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PS 503 XL - Lambda Life as

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2. sjuawbel4 pue 186 esoJeBe siseJoudou15819 VWNY VNG Aniqneduwos 491nduJo ZEZ7Sy S9U21IMS 911122 Ag sBunieg EEEE o EE 4J0 e169 u J9AES 1 E J9MOd pue Jue SUNN juaJ4n2j pue abe zOA 5 02 00 M J9Mog WnUIXEN 00 3u8J4n2 110 J QO QO D D A LL 2 lt LL I LL ME S gt C2 owes SUPPLIES COMPACT LIGHT WEIGHT AND POWERFUL UNITS PS 304 XL Cat No 160600 300 Volts e 400 mA PS 503 XL PS 305 XL Cat No 172500 Cat No 170500 500 Volts e 300 mA 300 Volts 500 mA Timer and GEL SAVER Timer and GEL SAVER GEL GEL SAVER SAVER PS 304 XL PS 503 XL PS 305 XL Voltage Output range Resolution 1 300 V 1 volt steps 1 500 V 1 volt steps 1 300 V 1 volt steps Current Output range Resolution 1 400 mA 1 mA steps 1 300 mA 1 mA steps 1 500 mA 1 mA steps N 0 999 1 min steps 0 999 1 min steps P C Maximum Power 100 W 100 W 100 W lt Minimum regulated value 1 volt 1 mA 1 volt 1 mA 1 volt 1 mA cO LLI Minimum non regulated value 1 volt 15 pA 0 3 W 1 volt 15 pA 0 3 W 1 volt 15 pA 0 3 W Un Fault detection stop audible alarm message stop audible
3. 5 B8 B BANOP Janetta Tar a P s s s 0 0 0 0 0 b 0 0 0 000000000040 0 0 TWWWWWWWWWW 9 e 8 5 5 5 5 8 M o e e o n 0000000 N N s e 0 0 00 0 0 86 6 1 4 4 9246 644642060 ee eee 6686 ooo 0 0 0 0 0 0 0 0 ove ee ee ee XX an a 0 0 MMe PrP eee pe e INFORMATION FOR MORE H s 0 x eee ene ero 0 0 0 0 0 0X 000004000000 00 00 000000000000 sg gege ee e eeeeeeeee ee ee ee aa e 0 00 0 0 0 0 0 0 0 0X 9 O pg ee ee e ee oa 5 2 50 0 0 0 0 0 D mt e D o pee ae 0 0 0 0 92020 poe 75 76 Base 9 nue i 600 Op e w j o gt Q NC V oux P 29 06 0009 a OQ gt oo AN e Bee o o 9 90 b eee 05 0000 Z M diss e ZR EN NM NN MN ecd cd LJ 20000000 le dca 0000 00 XX 2 a a 000000060 00000000 x e e EEE ee Ze 0 Zeep X x 00000 0000000600 000000000000 0 s a
4. Ten Minute Western Blot 2940 to 20500 Markers Amido Black Stained A 30 Minute Southern Blot m of Human DNA Sc Loading Position SUGGESTED POWER SUPPLIES PS 305 XL 300 Volts 500 mA Cat No 170500 PS 304 XL 300 Volts 400 mA Cat No 160600 Blotting Position 43 T BLOTTING SEMI DRY BLOTTING 450000 Mini Semi Dry Blotter for gel up to 11 x 11 cm Protein DNA RNA Membrane Nitrocellulose 460000 Large Semi Dry Blotter for gel up to 20 x 20 cm 0 45um or 0 2um PVDF 0 45um or 0 2um Transfer Buffer Towbin Buffer 3 Buffer 0 5X 1XTBE System Bjerrum NAQ Mini and Large Semi Dry Blotters 2 sizes 11 x 11 cm amp 20 x 20 cm e Very high conductivity with corrosion free electrodes e Cathode in stainless steel anode in platinum plated titanium for long life e Up to 3 mA cm of gel provides a very fast transfer 10 14 Volts maximum 10 14 Volts max e Strong side clamp system provides an uniform pressure between the gel and the transfer membrane Schafer Nielsen Power Setting Constant current Constant current 0 8 3mA cm 0 5 3mA cm gel surface area gel surface area Running Time 30 minutes to 2 hours 30 minutes Needs to be experimentally to 2 hours determined large molecules generally in need longer transfer time the lower Ideal for proteins less than 150 kd Fast WESTERN BLOT Mini Gels in 15 minutes Designed for extensive use
5. e Maximum number of samples 392 using 8 combs compatible with multichannel pipettes e Gel tray 20 x 24 cm has 8 spaces for combs insertion Gel tray 20 x 12 cm has 2 spaces for combs insertion ONE FREE GEL TRAY WHEN ORDERING 2 ADDITIONAL COMBS preparative models Double combs 44 21 teeth Equipped with cooling compatible with multichannel built in channel for pipettes buffer temperature e 4 adjustable feet and bubble level control that can be e Ports for buffer circulation and maintained by circulating cooling for temperature control water through the base Cooling ports for temperature Buffer Volume 1 800 ml control Unit dimensions Buffer circulation ports x Lx h 28 x 37 x 9 cm ORDERING INFORMATION 331000 MAXIGEL THERMO 2 Includes Removable U V transparent gel tray 20 x 24 cm w x 1 2 removable casting gates 1 0 mm thickness double sided comb 44 21 teeth sample volume 14 24 pl Cat No 334421 Compatible with multichannel pipettes safety lid set of power leads buffer and cooler circulation accessories user s manual 3310PG PACK G Includes MAXIGEL THERMO 2 Fully equipped as above Cat No 331000 Power supply PS 305 XL Thickness Teeth Sample volume Well width 330101 Comb 1 0 mm 10 64 ul 16 0 mm 330102 Comb 2 0 mm 10 128 ul 16 0 mm 330103 Comb 3 0 mm 10 192 ul 16 0 mm 330201 Comb 1 0 mm 20 26 ul 6 5 mm 330203 Comb 3 0 mm 20 78 ul 6 5 mm 330301 Comb 1 0 mm 30 16 ul 4
6. 4 9 E 1j d 400000 400615 400815 400915 401015 401215 40SP15 400608 400808 400908 401008 401208 40SP08 401010 401012 401020 401025 400010 400012 401035 400020 400021 401030 480000 481015 481515 482015 482415 4815PREP 48SP15 481008 481508 482008 482408 48SP08 481614 481615 481025 480010 480012 481035 480020 481030 PROTEINES VERTIGEL 2 MiniVertigel 2 compl te pour 2 gels de 9 0 x 8 5 cm 1 x H paisseur 0 8 mm base de coulage de gel et joints de remplacement 2 plaques de verre avec encoche 4 plaques de verre pleines 10 x 10 cm 2 plaques c ramique 1 paroi d obturation 2 peignes 10 dents 0 8 mm 4 espaceurs 0 8 mm couvercle avec cordons solidaires 2 joints de remplacement notice Peigne 6 dents 1 5 mm volume des puits 266 ul Largeur des dents 11 mm Peigne 8 dents 1 5 mm volume des puits 142 ul Largeur des dents 7 7 mm Peigne 9 dents 1 5 mm compatible pipettes multicanaux volume du puit 138pl Peigne 10 dents 1 5 mm volume des puits 134 pl Largeur des dents 5 7 mm Peigne 12 dents 1 5 mm volume des puits 103 pl Largeur des dents 4 3 mm Espaceurs 1 5 mm x 2 Peigne 6 dents 0 8 mm volume des puits 142 ul Largeur des dents 11 mm Peigne 8 dents 0 8 mm volume des puits 99 ul Largeur des dents 7 7 mm Peigne 9 dents 0 8 mm compatible pipette multicanaux 80 ul Peigne 10 dents 0 8 mm volume des puits 73 ul Largeur des dents 5 7 mm Peigne 12
7. Volume acrylamide pour 2 gels 15 ml Courant constant 15 30 mA par gel e Voltage de 150 a 300 Volts e Temps de migration 30 a 60 minutes CARACTERISTIQUES Volume total de tampon 300 400 ml Nombre Maximum d chantillons 2x12 Dimensions de la cuve Hx lx P 14 x 17 x 12 cm Peignes et accessoires Pages 94 95 MINI VERTIGEL 2 PC R f 400800 Pour gels pr coul s 10 x 10 cm Pages 46 51 CUVES POUR PROTEINES BH GENERATEURS CONSEILLES Page 62 ila APELEX PS 304 XL APELEX XL gt x z 5 305 ee ER O e i A gt wO ws PS 304 XL R f 160600 PS 305 XL R f 170500 CUVES DE TRANSFERT CONSEILLEES Pages 98 99 1 Mini Transblotter R f 430000 2 Slim Blotter Ref 504001 3 Mini Semi Dry Blotter Ref 450000 PACK I Autres packs page 102 e MINI VERTIGEL 2 Ref 400000 e MINI TRANSBLOTTER Ref 430000 e PS 304 XL Ref 160600 e MINI VERTIGEL 2 Ref 400000 7 e MINI TRANSBLOTTER Ref 430000 0 PROTEINES MINI WIDE VERTIGEL 2 CAPACIT MAXIMUM 50 CHANTILLONS AVEC 2 PEIGNES DE 25 DENTS DESCRIPTION Dimensions utiles des gels 19 x 8 5 cm L x H e Plaques de verre 20 cm x 8 10 cm Hauteur 410000 MINI WIDE VERTIGEL 2 Volume acrylamide pour 2 gels 32 ml Livr e compl te avec accessoires pour couler et migrer 2 gels e Courant constant 15 45 mA par gel de 0 8 mm d
8. providing ease of use and precise results 65 bp Gi sibp 990 1 36 bp 1 000 n d ANAMED PROGEL HOMOGENIOUS TRIS GLYCINE GELS LAEMMLI GELS FOR PROTEIN ANALYSIS TG 04110 TG 04112 TG 04115 TG 04510 TG 04512 TG 04515 TG 06110 TG 06112 TG 06115 TG 06510 TG 06512 TG 06515 TG 08110 TG 08112 TG 08115 TG 08510 TG 08512 TG 08515 TG 10110 TG 10112 TG 10115 TG 10510 TG 10512 TG 10515 TG 12110 TG 12112 TG 12115 TG 12510 TG 12512 TG 12515 TG 14110 TG 14112 TG 14115 TG 14510 TG 14512 TG 14515 TG 16110 TG 16112 TG 16115 TG 16510 TG 16512 TG 16515 TG 18110 TG 18112 TG 18115 TG 18510 TG 18512 TG 18515 IDEAL PERFORMANCE BETWEEN 4 Tris Gly Gel 1 0 mm 10 well 4 Tris Gly Gel 1 0 mm 12 well 4 Tris Gly Gel 1 0 mm 15 well 4 Tris Gly Gel 1 5 mm 10 well 4 Tris Gly Gel 1 5 mm 12 well 4 Tris Gly Gel 1 5 mm 15 well IDEAL PERFORMANCE BETWEEN 6 9o Tris Gly Gel 1 0 mm 10 well 6 9o Tris Gly Gel 1 0 mm 12 well 6 9o Tris Gly Gel 1 0 mm 15 well 6 9o Tris Gly Gel 1 5 mm 10 well 6 9o Tris Gly Gel 1 5 mm 12 well 6 9o Tris Gly Gel 1 5 mm 15 well IDEAL PERFORMANCE BETWEEN 8 Tris Gly Gel 1 0 mm 10 well 8 Tris Gly Gel 1 0 mm 12 well 8 Tris Gly Gel 1 0 mm 15 well 8 Tris Gly Gel 1 5 mm 10 well 8 Tris Gly Gel 1 5 mm 12 well 8 Tris Gly Gel 1 5 mm 15 well IDEAL PERFORMANCE BETWEEN 10 Tris Gly Gel 1
9. Heavy lid with Stainless Steel Cathode Plate Mini format for 10 x 10 cm gels Base with Platinum coated Titanium Anode Plate Heavy duty knobs Large gels up to 20 cm x 20 cm or four gels 10 x 10 cm TANKS FOR BLOTTING WHAT TANK FOR BLOTTING Solution 2 LIQUID BLOTTING GEL TRANSBLOTTERS MINI AND LARGE e Separate cassettes give flexible gel capacity e Designed for NORTHERN SOUTHERN AND WESTERN BLOTTING Solution 1 Semi Dry BLOTTING e Recommended for BLOTTING OF PROTEINS AND NUCLEIC ACIDS Ideal for transfering all Protein overall less than 150 kd e No significant volume of buffer to prepare Solution 3 LIQUID BLOTTING SLIM BLOTTERS e ow buffer volume e Very fast tranfer ow voltage e 5 tank sizes for Western Blotting e 5 tank sizes equipped by platinized Titanium anode for NORTHERN SOUTHERN AND WESTERN BLOTTING 9 Dacko coplas s 0 e MINI VERTIGEL 2 Cat No 400000 e MINI TRANSBLOTTER Cat No 430000 e PS 304 XL Cat No 160600 e MINI VERTIGEL 2 Cat No 400000 e MINI TRANSBLOTTER Cat No 430000 e MINI VERTIGEL 2 Cat No 400000 e SEMI DRY BLOTTER Cat No 450000 e PS 305 XL Cat No 170500 e MINI VERTIGEL 2 Cat No 400000 e SEMI DRY BLOTTER Cat No 450000 e MINI VERTIGEL 2 Cat No 400000 e SLIM BLOTTER Cat No 504001 e PS 304 XL Cat No 160600 pational
10. PACK G R f 3310PG COMPREND MAXIGEL THERMO 2 PS 305 XL UN PORTOIR GRATUIT POUR LACHAT DE DEUX PEIGNES SUPPLEMENTAIRES BT 0I AIDES NUCLEIQUES MAXIGEL XL Ref 812325 JUSQU A 500 ECHANTILLONS Avec portoir 23 x 25 cm Ref 8102325 CARACTERISTIQUES Volume de tampon 3 000 ml Dimensions de la cuve Lx p xh 32 x 37 5 x 10 5 cm 23 cm 23 cm 40 cm 25 cm Portoir R f 8112340 Portoir R f 8102325 Base de coulage de gel R f 340600 pour pour 12 peignes pour 10 peignes Portoir 23 x 25 cm R f 8102325 pour MAXIGEL XXL seulement pour MAXIGEL XL et XXL Portoir 23 x 40 cm R f 8112340 MAXIGEL XXL Cat No 812340 JUSQU A 600 ECHANTILLONS Avec portoir 23 x 40 Ref 8112340 Recoit galement Portoir 23 x 25 cm R f 8102325 CARACTERISTIQUES Volume de tampon 4 500 ml Dimensions de la cuve Lx p xh 32 x 53 x 10 5 cm PACK R f 8100PH COMPREND MAXIGEL XXL PS 608 CUVES POUR ACIDES NUCLEIQUES E 8 BLD ES 1 BLD APELEN 4426 Guides pour depot des chantillons Tous les peignes sont compatibles avec les pipettes multicanaux APPLICATION Cuve pour lectrophorese haute r solution et s parations de grandes quantit s d chantillons Analyses des ADN ARN et screening rapide pour contr le des PCR jusqu 5 ou 6 plaques 96 chantillons utilisant le portoir 25 cm ou 40 cm REFERENCES DE COMMANDE 812325 MAXIGE
11. Pas de 1 mA Gamme de Puissance R solution 0 3 300 watts Pas de 1 watt 0 3 300 watts Pas de 1 watt Gamme de temp rature R solution 0 99 C Pas de 1 C 0 99 C Pas de 1 C Int grateur R solution 1 99999 Volt Hr Pas de 1 Volt Heure 1 99999 Volt Hr Pas de 1 Volt Heure Minuterie R solution 1 9999 minutes Pas de 1 minute 1 9999 minutes Pas de 1 minute Valeur minimum r gul e 10 Volt 1 mA 1 watt 10 Volt 1 mA 1 watt Gamme d affichage R solution 0 3000 Volt Pas de 10 Volt 0 1000 Volt Pas de 10 Volts 0 300 mA Pas de 1 mA 0 600 mA Pas de 1 mA 0 300 watts Pas de 1 watt 0 300 watts Pas de 1 watt 0 99 C Pas de 1 C 0 99 C Pas de 1 C 0 99999 Volt Hr Pas de 1 Volt Heure 0 99999 Volt Hr Pas 1 Volt Heure 0 9999 minutes Pas de 1 minute 0 9999 minutes Pas de 1 minute Valeur minimum de d bit 10 Volt 15 uA 0 3 W 10 Volt 15 pA 0 3 W D bit minimum avec le programme 28 10 Volt 0 mA 0 W 10 Volt mA 0 W D tection de fautes stop alarme sonore et messages 5 stop alarme sonore et messages affich s Dimensions l x p x h 27x 34 x 11 cm 27 x34 x 11 cm d PUISSANT FACILE A UTILISER ROBUSTE PS 3003 Ref 178000 3000 Volts e 300 mA 300 Watts e Voltage Constant e Current Constant e Puissance Constante CARACTERISTIQUES Les derniers param tres programm s sont automatiquement m mori
12. paisseur e Voltage de 150 a 300 Volts Fournie avec Compartiment inf rieur couvercle avec cordons e Temps de migration 30 90 minutes d alimentation solidaires module de migration d un double corps pour refroidissement 4 plaques de verre avec encoche 4 plaques de verre pleines 20 x 10 cm 2 jeux d espaceurs Teflon de 0 8 mm d paisseur 2 peignes Teflon 20 dents 0 8 mm d paisseur Volume total de tampon 600 800 ml volume des chantillons 97 ul 2 peignes Teflon 15 dents 0 8 mm Nombre maximum d chantillons 2x25 d paisseur volume des chantillons 144 ul 1 plaque d obturation Dimensions de la cuve P 15 x 28 x 13cm pour migrer 1 gel joints et accessoires de remplacement Base de coulage de gel notice d emploi Peignes et accessoires Pages 94 95 00 CUVES POUR PROTEINES 4 12 M GENERATEURS CONSEILLES Page 62 APELEX 5 304 xL x E APELEX 5 305 xL Ao 99 LE e e e e O e e e e O PS 304 XL R f 160600 PS 305 XL R f 170500 CUVES DE TRANSFERT CONSEILL ES Pages 98 101 Large Transblotter Ref 440000 Slim Blotter R f 504015 Large Semi Dry Blotter Ref 460000 0 PROTEINES MIDI VERTIGEL 2 a a CUVE IDEALE POUR UNE SEPARATION RAPIDE SUR GELS DE MOYENNES DIMENSIONS DESCRIPTION Dimensions utiles des gels 15 x 12 5 cm Lx H e Plaques de verre 16 cm x 14 16 cm Hauteur 480
13. 18 5 Log 0 58 GC 11 8 GC 0 50 96 Formamide 820 1 1ug of L000bp DNA 1 51pmoles TMpna RNA 81 5 16 6 log o Na 0 41 GC 11 8 96GC 0 63 Formamide 600 length avg MW of a base pair 650 Tm decreases by 1 C for every 1 increase in mismatches Tm decreases by 0 5 C for every increase of 1 in formamide Protein Data Ohm s law UV Absorbance of DNA and RNA Average MW of an amino acid 110 daltons grams V IR where Nucleic Acid ug ml to give an 260 1 0 A260 A280 of pure material SDS bound per gram of protein 1 2 average V Voltage Aaen 1mg ml solution 0 4 1 5 range can be as R 2 ds DNA 50 1 8 resistance much as 0 0 2 65 Power IV ss DNA 37 1 8 1 9 RNA 40 37 d Eleclrophorese Francals Pages 58 103 Sommaire Liste des applications sur notre site www apelex fr Guide de s lection cuves et g n rateurs G n rateurs PS 304 XL PS 305 XL PS 503 XL e PS 202 PS 608 PS 1006 PS 9009 TX pour IEF s quencage SSCP G n rateurs programmables PS 9009 TX PS 1006 P e PS 3003 Packs pour Electrophor se des ADN ARN Cuves horizontales pour Electrophor se des ADN ARN e MICROGEL 2 e MINIGEL 2 e MIDIGEL 2 e MIDIGEL XL e MAXIGEL ECO 2 e MAXIGEL THERMO 2 e MAXIGEL XL MAXIGEL XXL Cuves verticales pour lectrophor se des prot ines Syst mes pour SDS et gels pr coul s e MINI VERTIGEL 2 pour 2 gels
14. 400000 e MINI TRANSBLOTTER Ref 430000 e PS 304 XL Ref 160600 e MINI VERTIGEL 2 Ref 400000 e MINI TRANSBLOTTER Ref 430000 APELEX 5 305 xL mum REG O MINI VERTIGEL 2 R f 400000 e TRANSFERT SEMI SEC Ref 450000 e PS 305 XL Ref 170500 e MINI VERTIGEL 2 Ref 400000 e TRANSFERT SEMI SEC R f 450000 e MINI VERTIGEL 2 Ref 400000 e SLIM BLOTTER Ref 504001 e PS 304 XL Ref 160600 ANAMED GELS PRE COULES APELEX ANAMED Les gels ci dessous sont fabriqu s et coul s dans des cassettes a usage unique de 10 x 10 cm qui s adaptent la cuve Mini Vertigel 2 Ref 400000 et Mini Vertigel 2 PC Ref 400800 Les gels sont livr s en packs de 10 cassettes Les gels pr coul s ANAMED sont des gels natifs Toutefois ils sont parfaitement utilisables en version native ou pour des lectrophor ses SDS Tous les gels Laemmli disposent d un gel de chargement de 4 Gels pr coul s APELEX ANAMED Mini Vertigel 2 PC R f 400800 Nos gels subissent un contr le de qualit strict pendant Pour gels pr coul s 10 x 10 cm leur production et sont fournis avec un certificat Module de migration quip d un double corps pour d analyse GLP 115 seront stock s dans votre refroidissement compartiment inf rieur et couvercle r frig rateur et resteront fiables jusqu 6 mois Livr e sans plaques de verre sans peignes ni
15. Compatible avec portoirs 23 x 40 cm et 23 x 25 cm 173000 G n rateur PS 608 paisseur Nombre de dents Volume des chantillons Largeur des dents 810125 Peigne 1 0 mm 16 ul 7 2 mm 810150 Peigne 1 0 mm 7 ul 3 0 mm 8101242 Peigne 1 0 mm 26 24 2 mars 16 7 ul 7 2 3 0 mm 811525 Peigne 1 5 mm 24 ul 7 2 mm 811550 Peigne 1 5 mm 10 ul 3 0 mm 8115242 Peigne 1 5 mm 26 24 2 mars 24 10 ul 7 2 3 0 mm 812025 2 32 ul 7 2 mm pour 1 gel de 5 mm d paisseur CU DOUR PRO GELS POLYACRYLAMIDE VERTICAUX Notre gamme de cuves VERTIGEL combine polyvalence d emploi et s curit d utilisation La qualit d usinage et d assemblage l paisseur des pieces en acrylique la robustesse des mat riaux associ s sa fabrication offrent l utilisateur une garantie de r sultats reproductibles et de long vit Les plaques de verre quipant les cuves VERTIGEL sont coup es et polies avec soin Leur plan it est garantie assurant ainsi l obtention d une paisseur de gel uniforme Les espaceurs et peignes sont en t flon Ils ne pr sentent aucun d faut d usinage ou de d coupe FACILIT D UTILISATION Les plaques de verre sont directement install es sur le module de migration lequel est ins r sur une base horizontal tanche afin de proc der au coulage du gel Une fois le gel polym ris le module de migration est s par de la base de coulage et install simplement dans la cuve de migrati
16. DESCRIPTION 420000 H VERTIGEL 2 Livr e complete avec accessoires pour couler et migrer 2 gels de 1 5 mm d paisseur Fournie avec Compartiment inf rieur couvercle avec cordons d alimentation solidaires module de migration quip d un double corps pour refroidissement 4 plaques de verre avec encoche 4 plaques de verre pleines 20 x 20 cm 2 jeux d espaceurs Teflon de 1 5 mm d paisseur 2 peignes Teflon 15 dents 1 5 mm d paisseur volume des chantillons 271 ul 2 peignes Teflon 20 dents 1 5 mm volume des chantillons 182 pl 1 plaque d obturation pour migrer 1 gel joints et accessoires de remplacement Base de coulage de gel notice d emploi Dimensions utiles des gels 19 x 18 5 cm Lx H e Plaques de verre 20 cm x 18 20 cm Hauteur e Volume d acrylamide pour 2 gels 50 ml e Courant constant 15 75 mA par gel e Voltage de 150 1000 V avec cryostat F12ED e Temps de migration 30 180 minutes CARACTERISTIQUES Volume total de tampon 1 300 ml Nombre Maximum d chantillons 50 Dimensions de la cuve Hx lx P 30 x 24 x 16 cm Peignes et accessoires Pages 94 95 CUVES POUR PROTEINES BH GENERATEURS CONSEILLES Pages 62 64 PS 503 XL Ref 172500 PS 608 Ref 173000 CUVES DE TRANSFERT CONSEILLEES Pages 98 101 Large Transblotter Ref 440000 Slim Blotter Ref 504020 G n rateurs conseill s PS 608 ou PS 202 fy Cryostat F12 ED Large Semi Dry Blotter R f 460000 Be
