now and in the long term future This is the basis of many long term relations with our clients all over the world Your advantage Applikon can always support you through the well trained sales consultants and technicians of our technical support department High lech Applikon Biotechnology is unique in the mini and micro bioreactor range No other company can offer this complete solution on very small scale We develop small scale systems to generate results that can be scaled to production scale There are thousands of Applikon bioreactors used in the world from small scale R amp D up to full scale cGMP production Your advantage The best solution from micro scale to full scale production resulting in the shortest time to market for your new products Worldwide activities oince we started in 1974 we have shown a healthy growth resulting in a global market leader role R amp D
9. design and engineering are in house in our headquarters in Schiedam The Netherlands We have our own sales and service organizations in the USA the UK and China We have well trained local distributors for sales and service in over 20 countries Your advantage No matter where you are you can always rely on Applikon now and in the future Qur mission providing reliable solutions for the bioprocess market that will enable an improved quality of life G QIOOIKO CT BIOTECHNOLOGY a step ahead A Applikon Biotechnology Bioreactors MICrO Flask by Duetz Cultivation In microtiter plates The Micro Flask system facilitates reproducible and reliable culturing on microtiter plates The system consists of sandwich covers cover clamps and cryo replicator The Micro Flask enables a single person to grow and test thousands of strains EN simultaneously with a minimum of repetitive hanaling EN Features e Conversion of 24 and 96 microtiter plates both deep and low well plates into individual micro reactors e ow and uniform evaporation rates for every well e Sterile barrier for individual wells prevents cross contamination e Oxygen transfer rates similar to shake flasks in standard orbital shakers e Simultaneous and reproducible sampling of 96 frozen glycerol stocks Applications e High throughput screening and distribution of mutant and construct libraries e g in E coli or yeast CULTURE
10. flow chart no no yes a Combination of current and historic data in graphs yes yes yes Combination of different active batches in graphs yes yes Measured data table display yes yes yes a Scatter plots yes yes yes Storage of predefined graph settings yes yes yes a Supervisory control Programming of time based actions no yes yes mum Programming of event based actions no yes yes ENSE Profiling of setpoints no yes yes Manual setting of local control set points no yes yes Recipe definition no yes yes lc Other mn Notebook for process and EE equipment remarks yes yes yes Event viewer for system comments yes yes yes Audit trail no no yes Batch reports supported no no yes Remote access no no yes 36 Applikon Biotechnology Bioreactors DO Sensors Measurements of dissolved oxygen DO in biotechnological processes IS a basis for process optimization and allows to maximize the product yield A microorganism or cell responds to the oxygen concentration in regulating its overall metabolism Therefore the knowledge of the DO concentration and the proper control during the process are of great importance The AppliSens Low Drift DO2 sensor is specifically designed for long term stable and accurate measurements in bioprocesses The DO2 sensor has a titanium membrane module to minimize the measurement drift The autoclavable polarization module allows polarizing of yo
11. standard Solution e Reliable control platform Allen Bradley and Siemens hard and software e Supplier independent solution based on industry standard hardware e ocal Control and local display e ntegrates with any factory automation system e Cost effective e Fully documented supply eases validation e Can be used in a 21CFR part 11 compliant system Applications e Laboratory scale bioreactors e e Pilot plant scale bioreactors e Production scale bioreactors e Microbial cultures BEEN e Cell cultures COGO M da PN T La e oR Ae aL B e E E J 1 Specifications Applikon Biotechnology Bioreactors 31 pH Temp input pH amplifier Range O pH 14 pH Accuracy 0 01 pH Temperature amplifier Type Pt 100 3 wire type Range OC 150 C Accuracy 0 1 C DO Level input DO amplifier Type Polarographic Range O 96 500 air Accuracy 0 1 96 Level amplifier Type On Off signal Sensitivity Software selectable High Conductivity 2 26 uS equals Contact Low Conductivity 200 uS equals Contact 2Channel analog inputs Hange 4 20 mA Accuracy 0 196 Full Scale value 2Channel analog outputs Range 4 20 mA Accuracy 0 196 Full Scale value 2Channel analog inputs for RTDs Sensor Type Pt 100 Range 200 C 800 C Accuracy 0 2 Full Scale value Resolution 0 1 C Loddo Gus e Ee EE S Moo M 1 Lodo A0070NN931010 2 Appli
12. your present system Applikon will provide a comprehensive quote on time and cost analysis for the upgrade Other services offered e Mass Flow Controller Verification MFC Calibration on a yearly basis is very expensive amp time consuming to a customer AppliCare provides a service called MFC Verification which will indicate if the Flow Controller is within specifications and therefore no calibration would be necessary at the time of your Preventive Maintenance This is done at a fraction of the cost of Standard calibration in the security amp safety of your own lab e Technical phone support Many times our service engineers can diagnose problems over the phone because they are trained to ask the insightful questions e Spare parts Stocking spare parts can be an expensive undertaking especially when Applikon equipment is built with quality 25 years of quality Applikon keeps the recommended spare parts of our standard systems in stock for quick deliveries Applikon Biotechnology Bioreactors f Corporate Office Netherlands Applikon Biotechnology B V Phone 31 10 2083555 www applikon biotechnology com America USA Canada Applikon Biotechnology Inc Main US Offices Phone 650 578 1396 www applikonbio com Applikon Biotechnology Inc East Coast Office Phone 973 361 5387 Mexico Applikon Biotechnology Mexico Phone 52 55 5868 7814 Brasil Alem Mar Phone 11 3229 8344 www allemmar com br Euro
