Nichipet EX II
Contents
1. Insert the filter remover into the filter and pull in the direction of the arrow Insert a new filter into the nozzle A Do not touch filters which may be contaminated by harmful substances CAUTION Take extreme care for sharpness of Nozzle filter remover when filters for 5000 ul or 10000ul S are taken off CAUTION Nozzle filter remover Dinngna ut CO U ee Nozzle filter 210 0D Tu EDD UO Out Nozzle filter remover Nozzle filter Fig K 217 Autoclaving This pipette is autoclavable When autoclaving carry it out at 121 C for 20 mimutes following the procedure mentioned below For the 1000 ul 5000 ul and 10000 ul types remove the filter referring to the Filter replacement procedure mentioned on P16 Release the lock lever from the locked position and set the counter graduation to the allowable limit of the liquid volume After autoclaving is complete dry the pipette completely Note When autoclaving don t pile pipettes on others in the autoclave or lean pipettes with a nozzle top facing down so that self load is applied on the nozzle This pipette is made of an autoclave compatible material but because of high temperature in the sterilizer there is a risk that parts subject to load will be deformed Drying the pipette Dry the pipette immediately after autoclaving is complete It is necessary to dry the pipette with a constant temper2. The shape of the O ring retainer differs depending on the type of the pipette For the 20ul type remove the fluoroplastic PTFE spacer located inside 5000ul 10000ul Fig G4 G5 Remove the seal spring the O ring retainer the O ring and the seal ring from the nozzle cylinder Note Shapes of the O ring retainer are different by size of pi e Note Pay attention to small parts for not loosing them during disassembling 9 2 Reassembling Reassemble nozzle cylinder 2 10 20ul Fig G1 100 200ul Fig G2 1000ul Fig G3 Firstly set 1st spring on plunger next set O ring retainer seal ring and O ring in this order Then insert assembled plunger into nozzle cylinder and screw it into the body 5000ul Fig G4 10000ul Fig G5 Set volume setting below 3096 of maximum volume Assemble the seal ring the O ring and the O ring retainer together and place it on the seal spring Fig H 1 Place O ring side on the ground and insert the plunger into the seal spring until the O ring is fully inserted Take extreme care not to bend or twist the seal ring when inserted to the plunger Fig H 2 Put the nozzle cylinder over the plunger with the seal spring the seal ring the O ring retainer and O ring and screw securely to the lower body Fig H 3 Note For 1000ul 5000ul and 1000ul make sure the seal ring and the O ring are securely assembled Strained or twisted seal ring or O ring may cause leakage
3. and reached to a bottom of many tubes for dispensing aspiration 110mm in depth 2ul 1000ul e Tip can be removed without touching by using the tip ejector e The Easy Calibration function provides smooth and easy adjustment with an attached adjustment tool Standard accessories e Three tips one tip for NPX2 5000 10000 e Three nozzle filters NPX2 1000 5000 10000 e A nozzle filter remover NPX2 1000 5000 10000 e An adjustment tool e User s Manual When unpacking package check to make sure that the above mentioned items are included Precautions on safety e For using your Nichipet EX II properly and safely carefully read Precautions on safety in this paragraph and CAUTION on the next page before starting work with it e Contents of CAUTION are matters that require user s attention not only for using Nichipet EX II properly but for preventing user from accidents and physical damage eAfter reading this manual please keep it in a convenient place for referring to at any time Please read following prior to use for your safety and correct usage N CAUTION Be sure to observe the following instructions for using Nichipet EX II properly and safely If user misuses Nichipet EX II or disregards the following instructions it may result in injury to the user or and other persons or physical damage to pipette or and other equipment D JO BB CUN HG o 10 11 12 13 14 15 16 17 18