17. Loading of more than 40 ug lane of protein will cause an upward smearing of the bands TroubleShooting Denaturing Protein Gels Run Conditions The voltage and temperature maintained during the run can have marked effects on the results 1 Electrical parameters Most gels are meant to be run at 5 20V cm Running at too low a voltage too slow allows the bands to diffuse too much before the run 15 finished Running at too high a voltage causes the bands to smear and will lead to overheating which causes smiling 2 Temperature In general gels should be run as cold as is convenient If high voltages are used heat exchange system should be used Overheating of the gel causes changes in the conductivity of the buffer which leads to unpredictable results often distortions of the band pattern or band broadening Diagnosis Gel poorly polymerized A 2 gel overloaded B 3 protease in samples B l or excessive salt in samples 0 1 Run parameters not optimal C L buffer mismatch D U3 4 or fixed charges on gel A 4 Gel heating during run Check run parameters C L buffer 0 3 4 and heat exchange system on gel apparatus C 2 Poor interface between stacking and resolving gels A 3 Poor gel polymerization A 2 Protease degradation or sample oxidation B L or samples not fully denatured B 2 e Ammonium Persulfate or TEMED too old A 2 Too much oxygen in the gel so
18. UP TO 600 SAMPLES With Gel tray 23 x 40 cm Cat No 8112340 Also designed for Gel tray 23 x 25 cm Cat No 8102325 TECHNICAL SPECIFICATIONS Buffer Volume 4 500 ml Unit dimensions wxlxh 32 x 53 x 10 5 cm PACK H Cat No 8100PH INCLUDES MAXIGEL XXL PS 608 TANKS FOR NUCLEIC ACIDS BLD ES Nt 1 BLD APELEX 7575211225 Sample loading guides Multichannel compatible combs APPLICATION Designed for high resolution electrophoresis including DNA analysis PCR screening and long length separation of 25 cm with MAXIGEL XL and 40 cm with MAXIGEL XXL DNA RNA analysis and quick screening of samples from five or six 96 position PCR thermal cycler using 25 cm length gel tray or 40 cm length gel tray ORDERING INFORMATION 812325 MAXIGEL XL Includes 8102325 Removable U V transparent gel tray 23 x 25 cm w x l for up to 10 combs 810125 2 x Combs 25 teeth thickness 1 mm compatible with multichannel pipettes 810150 2 x Combs 50 teeth thickness 1 mm compatible with multichannel pipettes 340600 Gel Casting base with adjustable feet and bubble designed to hold gel tray 23 x 40 cm and 23 x 25 cm 810270 Safety lid with attached power leads 810280 Accessories for buffer circulation 812340 MAXIGEL XXL Includes 8112340 Removable U V transparent gel tray 23 x 40 cm w x l for up to 12 combs 810125 2 x Combs 25 teeth thickness 1 mm compatible with multichannel pipettes 810150 2 x Combs 50 teeth th
19. 0 mm 10 well 10 Tris Gly Gel 1 0 mm 12 well 10 Tris Gly Gel 1 0 mm 15 well 10 Tris Gly Gel 1 5 mm 10 well 10 Tris Gly Gel 1 5 mm 12 well 10 Tris Gly Gel 1 5 mm 15 well IDEAL PERFORMANCE BETWEEN 12 9o Tris Gly Gel 1 0 mm 10 well 12 9o Tris Gly Gel 1 0 mm 12 well 12 9o Tris Gly Gel 1 0 mm 15 well 12 9o Tris Gly Gel 1 5 mm 10 well 12 9o Tris Gly Gel 1 5 mm 12 well 12 9o Tris Gly Gel 1 5 mm 15 well IDEAL PERFORMANCE BETWEEN 14 9o Tris Gly Gel 1 0 mm 10 well 14 9o Tris Gly Gel 1 0 mm 12 well 14 9o Tris Gly Gel 1 0 mm 15 well 14 9o Tris Gly Gel 1 5 mm 10 well 14 9o Tris Gly Gel 1 5 mm 12 well 14 9o Tris Gly Gel 1 5 mm 15 well IDEAL PERFORMANCE BETWEEN 16 Tris Gly Gel 1 0 mm 10 well 16 Tris Gly Gel 1 0 mm 12 well 16 Tris Gly Gel 1 0 mm 15 well 16 Tris Gly Gel 1 5 mm 10 well 16 Tris Gly Gel 1 5 mm 12 well 16 Tris Gly Gel 1 5 mm 15 well IDEAL PERFORMANCE BETWEEN 18 Tris Gly Gel 1 0 mm 10 well 18 Tris Gly Gel 1 0 mm 12 well 18 Tris Gly Gel 1 0 mm 15 well 18 Tris Gly Gel 1 5 mm 10 well 18 Tris Gly Gel 1 5 mm 12 well 18 Tris Gly Gel 1 5 mm 15 well 100 400 kDa 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 60 300 kDa 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 40 250 kDa 10 pieces in a b
20. 0mm 330303 Comb 3 0 mm 30 48 ul 4 0mm 330412 Prep Comb 1 0 mm 1 sample 2 markers 360 uU 2 x 12 ul 90 0 3 mm for a 5 mm thickness gel compatible with multichannel pipettes lh TANKS FOR NUCLEIC ACIDS NUMBER OF COMBS AND GEL DIMENSIONS 20 cm 24 cm 12 cm Double Combs Cat No 334421 and Gel tray Cat No 331100 Gel tray Cat No 331050 Cat No 334422 Both side are compatible with Cooler F12 ED 8 comb slots 2 comb slots multichannel pipettes 21 and 44 teeth SUGGESTED POWER SUPPLIES Page 6 v PS 304 XL e HERO OT CSS w PS 304 XL Cat No 160600 SUGGESTED BLOTTING TANKS Pages 42 43 Mini transblotter Cat No 440000 Slim blotter Cat No 504021 PACK G Cat No 3310PG INCLUDES MAXIGEL THERMO 2 PS 305 XL ONE FREE GEL TRAY WHEN ORDERING 2 ADDITIONAL COMBS awe Cup NUCLEIC ACIDS 7 MAXIGEL XL Cat No 812325 UP TO 500 SAMPLES Designed for Gel tray 23 x 25 cm Cat No 8102325 TECHNICAL SPECIFICATIONS Buffer Volume 3 000 ml Unit dimensions wxlxh 32 x 37 5 x 10 5 cm NUMBER OF COMBS AND GEL DIMENSIONS 23 cm 23 cm 40 cm 25cm Gel tray Cat No 8112340 Gel tray Cat No 8102325 Gel Casting base Cat No 340600 12 comb slots 10 comb slots designed for for MAXIGEL XXL only for MAXIGEL XL and XXL Gel tray 23 x 25 cm Cat No 8102325 Gel tray 23 x 40 cm Cat No 8112340 MAXIGEL XXL Cat No 812340
21. 24 cm l x L avec 2 parois coulissantes pour coulage du gel double peigne 1 0 mm d paisseur 44 21 dents volume des chantillons 14 24 ul R f 334421 compatible pipettes multicanaux couvercle cordons d alimentation accessoires pour refroidissement et circulation de tampon notice d emploi 3310PG PACK G Comprend MAXIGEL THERMO 2 ci dessus R f 331000 et g n rateur PS 305 XL paisseur Nombre de dents Volume des chantillons Largeur des dents 330102 Peigne 2 0 mm 10 128 ul 16 0 mm 330103 SE 3 0 mm 192 ul 16 0 mm 330202 Peigne 2 0 mm 52 ul 5 5mm 330203 Igne 3 0 mm 78 pl 6 5 mm 330302 Peigne 2 0 mm 32 pl 7 0 mm 330303 Peigne 3 0 mm 48 ul 4 0 mm 334421 Double peigne 1 0 mm TT 14 24 pl 3 5 6 mm 334422 Double peigne 2 0 mm 44 21 28 48 ul 3 5 6 mm 330412 Peigne pr paratif 1 0 mm 1 chantillon 1 marqueur 90 0 3 mm pour 1 gel de 5 mm d paisseur compatible avec pipettes multicanaux y CUVES POUR ACIDES NUCLEIQUES B NOMBRE DE PEIGNES ET DIMENSIONS DES GELS 20 cm E ER Double peignes R f 334421 et R f 334422 Portoir R f 331100 Portoir R f 331050 Les 2 c t s 21 et 44 dents sont compatibles Cryostat F12 ED pour 8 peignes pour 2 peignes pipettes multicanaux G N RATEURS CONSEILL S Page 62 I PS 304 XL R f 160600 PS 305 XL R f 170500 CUVES DE TRANSFERT CONSEILL ES Pages 98 99 Mini transblotter Ref 440000 Slim blotter R f 504021
22. 48 ul 6 0 mm 301115 Peigne pr paratif 1 5 mm 1 chantillon 1 marqueur 240 pl 15 ul 40 0 2 5 mm D 340500 Base de coulage de gel avec niveau bulle et pieds r glables pour un gel de 5 mm d paisseur R f 160600 62 Ref 170500 2 6 06 sor 62 MINIGEL TRANSBLOTTER Ref 430000 Page 98 SLIM BLOTTER Ref 504002 Page 99 CUVES POUR ACIDES NUCLEIQUES B MINIGEL 2 Dimensions du gel 10 x 8 cm Lx L Recoit 2 peignes APPLICATION S parations rapides d acides nucl iques RFLP contr le de PCR dun petit nombre d chantillons Convient galement pour Lenseignement e Dimensions r duites La cuve est compl tement transparente aux U V Pas de n cessit de sortir le portoir la cuve complete est plac e sur la table U V CARACTERISTIQUES E ay Volume de tampon 250 ml I p RE p Nombre maximum d chantillons 36 i X Dimensions de la cuve I Lx p x h 19x16x6cm MEME UN PORTOIR GRATUIT POUR L ACHAT DE DEUX PEIGNES SUPPL MENTAIRES REFERENCES DE COMMANDE 311000 MINIGEL 2 Fournie avec Portoir transparent aux U V 10 x 8 cm l x L 2 parois coulissantes peigne 16 dents 1 0 mm d paisseur volume des chantillons 12 pl couvercle paire de cordons d alimentation notice d emploi 3110PA PACK A Comprend MINIGEL 2 ci dessus R f 311000 et g n rateur PS 304 XL paiss
23. 5 mm thick Teflon spacers e Maximum voltage 500 Volt Run time 60 120 minutes 2 1 5 mm 15 wells Teflon combs maximum sample volume 201 ul 2 x 1 5 mm 20 wells Teflon combs maximum sample volume 129 ul 1 blind plate 2 replacement gaskets Gel casting base including 2 silicone seals 2 cams TECHNICAL SPECIFICATIONS 2 x replacement silicone seals instruction manual Total Buffer Volume 600 700 ml Maximum number of samples 48 Range of combs and accessories on page 38 Unit dimensions HxWxD 24x21x14cm Ji TANKS FOR PROTEINS _ 1 SUGGESTED POWER SUPPLIES Pages 6 8 PS 503 XL Cat No 172500 PS 608 Cat No 173000 SUGGESTED BLOTTING TANKS Pages 42 44 Large Transblotter Cat No 440000 Slim Blotter Cat No 504015 Large Semi Dry Blotter Cat No 460000 Jj AN PROTEINS H VERTIGEL 2 DESIGNED FOR LONG LENGTH SEPARATION UP 1000 VOLTS WITH THE COOLER F12 ED DESCRIPTION 420000 2 comes fully equipped for casting and running 2 gels Active gel sizes 19 x 17 5 cm W x H Includes Main buffer tank with safety lid and attached connecting Glass plate sizes 20 W x 18 20 cm L leads 1 upper twin running module equipped by water cooled e Acrylamide capacity 24 49 ml integral cooling chamber 4 x notched glass plate Current constant 15 75 mA gel 4 x plain glass plate 2 sets of 1 5 mm thick Teflon spacers e Maximum voltage 1000 Volt 2 x 20 wells Teflon combs
24. 78 ul 6 5 mm 330302 Comb 2 0 mm 32 ul 4 0 mm 330303 Comb 3 0 mm 48 ul 4 0 mm 334421 Double Sided Comb 1 0 mm m 14 24 pl 3 5 6 mm 334422 Double Sided Comb 2 0 mm 44 21 28 48 ul 3 5 6 mm 330412 Prep Comb 1 0 mm 1 sample 2 markers 360 ul 2 x 12 pl 90 0 3 mm 340500 Separate gel Casting base with adjustable feet and bubble 10 10 for a 5 mm thickness gel compatible with multichannel pipettes TANKS FOR NUCLEIC ACIDS NUMBER OF COMBS AND GEL DIMENSIONS lt mn H Bam mm 20 cm 24cm 12 cm Gel tray Cat 331100 Gel tray Cat No 331050 Double Combs Cat No 334421 and Cat No 334422 8 comb slots 2 comb slots Both side are compatible with multichannel pipettes 21 and 44 teeth SUGGESTED POWER SUPPLIES Page 6 APELEX PS 304 xL e 0 x Volts O SS w PS 304 XL Power Supply Cat 160600 SUGGESTED BLOTTING TANKS Pages 42 43 Mini transblotter Cat No 440000 Slim blotter Cat No 504021 PACK C Cat No 3410PC INCLUDES MAXIGEL ECO 2 PS 305 XL ONE FREE GEL TRAY WHEN ORDERING 2 ADDITIONAL COMBS MEN eise NUcLEIC MAXIGEL THERMO 2 APPLICATION Designed for high resolution electrophoresis including DNA analysis and PCR screening and long length separation of 24 cm DNA RNA analysis and quick screening of all samples from 3 x 96 position PCR thermal cycler on one gel
25. Such gels will take longer than usual to polymerize and will give broad and distorted bands due to local variations gel quality Degassing will remove 02 giving better polymerization but it is not a substitute for fresh reagents Stacking gel This gel sharpens the protein zone before it enters the resolving gel A straight sharp interface 15 critical to good resolution overlay the resolving gel with water saturated n butanol for best results Immobilized charges Acrylamide is subject to oxidation producing acrylic acid The immobilization of acrylic acid in the matrix generates a flow of water electroendosmosis and allows the proteins to Interact with the matrix which leads to band broadening Use only fresh acrylamide solutions within their shelf life which have been properly stored ELECTROPHORESIS TROUBLESHOOTING D Sample Preparation Degradation of samples leads to smeared or doubled bands Overloading will cause bands to smear and will alter band positions 1 Proteolysis Oxidation Proteases are active in the sample buffer keep the Samples cold prior to denaturing and loading Add mercaptoethanol or DIT to the sample buffer if oxidation 15 suspected Proteolysis cause spurious bands or a downward smearing 2 Denaturation Insufficient heating may allow retention of residual secondary structure which will cause bands to migrate at spurious molecular weights and may cause doublets 3 Overloading
26. asa The casting base Walls of the integral cooling Teflon spacers Teflon combs together with the chamber are in ceramic alumina for running module efficient thermal conductivity when allows 1 or 2 gels to connected on the Cooler F12 ED be cast in place THE TWIN AND COOLED VERTIGEL TANKS ARE SUPPLIED FULLY EQUIPPED READY FOR CASTING AND RUNNING 2 GELS D Ji AN PROTEINS MINI VERTIGEL 2 DESCRIPTION 400000 MINI VERTIGEL 2 Comes fully equipped for casting and running 2 gels Also designed for running pre cast gels 10 x 10 cm Includes Main buffer tank with safety lid and attached connecting leads 1 upper twin running module equipped by water cooled integral cooling chamber 2 x notched glass plate 4 x plain glass plate 2 sets of 0 8 mm thick spacers 2 x 0 8 mm 10 wells Teflon combs maximum sample volume 99 ul 2 notched alumina plates 1 blind plate 2 replacement gaskets Gel casting base including 2 silicone seals 2 cams 2 x replacement silicone seals instruction manual 400800 MINI VERTIGEL 2 PC Designed for 2 x pre cast gels 10 x 10 cm Pages 48 52 Supplied with Running Module No other accessories for Casting Gels Range of combs and accessories on page 38 Pre cast gels and reagents i x gt e dee amme deg Active gel sizes 9 x 8 5 cm W x Glass plate sizes 10 cm W x 8 10 cm L e Acrylamide capacity 6 15 ml Current constant 1
27. d paisseur Pas de pieces moul es ou inject es donc pas de d formations d finitives en cas de coulage d un gel trop chaud ou de surchauffe pendant la migration QUALIT Une large gamme de peignes en DELRIN usin e avec pr cision et en une seule pi ce est disponible Les peignes sont sans limitation de dur e de garantie et chang s gratuitement en cas de d t rioration ou de casse Ces peignes existent en diff rentes configurations incluant la compatibilit avec Les pipettes multicanaux PRATIQUE Portoirs transparents aux U V permet tant grace au Bromure d Ethidium la visualisation des bandes Une r gle fluorescente est imprim e sur le cote CUVES POUR ACIDES NUCLEIQUES B Impossibilite d ouvrir le couvercle lorsque les cordons d alimentation sont branch s Support du plateau en Lexan 4 pieds r glables et niveau bulle blanc avec bandes rouges La MINIGEL 2 et la MICROGEL 2 pour meilleure visualisation sont quip es de 4 pieds pendant le d p ts des caoutchouc simples chantillons Impossibilit de brancher les cordons d alimentation lorsque le couvercle est ouvert Connecteurs en m tal dor vitant la corrosion Electrodes longitudinales en fil de platine de 0 3 mm de diam tre assurant un champs lectrique parfait F b LZ as A ul i em mm sg Ww P e dd 2 Les portoirs sont usines de facon a recevoir 1 ou plusieurs pe
28. des gels preparatifs ou analytiques sur gels de 20 x 24 cm Convient egalement pour la separation des ADN et ARN RFLP Screening des PCR sur 3 x 96 chantillons avec 7 peignes Ref 334421 compatibles pipettes multicanaux Longueur de s paration 12 cm ou 24 cm selon Le mod le de portoir choisi e Nombre maximum d chantillons 392 avec 8 peignes Ref 334421 ou R f 334422 compatibles pipettes multicanaux e Portoir 20 x 24 cm recevant 8 peignes e Portoir 20 x 12 cm recevant 2 peignes Peignes 10 20 30 dents et modeles UN PORTOIR GRATUIT POUR L ACHAT DE DEUX PEIGNES SUPPL MENTAIRES pr paratifs Double peignes 44 21 dents compatibles avec pipettes multicanaux e 4 pieds r glables et niveau bulle e Bandes rouges pour meilleure CARACTERISTIQUES visualisation pendant le d p t des Volume de tampon 1 800 ml chantillons Dimensions de la cuve e Ports pour circulation de tampon Lx p xh 28 x 37 x cm REFERENCES DE COMMANDE 341000 MAXIGEL ECO 2 Fournie avec portoir transparent aux U V 20 x 24 cm Lx L avec 2 parois coulissantes pour coulage du gel double peigne 1 0 mm d paisseur 44 21 dents volume des chantillons 14 24 ul R f 334421 compatible pipettes multicanaux couvercle cordons d alimentation accessoires pour circulation de tampon notice d emploi 3410PC PACK C Comprend MAXIGEL ECO 2 ci dessus R f 341000 et g n rateur PS 305 XL paisseur Nombre de dents Volum
29. espaceurs sans base de coulage GELS PR COUL S ANAMED ET R ACTIFS PAGES 48 A 51 Ov erall Performance P E dSs TA v 3 Exec e Date Nase a EN ss 4 lS ls mm RE lt v pr me Basic Acidic Polar EI Nonpolar hydrophobic 57 Modification 5 Sumo M Methyl P Phospho U Ubiquitin nM N Methyl oG O glycosyl nG N glycosyl ppe oujwe Phenylalanine d OH Phe HaC Leucine Leu HC OH Alanine S He on valine CHa Val Tryptophan Leucine i Leu HG EN OH Proline H i Pro Isoleucine lle Methionine Met How to Use This Chart This chart will enable you to identify an amino acid from a single codon Begin in the center with the first nucleotide of the codon and work your way out For example to translate CAU use the Www nationaldiagnostics com first position nucleotide C to identify the correct quadrant of the center circle Next find the second position nucleotide in the first ring surrounding the center A which is at five o clock Finally locate the third nucleotide U in the second ring from the center five o clock Follow the segment outward to the next ring where you will find the single letter code for the am
30. jueinog MODELES COMPACTS LEGERS ET PUISSANTS PS 304 XL Ref 160600 300 Volts e 400 mA PS 503 XL Ref 172500 500 Volts e 300 mA Minuterie et GEL SAVER APELEX PS 304 XL 0 906 CN 70 0 0 9 20 APELEX be 305 e H 6 0 0 0 2 H z PS 305 XL Ref 170500 300 Volts 500 mA Minuterie et GEL SAVER PS 304 XL Gamme de tension R solution 1 300 V Pas de 1 volt Gamme de courant R solution 1 400 mA Pas de 1 mA Puissance maximum 100 W Valeurs minimum affich es 7 SPECIFICATIONS Seuils minimum de r gulation D tection de fautes Temp rature fonctionnelles 1 volt 1 mA 1 volt 15 uA 0 3 W stop alarme sonore message 0 C 40 C GEL GEL SAVER SAVER PS 503 XL PS 305 XL 1 500 V Pas de 1 volt 1 300 V Pas de 1 volt 1 300 mA Pas de 1 mA 1 500 mA Pas de 1 mA 0 999 min Pas de 1 minute 0 999 min Pas de 1 min 100 W 100 W 1 volt 1 mA 1 volt 1 mA 1 volt 15 uA 0 3 W 1 volt 15 pA 0 3 W stop alarme sonore message stop alarme sonore message Dimensions lx p x h Poids 1 8 kg d 18 21 x 8 cm 0 C 40 C 0gc 40 18 x 21 x 8 cm 18 x 21 x 8 cm 1 8 kg 1 8 kg GENERATEURS Ps 503 XL amp PS 305 XL Hi FONCTION DU GEL SAVER EVITER LA DIFFUSION DES BANDES APR S COUPURE DU DEBIT PAR LA MINUTER
31. lagnostics www nationaldiagnostics com PRE MIXED GELS amp SOLUTIONS Acrylamide Native Denatured MBA DNA RNA DNA RNA Protein Ratio bp bp kd NEM 400 1500 70 500 100 200 40 500 20 200 0 400 10 3060 50 300 20 200 GEL MATRICES ORDERING INFORMATION EC 890 200 ProtoGel 37 5 1 200 ml EC 890 450 ProtoGel 37 5 1 450 ml EC 890 1000 ProtoGel 37 5 1 1000 ml 3096 Stabilized 37 5 1 Premixed solution of acrylamid bisacrylamide Ideal for the separation of proteins and polypeptides EC 852 450 AccuGel 29 1 450 ml EC 852 1000 AccuGel 29 1 1 Liter Ready to use monomer Premixed 4096 Acrylamide Bisacrylamide solution for the preparation of electrophoresis gels for Protein separation EC 755 ProtoPrep II Kit 450 ml EC 757 ProtoPrep 11 buffer 200 ml EC 756 ProtoPrep ll concentrate 200 ml EC 758 ProtoPrep II disolution reagent 50 ml Meltable acrylamide matrix for the electrophoretic separation and purification and recovery of proteins After the gel is run proteins from 2 to 200kD can be located excised and recovered from the matrix by melting the gel section in three volumes of disolution reagent The ProtoPrep II will melt only once and remains liquefied EC 810 1000 AcrylaGel 1 Liter AcrylaGel is a 30 stabilized ready to use acrylamide solution EC 820 1000 Bis AcrylaGel 1 Liter Bis AcrylaGel is a ready to use 2 solution of methylene bisacrylamide Also designed for DNA amp RNA separation EC 852 1000 Accu
