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1. sSuntma Quick Ray Master UATM 272 c 8 Stat d Q im wt eS g Light Light Zoom Zoom Grab Live Open Save E2 34 5 Peo EH Initialize Cal Measure Run hiv B Tl Auto Manual Repor Setting Exit Image Position Pixel X 610 Pixel 191 Real Position mm x 25 328 mm 4 289 2008 02 29 14 01 58 How to use HELP function 1 Click the HELP button HELP 2 Click the button for which you would like to view help 3 Description message will be displayed about the specific button you have chosen 4 Click the HELP button again to close the message 9 UNITMA Quick Ray Master UATM 272 Operating Manual 5 11 Demo The demo mode is to check all components condition of the instrument and to see that the system is running normally DEMO Touch Demo button on the Main menu to show the demo screen Sgunitmma Quick Ray Master UATM 272 Occ mr Ql LIGHT ZOOM CAMERA FILE DONOR BLOCK CHANGE COUNT RECIPIENT BLOCK CHANGE COUNT RECIPIENT BLOCK 1mm 7 8 9 101112 Tir B eseoe a a a a a a E A O E E E E E EEEE E E E EE EEEE E E E EE EEEE E E E E EEEE 0 E E E EEEE EE E E E EEEE EE 12a peiie aiina s s s s s s s s 2 Cee Hl al a ale ee a a a tettette tettet tettette v gt gt B L CALIBRATION MEASURE DE BLOCK PR
2. Bottom right side part is for motion control Speed select is to apply the speed set at the Set Mode 5 6 Position shows the position of every axis where motor is located Push controls puncher needle inside the tip Rotate controls angular move of tip Red arrows controls X Y axis of movement Blue arrows control movement of cameras in Z axis Left arrow controls Cam1 and right arrow controls Cam2 Stop button instantly stops the motion control ue 99 UNITMA Quick Ray Master UATM 272 Operating Manual 5 6 SET MODE for calibration SET Mode will let you modify reference parameters in quick ray system SET Mode should only be performed by person who fully understands the system General users are not recommended to enter the setup mode or modify preset parameters e Modified parameters can result severe damage to the unit and possibly destroy mechanism Please consult your sales engineer before entering into Set mode SET mode can only be entered after type in ID and Password The password can be entered with the touch keyboard displayed on the screen ee 20o UNITMA Lowey meg See SUTRA jarkeiaea A DOBRO DEOEEDEGH fz xic vieinim Quick Ray Be so Quick Ray After entering correct password click on OK and below SET screen will appear TRET Quick Ray XI 5 6 1 Donor Block Parameter Click on Donor block Param button and below screen will appear Donor block posit
3. i 2y Ju a a Bw OWN Borkh als J l eee A eT Micmsot Otice Excel Bah FA iala s 4 Click the Save button __28 _ How to print the report 9 UNITMA Quick Ray Master UATM 272 Operating Manual User can print the report with an external printer connected to the instrument 1 Connect the printer to the USB port of the instrument Before printing be sure to install a printer driver according to your printer manufacture s installation manual To achieve this you will need to exit the program and go to Windows start icon from your desktop screen On Auto Mode screen touch Report button and a pop up menu will be displayed Press Print button and the program will display an Excel spreadsheet Make sure Office Excel Program is functional on the instrument PC 2 Click the Print button in the Microsoft Office Excel program i SEE o gt ie Hama age rT BH wono Bau Faa B Eee ain o T ae 2a gese Baa a PERRIN I O Re ee ee eee eee oO TMA Block Infomation DATE organization Position Tested By Sample Type Core Size amp Cour 99 UNITMA Quick Ray Master UATM 272 Operating Manual 5 9 Alarm sound Alarm sound gives notice that the instrument has finished the TMA block making process 5 10 HELP Click the HELP button for detail descriptions HELP is Key specific
4. 2 Pen marked position on donor block is located With proper image processing puncher tip located to the spot and acquire sample Below picture shows the tip punching the donor block 3 While the puncher holding the sample inside the tip it moves to the recipient block and inserts the sample into the hone on the recipient block Below picture shows the puncher inserting sample into the hole 4 After sample is properly inserted into the hole Quick Ray Master creates a sheet with recipient looking shell and writes accrued DONOR ID in the corresponding cell on the spread sheet This cycle of process from step 2 to 4 repeats in number of sample quantity X punch count 5 4 2 Block change When donor block is all sampled or recipient block is fully filled Quick Ray will display a message box for block change The stage will move to the front as if Set Tray button is pressed Replace donor block or recipient block as needed and press Complete button on the windows gt UNITMA Quick Ray Master UATM 272 Operating Manual message box Press Continue button to resume the process 5 4 3 Finish After processing all required number of samples Sample count Punch count Alarm message box will appear Below screen is what recipient block looks after Job finishes When the job finishes the spread sheet will be filled with the donor ID as shown below Tissue Array Research Program Come tlm 3 Tample 200
5. 99 UNITMA Quick Ray Master UATM 272 Operating Manual 5 General procedure of Tissue MicroArray 5 1 Building TMA with Quick Ray Master 5 1 1 Place the reference slide and the donor block on microscope stage for marking the position with an oil pen 5 1 2 Punch and deliver the tissue specimen from donor blocks to recipient blocks by using Quick Ray Master 1 Place donor blocks and a recipient block on the block platform in Quick Ray Master 2 Punching and delivering the tissue specimen from donor blocks to recipient blocks by using Quick Ray Master 3 Disassemble the recipient block from the Quick Ray master 5 1 3 Put the recipient block into embedding mold with cutting section faced down and incubate it in Oven at 60 C for 30minutes 26 S3 UNITMA Quick Ray Master UATM 272 Operating Manual 5 1 4 Embedding Process 1 Once the recipient block is completely transparent 2 Place the plastic cassette on top of this recipient block 3 Pour hot embedding paraffin 1 2 3 5 1 5 Solidify the block in cold plate 5 1 6 Cut the block with the microtome Anau 4um seessoesss Teer ee eeee sueecesers a 99 UNITMA Quick Ray Master UATM 272 Operating Manual 5 2 Preparation for punching and inserting with Quick Ray Master e Keep the cover door of the instrument a safe distance from your head and face e Be careful for your fingers or hand not to be scratch
6. Tasted Ey Typa n REPORT B a k Ari Manm Repor Seating image Posion Pie x Pinel Vda Read Pestionimm 2t 04 184 mm Yo foe AR eS How to use REPORT MODE 1 Click the REPORT MODE button The preview about the report appears Image Positions Pind 2280 Pinel W Meal Postensimm 32 2183 mm Yao MBSE 25 1617 6 el Prin 2 Click the Print button E Excel spreadsheet will be displayed z UNITMA Quick Ray Master UATM 272 Operating Manual i E 7 Booki Microsoft Excel _ ox 22 HOX A092 4 WR B 87 Gu AX A13 fe 1 G K ra TMA Block Infomation 3 4 5 DATE 2008 02 29 6 organization 7 Position 8 Tested By 9 Sample Type 10 Core Size amp Cour 11 12 5 B Mar Sheet Sheet Sheets FI 4 3 Input the information about the recipient block or TMA block 4 Save or print the report How to save the report User can save the report to the instrument s internal memory or the external USB memory 1 Click the Save button lea E Mii rosoft Excel Booki E HZ BIW ME RIEA TMA Block Infomation 2 Connect an external USB memory chip if you choose to save the report file into an external memory 3 Choose the destination disk or folder And enter the file name OF 0At AF AE AND 4 U BFE J G alax a Od a sain E La dala DI upaj LH Ke By BCD S201 tes WE A ey Adminiswator BA