4. of recipient vessel just above liquid surface 10 degrees to 45 degrees in angle 2 Press down the push button slowly and smoothly to the first stop b Wait for a few seconds then press down the push button to the second stop to expel the last drop of the liquid from the tip Fig D 3 Release the push button slowly 4 Press the ejector button to remove the tip and dispose of it Fig D When using any toxic or harmful liquid to human do not touch any used tips CAUTION ow Lt TEN Fig D 4 Recommendation for accurate pipetting In addition to the above mentioned operations of pipetting the following procedure maximizes performance of pipette 1 Make sure that tip is firmly attached to nozzle end 2 Before pipetting pre rinse the tip by filling and expelling the tip for three to five times It gives good precision of pipetting especially with a viscosity or a vaporous liquid So M a Lj Ejector button WU Fig E 3 Especially with volumes under 50ul the operation should be done very slowly and smoothly and the air humidity should be as high as possible to reduce the effect of evaporation loss 4 Sampling high density liquids an
5. 00020 Firstspring20u fw 20ul 00 NX2 0200100 First spring 100ul 100ul 00 NX2 0200200 First 200ul OOCOCOCOHLIOCCOCOOCOOCOCCOOOUD 00 NX2 0201000 First 1000ul 00000000000000 00 NX2 0400002 Nozz 2ul 00 NX2 0400010 Nozzle cylinder set 10ul 10ul NX2 9 O ring 00 NX2 0400020 Nozzle cylinder set 20ul retainer nozzle cylinder 20ul 00 NX2 0400100 Nozzle cylinder 100ul 100ul 00 NX2 0400200 Nozzle cylinder 200ul 2 200ul 00 NX2 0401000 Mazzle culincier OO 1000ul 000000000 24 L0000000000000 00 NX2 0405000 5000ul 000 DBOHDDUODO BU 00 O0 00 NC 0500020 00 NX2 0410000 10000ul 00 NX2 2205000 Seal spring 5000ul 5000ul 00 NX2 2210000 Seal spring 10000ul m 10000ul 00 NX2 0500100 O ring seal ring set O ring seal ring O ring 100ul 100ul retainer RR O ring seal ring set O ring seal ring O ring 00 NX2 0500200 200ul retainer 200ul 00 NX2 0501000 O ring seal ring set O ring seal ring O ring 1000ul 1000ul retainer 24 O ring seal ring set O ring seal ring O ring 00 NX2 0505000 5000ul retainer 5000ul 00 NX2 0510000 Padog Seal ring set TAU E 10000ul 00 NX2 0900010 EjectorpipeA ff 2 10ul 00 NX2 0900200 Ejector pipe B f 20 100 200ul 00 NX2 0901000 Ejector pipeC 1000ul 00 NX2 0905000 Ejector pipe D e
6. 5000ul 00 NX2 0910000 Ejector pipe E 100000ul 00 NX2 0801000 Ejector setting screw set EE s Ge 00 NX2 0815000 Ejector setting screw 5000ul 10000ul 00 NX2 0601000 Nozzle filter 10pcs 1000ul 00 NX2 0615000 Nozzle filter 100pcs 5000ul 10000ul 00 NX2 1100000 Nozzle filter remover 1000ul 00 NX2 1500000 Nozzle filter remover 5000 10000ul 00 NX2 1600000 Volume adjustment tool en All OCCOCCO0COO0 25 26 For repair service or information you may contact your local distributor Manufacturer ONICHIRYO CO LTD Tokyo office 2 Kandamikura cho Chiyoda ku Tokyo 101 0038 Japan TEL 81 3 6661 2602 English FAX 81 3 6661 2604 URL http www nichiryo co jp E mail i sales nichiryo co jp 2011 Ver 1
7. Autoclavable amp UV resistant Nichipet EX II Digital micro pipette for liquid handling User s Manual In Vitro Medical Diagnostic Devices 98 79 EC Annex III self declared ISO 8655 STANDARD CERTI FI ED I S009001 e Thank you very much for purchasing Nichipet EX II e Please read this manual carefully before using Always Pursuing Originality BE ORIGINATIVE oe NICHIRYO Autoclavable amp UV resistant Nichipet EX II Digital micro pipette for liquid handling Features e Nichipet EX II is fully autoclavable at the condition of 121 C for 20 minutes e Nichipet EX II is made of UV resistant material which can be used in clean bench If UV is applied to Nichipet EX II for a considerably long time it may become discolored but nothing affects its performance e New round shape improves friendly handling capability and mitigates operator s fatigue from long time use e Sample volume can easily be set by simply rotating the push button e Setting of sample volume can easily be locked with one touch one touch lock mechanism e A wide range of sampling volume can be covered by eight models from 0 1ul to 10 000ul e Patented body construction avoids permeating hand temperature through the body that prevents inaccuracy of volume measurement e Since PTFE Fluoroplastic is used in an airtight chamber this combination keeps airtight and precise reproducibility for long hours e A thin nozzle corn can be easily inserted
