User Manual - Spectra Services
Contents
1. 10 INDICATOR MIPS gt tT SL 1750735 BAG 5 SENSOR SL 200020 HUMIDITY iss 5 SENSOR ALARM CONTACTS 4 5 HUMIDITY HUMIDITY SENSOR DISPLAY SL 4100500 10W SL 1750736 CO2 INDICATOR 51 200021 ALARM JACK se DOOR HEATERS SL 100163 A SL 103068 DATA LOGGER IR CO2 SENSOR SL 1750737 SL 8320510 47uf DOOR SWITCH SL X1000022 CO2 SOLENOI N SL 8600528 SL LOGO LIGHT BOARD RED SL 1750737 12VDC POWER SUPPLY SL 6750507 CIRCULATING FAN MOTOR SL 4880508 26 WIRING DIAGRAM SCO5W 2 3517 2 SCO10W 2 3524 2 230V FUSED POWER INLET 6A SL 104179 FUSE HOLDER SL 3300501 GREEN CHERRY a a I a T e T ROUND pea ae Sere ee ee POWER SWITCH SL 7850532 BLU T WHITE y WHEE WATER FILL WATER FILL INDICATOR SWITCH SL 210004 SL 200020 CO2 PREHEATER SL 103328 BLAGK 6w USB BOARD SL 1750846 10 EARTH GRD COMMUNICATION USB EXSTENSION CABLE SL 1800533 HEATING INDICATOR 51 200021 a TEMP D HOT o A m DISPLAY S 914 1750734 4 ORANGE OTP SL 100001 gt m LOAD 1 a 2 2 MAIN TEMP s ORANGE SENSOR 555 MAIN HEATING ELEMENT z2. 0 4 LOOP CONTROL SL 120071 2 BOARD 51 1750725 OTP INDICATOR w DISPLAY O foz 10803 SL 200020 2 SL 1750735 5 SENSOR Cz HUMIDITY W Q SENSOR ALARM CONTACTS 4 py TOW O F 0 WH A HUMIDITY HUMIDITY SENSOR DISPLAY SL 4100500 Tow SL 1750736 2 INDICATOR SL 200021 TOW ALARM JACK DOOR HEATERS SL 100163 SL 103068 A7uf DATA LOGGER IR CO2 SENSOR SL 1750737 SL 8320510 A CO2 SOLENOI DOOR SWITCH SL 8600529 SL X1000022 SL LOGO 12VDC LIGHT BOARD BEAG POWER SUPPLY D SL 1750737 SL 6750507 CIRCULATING FAN MOTOR SL 4880507 27
3. WATERJACKET 2 INCUBATORS WITH MICROPROCESSOR CONTROL SCOSW SCOSW 2 SCO10W SCO10W 2 Previously designated 3517 3517 2 3524 3524 2 INSTALLATION AND OPERATION MANUAL 12 2013 4861607 Sheldon Manufacturing Inc P O Box 627 Cornelius Oregon 97113 EMAIL tech Shellab com INTERNET http www Shellab com Shellab 1 800 322 4897 503 640 3000 FAX 503 640 1366 TABLE OF CONTENTS SECTION 1 0 SECTION 2 0 SECTION 3 0 SECTION 4 0 SECTION 5 0 SECTION 6 0 SECTION 7 0 SECTION 8 0 SECTION 9 0 SECTION 10 0 SECTION 11 0 These units are CO2 Water Jacketed incubators for professional industrial or educational use where the preparation or testing of materials is done at approximately atmospheric pressure and no flammable volatile or combustible materials are being heated These RECEIVING AND INSPECTION GRAPHIC SYMBOLS INSTALLATION CONTROL PANEL OVERVIEW OPERATION DATA LOGGER FUNCTION FYRITE CO2 CHECKING HEPA C02 FILTER MAINTENANCE TROUBLESHOOTING PARTS LIST UNIT SPECIFICATIONS SCHEMATICS units are not intended for hazardous or household locations or use INTRODUCTION Thank you for choosing a water jacket incubator These units are not intended for use at hazardous or household locations Before you use the unit read this entire manual carefully to understand how to install operate and maintain the unit in a safe manner Your satisfaction with the unit will be maximized as you read about its safety and op
4. amperage should be virtually at maximum rated data plate amperage 3 do all controller functions work 4 is the OTP set high enough for diagnostics should be fully clockwise with the pilot light never on 5 has the fuse circuit breaker blown 1 0 1 may be normal 2 is fan working remove top panel and verify movement of cooling fan in center of shadow box 3 is ambient room temperature radically changing either door opening or room airflow from heaters or air conditioning stabilize ambient conditions 4 sensor miss located damaged or wires may be damaged check mounts for control and OTP sensors then trace wires between sensors and controls 5 calibration sensitivity call Customer Service 6 OTP set too low be sure that OTP set point is more than 5 degrees over desired Main set point check if OTP pilot is on continuously turn controller knob completely clockwise to see if problem solved then follow instructions in section 5 8 E for correct setting 7 electrical noise remove nearby sources of RFI including motors arcing relays or radio transmitters 8 bad connection on temperature sensor or faulty sensor check connectors for continuity and mechanical soundness while watching display for erratic behavior check sensor and wiring for mechanical damage 9 bad connections or faulty capacitor check connectors for mechanical soundness and look for corrosion around terminals or signs of arc
5. 1 7 2 7 7 4 7 5 7 6 7 7 7 8 Press button top of canister to release concentration Tip canister to the side to ensure all fluid is released from top of canister Loosen screw on slide scale and align top of fluid with zero on the scale Tighten screw Connect hose and aspirator bulb to unit being tested The sample port for connection is located on the control panel Place the hose sampling cap directly over the plunger valve on top of canister and depress firmly With button depressed squeeze bulb 27 times the last squeeze and with bulb still deflated release hose from button Turn Fyrite canister upside down 3 times each time allowing all fluid to flow to the opposite end of the canister Tip canister to the 45 degree position to ensure all fluid has been released from top of canister Read concentration in NOTE Your Fyrite indicator will come with a complete set of detailed instructions which should be followed carefully The fluid used inside this Fyrite instrument is poisonous and corrosive and must not be taken internally In the event of a spill or accidental body contact with the Fyrite fluid follow the instructions on the refill bottle carefully 16 HEPA 02 FILTER The CO2 Incubator design features a HEPA filtration system with a patented copper housing Patent Number 6 333 004 As the chamber air is drawn through the filter system airborne microbes and
6. be exercised any time access is made into areas housing electrical components Repair replacement or adjustment of components in these areas must only be done by qualified technicians familiar with electrical circuitry and the operation of the chamber Always make a visual inspection of the incubator and control console when troubleshooting Look for loose or disconnected wires or tubing which may be the source of the trouble NOTE IF BREAKERS TRIP REPEATEDLY OR FUSES BLOW REPEATEDLY CALL QUALIFIED SERVICE PERSONNEL TEMPERATURE Temperature too high 1 controller set too high see section 5 3 2 controller failed on call Customer Service 3 wiring error call Customer Service Display reads HI or 400 probe is unplugged is broken or wire to sensor is broken trace wire from display to probe move wire and watch display to see intermittent problems Chamber temp spikes over set point and then settles to set point recalibrate see section 5 4 Temperature too low 1 OTP too low see section 5 8 E 2 controller set too low see section 5 3 3 unit not recovered from door opening wait for display to stop changing 4 unit not recovered from power failure or being turned off incubators will need 24 hours to warm up and stabilize 5 element failure see if heating light is on compare current draw to data plate 6 controller failure confirm with front panel lights that controller is calling fo
