manual MedCalc Softw are
Contents
1. 650325 023456 Referrer 1 SPOCK New Patient name Adam Dr Spock Abbey Road 2 Date of birth 01 12 1970 London Partner name Eve Referrer 2 Date of birth 30 04 1973 Subgroup Ejaculation date Reference time Motes OR Abstinence days Cancel Required input Patient record number a record number that identifies the patient e g hospital record number Patient name the patient s name enter his last name first Date of birth the patient s date of birth Partner name the name of the partner Date of birth the partner s date of birth e Subgroup select the code for the subgroup this sample will be classified into e g PATIENT or DONOR for patients or sperm donors e Ejaculation date and time data and time of ejaculation e Abstinence days the number of days since the patient previous ejaculation e Referrer 1 and 2 select the code of the referring doctor s If the referrer is not in the list click the New button The date of the selected referrer are displayed below the code Click Edit if you need to make corrections e Reference enter a reference number Buttons e Print print a complete report e OK saves the data in the database and closes the dialog box e Cancel closes the dialog box without saving the data in the database Tips When you click Print the report will immediately be printed to the default printer If you want to select a printer first press Ctrl P or func2. Change Login Command File Change Login Description Allows to login as another user without having to exit the program Login Description Allows to access the AutoSperm software and database Required input AutoSperm 5 Login e Login your login name e Password your password Remember that your password is case sensitive To change your password After you have successfully logged in with your current password select Users in the Tools menu referrers select Referrers in the Tools menu Next select your login name in the list and click Edit First login after installation of the program After you have installed the AutoSperm software you use ADMIN for login name as well as for password This will allow you to login as the AutoSperm administrator Next e change your password see above e create user accounts for one or more technicians Even if only one person will use the software you must create a new user account and use this account to access to program for daily work As an administrator you cannot perform semen analyses in the AutoSperm system Page Setup Command File Page Setup Description Allows to set the margins of the printed report Required input Enter the size in millimeters of the top left right and bottom margins for the printed report Exit Command Description Stops the program Shortcut Alt F4 New Sample Command Sample iNew Sample De
3. ooocccoccccoccconcncncncnncnnnnnnoncnnnnnnoncncnncnnnnnnonrnnnnnnoncnnos 6 Autosperm software installation ni n A Ai A 7 RUANO the program tor the first UM ii haven ee ee ee ee N 10 Setup TINA SLC aaien e e O nse R ORE E RT RT erR a E RER 11 GOUNTING ProceduU le uici sicianni iniciado 12 MENU OVONVICW A A AAA RA 14 Changs LLO ia A A A A A A A A aeons 14 E O A 14 FAC A O see 15 EX etnia ted ole cod Le lees oh tele Llob a Ele a oh e lee Gh shale LdOh da Eb sled 15 NeW S amplia 15 Sampe dialogens A i ert net mene a erent eer tee rerio a een enter eee erect ener ere ee 16 A A A 16 A A O a 17 Additional MAS ES A A cranial et 19 VAMO A A a 20 A A ieee ms eerie te etree renner ine OAE Arne oe me ere ene 20 La SOU Mell PA on A Aeon ue A oe eens eisai asaune ates eee 22 Samples Requiring Validation coooccccoooncccononononccoconconononononcononcnononconannnnnoncnnonnnononnnnancnenannanos 23 MOMAY AW ClaGCS lle da col bale Col io 24 EXPO Dalma a iond 25 USCIS iio ti ir A a A io 25 USO amet read edit olas loca iat ena ee ae 26 A el a a ue E aa leh rales ee eae yee ale hi 26 Relee di O NA A daar OR 26 calculate VO UNE ae Doo cOS 27 LESA o ds eee 27 A O O taaetacecsteceacteeetea 28 OPUONS A A A ae ee ee ne ee 29 PAVANY S S SO MIMI nd a ia peas do bani ian a cidos 29 Tal anciano aro aca 30 A A A E A enc aee 30 Additonal anal SES utili 31 VOXUEMACIOS osne oon sdiUond ua 31 WISCEIANGOUS ODUONS aia A aa 31 E o Stee fits ete
4. etc Address the address to send printed reports to Phone phone number 26 e Mobile mobile phone number e Fax fax number e E mail his her email address e Active mark the user as active If the referrer is not marked as active his her identification code will not be listed in for example the Referrer selection box for a semen sample e Password his her email address Note only the Administrator can assign a password to a referrer and by doing so grant access to the AutoSperm software and database These referrers can login using their identification code as login name e Repeat password repeat the same password Click OK to save the changes Calculate volume Command Tools Calculate volume Description Calculates the volume in eL needed in order to obtain a pre specified preparation depth zm and given the length mm and width mm of the cover slide Test tablet Command Tools Test tablet Description This procedure allows to test the tablet The position of the cursor on the tablet is shown When you press a button the corresponding button in the dialog box is highlighted Test tablet O 2 Calibration Command Tools Calibration Description Allows to calculate the correction factor for measurement of velocity Zoom drawing tube The black template with the white grid is positioned under the transparent plastic cover of the digitizing tablet so that the grid is in the