8. Do not use pipette for any purpose except pipetting dispensing liquid Do not modify pipette because modification may cause an accident Do not use pipette for pipetting any liquid to be injected into human body Do not expel dispensed liquid towards anybody Do not eject tip towards anybody Do not eject tip with liquid inside Carefully handle pipette and tip because tip is sharply pointed If pipette is contaminated with liquid harmful to human immediately take appropriate disposal to clean it safely before continuing to work Do not use pipette for stirring liquid and so on otherwise not only tip may be loose and fall off but the pipette may be soiled with scattered liquid Do not touch filters which may be contaminated by harmful substances Take extreme care for sharpness of Nozzle filter remover when filters for 5000 ul or 10000ul types are taken off Do not touch pipette directly right after drying because it will have got very hot during drying Touching hot pipette directly may cause injury Components of water used for autoclave may cause pipette malfunction or performance If pipette can not be fixed after examining and conducting in accordance with designated procedure immediately stop using the pipette and ask us or our agent to repair it For organic solvents Nichipet EX Plus II NPLOIT will be recommended to use Although this pipette has good chemical resistance in general it may be damaged by som
9. Note When screwing into the body make sure that the nozzle cylinder and the body are in vertical direction and screw the nozzle cylinder into the body very gently If not it may cause leakage Note When reassembling be careful not to put the seal ring and O ring in the wrong order If the order is wrong it may cause not only leakage but also inaccuracy failure in extracting liquid etc Fit the ejector pipe to the body 2 10 20ul Fig G1 100 200ul Fig G2 1000ul Fig G3 While pressing down the ejector button with your finger so that the metal stay sticks out from just under the grip insert the ejector pipe into the body and fix it with the ejector setting screw When ejection needs extra strength snugly adjust position of the ejector pipe Fig J Note After reassembling repeat trial operation several times and inspect that there is nothing wrong with the pipette 10 2 O ring retainer Body Seal ring G Nozzle cylinder O ring set CD Nozzle cylinder Enlargement of seal ring To nozzle cylinder Ejector setting screw Q 1st spring Ejector pipe Fig G1 11 COOO CO0 0000 000 Des O ring retainer Nozzle NS amp Seal ring cylinder set Body o ring Nozzle cylinder Plunger set Enlargement of seal ring To nozzle cylinder Q 1st spr
10. ature air drier at 60 C for 60 minutes or longer Pull out the ejector pipe referring to Disassembling on page 7 Q Turn the nozzle cylinder counterclockwise by two and a half turns to loosen it Put the pipette in a constant temperatures air drier for drying After the pipette is dry wait until it returns room temperature and then fasten the nozzle cylinder and reassemble the ejector pipe into the body Note If the pi is reassembled when it is still warm it may cause breakdown or deterioration of the pipette such as breakage of the screw threads etc Be sure to reassemble the pipette after it has completely cooled down CAUTION v x e Components of water used for autoclave may cause pipette malfunction or performance 18 Specifications Nichipet EX II is a high quality pipette The technical figures given in the Table 1 Nichipet EX II Maximum Permissible Errors were obtained using genuine Nichiryo BMT Tips Nichiryo declares that pipettes comply with the requirement of the ISO 8655 Standard by type testing The adjustment is carried out under strictly defined and monitored conditions ISO 8655 6 e The basis of adjustment EX e Reference temperature 20 C 25 C e Relative humidity more than 50 e Barometric pressure 101KPa e Use of distilled water distilled water e Ten measurements for each test volume at three points nominal volume 50 of nominal volume and the minimum or 1096 of no
11. d viscous solutions When aspirating once the liquid has entered the tip wait 2 to 3 seconds before removing the tip from the surface of the liquid When dispensing wait 2 to 3 seconds at the first stop position before pushing to the second stop position Disassembling Reassembling the airtight chamber If such symptoms as mentioned in Troubleshooting page 21 occur disassemble and inspect pipette according to the following procedures 1 Disassembling Remove an ejector setting screw 2ul 200ul Fig F 1 1 Turn the ejector setting screw to the direction of the arrow to remove it and then pull out ejector pipe to the direction of arrow 5000ul 10000ul Fig F 2 1 Turn the ejector setting screw to counterclockwise to remove it and then pull out the ejector pipe to the direction of arrow 00 00 Ejector setting screw 00 00 45 Ejector pipe 00 00 0 TT mcns Bil Nozzle cylinder CS Lower part Fig F Nozzle cylinder Turn the nozzle cylinder to counterclockwise to remove it as it is screwed into the main body Note When removing the nozzle cylinder take care with the internal parts as some of them occasionally spring out of the body for s of 2ul to 1000ul volume Remove internal parts one after another 2ul 1000ul Fig G1 G2 G3 Remove the plunger 1st spring O ring retainer O ring and seal ring from the nozzle cylinder