7. electrician e Avoid damaging the power cord Do not bend it excessively step on it place heavy objects on it A damaged cord can easily become a shock or fire hazard Never use a power cord after it has become damaged e Do not position the equipment in such a manner as to make it difficult to disconnect power cord or coupler e Do not attempt to move the unit while in operation or before the unit has been allowed to cool RECEIVING AND INSPECTION IMPORTANT READ THIS INSTRUCTION MANUAL IMMEDIATELY Your satisfaction and safety require a complete understanding of this unit including its proper function and operational characteristics Be sure operators are given adequate training before attempting to put the unit in service NOTE This equipment must be used only for its intended application any alterations or modifications will void your warranty 1 1 1 2 1 3 1 4 Inspection The carrier when accepting shipment also accepts responsibility for safe delivery and is liable for loss or damage claims On delivery inspect for visible exterior damage note and describe on the freight bill any damage found and enter your claim on the form supplied by the carrier Inspect for concealed loss or damage on the unit itself both interior and exterior If any the carrier will arrange for official inspection to substantiate your claim Save the shipping crate until you are sure the unit has been delivered in good condition Return S
8. isolated particulates are not only removed from the air but destroyed as well It is through copper oxidation that the contaminants are actually killed The HEPA filter is easily replaced without the need of any tools and has an efficiency rating of 99 97 at 0 3 microns The Hepa filter attaches to a fixed coupling ring with a direct snap in action Push straight up to the coupling ring to connect Pull the filter straight down to disengage Replacement Hepa filters is available from Sheldon Mfg Inc Part Number 2800517 Install the HEPA Filter onto the duct prior to installing the duct in the unit WARNING Ensure that when installing the duct to ensure that the duct is not touching the C02 sensor head as this may cause erroneous readings 17 NOTE Prior to any maintenance or service on this unit disconnect the service cord from the power supply 9 1 Cleaning Cleaning and decontamination are recommended on a regular basis To prepare the incubator for cleaning remove all interior parts such as the shelf standards and false top All stainless steel parts are autoclavable Please review the Cleaning procedures described in the Installation section for detailed instructions 9 2 Check supply periodically don t let it run out The internal auto tank switch is not alarmed 9 3 supply lines and connections can be checked for leaks with a liquid soap solution Apply liquid soap solutio
9. loaded incubator being operated without shelving 10 CO2 attached to sample port instead of CO2 IN fitting 12 if display and Fyrite does not match see Troubleshooting Co2 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 debris in solenoid causing it to leak continuously solenoid failed while open unit miss wired controller output failed or shorted CO2 sensor or interface failure CO2 sensor plugged by debris or condensation restrictor partially plugged filter overly dirty or partially plugged tank regulator set too low see section 5 9 hose kinked or leaking poor door seal tank contains mixed gas not 100 medical grade CO2 CO2 tank empty solenoid failed while closed controller output failed while open restrictor plugged CO2 hose blockage 6 CO2 filter plugged 7 set point is at 0 0 and has not been reset see section 5 3 and 5 4 21 Is unstable display or actual reading varies around set point Can t adjust set points or calibration locked up Feeding continuously or abnormally high 2 usage Won t hold calibration Fyrite reading varies but display stable Low CO2 alarm activated and nGS NO Gas warning flashing on CO2 display 1 calibration error clear chamber for 12 hrs and confirm at 0 2 turn set point up and down to see if solenoid valve works by feeling and listening to valve 3 assure that IR tube block is completely t
10. minutes on the occurrence of a LOW alarm This time delay prevents the audible alarm from activating every time the door is opened and the temperature and CO2 drops Description of nGS NO Gas Warning Function If equipped The 2 Low alarm activates 15 minutes after the CO2 level drops more than 1 below the set point If the cause is not corrected in the next 5 minutes the CO2 display will flash nGS indicating a no gas warning nGS will continue to flash until the CO2 level is within 1 of set point or above 4 if the set point is 5 The audible alarm can be muted but the visual low alarm indicator will continue to be lit and the display will continue to flash nGS until the low alarm deactivates No action is required by the user other than to correct the cause of the CO2 drop Note It may take several hours after loss of gas before the CO2 low alarm comes on This depends on the unit s natural rate of decay if no CO2 is injected CO2 low alarm does not activate until CO2 has fallen more than 1 of set point or below 4 if set point is 5 Restore gas supply Check CO2 tank gas supply lines to the unit As soon as the gas supply is restored and the CO2 level rises to less than 1 below set point above 4 0 if set point is 5 the display will stop blinking nGS and will again show the sensor reading No action is required by the user other than correcting the cause of the drop in gas supply The audible alarm can be muted but the
