Master Cover Design
Contents
1. CS ANN DD EE al 3 4 Adjusting eyepiece diopter 1 Rotate the 10x objective into position 2 Rotate the diopter rings on the eyepiece tubes until its numerical value is the same as your interpupillary distance for example 70 in the figure See Fig 6 3 Close your left eye and bring the specimen into focus following the focusing procedures in 3 5 4 Close your right eye and bring the same specimen into clear sharp focus by adjusting the diopter ring on left eyepiece tube only Do not use focus knobs at this step 5 Since both sides are adjustable you may also do the above in the opposite way in other words left eye first and right eye second OMAX www microscopenet com 3 5 Focusing 5 With the 10x objective in position raise the mechanical stage using the coarse focus knob until the specimen is close to the objective Turn the coarse focus knob until the specimen is in focus Use the fine focus knob to obtain a sharp image To get a good focused image you may need to combine the focus knob adjustment and interpupillary distance adjustment along with eyepiece diopter adjustment stated in 3 3 and 3 4 You may now switch to another magnification objective Focus Stop Lever Fig 7 Tips To prevent your specimen slide from making contact with an objective raise the stage to its highest position without contacting the 100x objective then tighten the focus stop lever Fig 7 Give the stage2. a E Viewing Head w Camera Head Thumb Lock Screw Microscope Body Focus Tension Ring Coarse Focus Knob Fine Focus Knob X Y Stage Moving Knobs USB Cable Brightness Intensity Dial E e www microscopenet com E w 4 x v PA F 10 11 12 13 14 15 16 ase ey fy 18 N A 19 23 24 29 N Power Switch Condenser Lock Thumb Knob Condenser Focus Knob Focus Stop Lever Abbe Condenser Color Filter Holder Iris Diaphragm Lever OMAX www microscopenet com 2 Installation 2 1 Installation of the binocular viewing head 1 Loosen the head thumb lock screw on the top of the microscope body and remove the plastic cover on the top 2 Remove the cap on the dovetail of the binocular viewing head 3 Seat the dovetail completely of the viewing head into the socket on the top of the microscope body and tighten the head thumb lock screw Caution Do not release the viewing head from your hand grip until you are sure the viewing head is installed securely 2 2 Installation of the eyepieces 1 Remove the protective caps from the eyepiece tubes 2 Insert the eyepieces into the eyepiece tubes 2 3 Installation of the objectives 1 Adjust the coarse focus knob until the mechanical stage is at its lowest position 2 Turn the caps counter clockwise to remove them from the nosepiece 3 Take the objectives out from the plastic cases and turn each o
3. 4 If any specimen solutions or other liquids splash onto the stage objective or any other component disconnect the power cord immediately and wipe up the spillage Otherwise the instrument may be damaged 5 Important the lamp lamp housing and adjacent parts will become very hot Do not touch these parts until they have completely cooled Never attempt to handle a hot halogen bulb 6 All electrical connectors power cord should be inserted into an electrical surge Suppressor to prevent damage due to voltage fluctuations 7 Confirm that the input voltage indicated on your microscope corresponds to your line voltage The use of a different input voltage other than that as indicated will cause severe damage to the microscope OMAX www microscopenet com ii Care and Maintenance 1 Do not attempt to disassemble any component including eyepieces objectives or focusing assembly 2 Keep the instrument clean remove dirt and debris regularly Accumulated dirt on metal surfaces should be cleaned with a damp cloth More persistent dirt should be removed using a mild soap solution Do not use organic solvents for cleansing 3 The outer surface of the optics should be inspected and cleaned periodically using an air stream from an air bulb If dirt remains on the optical surface use a soft cloth or cotton swab dampened with a lens cleaning solution available at camera stores All optical lenses should be swabbed using a circular moti