12. d with liquid 1000ul or larger Pipette fails to has a filter aspirate liquid Seal ring and O ring are Reset the seal ring and E O ring set according to the manual in E Disassembling reassembl Seal ring or and O ring set E are worn D f Replace an seal ring and NE D Tighten up the nozzle inq linder securelv Nozzle cylinder is worn ev x stepped wear can be D Replace an nozzle cylinder checked by eye Replace an seal ring and Seal ring and O ring set is E O ring set worn because the plunger ds caida d Replace an seal ring and E The tip is loosely attached D G R E If the push button does not work well after dispensing or it is sticking to the body disassemble the pipette and wash clean every Extracted liquid leaks from tip The tip is loosely Push button attached moves stiffly part or wipe down every part with soft cloth If there are some parts getting rusty or corroded inside the body replace the parts with new ones If the pipette can not be fixed after examining and conducting the above A mentioned procedure immediately stop using the pipette and ask us or our CAUTION agent to repair it Before bring the pipette for repair be sure to check whether it has been contaminated with microbes or harmful substance 22 Consumables eTip Autoclavable Volume range Tip length Code ul Color Applicable pipett
13. e mm Q ty BMT UT 0 1 10 Clear NPX2 2 31 0 1000 BMT SS 0 5 10 Clear NPX2 10 45 5 1000 BMT SG 2 200 Clear NPX2 20 100 200 53 0 1000 BMT L 100 1000 Clear NPX2 1000 77 0 1000 BMT X 1000 5000 Clear NPX2 5000 132 0 200 BMT Z 1000 10000 Clear NPX2 10000 158 0 200 eRacked tip Autoclavable Code oie Ga Color Te Q ty BMT UTR 0 1 10 Clear NPX2 2 960 96pcs x 10 cases BMT SSR 0 5 10 Clear NPX2 10 960 96pcs x 10 cases BMT SGR 2 200 Clear NPX2 20 100 200 960 96pcs x 10 cases BMT LR 100 1000 Clear NPX2 1000 1000 100pcs x 10 cases BMT XR 1000 5000 Clear NPX2 5000 100 50pcs x 2 cases 23 Spare Part list Code Description Content Type in volume 00 NX2 0100002 Plunger set 2ul Plunger head plunger 2ul 00 NX2 0100010 Plunger set 10ul Plunger head plunger 10ul 00 NX2 0100020 Jer head plunger 20ul 00 NX2 0100100 ger head plunger 100ul 00 NX2 0100200 ger head plunger 200ul 00 NX2 0101000 ger head plunger 1000ul Plunger Joint TBA Plunger set 5000ul Vy 3l ing joint shaft 5000ul jomt plate Plunger joint TBA Plunger set 10000ul block w O ring joint shaft 10000ul joint plate 00 NX2 0200002 First spring 2ul 2ul 00 NX2 0200010 First spring 10ul fw 10ul 00 NX2 02
14. e kinds of chemicals Please contact to our company when using strong chemicals Pipette can be used between 4 C and 40 C but the specifications may vary Depending on frequency of use pipette should be cleaned in a soap solution and airtight chamber should be maintained according to this manual D Note Users are required to strictly observe the followings in order for the pipette to keep its excellent precision reproducibility and original performance for a long time 1 Do not expose pipette directly to the sun when working with it or for 2 hours before starting work otherwise the pipette may lose accuracy Avoid working with pipettes in a humid and hot place 2 Just before starting work with pipette avoid touching tip and nozzle cylinder as far as conditions are allowed If nozzle cylinder is warmed by your hand accuracy may vary 3 For pipetting follow the forward method the way explained in this manual If it is performed in a different way it may result in inaccurate pipetting 4 Operate push button very gently If it is quickly released it may result not only inaccurate pipetting but also deteriorated the pipette because sample liquid may be permeated into the main body To prevent the pipette from malfunction inaccuracy and contamination a filter is attached to models NPX2 1000 5000 and 10000 A filter is supplied at time of purchase 5 Do not reuse tip that has been used once and carefully dispose used tip I