7. User Manual Quick Ray Master Automatic Tissue Microarrayer UATM 272 ee rtm ma com Quick Ray Master UATM 272 Operating Manual NOTE This manual describes the installation and operation of Quick Ray Master UATM 272 Review this manual to avoid injury and prevent damage to this product or any products connected to it before you operate this instrument To avoid potential hazards use this product only as specified in this manual This documentation contains information and warnings that must be followed by the user to ensure safe operation and to maintain the product in a safe condition Customer shall be responsible for paying all shipping charges duties taxies and any other charges for any failure or damage or injury caused by improper use or improper or inadequate maintenance and care Unitma Co Ltd shall not be obligated to furnish service under this case a to repair damage resulting from attempts by personnel other than Unitma Co Ltd representatives to install repair or service the product b to repair damage resulting from improper use or connection to incompatible equipment c to repair any damage or malfunction caused by the use of non Unitma Co Ltd supplies or d to service a product that has been modified or integrated with other products when the effect of such modification or integration increases the time or difficulty of servicing the product Copyright Unitma Co Ltd All rights r
8. Loose the screw of back cover of the instrument with a screw driver 2 Open the top cover of the instrument 3 Check the terminal of the internal power supply in the instrument 99 UNITMA Quick Ray Master UATM 272 Operating Manual TERMINAL for changing 110V 220V LFG AC IN L AC IN N 4 To set the voltage of the instrument to 200 240V do not connect the terminal with the power wire 5 Fix the power wire to the terminal with a screw for 100 120V 4 4 Turning on off the instrument 4 4 1 Turning on the instrument 1 Connect the AC power cord to the instrument and then to the AC power source 2 Turn the Main AC power switch on 3 4 You will see the application software of the Quick Ray Master UATM 272 Push the PC power switch on 4 4 2 Turning off the instrument 1 Exit the application software of the Quick Ray Master UATM 272 by clicking the EXIT button 2 From the start menu on Windows desktop select turn off 3 AC power switch off Make sure that the PC monitor is off and all programs has been logged out before the main 99 UNITMA Quick Ray Master UATM 272 Operating Manual switch red color is turn off In case the PC doesn t turn off because of operating system software failure you can shut down the computer by pressing for 5 seconds the green button In this case make sure you have saved the data you were working on before turning off in this manner
9. 60Hz 90 kg 735 X 625 x 525 mm 10 C to 35 C Transportation Storage temperature range 5 C to 55 C Relative humidity Humidity during transportation storage Camera resolution Camera CCD Size 1 Pixel resolution Monitor embedded Tip s needle hole size Motor accuracy Time per 1 cycle Operating system Block capacity Recipient block Fuse specification Operating environment condition Over voltage Category Pollution degree Mains voltage fluctuation Max 80 non condensing lt 80 640 x 480 pixels 7um x 7um 3 45 um x 3 45 um LCD monitor 10 4 800x600 with a touch screen 1mm 2mm 3mm 5mm Repetitive Position Accuracy 0 02mm 10 15sec Pick amp Place Microsoft Windows XP 5 donor blocks 1 recipient block UNITMA recipient block consumable F3 15AL250V Indoor use only II Illa or Ib Up to 10 a UNITMA Quick Ray Master UATM 272 Operating Manual 3 4 Consumable Recipient block The patented recipient block is made of a special material that melts when heated at 60 C for 30 minutes The recipient block has pre made evenly spaced round orifices wells arranged in a square matrix array Four different recipient blocks are available 10 x 12 wells of 1mm in diameter 6 x 10 wells of 2mm in diameter 5 x 6 wells of 3mm in diameter 4 x 5 wells of 5mm in diameter Weights 3 5g Use only recipient blocks supplied by Unitma Co Ltd K UNITM
10. Cleaning the tip body and the tip e Keep the cover door of the instrument a safe distance from your head and face e Be careful for your fingers and hands not to be injured by the needle of tip or the block holder frame during assembling or disassembling or when cleaning the interior of the instrument 1 Unplug the AC power cord from the electrical outlet 2 Open the cover door 3 Disassemble the tip body from the tip body holder of the instrument i 99 UNITMA Quick Ray Master UATM 272 Operating Manual 4 Wipe the tip body and the tip gently with a damp cloth and then dry the surface Do not use solvents or other chemicals to clean the exterior of the instrument Cleaning the block holder frame 1 Unplug the AC power cord from the electrical outlet 2 Open the cover door 3 Disassemble the block holder frame from the instrument 4 Disassemble the donor block and the recipient block holder from the block holder frame 5 Wipe the recipient block holder top and the recipient block holder bottom and the block holder frame gently with a damp cloth and then dry the surface Do not use solvents or other chemicals to clean the exterior of the instrument 6 2 Replacing consumable Consumable list Four different size recipient blocks 10 x 12 wells of 1mm in diameter 6 x 10 wells of 2mm in diameter 5x6 wells of 3mm in diameter 4x5 wells of 5mm in diameter Replacing the recipient block A e Keep the
11. Purch 1 5 5 Manual Operation SS onrrma Quick Ray mp ee h QA00C010 06 Pem aoe a a w amp Lo x Arrow Key Recip Set Donor Set ue UNITMA Quick Ray Master UATM 272 Operating Manual While Auto operation program is running click on Manual button for manual operation If donor marking is not identified or recipient block hole is not found the operation will switch to Manual mode Donor Set lf donor marking identification failed this button becomes active The sampling area can be selected from the touch screen or use Arrow key located on the right side of Donor Set button to move To confirm the location click on the Donor Set button Gab number in the middle of arrow key indicates how many pixels to move when pressed Recip Set If recipient block hole is not clearly identified Recip Set button becomes active Using the same arrow key button the recipient block hole can be located and confirmed by Recip Set button Gab number in the middle of arrow key indicates how many pixels to move when pressed By pressing Move button under Recipient block the puncher moves to Recipient block the recipient hole position Donor block By pressing Move button under Donor block the puncher moves to the donor block position Motion Controller Speed select Gtep Low Middle High Load Push Position n ry FA ma a 2 Cam 2 Tip Rotate 4 Am TIP T Aot TPush O G Te y b
12. The values can be modified using NumPad and click on Save to store the value 5 6 5 Calibration Parameter Click on Calibration Param button and below screen will appear Distances between cameras 99 UNITMA Quick Ray Master UATM 272 Operating Manual and tip and each camera s resolution can be entered Each button is located in sequence of calibration and click on save button to store changes made SS uNITMA Quick Ray 1 Select Model Select tip size between 1 2 3 5mm Selected Tip will be positioned 2 Move Tip Pos Tip will move to the referenced position at Cal Tip Pos in Recipient Block Param Using the Motion controller on the screen locate the tip at the center of calibration mark 3 Register tip pos Save the reference value after Move tip pos is modified 4 Move CAM2 Calibration Pos Cam2 moves to the Cal Pos in the Recipient Block Param to the chosen tip size at Select Model While centering roundup to upper right side to prevent passing over FOV Siua Quick Ray Master UATM 272 Sl Aa a imm es L Cae oe 5 Calibrate CAM2 Camera s resolution in pixel is calculated The camera moves x and y position by both positive and negative 0 5mm and compare the mechanical movement with display and find the resolution and centers it This is to measure the distance between the Tip and Cam2 6 Register CAM2 Distance Distance between the tip and Cam2