32. maximum sample volume 182 ul e Run time 30 180 minutes 2 x 15 wells Teflon combs maximum sample volume 271 ul 1 blind plate 2 replacement gaskets Gel casting base including 2 silicone seals 2 cams TECHNICAL SPECIFICATIONS 2 x replacement silicone seals instruction manual Total Buffer Volume 1 300 ml Maximum number of samples 50 Range of combs and accessories on page 39 Unit dimensions HxWxD 30x24x16cm 20 TANKS FOR PROTEINS SUGGESTED POWER SUPPLIES Pages 6 8 5 503 ad PS 503 XL Cat No 172500 PS 608 Cat No 173000 SUGGESTED BLOTTING TANKS Pages 42 44 Large Transblotter Cat No 440000 Slim Blotter Cat No 504020 Suggested with Power Supply PS 608 or PS 202 for Fast Blotting Cooler F12 ED Large Semi Dry Blotter Cat No 460000 J Jo Oh PROTEINS 400000 400615 400815 400915 401015 401215 40SP15 400608 400808 400908 401008 401208 40SP08 401010 401016 401020 401025 400010 400012 401035 400020 400021 401030 480000 481015 481515 482015 482415 4815PREP 48SP15 481008 481508 482008 482408 48SP08 481614 481615 481025 480010 480012 481035 480020 480021 481030 VERTIGEL 2 MINI VERTIGEL 2 fully equipped for 2 x 0 8 mm gel 10 x 10 cm and casting base Includes 2 notched glass plates 4 plain glass plates 2 alumina plates 1 blind plate 2 x 10 wells 0 8 mm combs 4 spacers 0 8 mm 2 replacement gasket internal
33. pieces in a box 20 300 bases 10 pieces in a box 10 pieces in a box 10 pieces in a box il ILLE HI I LE HI UM uM AN 90100 AN 90105 AN 91110 AN 91112 AN 91115 TG 05001 TG 10001 TG 50001 TG 02002 TG 05002 TG 05003 TG 10003 TG 02004 TG 05004 TG 05005 TG 10005 TR 05006 TR 10006 TR 50006 TR 02007 TR 05007 TG 05005 TG 10005 TB 10060 TB 50060 TB 01070 TB 05070 TB 01075 TU 01080 TU 02085 MS 10001 MS 10005 GF 10002 GF 10003 GF 10010 AN 10100 AN 10200 AN 10110 AN 10210 AN 10120 AN 10220 AR 10050 AR 10100 AR 10500 2 ANAMED EMPTY CASSETTES AND COMBS Empty Cassettes 10 x 10cm 1 0 mm 25 pieces Empty Cassettes 10 x 10cm 1 5 mm 25 pieces Combs 10 well 1 0 mm 25 pieces Combs 12 well 1 0 mm 25 pieces Combs 15 well 1 0 mm 25 pieces Buffer Concentrates for Electrophoresis Tris Glycine Applications Tris Glycine SDS Running Buffer 10 x Concentrate Tris Glycine SDS Running Buffer 10 x Concentrate Tris Glycine SDS Running Buffer 10 x Concentrate Tris Glycine SDS Sample Buffer 2 x Concentrate Tris Glycine SDS Sample Buffer 2 x Concentrate Tris Glycine Native Running Buffer 10 x Concentrate Tris Glycine Native Running Buffer 10 x Concentrate Tris Glycine Native Sample Buffer 2 x Concentrate Tris Glycine Native Sample Buffer 2 x Concentrate Transferbuffer for ProGel ProGel P 25 x Concentrate Transferbuffer for ProGel Pr
34. pipettes multicanaux volume des chantillons 24 ul portoir transparent aux 15 x 17 cm Lx L avec 2 parois amovibles pour coulage du gel portoir transparent aux U V 15 x 7 cm 1 L avec 2 parois amovibles pour coulage du gel couvercle cordons d alimentation accessoires pour circulation de tampon notice d emploi 371696 PACK 96S Comprend MIDIGEL XL ci dessus R f 371000 et g n rateur PS 304 XL 371602 Peigne 2 0 mm 16 48 ul 6 0 mm 372703 Peigne 3 0 mm 27 48 ul 4 0 mm 37P112 Peigne pr paratif 2 0 mm 1 chantillon 1 marqueur 280 ul 48 ul 138 5 mm 340500 Base de coulage de gel avec niveau a bulle et pieds r glables pour 1 gel de 5 mm d paisseur compatible avec pipettes multicanaux CUVES POUR ACIDES NUCLEIQUES E 8 NOMBRE DE PEIGNES ET DIMENSIONS DES GELS 15 cm 15cm 15 cm 12 cm 7 cm Portoir R f 371520 Portoir R f 371517 Portoir R f 371512 Portoir R f 371507 pour 8 peignes pour peignes pour 4 peignes pour 2 peignes G N RATEURS RECOMMAND S Page 62 APELEX 5 3051 Bob Kam 279 am RS D O PS 304 XL Ref 160600 PS 305 XL Ref 170500 CUVES DE TRANSFERT CONSEILLEES Pages 98 99 Mini transblotter Ref 440000 Slim blotter R f 504017 PACK 965 R f 371696 COMPREND MIDIGEL XL pour 96 chantillons PS 304 XL EN AIDES NUCLEIQUES 6 MAXIGEL 2 APPLICATION Cuve recommand e pour
35. running module with internal cooling chamber safety lid with power cords gel casting base user s manual 6 teeth comb 1 5 mm Well volume 266 ul Teeth wide 11 mm 8 teeth comb 1 5 mm Well volume 42 ul Teeth wide 7 7 mm 9 teeth comb 1 5 mm multichannel pipette Well volume 138 pl Teeth wide 138 mm 10 teeth comb 1 5 mm Well volume 134 ul Teeth wide 5 7 mm 12 teeth comb 1 5 mm Well volume 103 ul Teeth wide 4 3 mm Set of 2 x 1 5 mm spacers 6 teeth comb 0 8 mm Well volume 142 ul Teeth wide 11 mm 8 teeth comb 0 8 mm Well volume 99 ul Teeth wide 7 7 mm 9 teeth comb 0 8 mm multichannel pipette compatible Well volume 41 pl Teeth wide 138 mm 10 teeth comb 0 8 mm Well volume 73 ul Teeth wide 5 7 mm 12 teeth comb 0 8 mm Well volume 55 yl Teeth wide 4 3 mm Set of 2x 0 8 mm spacers Plain glass plate 10 x 10 cm 1 Notched glass plate 10 x 10 cm 1 piece Notched alumina plate 10 x 10 cm 1 piece Blocking plate for running one gel Blind plate Water cooled removable Running module already included in the package Cat No 400000 Replacement clamps for running module Cat No 400010 x 2 Replacement gasket for running module Cat No 400010 x 2 Gel casting base already included in the package Cat No 400000 Replacement gasket for casting base x 2 Gel casting base running module MIDI VERTIGEL 2 fully equipped for 2 x 1 5 mm gel 16 x 14 cm W x H casting base Includes 4 notche
36. the buffer solution VarioGel is not intended to substitute by all means the performance of a pH alkaline Tris Glycine or Tricine gel We know by fact certain applications which run much better in terms of separation in weak alkaline buffer medias MIGRATION PATTERN OF PROTEMIX ON VARIOGEL 9 AND 4 12 220 kDa R 9 VG Tricine 9 VG Mops 4 12 VG Tricine 4 12 VG Mops The best feature of 0 000 VarioGel 116 kDa 0 100 97 0 kDa e Possible variations in 0 200 buffer solutions to be 66 0 kDa used for selection of 0 300 55 6 kDa the best resolution in assis 9 molecular sizes a user wants to look at 290kpa 959 e Shorter runtime down to 35 minutes instead 140 0 970 of 90 minutes 6 1 kDa 0 800 210kpa 9609 e No need of refrigerator e34kDa 5 storage 2 5 kDa 1 000 MIGRATION PATTERN OF DNA FRAGMENTS ON VARIOGEL 9 AND 4 12 0 R 9 VG Tricine 9 VG Mops 4 12 VG Tricine 4 12 VG Mops 2645 bp 0 000 1605 bp 1198 bp 909 ES Guaranteed shelf life 676bp 0200 least 6 months 517 after production date P 0 300 460 b E I e Very attractive In price 296 bp 09400 ES QC certificates 3505 0500 available on request 222 bp 7 0 600 e Saving lab 179 bp aving laboratory costs 126 bp 970
37. the transfer membrane e Replaceable platinum electrodes e Safety lid provides complete protection to the user e Western Blot e Southern Blot e Northern Blot ORDERING INFORMATION SUGGESTED POWER SUPPLY 430000 Electro blotting unit for up to four 10 x 10 cm gels Page d Includes 4 compression cassettes 8 x fibre packs ACCESSORIES for unit Cat No 430000 431010 Compression cassette 431PAD Fibre pads 10 x 10 cm PK 4 TECHNICAL SPECIFICATIONS Buffer Volume 1300 ml Unit dimensions x W x D 15x 13x 15 cm Transfer Area 9 cm W x 9 cm L Run Time 4 hours Overnight PS 305 XL Cat No 170500 LARGE GEL TRANSBLOTTER FOR UP 10 2 GELS 20 x 20 cm SUGGESTED POWER SUPPLY Page 8 ORDERING INFORMATION PS 202 Cat No 176000 or PS 608 Cat No 173000 Includes 2 compression cassettes 4 x fibre packs ACCESSORIES for unit Cat No 440000 Buffer Volume 3000 ml Unit dimensions H x W x D 25 x 23x 10 cm 441010 Compression cassette 20 x 20 cm with pads TE Deer res ae Teall 440PAD Fibre pads 20 x 20 cm PK 4 Run Time 4 hours Overnight TANKS FOR BLOTTING THE SLIM FAMILY FOR FAST BLOTTING Designed for all blottings PROTEINS WESTERN with standard alloy anode and cathode DNA SOUTHERN with platinized Titanium anode RNA NORTHERN with platinized Titanium anode e Western Blots of 0 75 or 0 8 mm thick gels are cleared of proteins up to 170kD in size in 40 minutes at 24 Volts e Western Blots o
38. then the START mode turns on automatically at the preset values Recommended for DNA RNA sequencing and agarose electrophoresis available for Protein electrophoresis PS 3003 d Electrophoresis Power Supply 6 6 6 DANGER HIGH VOLTAGE Three clear displays for reading the actual and preset voltage current and power Three safety outlets This microprocessor controlled power supply is equipped with a circuit breaker which automatically cuts the electrical supply in case of ground leakage detection 500 pA short circuit open circuit load connected or overload A yellow blinking LED and an audible safety alarm ringing for 10 seconds indicate the FAULT situation SPECIFICATIONS 10 3000 volts 10 volts 15 00 1 0 3 300 watts 1 watt Maximum power 300 watts Minimum regulated value 10 volts 1mA 1 watt 0 3000 volts 10 volts 0 300mA 1mA 0 300 watts 1 watt Minimum output 10 volts 15pA 0 3 W Fault detection stop and audible alarm Operating temperature 0 C 40 C 27 x 34x 11 cm Output range resolution Value display range Dimensions w x d x h Weight 4 kg POWER SUPPLIES COST EFFECTIVE SOLUTIONS WITH OUR DNA ELECTROPHORESIS PACKS MAXIGEL ECO 2 PS 305 XL MIDIGEL 2 PS 304 XL MAXIGEL THERMO 2 PS 305XL 9 9 MAXIGEL XXL REFERENCES PACK A Cat 3120 Tank MINIGEL 2 Cat No 311000 Power Supply PS 304
39. well 8 16 Tris Gly Gel 1 0 mm 12 well 8 16 Tris Gly Gel 1 0 mm 15 well 8 16 Tris Gly Gel 1 5 mm 10 well 8 16 Tris Gly Gel 1 5 mm 12 well 8 16 Tris Gly Gel 1 5 mm 15 well IDEAL PERFORMANCE BETWEEN 4 20 Tris Gly Gel 1 0 mm 10 well 4 20 Tris Gly Gel 1 0 mm 12 well 4 20 Tris Gly Gel 1 0 mm 15 well 4 20 Tris Gly Gel 1 5 mm 10 well 4 20 Tris Gly Gel 1 5 mm 12 well 4 20 Tris Gly Gel 1 5 mm 15 well IDEAL PERFORMANCE BETWEEN 10 20 Tris Gly Gel 1 0 mm 10 well 10 20 Tris Gly Gel 1 0 mm 12 well 10 20 Tris Gly Gel 1 0 mm 15 well 10 20 Tris Gly Gel 1 5 mm 10 well 10 20 Tris Gly Gel 1 5 mm 12 well 10 20 Tris Gly Gel 1 5 mm 15 well ANAMED PROGEL IRIS GLYCINE GRADIENT GELS 30 300 kDa 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 200 kDa 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 5 200 kDa 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 5 100 kDa 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box PROGEL P TRIS TRICINE GELS SCHAEGGER amp VON JAGOW GELS PREFERABLY DESIGNATED FOR PEPTIDE ELECTROPHORESIS IDEAL
40. 0 4 mm Peigne 15 dents 0 8 mm volume des puits 107 pl Largeur des dents 6 1 mm Peigne 20 dents 0 8 mm volume des puits 69 ul Largeur des dents 3 9 mm Peigne 24 dents 0 8 mm volume des puits 51 ul Largeur des dents 2 9 mm Espaceurs 0 8 mm x 2 Plaque de verre pleine 16 x 14 cm x 1 Plaque de verre avec encoche 16 x 14 cm x 1 Paroi d obturation pour migration d un seul gel Module de s paration seul avec lectrodes fourni avec la cuve R f 480000 Pinces de rechange pour module R f 480010 x 2 Joint de rechange pour module R f 480010 x 2 Base de coulage de gel d j inclus dans la r f rence R f 480000 Base de coulage de gel module de s paration CUVES POUR PROTEINES VERTIGEL 2 410000 411015 411515 412015 412515 41SP15 411008 411508 412008 412508 41SP08 412010 412012 411025 410010 410012 411035 410020 410021 411030 420000 421015 421515 422015 422515 4215PREP 42SP15 421008 421508 422008 422508 42SP08 422020 422022 421025 420010 420012 421035 420020 420021 421030 Mini Wide Vertigel 2 pour 2 gels 19 x 8 5 cm L x paisseur 0 8 mm plaques de verre 20 x 10 cm L x H avec base de coulage de gels et joints de remplacement 4 plaques de verre avec encoche 4 plaques de verre pleines 20 x 10 cm 1 paroi d obturation 2 peignes 15 dents 0 8 mm 2 peignes 20 dents 0 8 mm 8 espaceurs 0 8 mm couvercle avec cordons solidaires 2 joints de remplace
41. 000 MIDI VERTIGEL 2 Volume acrylamide pour 2 gels 27 ml Livr e complete avec accessoires pour couler et migrer 2 gels e Courant constant 15 a 50 mA par gel de 1 5 mm d paisseur e Voltage de 60 120 Volts Fournie avec Compartiment inf rieur couvercle avec cordons e Temps de migration 30 90 minutes d alimentation solidaires module de migration quip d un double corps pour refroidissement 4 plaques de verre avec encoche 4 plaques de verre pleines 16 x 14 cm 2 jeux d espaceurs Teflon de 1 5 mm d paisseur 2 peignes Teflon 15 dents 1 5 mm Volume total de tampon 600 700 ml d paisseur volume des chantillons 201 ul 2 peignes 20 dents Nombre Maximum d chantillons 48 1 5 mm d passeur volume des chantillons 129 ul 1 plaque Dimensions de la cuve H x Ux P 24 x 21 x 14 cm d obturation pour migrer 1 gel joints et accessoires de remplacement Base de coulage de gel notice d emploi Peignes et accessoires Pages 94 95 d CUVES POUR PROTEINES 4 2 M G N RATEURS CONSEILL S Page 62 _ APELEX 503 xL 29 X 0 0 0 0 PS 503 XL R f 172500 PS 305 XL R f 170500 CUVES DE TRANSFERT CONSEILL ES Pages 98 101 Large Transblotter R f 440000 Slim Blotter R f 504015 Large Semi Dry Blotter R f 460000 d H VERTIGEL 2 PROTEINES ES CUVE CONCUE POUR DES SEPARATIONS NECESSITANT JUSQU 1 000 VOLTS AVEC CONNEXION DU CRYOSIAT F12 ED
42. 1 for 20 x 20 cm Gels 1 Drying frame 100 Cellophane sheets and 500 ml AnaRapid AnaFix Drying frame for one piece of a 10 x 10 cm Gel 1 Pack 2 frames AnaFix Drying frame II for a 20 x 20 cm Gel or 4 anamed gels Cellophane sheets precut 1 Pack 200 pieces Cellophane sheets II precut 1 Pack 100 pieces AnaRapid Geldrying solution AnaRapid Geldrying solution AnaRapid Geldrying solution for 10 x 10 cm gels for 20 x 20 cm gels 1 Pack 1 Pack 1 Pack 1 Pack 1 Pack 500 ml 1000 ml 5000 ml 20 ml 50 ml 500 ml 1000 ml 20 ml 50 ml 500 ml 1000 ml 500 ml 1000 ml 5000 ml 20 ml 50 ml 500 ml 1000 ml 1000 ml 5000 ml 10 ml 50 ml 10 ml 10 ml 20 ml 1 ml 1 ml 1 Pack 1000 ml 1 Pack 1 Pack 1 Pack 1 Pack 1 Piece 1 Pack 1 Pack 500 ml 1000 ml 5000 ml A d ational lagnostics www nationaldiagnostics com D Matrix Related Problems Problems with the matrix will manifest as smeared bands and or loss of resolution Note that most total failures of electrophoresis are not matrix related see D Buffer related problems 1 Pore size This is determined by the amount of matrix and cross linker used suggested ranges are given on the web site Use of the wrong formulation will result the region of interest running off of the gel or failing to enter the gel sufficiently to resolve Larger pore sizes also allow more diffusion resulting i
43. 10 x 10 cm e MINI WIDE VERTIGEL pour 2 gels 20 x 10 cm e MIDI VERTIGEL 2 pour 2 gels 16 x 18 cm e H VERTIGEL 2 pour 2 gels 20 x 20 cm e Accessoires pour cuves VERTIGEL e Cuve conomique MaeGeleln Cuves pour Blotting lectro transfert e Cuves pour Blotting en milieu liquide e Cuves pour Blotting semi sec e Comment choisir sa cuve de Blotting Packs pour lectrophor se des prot ines et Blotting Gels pr coul s ANAMED et r actifs Gels en solution Gels pr coul s Tampons et solutions Guide de dysfonctionnement Informations pratiques pour l lectrophor se Poster des codes g n tiques 61 62 69 62 63 64 65 66 67 66 67 68 69 70 83 72 73 74 76 78 80 82 84 85 86 88 90 92 94 95 96 97 98 101 98 99 100 101 102 103 47 48 52 93 04 96 27 104 d 9 e 222 285 5000 996 2 KAN mg e e 5 geg 26900 _ 900000 canat 308 QUALITE Chaque g n rateur est test selon une proc dure testant et confirmant sa capacit d biter sa pleine puissance sur de longues PERFORMANCE Les g n rateurs pour lectrophor se APELEX sont fabriqu s en France Ils sont quip s de composants de haute qualit Ils ont ete d velopp s en respectant dur es des crit res de performance S CURIT Avant emballage un contr le et de long vit Les g n rateurs APELE
44. 2 PS 304 XL UN PORTOIR GRATUIT POUR LACHAT DE DEUX PEIGNES SUPPLEMENTAIRES CUVES POUR ACIDES NUCLEIQUES _ It MIDIGEL XL APPLICATION Cuve recommand e pour des gels preparatifs ou analytiques sur gels de tailles moyennes Convient egalement pour la separation des ADN et ARN RFLP Screening des PCR sur 96 echantillons e Un choix de 4 longueurs de gels e Portoir 15 x 20 cm pour 8 peignes e Portoir 15 x 17 cm pour 6 peignes e Portoir 15 x 12 cm pour 4 peignes Portoir 15 x 7 cm pour 2 peignes Peignes conventionnels pr paratifs peignes 16 et 32 dents compatibles pour pipettes multicanaux e 4 pieds r glables et niveau bulle Bandes rouges pour meilleure UN PORTOIR GRATUIT POUR L ACHAT DE DEUX PEIGNES SUPPL MENTAIRES visualisation pendant le d p t des chantillons CARACTERISTIQUES e Ports pour circulation de tampon Volume de tampon 1 200 ml Dimensions de la cuve Lx p xh 30 x 20 x 8 cm REFERENCES DE COMMANDE 370000 MIDIGEL XL Fournie avec Portoir transparent aux U V 15 x 17 cm 1 x L avec 2 parois amovibles pour coulage du gel peigne 27 dents 2 0 mm d paisseur volume des chantillons 32 1 couvercle cordons d alimentation accessoires pour circulation de tampon notice d emploi 3700PB PACK B Comprend MIDIGEL XL ci dessus R f 370000 et g n rateur PS 304 XL 371000 MIDIGEL XL pour 96 chantillons Fournie avec 6 peignes 16 dents 1 0 mm R f 371601 compatibles avec
45. 21000 MIDIGEL 2 Includes Removable U V transparent gel tray 10 x 14 cm w x l 2 removable casting gates 2 0 mm thickness 16 teeth comb sample volume 24 ul safety lid set of power leads buffer circulation accessories user s manual 3210PF PACK F Includes MIDIGEL 2 Fully equipped as above Cat No 321000 Power supply PS 304 XL Thickness Teeth Sample volume Well width 320103 Comb 3 0 mm 10 72 ul 6 0 mm 16 320163 Comb 3 0 mm 36 ul 3 0 mm 320181 Comb 1 0 mm 18 14 ul 3 5mm compatible with multichannel pipettes 320121 Prep Comb 1 0 mm 1 sample 2 markers 360 ul 2 x 12 ul 90 0 3 mm 340500 Separate gel Casting base with adjustable feet and bubble for a 5 mm thickness gel TANKS FOR NUCLEIC ACIDS NUMBER OF COMBS AND GEL DIMENSIONS 10 cm iu SJ e 4 LS lt 14 8 24 Gel tray Cat No 321100 Gel tray Cat No 321050 Delrin Combs 4 comb slots 2 comb slots SUGGESTED POWER SUPPLIES Page 6 APELEX PS 304 XL e m 6 a EUM 1 O SS w PS 304 XL Cat No 160600 PS 305 XL Cat No 170500 P SUGGESTED BLOTTING TANKS Pages 42 43 Mini Transblotter Cat No 430000 Slim blotter Cat No 504001 PACK Cat No 3210PF INCLUDES MIDIGEL 2 PS 304 XL ONE FREE GEL TRAY WHEN ORDERING 2 ADDITIONAL COMBS PS 304 XL awe NUCLEIC ACIDS 7 MIDIGEL XL APPLICATION Accomodates standard gels fo
46. 412515 411815 413615 41SP15 411008 411508 412008 412508 411808 413608 41SP08 412010 412012 410010 410012 411035 410020 410021 411030 420000 421015 421515 422015 422515 4215PREP 42SP15 421008 421508 422008 422508 42SP08 422020 422022 420010 420012 421035 420020 420021 421030 MINI WIDE VERTIGEL 2 fully equipped for 2 x 0 8 mm gel 20 x 10 cm W x H gel casting base Includes 4 notched glass plates 4 plain glass plates 1 blind plate 2 x 15 wells 0 8 mm combs 2 x 20 wells 0 8 mm combs 4 spacers 0 8 mm 2 replacement gasket internal running module with internal cooling chamber safety lid with power cords gel casting base user s manual 10 teeth comb 1 5 mm Well volume 449 ul Teeth width 13 mm 15 teeth comb 1 5 mm Well volume 271 pl Teeth width 8 mm 20 teeth comb 1 5 mm Well volume 182 ul Teeth width 5 5 mm 25 teeth comb 1 5 mm Well volume 129 pl Teeth width 4 mm 18 teeth comb 1 5 mm MC pipette compatible Well volume 156 pl Teeth width 6 5 mm 36 teeth comb 1 5 mm MC pipette compatible Well volume 64 ul Teeth width 2 7 mm Set of 2 x 1 5 mm spacers 10 teeth comb 0 8 mm Well volume 239 ul Teeth width 13 mm 15 teeth comb 0 8 mm Well volume 144 ul Teeth width 8 mm 20 teeth comb 0 8 mm Well volume 97 ul Teeth width 5 5 mm 25 teeth comb 0 8 mm Well volume 69 ul Teeth width 4 mm 18 teeth comb 0 8 mm MC pipette compatible Well volume 78 ul Teeth widt