15. f tip is used repeatedly it may cause inaccurate and impure pipetting and cross contamination among samples For example if previous sample liquid is left inside tip it is mixed with new sample liquid and the new sample is contaminated by the previous one Therefore pipetting of the next sample results wrong This phenomenon is called mutual contamination of samples 6 Do not hold pipette horizontally or upside down when there is liquid inside tip otherwise the liquid gets into the main body and the pipette may be contaminated 7 When autoclaving do not pile pipettes on others in the autoclave or lean pipettes with a nozzle top facing down so that self load is applied on the nozzle This pipette is made of an autoclave compatible material but because of high temperature in the sterilizer there is a risk that parts subject to load will be deformed 8 After autoclaving and drying pipette leave it until it gets completely cool before using again If the pipette is used when warm the accuracy may not come up to the standard level 9 After autoclaving and drying pipette assemble the pipette after it is completely cooled if it is assembled when it is still hot it may cause deterioration in the pipette such as breakage of the screw threads 10 When rotating push button do not exceed the specified sample volume limit otherwise pipette may be damaged or deteriorated 11 Do not perform pipetting with less liquid than set volume If
16. ing Ejector setting screw Ejector pipe Fig G2 12 HOUU oooU Nozzle cylinder Plunger set Nozzle Filter 1st spring Ejector setting O ring retainer Sere O ring Gei Seal ring D seal ring set O ring Ejector pipe Enlargement of seal ring_ To nozzle cylinder Fig G3 13 DUU 00DD Body Nozzle cylinder Lower Plunger set Body Nozzle Filter Plunger GSealspring Ejector setting screw O ring GEjector pipe retainer O ring seal ring set Seal ring Enlargement of seal ring Ha O ring C To nozzle cylinder Fig G4 14 0000 00000 Body Nozzle cylinder Plunger set G Nozzle Filter f Ejector Seal spring setting screw O ring ER Ejector pipe retainer O ring seal ring set Seal ring Ci Enlargement of seal ring_ D IC OI To nozzle cylinder Fig G5 15 Filter replacement procedure 1000ul Fig KD Insert the tips of filter remover into two notches on the sides of a filter and pull out the filter in the direction of the arrow Set the projection of a new filter in the internal groove of the nozzle and then press the filter into the nozzle 5000ul 10000ul Fig K
17. minal volume Table 1 Nichipet EX II Maximum Permissible Errors Accurac Precision iss m D ange enne Gees random error error AC CV 0 2 12 0 lt 6 0 NPX2 2 0 1 2 ul 1 15 0 25 2 3 0 lt 1 0 1 4 0 lt 3 0 NPX2 10 0 5 10 ul 5 1 0 lt 1 0 10 1 0 lt 0 5 2 15 0 lt 3 0 NPX2 20 2 20 ul 10 1 0 lt 1 0 20 1 0 lt 0 4 10 2 0 lt 1 0 NPX2 100 10 100 ul 50 1 0 lt 0 3 100 0 8 lt 0 3 20 1 0 lt 0 5 NPX2 200 20 200 ul 100 0 8 lt 0 3 200 0 8 lt 0 2 100 1 0 lt 0 5 NPX2 1000 100 1000 ul 500 0 8 lt 0 3 1000 0 7 lt 0 2 1000 1 0 lt 0 3 NPX2 5000 1000 5000 ul 2500 0 8 lt 0 3 5000 0 6 lt 0 2 1000 2 0 lt 0 4 NPX2 10000 1000 10000 ul 5000 0 8 lt 0 3 10000 0 4 lt 0 2 Obtained values for accuracy and precision may vary by familiarity of user especially less than 0 2ul with NPX2 2 The data given in the table conform to the ISO 8655 6 standard 19 Volume setting procedure 1 Loosen the lock lever 2 Depress the tip ejector button fully Fig L 3 Loosen the lock lever by turning it to counterclockwise and stop when the oval opening under the lever faces over the tip ejector button Fig L Lock lever m Hex head screw Ejector button 4 Rotate the push button until one of two hex head screws comes to the top of oval opening Fig L 5 Lo
18. ol fo o o 9 fol fo 0 fo maul MW oul A SAU ye sr Point Mark 20L 4L 20L JUL 20011 1QuL 5m Wm 0000 001 00 01 02 1 10 Minimum scale ul Fig B 2 Aspiration liquid 1 Attach a new tip to the nozzle end We recommend that you attach it from rack Note It would be recommended that tips are directly picked up from rack And do not twist pipette when fixing tip 2 Press down push button to first stop position b Fig C Don t aspirate the liquid with the push button pressed at the second stop c 3 Hold the pipette vertically and immerse the tip 2mm to 3mm below the surface of the liquid Fig D 4 Release the push button slowly and smoothly to aspirate the set volume of the liquid Fig D 5 Wait 1 to 2 seconds then withdraw the pipette vertically and carefully from the surface of the liquid Fig D 6 Wipe any droplets away from the outside of the tip using a medical wipe and avoid touching the tip s orifice Note Do not aspirate when push button position is at c Note The push button has to be pushed and released slowly Otherwise it ma cause wrong accuracy a b c TES e L4 Ejector button E Fig C eeu 3 Dispensing liquid 1 Gently place the end of tip against inside wall