4. NA 1 25 11 Plan 100X 1 25 oil 0 5 160 0 17 spring w iris www microscopenet com diaphragm Annular Ring Plates five positions 10 for 10X phase contrast objective 20 for 20X phase contrast objective 40 for 40X phase contrast objective 100 for 100X phase contrast objective B for bright field observation with iris diaphragm five positions 10 for 10X phase contrast objective 20 for 20X phase contrast objective 40 for 40X phase contrast objective 100 for 100X phase contrast objective B for bright field observation with iris diaphragm 1 For 10X phase contrast objective 1 For 40X phase contrast objective 1 for 100x phase contrast objective Mounting Size diameter Centering Telescope focusing adjustable OMA X www microscopenet com 6 Troubleshooting Guide GENERAL PROBLEMS protien eee Seton No electrical power Check power cord connection Lamp does not light Lamp bulb burnt out Replace bulb when switched on Fuse blown out Replace fuse Revolve the nosepiece to click stop position by swinging the objective correctly into the optical path The light source of the bulb is not at Adjust the position of the bulb the center Dirt or dust on the lens eyepiece condenser objective collector lens or specimen No slide cover attached to the slide Attach a 0 17mm slide cover Use a slide cover of the appropriate thickness 0 17mm Slide may be upside down Turn slide
5. a tiny extra moving space to ensure the objective can be focused every time 3 6 Applying the immersion oil 1 Rotate the objective nosepiece to seat the observing position between the 40X and 100X objectives as shown in Fig 8 a 2 Place a drop of immersion oil on the slide cover as shown in Fig 8 b 3 Rotate the objective nosepiece to seat the 100X objective to the observing position until you hear a click sound 4 After observing the specimen use the camera cleaning kit to clean the 100X objective lens gently and the specimen in time 5 If itis hard to clean you need a little bit alcohol to clean the 100X objective lens and the specimen Fig 8 Caution important When you use the 100X objective to observe the specimen you have to finish observing the specimen with the 4X 10X 40X objectives When you use the 100X objective to observe the specimen you have to apply the immersion oil on the top of the slide cover When you apply the immersion oil with the 100X objective do not let the immersion oil to contact with the dry objective lenses especially the 40x If the immersion oil is on the dry objectives lens please use the camera cleaning kit to clean the objectives lens in time The oil will damage the dry objective lenses After observing the specimen with the 100X objective clean the 100X objective lens first _Q OMAX www microscopenet com 3 Adjusting condenser 1 Turn the condenser
6. d under the objective then you will see the dark ring Fig 22 as shown in Fig 22 a lf the bright ring is still obscure as in Fig 22 b raise or lower the condenser by adjusting the condenser focusing knob or the microscope focusing knob if necessary till the bright ring is in focus and the dark ring is visible lf the two ring images are not coincided as shown in Fig 22 c adjust the two centering screws on the condenser ring plate till the two rings are coincided with each other as shown in Fig 22 d Remove the centering telescope and replace it with the eyepiece following the procedures from 2 b to 2 h Fig 23 Tips e Make the illumination as bright as possible e The thinner the specimen the better the image
7. focus knob to raise or lower the condenser 2 Raise the condenser when using high power objectives and lower it when using low power objectives Note e The centering of the condenser and the light axis of the objective are factory adjusted Do not attempt to re adjust e The highest position of the condenser has been factory adjusted Do not attempt to re adjust 3 8 Adjusting iris aperture diaphragm Swing the iris diaphragm lever Fig 9 left or right to adjust the aperture size Note The iris diaphragm is designed to adjust the aperture size not to adjust the brightness although the brightness will be changed when it s adjusted When aperture is adjusted to smaller size the contrast will be increased and the depth of field will be increased as well Turn up the intensity of the light if the image is too dim 3 9 Adjusting focus tension The focus tension has been pre set at the factory If the mechanical stage drops by itself rotate the tension adjustment ring Fig 10 situated between the coarse focus knob and microscope body on the power switch side until the tension is in maintained 3 10 Photo video observing capturing and recording 1 Insert the USB cable into the USB port Fig 11 on the back of viewing head and the other end to the computer 2 Turn on the computer install the camera following the manual in the mini CD 3 Launch the observing software to examine 4 You also can capture images or rec