5. the changes that you have made are saved If you do not want your recent changes to be saved click Cancel Tablet Tablet e Tablet type select the type of tablet connected to the computer The current version of AutoSperm supports the SummasSketch III DrawingBoard III and DrawingBoard VI tablets e Communication port select the number of the RS 232 communication port the tablet is connected to usually number 1 e Click the Test button to test the tablet with the two preceding settings e Velocity correction factor Although this value can be set manually it should be calculated using the corresponding procedure in the Tools menu e Size of tablet square side in um The size of the square on the digitizing tablet The recommended value is 100 em If the zoom of the drawing tube cannot be adjusted so that the size of the square corresponds with 100 em the closest size that can be obtained should be used This size must not be a rounded number like 150 or 200 the exact size of the square as measured with a micrometer slide must be entered Sounds e Computer beep pitch and time These numbers define the Beep sound produced by the computer This sound is hardware dependent Recommended values are 1500 Hz for pitch and 20 msec for time Buttons e OK saves the options and closes the dialog box e Cancel closes the dialog box without saving Remember when you switch from one tab to another the changes that you have made are save
6. um sec 24 7 5 4 23 5 Start analysis F9 Linear Velocity um sec 21 6 5 5 72 3 Linearity Index 86 8 10 2 39 1 Software features Measurements e Sperm concentration e Average velocity linear velocity and linearity index e Motility grading in agreement with the World Health Organization Laboratory Manual for the Examination of Human Semen and Semen Cervical Mucus Interaction Cambridge University Press 4th ed 1999 e Concentration of grade a b c and d motile spermatozoa Database layout e 2sample groups patients laboratory e subgroups can be defined for both groups e 3 analyses per sample additional analysis user defined e users database administrator technicians referrers Access control e Administrator Manages the AutoSperm software Can create and delete technician and referrer accounts Can delete samples from the database Can grant login rights to referrers by providing a password Cannot perform semen analyses e Technician Can perform semen analyses Can create Referrers e Referrer Can retrieve results of own patients in the patients group only Reporting e Export selected samples to Excel or the MedCalc statistical software package e Calculation of monthly averages mean and median of sperm concentration grade a and grade a b motility as well as linear velocity for quality control purposes Averages can be exported to Excel or MedCalc How to setu
7. SOLDriver SOL Native Client SOLServer SQLEXPRESS SOLWindowsAuthentication 1 SOLUserName SOLPassword Example 2 autosperm ini with redirection SOLIniFile path to autospermsql ini file should be absolute AutoSpermSQLIniFile autospermsgl ini Example file autospermsal ini SOLConnection SOLDriver SOL Native Client SOLServer SQLEXPRESS SOLWindowsAuthentication 1 SOLUserName SOLPassword Download AutoSperm CS is available as a separate download on http www autosperm com download AutoSperm cgi module The autospermcgi exe executable can be placed in the cgi bin folder of your web server and can be integrated in web page scripts allowing consultation of AutoSperm data using a web interface Command line parameters The program s command line parameters are r reference lists sample dates and numbers s samplenumber returns report for samplenumber 40 i Logie specifies logfile h returns Content type text html header y displays copyright and version information displays help SQL Connection data The connection with the SQL database is configured using the autosperm ini file Example 1 autosperm ini with SQL connection data SOLConnection SOLDriver SOL Native Client SOLServer SQLEXPRESS SOLWindowsAuthentication 1 SOLUserName SOLPassword Example 2 autosperm ini with redirection SOLIniFile path to autospermsql ini file should be absolute AutoSpe