19. osen both hex head screws with a hex head wrench 1 5mm by turning them to counterclockwise one by one Fig L A Increase 6 Keeping the hex head wrench inserted Cu B Decrease into one hex head screw turn the push button to calibrate the pipette Fig M 7 The pipetting volume can be adjusted by rotating the push button to clockwise to increase and counterclockwise to decrease Please refer to the volume correction table Hex head wrench Volume correction table Range Correction Value at Each Graduation The Minimum Graduation NPX2 2 0 0013 ul 0 002 ul NPX2 10 0 0079 ul 0 01 ul NPX2 20 0 0128 ul 0 02 ul NPX2 100 0 0797 ul 0 1 ul NPX2 200 0 1269 ul 0 2 ul NPX2 1000 0 7952 ul 1 0 ul NPX2 5000 7 9960 ul 10 0 ul NPX2 10000 8 0359 ul 10 0 ul 8 Tighten the both hex head screws after adjusting the push button and measure the accuracy of the pipette 9 Repeat the above procedures until the pipette is calibrated within the specified accuracy An accuracy test should be made at the specified minimum and maximum volume of each pipette 20 Volume measurement lt Procedure gt In order to avoid influence from temperature prepare pipettes for inspection distilled water balances and tips 2 to 3 hours before using at where measurement is conducted Measurement room should be controlled temperature between 20 25 C and measurement
20. should be held at where there is no direct wind from Air conditioner Pick up a proper tip from tip rack and aspirate sample water Dispense the sample for measuring with a balance Read volume measurement by the balance and compute accurate volume by following formula Vi Volume Va In X Z mi Measured volume l l r Z Z correction factor Add all 10 time Vi volumes and divide the sum by 10 to compute a mean volume 1 n V x V 10 n l Calculate the systematic error of the pipette where V is the selected test volume DE e 100 A V S Calculate the coefficient of variation CV by formula for standard deviation Table 3 Z correction factor for distilled water Air Pressure kPa Temperature tC 95 100 101 3 105 20 0 1 0028 1 0028 1 0029 1 0029 20 5 1 0029 1 0029 1 0030 1 0030 21 0 1 0030 1 0031 1 0031 1 0031 21 5 1 0031 1 0032 1 0032 1 0032 22 0 1 0032 1 0033 1 0033 1 0033 22 5 1 0033 1 0034 1 0034 1 0034 23 0 1 0034 1 0035 1 0035 1 0036 23 5 1 0036 1 0036 1 0036 1 0037 24 0 1 0037 1 0037 1 0038 1 0038 24 5 1 0038 1 0039 1 0039 1 0039 25 0 1 0039 1 0040 1 0040 1 0040 21 Troubleshooting Tip cannot be E Tighten up the nozzle ejected Nozzle cylinder loosens D cylinder securely Replace the filter with a new one supplied as a standard accessory or purchase a filter set for replacement Filter is soake
21. the quantity of liquid is less than the set volume it may cause the liquid to scatter into the main body and the pipette may deteriorate in quality Contents Operating procedure hri terrenet te 5 Disassembling Reassembling the airtight chamber vv 9 Autoclaving Drying the pipette mmm 18 Specifications imme 19 Volume setting procedure meme 20 Troubleshooting UV 22 pe ea ca rer qe E ANIM aM DIS 23 Operating procedure 1 Volume setting 1 Turn the lock lever to unlocking direction to loosen it Fig A 2 Turn the push button to set the digital counter to a desired liquid volume To increase volume setting turn the push button until passing designated volume setting by half of the scale and then set the designated volume To decrease volume setting simply turn to designated volume When setting the liquid volume set the counter s graduation at point mark red appearing in the lower part of counter window Fig B 3 After setting the liquid volume turn the lock handle to locking direction to lock it Fig A Note Don t exceed the specified liquid volume limit otherwise pi e may be damaged or deteriorated in the quality C Decrease OB Push button P Increase Lock lever Ejector button Fig A Volume indicator PX2 2 NPX2 1 PX2 20 WU NPX2 200 RO ID NPX2 5000 NPX2 10000 1 1 1 1 2 0 2 0 2 0 5 0 ch fo Jo lo o l
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