8. 191BOIL only Make sure the oil contact the bottom of the slide and the top of the condenser during the adjustment ee OMAX www microscopenet com 7 Darkfield Condenser Installation and Operation Instructions Condenser Holder 7 1 Dry darkfield condenser of A191 jconsenses Lock Thumb Screw 1 Mounting the dry darkfield condenser e Loosen the condenser lock thumb screw on the condenser holder and remove the brighitfield condenser e Install the dry darkfield condenser and tighten the condenser lock thumb screw on the condenser holder see Fig 12 2 Centering the dry darkfield condenser e Turn the 4X objective to the light path e Turn the condenser focus knob to lower the condenser till a dark spot showed in the viewing field as shown in Fig 13 a e Turn the condenser translational centering screws to move the dark spot to the center as shown in Fig 13 b 3 Place the slide on the stage 4 Raise the condenser all the way to the top and lower it a little bit 5 Following the procedures in this manual to focus and observe 6 a Move the condenser up or down slightly to get the best darkfield viewing Fig 13 P Note e The dry darkfield condenser is used with the dry objectives only e The dry darkfield condenser works with the 4X 10X 40X objectives e The dry darkfield condenser won t work with the 100X oil immersion objective j i Cond Hold 7 2 Oil darkfield condenser of A191BOIL gt Condense
9. CD Compatible with Windows 2000 XP Vista and Windows7 32 64 bit Darkfield Condensers optional Refer to the Darkfield Condensers specifications Phase Contrast Kits optional Refer to the Phase Contrast kits specifications Camera N ight 10 4 75 k Carrying Case optional er elds Te Or ane Size 16 1 2 x 12 1 2 x 13 3 8 42cm x 32cm x 34cm Illumination Transmitted 6V 20W Halogen Variable intensity Power Supply AC 100V 240V 50 60HZ US and Canada plug Dimension 7 118 x 10 1 4 x 15 3 8 20cm x 26cm x 39 cm Net weight 12 lbs 5 45 kg 10 5 Optional Parts The optional parts may be included in some models or sold separately 1 Darkfield Condensers A191 i A191BOIL oo 1 36 1 25 Darkfield Condenser 2 Phase Contrast Kits A1PHB1 A1PHB3 Phase Contrast Objective Plan achromatic 10X with built in phase plate Plan achromatic 20X with built in phase plate Plan achromatic 40X with built in phase plate spring Plan achromatic 100X with built in phase plate spring oil Achromatic 10X with built in phase plate Achromatic 20X with built in phase plate Achromatic 40X with built in phase plate spring Achromatic 100X with built in phase plate spring oil Achromatic 10X with built in phase plate Achromatic 40X with built in phase plate spring Achromatic 100X with built in phase plate spring oil Numerical Aperture ondenser
10. User Manual Digital Binocular Compound Microscope Model MD827S30 series MicroscopeNet com Table of Contents PS UIC IN esere ee ace doe wake nce tne naseenec ETER 1 li Care and Maintenance cccccce cece cece eee ee a 2 1 Component INlUStration cece RE AA OROS 3 2 MAST ANIAUIOM setaerst acca eats ito vnswaaonedaneatetedanneheatenel ceeareieatoreees 4 ZOPE 11 0 Mee mere nee eee eee a eee 7 AS DCCINCAUONS coiir mac ebiinwexdasmadad mawsetecs ramen eines 10 5s ODUON al PANS vrcence cacensmcreenenncehesepaanonaesanscadnenaanidesseasaeaaaeress 11 FOUDIESNHOOUNG GUIGC is ceccesazs secre Sa 12 7 Darkfield Condenser Installation and Operation Instructions 14 Phase Contrast Kit Installation and Operation Instructions 15 OMAX www microscopenet com i Caution 1 Open the carton carefully with a knife or paper cutter Find the UP sign and place the Styrofoam container on the side that makes the arrow upward If the UP sign is missing please open the Styrofoam container gently to prevent any accessory i e objectives or eyepieces from dropping and being damaged 2 Do not discard the molded Styrofoam container The container should be retained should the microscope ever requires reshipment 3 Keep the instrument out of direct sunlight high temperature or humidity and dusty environments Ensure that the microscope is located on a smooth level and firm surface
11. ed and even brightness See Fig 3 6 Screw the cover on a Halogen Bulb Screws Fig 3 Caution Before you turn over the microscope be sure to take the eyepieces off and be certain that the head is securely locked by the thumb screw 5x OMAX www microscopenet com 2 6 Replacing the fuse 1 Turn off the power switch and disconnect the power cord 2 Turnover the microscope on its side find the fuse holder at the bottom of the base 3 Turn the fuse holder counter clockwise to take it off replace the fuse and then turn it on clockwise See Fig 4 ee x ere wa ie ja ar agl ps AF a gt T A ee ENET AN Bh ed i Pa Fuse Holder Fig 4 Caution Before you turn over the microscope be sure to take the eyepieces off and be certain that the head is securely locked by the thumb screw 2 Installing the mirror optional may not included in your package 1 Turn off the power switch and disconnect the power cord 2 Screw off the light collector on the microscope base 3 Screw the black disc onto the base and then insert the mirror into the hole at the center of the black disc See Fig 5 You may try to get reflected ambient light on either side of the mirror with different angles for best result Light Collector Fig 5 Note The mirror is only used when there is a power failure or you are in the field and no power is available 2 8 Installation of the darkfield cond