13. be set 4 Pre marked Donor blocks should be inserted in the position on the STAGE according to S3 UNITMA Quick Ray Master UATM 272 Operating Manual sequence of MS Excel file Oil pen or White out marked donor block is inserted on the tray as shown above The marking on the donor block should be completely dried to avoid insertion error and ink buildups inside puncher When inserting recipient block into cassette and assemble pay attention to the direction of cassette blocks The slanted side should be oriented to the top left corner Otherwise the hole searching error will occur in operation j UNITMA Quick Ray Master UATM 272 Operating Manual Picture shows all blocks and Trays are set on the Stage 5 3 7 RIC Recipient block Insertion Check If selected Camera 2 checks for correct insertion after sample is released into recipient hole The failure is posts red dot instead of green and writes an error message in the spread sheet Loading a set Tray Ohh Omi m Select Recipoent Block lom oum When orms J Cnm pams 5 4 Auto run operation 5 4 1 Main operation Press Start button on the screen and Donor TMA procedure will start and proceed as programmed 1 Prior to the main activity the top left corner of recipient block is marked with 1mm puncher tip for orientation purpose ue 9 UNITMA Quick Ray Master UATM 272 Operating Manual
14. careful when moving the instrument to avoid any physical damage Q if lt lt L LY i UNITMA Quick Ray Master UATM 272 Operating Manual Floor loading requirement Ensure that the floor under the instrument is capable of supporting all other installed instrument The instrument is about 90kg Do not put any objects on the instrument or accessories Positioning the instrument 1 The left side of the instrument must remain unobstructed to ensure adequate airflow and prevent overheating inside the instrument Refer to next instruction Ventilation requirement 2 The interface port in the right side of the instrument is for connecting the cable for interfacing external devices Ensure that connecting cable is not obstructed Interface port Power cord 3 The power cord in the right side of the instrument is for connecting the power cable to AC power source Ensure that connecting cable is not obstructed 4 Allow the following clearances for normal system maintenance At the top of the instrument Atleast 2ft 60 96cm In the front of the instrument Atleast 4ft 121 92cm Ventilation requirement The air intake and exhaust areas must be free from obstructions Unrestricted air flow is required for proper cooling Ensure that air flow is not obstructed When installing or using the instrument provide at least 4 100mm clearance left from the instrument for proper cooling Re
15. cover door of the instrument in a safe distance from your head and face Be careful for your fingers or hand not to be injured by the needle of tip when assembling or disassembling the blocks from the frame holder Be careful not to compress your fingers when closing the cover door of the instrument 1 Wait until the operation of the instrument is finished and stopped 2 Open the cover door 3 Disassemble the block holder frame from the jig for block holder frame of the instrument 5 Disassemble the recipient block from the recipient block holder 4 Disassemble the recipient block holder from the block holder frame 6 Insert the new recipient block into the recipient block holder 7 Put the recipient block holder with the new recipient block into the block holder frame n UNITMA Quick Ray Master UATM 272 Operating Manual 8 Assemble the block holder frame into the jig for block holder frame of the instrument 9 Close the cover door 6 3 Replacing components Replaceable components list 1 Tip body 2 Tip 3 Block holder frame 4 Recipient block holder A e Keep the cover door of the instrument in a safe distance from your head and face e Be careful for your fingers or hand not to be injured by objects to be replaced Be careful not to compress your fingers when closing the cover door of the instrument Replacing the tip body 1 Unplug the AC power cord
16. distance from your head and face e Do not operate without covers Do not operate this product if covers or panels are removed e Keep the instrument and accessories out of the reach of children e Do not touch the plug of the AC power cord with wet hands e Be careful with your fingers or hands from being injured while assembling or disassembling the tip body or the block holder frame or when cleaning the interior of the instrument AC power cord use and Ground e Use Proper Power Cord Use only the power cord specified for this instrument and certified for the country of use Verify the voltage and frequency of AC power source e Ground the instrument This instrument is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground Before making connections to the input or output terminals of the product ensure that the product is properly grounded e When disconnecting the AC power cord hold it by the plug and pull straight out form the electrical socket Never pull by the cord or pull at an angle 23 UNITMA Quick Ray Master UATM 272 Operating Manual Use the instrument and accessories according to the instructions in this manual e Use the instrument and accessories according to the instructions in this manual Neither authorization for the analysis or modification of the instrument nor the analysis and use of its circuit confi
17. from the electrical outlet 2 Open the cover door 3 Disassemble the tip body from the tip body holder of the instrument 4 Assemble the new tip body into the tip body holder of the instrument O e Make sure the diameter of tip s needle of the new tip body is the same as with the diameter of tip s needle of the disassembled tip body Check that the position of the new tip body in the tip body holder is the same as with the position of the disassembled tip body 5 Close the cover door Replacing the tip 1 Unplug the AC power cord from the electrical outlet 2 Open the cover door 3 Disassemble the tip body from the tip body holder of the instrument 4 Loosen the screw of the tip and disassemble the tip from the tip body 5 Replace with new tip and tighten the screw then assemble the new tip into the tip body 6 Assemble the new tip body into the tip body holder of the instrument oe 99 UNITMA Quick Ray Master UATM 272 Operating Manual O e Make sure the diameter of tip s needle of the new tip body is the same as with the diameter of tip s needle of the disassembled tip body Check that the position of the new tip body in the tip body holder is the same as with the position of the disassembled tip body 7 Close the cover door Replacing block holder frame 1 Wait until the operation of the instrument is finished and stopped 2 Open the cover door 3 Disassemble the bloc
18. product have it inspected by Unitma Co Ltd e Avoid exposed circuitry Do not touch exposed connections and components with the power turned on e Do not operate in wet damp conditions e Do not operate in a high concentration of explosive gases atmosphere e Keep the instrument surfaces clean and dry e Do not allow liquid or small particles to get into the instrument or accessories e Do not throw or drop the system or accessories or subject the instrument to strong physical shock e Unplug the AC power cord from the electrical outlet before cleaning S3 UNITMA Quick Ray Master UATM 272 Operating Manual Using in proper environment e Provide proper ventilation Refer to the manual s installation instructions for details on installing the product so it has proper ventilation e Do not expose the system or accessories to high temperatures high humidity or direct sunlight e Do not expose the instrument or accessories to dust smoke or steam e Do not place the instrument on surfaces that are tilted unstable or subject to vibration e Do not put heavy objects on the instrument or accessories Do not use the instrument where oxygen gas is used To avoid personal injury e Be careful not to compress your fingers when closing the front door of the instrument e Use in a well illuminated area and keep the LCD screen in a safe distance from your face e Keep the cover door of the instrument in a safe
19. the certain height below to protect from operation damage Sampling is the height position where the tip is lowered and samples from the donor block Standby Thrust is the height where the tip inserts the sample into the recipient block It should be closely positioned to the recipient block ii UNITMA Quick Ray Master UATM 272 Operating Manual Recipient 2mm Selecting the recipient blocks at the combo box the value can be finely tuned for each tip s activity 5 6 4 Speed Parameter Click on Speed param Button for below screen ine motor control speed values can be modified 2S unrrma Quick Ray Cc Speed select an Load 7 ieii ae 7 al Bl te me al o is i Sens Rotate can 6 DD rir see for A amp BI 1 Low Low speed value can be edited This value does not have any influence in operation tack time The values can be modified using NumPad and click on Save to store the value 2 Middle Middle speed value can be edited This value does not have any influence in operation tack time The values can be modified using NumPad and click on Save to store the value 3 High High speed value can be edited This value does not have any influence in operation tack time The values can be modified using NumPad and click on Save to store the value 4 Auto All speeds values for Auto mode can be edited The value entered makes an effect in operation tack time