8. center of the microscopic visual field Adjust the zoom of the drawing tube so that the superimposition of the square on the microscope slide corresponds exactly to 100 x 100 micrometer zm Use a micrometer slide for verification see Figure If the zoom of the drawing tube cannot be adjusted so that the size of the grid corresponds with 100 em it should be set as close to 100 em as possible and the size of the grid as measured by the micrometer must be entered in the Tablet section of the Options panel p 29 Calculation of correction factor Follow the instructions shown in the dialog box place the cursor on the different corners of the grid as illustrated on the screen and press a button on the cursor Having done that the computer calculates the correction factor Click OK to save the newly calculated correction factor Click Cancel to keep the previous correction factor 28 Options Command Tools Options Description Allows to customize the AutoSperm software You can switch between the following tabs Settings analysis settings Tablet tablet settings and sound options Lists subgroups lists referrer titles list Additional analyses configuration of the additional analysis input panel Text macros define text macros Options miscellaneous options When you switch from one tab to another the changes that you have made are saved in an options file Settings and Text Macros or in the database L
9. including but not limited to implied warranties of satisfactory quality and fitness for particular purpose with respect to the software and the written materials that accompany the software MedCalc Software and its suppliers shall not be liable for any damages whatsoever including without limitation damages for loss of business profits business interruption loss of business information or other pecuniary loss arising out of the use or inability to use the software In any case MedCalc Software s entire liability under any provision of this Agreement shall be limited to the amount actually paid by you for the software This license agreement may change without notice The most recent version which replaces any previous version is always available from http www autosperm com license AutoSperm is a registered trademark of MedCalc software 33 Admin menu To gain access to the Admin menu you have to login as the AutoSperm administrator Recode Subgroup Command Admin ReCOde Subgroup Description Allows to recode the sample subgroups in the database Required input e Sample group select the Patients or Laboratory sample group e Old subgroup code select the subgroup code from the list of all subgroup codes present in the database for the selected sample group e New subgroup code select the new subgroup code from the list defined in the Options panel Lists section p 30 Click OK to replace all occurrences
10. index n Conditionally writes the text Mean if analysis n has been performed Conditionally writes the text SD if analysis n has been performed Conditionally writes the text Median if analysis n has been performed Shows a histogram for Velocity Shows a histogram for Linear Velocity Shows a histogram for Linearity Index o ue There may be 20 additional analyses fields for each sample Replace nn in the field name with 01 02 20 E g ADDINFO 01TXT is the 1st description field and ADDINFO 01RES is the 1st result field ADDINFO 12TXT is the 12th description field and ADDINFO 12RES is the 12th result field ADDINFO nnTXTS ADDINFO nnRES ADDINFO INFORMATION Validation fields VALIDATEDS VALIDATIONDATE VALIDATEDBY VALIDATIONCOMMENTS Miscellaneous fields PROGRAMPATH COPYRIGHT Description Result Additional information Yes if all analyses of the sample have been validated No otherwise Date of validation Validated by Validation comments The AutoSperm program path e g c Program Files AutoSperm Displays copyright information This field must be included in the report template or the report will fail to print 38 How to integrate AutoSperm in hospital infrastructure To integrate AutoSperm in existing hospital IT infrastructure you can e place the AutoSperm database on a central server running SQL Server 2000 SQL Server 2005 or SQL
11. of the old subgroup code with the new subgroup code Collect Subgroups from Database Command Admin Collect Subgroups from Database Description Allows collect the subgroup codes from the database Required input e Sample group select the sample group Patients or Laboratory Click OK to scan all samples belonging to the selected sample group all subgroup codes will be collected into the subgroup list which can be edited in the Options panel Connect to SQL server Command Admin Connect to SQL server Description Use this dialog to view or specify options when connecting to the Microsoft SQL Server 34 Required input Driver select SQL Native Client or SQL Server Server select the server to connect to When SQL Server 2005 Express Edition is installed on your local PC enter sqlexpress If you are connecting to SQL Server 2005 Express Edition on a remote computer use the computer name followed by sqlexpress Authentication two authentication modes are available 1 Windows Authentication allows to connect through a Windows user account 1 SQL Server Authentication when a user connects with a specified login name and password from a non trusted connection SQL Server performs the authentication itself by checking to see if a SQL Server login account has been set up and if the specified password matches the one previously recorded If SQL Server does not have a login account set authentication fails