11. out of balance condition Replace the fan or blower wheel if it is damaged or out of balance 3 Turn the motor shaft to make sure that it spins freely If it binds or the bearings make a rubbing or scrapping sound then replace the motor Solenoid valve buzzing After removing solenoid clean with alcohol for carbon build up then blow out Check valve seat or channels for contamination Check CO2 filter and or grade of CO2 used If seat is worn replace OTHER Controller on at all times locked up 1 turn unit off and on to reset 2 if cannot change any condition on the front panel call Customer Service Front panel displays are all off 1 Check for wire damage Unit or wall fuse circuit breaker is blown 1 check wall power source 2 compare current draw and compare to specs on data plate 3 see what other loads are on the wall circuit Unit will not turn on 1 check wall power source 2 check fuse circuit breaker on unit or in wall 3 see if unit is on e g fan or heater and just controller is off 4 check all wiring connections esp around the on off switch Unit is smoking Out of box Put unit under vent and run at full power for one hour Contamination in chamber 1 see cleaning procedure in Installation section 3 0 2 develop and follow Standard operating procedure for specific application include definition of cleaning technique and maintenance schedule NOTE THE TEMPERATURE OF THE GLASS DOOR AND THE INTERIOR CHA
12. same vapor pressure is maintained as long as any liquid is left in the tank When the last of the liquid has evaporated into gas then the pressure will drop rapidly as the gas is drawn off Only medical grade should be used in your incubator The use of any lesser grade may damage your incubator and void your warranty 13 5 10 5 11 5 12 5 13 The micro processor control system interprets the information from the CO2 sensor displays the CO2 concentration directly on the digital display reads the CO2 set point and controls the percentage of in the incubator chamber F The IR controlled unit is known as a direct sensor and operates under the principal that a certain frequency of infrared light is absorbed by CO2 The more is present the more light is absorbed Its accuracy is consistent no matter what the conditions are in the incubator The IR controller utilizes an infrared spectrophotometer to directly read the flow of CO2 gas into the chamber G It is recommended that the accuracy of your control system be monitored by measuring the actual concentration on a weekly basis with a Fyrite or other measuring device This should be done when the chamber has not been disturbed for an extended period of time after the weekend or first thing on Monday morning Setting the Control Attach the supply hose to the incubator and turn on the supply Set the control to t
13. to 20 PSI The internal tank switch will use up the 20 PSI first and then switch over to the 15 PSI tank It does not matter which tank is attached to which fitting When the 20 PSI tank is empty its output pressure gauge will indicate 15 PSI At this time the empty tank can be replaced Note that there are no alarms to indicate when the tanks are empty so operators should monitor gas consumption It is highly recommended that a good quality 0 60 PSI output range DUAL STAGE pressure regulator be used the CO2 tank The dual stage regulator will have two pressure gauges The high pressure gauge 0 4000 PSI will indicate the pressure within the tank The low pressure gauge 0 60 PSI will indicate the output pressure on the supply hose to the incubator Do not use single stage regulators They will not supply a stable pressure Just because a regulator has two gauges does not mean that it is a dual stage regulator Insure that you are using a dual stage regulator It is normal for the high pressure gauge on your regulator to start out reading 800 to 1000 PSI with a full tank The reading will drop to 500 to 800 PSI quickly and will stay there for most of the duration of the tank At the end of use the pressure will drop quickly to zero to indicate that the tank is completely empty Pure is in a liquid state in the tank and a constant vapor pressure is generated the tank above the liquid level The CO2 is drawn off of the top as a gas The
14. 46 1750846 Water Level Switch 7850575 7850575 Must specify 220V Jumpers on PCA must be reset from 115V to 220V 24 UNIT SPECIFICATIONS __ Weight Shipping All Models 560 Ibs 442 lbs Dimensions Exterior WxDxH IN Interior WxDxH IN Models 26 5 x 26 x 80 All Models each ne Temperature Range Uniformity 2 All Models Amb 8 C to 60 C 0 2 37 C 0 20 Model Amp Voltage DENZ n 5 110 120V 50 60 Hz a 3 5 amp 208 240V 50 60 Hz 5 110 120V 50 60 Hz oe Ha 3 5 amp 208 240V 50 60 Hz 25 FIGURE 6 WIRING DIAGRAM POWER INLET 6A SL 104179 GREEN CHERRY PRS ke eR et St ea ee ee ee os Se ie Sea ee ROUND POWER SWITCH SL 7850532 BLUE WATER FILL WATER FILL INDICATOR SWITCH SL 210004 SL 200020 CO2 PREHEATER SL 100909 6W USB BOARD SL 1750846 USB EXSTENSION 51 9 1800533 22207 59 PAOR H COMMUNICATION HEATING INDICATOR SL 200021 TEMP Cy 5 DISPLAY 4 SL 1750734 a e gt Freie A 10401 2 CII y MAIN TEMP ORANGE 4 3 sensor MAIN HEATING ELEMENT a gt TET Doon 4 LOOP CONTROL J5 SL 2350503 DISPLAY BOARD SL 1750725 n 2 w ware Fe
15. MBER WALLS MUST BE EQUAL TO PREVENT CONDENSATION The incubator is designed so that no internal electrical servicing should be required under normal conditions If electrical servicing is necessary it should be performed by qualified service personnel FOR PERSONAL SAFETY ALWAYS DISCONNECT THE POWER BEFORE SERVICING 23 PARTS LIST Description 115V 220V Anode 0260500 0260500 Cable Panel Mount USB Type B Extension 1800533 1800533 CO2 Display Board 1750735 1750735 CO2 Filter 2800525 2800525 CO2 Pre Heater 2350546 2350551 Cord Set 1800510 1800539 Cord Set European N A 1800500 Data Logger 1750537 1750737 Door Heater 2350550 2350550 Door Switch 7850578 7850578 Element Coil 2350503 120071 Fan Blade 2600526 2600526 Fan Motor 4880508 4880507 Fuse 3300516 3300515 Glass Door Latch 9530516 9530516 Gasket 8 feet 3450644 3450644 HEPA Filter 2800517 2800517 Humidity Display 1750736 1750736 Humidity Pan 995 00015 995 00015 Humidity Sensor 9990623 9990623 IR Transmitter 8320510 8320510 Leveling Feet 2700506 2700506 Overtemperature Safety 1750860 1750860 Pod Control 4 Loop 1750725 1750725 Power Inlet Fuse 3300516 3300515 Power Inlet 4200522 4200522 Power Switch 7850532 7850532 Shelf Assembly 9750581 9750581 SL Logo Slug 5800529 5800529 Solenoid Valve 8600528 8600529 Temperature Display Board 1750734 1750734 USB TO RS232 Bridge Board 17508