13. 129 15 liter Bio Bench 15 10 4 2 1 1 4 20 liter Bio Bench 20 15 4 3 0 22 30 liter Bio Bench 30 ZAD f 2 0 1 6 30 liter Pilot Cell 30 20 T us 1 0 60 liter Pilot Cell 60 40 10 145 1 0 130 liter Pilot Cell 130 100 28 129 1 0 20 liter Pilot Microbial 20 15 4 3 0 2 2 40 liter Pilot Microbial 40 30 Faa 3 0 2 2 70 liter Pilot Microbial 70 50 10 3 0 Ze 140 liter Pilot Microbial 140 100 20 3 0 2 2 Custom build bioreactor systems are available up to 2000 liter total volume Drive system Direct drive magnetically coupled optional mechanical seal bottom or top mounted agitator for microbial cultures and top mounted for cell cultere systems Maximum agitator tipspeed m s 5 m s for microbial cultures and 1 m s for cell cultures Impellers Gas sparger Gas overlay Rushton and marine with outside diameters 0 33 0 5 vessel diameter Porous sparger or Ring type sparger Optional gas overlay line Exhaust gas Water cooled exhaust gas condenser with internal spiral and or jacketed Sampling Optional resterilizable sample system in DN25 port in lower side wall Draining Resterilizable bottom mounted bellows drain Additions Sterilizable additions push valves and resterilizable addition ports pH Measurement 12 mm classic pH sensor in DN25 port in lower side wall Control via acid pump or CO gas rotameter or MFC in combination with alkali pump DO Measurement 12 mm polarographic DO sensor in DN25 port in lower side wa
14. Android phone e Enhanced gas addition strategies via up to 4 Mass Flow Controllers e Expandable control system with optional extra inputs and outputs e USB connection for optional Biomass or Fluorophor pH and DO sensors and balances e Ethernet communication to SCADA Applications e Replacement of ADI 1010 Bio Controller and ADI 1030 Bio Controller e Autoclavable bioreactors up to 20 liter volume e Single Use Bioreactors e Microbial and Cell cultivation e Batch Fed Batch Perfusion and Continuous cultivation Applikon Biotechnology Bioreactors PF Specifications o Inputs e pH temperature dissolved oxygen agitation foam and level e Optional biomass and fluorophor pH and fluorophor dissolved oxygen e Optional inputs 4 x analog in 0 10 V Control type PID control with selectable autotuning adaptive control Actuators e 8 x digital outputs for heating cooling fixed speed pump solenoid valves etc e up to 4 Mass Flow Controllers or 4 solenoid valves for gas flow control e Spare l O 4 x analog out 0 4 20 mA Additional features Emergency stop connection central alarm output Power Pmax 480 VA 230 115 Vac _ __ Dimensions dxwxh 322 x 312 x 210 mm _ Weight kg 7 2 kg gt MO TOR 2 8 Applikon Biotechnology Bioreactors ez Control total control at your fingertips The ez Control accurately controls pH Temperature Dissolved Oxygen Foam Level and Agitation in bioreactors and fermentors T
15. IP67 for Light bank 16x2 Backlit LCD Feather touch Keys Full duplex RS232 or RS485 interface optional O C up to 60 C 115 230 V AC 50 60 Hz maximum 150 W The PhotoBio range of bioreactors consist of the following products PhotoBioSTR2 PhotoBioSTR3 PhotoBioSTR5 PhotoBioSTR7 PhotoBioSTR15 PhotoBioSTR20 PhotoBioSU10 PhotoBioSU20 PhotoBioSU50 2 liter stirred tank bioreactors single wall non jacketed 3 liter stirred tank bioreactors single wall non jacketed 5 liter stirred tank bioreactors single wall non jacketed 7 liter stirred tank bioreactors Single wall non jacketed 15 liter stirred tank bioreactors single wall non jacketed 20 liter stirred tank bioreactors single wall non jacketed 10 liter single use Appliflex rocking bioreactors 20 liter single use Appliflex rocking bioreactors 50 liter single use Appliflex rocking bioreactors 40 Watt panel 40 Watt panel 60 Watt panel 60 Watt panel 120 Watt panel 120 Watt panel 40 Watt panel 60 Watt panel 120 Watt panel 1 e Applikon Biotechnology Bioreactors single Use Bioreactors Applikon offers two types of single use bioreactors AppliFlex bioreactors from 10 to 50 liter based on mixing by rocking motion and Bio One stirred tank bioreactors in the range of 50 to 2000 liter total volume Both systems are unique in their accurate measurement and control of important process parameters Both systems can be equipped with re usa
16. ality control of traditional feedstock such as molasses e Conversion of wort into beer e Conversion of grape juice into wine e Alcohol tolerance and toxicity studies e ndustrial and academic research on yeast and potable alcohol bio ethanol bio butanol production Specifications Total volume liter Working volume liter Minimum working volume liter Drive system Maximum stirrerspeed rpm Impellers Exhaust gas Temperature Dimensions DxWxH Empty Weight kg 6 reactors of 250 ml or 500 ml 200 ml or 400 ml 50 ml or 100 ml Magnetically coupled drive Standard range is 50 500 Marine with XX mm outside diameter Exhaust gas connection Measurement Pt 100 sensor in central stirrer bar Control heating via central stirrer bar 30 X 85 XOT cm 75 Kg Applikon Biotechnology Bioreactors 09 TM e 1 Applikon Biotechnology Bioreactors Mini Bloreactors real small real bioreactors The MiniBio range of bioreactors 250 ml 500 ml and 1000 ml total volume is a true scale down of the laboratory scale bioreactor he MiniBio systems have the same flexibility as the laboratory scale bioreactors This means that the MiniBio systems can be customized to fit the demandas of any process he small volume reduces media costs and maximises bench space which is normally at a premium Features Applications e Generate more data in less time e Screening studies e Easy setup and operation e Me
17. ble sensors and or fluorophor pH and Dissolved Oxygen sensors The Appliflex bioreactor range consists of a 10 liter 20 liter and 50 liter bioreactor bag The control system pH Dissolved Oxygen temperature mixing is the standard Applikon ez Control Bio One uses the Hy Clone 50 100 250 500 1000 and 2000 liter bioreactors in combination with our ez Control or i Control process controllers Systems are supplied tumkey and fully documented All systems go through standard IQ OQ and FAT procedures before they are delivered and installed at the customers site The qualified Applikon technical support team can take care of the installation and will be your first point of contact for any questions Our delivery includes the bioreactor the controller sensors actuators temperature control system supervisory software and validation documentation Our well trained sales consultants are able to advise you on the complete configuration of your single use bioreactor system Features e Simple operation e OPC compliant controller e Accurate process control e Classic and single use fluorophor sensors for measurement of pH Dissolved Oxygen e Accurate control of pH DO temperature mixing e Data logging 4 SCADA 21CFR part 11 available e Turn key delivery e Q OQ FAT and SAT for the complete system e Fully documented delivery eases validation e One point of contact for the complete system e Qualified support and service Applications