47. 5 30 mA gel e Maximum voltage 300 Volt e Run time 30 60 minutes TECHNICAL SPECIFICATIONS Total Buffer Volume 300 400 ml Maximum number of samples 2x15 Unit dimensions HxWxD 14 x 17 x 12 4 UM MINI VERTIGEL 2 PC Cat No 400800 Designed for precast gels 10 x 10 cm only Pages 48 52 TANKS FOR PROTEINS SUGGESTED POWER SUPPLIES Page 6 APELEX m L e Aum o e Een lt PS 304 XL Cat No 160600 PS 305 XL Cat No 170500 SUGGESTED BLOTTING TANKS Pages 42 44 1 Mini Transblotter Cat No 430000 2 Slim Blotter Cat No 504001 3 Mini Semi Dry Blotter Cat No 450000 PACK See more packs page 46 _ Psw e O 2 Cat 400000 MINI TRANSBLOTTER Cat 430000 e PS 304 XL Cat No 160600 e MINI VERTIGEL 2 Cat No 400000 e MINI TRANSBLOTTEr Cat No 430000 AN PROTEINS MINI WIDE VERTIGEL 2 RUN UP TO 50 SAMPLES DESCRIPTION 410000 MINI WIDE VERTIGEL 2 comes fully equipped for casting and running 2 gels Active gel sizes 19 x 8 5 cm W x Includes Main buffer tank with safety lid and attached connecting Glass plate sizes 20 cm W x 8 10 cm L leads 1 upper twin running module equipped by water cooled e Acrylamide capacity 15 30 ml integral cooling chamber 4 x notched glass plate Current const
48. 5B 5B 5 eoeeeeeeeeeseeeeeeees Patate eo 0 0 0 0 0 0 0 0 De cee 8 De oo 00 0 OB 0 O O 5 n n s x Oo 00 060 8 0 0 4 5 R ee e e O 0 o o o o o e Te 0 0 ewe e e e ow 00 0 0 0 O 5 5 Ze LV FFA o ot ot Ba fa e H ooo 0005 5 55 e m Ww Xx ee 00 00 506 5 6 9 9 geg e Ve 0 0 0 0 a D K D 24 a EEK rete eo 0 eee 0 e o 5 6 i e e e o e s gr sg sg RR S 0700070070009 e WWW os Fuka a o o o 0 0 o Ze 2 e s E one we e 200600004000 KEANE Sete 0 0 00 0 H e e 5 e oe 000 0 5 5 5 9 ee s BW E RR 000 0 0 0 0 O O 9 9 9 9 m 000000 06 O O 9 0 9 5 s s k ow 0 0 06 00 OB 5 5B 9 9 te eo 000 68 O 5 9 9 b b 90 0 0 0 0 O DO 5 9 5 9 a e eeeeeeeeeeeeeeeeeees ee ee eeeeeeeeeeeeege e a 600000000040 200000000000 oo 0 00 00 O O9 0 9 9 000000000000 0 e e n se e s ooo0000 0 00 00 O0 O 0 6 5 5 e 90000000 0 0 0 0 0 O6 B 5 e 0000000 00 po 8 0 9 0 9 5 5 b oo0000 0 0 O0 O O O O O O9
49. 9 5 9 e 9 eoo 0000 06 O O O O 9 9 ee ee ee e 0 O 0 00 O ls OO O0 5B B 9 P 9 9 9 9 h b 9 aR eo 00 0 0 06 O O O O 9 9 9 9 q b 0 O0 O9 O O O O O O O 9 9 9 9 9 0 0 0 00 O06 0 O O O O 9 B 9 8 6 5 5 ee Le 0 06 O0 O6 O O O O O O 9 O9 9 9 9 9 a h e o 6 O OB O O O O9 P O 9 a 9 9 9 b 9 h n k hk c5 oc o 0 060 O WWW O O9 9 9 9 9 9 b Q 9 c c 6 0 0 O6 0 O 5B 0 0 OB 6 DP B B B b B c c c P e O0 O5 9 B O9 O 9 P P terme WWW e 0o 6 O O O9 OO ee goe e e e eg e ge c c 0oo0 00 00 5 6 amp 9 e i a e ee Zeie O O 0 0 0 0 o o o o et Ba e d e 1 nAunes t t 9 9 9 9 9 5 8 0 A o tota Ze 0 0X eege 06668666 000000000000 000000000096 0000000000 000000000000 0 O p O BD 8 LI any P 48 0 eelef af 0 0 0 2 a 0 0 0 a 5b e e e e EDU U s SS SS D e b
50. 99 Mini transblotter R f 440000 Slim blotter R f 504021 PACK C R f 3410PC COMPREND MAXIGEL ECO 2 PS 305 XL UN PORTOIR GRATUIT POUR L ACHAT DE DEUX PEIGNES SUPPL MENTAIRES EN acnes NUCLEIQUES MAXIGEL THERMO 2 APPLICATION Cuve recommand e pour des gels pr paratifs ou analytiques sur gels de 20 x 24 cm Convient egalement pour la separation des ADN et ARN RFLP Screening des PCR sur 3 x 96 chantillons avec 7 peignes Ref 334421 compatibles pipettes multicanaux Longueur de s paration 12 cm ou 24 cm selon le mod le de portoir choisi e Nombre maximum d chantillons 392 avec 8 peignes Ref 334421 ou 8 peignes Ref 334422 e Portoir 20 x 24 cm recevant 8 peignes e Portoir 20 x 12 cm recevant 2 peignes Peignes 10 20 30 dents et pr paratifs Double peignes 44 21 dents UN PORTOIR GRATUIT POUR L ACHAT DE DEUX PEIGNES SUPPL MENTAIRES compatibles avec pipettes multicanaux e 4 pieds r glables et niveau bulle quip d un plateau double e Bandes rouges pour meilleure enveloppe pour connexion visualisation pendant le d p t des d un cryostat permettant le chantillons refroidissement du tampon par e Ports pour circulation de tampon change thermique Volume de tampon 1800 ml Ports pour connexion du cryostat Dimensions de la cuve Ports pour circulation du tampon Lx p x h 28 x37 x 9 cm 331000 MAXIGEL THERMO 2 Fournie portoir transparent aux U V 20 x
51. ACTERISTIQUES Volume de tampon 800 ml Dimensions de la cuve Lx p x h 28x 16x 9 cm REFERENCES DE COMMANDE 321000 MIDIGEL 2 Fournie avec Portoir tranparent aux U V 10 x 14 cm Lx L avec 2 parois amovibles pour coulage du gel peigne 16 dents 2 0 mm d paisseur volume des chantillons 24 ul couvercle de s curit cordons d alimentation accessoires pour circulation de tampon notice d emploi 3210PF PACK F Comprend MIDIGEL 2 ci dessus R f 321000 et g n rateur PS 304 XL 320102 Peigne 2 0 mm 10 48 ul 6 0 mm 320162 Peigne 2 0 mm 16 24 ul 3 0 mm 320121 Peigne pr paratif 1 0 mm 1 chantillon 2 marqueurs 360 uU 2 x 12 ul 90 0 3 mm D 340500 Base de coulage de gel avec niveau a bulle et pieds r glables pour 1 gel de 5 mm d paisseur compatible avec pipettes multicanaux CUVES POUR ACIDES NUCLEIQUES E 8 NOMBRE DE PEIGNES ET DIMENSIONS DES GELS AS KS 10 cm gt NM X gt gt A gt 7 lt u A 14 8cm Ze Portoir Ref 321100 Portoir R f 321050 Peignes en Delrin pour 4 peignes pour 2 peignes GENERATEURS CONSEILLES Page 62 APELEX PS 304 XL APELEX PS 305 XL x E 0 eee ad 99 09 0 e 10 090 SC PS 304 XL Ref 160600 PS 305 XL Ref 170500 ire TE CUVES DE TRANSFERT CONSEILLEES Pages 98 99 Mini Transblotter Ref 430000 Slim blotter R f 504001 PACK E R f 3210PF COMPREND MIDIGEL
52. Electro phoresis ug V ee gt x e e DX OX XC Ki Ss oaget NX X x ee x x x x x a x ww X APELEX NN ational lagnostics Cor Electrophoresis applications visit us on the Web Fundamental Principles of Electrophoresis Nucleic Acids Proteins The Dynamics of Gel Electrophoresis Sample Mobility Electrophoresis System Dynamics Ohm s Law The Matrix Buffers Homogeneous Buffer Systems Multiphasic Buffer Systems Isotachophoresis Buffer Additives The Electrophoresis Apparatus Gel Electrophoresis of DNA amp RNA Denaturing Polyacrylamide Gel Electrophoresis of DNA amp RNA Sample Preparation Gel Preparation Run Conditions Buffer Buffer Gradients Applications of Denaturing Polyacrylamide Gel Electrophoresis of DNA and RNA Molecular Weight Determination Manual Sequencing Maxam amp Gilbert Sequencing Sequencing Sanger Method Gel Electrophoresis for DNA Sequencing Automated Sequencers Differential Display RNA Mapping S1 Mapping Ribonuclease Protection Primer Extension Analysis of DNA Protein Interaction DNase footprinting In situ DNase Footprinting Methylation Interference Assay Uracil Interference Assay Native Polyacrylamide Gel Electrophoresis of DNA Sample Preparation Gel Preparation Applications of Native DNA PAGE PCR Analysis Mobility Shift Assay DNA RNA Purification from PAGE Gels Conformational Analysis Heteroduplex Analysis SSCP Analysis Ag
53. Gel 29 1 1 Liter Ready to use monomer Premixed 40 Acrylamide Bisacrylamide solution for the preparation of electrophoresis gels for Protein separation hagnostic diagnostic EC 834 450 SequaGel 4 450 ml EC 832 450 SequaGel 4 25 450 ml EC 837 450 SequaGel 4 75 450 ml EC 836 450 SequaGel 6 450 ml EC 838 450 SequaGel 8 450 ml EC 841 90 SequaGel Complete Buffer 90 ml Consist of one bottle of SequaGel monomere solution and SequaGel complete Buffer solution Simply mix 4 parts of bottle one with 1 part of bottle two to formulate 19 1 denaturing DNA gels of constant percentage EC 833 SequaGel Sequencing System 1 Kit EC 840 450 SequaGel Diluant 450 ml EC 830 450 SequaGel Concentrate 450 ml EC 835 SequaGel Buffer 100 ml Three bottle system to cast 19 1 sequencing gels from 4 up to 20 quickly and conveniently EC 847 SequaGel MD Heteroduplex 1 Kit EC 846 SequaGel MD SSCP 1 Kit EC 845 SequaGel MD Monomer Solution 200 ml For the detection of minor mutational differences in SSCP analysis and Heteroduplex analysis EC 842 450 SequaGel XR EC 843 100 SequaGel XR Concentrate National Diagnostics proprietary extended read matrix EC 850 1000 AccuGel 19 1 Ready to use monomer solutions for the preparation of electrophoresis gels for DNA or RNA EC 810 000 AcrylaGel 1 Liter AcrylaGel is a 30 stabilized ready to use acrylamide solution EC 820 1000 Bis AcrylaGel 1 Liter Bis AcrylaGel is a ready to use 2 solution of met
54. IE Apr s coupure automatique du d bit par la minuterie les bandes peuvent diffuser dans le gel Sans activer la fonction GEL SAVER importante diffusion des bandes quatre heures apr s la coupure du d bit Ce led indique le fonctionnement de la minuterie Pour viter la diffusion S lectionner la fonction GEL SAVER l aide de ce switch avant ou pendant la migration Ce led indique le fonctionnement du GEL SAVER Pas de risque de diffusion Apr s coupure automatique du d bit par la minuterie un courant minimum r gul de faible valeur est automatiquement d bit Il maintient les bandes align es et nettes A n R sultat obtenu GRACE AU GEL SAVER quatre heures Comparer avec la photo 3 la apres la coupure du debit s paration ci dessus obtenue par la minuterie juste apres coupure du debit par la minuterie CARACTERISTIQUES GENERALES Les derniers param tres programm s sont e Un param tre peut tre programm pour r guler durant automatiquement m moris s toute La migration e Panneaux membranes avec switches tactiles pour e Coupure automatique avec alarme sonore en cas de fuite r glages pr cis des param tres lectrique la terre court circuit circuit ouvert cuve e Nettoyage facile d branch e ou non branch e surcharge soudaine e Voltage ou courant constant avec changement e Apres coupure et retour du courant secteur une alarme automatique
55. L XL Fournie avec 8102325 Portoir transparent aux U V 23 x 25 cm 1 x L recevant jusqu 10 peignes 810125 2 x peignes 25 dents paisseur 1 mm compatible pipettes multicanaux 810150 2 x peignes 50 dents paisseur 1 mm compatible pipettes multicanaux 340600 Base de coulage de gel avec pieds r glables et niveau bulle Compatible avec portoirs 23 x 40 cm et 23 x 25 cm 810270 Couvercle de s curit avec cordons d alimentation 810280 Accessoires pour circulation de tampon 812340 MAXIGEL XXL Fournie avec 8112340 Portoir transparent aux U V 23 x 40 cm 1 x L recevant jusqu 12 peignes 810125 2 x peignes 25 dents paisseur 1 mm compatible pipettes multicanaux 810150 2 x peignes 50 dents paisseur 1 mm compatible pipettes multicanaux 340600 Base de coulage de gel avec pieds r glables et niveau bulle Compatible avec portoirs 23 x 40 cm et 23 x 25 cm 811270 Couvercle de s curit avec cordons d alimentation 810280 Accessoires pour circulation de tampon 8100PH PACK H Comprend G n rateur PS 608 MAXIGEL XXL equip e comme suit 8112340 Portoir transparent aux U V 23 x 40 cm 1 x L recevant jusqu 12 peignes 8102325 Portoir transparent aux U V 23 x 25 cm 1 x L recevant jusqu 10 peignes 8115242 8 x peignes 26 dents paisseur 1 5 mm 24 2 marqueurs compatibles avec pipettes multicanaux 8125SLG Guide de d p t des chantillons pour peignes 26 dents 340600 Base de coulage de gel avec pieds r glables et niveau bulle
56. LE COULAGE MONTAGE TRES RAPIDE DES PLAQUES DE VERRE AVEC ESPACEURS JOINTS LAT RAUX D ETANCHEITE EN NEOPRENE e POUR LA MIGRATION INSTALLATION DES GELS DANS LE MODULE EN 2 SIMPLES CLICS e MODULE DE MIGRATION AVEC ELECTRODES PLATINE PROTEGEES e FOURNIE COMPLETE POUR COULER ET MIGRER 2 GELS 10 x 10 CM PAISSEUR 0 8 MM e RECOIT GALEMENT DES GELS PR COUL S 10 x 10 CM ET 8 x 10 CM e VOLUME DE TAMPON 350 850 ML e COUVERCLE AVEC SYSTEME DE VENTILATION BREVET e NETTOYAGE FACILE DE LA CUVE CUVES POUR PROTEINES EE 2 ON L Pe 22 Mt eg gt s MONTAGE R F RENCES DE COMMANDE 1 Former un sandwich avec espaceurs et plaques de verre Ma GEL n complete 2 Placer le sandwich dans le support de coulage Fournie avec Module central et cuve de migration plaque avec encoche vers l int rieur Couvercle ventil Support de coulage 3 plaques de verre avec encoche et 3 plaques de verre pleines 2 jeux d espaceurs 0 8 mm 2 peignes 15 dents 0 8 mm molettes laterales cordons et notice 4 Couler le gel entre les plaques et ins rer les peignes be Apr s polym risation retirer les peignes avec pr caution 92030 0 8 mm x 10 dents 5 86 mm 28 13 ul 6 Apr s r cup ration des sandwiches les glisser verticalement dans le module de migration 92033 0 8 mm x 1 dent 70 mm 336 ul 3 Exercer une pression sur le couvercle et visser les 7 Repousser les pinc
57. PERFORMANCE BETWEEN 8 150 kDa 0 050 0 176 TR 10110 10 Tricine Gel 1 0 mm 10 well 10 pieces in a box n 0 653 10112 10 Tricine Gel 1 0 mm 12 well 10 pieces in a box 0 887 TR 10115 10 Tricine Gel 1 0 mm 15 well 10 pieces in a box erg IDEAL PERFORMANCE BETWEEN 1 40 KDa 4 M TR 16110 16 Tricine Gel 1 0 mm 10 well 10 pieces in a box TR 16112 16 Tricine Gel 1 0 mm 12 well 10 pieces in a box TR 16115 16 Tricine Gel 1 0 mm 15 well 10 pieces in a box IDEAL PERFORMANCE BETWEEN 2 200 kDa Overall Performance Pass TR 12010 10 20 Tricine Gel 1 0 mm 10 well 10 pieces in a box Or e ls TR 12012 10 20 Tricine Gel 1 0 mm 12 well 10 pieces in a box Waj Beie TR 12015 10 20 Tricine Gel 1 0 mm 15 well 10 pieces in a box Sas AN S n a A d ANAMED NUCGEL TRIS BORATE EDTA TBE GELS FOR ELECTROPHORESIS OF DNA TB 06110 TB 06112 TB 06115 TB 08110 TB 08112 TB 08115 TB 10110 TB 10112 TB 10115 TB 20110 TB 20112 TB 20115 TB 41210 TB 41212 TB 41215 TB 42010 TB 42012 TB 42015 IDEAL PERFORMANCE BETWEEN 6 TBE Gel 1 0 mm 10 well 6 TBE Gel 1 0 mm 12 well 6 TBE Gel 1 0 mm 15 well IDEAL PERFORMANCE BETWEEN 8 TBE Gel 1 0 mm 10 well 8 TBE Gel 1 0 mm 12 well 8 TBE Gel 1 0 mm 15 well IDEAL PERFORMANCE BETWEEN 10 TBE Gel 1 0 mm 10 well 10 TBE Gel 1 0 mm 12 well 10 TBE Gel 1 0 mm 15 well IDEAL PERFORMANCE BETWEEN 20
58. RT DE L ADN S lectionner les cuves avec anode en Titane platine NORTHERN BLOT TRANSFERT DEL ARN S lectionner les cuves avec anode en Titane platine LES PLUS DE LA GAMME SLIM BLOTTERS e Western Blots avec gel de 1 mm 30 minutes 24 Volts e Faible volume de tampon Pas d entretien sur les lectrodes en Titane Platine e Simple nettoyage des lectrodes en acier e Chargement de la cuve en quelques minutes Excellent champs lectrique entre les lectrodes e 5 mod les avec lectrodes en acier pour les WESTERN BLOT e 5 mod les avec anode en Titane Platin pour SOUTHERN NORTHERN et WESTERN BLOTS e Pas de formation de bulles sur les lectrodes REFERENCES DE COMMANDE 504001 SLIM BLOTTER avec lectrodes 10 x 15 cm en acier pour Western Blots Volume de tampon 350 ml 504015 SLIM BLOTTER avec lectrodes 15 x 20 cm en acier pour Western Blots Volume de tampon 800 ml 504020 SLIM BLOTTER avec lectrodes 20 x 24 cm en acier pour Western Blots Volume de tampon 1 400 ml 504040 SLIM BLOTTER avec lectrodes 25 x 34 cm en acier pour Western Blots Volume de tampon 2 500 ml 504002 SLIM BLOTTER avec lectrodes 10 x 15cmdont 1 en Titane Platin pour Southern Northern et Western Blots Volume de tampon 350 ml 504017 SLIM BLOTTER avec lectrodes 15 x 20 cm dont 1 en Titane Platin pour Southern Northern et Western Blots Volume de tampon 800 ml 504021 SLIM BLOTTER avec lectrodes 20 x 24 cm do
59. S Running buffer for VarioGel 20 x Concentrate 500 ml VM 10509 MOPS Tris SDS Running buffer for VarioGel 20 x Concentrate 1000 ml VM 50509 MOPS Tris SDS Running buffer for VarioGel 20 x Concentrate 5000 ml VT 05508 Tricin Tris SDS Running buffer for VarioGel 20 x Concentrate 500 ml VT 10508 Tricin Tris SDS Running buffer for VarioGel 20 x Concentrate 1000 ml VT 50508 Tricin Tris SDS Running buffer for VarioGel 20 x Concentrate 5000 ml VP 02510 Tris HCUSDS Samplebuffer for VarioGel 2 x Concentrate 20 ml VP 05510 Tris HCUSDS Samplebuffer for VarioGel 2 x Concentrate 50 ml TB 01075 TBE Samplebuffer for DNS Samples 5 x Concentrate extra heavy 10 ml BUFFER SOLUTIONS FOR BLOTTING THE ANAMED PRECAST GELS TG 05005 Transfer buffer for VarioGel ProGel ProGel P 25 x Concentrate 500 ml TG 10005 Transfer buffer for VarioGel ProGel ProGel P 25 x Concentrate 1000 ml 0 2 ANAMED NEW PRECAST GELS FAMILY THE VARIOGEL Two variations 9 and 4 12 VarioGel for precise Protein or DNA separation VarioGel is a pH neutral Polyacrylamide Precast Gel designed for the vertical SDS Electrophoresis in format of 10 cm x 10 cm gels VarioGel is clearly designed for being an alternative product to other available long shelf life gels VarioGel doesn t need an antioxidant added to the run buffer solution to prevent reoxidation of the samples during run time of the electrophoresis it s already in
60. TART mode turns on automatically at the preset values Recommended for over night electrophoresis and sequencing Automatic storage of the preset and elapsed values minutes and in case of power failure or voluntary interruption during the cycle This microprocessor controlled power supply is equipped with a circuit breaker which automatically cuts the electrical supply in case of ground leakage detection 500 pA short circuit open circuit or overload Specific messages are displayed POWER SUPPLIES TEMPERATURE CONTROL The power supplies are designed for automatic temperature regulation as well as that for Voltage Current and Power The optional temperature probe maintains constant temperature during electrophoresis by reducing or stopping the voltage and current output when the set temperature is surpassed Once the temperature drops below the set point both voltage and current output is resumed at the set levels The use of the temperature regulation mode prevents the activation of the power Watt regulation mode The display Min R is a dedicated timer for increments of minutes while the power supply is monitored by the temperature function This timer increments minutes only when voltage and current are turned off during the temperature regulation mode The main timer Min T remains operational at all times during the operation of the power supply and provides the automatic termination of the voltage and
61. TBE Gel 1 0 mm 10 well 20 TBE Gel 1 0 mm 12 well 20 TBE Gel 1 0 mm 15 well IDEAL PERFORMANCE BETWEEN 4 12 TBE Gel 1 0 mm 10 well 4 12 TBE Gel 1 0 mm 12 well 4 12 TBE Gel 1 0 mm 15 well IDEAL PERFORMANCE BETWEEN 4 20 TBE Gel 1 0 mm 10 well 4 20 TBE Gel 1 0 mm 12 well 4 20 TBE Gel 1 0 mm 15 well 60 2500 bp 10 pieces in a box 10 pieces in a box 10 pieces in a box 40 2500 bp 10 pieces in a box 10 pieces in a box 10 pieces in a box 50 1500 bp 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 900 bp 10 pieces in a box 10 pieces in a box 10 pieces in a box 60 2500 bp 10 pieces in a box 10 pieces in a box 10 pieces in a box 20 3000 bp 10 pieces in a box 10 pieces in a box 10 pieces in a box NUCGEL S TBE UREA GELS FOR THE ELECTROPHORESIS OF SINGLE STRANDED DNA OR RNA TU 06110 TU 06112 TU 06115 TU 10110 TU 10112 TU 10115 TU 15110 TU 15112 TU 15115 IDEAL PERFORMANCE BETWEEN 6 TBU Gel 1 0 mm 10 well 6 TBU Gel 1 0 mm 12 well 6 TBU Gel 1 0 mm 15 well IDEAL PERFORMANCE BETWEEN 10 TBU Gel 1 0 mm 10 well 10 TBU Gel 1 0 mm 12 well 10 TBU Gel 1 0 mm 15 well IDEAL PERFORMANCE BETWEEN 15 TBU Gel 1 0 mm 10 well 15 TBU Gel 1 0 mm 12 well 15 TBU Gel 1 0 mm 15 well 40 750 bases 10 pieces in a box 10 pieces in a box 10 pieces in a box 20 500 bases 10 pieces in a box 10 pieces in a box 10
62. X individuel rigoureux atteste Les g n rateurs APELEX ont t concus en respect que le g n rateur est sont faciles utiliser Leur des normes de s curit conforme nos crit res de manipulation est simple lectriques courantes qualit et de performance permettant une mise en Ils sont conformes aux APELEX offre une garantie de route rapide Les param tres normes internationales 3 ans sur ses g n rateurs sont clairement affich s et et sont labellis s CE des messages d di s guident efficacement l utilisateur en cas de dysfonctionnement avant et pendant la migration Un Lk D UI lt LK LU I LL Q 2 O C2 Li lt CD 415504 enesuo m 1 2 5 5 1 1 sjneJedaad 5196 ya SAS saulajoid sep s s JoudoJ 5 13 499d 4255 20 01128805 s nbi o2nN abesuanbas 25 5 d7144 NYV NGV sep 2S2104d0112913 MENS RE WW GE REN m m m m m y Lp eS Ss a qewwesboid 1n e1p U JoAeS 199 11 NUIW 91Uejsuoo e2uessind ja jueJno2 jue1suo jue31suo JULINOY 26e 0A 00 e uessing Wu