16. d fill INSTALLATION Local city county or other ordinances may govern the use of this equipment If you have any questions about local requirements please contact the appropriate local agency Installation may be performed by the end user Under normal circumstances this unit is intended for use indoors at room temperatures between 5 and 40 C at no greater than 80 Relative Humidity at 25 and with a supply voltage that does not vary by more than 10 The unit s technical and environmental specifications includes altitude not exceeding 2000 m operating and Pollution Degree of 2 Customer service should be contacted for operating conditions outside of these limits CAUTION Make sure that the incubator is located in its intended position and level before filling the water jacket Directions for filling the jacket are in Section 5 2 3 1 Power Source The unit power requirements are listed on the data plate PLUG THE UNIT INTO A PROPERLY GROUNDED AND RATED RECEPTACLE OF THE CORRECT STYLE A separate circuit is recommended for this unit to prevent loss of product due to overloading or circuit failures caused by other equipment The power supply must be properly grounded earthed and correctly sized to match the unit nameplate rating The supply voltage must match the nameplate voltage within 10 The US market units are designed for 120 volts 60 Hz 5 Amps The EU market units are designed for 230 volts 50 Hz 3 5 Amps If suppli
17. econd will cause the logger to step to the next parameter The table on the following page shows the indications for each parameter If no buttons are pressed for fifteen 15 seconds the panel display will revert to the normal operation mode THE CONTROL PANEL DISPLAYS SIX PARAMETERS SHOWN BELOW WHICH CAN BE CHANGED USING THE CONTROL PANEL BUTTONS LEFT TWO DIGITS RIGHT TWO DIGITS NORMAL OPERATION SHOW HOURS OF CURRENT 24 SHOW MINUTES OF CURRENT BLINK TIME TIME SET LOGGING PERIOD SHOW LP BLINK LOGGING INTERVAL IN OFF MINUTES CAN BE VERIFIED FROM 0 99 MINUTES SET HOURS BLINKS HOURS PORTION OF ARE BLANK ON CURRENT TIME CAN BE VARIED FROM 00 TO 23 SET MINUTES ARE BLANK BLINK MINUTES PORTION OF ON CURRENT TIME CAN BE VARIED FROM 0 TO 59 SET MONTH BLINK CURRENT MONTH CAN BE ARE BLANK OFF VARIED FORM 1 TO 12 SET DATE ARE BLANK BLINK DAY OF MONTH CAN BE OFF VARIED FROM 1 TO 31 CURRENT YEAR 19 OR 20 BLINK LOWER 2 DIGITS OF YEAR 6 3 The data logger clock has a back up battery The recommended replacement is Panasonic 3 Volt 165 BR2325 type 1HB 1HC OR 1HG 15 CHECKING Bacharach FYRITE 2 Gas Analyzer is recommended to measure CO2 concentrations the incubator chamber This test instrument is not supplied with the incubator but is readily available from your dealer Follow the instructions provided with each Fyrite instrument carefully to insure correct and accurate readings 7
18. ed with a detachable cord set plug the female end into the inlet on the unit and the male plug into the supply Assure that units requiring fuses have them installed The fuse is a type 10 amp for 120 vol units 6 3 amp for 230 volt units This fuse may be at the inlet or a part of the cord set male plug The unit must be located to easily allow for it to be unplugged or disconnected 3 2 Location When selecting a site for the unit consider conditions which may affect performance such as heat from steam radiators ovens autoclaves etc Avoid direct sun fast moving air currents heating cooling ducts and high traffic areas To ensure air circulation around the unit allow a minimum of 10cm of clearance between the incubator and surrounding walls or obstructions to free air flow 3 3 Lifting Handling These units are heavy and care should be taken to use appropriate lifting devices that are sufficiently rated for these loads Units should only be lifted from their bottom surfaces Doors handles and knobs are not adequate for lifting or stabilization The unit should be completely restrained from tipping during lifting or transport All moving parts such as shelves and trays should be removed and doors need to be positively locked in the closed position during transfer to prevent shifting and damage 3 4 Leveling The unit must sit level and solidly Leveling feet are supplied and must be installed in the four holes in the bottom corners o
19. erational features Keep this manual on hand so it can be used by all operators of the unit Be sure all operators of the unit are given appropriate training before you put the unit in service Note Use the unit only in the way described in this manual Failure to follow the guidelines and instructions in this manual may be dangerous and illegal General Safety Considerations Your incubator and its recommended accessories have been designed and tested to meet strict safety requirements For continued safe operation of your incubator always follow basic safety precautions including e Read this entire manual before using the incubator e sure you follow any city county or other ordinances in your area regarding the use of this unit e Use only approved accessories Do not modify system components Any alterations or modifications to your incubator may be dangerous and will void your warranty e Always plug the unit s power cord into a grounded electrical outlet that conforms to national and local electrical codes If the unit is not grounded parts such as knobs and controls may conduct electricity and cause serious injury e Do not connect the unit a power source of any other voltage or frequency beyond the range stated on the power rating on the dataplate inside the door of the unit e Do not modify the power cord provided with the unit If the plug does not fit an outlet have a proper outlet installed by a qualified
20. f the unit With the feet installed and the unit standing upright each foot can be raised by turning it in a counterclockwise direction Adjust the foot at each corner until the unit stands level and solid without rocking If the unit must be moved drain all of the water from the unit and turn the leveling feet in clockwise all the way to prevent damage while moving 3 5 Cleaning The unit chamber should be cleaned and disinfected prior to use The operating conditions and appropriate protocol will determine the correct procedure for decontamination A typical decontamination procedure that is adequate for many situations has been described below As well certain steps are listed that will help reduce the likelihood of contamination and the necessity of decontamination Whatever process is appropriate it needs to be done on a regularly scheduled basis Depending on usage and protocol this may be monthly quarterly or otherwise 6 Regardless of the decontamination procedure used certain precautions will need to be taken A Always disconnect the unit from the electrical service when cleaning Assure all volatile or flammable cleaners are evaporated and dry before reconnecting the unit to the power supply Special care should be taken when cleaning around sensing heads to prevent damage If you use chlorine based bleaches or abrasive cleaners this will modify the stainless steel interior finish DO NOT USE hard tools such as metal wire bru