18. c processing and cGMP and GAMP validation reguirements complements the laboratory scale bioreactor systems Scale up from laboratory scale to pilot plant and small scale production is simplified by the consistent bioreactor design and the scalable control solutions All systems are designed to be cleaned in place Applikon offers CIP systems ranging from fully manual control to fully automated Standardized bioreactor systems are available up to 140 liter total volume and custom build units can be supplied up to 2000 liter total volume Features e Easy to clean mirror polished external finish e Electropolished finish of all parts in contact with the culture Ra 0 4 um to allow efficient clean in place e Modular design allows easy adaptation to changing process demands e Magnetically coupled agitator for peace of mind e cGMP compliant design simplifies validation e Compact design reduces floor space needed e Open frame construction gives easy access for maintenance and operation Applications e Scale up studies e Medium optimization e Process optimization e Small scale production e Microbial and Cell culture e Batch Fed Batch Perfusion and Continuous cultivation rotrecunortoar Applikon Biotechnology Bioreactors ARS Specifications Total volume Working Minimum Aspect Aspect liter volume liter working ratio total ratio working volume liter volume volume 7 liter Bio Bench T 5 2 2 2
19. dia optimization e Cultivate using less medium e Process optimization e Cultivate using less bench space e Microbial and Cell culture e Generate scalable results Batch Fed Batch Perfusion and Continuous cultivation L Li f e E N a TEEN Specifications Total volume ml Working volume ml Minimum working volume ml Aspect ratio total volume Aspect ratio working volume Dimensions dxh Dimensions for autoclaving dxh Weight kg Drive system Maximum stirrer speed rpm Maximum impeller tip speed m s Impellers Gas sparger Gas overlay Exhaust gas Sampling Draining Additions pH DO Temperature Foam Level Optional inlets Optional sensors Applikon Biotechnology Bioreactors 1 1 MiniBio 250 MiniBio 500 MiniBio 1000 290 550 1000 200 400 800 50 100 200 2 3 2 1 2 3 1 6 1 5 1 9 180 x 400 mm 195 x 400 mm 200 x 550 mm 180 x 250 mm 195 x 250 mm 200 x 400 mm Direct drive lipsealed 50 2000 2 3 Direct drive lipsealed 50 1750 2 0 Direct drive lipsealed 50 1500 3 05 Choice of Rushton and marine Porous sparger or L type sparger Yes Electrically cooled exhaust gas condenser evaporation 396 per day at 37 C 2vvm Fixed sample pipe with optional sampling system Height adjustable drain pipe 4 fixed inlet ports and optional micro liquid injectors Measurement 6 mm classic pH sensor Control via acid pump variable speed pump or CO gas in combina
20. ed at user defined intervals Off line measurements can be added to the process data to be used for calculations or advanced process control BioXpert offers different ways of presenting your measured data trend graph table and bar graph presentation The on line calculations of BioXpert can be used to calculate derived variables The statement editor and interpreter allow time and or event based execution of advanced control strategies Another option for supervisory control is the use of timed profiles Remote Access for BioXpert XP makes process data accessible for monitoring throughout a network using web browser technology Applications D e BioXpert software can be used for Microbial and Cell cultures in Batch Fed Batch Perfusion and Continuous cultivation 3 A Applikon Biotechnology Bioreactors Specifications Specifications BioXpert Lite BioXpert 2 BioXpert W7 General Operating system Windows NT Windows NT Windows XP service pack 3 Communication drivers include 2000 XP Vista Windows 7 32 bit ADI 1010 ADI 1020 ADI 1030 2000 XP Vista Windows 7 32 bit ADI 1010 ADI 1020 ADI 1030 ADI 1060 Vista Windows 7 32 bit ADI 1010 ADI 1030 ADI 1060 my Control my Control my Control via OPC ez Control ez Control ez Control via OPC in Control in Control in Control via OPC AppliFlex Rocker AppliFlex Rocker i Control via OPC AppliFlex Rocker Balances Balance
21. h 18 mmol Oo 1 h 3 mmol 057 1 h 39 mmol 05 1 h 20 mmol 0 1 h 32 mmol 0 1 h 16 mmol 0 1 h 12 mmol 0 1 h 12 mmol 0 1 h ols and Mio ra nto itis buds E d t CO Headspace refreshment rate 2 5 ml min 1 VVM 2 5 ml min 0 6 VVM 1 1 ml min 1 4 VVM 1 1 ml min 1 1 VVM 1 ml min 2 VVM 1 ml min 1 3 WM 1 ml min 1 VVM 250 ul min 2 5 WM 250 ul min 1 7 VVM 250 ul min 1 3 VVM Applikon Biotechnology Bioreactors 5 Mixing pattern Evaporati poration at 300 rpm rate per well ampl ampl at 30 C 25 mm 50 mm 50 humidity 50 ul H50 per day 75 humidity 25 yl H O per day 2500 ul 1000 yl 50 humidity 30 ul H5O per day 75 humidity 15 ul H50 per day 1000 ul 750 pl 150 ul 5096 humidity 22 ul HO per day 75 humidity 11 pl H5O per day 750 ul 50 humidity 6 ul H50 per day 75 humidity 3 ul H50 per day 150 ul 06 Applikon Biotechnology Bioreactors Hambio tne high intensity mixer The RAMbio bench top biological mixer utilizes ResonantAcoustic technology that applies low frequency high intensity acoustic energy to agitate microbial cultures mE The RAMbio is a highly efficient mixer incubator that dramatically enhances oxygenation and bulk mixing for bacterial and fungal cultures Combined with the patented Oxy EN Pump stopper oxygen transfer rates up to 6 f
22. he color touch screen interface guides the user through the intuitive operation he adaptive control features allow the user to focus on the process while the controller keeps tight control on the important process parameters What makes this controller unique are its ease of use its small footprint its flexibility and its accurate control The optional ADDA board offers an additional 8 analog inputs 4 analog outputs 8 digital inputs and 8 digital outputs Features e Simple operation e Adaptive PID control e OPC compliance e User definable controlloop configuration e Store copy and reload process configurations e ocal data storage and display up to 72 hours e Synoptic trending and bar graph displays e Multiple user levels Applications e Autoclavable bioreactors e Steam in Place bioreactors e Single Use Bioreactors e Microbial and Cell cultivation e Batch Fed Batch Perfusion and Continuous cultivation Q oco TT ER Versa Specifications Measurement range Measurement accuracy pH O 14 pH 0 01 pH Temperature O 150 C 0 1 C Dissolved Oxygen O 100 saturation 0 1 Foam Level Agitation O 1250 rpm 1 rpm O 2000 rpm 1 rpm Analog in 8x Analog out 4 x Digital in 8x Digital out 8x Fluorophor pH optional 0 5 8 5 pH 0 1 pH Fluorophor Dissolved Oxygen optional O 100 saturation 0 1 Biomass optional Display 10 4 Inch color display with resistive touchscreen NEN Communicatio