12. ensers optional may not included in your package Please see section 7 Darkfield Condenser Installation and Operation Instructions 2 9 Installation of the phase contrast kits optional may not included in your package Please see section 8 Phase Contrast Kit Installation and Operation Instructions OMAX www microscopenet com 3 Operation 3 1 Adjusting illumination 1 Plug the power cord into the power socket on the microscope and connect it to the power outlet 2 Turn on the power switch 3 Rotate the brightness intensity dial to increase or decrease the brightness Caution A diffusion filter is attached beneath the condenser to get uniform light and protect your eyes from strong light when a low power objective applied The diffusion filter can be swung out to make the view field brighter when observing with a high power objective such as 100X objective 3 2 Placing specimen 1 Place the slide on the mechanical stage 2 Use the slide holder to gently secure the slide 3 Turn the X and Y stage moving knobs to position the specimen in the center of viewing field Caution Be sure not to allow an objective to touch a specimen slide when changing objectives 3 3 Adjusting interpupillary distance While observing with both eyes hold the left and right eyepiece tubes then slide the tubes in and out The interpupillary distance is correct when the left and right fields of view converge completely into one image
13. ne clock wise into the holes on the nosepiece Install the 4X objective into the nosepiece first Then ina counter clockwise direction rotate the nosepiece and install each succeeding higher magnification objective as shown in Fig 1 Ps es i I A HAW NWI ANNI a 144 MMIII iiil UI Wit ee al po Fig 1 e Inspect the objectives frequently for dirt or oil clean if necessary e Use the 10X objective to initially focus the image of your specimen e When changing the objective magnification rotate the objective nosepiece until you hear a click sound or have a clear in position feeling This ensures the objective is centered in the optical light path OMA X www microscopenet com 2 4 Installation of the glass Filter 1 Swing out the color filter holder under the condenser 2 Place the filter into the holder as shown in Fig 2 swing the holder in Color Filter Holder Fig 2 2 5 Installing or changing the halogen bulb 1 Turn off the power switch and disconnect the power cord 2 Allow some time to cool down the lamp Turn over the microscope on its side find the bulb compartment at the bottom Open the cover of the bulb compartment by loosening the thumb screw Take out the dead halogen bulb and insert the new halogen bulb Be sure the pins on the bulb are completely inserted into the lamp socket You may also loosen the two screws on the cover to adjust the position of the bulb to get center
14. on A small amount of absorbent cotton wound on the end of a tapered stick makes a useful tool for cleaning recessed optical surfaces Avoid using an excessive amount of solvents as this may cause problems with optical coatings or cemented optics or the flowing solvent may pick up grease making cleaning more difficult Oil immersion objectives should be cleaned immediately after use by removing the oil with lens tissue or a clean soft cloth 4 Observe the specimen with the 4X 10X and 40X objectives in order then observe the specimen with the 100X objective Apply the immersion oil on the slide cover with the 100X objective Do not let the immersion oil to contact with the dry objectives lens especially the 40X Clean the dry objective lens using the camera cleaning kit if the immersion oil is on the dry objectives lens Clean the 100X objective lens first using the camera cleaning kit after observing the specimen with the 100X objective then clean the specimen More persistent dirt should be removed using a little bit alcohol Do not use organic solvents for cleansing 5 Store the instrument in a cool dry environment Cover the microscope with the dust cover when not in use 1 Components Illustration O ON OA1FRWDND 20 21 22 Eyepiece Diopter Ring Eyepiece Tube Nosepiece Objective Slide Holder Mechanical Stage Light Collector Microscope Base 10 11 12 13 14 15 16 17 18 ty S AA Ba