21. he desired set point using the procedure described in section 5 3 Adjusting Display After the incubator has had several hours to stabilize at CO2 set point measure the actual CO2 with If there is any difference between the Fryite and the display use the procedure described in section 5 4 for making the display matching the Fyrite See Section 7 0 for Fyrite use NOTE When using the Fyrite insure that gas is not being injected while the reading is being taken Change the CO set point to 0 0 prior to taking the sample and then change the set point back to the desired value after the use of the Fyrite is finished Audible Alarms The temperature control and the control are both equipped with visual and audible alarms A Temperature Alarms The temperature control has alarm indicators for high and low conditions that are activated whenever the actual temperature is 1 above or below the set point B Alarms The controller has alarm indicators for high and low alarm conditions that are activated whenever the actual CO2 is 1 above or below the set point Audible Alarms Both controls are equipped with audible alarms that are activated when either of the HIGH or LOW indicators are activated The audible alarms can be muted for a single alarm occurrence by pressing and holding down either the UP or DOWN button for several seconds until the alarm mutes There is a built in delay of approximately 15
22. hipment for any reason you must return the unit contact your customer service representative for authorization and supply nameplate data For information on where to contact Customer Service please see the manual cover Accessories Make sure that all of the equipment indicated on the packing slip is included with the unit Carefully check all packaging before discarding Each chamber comes equipped with 3 shelves 6 slides 1 humidity pan and a power cord GRAPHIC SYMBOLS Your incubator has been provided with a display of graphic symbols which is designed to help in identifying the use and function of the available user adjustable components 2 1 2 2 2 3 2 4 2 5 2 6 2 7 2 8 2 9 2 10 2 11 2 12 2 13 2 14 A 4 CO2 el RH N x Indicates that you should consult your manual for further description or discussion of a control or user item Indicates Temperature Indicates Over temperature safety Indicates Degrees Centigrade Indicates Carbon Dioxide Indicates Gas for this unit Indicates AC Power Indicates Water Jacket Low Indicates OFF O Indicates Relative Humidity Indicates Percent Relative Humidity Indicates Potential Shock Hazard behind this protective partition Indicates Protective Earth Ground Indicates Unit should be recycled Not disposed of in lan
23. i dish cover is a method that raises it off the shelf and prevents it from rolling and hiding the scale from view For ease of reading the thermometer would be better if they were electronic with a remote display so they could be read without opening the doors Initial Control Settings Set controls to the set point you want your incubator to operate at A Set the OTP to its maximum position clockwise After making initial control settings allow the incubator to warm up and stabilize for 24 hours B All units are shipped set for 37 and OFF If other conditions are desired follow the instructions in section 5 3 for resetting the controls Temperature Calibration It is important that the calibration be performed in the following order Main temperature and then the Over Temperature Safety A During main temperature calibration it is important that the inner glass door not be opened for any reason and that the outer heated door is opened as little as possible B After the incubator has been running for 24 hours and the temperature display is stable at the set point read the temperature in the chamber from the reference thermometer and if necessary recalibrate the display using the instructions in section 5 4 After the main temperature display has restabilized and maintained set point for several hours check the actual temperature again If it does not match the display repeat the calibration D After the Main Temperatu
24. ight 24 series 4 if display is varying when doing Fyrite test have a leak on IR base or mounting plate or 2 is injecting at time of test 5 take voltage reading across sensor leads 0 0V 20 1V 6 assure that sensor is properly mounted 7 DISPLAY LOWER THAN FYRITE air leak around the CO2 sensor or the fan motor shaft 8 DISPLAY HIGHER THAN FYRITE leak in tube from sample port to chamber verify with liquid soap solution 9 DISPLAY HIGHER THAN FYRITE Fyrite fluid worn out replace fluid with Fyrite refill 10 DISPLAY HIGHER THAN FYRITE poor Fyrite sampling technique see section 6 0 11 DISPLAY HIGHER THAN FYRITE defective Fyrite pump or tubing 12 2 sensor interface or controller failure 1 confirm that fan is working 2 check for air leak around CO2 sensor or fan motor shaft 3 magnetic field interference 4 door not sealing properly 5 atmospheric pressure fluctuations 6 defective Fyrite pump or tubing 7 2 entering chamber too quickly a tank pressure too high b regulator set wrong c restrictor incorrect or missing 8 electronic problem with CO2 sensor interface or controller 9 top of unit exposed to cold air drafts 10 unit being operated without shadow box cover 11 electrical interference atmospheric or conducted 12 incubator too heavily loaded 1 turn unit off and on to reset processor in controller 2 if repeatedly happens call Customer Service 1 do decay test if more than 1 deca