23. ications Over the past years we have generated a large library of customized solutions for a variety of specific demands This guarantees that we can supply our customers with a proven solution for virtually any process demand Since we are standardized on supplying products for the pharmaceutical industry all our products and processes are fully documented Our ISO 9001 certificate underlines the high quality level of our work and accompanying documentation Ab Applikon Biotechnology Bioreactors AppliCare total process control What separates our service engineers from other organizations is that our people are _ BioProcessing Specialists This means that not only do we know how to maintain and repair equipment we can also troubleshoot because we understand process control We specialize in bioreactors controllers software motors probes and all phases of your EE specific system s operation Applikon s AppliCare will fix your problems guaranteed E We help to eliminate the lost hours incurred to your production schedule and the high costs of downtime Installation and training A correctly installed system with comprehensive on site training minimizes set up time and ensures optimum operation within the shortest timeframe The installation service incorporates e Pre plan design amp Supply Service Requirement over view before arrival of Applikon Equipment into your lab e Connection of all tubing h
24. ite software MiniBioBundle Cell Culture my Control 250ml 500 ml 1000ml 200ml 400ml 800ml Lipsealed with marine impeller Air supply via sparger Optional gas outlet Sample pipe included Sample system optional Measurement via pH sensor control via liquid alkali pump and COs gas supply Measurement via Pt 100 Heating only via heating blanket Measurement via DO sensor control via Air and Oo gas supply Option Option 4 fixed in topplate and 1 septum port One liquid storage system included Included Included MiniBioBundle Microbial my Control 250ml 500 ml 1000ml 200ml 400ml 800ml Lipsealed with Rushton impellers Air supply via sparger Gas outlet condenser Sample pipe included Sample system optional Measurement via pH sensor control via liquid alkali or acid addition pump Measurement via Pt 100 Heating and cooling via Peltier system Measurement via DO sensor control via Air supply and agitation speed Measurement via Foam sensor control via anti foam addition pump Option 4 fixed in topplate and 1 septum port Two liquid storage systems included Included Included BioBundle Cell Culture ez Control 1L 2L 3L alt 7L 15L 20L DOE TL ATL 3 2L 5 4L 12L 16L Lipsealed with marine impeller Air and Oxygen supply via sparger Gas outlet condenser Sample pipe included Sample system optional Measurement via pH sensor con
25. kon Biotechnology Bioreactors A BioXpert Supervisory control and data aquisition BioXpert is a software tool to control bio processes and to store the process data _ The software is designed specifically for biotech researchers and operators in the blo environment The logical structure of the program and the user friendly interface allows the program to be used by anyone That is why over 3 000 copies of our BioXpert software NEN are in use worldwide The software is easy to operate and has extensive functionality for q monitoring collecting and storing data and controling processes Various BioXpert versions have been developed based on international standards for use in either research and pilot plant or production environments BioXpert Lite is designed for data collection data storage and data presentation to set up and to modify processes The pilot plant and production version BioXpert W7 focuses on process repeatability and data security E th Applikon E Taa on Biotecl plikon Bi The research version BioXpert 2 offers maximum flexibility l l l l l l l l _ Features BioXpert seamlessly integrates with Applikon and other makes of controllers and peripheral equipment to enable you to get the maximum out of your cultivation system Pre defined recipes exist for microbial culture mammalian cell culture and plant cell culture All process data and controller data can be stor
26. l processing is enhanced by allowing up to 32 my Controllers in one human interface e Extended liquid additions options tuned to small scale cultivation via up to 6 digital variable speed pumps or micro addition valves for extremely accurate nanoliter volumes e Enhanced gas addition strategies via 3 mass flow controllers e No water connections needed due to electrical cooling and heating system for bioreactor and condenser e Expandable control system with optional extra inputs and outputs e USB connection for optional balances Biomass or Fluorophor pH and DO sensors Applications e deal for screening media optimisation modelling toxicity studies etc e Mini bioreactors e Autoclavable bioreactors up to 3 liter volume e Single Use Bioreactors up to 3 liter volume e Microbial and Cell cultivation e Batch Fed Batch Perfusion and Continuous cultivation Applikon Biotechnology Bioreactors 2 5 Specifications Inputs pH temperature dissolved oxygen agitation foam and level Optional biomass and fluorophor pH and fluorophor dissolved oxygen Optional inputs 4 x analog in O 10 V Control type PID control with selectable autotuning adaptive control Actuators up to 6 digital variable speed pumps or micro addition valves for addition of extremely accurate nanoliter volumes up to 3 Mass Flow Controllers or 4 solenoid valves for gas flow control Flectrical bioreactor heating via Peltier element or Heating Blanket Flectrical bioreac
27. ll Control via a combination of N2 Air Oo Rotameter or MFC and agitation or nutrient addition pump Temperature Measurement Pt 100 sensor in in DN25 port in lower side wall Cultivation control cooling and heating jacket via bioreactor wall Foam Measurement Height adjustable conductivity based foam sensor Control Anti foam addition pump Level Measurement Height adjustable conductivity based level sensor or I oadcells in bioreactor frame Control pump for liquid addition or removal Oppllko y p t opecial Projects Applikon Biotechnology Bioreactors 4 5 customizea solutions some customer demands cannot be fulfilled with our standard product offerings In these cases we build custom solutions for our customers These projects can range from very special mini bioreactors to a complete line of CGMP production systems ranging up to several thousand liters volume systems Key to the success of these projects is our experience of over 35 years building customized bioreactor solutions in combination with clear communication between our project team and the customer We have developed special web based tools for communication and open information exchange within the project team This ensures that everybody is working with the same up to date information all the time The result of this approach is a customized bioreactor system that is delivered on time and completely according to the customer s specif