15. openet com 5 Centering the annular ring a b 8 2 Phase contrast kit of A1PHD 1 Mounting the phase contrast kit Note Remove one eyepiece from the microscope eyepiece tube and insert the centering telescope as shown in Fig 18 Observe from the telescope The bright ring and dark ring should be coincided with each other as shown in Fig 19 d If the ring images are not clear turn the top of telescope until both ring images are in focus lf the bright ring is still obscure as in Fig 19 b adjust the condenser focus knob lf the two ring images are not coincided as shown in Fig 19 c hold the ring plate from the bottom of the annular ring disk and adjust its position until two ring images are coincided Remove the centering telescope and replace it with the eyepiece Put the specimen on the stage and adjust the illumination focusing etc following the instructions in this manual e The phase contrast condenser will be working as a conventional Abbe condenser if the annular ring disk being put at B position a Replace the bright field objective s on nosepiece with the phase contrast objective s b Thread the condenser ring plate onto the condenser as shown in Fig 20 c Loosen the condenser lock thumb screw as shown in Fig 21 take off the original condenser from the holder d Insert the phase contrast condenser into the condenser holder as shown in Fig 27 tighten the condenser lock thumb sc
16. ord live videos through the software Note For the details of installation and operation of the camera and its software please refer to the manual in the mini CD Fig 11 3 11 Darkfield condensers observation optional may not included in your package Please see section 7 Darkfield Condenser Installation and Operation Instructions 3 12 Phase contrast kits observation optional may not included in your package Please see section 8 Phase Contrast Kit Installation and Operation Instructions OMAX www microscopenet com 4 Specifications Model MD827S30 series 40X 80X 100X 200X 400X 800X 1000X 2000X Total Magnification Viewing Head Binocular 45 inclined 360 swiveling w built in camera Interpupillary Distance Sliding adjustment 55mm 75mm Diopter Adjustment On both eyepiece tubes 1 pair of WF10X 18 1 pair of WF20X Eyepieces Objective Tube Length 160mm Nosepiece Revolving quadruple Objectives Achromatic DIN 4X 10X 40X spring 100X spring oil Abbe NA 1 25 w iris diaphragm and filter holder Rack and pinion adjustment Condenser Focus Mechanism Coaxial coarse and fine focusing knobs on both sides w focus stop Minimum fine focusing adjustment at 0 002mm range 28mm Mechanical Stage Double layer Dimension 5 1 2 x 5 1 2 140mmx140mm Translational range 3 x 2 75mm X 50mm Built in USB2 0 2048 x 1536 pixel 3 0MP Driver and Software included in the
17. ork well if no oil drop applied on the condenser 8 Phase Contrast Kit Installation and Operation Instructions 8 1 Phase contrast kits of A1PHB1 or A1PHB3 1 Mounting the phase contrast objectives a Take off all the objectives from the nosepiece b Install the phase contrast objectives onto the nosepiece following the steps in 2 3 2 Mounting the phase contrast condenser annular ring disk a Rotate the nosepiece and set the 4x objective in position b Turn the coarse focus knob to raise the mechanical stage to the highest position without contacting the 4X objective c Turn the condenser control knob to raise the condenser to the highest position d Loosen the light collector and take it off e Loosen the condenser lock thumb screw pull down the condenser and take it off f Insert the condenser annular ring disk into the condenser holder and tighten the thumb screw g Insert the light collector back onto the base and turn until it s tightened h Re adjust the condenser height to its normal position i Re adjust the mechanical stage height to its normal position Note e When raising the mechanical stage do not make contact with the objective 3 Turn the desired objective into light path 4 Turn the annular ring disk to put the corresponding ring into light path i e if you are using the 40x phase contrast objective you should turn the disk at 40 as shown in Fig 17 Fig 17 _15 OMAX www microsc
18. osition Adjust the iris diaphragm ring on the It s totally dark in objective the viewing field Lower or raise the condenser slightly to the position that the specimen is the brightest The condenser is not in the right position A191BOIL only Make sure the oil contact the bottom of the slide and the top of the condenser during the adjustment FrGAnienciy ORlameieico lew Adjust the intensity dial to increase the y p brightness Lower or raise the condenser slightly to the position that the specimen is the brightest The condenser is not in the right position A191BOIL only Make sure the oil contact the bottom of the slide and the top of the condenser during the adjustment The condenser is not centered Adjust the translational centering screws to properly center the condenser A191BOIL only The built in iris diaphragm of 100X objective is not in proper position The specimen is not suitable for ae darkfield observation Change to brightfield or phase contrast A191BOIL only The built in iris Image of the diaphragm of 100X objective is not specimen is not in proper position clear and lacking in sufficient contrast The illumination is Insufficient or too bright Turn the iris diaphragm ring on the objective Turn the iris diaphragm ring on the objective Lower or raise the condenser slightly to the position that the specimen is the brightest The condenser is not in the right position A