20. 8 Report 5 9 Alarm sound 5 10 Help 5 11 Demo 6 CLEANING amp MAINTENANCE 6 1 Cleaning the instrument S3 UNITMA Quick Ray Master UATM 272 Operating Manual 6 2 Replacing consumable 6 3 Replacing components 6 4 Maintenance 6 5 Repair 6 6 Disposal of waste 7 TROUBLE SHOOTING 7 1 Instrument failures 7 2 Operating errors with possible causes and corrective action 8 WARRANTY amp SERVICE S3 UNITMA Quick Ray Master UATM 272 Operating Manual 1 IMPORTANT NOTES 1 1 Explanation of Symbols used WARNING WARNING indicates an injury hazard not immediately accessible as you read this symbol It calls attention to an operating procedure practice or the like that if no correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met CAUTION CAUTION indicates a hazard not immediately accessible as you read this symbol It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met connected to grounded chassis Main Power Connected or ON C Main Power Not Connected or OFF eels Pushed PC POWER on Ll Pulled PC POWER off eS Protecti
21. A Quick Ray Master UATM 272 Operating Manual 4 SETUP 4 1 Installation site requirements Transporting the instrument A e Disconnect all external cables before transporting the instrument e Never attempt to transport the instrument by yourself Three or more people are required to transport the instrument for transporting without causing injury or damaging the instrument e Ensure that your footing is solid and balance the weight of the instrument between your feet e Transport the instrument slowly and never move suddenly or twist your body during transportation e Keep your back straight and transport with your legs not your back If you must bend down to transport the instrument bend at the knees not at the waist to reduce the strain on your back muscles The weight of the instrument is about 90 kg The instrument should not be moved frequently Before you install the instrument ensure that your site is properly prepared so you can avoid having to move the instrument later to accommodate power sources and network connections e To safely transport the instrument perform the following steps 1 Since the weight of the instrument is over 90 kg 200 lbs we recommend four adult people to safely move the instrument 2 Minimum of four people working together should hold each corner of the instrument 3 Each person holding one corner with both hands should move in coordination to move the instrument 4 Be
22. Hole lt is already registered with Hole Nothing to extract from a DONOR at this time Selected All Hole Do Initialize 62 Possible Cause and corrective action When running the Auto Mode neither the Donor block nor the Recipient block hole has been selected during the setting mode Please refer to section 5 8 Process Setting menu of this operating manual When during the Process Setting mode the hole of the Recipient Block has been selected two times this message will show Please select another hole on the Recipient block to receive the tissue specimen When using the Setting Mode the maximum number of punching holes from Donor block is 40 This error message will disappear automatically and no more selection will be accepted When the available number of holes in the Recipient block has been filled and no more tissue specimens from Donor blocks can be accepted To run the instrument initialization from Auto mode must be selected When this error message appears please initialize from the Auto mode menu screen 23 UNITMA Quick Ray Master UATM 272 Operating Manual Not Execute Excel To write the Report of the Recipient block you need Excel program installed and functional on the instrument s PC Please check for software installation instructions on your Window O S Not measured the height Block When Run button has been pressed without measuring the heights of each block this error
23. OCESS SETTING RUNNING MANUAL MODE REPORT MODE Image Position Pixel X 582 Pixel 169 Real Position mm 0 000 mm 0 000 2008 01 15 18 02 37 lt Demo Screen gt Demo To stop the demo mode click the oP button and click the exit button to return to main menu 55 S3 UNITMA Quick Ray Master UATM 272 Operating Manual 6 CLEANING amp MAINTENANCE 6 1 Cleaning the instrument e For safety unplug the AC power cord from the electrical outlet before cleaning e Do not allow liquid or small particles to get into the instrument or accessories when cleaning Cleaning the connectors Do not use when the connecting cables of the instrument and AC power cord are not clean Remove the dirt with a dry cloth or cotton swab When is used in unclean condition the flow of electrical current may be obstructed Cleaning the LCD screen Wipe gently with a soft cloth Be careful to avoid scratching the LCD screen Cleaning the exterior surface Wipe gently with a damp cloth and then dry the surface Do not use solvents or other chemicals to clean the exterior of the instrument Clean the ventilation grids at the side of housing panels with a brush or vacuum cleaner to remove dust and dirt Cleaning the interior surface Open the cover door and wipe gently with a damp cloth and then dry the surface Do not use solvents or other chemicals to clean the interior of the instrument
24. ace with an external monitor DVI Monitor nas 7r Mouse Be Keyboard USB e Interface with an USB memory stick USB Memory e Interface with a printer USB USB LAN ee f Printer Interface with an external PC LAN B PC Printer i UNITMA Quick Ray Master UATM 272 Operating Manual 4 3 Connecting to main power 4 3 1 Connecting to main power supply e A constant and stable AC power source supply to the instrument must be ensured at all times Failure to comply with the above will cause severe damage to the instrument e Always use a grounded power cord Do not use defective power cord 1 Set the AC power switch to the off position 2 Refer to Power cords The line cord provided is matched to the country of origin by UNITMA U K Europe U S A Switzerland Denmark 3 Connect the power cord 8m maximum to the rear of the instrument then to a suitable AC voltage source Ensure that you have the correct line cord See 3 3 Instrument specification of this manual 4 3 2 Selecting the voltage of AC power source A e Do not change the voltage of AC power without authorization from Unitma Co Ltd e Contact a service personnel in Unitma Co Ltd for changing the voltage of AC power source e Before selecting the voltage of AC power of the instrument verify if the voltage of AC power intended to use is same with voltage of AC power source 1
25. as indicated Level 2 practices plus special laboratory clothing controlled access Level 3 practices plus airlock system negative air pressure 11 None primary containment provided by adherence to standard laboratory practices during open bench operations Partial containment equipment i e Class or Il Biological Safety Cabinets used to conduct mechanical manipulative procedures that have high aerosol potential that may increase the risk of exposure to personnel Partial containment equipment used for all manipulations of infectious material Maximum containment equipment Maximum Containment 5 UNITMA Quick Ray Master UATM 272 Operating Manual 3 INSTRUMENT COMPONENTS AND SPECIFICATIONS 3 1 Instrument description e Tissue Microarray TMA Tissue MicroArrays are a collection of multiple tissue cores that are arranged in columns and rows inside a paraffin block allowing for histological analysis They are a crucial tool in the analysis of gene and protein expression levels in samples from normal and diseased specimens Further they are useful in the early stage discovery of gene targets in genomic research validating targets testing and optimization of diagnostic tests and in the quality control of molecular detection schemes And the Tissue array technology not only makes decrease the reagent time and human resource below one sixtieth but also can be applied most of the know h