28. ly Option Option One inoculum and alkali inlet One liquid storage system included Included Included Pg 4 Applikon Biotechnology Bioreactors my Control Color your lab my Control is the most advanced bioreactor controller for small scale bioreactors starting at 50ml working volume The system can control bioreactors up to a total volume of 3L This versatile controller can be used for both cell culture and microbial cultures The advanced software makes it possible to switch from microbial to cell culture configuration in seconds With its footprint of only 19 by 39 cm W x D it uses the minimal amount of bench space allowing to set up as many as 5 on 1 m width of bench space The built in a web server allows the my Control to be operated by any computer with a web browser Internet Explorer Mozilla firefox Apple Safari or Google chrome Wireless devices like iPad iPhone or android tablets or phones can also be used to operate my Control The selectable colored band on the unit allows the system to be personalized and to fit your laboratory Features e Control of all bioprocess parameters including pH temperature dissolved oxygen agitation foam and level e Selectable autotuning adaptive PID control for accurate control when process conditions change during the culture e Easy operation through web browsers Windows PC Apple PC Linux PC iPhone iPad Android tablet Android phone e Paralle
29. n interfaces e x Ethernet port computer communication e 2 x USB slave e x USB master Control type PID control with selectable auto tuning adaptive control Actuators e up to 3 fixed speed pumps e up to 4 Mass Flow Controllers e heating and cooling via thermocirculator heating blanket cooling water valve e water cooled condenser connection Additional features e Emergenoy stop connection central alarm output e Color touch screen operation Process connections e 2 x Gas outlet water for condenser in and out e water for temperature control in and out Power 230 Vac 50 Hb 115 Vac 60 Hb Dimensions dxwxh 474 x 408 x 711 mm Weight kg 30 kg 30 Applikon Biotechnology Bioreactors Control the scalable contro system i Control is an easy to operate bioreactor control system and utility console EN This scalable control solution is used to control processes in bioreactor systems from laboratory scale to pilot plant and production scale saving time and money during scale up he i Control is available in a Single a Dual bioreactor set up as well as in a Quad 4 bioreactors configuration This standard control solution can be supplied with uM Allen Bradley or Siemens PLC s Basic functionality includes advanced process control for numerous parameters fully automatic sterilization and Clean In Place routines The off the shelf system is supplied pre configured and ready to use mu Features e Off the shelf
30. n low initial investment and low running costs The stirred tank reactor STR is the most widely used bioreactor type Glass autoclavable bioreactors and fermentors are available in 1 2 3 5 7 15 and 20 liter total volume Features e Wide range of volumes with interchangeable modules e Wide range of modules to tailor the systems to the research demands e No welded parts in the bioreactor topplate e Simple set up and easy to handle e Optional high torque magnetically coupled agitator e All metal parts are constructed of stainless steel 316L e External mirror polished finish e Electropolished finish of product contact stainless steel parts ease cleanability e Glass dished bottom vessels are made of borosilicate glass to guarantee resistance to thermal shock excellent corrosion resistance smooth non porous surface for easy cleaning optimal transparency for visual inspection of the culture e Glass bioreactor vessels can be used up to 0 5 barg 7 5 psig of overpressure Applications e Microbial cultures e Cell cultures e Batch e Fed Batch ee i A UU P 1 a gt d i b ES LIE e Perfusion and e Continuous cultivation 1 A Applikon Biotechnology Bioreactors Specifications 1 liter 2 liter single wall 2 liter jacketed 3 liter single wall 3 liter jacketed 5 liter single wall 5 liter jacketed 7 liter single wall 7 liter jacketed 15 liter
31. ndenser Fixed height or height adjustable sample pipe with optional sampling system Sample pipe internal diameters choices are 1 7 mm 4 mm 6 mm or 10 mm Drain pipe Triple or single inlet ports and optional micro liquid injectors Measurement 12 mm classic pH sensor Control via acid pump or CO gas rotameter or MFC in combination with alkali pump Measurement 12 mm classic polarographic DO sensor Control via a combination of No Air Oo Rotameter or MFC and agitation or nutrient addition pump Measurement Pt 100 sensor in thermowell in topplate Control cooling and or heating jacket via bioreactor wall or via internal heat exchanger Measurement Height adjustable conductivity based foam sensor Control Anti foam addition pump Measurement Height adjustable conductivity based level sensor Control pump for liquid addition or removal Septum chemostat tube liquid entry system 1 6 Applikon Biotechnology Bioreactors Pnoto Bloreactors the next best thing to sunlight The Applikon PhotoBio photobioreactor range is based on the standard Applikon mM re usable and single use bioreactors Special light panels are added to these systems to a allow the growth of photosynthesizing organisms plants algae bacteria The well proven design of the bioreactors in combination with the state of the art LED lignt panels guarantee the best performance of the PhotoBio for any application The light panels can be retrofit