63. XL Cat No 160600 PACK 965 Cat 372696 Tank MIDIGEL XL 96 samples Cat No 371000 Power Supply PS 304 XL Cat 160600 PACK B Cat No 3760PB Tank MIDIGEL XL Cat No 370000 Power Supply PS 304 XL Cat No 160600 PACK Cat No 3420PC Tank MAXIGEL ECO 2 Cat No 341000 Power Supply PS 305 XL Cat 170500 PACK F Cat No 3220PF Tank MIDIGEL 2 Cat No 321000 Power Supply PS 304 XL Cat No 160600 PACKG Cat No 3320PG Tank MAXIGEL THERMO 2 Cat No 331000 Power Supply PS 305 XL Cat 170500 PACK H Cat No 8100PH Tank MAXIGEL XXL Cat No 812340 Power Supply PS 608 Cat No 173000 A dis P SUBMARINE AGAROSE GELS ROBUSTNESS Tanks lids and gel trays are made of 10 or 6 mm thick acrylic No injection molded part no warping in the event of too hot media being poured or overheating running situations QUALITY A wide range of precision made DELRIN combs LIFE GUARANTIED Many thicknesses and tooth configurations accomodate many samples including compatibility with multichannels pipettes or large sample volume EASE OF USE U V transparent gel trays which enable to view ethidium bromide stained gels Include a fluorescente rule SC TANKS FOR NUCLEIC ACIDS When the lid is closed power cords must be completly removed to allow the opening White gel table with bright Levelling feet at each corner and red visualization strips to levelling bubble allow perfect long enhanc
64. alarm message stop audible alarm message Operating temperature C cc UAE 74086 0 40 Dimensions w x d x h 18x21x8cm 18x21x8cm 18x21x8cm Weight 1 8 kg 1 8 kg 1 8 kg POWER SUPPLIES ps 503 XL amp PS 305 XL WHAT IS THE GEL SAVER FUNCTION AVOIDING DIFFUSION OF BANDS AFTER THE RUN After the timer cut off the run the bands start to diffuse In the gel See diffusion of bands four hours after the run This led is alighted when timer is ON In order to avoid diffusion depress the Gel Saver Switch when setting the timer This led is alighted when Gel Saver is ON No risk of band diffusion when the function GEL SAVER 15 ON After the timer has cut off the run a constant low voltage Is automatically supplied until the STOP switch 15 depressed AA I MN M Gel saver made a Good Job Bands are still on line 4 hours after the timer has cut off the run Compare with separations shot just after the run POWER SUPPLIES FEATURES Last settings are automatically restored at power up It is possible to perform electrophoresis with constant current whilst limiting the voltage to a selected value Tactile membrane panel for precise easy input and vice versa Automatic cut off in case of ground of parameters Easy to clean leakage detection s
65. ant 15 45 mA gel 4 x plain glass plate 2 x 0 8 mm 20 wells Teflon combs maximum e Maximum voltage 300 Volt sample volume 97 ul 1 blind plate 2 x 0 8 mm 15 wells Teflon e Run time 30 90 minutes combs maximum sample volume 144 ul 2 sets of 0 8 mm thick spacers 2 replacement gaskets Gel casting base including 2 silicone seals 2 cams TECHNICAL SPECIFICATIONS 2 x replacement silicone seals instruction manual Total Buffer Volume 600 800 ml Maximum number of samples 2 x 25 Range of combs and accessories on page 39 Unit dimensions 0 15 x 28 x 13 3 TANKS FOR PROTEINS SUGGESTED POWER SUPPLIES Page 6 PS 304 XL 9 909 Se L9 99 0 zo PS 304 XL Cat No 160600 PS 305 XL Cat No 170500 SUGGESTED BLOTTING TANKS Pages 42 44 Large Transblotter Cat No 440000 Slim Blotter Cat No 504015 Large Semi Dry Blotter Cat No 460000 jj AN PROTEINS MIDI VERTIGEL 2 Seit a a IDEAL FOR RAPID SEPARATION DESCRIPTION 480000 MIDI VERTIGEL 2 comes fully equipped for casting and running 2 gels Active gel sizes 15 x 12 5 cm W x H Includes Main buffer tank with safety lid and attached connecting Glass plate sizes 16 cm W x 14 16 cm L leads 1 upper twin running module equipped by water cooled Acrylamide capacity 13 5 26 9 ml integral cooling chamber 4 x notched glass plate Current constant 15 50 mA gel 4 x plain glass plate 2 sets of 1
66. arose Gel Electrophoresis of DNA and RNA Preparation of Agarose Gels Preparation of Denaturing Agarose Gels Applications of Agarose Gel Electrophoresis www apelex fr Restriction Digest Mapping DNA RNA Purification from Agarose Gels Electroelution In Gel Enzyme Reactions Pulsed Field and Field Inversion Gel Electrophore RNA Electrophoresis Preparation of RNA Samples Gel Electrophoresis of RNA Gel Electrophoresis of Proteins Denaturing Protein Electrophoresis SDS PAGE Sample Preparation Gel Preparation Denaturing Protein Gels Gradient Gels Applications of Denaturing Protein Electrophoresis Measuring Molecular Weight Gradient SDS Gels Peptide Mapping Analytical Gels for Peptide Mapping Protein Purification using Denaturing Electrophon Antibody Production from PAGE Purified Proteins Native Protein Electrophoresis Ferguson Plots Native Gradient Gels Sample Preparation Native Protein Electrophore Cell and Tissue Disruption Gel Preparation Native Protein Gels Native Gradient Gels Applications of Native Protein Gels Activity Stains Immuno Electrophoresis Immune Diffusion Two Dimensional Electrophoresis Isoelectric Focusing Post Electrophoretlc Analysis DNA and RNA Detection Staining of Nucleic Acids Blotting Nucleic Acids Northerns and Southerns Transfer Techniques Autoradiography Protein Detection Fixing Proteins on Electrophoresis Gels Staining Proteins in Gels Guide Strip Technique Staining of Proteins Immobi
67. ates two sets of 0 8 mm side spacers Secure lid by pressing down and inserting locking knobs two 0 8 mm x 15 tooth combs power cords and manual Ae Add gel through opening in lid Description Well Wildth Sample Volume 5 Carefully remove combs Per mm Gel 6 Insert gel plates in gel capture device per instructions 92030 0 8 mm x 10 tooth 5 86 mm 28 13 ul 7 Turn cams inward to secure gel plates 92031 0 8 mm x 12 tooth 4 22 mm 20 26 ul o _ 92032 0 8 mm x 15 tooth 3 38 mm 16 22 ul 8 Place gel capture device tank note keyed inserts 92033 EEE 70 mm 336 ul 9 e Pour appropriate amount of buffer 10 Load the samples in the wells SUGGESTED POWER SUPPLY ES EECHER PS 304 300 Volts 400 mA Cat No 160500 Page 6 TECHNICAL SPECIFICATIONS CONSTRUCTION ORDERING INFORMATION Clamp assemblies Molded polycarbonate e Buffer chamber 3 8 acrylic Gel size 8 cm L x 10 cm atre The 92000 comes with a patented vented lid Patent Cat No 5779 869 92200 Protein Casting System 8 Place 92500 Western Transfer Module Tank BLOTTING MINI GEL TRANSBLOTTER FOR UP TO 4 GELS 10 x 10 cm e Water cooled base with stirring bar which can be placed in the unit under the compression cassette Then unit is placed on a stable magnetic stirrer e An open squarre grid design for the cassettes provides a maximum area for the transfer and is strong enough to maintain a firm contact between the gel and
68. c cordons solidaires 2 joints de remplacement notice Peigne 10 dents 1 5 mm volume des puits 449 ul Largeur des dents 13 mm Peigne 15 dents 1 5 mm volume des puits 271 ul Largeur des dents 8 mm Peigne 20 dents 1 5 mm volume des puits 182 ul Largeur des dents 5 5 mm Peigne 25 dents 1 5 mm volume des puits 129 ul Largeur des dents 4 mm Peigne pr paratif 2 dents 1 5 mm volume des puits 4230 ul 135 pl Largeur des dents 151 5 mm Espaceurs 1 5 mm x 2 Peigne 10 dents 0 8 mm volume des puits 239 pl Largeur des dents 13 mm Peigne 15 dents 0 8 mm volume des puits 144 ul Largeur des dents 8 mm Peigne 20 dents 0 8 mm volume des puits 97 ul Largeur des dents 5 5 mm Peigne 25 dents 0 8 mm volume des puits 69 ul Largeur des dents 4 mm Espaceurs 0 8 mm x 2 Plaque de verre pleine 20 x 20 cm x 1 Plaque de verre avec encoche 20 x 20 cm x 1 Paroi d obturation pour migration d un seul gel Module de s paration avec lectrodes fourni avec la cuve R f 420000 Pinces de rechange pour module R f 420010 x 2 Joints de rechange pour module R f 420010 x 2 Base de coulage de gel d j inclus dans la r f rence R f 420000 Sachet de 2 joints pour syst me de coulage de gel R f 420020 Base de coulage de gel module de s paration 79 7 PROTEINES e ei 7 I wi UA 92000 MAeGELeIN MONTAGE RAPIDE ET FIABLE e POUR
69. current output After the electrophoresis has automatically stopped it may be necessary to continue electrophoresis for the time that the voltage was discontinued Display 1 e Volt mA Watt e Integrator Timer for automatic cut off Display 2 Volt mA Temperature limit e Dedicated timer indicating In minutes the duration of the temperature control timing e Timer for automatic cut off Cat No 119020 Optional probe for temperature control PS 9009 TX PS 1006 P Output voltage range Resolution Output current range Resolution Output current range with Prog 28 Output power range Resolution Temperature range Resolution Integrator range Resolution Timer range Resolution Minimum regulated value Value display range Resolution SPECIFICATIONS Minimum non regulated value Minimum output with program 28 10 3000 Volt 10 Volt steps 15 pA 300 mA 1 mA steps mA 300 mA 0 3 300 watts 1 watt steps 0 99 C in 1 C steps 1 99999 Volt Hr 1 Volt Hr steps 1 9999 minutes 1 minute steps 10 Volt 1 mA 1 watt 0 3000 Volt 10 Volt 0 300 mA 1 mA 0 300 watts 1 watt D 7726 T9E 0 99999 Volt Hr 1 Volt Hr steps 0 9999 minutes 1 mn steps Volt 15 pA 0 3 W 10 Volt 0mA 0 W 10 1000 Volt 10 volts steps 15 pA 600 mA 1 mA steps 0 3 300 watts 1 watt steps 0 99 1 C steps 1 99999 Volt Hr 1 Vol
70. d a gel buffer will allow more current to flow leading to more heat generation with distortions such as smiling Too dilute a gel buffer will increase resistance to current flow which may actually sharpen the bands but generally slows the run and reduces resolution WWW APELEX FR USUAL INFORMATION FOR ELECTROPHORESIS Effective Range of Separation of DNAs and Effective Range of Separation of DNAs and Dye Co Migration in Native Polyacrylamide Gels Dye Co Migration in Denaturing Polyacrylamide Gels Size Bromophenol Blue Xylene Cyanol Size Bromophenol Blue Xylene Cyanol Gel Range bp nucleotides nucleotides Gel Range bp nucleotides nucleotides 35 55 8 10 50 300 120 10 20 60 10 The sieving characteristics of Aragose Gels different types of agarose vary Gia considerably Gel Range bp A more complete discussion can be found in the web site 1 5 200 3000 2 0 100 2000 Lys Lys Asn Aen Arg Arg Ser Met Thr Thr Thr Lys Lys Asn Aen Arg Arg Ser Met Thr Thr Thr Range of Separation of Proteins in SDS PAGE A DA KE EE 7 EIA Lys Lys Asn Asn Arg Arg Ser lle Met Thr Thr Thr C AG CA T of CEIA Lys Lys Asn Aen Arg Arg Ser Met Ile Thr Thr Thr Thr Size Gel Range kp DNA Data Melting Point Calculations Base pairs per turn B form 10 TMpna RNA 79 8 C
71. d control the output relative to that parameter Automatic RESTART in case of power failure during a cycle When the power returns an audible safety alarm rings for 10 seconds then the START mode turns on automatically at the preset values These microprocessor controlled power supplies are equipped with a circuit breaker which automatically cuts the electrical supply in case of ground leakage detection 500 pA short circuit open circuit no load connected or overload SPECIFICATIONS PS 608 1 600 volt 1 volt steps 1 800 mA 1 mA steps Output range Resolution Current Output range Resolution Timer range Resolution 0 9999 min 1 min steps 0 9999 min 1 min steps PS 1006 2 998 volt 2 volt steps 1 600 mA 1 mA steps PS 202 1 200 volt 1 volt steps 2 2000 mA 2 mA steps 0 9999 min 1 min steps Power 0 3 300 watts 0 3 300 watts 0 3 300 watts Displays 9 3 3 Minimum regulated value 5 volt 1mA Minimum non regulated value 1V 15 pA 0 3 W Fault detection Operating temperature G A el 10 volt 1mA 5 volt 2 mA 2V 15pA 0 3W 15 0 3 stop audible alarm fault Led stop audible alarm fault Led stop audible alarm fault Led 0 C 40 C 0 C 40 C Dimensions w x d x h 27 x34 x 11 cm 27 x 34x 11 cm 27 x 34 x 11 cm Weight 4 kg 4 kg 4 kg APPLICATIONS DNA RNA Electrophoresis agarose gel RFLP a
72. d glass plates 4 plain glass plates 1 blind plate 2 x 15 wells 1 5 mm combs 2 x 20 wells 1 5 mm combs 4 spacers 1 5 mm 2 replacement gasket internal running module with internal cooling chamber safety lid with power cords gel casting base user s manual 10 teeth comb 1 5 mm Well volume 346 ul Teeth wide 10 4 mm 15 teeth comb 1 5 mm Well volume 201 yl Teeth wide 6 1 mm 20 teeth comb 1 5 mm Well volume 129 ul Teeth wide 3 9 mm 24 teeth comb 1 5 mm Well volume 96 yl Teeth wide 2 9 mm Prep comb 2 teeth 1 5 mm Well volume 1700 pl Teeth wide 120 5 mm Set of 2x 1 5 mm spacers 10 teeth comb 0 8 mm Well volume 183 ul Teeth wide 10 4 mm 15 teeth comb 0 8 mm Well volume 107 ul Teeth wide 6 1 mm 20 teeth comb 0 8 mm Well volume 69 ul Teeth wide 3 9 mm 24 teeth comb 0 8 mm Well volume 51 ul Teeth wide 2 9 mm Set of 2x 0 8 mm spacers Plain glass plate 16 x 14 cm W x H 1 piece Notched glass plate 16 x 14 cm W x H 1 piece Blocking plate for running one gel Blind plate Water cooled removable Running module already included in the package Cat No 480000 Replacement clamps for running module Cat No 480010 x 2 Replacement gasket for running module Cat No 480010 x 2 Gel casting base already included in the package Cat No 480000 Replacement gasket for casting base Cat No 480020 x 2 Gel casting base running module TANKS FOR PROTEINS VERTIGEL 2 410000 411015 411515 412015
73. de r gulation en fonction des pr r glages sonore se d clenche pendant 10 secondes puis le Deux leds indiquent le type de r gulation en cours g n rateur relance son d bit automatiquement en e IL est possible de programmer une r gulation de voltage respect des valeurs programm es Volts mA et minutes avec limitation du courant et inversement restant avant la coupure du secteur x NCIS A s PUISSANTS FACILES A UTILISER ET ROBUSTES APELEX rie e Voltage Ten Valls e LE 5 250 Walls PS 202 pourte Biotting R f 176000 200 Volts 1998 mA PS 608 R f 173000 600 Volts 800 mA PS 1006 suisses R f 174000 1000 Volts 600 mA FACILE UTILISER PS 1006 Electrophoresis Power Supply 1 DANGER HIGH VOLTAGE GENERATEURS CARACTERISTIQUES GENERALES Les derniers param tres programm s sont e Un param tre peut tre programm pour r guler durant automatiquement m moris s toute la migration e Coupure automatique avec alarme sonore en cas de fuite e Panneaux membranes avec switches tactiles pour lectrique a la terre court circuit circuit ouvert cuve r glages pr cis des param tres d branch e ou non branch e surcharge soudaine e Nettoyage facile e Apr s coupure et retour du courant secteur une alarme e Voltage ou courant constant avec changement sonore se d clenche pendant 10 secondes puis l
74. dents 0 8 mm volume des puits 55 ul Largeur des dents 4 3 mm Espaceurs 0 8 mm x 2 Plaque de verre pleine 10 x 10 cm x 1 Plaque de verre avec encoche 10 x 10 cm x 1 Plaque c ramique avec encoche 10 x 10 cm x 1 Paroi d obturation pour migration d un seul gel Module de s paration seul avec lectrodes fourni avec la cuve R f 400000 Pinces de rechange pour module R f 400010 x 2 Joints de rechange pour module R f 400010 x 2 Base de coulage de gel fourni avec la cuve R f 400000 Joints de rechange pour base de coulage x 2 Base de coulage de gel module de s paration Midi Vertigel 2 compl te pour 2 gels 15 x 12 5 cm 1 H paisseur 1 5 mm base de coulage de gels et joints de remplacement 4 plaques de verre avec encoche 4 plaques de verre pleines 16 x 14 cm 1 paroi d obturation 2 peignes 15 dents 1 5 mm 2 peignes 20 dents 1 5 mm 4 espaceurs 0 8 mm couvercle avec cordons solidaires 2 joints de remplacement notice Peigne 10 dents 1 5 mm volume des puits 343 pl Largeur des dents 10 4 mm Peigne 15 dents 1 5 mm volume des puits 201 ul Largeur des dents 6 1 mm Peigne 20 dents 1 5 mm volume des puits 129 ul Largeur des dents 3 9 mm Peigne 24 dents 1 5 mm volume des puits 96 ul Largeur des dents 2 9 mm Peigne pr paratif 2 dents 1 5 mm volume des puits 3200 150 ul Largeur des dents 120 5 mm Espaceurs 1 5 mm x 2 Peigne 10 dents 0 8 mm volume des puits 183 pl Largeur des dents 1
75. discontinuity which migrates through the gel distorting the band pattern with a wave effect This can also cause bands to migrate at an angle to the vertical causing the bands to look broad when viewed from directly above In some cases such bands will appear to be doublets A A ational lagnostics www nationaldiagnostics com D Matrix Related Problems Problems with the matrix will manifest as smeared bands and or loss of resolution Note that most total failures of electrophoresis are not matrix related see D Buffer related problems 1 2 3 4 Pore size This is determined by the amount of matrix and cross linker used suggested ranges are given on the web site Too small of a pore size will prevent proteins of interest from moving far enough into the resolving gel to be resolved Too large of a pore size will allow smaller proteins to run with the SDS front creating a large intense protein band containing many unresolved proteins running just behind or with the tracking dye Polymerization Polymerization of the gelis a chain reaction initiated by Ammonium Persulfate APS TEMED The extension of initiated chains is inhibited by oxygen APS and TEMED must be fresh for consistent results APS should crackle when dissolving TEMED should be clear with no yellow color Use of old reagents will lead to incomplete polymerization which 15 easily inhibited by low levels of dissolved oxygen
76. e automatique de regulation en fonction des pr r glages g n rateur relance son d bit automatiquement Deux leds indiquent le type de r gulation en cours respect des valeurs programm es Volts mA et minutes e l est possible de programmer une r gulation de voltage restant avant la coupure du secteur avec limitation du courant et inversement SP CIFICATIONS PS 608 PS 1006 PS 202 Gamme de tension R solution 1 600 volt Pas de 1 volt 2 998 volt Pas de 2 volts 1 200 volt Pas de 1 volt Gamme de courant R solution 1 800 mA Pas de 1 mA 1 600 mA Pas de 1 mA 2 2000 mA Pas de 2 mA Minuterie R solution 0 9999 min Pas de 1 min 0 9999 min Pas de 1 minute 0 9999 min Pas de 1 minute Puissance 0 3 300 watts 0 3 300 watts 0 3 300 watts Afficheurs 3 3 3 Valeurs minimum r gul es 5 volts 1mA 10 volts 1mA 5 volts 2 mA Seuils minimum de r gulation 15 0 3 W 2 1 5 0 3 15 0 3 D tection fautes stop alarme sonore Led faute stop alarme sonore Led faute stop alarme sonore Led faute Temp rature de fonctionnement 0 40 Ust 0 40 Dimensions lx p x h 27 x 34 x 11 cm 27 x 34 x 11 cm 27 x 34 x 11 cm Poids 4 kg 4 kg 4 kg APPLICATIONS PS 1006 ADN ARN lectrophor se sur agarose RFLP et Fragments ADN Screening PCR Electrophor se des Prot ines lectrophor se 2 D SDS Page Elec