25. ind the GAS IN fitting and in line with the tubing kit Units with flow meters require operator supplied air pumps These pumps should be checked for proper air filtering OPERATION FOR MINIMIZING CONTAMINATION 1 Keep the outside of the incubator including the air in the laboratory as clean as possible This is particularly important for units placed directly on the floor Do not place incubators near doors air vents or other areas of high air movement or traffic The floor around the unit needs to be clean Units that are placed on the floor should be mounted higher typically on a caster platform for ease of moving the unit during cleaning and access to the back of the unit Minimize the number of times access is made to the chamber during normal operation Do not depend on the use of antibiotics to maintain uncontaminated conditions as this is an inadequate technique for sterilization Preferably use aseptic techniques as described above for maintaining sterile conditions in the incubator 3 6 Shelves and Interior Parts Shelving shelf supports and a humidity are supplied with each unit See Figure 1 for proper placement of these accessories Figure 1 Align shelf slider between shelf stondord and slide into place at a slite angle in shelf standard slots Then lift ond rotating into ploce until base of slide is parallel to liner botton As illustrated fron the botton up Install shelf ftando
26. ing or other visible deterioration 1 assure that set point is at least 5 degrees over ambient room temperature 2 see if ambient is fluctuating 1 calibration error see section 5 4 2 temperature sensor failure evaluate if pilot light is operating correctly 3 controller failure evaluate if pilot light is operating correctly 4 allow at least two hours to stabilize 5 verify that reference thermometer is certified 1 turn entire unit off and on to reset 2 if repeatedly happens call Customer Service This can be a normal condition when operating temperature varies widely For maximum accuracy calibration should be done at or as close to the set point temperature 20 CO2 LEVEL Overshoots set point but stabilizes display and Fyrite match Overshoots set point and continues to rise display and Fyrite match Rises very slowly Never rises Display and Fyrite reading do not match 1 turn set point up and down to see if solenoid valve works by feeling and listening to valve 2 3 4 5 6 7 8 9 recalibrate with Fyrite see section 5 11 and section 7 0 fan not operating correctly a fan motor stopped b fan blade fell off c wrong fan blade installed or mounted backwards wrong restrictor installed or missing altogether tank pressure too high see section 5 9 A CO2 sensor partially plugged with dirt or condensation regulator set wrong see section 5 9 incubator too heavily
27. ition in the incubator chamber If no buttons are pressed within five 5 seconds the blinking will stop and the display will revert to showing the process or actual parameter within the incubator chamber Humidification Humidification of the unit is achieved by evaporation of water from the chamber humidity pan placed in the bottom of the incubator By filling this stainless steel reservoir pan with DISTILLED WATER ONLY and allowing this water supply to heat and evaporate near saturation 11 5 6 5 7 5 8 humidity is obtained Do not use plastic glass or other metals Only 300 series stainless metals acceptable for this reservoir pan Do not use corrosive chemicals including copper sulphate or chlorine in the pan or chamber as damage may occur Use Distilled Water Only DO NOT USE DEIONIZED WATER Use of disinfecting chemicals in the pan can change the surface tension of the reservoir water thus preventing evaporation and proper humidification of the chamber Water in the pan should be changed and the pan cleaned at least once a week to help control contamination and to maintain proper surface tension Temperature Monitoring To insure that the incubator is operating at the desired temperature an accurate temperature indicator such as a certified reference thermometer should be placed in the incubator chamber Try to place the thermometer in the center of the chamber and spaced off the shelf taping the thermometer down to a petr
28. le Port This is located on the front left side of control panel A sample can be drawn to measure the content the chamber at this port 10 READ THIS SECTION IN ITS ENTIRETY BEFORE ATTEMPTING OPERATION IMPORTANT In order to ensure the proper operation of this incubator it is essential that you regularly verify the accuracy of the temperature and controls Temperature can be confirmed by use of a thermometer inside of the chamber can be confirmed with the use of a Fyrite Gas Analyzer or other equipment such as a gas chromatograph These must be checked at least on a weekly basis Any questions should be directed to a technical service representative 5 1 5 2 5 3 5 4 5 5 Power up the unit by attaching the cordset supplied with the accessories to the unit inlet and the building power supply Verify that the power fuses are installed Attach the female end of the cordset into the fused and recessed male plug on the rear of the unit and the male plug end of the cordset into the building supply receptacle Turn the power switch to the I ON position Filling and Draining The front panel of the incubator has a WATER LOW warning light Fill the water jacket with water by means of the fill port located at the top right rear of the unit marked FILL WJ HERE Stop filling when the WATER LOW warning light goes off When full the unit holds approximately 19 U S gallons 72 liters To drain the uni
29. n to all areas of the supply line and observe for bubbles that will indicate a leak 9 4 Keep the flow system free of impurities Erratic CO2 control is usually traceable to the CO2 pressure regulator on the tank impurities in the tank or impurities in the solenoid valve Replace the CO2 in line filters every six 6 months or when the filters have become noticeably dirty on the upstream side There is a CO2 filter attached to the tubing kit and one inside the shadow box connected to the GAS IN line 9 5 If the data logger fails to function as specified check to verify that the port connections are solid and not loose If the data logger still fails to function properly call Customer Service for assistance 9 6 There is no maintenance required on any controls If the controls fail to operate as specified please review Section 9 0 Troubleshooting prior to contacting customer service 9 7 Storage If the incubator is to be turned off for any length of time dry the chamber and humidity pan thoroughly and leave at room temperature Failure to do this may cause the interior to become contaminated No adjustment to controls should be required when restarting the unit If the unit is to be shut down for transport drain the water jacket remove humidity pan and shelves dry the chamber and disconnect the CO2 and power supply s See section 3 3 Lifting Handling for transport procedures TROUBLESHOOTING 4 _CAUTION Extreme caution must