32. old higher than orbital shaken cultures can be achieved m Features e Increased oxygen transfer for aerobic cultures e Rapid turnover of any desired gas e Take advantage of richer media formulations e 2 6 fold increase in plateau biomass levels e Up to 10 fold increase in product expression e Up to 6 fold increase in Oxygen Transfer Rate e Up to 5 times shorter cultivation time e Humidity control ix 50 mf 4 2007 250 mj 200 Y 4 SC NEX gt A gt 1 5U x 100 fa 5 z _ A o Shake flask under the influence of ResonantAcoustics opecifications Capacity Dimensions Temperature control Weight Humidity control Power Mixing power 18 x 250 mi flasks or 14 x 500 ml flasks or 8 x 1000 ml flasks 102 x 57 x 70 cm W D H 40 x 27 x 23 Ambient 5 C to 60 C 200 kg 440 lbs Ambient to 90 RH 110 230 VAC 50 60 Hz 3 20g acceleration 196 m sec 09 Applikon Biotechnology Bioreactors Alcohol Fermentation Monitor simple parallel cuttivation The Alcohol Fermentation Monitor or AFM is a robust and user friendly laboratory parallel fermentation system that allows for accurate comparisons of carbon conversion rates and yields for six simultaneous anaerobic fermentations Monitoring the amount of CO2 g that evolves from a fermentation broth under well controlled conditions is a reliable comparison method that has pr
33. oses or piping from the Applikon Control Skid to the Supply Services or the Utility Panels e Installation of all cabling from SCADA software to the controllers S e Site acceptance tests Preventive maintenance Downtime and emergency call outs can be costly and always happen gt unexpectedly during an important run Preventive maintenance extends the lifetime of the major components of your system preventing premature wear and failure Benefits of Applikon s service agreements _ gt e Reduced charges for emergency repairs e Free access to firmware upgrades e Reduced labour charges for any upgrade work or refresher training on site Extended warranty This plan is a two year extension of the original equipment warranty and includes parts and labor as needed Instrument calibration service e Validation e Calibration certification Offering a new breakthrough Retrofitting Make your old vessel new again With the introduction of the i Control ez Control and in Control it is now possible to upgrade both Autoclavable and Steam in Place SIP vessels Applikon service engineers are trained to make your old vessel not just new but turn it into a powerful yet intuitive industrial unit For a thorough inspection of your existing equipment call Applikon for an appointment and our service engineer will visit your facility After our Service Engineer details the specifications of
34. oven to be very useful in all industries that use anaerobic fermentations Because carbon dioxide production is stoichiometrically coupled with carbon source conversion very useful metabolic data can be obtained The AFM provides very accurate standard measuring of CO o g from six parallel fermentations that is both very low in maintenance and very easy to use Features e Very user friendly laboratory device for monitoring anaerobic metabolic yeast activity for alcohol production e Six independent yeast fermentations can be carried out simultaneously e Stirrer speeds and temperatures can be set or time programmed for each fermenter flask individually e Accurate comparison of conversion rates and yields under different conditions such as temperature strain type carbon source or nutrients e Much easier to operate and cleaner than standard fermenter systems e Fully controlled with user friendly PC control and data analysis software e Automatic generation of advanced reports containing all measured data data analysis tables and corresponding graphs e Very strong magnetic stirrers able to handle very viscous media e Proven value for research and QA purposes in all fields of yeast research and bio ethanol production Applications e Measure conversion rates and yields of lignocellulose hydrolysates into biofuels e Test and compare different yeast strains or different feedstock substrates e Quality control of regular commercial yeast e Qu
35. pe Belgium AppliTek N V Phone 32 9 386 3402 biotech applitek com Denmark Holm amp Holby A S Phone 45 43 269400 www Holm Halby dk France System C Industrie Phone 33 475548600 www system c industrie com Germany IUL Instruments GmbH Phone 49 222391920 www iul instruments de ye Spain Portugal Fisher Scientific Phone 34 902 239303 www es fishersci com Sweden AB Ninolab Phone 46 8 59086070 www ninolab se Switzerland ReseaChem GmbH Phone 41 344240310 www reseachem ch United Kingdom Applikon Biotechnology Phone 44 8453 373306 www applikon biotechnology co uk Ireland Asistec RID Technology Ltd Phone 353 0 1 2814650 2814064 www asistec e Russia Abacus Analytical System Phone 7 495 739 0363 www abacus lab com Poland Witko SP z 0 0 Phone 48 42 676 3435 www witko com pl Turkey Spektra Lab Laboratuvar Cihazlari Ltd Phone 90 312 447 77 09 www spektralab com Asia Singapore Zil Sci Pte Ltd Phone 65 6744 9943 www zilsci com India BioGentek BG PW Ltd Phone 91 11 5622255 5513104 www biogentek com Indonesia Pt Arico Sainsindo Nusantara Phone 62 2 1 5806342 www ptarico com China Hua Yue Enterprise Phone 86 2034821111 ext 8216 www huayueco com Applikon Biotechnology China Yuexiu District Guangzhou P R China Korea Jinyoung Industrial Co Phone 0082 1197517833 Australia amp New Zeeland
36. ply a combination of digital and analog outputs for process control Actuators such as rotameters solenoid valves mass flow controllers _ pumps thermocirculator and others can be controlled The operator can set P I D values dead band for pH control cascade control strategies and dose monitors for liquid additions The advanced auto tuning adaptive control system is part of every bundle and takes the guessing out of PID controller setting The system can automatically and continuously calculate the best controller settings for every process Classical pH and DO sensor port Gas in and Sample port Drain port Electrically cooled outlet lines condensor Accurate agitation Quick release topplate clamp PH and DO sensors Z Sample Agitator Withee tol or marine impellers Fluorophor pH and DO sensor port SS AppliFlex MiniBio g 2 Applikon Biotechnology Bioreactors Modularity and contigurability Although the BioBundle has a pre set configuration the whole bioreactor system remains modular and configurable With simple changes in configuration in the BioBundle system can be modified for different applications in a cost effective way This applies to changes in process control strategies in using extra gas or liquids but also in changing the system into a microbial set up Compact design The BioBundle is designed to occupy as small a footprint as possible