77. e 5 5 mm 25 teeth comb 0 8 mm Well volume 69 ul Teeth wide 4 mm Set of 2x 0 8 mm spacers Plain glass plate 20 x 20 cm 1 piece Notched glass plate 20 x 20 cm 1 piece Water cooled removable Running module already included in the package Cat No 420000 Replacement clamps for running module Cat No 420010 x 2 Replacement gasket for running module Cat No 420010 x 2 Gel casting base already included in the package Cat No 420000 Replacement gasket for casting base Cat No 420020 x 2 Gel casting base running module y MEAWESPROTENS 0 00 Al 92000 MAeGELeIN UNIVERSAL CAST YOUR OWN SYSTEM The NEW Ma GEL In is truly a versatile protein system designed to accomodate today s researcher This system will allow you to cast your own gels using the 10 cm x 10 cm glass plates and easy to use casting system or if you prefer to use 8 cm x 10 cm precast gels the Ma GEL In will accommodate those as well This versatility comes from the specially designed patent pending gel capture system that allows plates in a 10 cm x 10 cm format or cast your own glass plates in a 10 cm x 10 cm format Casting your oun gels The 920000 comes with three sets of glass plates and a 2 place casting system for casting your own gels The 2 place casting system 921000 has a very small footprint 10 cm x 15 utilizing minimal space on the counter The glass plates are held together using neoprene spacers Once ass
78. e avec changement automatique de r gulation en fonction des pr r glages e Affichage imm diat du param tre constant Coupure automatique avec alarme sonore en cas de fuite lectrique la terre court circuit circuit ouvert cuve d branch e ou non branch e surcharge soudaine Un message s affiche simultan ment indiquant le type de faute l origine de la coupure du d bit Apr s coupure et retour du courant secteur une alarme sonore se d clenche pendant 10 secondes puis le g n rateur relance son d bit automatiquement en respect des valeurs programm es Volts mA Watts Le temps restant sur la minuterie est galement restaur ainsi que la valeur Volt heure de l int grateur avant la coupure du secteur GENERATEURS CONTROLE DE TEMPERATURE Cette fonction permet une r gulation automatique de la temp rature du gel par l interm diaire d une sonde thermique appliqu e sur la plaque de verre ext rieure du s quenceur Display 1 IL suffit de s lectionner La fonction C sur le g n rateur Cette Volt mA Watt fonction remplace la r gulation de puissance S lectionner la e Integrateur Minuterie pour arr t automatique temp rature maximum du gel ne pas d passer durant la migration Temp rature de consigne La temp rature du gel sera ainsi maintenue une valeur stable durant la migration En effet si la temp rature de consigne est atteinte en cours de migration le param tre r gul v
79. e des chantillons Largeur des dents 330101 Peigne 1 0 mm 64 ul 16 0 mm 330102 Peigne 2 0 mm 128 ul 16 0 mm 330103 Peigne 3 0 mm 192 ul 16 0 mm 330201 Peigne 1 0 mm 26 ul 6 5 mm 330202 Peigne 2 0 mm 52 ul 6 5 mm 330203 Peigne 3 0 mm 78 ul 6 5 mm 330301 Peigne 1 0 mm 16 ul 4 0 mm 330302 Peigne 2 0 mm 32 ul 4 0 mm 330303 Peigne 3 0 mm 48 ul 4 0 mm 334421 Double peigne 1 0 mm m 14 24 ul 3 5 6 mm 334422 Double peigne 2 0 mm 44 21 28 48 ul 3 5 6 mm 330412 Peigne pr paratif 1 0 mm 1 chantillon 1 marqueur 360 pU 2 x 12 ul 90 0 3 mm 330414 Peigne pr paratif 1 0 mm 2 chantillons 2 marqueurs 160 pl 2 x 12 ul 40 0 3 mm 331100 Portoir transparent aux U V 20 x 24 cm l x L avec 2 parois coulissantes pour coulage du gel 331050 Portoir transparent aux U V 20 x 12 cm 1 x L avec 2 parois coulissantes pour coulage du gel 320700 Jeu de cordons d alimentation 340500 Base de coulage de gel avec niveau a bulle et pieds r glables pour 1 gel de 5 mm d paisseur compatible avec pipettes multicanaux CUVES POUR ACIDES NUCLEIQUES E 8 NOMBRE DE PEIGNES ET DIMENSIONS DES GELS 20 cm E e 24 cm 12 cm Portoir R f 331100 Portoir R f 331050 Double peignes R f 334421 et R f 334422 pour 8 peignes pour 2 peignes Les 2 c t s 21 et 44 dents sont compatibles pipettes multicanaux G N RATEURS RECOMMAND S Page 62 PS 304 XL R f 160600 PS 305 XL Ref 170500 CUVES DE TRANSFERT CONSEILL ES Pages 98
80. e visibility and make size gel running MINIGEL 2 and sample loading easier MICROGEL 2 have rubber feet to reduce slipping When the lid is open a barrier prevents power leads to be connected to the tank Gold plated connectors and platinium electrodes avoid corrosion and poor electrical contact Multiple comb Slots allow flexible capacities Direct position of the comb the height does not have to be adjusted Removable casting gates fit into grooves at each side of the gel tray to create tape free seals The gel is directly poured in the gel tray outside or inside the tank One set of two removable casting OPTION Separate Gel casting base gates is provided with each gel tray Designed for the full range of gel trays the casting base Cat No 340500 and Cat No 340600 can be separatly provided They are equiped by three adjustable feet and one levelling bubble MICROGEL 2 Gel dimension 5 x 8 wx l 2 comb Slots APPLICATION Designed for very fast Separations and small scale procedures of nucleic acids restriction fragments in agarose gel Compact size Whole unit can be placed on U V table Buffer Volume 150 ml Maximum number of samples 20 Unit dimensions wx lx h 19x11x6cm ONE FREE GEL TRAY WHEN ORDERING 2 ADDITIONAL COMBS Includes Removable U V transparent gel tray 5 x 8 cm w x l 2 removable casting gates 1 0 mm thickness 10 teeth comb sample volume10 ul safety lid set of po
81. ed ranges are given on the web site Low concentration gels should be run in the cold room they are fragile and allow more sample diffusion High concentration gels are friable crumble easily and will not allow large DNA molecules to enter the gel 2 Gelpreparation Agarose should be boiled only long enough to dissolve all crystals Overboiling will weaken the matrix Cool to 60 C before pouring to avoid damage to the gel mold Allow the gel to cool gradually after pouring rapid cooling will trap swirling and convection currents in the set gel which can distort bands which pass through them NOTE ON ALKALINE GELS alkaline gel buffers will hydrolyze hot agarose solutions Boil agarose for alkaline gels in water cool and then add alkaline buffer concentrate just before pouring the gel 3 Wells Use care in removing the comb after casting check to be sure that the gel is completely cooled Twisting of the comb on removal will tear the walls of the well allowing sample to seep into the tear and generating distorted band shapes Rapid removal of the comb will create a vacuum which will tear out the bottom of the wells allowing sample to seep out before it can enter the gel Al ImmobilizecLcharges Poor grades of agarose can carry sulfate groups which under electrophoresis will generate a reverse flow of water electroendosmosis and which will also interact with the sample Both effects lead to band broadening Use only fresh high qua
82. embled the glass sandwich is placed in the casting system and the lid is mounted to the top pf the casting system The gel can now be cast through the opening in the lid Once the gel has polymerezed the glass sandwiches can be removed from the casting system placed in the gel capture unit and set into the buffer tank Quick and simple Pre Cast gels Using the same gel capture unit 8 cm x 10 cm precast polyacrylamide gels can be inserted locked in place and set into he buffer tank The MaeGEL In will accommodate various brands of pre cast gel The 920000 is an all inclusive easy to use protein electrophoresis system designed for years of continuous use Discover what Ma GEL In can do for you The tank holds a minimum of 350 ml of buffer For longer runs the tank can be filled to a maximm of 850 A stir bar be placed in the tank to recirculate the buffer to keep the ionic concentration constant The tank includes a patented vented lid to further reduce heat buildup during the gel run TANKS FOR PROTEINS EE 2 ON L gr e el SET UP ORDERING INFORMATION 1 Create glass plate sandwich with spacers and glass slides 2 Place glass sandwich into casting system notched plates Includes Center support buffer tank vented lid to inside casting system three inner and three outer glass pl
83. es lat rales pour bloquer les sandwiches G N RATEUR CONSEILL 8 Placer le module dans la cuve d electrophorese PS 304 XL 300 Volts 400 mA R f 160600 Page 62 9 Verser le volume de tampon dans la cuve 10 Proc der au d p t des chantillons R F RENCES DE COMMANDE 50500 Cordons de Replacement 92010 Plaques de verre avec encoche x 5 92020 Plaques de verre pleines x 5 CARACTERISTIQUES CONSTRUCTION 92025 Espaceurs 0 8 mm 2 11 Connecter le g n rateur et lancer la migration 92040 de migration seule Module de migration ABS thermo forme 92050 Module de miaration seul Module de coulage Polycarbonate thermo form 92060 Couvercle de Replacement e Cuve de migration 3 8 acrylique 92070 Joints de coulage de Remplacement x 2 92071 Molettes de Remplacement x 2 92072 Connecteur de Remplacement M 92100 Module de coulage pour 2 gels SU UTES OO SEAS u ZONE EIRE Dimensions du gel 8 cm H x 10 cm 1 92500 Module pour Transfert e Joints Silicone Extrud 2818 BLOTTING MINI GEL TRANSBLOTTER POUR 1 4 MINI GELS 10 x 10 cm Ces cuves poss dent l ensemble des quipements n cessaires l obtention de transferts reproductibles et de haute qualit Systeme de refroidissement par circulation ext rieure situ e en partie inf rieure Anode en titane platin e et cathode en inox assurent une qualit de transfert uniforme Ces lectrodes so
84. eur Nombre de dents Volume des chantillons Largeur des dents 320101 Peigne 1 0 mm 10 24 ul 6 0 mm 320102 Peigne 2 0 mm 1 48 ul 6 0 mm 0 320103 Peigne 3 0 mm 10 72 ul 6 0 mm 320161 Peigne 1 0 mm 16 12 ul 3 0 mm 320162 Peigne 2 0 mm 24 ul 3 0 mm 16 320121 Peigne pr paratif 1 0 mm 1 chantillon 2 marqueurs 360 pl 2 x 12 pl 90 0 3 mm 320700 Jeu de cordons d alimentation 320400 Base de coulage de gel avec niveau a bulle et pieds r glables pour un gel de 5 mm d paisseur compatible avec pipettes multicanaux PACK R f 3110PA COMPREND MINIGEL 2 PS 304 XL UN PORTOIR GRATUIT POUR L ACHAT DE DEUX PEIGNES SUPPL MENTAIRES dd t e m lt ilz t L HH 0 Page 62 5 BT UI acnes NUCLEIQUES Ih MIDIGEL 2 APPLICATION Cuve recommand e pour des gels pr paratifs ou analytiques sur gels de tailles moyennes Convient egalement pour la separation des ADN et ARN RFLP screening des PCR Nombre maximum d chantillons 72 avec 4 peignes e Portoir 10 x 14 cm recevant 4 peignes Portoir 10 x 8 cm recevant 2 peignes e Peignes conventionnels et modele 18 dents compatibles pipettes multicanaux e Peigne pr paratifs e 4 pieds reglables et niveau a bulle e Bandes rouges pour meilleure visualisation pendant le d p t des chantillons e Ports pour circulation de tampon UN PORTOIR GRATUIT POUR LACHAT DE DEUX PEIGNES SUPPLEMENTAIRES CAR
85. f 1mm thick gels in 30 minutes at 24 Volts THE BEST SPECIFICATIONS OF THE SLIM BLOTTERS e LOW BUFFER VOLUME e No maintenance on the platinized Titanium electrodes e Standard alloy electrodes should be wiped occasionally Just a few minutes for loading e High surface area assures a high field at the gel e 5 models with alloy electrodes for WESTERN Blot e 5 models with platinized Titanium anode for SOUTHERN and NOTHERN blotting e No bubble surrounding the electrodes ORDERING INFORMATION 504001 10 x 15 cm unit with alloy anode for Western Blots Buffer 350 ml 504015 15x 20 cm unit with alloy anode for Western Blots Buffer 800 ml 504020 20x24 cm unit with alloy anode for Western Blots Buffer 1400 ml 504040 25x 34 cm unit with alloy anode for Western Blots Buffer 2500 ml 504002 10x 15 cm unit with Platinized Titanum anode for Southern and Western Blots Buffer 350 ml 504017 15x 20 cm unit with Platinized Titanum anode for Southern and Western Blots Buffer 800 ml 504021 20x 24 cm unit with Platinized Titanum anode for Southern and Western Blots Buffer 1400 ml 504041 25 x 34 cm unit with Platinized Titanum anode for Southern and Western Blots Buffer 2500 ml 504005 10 x 15 cm Platinized Titanum anode for nucleic acid blots 504010 15x20 cm Platinized Titanum anode for nucleic acid blots 504027 20x24 cm Platinized Titanum anode for nucleic acid blots 504044 25x34 cm Platinized Titanum anode for nucleic acid blots
86. fers amp Solutions Troubleshooting Guide Usual Information for Electrophoresis Version francaise Genetic Code Poster 4 13 6 7 8 9 10 11 10 11 12 13 14 27 16 17 18 19 20 21 22 23 24 25 26 27 28 41 30 31 32 33 34 35 36 37 38 39 40 41 42 45 42 43 44 45 46 47 48 52 53 54 56 57 58 a 103 104 ee 3 gt 5008 ee 92 ee BR gg PERFORMANCE APELEX electrophoresis power supplies are developped and produced utilizing the highest quality of components and materials according to the worldwide standards and safety regulations APELEX power supplies are equipped with many user friendly design features which allow quick and simple operation SAFETY Our power supplies have been designed according international and CE European standards a strict Quality Control Program ensures the excellence and high performance of all APELEX products QUALITY APELEX warrants these products to be free from defects in materials and workmanship under normal service for 3 years from date of shipment This is your guaranty that each power supply conforms to rigorous quality standards 10 5504 papuswwossy P Dumo1g u19 S29M 5unjojg Aipiwas 615 5196 SIS910Ud01129 3 u 10 d 1990 4255 5 gt 20 04128 80s Buiou nb s pide 319 2nN
87. g or low percentage gels which are fragile at room temperature e Gel overloaded 2 nuclease in samples 1 or excessive salt in samples 0 0 Run parameters not optimal 1 2 buffer mismatch D 1 3 or fixed charges gel 4 Gel heating during run check run parameters 1 2 and buffer 0 3 Nuclease degradation B L gel run too fast C l or buffer mis match D 3 Alkaline buffer hydrolyzed matrix 2 No buffer in gel D 2 Nuclease digestion of samples 1 wrong percentage gel used 1 voltage too high C L or dye overload 3 Buffer Related Problems Buffer problems are by far the most common cause of total failure of gels A buffer imbalance will lead to changes in the voltage current or wattage of the run 1 Sample contains too much salt Salt ions in the sample will migrate as a zone of high conductivity low resistance and low voltage The initial migration of the bands will be altered resulting skewed or broad bands or aberrant band positions High salt samples should be dialyzed or otherwise desalted before loading 2 No buffer in gel Failure to add buffer to the gel creates a high resistance across the gel with no ions to carry the current The tracking dye stacks in the zone just behind the buffer front and migrates slowly into the gel 3 Gel or Tank buffer incorrect A mismatch between gel and tank buffers will lead to a salt
88. gel surface area de surface de gel platin e 10 14 Volts maximum 10 14 Volts max e Un courant de 3 mA cm de gel permet un transfert rapide Temps 30 minutes 2 heures 30 minutes e Syst me de serrage uniforme assurant une pression de migration 2 heures gale entre le gel et la membrane de transfert ID AL POUR DES PROT INES INF RIEURES A 150 KD TRANSFERT DE MINIGELS EN 15 MINUTES CONCUES POUR UNE UTILISATION INTENSIVE Couvercle lourd avec cathode en acier inoxydable Anode en Titane platin sans entretien Base avec molettes de serrage larges et pratiques Petit format pour gels de 11 x 11 cm Grands gels jusqu 20 x 20 cm ou 4 gels 10 x 10 cm CUVES POUR BLOTTING TROIS SOLUTIONS POUR LE TRANSFERT Solution 1 TRANSFERT SEMI SEC Recommand pour le transfert rapide des PROTEINES e Compatible pour les ADN et ARN Ne n cessite pas de volume de tampon significatif Solution 2 TRANSFERT EN MILIEU LIQUIDE GEL TRANSBLOTTEURS e Un seul type de cuve pour tous les transferts WESTERN BLOT PROT INES NORTHERN BLOT ADN SOUTHERN BLOT ARN Solution 3 TRANSFERT EN MILIEU LIQUIDE SLIM BLOTTERS e Faible volume de tampon e Transfert tres rapide e Transfert a faible voltage e 5 cuves pour le Western Blot e D cuves quip es d une anode en titanium avec surface platin e e Transfert DES ADN DES ARN ET DES PROT INES 10 e MINI VERTIGEL 2 Ref
89. h 6 5 mm 36 teeth comb 0 8 mm MC pipette compatible Well volume 34 ul Teeth width 2 7 mm Set of 2 x 0 8 mm spacers Plain glass plate 20 x 10 cm W x H 1 piece Notched glass plate 20 x 10 cm W x 1 piece Water cooled removable Running module already included in the package Cat 410000 Replacement clamps for running module Cat No 410010 2 Replacement gasket for running module Cat No 410010 x 2 Gel casting base already included in the package Cat No 410000 Replacement gasket for casting base Cat No 410020 2 Gel casting base running module H VERTIGEL 2 fully equipped for 2 x 1 5 mm gel 20 x 20 cm gel casting base Includes 2 notched glass plates 4 plain glass plates 1 blind plate 2 x 15 wells 1 5 mm combs 2 x 20 wells 1 5 mm combs 4 spacers 1 5 mm 2 replacement gasket internal running module with internal cooling chamber safety lid with power cords gel casting base user s manual 10 teeth comb 1 5 mm Well volume 449 ul Teeth wide 13 mm 15 teeth comb 1 5 mm Well volume 271 pl Teeth wide 8 mm 20 teeth comb 1 5 mm Well volume 182 pl Teeth wide 5 5 mm 25 teeth comb 1 5 mm Well volume 129 ul Teeth wide A mm Prep comb 2 teeth 1 5 mm Well volume 4230 pl 135 pl Teeth wide 151 5 mm Set of 2x 1 5 mm spacers 10 teeth comb 0 8 mm Well volume 239 ul Teeth wide 13 mm 15 teeth comb 0 8 mm Well volume 144 ul Teeth wide 8 mm 20 teeth comb 0 8 mm Well volume 97 ul Teeth wid
90. he gels are of uniform thickness providing an even sample migration and minimum lane distortion Gel can be cast in the inner running frame The MINI VERTIGEL 2 is designed order to be compatible with our pre cast gels and with most pre cast gels 10 x 10 cm Cooling is a built in equipment but not necessary for all applications All VERTIGEL models have a gel casting system using the slab gel running module and a leak free separate casting base equipped by two silicone seals and two clamping cams This system allows two gels to be cast at a time directly in the gel running module Then after gel polymerisation the running module is removed from the casting base and directly placed the outer buffer tank e Easy to use clamping system e Safety lid with integral power leads e Long life pure platinum electrodes are both protected and user replaceable e Gold plated terminals for corrosion resistance eak free buffer seals e Float glass plates ensure uniform gel thickness and straight sample migration e Supplied with casting base Complemented with a full range of combs and spacers TANKS FOR PROTEINS THE BEST FEATURES OF THE TWIN VERTIGEL SYSTEMS Integral Running cooling module serves chamber as casting stands 4 Low buffer volume Easy to clean easy to remove bubbles between gels and buffer 7 4 oser D 1 F12 ED 7 YK
91. hort circuit open circuit or sudden overload Constant voltage or constant current with Automatic restart in case of power failure When the automatic crossover between parameters power returns an audible alarm rings for 10 seconds then the START mode turns on automatically at the e Two red LEDs indicate the constant parameter preset values owes SUPPLIES POWERFUL EASY TO USE AND ROBUST PS 202 For Botting Cat No 176000 200 Volts e 1998 mA PS 608 Cat No 173000 600 Volts e 800 mA PS 1006 Cor proteins Cat No 174000 1000 Volts e 600 mA EASY T0 USE PS 1006 Electrophoresis Power Supply 1 DANGER HIGH VOLTAGE Timer POWER SUPPLIES ALL POWER SUPPLIES FEATURES Last settings are automatically restored at power up Tactile membrane panel for precise easy input of parameters Easy to clean Constant voltage or constant current with automatic crossover between parameters Two red LEDs indicate the constant parameter The units be operated in timed mode and will automatically terminate output Continuous mode may also be operated It is possible to perform electrophoresis with constant current whilst limiting the voltage to a selected value or with constant voltage whilst limiting the current to a selected value If one of the non controlling output set values 15 reached during the run the power supply will automatically crossover an