26. bove UNITMA Quick Ray Master UATM 272 Operating Manual Locate the tip at the center 5 6 3 Tip Body Parameter Click on Tip Body Param to set the tip parameters of height and location SS UNITMA Quick Ray t Setting Mari ven Eh a il 1 Tip Rotate Ctrl Set tips distance values from the center Each tip is located at 90 degree and they are rotated and selected for use The values can be entered with NumPad and saved when Set button is clicked 2 Change Tip Pos Click on MOVE button tip will move to the Ready position defined in Recipient Block Param This is necessary for safe tip change Click on one of the tip size buttons 1 2 3 5mm to locate the corresponding tip to face the user In example click on 1mm button to locate 1mm tip face the user and this position is defined in 3mm in Tip Rotate Control 3 Z axis Donor Ctrl CAM1 s height position can be controlled in Z axis Donor Control Ready position and Donor Search Position can be set This value can be changed using NumPad and saved when Set button is clicked 4 Z axis Tip amp Recipient Ctrl CAM2 s height position can be controlled in Z axis Tip amp Recipient Ctrl Calibration Position sets the Camera2 calibration height Recipient hole searching height is set at Recipient Search Position The value can be changed using NumPad and saved when Set button is clicked Safety zone is to prohibit X Y rotate moves at
27. ection to MAIN POWER SUPPLY a For plug connected instrument only Where protective ground is required plug the instrument into a supply outlet which has an earth connection b For PERMANENTLY CONNECTED INSTRUMENT only Do not use the instrument until it has been installed by a qualified electrician or authorized service engineer 3 Unplug the instrument immediately after use 4 Unplug the instrument before filling with liquid 5 Do not place the instrument in liquid nor put it where it could fall into liquid If the instrument becomes wet unplug it before touching it 6 Do not leave the instrument unattended while it is plugged in 7 Use the instrument only for the purpose described in the instructions for use 8 Do not use accessories which are not supplied or recommended by the manufacturer 9 Do not use the instrument if it is not working properly or if it has suffered any damage NOTE Examples of physical damage include a damage to the flexible supply cord or its plug b damage caused by dropping the instrument c damage caused by dropping the instrument into water or splashing water onto it 10 Do not let the instrument or its flexible cord come into contact with surfaces which are hot 11 Do not block air openings or place instrument on a soft surface which might block them and keep air openings free from lint hair fluff etc 12 Do not place anything on top of the instrument 13 Unless specifically i
28. ed by UNITMA which has been attached to or used in connection with the product 4 Customer must provide proof of purchase bearing the date of purchase and the product s serial number in order to receive warranty service Warranty service will be provided by UNITMA through one of its authorized warranty service centers 8 2 The Warranty does not cover 1 Defects or damage resulting from the use of the product other than its normal customary use 2 Defect or damage from misused such as breakage spillage of liquid or substances that can produce circuit shortcut of the electronic boards and battery leakage etc 3 Defect or damage from improper testing operation installation alternation modification or adjustment of the instrument 4 Breakage or damage unless caused directly by the defects in material workmanship 5 A product with unauthorized product modifications dissembles and or repairs which adversely affect the performance of the product 6 Product which has had the serial number and the warranty seal removed or made ineligible 7 Scratches or other cosmetic damage to the product that do not affect the operation 8 Normal and customary wear and tear 9 Force Majoure any damage beyond the reasonable control of Unitma such as but not limited to Fire Flood Earthquake act of Terrorism etc 10 Shipping costs for pick up and delivery from your facility 8 3 Contact for technical service Please contact UNITMA CO LTD f
29. ed by the needle of tip when assembling or disassembling the tip body or the block holder frame or when cleaning the interior of the instrument or the tip e Be careful not to pinch your fingers when closing the front door of the instrument 5 2 1 Place donor blocks and a recipient block on the block platform in Quick Ray Master 5 2 1 1 Assembling the recipient block into the recipient block holder 1 Put the recipient block into the recipient block holder top 2 Insert the recipient block holder top with the recipient block into the recipient block holder 3 Assembled recipient block 5 2 1 2 Assembling the recipient block holder into the block holder frame Insert the assembled recipient block holder into the block holder frame 5 2 1 3 Assembling the donor block into the block holder frame Repeat the above steps 5 2 1 2 with donor blocks and insert into the block holder frame 28 S3 UNITMA Quick Ray Master UATM 272 Operating Manual 5 2 1 4 Assembling the block holder frame into the instrument 1 Turn on the system 2 Start Auto Mode 3 Load spread sheet file and click on Set Tray button 4 Open the tray door 5 Assemble the block holder frame into the jig for the block holder frame of the instrument e The jig for the block holder frame of the instrument can not be disassembled from the instrument Do not disassemble the jig from the instrument lt Remove Donor Block Ho
30. eserved Licensed products are owned by Unitma Co Ltd and are protected by national copyright laws and international treaty provisions Unitma Co Ltd products are covered by patents issued and pending Specifications and price change privileges reserved Quick Ray is registered trademarks of Unitma Co Ltd All Information is subject to change without prior notice Contacting Unitma Co Ltd UNITMA Co Ltd 3F Chungmyeong B D 224 8 Jamsil Dong Songpa Ku Seoul 138 220 Korea Tel 82 2 420 0070 Fax 82 2 420 9797 http www unitma com E mail unitma unitma com S3 UNITMA Quick Ray Master UATM 272 Operating Manual Table of contents 1 IMPORTANT NOTES 1 1 Explanation of Symbols used 1 2 Name plate 1 3 Qualification of personnel 1 4 Intended use of instrument 2 SAFETY NOTES 3 INSTRUMENT COMPONENTS AND SPECIFICATIONS 3 1 Instrument description 3 2 Instrument component 3 3 Instrument specification 3 4 Consumable Recipient block 4 SETUP 4 1 Installation site requirements 4 2 Setup 4 3 Connecting to main power supply 4 4 Turning on off the instrument 5 GENERAL PROCEDURE OF TISSUE MICROARRAY 5 1 Building TMA with Quick Ray Master 5 2 Preparation for punching and inserting with Quick Ray Master 5 3 Procedures for punching and inserting with Quick Ray Master 5 4 Auto run Operation 5 5 Manual Operation 5 6 Set Mode for Calibration 5 7 Miscellaneous Buttons 5
31. f recipient blocks The user can also save Us 99 UNITMA Quick Ray Master UATM 272 Operating Manual image files and report files through an external USB memory and an external PC with LAN interface 3 2 Instrument components Front side View 1 Monitor 2 Cover Door 3 X axis frame 4 Jig for block holder frame 5 Block holder frame removable parts 2 pcs provided 6 Tip body holder 7 Camera 1 8 Camera 2 9 Illumination 0 10 Door Sensor k UNITMA Quick Ray Master UATM 272 Operating Manual Right side View 11 Main power switch 12 PC POWER switch 13 Monitor connector DVI type 14 USB connector 15 LAN connector 14 S3 UNITMA Quick Ray Master UATM 272 Operating Manual Setting up Tray 16 Donor block 17 Recipient block 18 Recipient block holder top removable part 1pc provided 19 Recipient block holder body removable part 1pc provided weights 30g 20 Block holder Tray removable part weights 300g ae 21 Tip body weights 80g 22 Tip needle 23 Tip 99 UNITMA Quick Ray Master UATM 272 3 3 Instrument specification Approvals Nominal supply voltages Nominal supply current Nominal frequency Weight Max size WxDxH Operating temperature range Operating Manual UL CE ISO Selectable by service personnel 100 to 120 VAC 200 to 240 VAC Selectable by service personnel 8A 1 5A 0 3 kW 50