37. s Additional drivers available separately no no yes mE ADDA card ADDA card m Measurement of process values EM from process controllers yes yes yes m Control of process setpoints in mE process controllers no yes yes EE Starting and stopping of control loops no yes yes Programming of local process controllers no no yes mE Reading of calibration data no no yes mm 21 CFR part 11 compliant no no yes GAMP compliant yes yes yes ISA S88 standard no no yes Password protection no no yes User definable access rights no no yes Auto start after power failure no no yes BioXpert Lite BioXpert 2 BioXpert W7 mE 9 ciooikor A SV dU EAD tQ ciooikort Ar AHEAD 9 ciooikor AUR AHEAD BIOTECHNOLOGY BIOTECHNOLOGY BIOTECHNOLOGY Applikon Biotechnology Bioreactors 3 7 Specifications Specifications BioXpert Lite BioXpert 2 BioXpert W7 m Data management On line data collection yes yes yes EM Off line data collection yes yes yes mE Data storage system Proprietary file format Proprietary file format SQL database mm Minimum sampling frequency 1 minute 1 minute 1 second m Sample frequency selectable per parameter no no yes Data export to Excel yes yes yes mm Data export in ASCII format yes yes yes m Graph export in BMP format yes yes HENK On line calculations using on line and off line data no yes yes Data display Line graphs yes yes yes Bar graphs yes yes yes mum Synoptic yes yes yes m Procedure
38. seo acoustic cell retention system The Applikon BioSep system is a unique cell retention device for high density perfusion _ processes Using high frequency resonant ultrasonic waves to separate cells instead of NEN a physical mesh or membrane it offers all the benefits of traditional devices but without their inherent problems and limitations The BioSep based on the technology of acoustic EN resonance is a non fouling non clogging retention system The BioSep can be applied in both R amp D max 1L day process development and on production scale 1000L day m da X gii 2 s PTIT el ea A A Er m amm EN IT m anm D LT IT angu Snr gt A a A a m oo ED 5 u u m m m mu m oum m mu m m m m m us m m m m m u m HM M o OM o EUM UM UM UM HM SD HM UM EM HM UM UM UM um Um Applikon Biotechnology Bioreactors A Typical configuration of acoustic cell retention system CLARIFIED CULTURE MEDIUM A FRESH FEED Features e Proven under cGMP conditions e Easy to install and to operate HARVEST CONCENTRATED CELL RECYCLE CELL SUSPENSION CELL SETTLING Applications e Cell cultures e Perfusion cultures e Can be used in combination with any brand of bioreactor e ong term cultivation possible 290 days e No fouling or blocking e No damage to the cells e High separation efficiency 29596 Specifications BioSep sy
39. single wall 15 liter jacketed 20 liter single wall 1 liter 2 liter single wall 2 liter jacketed 3 liter single wall 3 liter jacketed 5 liter single wall 5 liter jacketed 7 liter single wall 7 liter jacketed 15 liter single wall 15 liter jacketed 20 liter single wall Drive system Maximum stirrer speed rpm Impellers Gas sparger Gas overlay Exhaust gas Sampling Draining Additions pH DO Temperature Foam Level Optional inlets Applikon Biotechnology Bioreactors 1 h Total Working Minimum Aspect Aspect volume volume working ratio total ratio working L L volume L volume L volume L 1 25 0 9 0 3 2 1 129 22 1 7 0 5 72 1 9 22 17 0 5 2 2 1 9 3 1 ZA 0 5 1 9 1 3 1 2 7 05 1 9 19 4 8 3 4 0 9 1 6 1 1 4 8 3 4 0 9 1 6 E 6 8 5 4 1 5 2 2 128 6 8 5 4 Io 2 2 18 18 5 12 3 0 lof 1 9 18 2 12 3 0 149 1 2 23 4 16 3 0 2 4 2 0 Internal Diameter mm Internal Height mm Autoclave dimensions WxH mm 95 200 90172 x 393 105 240 9190 x 436 105 240 9219 x 486 130 240 9190 x 436 130 240 0235 x 436 160 250 9260 x 436 160 250 9260 x 480 160 350 9260 x 600 160 350 9264 x 645 222 440 2381 x 710 240 440 0391 x 740 222 620 381 x 900 Direct drive lipsealed or magnetically coupled Standard range is 50 1250 1 2 and 3 liter systems can be supplied with 2000 rpm motor Rushton and marine with outside diameters 45mm 60 mm 75 mm or 85 mm Porous sparger or L type sparger Yes Water cooled exhaust gas co
40. stem 1L 10L 50L 200L 1000L Maximum perfusion rate 1 L day 7 L day 45 L day 200 L day 1000 L day Minimum perfusion rate 0 1 L day 0 7 L day 4 5 L day 20 L day 100 L day Separation efficiency Up to 99 depending on cell concentration and perfusion flow Minimum cell concentration 2 x 105 cells ml depending on cell size Bioreactor connection 6 mm OD tube 12mm OD tube Mounted Mounted Mounted separately separately separately Medium inlet 3 mm hosebarb 6 mm hosebarb 10 mm hosebarb 0 5 TC 05 TC Return line to bioreactor 3 mm hosebarb 6 mm OD 0 5 TG 0 5 TC 0 5 TC diptube Perfusion outlet 3 mm hosebarb 6 mm hosebarb 6 mm hosebarb 0 5 TC 0 5 TC Weight 0 1 kg 0 5 kg 1 5 kg 13 kg 70 kg Resonator volume 0 7 ml 7 ml 50 ml 290 ml 1450 ml BioSep Controller APS 995 APS 990 APS 990 APS 992 APS 993 BioSep Controller Power 15 Watt 150 Watt 150 Watt 350 Watt 1500 Watt BioSep Controller Dimensions Combined with 305x130x130 305x130x130 450x400x135 450x300x450 DxWxH mm my Control BioSep Controller Weight kg Combined with 3 5 3 5 17 6 43 my Control e Applikon Biotechnology Bioreactors Bencn scale ana Pilot plant steam in place bioreactor systems Applikon s concept of moaularity using standard modules to customize the functions of the bioreactor is extended to the stainless steel pilot plant bioreactors as well For scale up purposes the range of the Bio Bench and Pilot Systems designed and bullt to the latest standards on hygieni
41. sy integration of the ez Controller into your network Emergency stop switches the system to safe position in case of emergency Connections for USB connection pH and DO TFT color touch screen interface for Temperature and l UN Foam Level High precision easy navigation motor and clear data display Motor and Encoder motor Optional ADDA pH dissolved card for gt oxygen and Gas mixing and additional 1 0 temperature easy gas HENK sensors selection system Connections for Easy access headplate Up to 4 gas rotameters and up to 4 mass flow controllers for flexible gas supply User friendly detachable and autoclavable bottle rack accommodates up to 3 liguid addition bottles D000 Ko 8 BARLETE TEY Temperature section for thermocirculator cooling water valve or heating blanket Quick release connections for condensor and heat exchanger Pump section with up to 3 easy access fixed speed pumps for controlled liquid addition Autoclavable glass bioreactor ez Control Applikon Biotechnology Bioreactors 21 combination of ease of use and sophistication reducing the time to start up a process The system is equipped with the intelligent and powerful process controller easy and intuitive to operate such that the user manual might not be needed The process controller has control loops for pH Temperature Dissolved Oxygen Foam Level and agitation and can ap