92. hylene bisacrylamide Also designed for Protein separation ANAMED ANAMED PRECAST GELS The following gels are manufactured in a 10 cm x 10 cm one way use plastic cassette and fit into the Mini Vertigel 2 tank see page 30 and most of the commercial available electrophoresis minicells such as Novex Serva Hoefer e g The gels are available in packs of ten per gel type no single gels APELEX anamed precast gel PAG are manufactured to be native gels Therefore they fit perfectly for to use in native or SDS Electrophoresis All Laemmli gels have a 4 stacking gel APELEX ANAMED precast gel Mini Vertigel 2 PC Cat No 400800 A reliable standard directly from the refrigerator Designed for precast gels 10 x 10 cm Supplied with Our specialists have several years of first hand Running Module No other accessoiries for Casting Gels experience In the development and production of precast gels We submit our products to a strict See page 40 the detail of additionnal accessories as qualit control with a certificate of analysis GLP combs spacers glace plates and further items VarioGel the cleaver Solution for your Protein or DNA Electrophoresis IDEAL IN PERFORMANCE FOR NARROW MOLECULAR WEIGHT RANGES VG 41210 4 12 VarioGel 9 1 mm 10 wells 10 pieces VG 41215 4 12 VarioGel 9 1 mm 15 wells 10 pieces BUFFER SOLUTIONS FOR ELEKTROPHORESIS WITH VARIOGEL VM 05509 MOPS Tris SD
93. ickness 1 mm compatible with multichannel pipettes 340600 Gel Casting base with adjustable feet and bubble designed to hold gel tray 23 x 40 cm and 23 x 25 cm 811270 Safety lid with attached power leads 810280 Accessories for buffer circulation 8100PH PACK H Includes Power Supply PS 608 MAXIGEL XXL equipped as follow 8112340 Removable U V transparent gel tray 23 x 40 cm w x l for up to 12 combs 8102325 Removable U V transparent gel tray 23 x 25 cm w x for up to 10 combs 8101242 8 x Combs 26 teeth 1 mm 24 2 markers compatible with multichannel pipettes 8125SLG Sample Loading guide for 26 wells Comb 340600 Gel Casting base with adjustable feet and bubble designed to hold gel tray 23 x 40 cm and 23 x 25 cm 173000 Power Supply PS 608 Thickness Teeth Sample volume Well width 810125 Comb 1 0 mm 16 ul 7 2mm 810150 Comb 1 0 mm 7 ul 3 0 mm 8101242 Comb 1 0 mm 26 24 2 ie 16 7 ul 7 2 3 0 mm 811525 Comb 1 5mm 24 ul 7 2mm 811550 Comb 1 5mm 10 ul 3 0 mm 8115242 Comb 1 5mm 26 24 2 ai 24 10 ul 7 2 3 0 mm 812025 Comb 2 mm 32 ul 7 2mm 812050 Comb 2 mm 14 ul 3 0 mm for 5 mm thickness gel A JIS POLYACRYLAMIDE 6215 Our VERTIGEL electrophoresis units are manufactured to a high specification and design to combine versatility with safety The robust gel tanks are easy to use and are offering many gel sizes standard gels All VERTIGEL gel units are suppplied with float glass plates to ensure that t
94. ignes a Pas de r glage P c e peee positionnement K imm diat Deux parois coulissantes s inserent de chaque c t afin d obturer les ouvertures oppos es Le gel peut tre directement coul en dehors de la cuve Apr s polym risation retirer avec pr caution OPTION Bases de coulage de gel ind pendantes les peignes et les deux parois coulissantes Compatibles avec tous les portoirs de la gamme les bases de coulage R f 340500 et R f 340600 sont fournies s par ment Elles sont quip es de pieds r glables et d un niveau bulle MICROGEL 2 E Dimensions du gel 5 x 8 cm l x L Recoit 2 peignes APPLICATION S parations rapides d acides nucl iques RFLP contr le de PCR d un petit nombre d chantillons Convient galement pour l enseignement Dimensions r duites La cuve est compl tement transparente aux U V Pas de n cessit de sortir le portoir la cuve compl te est plac e sur la table U V Volume de tampon 150 ml Nombre maximum d chantillons 20 Dimensions de la cuve Lx p x h 19 x 11 x 6 cm UN PORTOIR GRATUIT POUR L ACHAT DE DEUX PEIGNES SUPPL MENTAIRES Fournie avec Portoir transparent aux U V 5 x 8 cm Lx L 2 parois coulissantes peigne 10 dents 1 0 mm d paisseur volume des chantillons 10 pl couvercle paire de cordons d alimentation notice d emploi paisseur Nombre de dents Volume des chantillons Largeur des dents 300052 Peigne 2 0 mm 5
95. ino acid By following the color coded section outward you will find structural information and related post translational modifications e e e b b S8 P 9 9 9 c c9 c RW WWW BP B GO 0 D b ne o e 0 O O O O O O O O 9 9 9 9 9 9 9 9 WWW O e O O O O O O 0 9 9 9 9 9 9 Q s eegen 0e 00 0 0 O O O 5B 9 O O9 6 5B 9 B P 9 amp 9 k c c c O0 O O O O O O O O 9 9 9 9 b 9 9 geg sg h eoo 50 00 58 B O95 DO 8 JB F 9 P se O 000 0 O O O O O O O 69 9 5 59 9 9 4 b bn ee oo000 0 009 WW WWW O9 9 9 0 5 9 5 oo00 0 0 00 O O O O OG O9 9 9 9 9 9 9 b ee 000 00 0 0 0 0 0 O O 9 9 0 9 ee 1o0000 0 0 0 O O O O O O 9 9 9 9 amp n eo 000000 0 0 0 5 B 0 9 9 5 5 Xe PLL 0 0 0 0 0000000070 8 J X X Oo 00 0 0 06 0 0 5 0 9 e 5 5 er a 0 000 0 0 0 8 08 09 0 5 8 a Ce ee ee 0 0 6 0 O O O9 9 9 0 b ege RSR i 0oo000 00 0 0 O O6 O0 06 9 w a a eS De Dee Da Daa De Ze Za 0 0 2 0 s e e e e WWW OO ee 0 5 0 5 0 5 O 6 e 5 s s EH E NH NM X X e O 9 n a e see w w NM Peto 0 0 ee e o o o 6 6 B
96. leurs programm es Volts mA et Watts SP CIFICATIONS 10 3000 volts 15 300 mA 0 3 300 watts Gamme de fonctionnement Puissance maximum 300 watts Valeur minimum r gul e 10 volts 1 mA 1 watt 0 3000 volts Pas de 10 v 0 300 mA Pas de 1 mA 0 300 watts Pas de 1 watt Affichage et r glage r solution D bit Minimum 10 volts 15 pA 0 3 W D tection de fautes stop et alarme sonore Temp rature de fonction 0 C 40 C Dimensions 27 x 34x 11 cm Lx p xh Poids 4 kg LES PACKS DES SOLUTIONS CONOMIQUES AVEC NOS PACKS POUR ELECTROPHORESE DES ADN ARN SUR AGAROSE PS 305 XL MAXIGEL XXL R F RENCES PACK A R f 3120PA Cuve MINIGEL 2 R f 311000 ar G n rateur PS 304 XL R f 160600 PACK 96S R f 372696 Cuve MIDIGEL XL 96 chantil R f 371000 ar G n rateur PS 304 XL R f 160600 PACK B R f 3760PB Cuve MIDIGEL XL R f 370000 ar G n rateur PS 304 XL R f 160600 PACK C R f 3420PC Cuve MAXIGEL ECO 2 R f 341000 G n rateur PS 305 XL Ref 170500 PACK Ref 3220PF Cuve MIDIGEL 2 Ref 321000 ar G n rateur PS 304 XL Ref 160600 PACK Ref 3320PG Cuve MAXIGEL THERMO 2 Ref 331000 ar G n rateur PS 305 XL Ref 170500 PACK H Ref 8100PH Cuve MAXIGEL XXL Ref 812340 ar G n rateur PS 608 Ref 173000 d Il UUR GELS D AGAROSE IMMERGES SOLIDITE Les Cuves Couvercles et Portoirs pour gels sont usin s dans de l acrylique 10 ou 6 mm
97. lity Agarose with an EEO specification of 0 15 TroubleShooting Agarose DNA Gels Symptom D Sample Preparation Degradation of samples leads to smeared or doubled bands Overloading will cause bands to smear and will alter band positions 1 2 3 Nuclease degradation Exonu cleases will cause bands to smear down endonucleases will generate new bands Keep samples cold include EDTA where practical Overloading Loading of more than 10 ug lane of DNA will cause upward smearing of the bands Dye overload In some cases bands will comigrate with the tracking dye The dye will absorb the ethidium fluorescence during staining obscuring the band Soaking the gel in water or buffer for 1 hour will allow the dye to diffuse enough to see the bands Diagnosis normally rp band at the top of the gel Run Conditions The voltage and temperature maintained during the run can have marked effects on the results 1 2 Electrical parameters Most gels are meant to be run at 5 15V cm Running at too low a voltage too slow allows the bands to diffuse too much before the run gag Is finished Too high a voltage causes heating which can melt the gel Increasing the voltage also selectively increases the mobility of larger DNA molecules compressing the band pattern Temperature In general gels should be run as cold as is convenient This is particularly true for low meltin
98. lized on Membranes Immunological Detection of Proteins Enzyme Linked Immunosorbent Assay ELISA Western Blotting Troubleshooting Denaturing DNA PAGE Gels page 54 Troubleshooting Agarose DNA Gels page 55 Troubleshooting Denaturing Protein Gels page 56 Useful Information for Electrophoresis page 57 poo lt lt Ew ZA nglish Contents Listing of Applications you can get on our Web Site www apelex fr Selection Guide for Tanks and Power Supplies Power Supplies e PS 304 XL PS 305 XL PS 503 XL e PS 202 PS 608 PS 1006 e Programmable H V Unit for IEF SSCP Sequencing PS 9009 TX e Programmable Units PS 9009 TX PS 1006 P e High Voltage Unit 3000 volts PS 3003 Packs for Nucleic Acids Electrophoresis Tanks for Nucleic Acids Electrophoresis e MICROGEL 2 e MINIGEL 2 e MIDIGEL 2 e MIDIGEL XL e MAXIGEL ECO 2 e MAXIGEL THERMO 2 e MAXIGEL XL MAXIGEL XXL Tanks for Proteins Electrophoresis Systems for SDS and Pre Cast gels e MINI VERTIGEL 2 for 10 x 10 cm gels e MINI WIDE VERTIGEL 2 for 20 x 10 cm gels e MIDI VERTIGEL 2 for 16 x 18 cm gels e H VERTIGEL 2 for 20 x 20 cm gels e Accessories for Vertigel tanks e MaeGeleln Low Cost Tank Tanks for Blotting e Liquid Electro Blotting e Semi Dry Electro Blotting e FAQ about Electro Blotting Packs for Protein Electrophoresis and Electro Blotting Pre Mixed gels amp Solutions Pre Cast gels Buf
99. lute tank buffer will lead to a salt discontinuity which migrates through the gel distorting the band pattern with a wave effect 4 Gelbuffer incorrect The gel provides most of the resistance to current flow changes in the gel buffer will alter the electrical parameters of the run Too concentrated a gel buffer will allow 2 Pre Run Gels must be pre run for more current to flow leading to more heat generation with distortions such as smiling 30 min to warm the gel to operating Too dilute a gel buffer will increase resistance to current flow which may actually sharpen the temperature Failure to pre run bands but generally slows the run and reduces resolution gives doublet bands 5 Glycerol containing samples Glycerol forms complexes with the borate in TBE buffer creating 3 Heat exchange Heat must be a zone of low conductivity which migrates slowly through the gel creating a wave pattern conducted away from the gel to avoid thermal gradients in the gel which cause smiling or other pattern distortions Use a non glycerol loading buffer or run gels in a borate free buffer such as TTE WWW APELEX FR D Matrix Related Problems Problems with the matrix will manifest as smeared bands and or loss of resolution Note that most total failures of electrophoresis are not matrix related see D Buffer related problems 1 Pore size This is determined by the concentration and type of agarose used Suggest
100. lution 2 No buffer in gel D 2 Protease digestion of samples B l or wrong pore size used 1 D Buffer Related Problems Buffer problems are by far the most common cause of total failure of gels A buffer imbalance will lead to changes in the voltage current or wattage of the run 1 2 3 4 Sample contains too much salt Salt ions the sample will migrate as a zone of high conductivity low resistance and low voltage Until this zone migrates away from the sample the migration of the protein bands will be altered resulting skewed or broad bands or aberrant band positions High salt samples should be dialyzed or otherwise desalted before loading No buffer in gel Failure to add buffer to the gel creates a high resistance across the gel with no ions to carry the current The tracking dye stacks in the zone just behind the buffer front and migrates slowly into the gel Tank buffer incorrect The Laemmli buffer system uses a buffer discontinuity to stack the Samples prior to separation as a result it is more tolerant of minor buffer changes than a continuous system Use of too concentrated or too dilute tank buffer will lead to a salt discontinuity which migrates through the gel distorting the band pattern with a wave effect Gel buffer incorrect The gel provides most of the resistance to current flow changes in the gel buffer will alter the electrical parameters of the run Too concentrate
101. lysis and quick screening of all samples from 3 x 96 position PCR thermal cycler on one gel restriction fragments screening e Long length separation 12 cm or 24 cm according the selected Gel Tray e Maximum number of samples 352 using 8 combs Cat No 334421 or Cat No 334422 compatible with multichannel pipettes e Gel tray 20 x 24 cm has 8 spaces for comb insertion Y e Gel tray 20 x 12 cm has 2 spaces for combs insertion e Combs 10 20 30 teeth and preparative models ONE FREE GEL TRAY WHEN ORDERING 2 ADDITIONAL COMBS Double combs 44 21 teeth compatible with multichannel pipettes TECHNICAL SPECIFICATIONS e 4 adjustable feet and bubble level Buffer Volume 1 800 ml e Red strips for wells vizualisation Unit dimensions e Ports for buffer circulation w x Lx h 28 x 37 x 9 cm ORDERING INFORMATION 341000 MAXIGEL ECO 2 Includes Removable U V transparent gel tray 20 x 24 cm w x 2 removable casting gates 1 0 mm thickness double sided comb 44 21 teeth sample volume 14 24 ul Cat No 334421 Compatible with multichannel pipettes safety lid set of power leads buffer circulation accessories user s manual 3410PC PACK C Includes MAXIGEL ECO 2 Fully equipped as above Cat No 341000 Power supply PS 305 XL Thickness Teeth Sample volume Well width 330101 Comb 1 0 mm 64 ul 16 0 mm 330102 Comb 2 0 mm 128 ul 16 0 mm 330103 Comb 3 0 mm 192 ul 16 0 mm 330202 Comb 2 0 mm 52 ul 6 5 mm 330203 Comb 3 0 mm
102. mb 2 0 mm 24 ul 3 0 mm 320163 Comb 3 0 mm 16 36 ul 3 0 mm 320181 Comb 1 0 mm 18 14 pl 3 5mm compatible with multichannel pipettes 320121 Prep Comb 1 0 mm 1 sample 2 markers 360 pU 2 x 12 ul 90 0 3 mm 320122 Prep Comb 2 0 mm 1 sample 2 markers 720 uU 2 x 24 pl 90 0 3 mm 321050 U V transparent tray 10 x 8 cm w x l Supplied with 2 removable casting gates 320700 Set of power leads 340500 Separate gel Casting base with adjustable feet and bubble for a b mm thickness gel PACK A Cat No 3110PA INCLUDES MINIGEL 2 PS 304 XL ONE FREE GEL TRAY WHEN ORDERING 2 ADDITIONAL COMBS APELEX PS 304 XL 999 e O Page 6 IT BEN Heel NUCLEIC ACIDS MIDIGEL 2 APPLICATION Accomodates standard gels for both analytical and preparative studies of nucleic acid Designed for separations of DNA and RNA restriction fragments in agarose gel as well as PCR9 screening and RFLP e Maximum number of samples 72 with 4 combs e Gel tray 10 x 14 cm has 4 spaces for combs insertion e Gel tray 10 x 8cm has 2 spaces for combs insertion e Combs 10 16 and 18 teeth compatible with multichannel pipettes e Preparative combs also available La i 4 adjustable feet and bubble level o Ko t e Red strips for wells vizualisation ONE FREE GEL TRAY WHEN ORDERING 2 ADDITIONAL COMBS TECHNICAL SPECIFICATIONS Buffer Volume 800 ml Unit dimensions w xl x h 28 x 16 x 9 cm ORDERING INFORMATION 3
103. ment notice Peigne 10 dents 1 5 mm volume des puits 449 ul Largeur des dents 13 mm Peigne 15 dents 1 5 mm volume des puits 271 ul Largeur des dents 8 mm Peigne 20 dents 1 5 mm volume des puits 182 ul Largeur des dents 5 5 mm Peigne 25 dents 1 5 mm volume des puits 129 ul Largeur des dents 4 mm Espaceurs 1 5 mm x 2 Peigne 10 dents 0 8mm volume des puits 239 pl Largeur des dents 13 mm Peigne 15 dents 0 8 mm volume des puits 144 ul Largeur des dents 8 mm Peigne 20 dents 0 8 mm volume des puits 97 pl Largeur des dents 5 5 mm Peigne 25 dents 0 8 mm volume des puits 69 ul Largeur des dents 4 mm Espaceurs 0 8 mm x 2 Plaque de verre pleine 20 x 10 cm x 1 Plaque de verre avec encoche 20 x 10 cm x 1 Paroi d obturation pour migration d un seul gel Module de s paration avec lectrodes fourni avec la cuve R f 410000 Pinces de rechange pour module R f 410010 x 2 Joints de rechange pour module R f 410010 x 2 Base de coulage de gel d j inclus dans la r f rence R f 410000 Sachet de 2 joints pour syst me de coulage de gel R f 410020 Base de coulage de gel module de s paration H Vertigel 2 compl te pour 2 gels 19 x 18 5 cm 1 paisseur 1 5 mm base de coulage de gels et joints de remplacement 4 plaques de verre avec encoche 4 plaques de verre pleines 20 x 20 cm 1 paroi d obturation 2 peignes 15 dents 1 5 mm 2 peignes 20 dents 1 5 mm 4 espaceurs 0 8 mm couvercle ave
104. n 1 or insufficient pre run C 2 Ammonium Persulfate or TEMED too old 2 or too much oxygen in the gel solution A 2 sha rp band at the top of the gel No buffer in gel D 2 Nuclease digestion of samples B l or wrong pore size used 1 Run Conditions D Buffer Related Problems The voltage and temperature maintained during the run can have marked effects on the results 1 Electrical parameters run gels at constant wattage 45 55 watts to maintain the temperature above 50 C which keeps the sample denatured Too low a wattage allows the gel to cool to the point that the samples will begin to renature Running at too high a voltage causes smearing and or smiling Buffer problems are by far the most common cause of total failure of gels A buffer imbalance will lead to changes in the voltage current or wattage of the run 1 Sample contains too much salt Salt ions in the sample will migrate as a zone of high conductivity low resistance and low voltage The initial migration of the sample bands will be altered resulting in skewed or broad bands or aberrant band positions High salt samples should be dialyzed or otherwise desalted before loading 2 Nobufferin gel This creates a high resistance across the gel With no ions to carry the current the tracking dye stacks in the zone behind the slow moving buffer front 3 Tank buffer incorrect Use of too concentrated or too di
105. n broader bands 2 Polymerization Gels which take longer than usual to polymerize will generally give broad and distorted bands due to local variations in gel quality Polymerization of the gel is a chain reaction initiated by Ammonium Persulfate APS and TEMED The extension of initiated chains is Inhibited by oxygen APS and TEMED must be fresh for consistent results APS should crackle when dissolving TEMED should be clear with no yellow color Use of old reagents will lead to Incomplete polymerization which 15 further inhibited by low levels of dissolved oxygen Degassing will remove Oy giving better polymerization but it is not a substitute for fresh reagents Cold temperatures will also slow polymerization cast gels at room temperature 3 Well Formation sharks tooth comb The bottom of the well must be smooth and flat Remove the comb after casting slowly under buffer Reinsert the comb carefully so as not to over penetrate the gel surface Do not withdraw the comb once inserted well distortions and leakage will result Al Immobilized charges Acrylamide is subject to oxidation producing acrylic acid The immobi lization of acrylic acid in the matrix generates a reverse flow of water electroendosmosis and allows the proteins to Interact with the matrix which leads to band broadening Use only fresh acrylamide solutions within their shelf life which have been properly stored TroubleShooting Denaturing DNA PAGE Gel