19. over so the cover glass Specimen at the bottom faces up Diopter adjustment is not set Readjust the diopter settings properly Immersion oil is on a dry objective Check the objectives clean if especially the 40x necessary Poor image quality No immersion oil used with 100x ice mmercioncl or not able to get objective focused image Air bubbles in immersion oil Remove bubbles Condenser aperture is closed or Open or close properly open too much Condenser is positioned too low Position the condenser upward Secure the specimen in the slide Specimen rises from stage surface holder Blue filter not used Use daylight blue filter Lamp intensity is too high or low Adjust the light intensity by rotating the intensity control dial _12 Darkness at the Revolving nosepiece not in click stop periphery or uneven brightness in the field of view position Dirt or dust on the view Clean the lens with a camera cleaning kit Slide cover is too thick or thin www microscopenet com neg coarse focusing knob Fine focus is Tension adjustment is set too high Loosen the tension on the focusing ineffective knobs DARKFIELD PROBLEMS ee The light is not on Turn on the light A191BOIL only There is no oil in Place a drop of oil on the top lens of between the condenser top lens condenser and let it contact the underside and slide of slide A191BOIL only The built in iris diaphragm of 100X objective is not in proper p
20. r Lock Scns o bist Screw L Centering Screws Fig 12 1 Mounting the oil darkfield condensers e Loosen the condenser lock thumb screw on the condenser holder and remove the brightfield condenser e Install the oil darkfield condensers and tighten the condenser lock thumb screw on the condenser holder Centering Screws see Fig 14 Fig 14 2 Centering the oil darkfield condensers e Turn the 40X objective to the light path e Turn the condenser focus knob to slowly lower and raise the condenser till a dark spot showed in the viewing field as shown in Fig 15 a e Turn the condenser translational centering screws to move the dark spot to the center as shown in Fig 15 b Fig 15 14 OMAX www microscopenet com 3 Raise the condenser till the top lens is close to the opening of stage Place a drop of immersion oil on the top of the lens of the condenser 4 Place the slide on the stage Raise the condenser and let the oil drop contact the bottom of the slide If air bubbles exist in the oil clean the oil from the condenser lens and bottom of slide with a camera cleaning paper and repeat the procedures 5 Follow the procedures in this manual to bring the sample on slide in focus and observe 6 When using the 100X oil darkfield objective you will need to drop the immersion oil and adjust the iris ring Fig 16 to get proper brightness and contrast of view field Note e he condenser won t w
21. rew 7 z eam a ea _ M p 10X Fig 21 16 OMA X www microscopenet com Note there are 3 phase contrast objectives 10X 40X and 100X and there are 3 condenser ring plates 10X 40X and 100X The corresponding objective and ring plate must work together i e 10X phase contrast objective must work with 10X condenser ring plate and so on 2 Centering the condenser ring plate a b C j 3 Centering the condenser ring plate After you center the ring plate you can perform the phase contrast observation the same way as a normal bright field microscope Note when change to another phase contrast objective and corresponding condenser ring plate the focusing and centering of bright ring and dark ring should be repeated Connect the power cord to the microscope and insert the plug into a power outlet Turn the desired phase contrast objective into light path Choose the ring plate that corresponding to the phase contrast objective in light path and screw it on the condenser Replace the bright field condenser with the phase contrast condenser with ring plate on Remove one eyepiece from the microscope eyepiece tube and insert the centering telescope Turn the light of microscope on and observe from the telescope Turn the top of the telescope Fig 23 until the dark ring image is in focus If the dark ring is hard to find LE Tana put a normal white print paper on the stage and c
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