32. gurations is provided e Never disassemble the instrument or supplied accessories Disassembling will void the instrument warranty Additionally there is a risk of fire electrical shock or malfunction e Do not use accessories that are not supplied or recommended by UNITMA e Contact UNITMA for repairing service or to purchase accessories Do not use the donor block or a sample with a biological hazard or a chemical hazard e Do not use the donor block or a sample with biological hazardous substances and or chemical hazardous substances that can impact human health Handle biohazards e Working with infectious material requires the following precautions a Limit access to areas where experiments with infectious specimens are in progress b Clearly label areas where biohazards are in use and designate specific areas where biohazards are routinely used using this symbol black on red background WZ c Wear lab coat gloves and safety glasses to prevent contamination from the infectious specimen and remove them when leaving the work area d Decontaminate work surfaces once per day and after any spill of viable specimen e Eating drinking and applying cosmetics are not permitted in the work area f Wash hands after handling viable specimens before leaving the lab g Transport contaminated materials in leak proof containers clearly marked with biohazard labels me 29 UNITMA Quick Ray Master UATM 272 Bio
33. hazard Determination e Lab Bio safety Level BSL Criteria Operating Manual The following guidelines can be used by all laboratory personnel a Bio safety Level 1 is appropriate for undergraduate and secondary educational training and teaching laboratories and or other facilities in which work is done with defined and characterized strains of viable microorganisms not known to cause disease in healthy adult humans b Bio safety Level 2 is applicable to clinical diagnostic teaching and other facilities in which work is done with the broad spectrum of indigenous moderate risk agents present in the community and associated with human disease of varying severity c Bio safety Level 3 is applicable to clinical diagnostic teaching research or manufacturing facilities in which work is done with indigenous or exotic agents where the potential for infection by aerosols is real and the disease may have serious or lethal consequences d Bio safety Level 4 is applicable to work with dangerous and exotic agents which pose a high individual risk of life threatening disease for which there is no vaccine or other treatment available e Practices and Techniques Safety Equipment Bio safety Practices and Safety Equipment Standard microbiological practices Level 1 practices plus Laboratory coats decontamination of all infectious wastes limited access protective gloves and biohazard warning signs
34. id biohazard bags are to be tied 4 Red biohazard bags are to be placed for storage handling and transport in rigid containers with tight fitting lids labeled with the words Bio hazardous Waste or the word Biohazard and the international biohazard symbol on the lids and sides so as to be visible from any lateral direction 5 PATHOLOGY WASTE All pathological wastes must be separated from other medical hazardous wastes Waste will be placed in a red bag and deposited into a specially marked secondary container labeled with words Pathology Waste or PATH The container will be stored in the morgue refrigerator until pick up by authorized medical waste hauler for transportation to an approved incineration facility Once specimens or tissues are deemed waste they shall not be stored for more than seven 7 days at a temperature above 32 degrees Fahrenheit Disposal of Tip waste 1 The tip waste must be contained in a special sharp object container 2 The waste with specimen shall be placed in a red biohazard bag n UNITMA Quick Ray Master UATM 272 Operating Manual 7 TROUBLE SHOOTING 7 1 Instrument failures Some failures or malfunctions of the instrument are indicated by the applicative software as error message Follow the software instructions to solve the problems and take corrective actions 7 2 Operating error with possible causes and corrective action no Error message It is Not selected
35. ion is defined as below UNITMA Quick Ray Master UATM 272 Operating Manual So unrrma Quick Ray Maen Click on the pictured icon HHAB the camera will move to the donor block1 position X Y position value in mm can be modified and set Click on the number NumPad will popup for numeric input NumPad Position X 36 2135 36 2135 mesacna et te p g nt 4 r Type in the number and click on OK button at the NumPad To save the set value click on SET button on the right side of values 5 6 2 Recipient Block Parameter Click on Recipient Block Param and below screen will appear Recipient block position is defined as shown below j J i RERRERRE 1 Standby Pos Set Tray Pos The tray position for donor recipient block change can be set By 99 UNITMA Quick Ray Master UATM 272 Operating Manual clicking the button tray moves to the defined position and the value can be modified using NumPad After modifying the value click on the Set button to save the value 2 Ready Pos Set the value for Tip change position Click the Ready Pos button to move the Tip to the defined value Modified the value using NumPad and click on SET button to save the value 3 Cal Pos 1mm 2mm 3mm 5mm Set the value for Calibration position for Camera2 Click the button to move to the position shown below and modify the value as necessary by using N
36. is calculated 7 Move CAM1 Calibration Pos Cam1 moves to the Cal Pos Donor in the Recipient Block Param to the chosen tip size at Select Model UNITMA Quick Ray Master UATM 272 Operating Manual Suita Quick Ray Master UATM 272 file Pea 8 Calibrate CAM1 Camera s resolution in pixel is calculated The camera moves x and y position by both positive and negative 0 5mm and compare the mechanical movement with display and find the resolution and centers it This is to measure the distance between the Tip and Cam1 9 Register CAM1 Distance Distance between the tip and Cam2 is calculated 10 SAVE This button saves the all the measured data for the program Back button also saves the data If user clicks on Main Menu button at the sub menu screen the data will not be saved 5 6 6 Motion amp IO Property Click on Motion amp IO Property button to see the display below The motor set values can be edited and sensor status I O can be monitored Refer to the appendix for Motion OCX and I O OCX Swm Quick Ray Master UATM 272 ii UNITMA Quick Ray Master UATM 272 Operating Manual Sunma Quick Ray Master UATM 272 as se iit Le 5 6 7 Setting orn Quick Ray y behect Domar Merk Coder Whe Mak ed Donor Mark Select the marking color for setting Search Select donor mark color and press search button As shown below Donor I
37. k stage into the stage holder Donor blocks should be made from paraffin embedded tissues and recipient block should be just only UBO6 supplied and made by UNITMA Co Ltd 2 The user will see images of donor blocks and recipient blocks on a built in LCD monitor and can use the touch screen to adjust settings and run the UATM 272 Users can select and save the specific position for extraction of tissue core on the selected donor block image and the specific hole of corresponding recipient block by clicking the block image and button image of the touch screen 3 When the user clicks the RUN button in software program for the TMA procedure UATM 272 executes the TMA block construction process as programmed by the user 4 The user can print the TMA report generated in UATM 272 with an external printer and save the image files of the donor blocks and the recipient block into a USB memory stick e The use of the instrument for any other purpose than originally intended may damage the instrument or injure the user and will void all warranties 23 UNITMA Quick Ray Master UATM 272 Operating Manual 2 SAFETY NOTES Read the following safety notes to avoid injury and prevent damage to this product or any products connected to it Misuse of electrical equipment can cause electrocution burns fire and other HAZARDS READ THIS BEFORE USING THE INSTRUMENT 1 Check that the voltage setting matches the supply voltage 2 Conn