30. n value can be reset by setting the CO2 levels down until CL shows on the display Then resetting the CO2 control to the desired setting resets the 9 4 7 4 8 4 9 4 10 4 11 4 12 injection rate and limit values to that which was input by the operator Injection Light This pilot lamp is on whenever the controller is injecting into the chamber Humidity Display This digital display is marked RH and indicates the relative humidity in the chamber Datalog Communications Interface This controller provides six 6 parameters to externally log data of all the front panel displays The MODE button is used to select the parameter and the UP DOWN buttons are used to change the parameter s value Over Temperature Safety Control OTP Located front left of the control panel this is a hydraulic thermostat that is wired between the Main Temperature Controller and the heating element and functions as an override control If at any time the Main Temperature Control fails in the ON position and the temperature in the chamber rises above its set point the OTP is activated and maintains temperature at its own set point Note that the HEATING indicator will continue to function under the control of the OTP Interface Ports Located on the front left of the control panel opposite the Over Temperature Safety these three 3 ports are where the logged data can be externally accessed through serial and parallel functions Samp
31. r heat 7 OTP failure confirm with front panel lights that it is operating correctly 8 wiring problem check all functions and compare wiring to diagram in section 10 0 especially around any areas recently worked on 9 loose connection check shadow box for loose connections Display reads LO 1 sensor is plugged in backwards reverse sensor wires to controller 2 if ambient room temperature is lower than range of unit compare set points and ambient temperature to rated specifications in section 9 0 Unit will not heat over a temperature that is below set point 1 confirm that fan is moving and that amperage and voltage match data plate check fan motor motion in shadow box and feel for air movement in chamber 2 confirm that set point is set high enough turn OTP counterclockwise Unit will not heat up at all Indicated chamber temperature unstable Will not maintain set point Display and reference thermometer don t match Can t adjust set points or calibration Calibrated at one temperature but not at another and see if heating light or safety light comes on 3 check connections to sensor 4 check calibration using independent certified reference thermometer follow instructions in sections 5 4 and 5 8 1 verify that controller is asking for heat by looking for controller light if pilot light is not on continuously during initial start up there is a problem with the controller 2 check amperage
32. rd by aligning the large end of keyhole 5 both ends of standard with grove in pen and slide down 3 7 Download USB Software Go to www shellab com website home page and scroll down towards the bottom of the page Click on this section To use your SHEL LAB CO2 Incubator USB features you need to download the software by clicking on the image below Open the Excel Spreadsheet 5010092 and download information CONTROL PANEL OVERVIEW All controls are located on the front panel Units with a detachable cord have a fused inlet located at the top rear of the control panel This inlet has a recessed male plug fuse and an EMI filtering system designed to filter out electrical interference This inlet also prevents any internally generated interference from feeding out to the power grid 4 1 4 2 4 3 4 4 4 5 4 6 Power Switch The I O ON OFF switch controls all of the power for the incubator and must be in the position before any systems are operational Both temperature and displays will illuminate when the power switch is in the ON position Water Low Light This pilot lamp is on whenever the internal water jacket float switch has been tripped to the closed position When the water drops low enough the float switch closes the circuit turning on the indicator light O
33. res are set and adjusted the Overtemperature Safety Control needs to be set Do this by turning the OTP Control Knob counterclockwise until the Overtemperature Light comes on this will blink in sequence with Heating Activated Light Next slowly turn the knob clockwise until the light goes off then turn the knob another 8 degrees approximately This should set the Overtemperature 1 degree above the Main Temperature set point 12 5 9 Supply System and Control System The system is rated for pressures between 15 to 20 PSI which should never be exceeded at any time Prior to any attempt to hook up run the CO2 control system the water jacket must be filled and the temperatures adjusted as noted in section 5 7 Figure 3 PORTS PRE HEATER FILL RESTRICTUR SHUTTLE 2 VALVE GAS PORT HEPA FILTER SOLENOID VALVE PRESSUR REGULA SAMPLE 9851000 PORT The inlet fittings are located at the back of the unit near the top see figure three They are marked CO2 TO CHAMBER You have the option of using one or two tanks A supply hose with in line filter supplied with your accessories connects from each fitting to a tank and regulator If you plan to use only one tank it does not matter which fitting you connect the supply hose to and the remaining fitting is left open If you plan to use two tanks set the output pressure of one tank to 15 PSI and the other tank
34. shes or steel wool Use non abrasive cleaners and soft tools such as plastic brushes In the event hazardous material is spilled onto or into the equipment appropriate decontamination must be carried out If there is any doubt about the compatibility of decontamination or cleaning agents with parts of the equipment or with material contained please contact the manufacturer or his agent No decontamination or cleaning agents should be used which could cause a hazard as a result of a reaction with parts of the equipment or with the material contained in it TYPICAL DECONTAMINATION PROCEDURE 1 Remove the humidity pan every week and autoclave or wash with soap and water then disinfect with 70 alcohol solution Replace in the incubator with fresh DISTILLED water Flush the sample port tubing with 70 alcohol solution Replace any lines that have a green color Remove the door gaskets clean and disinfect Clean and disinfect all mounting grooves for the door gaskets Remove all shelves shelf supports shelf standards and shields Autoclave or wash and disinfect as described in item 1 Wash and disinfect all interior surfaces Give special attention to cleaning and disinfecting all access ports air bleeds shaft holes electrical pass through and any other passages into the chamber Replace all air and 2 filters every six months or when noticeably dirty on the upstream side COs filters are located the shadow box just beh