106. nd DNA Fragments PCR Screening Protein Electrophoresis 2 D Electrophoresis Preparative gel SDS Page Semidry Blotting Western Blotting E Recommended Possible PS 1006 MES SUPPLIES PROGRAMMABLE PS 9009 TX Cat No 119030 3000 Volts e 300 mA 300 Watts DANGER HIGH VOLTAGE PS 1006 P WE Cat No 119050 1000 Volts 600 mA 300 Watts DANGER HIGH VOLTAGE FEATURES Memory capable of storing e 16 one step programs with automatic cut off e 8 sequenced programs with up to 10 steps which automatically follow preset values for each step 1 specific program for low current 15 pA IEF LCD display with 2 lines of 20 characters Twenty switch keyboard for monitoring each function Four operating modes Constant voltage constant current constant power or temperature limitation It is possible to perform electrophoresis with a constant parameter whilst limiting the others to selected values including temperature Automatic crossover from one mode to another when output limits are reached Flashing display indicates the constant mode TEMPERATURE CONTROLLED eoe 0220888240 2 3800008006 TEMPERATURE CONTROLLED 3000 Volts 6 02 x 2 2200060006 POWER SUPPLY Automatic RESTART case of power failure during a cycle When the power returns an audible safety alarm rings for 10 seconds then the S
107. nt 1 en Titane Platin pour Southern et Western Blots Volume de tampon 1400 ml 504041 SLIM BLOTTER avec lectrodes 25 x 34 cm dont 1 en Titane Platin pour Southern Northern et Western Blots Volume de tampon 2 500 ml 504005 Anode seule 10 x 15 cm Titane platin 504010 Anode seule 15 x 20 cm en Titane platin 504027 Anode seule 20 x 24 cm en Titane platin 504044 Anode seule 25 x 34 cm en Titane platin Ten Minute Western Blot ee tm e mte e e 2940 to 20500 Markers Amido Black Stained A 30 Minute Southern Blot HRacdoorap Position de chargement GENERATEURS CONSEILLES PS 305 XL 300 Volts 500 mA R f 170500 PS 304 XL 300 Volts 400 mA R f 160600 Position de transfert 2818 BLOTTING TRANSFERT SEMI SEC 450000 Cuve de Transfert Semi sec petit mod le Proteines ADN ARN Electrodes 11 x 11 cm Membrane Nitrocellulose 460000 Cuve de Transfert Semi sec grand mod le 0 45 um ou 0 2 um Nylon Electrodes 20 x 20 cm PVDF 0 45 um ou 0 2 pm Tampon Tampon Towbin 3 Buffer 0 5X 1XTBE Cuves de transfert Semi sec de Transfert System Bjerrum NAQ petit et grand modeles Schafer Nielsen 2 dimensions 11 x 11 cm amp 20 x 20 cm Transfert Courant Constant Courant Constant e Tr s haute conductivit et long vit des lectrodes 0 8 3 mA cm 0 5 3 mA cm e Cathode en acier Inox anode en titane avec surface
108. nt prot g es et mont es sur des supports amovibles Les cassettes recevant les sandwiches se glissent dans des rainures usin es sur les c t s de la cuve et permettent un serrage troit et reproductible sans risque de d formation pendant le transfert REFERENCES DE COMMANDE 430000 Cuve de Blotting pour 4 gels 10 x 10 cm CENIERATEUR CONSEILL Page 62 livr e avec 4 cassettes de compression et 8 feuilles abrasives ACCESSOIRES 431010 Cassette de compression 431PAD Feuilles abrasives 10 x 10 cm x 4 CARACTERISTIQUES Volume maximum de tampon 1300 ml Dimensions de la cuve H x L x P 15x 13x15cm Surface de transfert 10 x 10 cm Temps de transfert 4 heures Overnight PS 305 XL R f 170500 LARGE GEL TRANSBLOTTER R F 440000 2 GELS 20 x 20 cm GENERATEURS CONSEILLES Page 64 REFERENCES DE COMMANDE PS 202 Ref 176000 ou PS 608 Ref 173000 440000 Cuve de Blotting pour 2 gels 20 x 20 cm livr e avec 2 cassettes de compression 4 feuilles abrasives Volume maximum de tampon 3000 ml Dimensions de la cuve H x L x P 25 x 23 x 10 cm Temps de transfert 4 heures Overnight CUVES POUR BLOTTING LES MODELES SLIM BLOTTER Utilisable pour tous les transferts WESTERN BLOT TRANSFERT DES PROTEINES S lectionner les cuves standards avec lectrodes en acier Inox Transfert partir d un gel de 0 8 mm d paisseur de prot ines jusqu 170 kD en 40 minutes 24 Volts SOUTHERN BLOT TRANSFE
109. oGel P 25 x Concentrate TRIS TRICINE APPLICATIONS Tris Tricine SDS Tris Tricine SDS Tris Tricine SDS Running Buffer 10 x Concentrate Running Buffer 10 x Concentrate Running Buffer 10 x Concentrate Tris Tricine SDS Sample Buffer 2 x Concentrate Tris Tricine SDS Sample Buffer 2 x Concentrate Transfer buffer for ProGel ProGel P 25 x Concentrate Transfer buffer for ProGel ProGel P 25 x Concentrate TRIS BORATE EDTA TBE BUFFERSYSTEM FOR NUCLEIC ACID ELECTROPHORESIS TBE Running buffer 5x Concentrate TBE Running buffer 5 x Concentrate TBE Sample buffer 6 x Concentrate TBE Sample buffer 6 x Concentrate TBE Sample buffer 5 x Concentrate extra heavy TBE Urea Sample Buffer 2 x Concentrate TBE Urea prep Sample Buffer 2 x Concentrate MARKER AND STANDARDS ProteMix Proteinstandard Blue Prestained Proteinmarker with 12 Components 220 2 5 kDa with 8 Components 175 6 5 kDa GEL STAINING AND DESTAINING AzurGel K Colloidal Coomassie Kit contents of 2 solutions and an instruction sheet sufficient for 25 anamed minigels AzurGel Super ready to use staining solution sufficient for approx 50 anamed minigels ArgentQuick Silverstaining Kit for detection of traces 5 solutions ea 125 ml and an instruction sheet sufficient for 25 anamed minigels GEL DRYING AnaFix Geldrying Kit for 10 x 10 cm Gels 2 Drying frames 200 Cellophane sheets and 500 ml AnaRapid AnaFix Geldrying Kit 1
110. oltage courant ou puissance sera automatiquement et proportionnel Display 2 lement diminu afin de controler l chauffement cr e par effet e Volt mA Temp rature de consigne maximum Joules Lorsque la temp rature mesur e par l interm diaire Minuterie d di e l enregistrement du temps de de la sonde redevient inf rieure la valeur de consigne r gulation de temp rature Min les pr r glages initiaux sont de nouveau op rationnels e Minuterie principale pour arr t automatique Ce contr le automatique est constant durant toute la migration SPECIFICATIONS La minuterie principale Min T reste op rationnelle quelles que soient les conditions de r gulation En fin de migration le temps affich sur Min R peut tre utilis pour un compl ment de migration s il d passe 15 minutes L afficheur Min R est d di cette fonction C est une minuterie secondaire qui incr mente le temps coul pendant le contr le de temp rature c est dire le temps pendant lequel les parametres voltage courant ou puissance ont t automatiquement contr l s et baiss s R f 119020 Sonde optionnelle pour contr le de temp rature PS 9009 TX PS 1006 P Gamme de Voltage R solution 10 3000 Volts Pas de 10 Volt 10 1000 Volts Pas de 10 volts Gamme de Courant R solution 15 pA 300 mA Pas de 1 mA 15 pA 600 mA Pas de 1 mA Gamme de Courant avec le Prog 28 0 mA 300 mA
111. on Les cuves VERTIGEL sont fournies completes avec base de coulage de gel pr tes l emploi pour couler et migrer deux gels La MINIVERTIGEL 2 recoit galement les gels pr ts l emploi en cassettes de 10 x 10 cm cet effet elle peut tre command e sous la r f rence R f 400800 et sera livr e sans plaques de verre ni peignes ou espaceurs ni base de coulage e Aucune manipulation des plaques de verre apres Coulage Ge cel e Blocage et serrage facile des plaques de verre e Electrodes en fil de platine croui install es dans des rainures de protection vitant ainsi toute rupture pendant le nettoyage Connecteurs mates et femelles dores evitant la Corrosion e Joints de qualit interdisant toute fuite de tampon entre compartiments e Couvercle avec cables solidaires CUVES POUR PROTEINES LES PLUS DES CUVES VERTIGEL ROBUSTES ET EFFICACES Double corps pour refroidis sement avec un cryostat Le module de migration sert galement de support pour le coulage des gels Faible volume de tampon Pas d lectrode dans le compartiment inf rieur Facile nettoyer Accessibilit id ale pour retirer les bulles sous les S j gels Cryostat Gm L I F12 ED 7 Peignes Teflon La base de coulage Apr s connexion du cryostat pour Espaceurs Teflon associ e avec le refroidissement par circulati
112. on les module central permet parois c ramique du module assurent de couler 1 ou 2 gels une conductivit thermique efficace sans risque de fuites entre le tampon et les gels LA CONCEPTION DES CUVES ET LA QUALIT DES QUIPEMENTS FOURNIS ASSURENT DES MIGRATIONS PARFAITEMENT EXPLOITABLES 0 80 MINI VERTIGEL 2 400000 400800 DESCRIPTION MINI VERTIGEL 2 Livr e complete avec accessoires pour couler et migrer 2 gels de 0 8 mm d paisseur Fournie avec Compartiment inf rieur couvercle avec cordons d alimentation solidaires module de migration quip d un double corps pour refroidissement 2 plaques de verre avec encoche 4 plaques de verre pleines 10 x 10 cm 2 jeux d espaceurs Teflon de 0 8 mm d paisseur 2 peignes Teflon 10 dents 0 8 mm d paisseur volume des chantillons 99 ul 2 plaques thermiques avec encoche 1 plaque d obturation pour migrer 1 gel joints et accessoires de remplacement Base de coulage de gel notice d emploi Recoit galement les gels pr coul s 10 x 10 cm MINI VERTIGEL 2 PC Pour gels pr coul s 10 x 10 cm pages 48 53 Module de migration compartiment inf rieur et couvercle Livr e sans plaques de verre sans peignes ni espaceurs sans base de coulage Gels pr coul s et r actifs H ZZ SS SEE ee PROTEINES Dimensions utiles des gels 9 x 8 5 cm 1 x H e Plaques de verre 10 cm x 8 10 cm Hauteur
113. ox 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 30 200 kDa 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 15 120 kDa 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 6 80 kDa 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 5 60 kDa 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 2 50 kDa 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box 10 pieces in a box lt n wm s s mm ag a lt weg _ lt TG 41210 TG 41212 TG 41215 TG 41250 TG 41252 TG 41255 TG 81610 TG 81612 TG 81615 TG 81650 TG 81652 TG 81655 TG 42010 TG 42012 TG 42015 TG 42050 TG 42052 TG 42055 TG 12010 TG 12012 TG 12015 TG 12050 TG 12052 TG 12055 IDEAL PERFORMANCE BETWEEN 4 12 Tris Gly Gel 1 0 mm 10 well 4 12 Tris Gly Gel 1 0 mm 12 well 4 12 Tris Gly Gel 1 0 mm 15 well 4 12 Tris Gly Gel 1 5 mm 10 well 4 12 Tris Gly Gel 1 5 mm 12 well 4 12 Tris Gly Gel 1 5 mm 15 well IDEAL PERFORMANCE BETWEEN 8 16 Tris Gly Gel 1 0 mm 10
114. r both analytical and preparative studies of nucleic acid Designed for separations of DNA and RNA restriction fragments In agarose gel as well as PCR screening and RFLP e Short and long length separation according the selected Gel Tray e Gel tray 15 x 20 cm has 8 spaces for comb Insertion Gel tray 15 x 17 cm has 6 spaces for combs insertion e Gel tray 15 x 12 cm has 4 spaces for combs insertion e Gel tray 15 x 7 cm has 2 spaces for combs insertion Conventional compatible with ONE FREE GEL TRAY WHEN ORDERING 2 ADDITIONAL COMBS multichannel pipettes and preparative combs TECHNICAL SPECIFICATIONS e 4 adjustable feet and bubble level Buffer Volume 1 200 ml e Red strips for wells vizualisation Unit dimensions e Ports for buffer circulation w x Lx h 30 x 20 x 8 cm ORDERING INFORMATION 370000 MIDIGEL XL Includes Removable U V transparent gel tray 15 x 17 cm w x l 2 removable casting gates 2 0 mm thickness 27 teeth comb sample volume 32 ul safety lid set of power leads buffer circulation accessories user s manual 3700PB PACK B Includes MIDIGEL XL Fully equipped as above Cat No 370000 Power supply PS 304 371000 MIDIGEL XL Fully equipped with 6 combs Cat No 371601 for running 96 samples on a 15 x 17 cm gel Includes removable U V transparent gel tray 15 x 17 cm w x l equipped by 2 removable casting gates U V transparent gel tray 15 x 12 cm w x equipped by 2 removable casting ga
115. s Symptom torted band pattern dyes run normally ELECTROPHORESIS TROUBLESHOOTING B Sample Preparation Degradation of samples leads to smeared or doubled bands Overloading will cause bands to smear and will alter band positions 1 2 3 4 Nudease degradation Exonucleases will cause bands to smear down endonucleases will generate new bands Keep samples cold include EDTA where practical Denaturation Insufficient heating may allow retention of residual secondary structure which causes bands to migrate at spurious molecular weights and may cause doublets Overloading Loading of more than 10 ug lane of DNA will cause upward smearing of the bands Urea in wells The urea in the gel will diffuse into the wells and disrupt Sample loading Flush wells with running buffer just prior to loading Diagnosis Gel poorly polymerized 2 gel overloaded B 3 nuclease in samples B L salt in samples 0 1 or wells not flushed B 4 d storted band pattern dye run speed altered Buffer mismatch D 1 3 4 glycerol in samples D 5 run parameters not optimal C l or fixed charges on gel 4 a nes run faster than outer lanes Gel heating during run check run parameters C L buffer 0 3 4 and heat exchange system on gel apparatus C 3 Poor gel polymerization A 2 or upper edge of gel distorted A 3 Samples not fully denatured 2 nuclease degradatio
116. s s Panneau lexan avec switches tactiles pour r glage rapide et pr cis Trois modes op ratoires R gulation de voltage courant et puissance Trois LEDs indiquent le type de param tre r gul Il est possible de programmer une r gulation avec limitation pr cise des autres param tres Un param tre peut tre programm pour r guler durant toute la migration Trois switches permettent de commander les fonctions suivantes e START avec LED vert e STOP avec LED rouge PRESET permettant l affichage des pr r glages pendant la migration Trois afficheurs pour lecture simultan e et individuelle des parametres pr r gl s et d bit s Trois sorties prot g es pour d bit sous haute tension Recommand pour le s quencage des acides nucl iques mais utilisable pour les lectrophoreses sur gels d agarose et gels polyacrylamide Coupure automatique avec alarme sonore en cas de fuite lectrique la terre court circuit circuit ouvert cuve d branch e ou non branch e surcharge soudaine Un message s affiche simultan ment indiquant le type de faute l origine de la coupure du d bit En cas de situation de faute un voyant lumineux Fault clignote sans interruption associ une alarme sonore temporis e sur 10 secondes Apres coupure et retour du courant secteur une alarme sonore se d clenche pendant 10 secondes puis le g n rateur relance son d bit automatiquement en respect des va
117. t Hr steps 1 9999 minutes 1 minute steps 10 Volt 1 mA 1 watt 0 1000 Volt 10 Volt 0 600 mA 1 mA 0 300 watts 1 watt 90 ace 0 99999 Volt Hr 1 Volt Hr steps 0 9999 minutes 1 mn steps 10 Volt 15 pA 0 3 W 10 Volt 0 mA 0 W Fault detection Dimensions w x d x h stop audible alarm and displayed messages 27 x 34 x 11 cm stop audible alarm and displayed messages 27 x 34x 11 cm MES SUPPLIES POWERFUL EASY TO USE ROBUST PS 3003 Cat No 178000 3000 Volts e 300 mA 300 Watts Constant Voltage e Constant Current e Constant Power FEATURES Last settings are automatically restored at power up Tactile switches for precise and easy input of parameters Three operating modes Constant voltage constant current constant power Three red LEDs indicate the constant parameter It is possible to perform electrophoresis with constant mode whilst limiting the others to selected values e g constant power whilst limiting current and voltage Automatic crossover from one mode to another when preset output limits are reached Three push buttons permit selection of the following functions e START with green LED e STOP with red LED e PRESET for reading the preset values during electrophoresis Automatic RESTART in case of power failure during a cycle When the power returns an audible safety alarm rings for 10 seconds
118. tes 6 x 1 0 mm thickness 16 teeth comb compatible with multichannel pipettes sample volume 24 ul safety lid set of power leads buffer circulation accessories user s manual 371696 PACK 96S Includes MIDIGEL XL Fully equipped as above Cat No 371000 Power supply PS 304 XL Thickness Teeth Sample volume Well width 371603 Comb 3 0 mm 16 72 ul 6 0 mm 372703 Comb 3 0 mm 27 48 ul 4 0 mm 37P112 Prep Comb 2 0 mm 1 sample 1 marker 280 48 ul 138 5 mm 340500 Separate gel Casting base with adjustable feet and bubble for a 5 mm thickness gel compatible with multichannel pipettes A TANKS FOR NUCLEIC ACIDS NUMBER OF COMBS AND GEL DIMENSIONS 15 cm 15 cm 15 cm 15 cm 20 cm 12 cm 7 cm Gel tray Cat No 371520 Gel tray Cat No 371517 Gel tray Cat No 371512 Gel tray Cat No 371507 8 comb slots 6 comb slots 4 comb slots 2 comb slots SUGGESTED POWER SUPPLIES Page 6 APELEX PS 304 XL O pes w PS 304 XL Cat No 160600 SUGGESTED BLOTTING TANKS Pages 42 43 Mini transblotter Cat No 440000 Slim blotter Cat No 504017 PACK 965 Cat No 371696 INCLUDES MIDIGEL XL for 96 samples Cat No 371000 PS 304 XL PS 304 XL mH oo oe NE START um 0 0 0 0 STOP L Page awe Cup NUCLEIC ACIDS MAXIGEL ECO 2 APPLICATION DNA RNA ana
119. trophor se pr parative Transfert Semi Sec Western Southern et Northern Blotting Conseill Possible 09 d PROGRAMMABLES PS 9009 TX Ref 119030 3000 Volts e 300 mA 300 Watts DANGER HIGH VOLTAGE ee PS 1006 P Ref 119050 1000 Volts 600 mA 300 Watts CARACTERISTIQUES Les derniers param tres programm s sont automatiquement m moris s M moire capable de stocker e 16 programmes avec 1 temps unique de migration e 8 programmes s quenc s poss dant chacun 10 modules se succ dant automatiquement en fonction de leurs programmations individuelles 1 programme sp cifique pour faibles courants inf rieurs 15 pA IEF Affichage LCD avec 2 lignes de 20 caract res Clavier 20 switches tactiles sur panneau membrane Panneau membrane avec clavier tactile pour r glage pr cis des param tres Nettoyage facile Quatre modes op ratoires Voltage constant courant constant puissance constante ou consigne de limitation de temp rature TEMPERATURE CONTROLLED gt 088006600000 DANGER HIGH VOLTAGE PROGRAMMABLE ELECTROPHORESIS POWER SUPPLY 904688 ea50 0890668800 DZ Il est possible de programmer une r gulation avec limitation pr cise des autres param tres Un param tre peut tre programm pour r guler durant toute la migration e R gulation de Voltage courant et puissanc
120. wer leads user s manual Thiknes Samplevolume Wellwidth 300052 Comb 2 0 mm 5 48 ul 6 0 mm 301115 Prep Comb 1 5 mm 1 sample 1 marker 240 ul 15 ul 40 0 2 5 mm 340500 Separate gel Casting base with adjustable feet and bubble for 5 mm thickness gel Cat No 160600 Sax e Page APELEX PS 305 xL Bo EI O 5 Hee 0000 O lt w lt MiniGel Transblotter Cat No 430000 Page 42 Slim blotter Cat No 504002 Page 43 TANKS FOR NUCLEIC ACIDS MINIGEL 2 Gel dimension 10 x 8 cm w x 2 comb Slots APPLICATION Designed for very fast separations and small scale procedures of nucleic acids restriction fragments in agarose gel e Compact size Whole unit can be placed on U V table TECHNICAL SPECIFICATIONS gt Buffer Volume 250 ml Maximum number of samples 36 Unit dimensions wx Lx h 19 16 000000 ONE FREE GEL TRAY WHEN ORDERING 2 ADDITIONAL COMBS ORDERING INFORMATION 311000 MINIGEL 2 Includes Removable U V transparent gel tray 10 x 8 cm w x 1 2 removable casting gates 1 0 mm thickness 16 teeth comb sample volume12 ul safety lid set of power leads user s manual 3110PA PACK A Includes MINIGEL 2 Fully equipped as above Cat No 311000 Power supply PS 304 XL Thickness Teeth Sample volume Well width 320101 Comb 1 0 mm 10 24 ul 6 0 mm 320102 Comb 2 0 mm 48 ul 6 0 mm 320161 Comb 1 0 mm 16 12 ul 3 0 mm 320162 Co

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