12. server If you are using SQL Server 2005 Express complete the dialog box as shown here If you are using SQL server 2000 or 2005 ona remote machine on the network consult your network administrator for connection details The database autosperm5 does not exist Click OK to create it You can now login to the program for the first time Use ADMIN for both login and password Setup final steps Test the hardware After the digitizing tablet has been connected to the computer you complete the following steps e Select Options in the Tools menu p 29 e Click the Tablet tab e Select the tablet type SummasSketch III DrawingBoard III or DrawingBoard VI e Select the communication port the tablet is connected to usually 1 or 2 e Click the Test button see Test tablet p 27 Calibrate the system See Calibration for details p 28 Create AutoSperm users Since as an administrator you cannot perform semen analyses in the AutoSperm system you must create one or more AutoSperm users technicians To create a new user e Select Users in the Tools menu e Click the New button e Complete the panel as explained on User data p 25 Counting procedure Procedure for DrawingBoard VI tablet motile cell f S immotile cell E end square F After liquefaction an aliquot of 11 5 L of thoroughly mixed either undiluted or diluted fresh semen is placed on a microscope slide and covered with a standard cove
13. 237 241 Kamtchouing et al Etude comparative entre trois m thodes d analyse de sperme Andrologie 1991 1 61 64 The AutoSperm method allows objective semen analysis using inexpensive equipment and fully validated technology The following elements of semen analysis are accurately assessed sperm concentration motility motility grading in agreement with the World Health Organization Laboratory Manual for the Examination of Human Semen and Semen Cervical Mucus Interaction Cambridge University Press 4th ed 1999 concentration of grade a b c and d motile spermatozoa as well as motility characteristics including velocity linear velocity and linearity The data are acquired in less than 5 minutes per semen sample The database can be configured to contain data of additional analyses The method has been proven accurate reproducible and clinically useful Hinting et al Validation of a single step procedure for the objective assessment of sperm motility characteristics Int J Androl 1988 11 277 287 Hinting et al Capacity of objectively assessed sperm motility characteristics in differentiating between semen of fertile and subfertile men Fertil Steril 1988 50 635 639 Principle of the AutoSperm method A zoom drawing tube is attached to the microscope A digitizing tablet is placed next to the microscope The zoom of the drawing tube is adjusted so that the sides of the grid correspond with 100 em in the microscopi
14. 29 e Dilution if the semen used for analysis is diluted then you must enter the proportion of semen and the proportion of diluent If the semen is not diluted enter the value 1 for proportion of semen and the value O for proportion of diluent The default dilution is entered in the Options section of the program p 29 e Time after ejaculation the time interval between time of ejaculation and the time of analysis i e the current time is calculated by the program If the value is not displayed or is not correct you can enter the correct time interval between ejaculation and semen analysis Analysis Click the Start analysis button to start the counting procedure see p 12 Results e The number of cells analyzed e The number of motile cells counted e Sperm concentration in 10 mL e Proportion of spermatozoa with grade a b c and d motility in and concentration of spermatozoa with grade a b c and d motility in 10 mL A spermatozoon is classified as grade a if it has a rapid linear progressive motility with linear velocity in excess of the lower limit selected in the Options box grade b if it has a slow or sluggish linear or non linear movement such a spermatozoon has a linear velocity below the higher limit selected in the Options box but a velocity in excess of the lower limit selected in the in the Options box grade c if it has a non progressive motility its velocity is b
15. AUTOSPERM Computer Assisted Semen Analysis manual MedCalc Softw are Microsoft Partner this page intentionally left blank MedCalc Software bvba All rights reserved No part of this package neither the documentation nor the software may be reproduced stored in a retrieval system or transmitted in any form by electronic mechanical photocopying or any other means without the prior written permission of the publisher The author and the publisher make no representations or warrants with respect to the software and the documentation and specifically disclaim any implied warranties of fitness for any particular purpose Microsoft Excel Windows and Windows Vista are trademarks or registered trademarks of Microsoft Corporation in the United States and or other countries AutoSperm and MedCalc are registered trademarks CERTIFIED FOR Windows Vista Version 5 AUTOSPERM Copyright O 1989 2009 MedCalc Software bvba MedCalc Software Broekstraat 52 B 9030 Mariakerke Belgium info medcalcsoftware com www medcalcsoftware com www autosperm com this page intentionally left blank Contents INTOdUCION cule 1 Principle Of Ihe AUTO Sperm MEMO lia dto nO Sola O 1 A O O ascends E E 3 Fow to Setup the AUTOS pelt SYSlOM sisiaseasiavsices tow scerssdetisatbanatcosnausidvs aid 5 AUTO SPerm Setup IMMOCUCTON resis additional 5 NAS a e o o ene 6 How to connect the DrawingBoard VI tablet