42. ted to the Applikon bioreactors to tum your standard bioreactor into a photobioreactor with no downtime and minimal investment NN Features e Proven bioreactor design e Photo synthetically Active Radiation PAR e ong lifetime 100 000 hrs in combination with 400 700nm and Ideal for plant growth to achieve good lumen maintenance better efficiency in the response of plants e ow voltage operation having more than 9096 e Excellent wavelength stability over time efficiency with no EMI radiation consistent high reliability e Directional light output amp Even distribution of light e Usable wavelength blue 470nm and red 660nm over the target application area for plant growth e Great performance in cold environments e Fully regulated form O to 100 light intensities e No heat generation O to 20 000 lux in real time Applications e he PhotoBio can be used for all photosynthesizing organisms plants algae bacteria Specifications Maximum driving capacity Programmable zones Intensity control Time base Time range Light driving Housing amp Protection Class User interface Serial Communication port Ambient temperature Power supply Power consumption 140 watt of power or 24 light bank of 6 watt 8 O to 100 PWM Built in Real time clock 99 59 hours minutes per step maximum 9 time intensity steps in one cycle Constant current source IP 30 and IP 65 for Control Panel And
43. tion with alkali pump variable speed pump Measurement 6 mm classic polarographic DOs sensor Control via a combination of No Air Oo optional MFC and agitation or nutrient addition variable speed pump Measurement Pt 100 sensor in thermowell in topplate Control electrical cooling and heating jacket via bioreactor wall Measurement Height adjustable conductivity based foam sensor Control Anti foam addition variable speed pump Measurement Height adjustable conductivity based level sensor Control variable speed pump for liquid addition or removal Septum chemostat tube liquid entry system Biomass Optical Density Oo and CO off gases Applikon Biotechnology Bioreactors 1 gt Glass autoclavable bioreactors u the world wide standard In the laboratory bioreactor and fermentor range Applikon is worldwide market leader because of its dependable and easy to use systems The bioreactors and fermentors excel in quality and modularity An Applikon laboratory system is easy to upgrade if a change in research activities occurs Applikon offers glass autoclavable bioreactors for cell culture applications and glass autoclavable fermentors for microbial culture applications The systems are built according to the specific demands of a process using an extensive array of standard components Because of the modularity and flexibility the user can always adapt the systems to changed process demands This results i
44. tor cooling via Peltier element Flectrical condenser cooling via Peltier element Spare I O 8 x digital output 4 x analog out 0 4 20 mA Additional features Emergenoy stop connection central alarm output System status indication inactive active alarm by integrated colored light Power Pmax 480 VA 1 19 2 2910 Vac Dimensions d x w x h 200 x 190 x400 Mm Do Vol Weight kg 8 5 kg 26 Applikon Biotechnology Bioreactors in Control simply powerful in Control is a process controller for laboratory scale bioreactors The system is a drop in replacement for our older ADI 1010 Bio Controller and ADI 1030 Bio Controller The in Control offers high level control on a small footprint When you are running your bioreactors controlled by our ADI 1010 or ADI 1030 Bio Controllers the in Control can be used to increase the capabilities of your bioreactor system with a minimal investment and no downtime for your process he controller can be used for both cell culture and microbial cultures The intuitive human interface is a built in color touch screen Features e Control of all bioprocess parameters including pH temperature dissolved oxygen agitation foam and level e Selectable autotuning adaptive PID control for accurate control when process conditions change during the culture e Easy operation through touch screen interface and through web browsers Windows PC Apple PC Linux PC iPhone iPad Android tablet
45. trol via liquid alkali pump and COs gas supply Measurement via Pt 100 Heating only via heating blanket Measurement via DO sensor control via Air and Oo gas supply Option Option 3 ports in triple inlet and one inoculum port One liquid storage system included Included Included Applikon Biotechnology Bioreactors 2 a BioBundle Microbial ez Control 16 26 SL Ok TL 15L 20L O 9L 1 7L 2 7L 3 2L 5 4L 12L 16L Lipsealed with Rushton impellers Air and Oxygen supply via sparger and overlay Gas outlet condenser Sample pipe included Sample system optional Measurement via pH sensor control via liquid alkali and acid addition pump Measurement via Pt 100 Heating via heating blanket cooling by cold water in heat exchanger Measurement via DO sensor control via Air and Oo supply and agitation speed Measurement via Foam sensor control via anti foam addition pump 3 ports in triple inlet one inoculum port 1 and septum port Three liquid storage systems included Included Included Appliflex BioBundle ez Control 10L 20L 50L oL 10L 25L Mixing by rocking motion Air and Oxygen supply via overlay condenser Sample line included Sample system optional Measurement via pH sensor control via liquid alkali pump and COs gas supply Measurement via Pt 100 Heating only via heating blanket Measurement via DO sensor control via Air and Oo gas supp
46. ur DO sensor while autoclaving your bioreactor system resulting in reduced start up time for your culture Features Applications e Titanium and PEEK materials guarantees low drift e Microbial cultures e cGMP compatible by using FDA approved materials e Cell cultures e Electropolished surface roughness finish of 0 4 um e Batch eases cleaning e Fed Batch e Short response time e Continuous cultures e Withstands repeated SIP and CIP cycles e Perfusion cultures e Standardized PG 13 5 connection guarantees interchangeability with other bioreactor brands wv Q un r t A a o lt 7 ApptiSens Specifications Optimum polarization potential DOT AA LJ es d CALAS um LY Ww 800 mV Q 40 C Polarization potential amplifier 675 mV max Pressure where linearity is guaranteed 4 bar max Sterilization temperature 135 C minimum polarization time 30 minutes minimum polarization time after autoclaving 4h optimum polarization time after autoclaving Overnight Polarization current Polarization current Polarization current Air 25 C 1 013 bar 100 RH Air 40 C 1 013 bar 100 RH O2 40 C 1 013 bar 096 RH Response time gas phase 20 C Response time gas phase 40 C Response time gas phase 60 C t 9096 t 9096 t 9096 Drift between 15h 5 days Drift between 5 days and 7 days Drift between 7 days and 30 days 0 2 96 day 0 1 96 day 0 05 96 day

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