38. k holder frame from the jig for block holder frame of the instrument 4 Assemble the new block holder frame into the jig for block holder frame of the instrument 5 Close the cover door Replacing recipient block holder 1 Wait until the operation of the instrument is finished and stopped 2 Open the cover door 3 Disassemble the block holder frame from the jig for block holder frame of the instrument 4 Disassemble the recipient block holder from the block holder frame 5 Put the new recipient block holder with the new recipient block into the block holder frame 8 Assemble the block holder frame into the jig for block holder frame of the instrument 9 Close the cover door 6 4 Maintenance 6 4 1 Checking the instrument UNITMA recommends that the instrument be checked by the user at least once every six months or whenever you transport the instrument Check Exterior amp Interior e Clean as the instruction 5 1 Cleaning the instrument e Check for readability or missing labels Check the cord and molded connectors for damage Legs e Check for presence of all four legs e Check legs for physical damages or deteriorations Power cord receptacle e Make sure receptacle is free of foreign matter e Check for presence of all four connector pins and make sure they are straight Run Demo mode e Turn on the instrument and run Demo mode e See Error message 59 23 UNITMA Quick Ray Maste
39. lative humidity non condensing Operating 20 to 60 i UNITMA Quick Ray Master UATM 272 Operating Manual Storage 20 to 60 Temperature Operating 10 C to 35 C 50 F to 95 F Storage 10 C to 40 C 50 F to 104 F No direct sunlight Do not use an extension cord A e Do not operate the instrument in rooms where possible accumulation of gases may cause explosion or explosive materials are stored ee 99 UNITMA Quick Ray Master UATM 272 Operating Manual 4 2 Setup 4 2 1 Assembling the tip body into the instrument e Tip bodies are assembled when you receive the instrument If the Tip needle becomes damaged or looses sharpness you need to change them as the following instructions e The tip body should be assembled into the correct position in the tip body holder of the instrument 1 Turn off the instrument 2 Open the cover door 3 Assemble the tip body into the tip body holder of the instrument 4 Close the cover door 4 2 2 Interfacing with external devices e Any electrical external devices interfaced with the instrument should be connected to ground External device list 1 Monitor with a DVI port 2 Mouse with an USB port 3 Keyboard with an USB port 4 USB Memory stick 5 Printer with an USB port 6 PC with an LAN port 99 UNITMA Quick Ray Master UATM 272 Operating Manual Interfacing with external devices e Interf
40. lder gt lt Remove Recipient Block Holder gt lt Fill the holders and put them back on the stage gt a UNITMA Quick Ray Master UATM 272 Operating Manual 6 Close the cover door 5 2 2 Disassemble the recipient block from the Quick Ray master 1 Wait until the operation of the instrument is finished or stopped 2 Open the cover door 3 Disassemble the block holder frame from the jig for block holder frame of the instrument 5 Disassemble the recipient block from the recipient block holder 4 Disassemble the recipient block holder from the block holder frame 6 Once recipient block is disassembled return the block holder frame into the instrument 7 Close the cover door is 99 UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 Procedures for punching and inserting with Quick Ray Master 5 3 1 Run the application software of Quick Ray Master 1 Connect the AC power cord to the instrument and then to the AC power source outlet 2 Turn on the Main power switch 3 Push on the PC power button 4 The Quick Ray Master software will start and display Main Menu SE UNITMA Quick Ray 5 3 2 Click on AUTO from the Main Menu screen 1 Select Auto button to display Auto mode screen Initialization process will automatically start S UNITMA Quick Ray 2 Click on Load File button at the Auto mode F unma Quick Ray Loading ea EZ Select G
41. message will be displayed From Auto Mode screen touch Measure button for the instrument s height gauge measures the height of each block and can run the program Check recipient block amp tool To run the program you need to visually check that both the size of the tool s tip and Recipient block matches You need to confirm that it is the correct size to run the program Front door is opened Please In order to run the program the cover close the door door must be closed Please check the door is closed and run the program ine 99 UNITMA Quick Ray Master UATM 272 Operating Manual 8 WARRANTY amp SERVICE UNITMA CO LTD UNITMA has limited warranty for the Quick Ray Master against defects in material and workmanship under normal use and service for period of 1 year from the date of purchase 8 1 Terms amp Conditions 1 UNITMA will at no charge either repair the Quick Ray Master with new parts or replace it with a new unit during the warranty period provided it is returned in accordance with the term of the warranty Replaced parts are warranted for the balance of the original applicable warranty period All replaced parts of the products shall become property of UNITMA 2 This limited warranty is extended by UNITMA to the original end user purchaser and is not assignable or transferable to any other third party 3 UNITMA cannot be responsible in any way for any additional equipment not furnish
42. nfo is displayed live Use slider or press to find the best etting for the marking identification process his process will help the unit to correctly identify the marking on the donor block al UNITMA Quick Ray Master UATM 272 Operating Manual Registration Recipient hole searching size to be defined S5 unrrma Quick Ray eeoeee a eb ie aar te a ee ee eH ee 1 ee image File Load SS Rag Model BinatHisto Blob amp Track Results Model No 0 100 0 ADO Model Size Widths Pow H aT Alaje Col pa Rw o Wide EE nig E Set Setting A e o _ ary Set button in Mark Size defines the hole searching size ROI defines he hole searching area Bina amp Histo tab shows the histogram of current image and can be adjusted to easily identify the hole Blob amp Track tab let you test the recipient hole searching Result tab shows marking location data In case hole searching fails often this process may improve the earching activity Search Select Donor Mark Color Q Wee Black Red Recipient Search Setting Dhegsteation aati o Move lt A J cor N 1 12 he green colored hole identification mark can be adjusted with keys 3 UNITMA Quick Ray Master UATM 272 Operating Manual 5 6 8 Exit SETUP There are two ways to exit the program To exit the program click on the EXIT button on the top menu Sunma Q
43. nstructed to do so by this operating manual do not drop or put anything into any opening in the instrument or into any hose or coupling 14 Do not use the instrument where aerosol sprays are being used or where oxygen is being administered 15 Do not use the instrument out doors 23 UNITMA Quick Ray Master UATM 272 Operating Manual To avoid fire and the instrument failure e Observe all warnings and instructions e Observe all Terminal Ratings To avoid fire or shock hazard observe all ratings and markings on the product Consult the product manual for further ratings information before making connections to the product e Power Disconnection The power switch disconnects the product from the power source See instructions for the location Do not block the power switch it must remain accessible to the user at all times e Regularly inspect the AC power cord for damage and for dust build up around the power plug or electrical outlet e Stop using and unplug the AC power cord from the electrical outlet and disconnect any other cables immediately in the following cases when the instrument functions in an abnormal manner produces unusual sounds if there is unpleasant or offensive odor and becomes too hot to touch e Do not operate when the cover door of the instrument is opened Close the cover door before operating this instrument e Do not operate with suspected failures If you suspect that there is damage to the