35. t hook a suitable hose to the siphon tube marked SIPHON HERE located to the left of the fill port Start a siphon and allow unit to empty into a suitable service drain or catchment area WATER RECOMMENDATIONS FOR USE IN WATER JACKET INCUBATORS The addition of a dis similar metal anode in Water Jacket incubators will allow the use of good quality tap water to fill the Jacket The anode is located in a threaded port near the fill port on the rear of the unit The anode will dissolve as it is in contact with mineral salts or dissolved gasses in the tap water The anode should be removed and checked for erosion every year When the anode appears to be over 50 eroded a new one should be purchased form Sheldon Mfg Inc Replacement Part 0260500 Tap water with a hardness of more than 30 parts per million PPM or 1 5 grains per gallon GPG will require assessment of the anode on a yearly basis Dissolved gasses should be no more than 120 PPM or 7 GPG The Ph of the water should test to between 6 0 and 8 5 When in doubt it is advisable to use DISTILLED WATER ONLY Setting the Controls Refer to the Control Panel Overview Section 4 6 of this manual Calibrating the Controls Push and hold both the UP and DOWN buttons simultaneously until the control displays CP for temperature or CO for CO2 after the display will start to blink While the display is blinking the UP and DOWN buttons can be used to adjust the display to match the actual cond
36. ver Temperature Light This pilot lamp is on whenever the Over Temperature Safety thermostat has been activated and taken control of the element During normal operating conditions this indicator should never be on Main Temperature Control This controller is marked and indicates the actual temperature within the chamber to 1 C The UP DOWN buttons are used for imputing the set point calibrating the display and muting or unmuting the audible alarm The HIGH and LOW alarm indicators will light whenever there is an alarm condition associated with the temperature within the chamber The MUTE indicator will light whenever the audible alarm has been deactivated Some alarm conditions have a delay programmed in Heating Light This pilot lamp is on whenever the Temperature Controller is activating the heating element C02 Control On Water Jacket Incubators The control sets the rate and the limit of injection of CO2 into the incubator based on input value and some variables The input value is set by the operator This is set at 5 CO2 in production At start up the incubator must be warmed up to operating temperature This is set in production at 37 C During warm up the water tray for humidity should be filled and placed inside This allows the humidity to reach 90 95 before 2 is injected The CO2 should be turned OFF during warm up Failure to do so will cause the control overshoot the set value input by the operator the injectio
37. visual low alarm indicator will continue to be lit and the display will continue to flash nGS until the low alarm deactivates 14 DATA LOGGER FUNCTION The data logger provides ways to externally indicate or log all the incubator front panel displays at intervals ranging from 1 to 99 minutes The logger contains a real time clock calendar to date and time stamp each logged reading The logger indicates and logs all front panel display readings in single lines of text This data is logged when the present logging interval has expired or one of the monitored displays has changed its alarm state Logged data has a time and date field that corresponds with the logger s real time clock This logged data can be externally accessed through ports provided on the front of the incubator for serial and parallel functions as well as a BNC contact closure plug for extended alarm condition s The parallel port is compatible with any Epson or IBM style printer The serial port operates at 9600 baud with 8 data bits 1 one stop bit and no parity and connects with a computer through the USB 6 1 Program the Data Logger Select the parameter to adjust by pressing and holding the MODE button pad until the left two display digits show LP indicating the logger has entered the set mode The parameter that can be changed will blink and its value can be changed by pushing the UP or DOWN arrow pads 6 2 Pressing and holding the MODE pad for approximately one s
38. y in an hour check for leak door gasket tightness motor inlet to chamber sensor or probe inlet to chamber chamber access tube plugged 2 check output signal from controller 3 check solenoid valve for correct operation 4 leak in plumbing including between regulator and CO2 tank 5 door being opened too often 1 atmospheric pressure fluctuations 2 top of unit exposed to cold air drafts 3 unit being operated without shadow box cover in place 4 condensation collecting on CO2 sensor 5 2 sensor or interface failure 6 unit incorrectly calibrated see section 5 4 7 taking Fyrite reading too soon after the door has been opened 8 air leak around CO2 sensor mounting plate 22 CO2 gas supply has been interrupted out Tank ran out of gas or did not switch over Pinched gas line tubing Severe leak in gas line Tubing is disconnected from the gas supply MECHANICAL Door not sealing 1 check physical condition of gasket 2 confirm that door latch pulls door in tightly 3 assure that gasket is in original location Motor doesn t move 1 if shaft spins freely check connections to motor and check voltage to motor 2 if shaft rubs or is frozen relieve binding and retest Motor makes noise 1 Make sure that the fan or blower wheel is not contacting its housing Adjust the motor mounting bracket position to re center the fan or blower wheel if necessary 2 Check the fan or blower wheel for damage or
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