44. omer Berk Color Oar Bie FAH A Select Aecipoanl Bimi om Bip Gaede ewm i UNITMA Quick Ray Master UATM 272 Operating Manual e When Load File button is pressed User should provide a spread sheet file in MS Excel format The spread sheet should contain Donor block and process information such as core size to puncture number of donor blocks sample counts for each block and donor block ID 5 3 3 Click on Load File button for the job The Data file should be in MS Excel format and contains information such as donor block ID Core size in mm Number of donor sample blocks and sampling count for each block The format and examples are stored in HDD for reference 5 3 4 Select Donor marking color lt is recommended to use the marking color that can be easily spotted among the donor blocks Quick Ray Master will scan and search the selected donor marking color from the block and will extract from the recognized area 5 3 5 Select recipient block Select which recipient block s is are to be used Select ALL to use both A and B recipient block Select COPY if samples from each donor block to be distributed in both recipient blocks The loaded spread sheet should have the punch count in even number Select RecipA or RecipB if only the selected donor block is to be used Recipient A and B markings are engraved on the tray 5 3 6 Set Tray Set tray button will slide the Stage to the front where the Tray can
45. or technical service ee 99 UNITMA
46. ow about tissues for immunohistochemistry in situ hybridization FISH and in situ PCR e Quick Ray Master UATM 272 The instrument Quick Ray Master UATM 272 is an automated tissue microarrayer for extracting the tissues from donor blocks and subsequently delivering them into the recipient block The recipient block the patented technologies of UNITMA is made of special materials that melts when heated at 60 C for 30 minutes and has pre made evenly spaced round wells arranged in a square matrix Therefore using UNITMA recipient block will save the current block building time and guide the pathologists to fully utilize the power of TMAs The instrument uses these ready to use pre made recipient blocks helping to decrease the time and labor needed for block creation In addition because it has one tip module that holds 4 tips hole size 1mm 2mm 3mm 5mm which can automatically be changed simply by using the software there is no need for disassembling or assembling punch modules when changing the hole size The instrument provides an automatic mode for quick and easy use and a semi automatic mode for manual user The instrument has the built in computer running by MS Windows XP OS and a LCD monitor with a touch screen and is also connected to an external monitor USB keyboard and USB mouse Also the quick help function key is available for various tips including instructions on saving image files and reporting the information o
47. r UATM 272 Operating Manual 6 4 2 Replacing the fuse e Only use a fuse of the same specification For the required value please refer to the fuse specification in this manual e Unplug the AC power cord Removing or touching the fuse with the AC power cord plugged will expose you to hazardous voltages and cause death 1 Locate the fuse holder module It is located as part of the power cord assembly on the back of the instrument 1 Fuse panel notch 2 Fuse holder 3 Fuse holder module 2 There is a notch at the bottom of the fuse panel where you can insert a flat head screw driver or some other flat object Use leverage to open the fuse panel as it opens upward from the bottom 3 Pull out the fuse holder The fuse holder has a fuse 4 Remove the old fuse by gently pulling it out 5 Insert the new fuse into the fuse holder 6 Slide the fuse holder back into the fuse holder module 7 Snap the cover of the fuse panel 6 5 Repair A e Please contact UNITMA for repairing services replacement components and additional consumables ee UNITMA Quick Ray Master UATM 272 Operating Manual 6 6 Disposal of waste Disposal of waste from donor block or recipient block with tissue specimen 1 The waste with tissue specimen should be contained and handled separately from other wastes at the point of generation 2 The waste with specimen shall be placed in a red biohazard bag 3 R
48. uick Ray Master UATM 272 Ea Or Click on the Exit button on the title bar at the auto menu 2T UNITMA icare Quick Ray Do not click on the exit button while program is running under auto mode Paused program is also considered as on processing status If program is not completed click on the suspend button to enter into exit ready status 5 7 Miscellaneous buttons Vision screen panel Vision screen shows the image of the recipient block or the donor block lt Vision screen gt Zoom button Click the Zoom button to change zooming on the vision screen c Zooming In the vision image scale ZOOM Zoom In UNITMA Quick Ray Master UATM 272 Operating Manual Zooming out the vision image scale ZOOM Zoom Out Camera Button Click the Camera button to control the camera IE rah Grab E m the Live button to select live mode of the camera Live Live Click the Grab button to snap shot the camera image 99 UNITMA Quick Ray Master UATM 272 Operating Manual 5 8 Report Click the REPORT button to write the results of TMA process The user can input the label or report about the recipient block using the keyboard and save the information as a Microsoft Office Excel file format Sunma Quick Ray Master UATM 272 tur 8 7 A A i A i hed oenetor Legh Light 200m 200m Grab Lives Open Save Crganigation Position
49. umPad Click on SET button to save the value ii UNITMA Quick Ray Master UATM 272 Operating Manual When Cal Pos button is pressed Cam2 moves to the calibration position as shown above and displays the screen below umma Quick Ray Master UATM 272 ii r far cmt uve Seal neh Cam2 screen should point embossed marking on the crossing center Tip Let the marking position set on the center but slightly tilted to the upper right position 4 Cal Pos Donor Set value and calibrate Camera1 Set camerai with 1mm tip other tips 2 3 5mm are approximately 10mm apart and automatically set When Cal Pos Donor button is clicked the camera moves the position The set value can be entered from NumPad and click on Set to save the value 99 UNITMA Quick Ray Master UATM 272 Operating Manual When Cal Pos Donor button is pressed Cam1 moves to the calibration position as shown above and displays the screen below ssumnea Quick Ray Master UATM 272 l Cal ie oe p iT Center of Cam1 should point 1mm mark 5 Cal Pos Tip To set the distance between camera and tip position tip should be located at the center of calibration mark By clicking the Cal Pos Tip button the tip will be located The position can be tuned with touch pad screen and click on Set button to save the value When Cal Pos Tip button is pressed Tip moves to the calibration position as shown a
50. ve Ground or Earth Terminal Used to indicate a circuit common BIOHAZARD WARNING BIOHAZARD WARNING indicates an injury or hazard not immediately accessible as you read this symbol Bio hazard Infectious agent A type of micro organism bacteria mold parasite or virus which normally causes or significantly contributes to the cause of increased morbidity or mortality of human beings S3 UNITMA Quick Ray Master UATM 272 Operating Manual 1 2 Name plate The name plate of UATM 272 is at the Model Name UATM 2 right side of UATM 272 The name plate SESS BU Ee Selectable by service personnel indicates the serial number year of O 100 to 120 Y 3 04 50 60Hz O 200 to 240 Y 1 54 50 60Hz manufacture and the power rating and Eora eaaa SERIAL No DATE OF MANUFACTURING MAUNFACTURED BY UNITMA CO LTD LISTED MADE IN KOREA www unitma com frequency 1 3 Qualification of personnel The Quick Ray Master UATM 272 should be operated by trained Histopathology laboratory personnel All laboratory personnel designated to operate UATM 272 is required to read this entire manual before operating UATM 272 1 4 Intended use of instrument The Quick Ray Master UATM 272 is an automated Tissue Microarrayer for extracting the tissues from donor blocks and subsequently delivering into recipient blocks 1 The user inserts donor blocks and a recipient block into the block stage and puts the bloc

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