16. Additional information box Buttons e Print print a complete report e OK saves the data in the database and closes the dialog box e Cancel closes the dialog box without saving the data in the database Tips When you click Print the report will immediately be printed to the default printer If you want to select a printer first press Ctrl P or function key F11 Remember that when you switch from one tab to another the changes that you have made are saved in the database If you do not want your recent changes to be saved click the Cancel button Validation Sample 06 0356 Sample Analysis ii Analysis 2 Analysis 3 Additional analyses Validation Validated Validation date 27 12 2006 Validated by JOHN Comments Oligozoospermia Required input e Click the Validated check mark when the results are complete and have been validated In addition there is space to enter some final comments Buttons e Print print a complete report e OK saves the data in the database and closes the dialog box e Cancel closes the dialog box without saving the data in the database Tips When you click Print the report will immediately be printed to the default printer If you want to select a printer first press Ctrl P or function key F11 Remember that when you switch from one tab to another the changes that you have made are sav
17. E A E E E E a ih ee ad El Rad a Detaled E HA E o 5 10 15 20 25 30 35 40 45 a 18 Buttons e Print print a complete report e OK saves the data in the database and closes the dialog box e Cancel closes the dialog box without saving the data in the database Tips When you click Print the report will immediately be printed to the default printer If you want to select a printer first press Ctrl P or function key F11 Remember that when you switch from one tab to another the changes that you have made are saved in the database If you do not want your recent changes to be saved click the Cancel button Additional analyses Sample 06 0356 Sample Analysis 1 Analysis 2 Analysis 3 Additional analyses Validation Volume mL pH Consistency Appearance Normal La j 5 pa co Morphology normal Morph abnormalities head abnormalities Agglutination grade f Round cells mill mL Additional information WEC 56 MAR test IgG pos i MAR test IgA pos Required input This dialog panel can been configured by the AutoSperm administrator e Inthe longer input fields you can enter text the shorter input fields require numeric input e In all input fields you can enter a sign indicating that this information is not yet available and needs to be completed e Enter possible other comments and information in the
18. NCE S NOTES EJACULATIONDATES EJACULATIONTIME S ABSTINENCE Referring doctors fields Sample number Patient name Patient date of birth Patient record number Partner name Partner date of birth Subgroup code Reference Notes Ejaculation date Ejaculation time Abstinence days There may be 2 referring doctors for each sample Replace n in the field name with 1 or 2 E g REFERRER1 NAME is the name of the first referrer and 4REFERRER2 NAME is the name of the second referrer REFERRERn NAME REFERRERn FIRSTNAME REFERRERn TITLE REFERRERn ADDRESS REFERRERn PHONE REFERRERn MOBILE REFERRERn FAX REFERRERn EMAIL REFERRERn LICENSE Semen analysis fields Referrer name Referrer first name Referrer title Referrer address Referrer phone Referrer mobile phone Referrer fax Referrer email Referrer license number There may be 3 semen analysis for each sample Replace n in the field name with 1 2 or 3 E g AN1 CONCENTRATIONSS is the sperm concentration for the 1st semen analysis and AN2 CONCENTRATION is the sperm concentration for the 2nd semen analysis ANn ANALYSIS ANn ROOMTEMPERATURE ANn PREPARATIONDEPTH ANn DILUTIONSEMENS ANn DILUTIONDILUENT ANn TIMEAFTEREJACS ANn TOTALCELLS ANn NMOTILES Analysis short description Room temperature Preparation depth Dilution proportion semen Dilution proportion dilu
19. Server 2005 Express Edition e adapt the autosperm dll library so you can exchange data between the AutoSperm database and hospital databases e use the AutoSperm CS program to allow consultation of the AutoSperm data on different terminals connected to the central system using a graphic user interface GUI e use the autospermcgi exe program in web pages that allow data consultation using a web interface This is illustrated in the following figure AutoSperm system A Server autosperm dil allows a data exchange with ds autospermegi integrates In other databases aa web pages allowing data consultation using web inferface AutoSperm CS allows data T i consultation using graphic user interface GUI _ Ej See also AutoSperm setup introduction p 5 autosperm dll library The autosperm dll library can be used to exchange data between the AutoSperm database and hospital databases A complete Visual Studio 2005 project is available from the AutoSperm download site which contains a framework of functions that hook into the AutoSperm program In summary the dll library contains the following functions e The function InitAutoSpermDLL is called when the AutoSperm program is started In this function you can implement the code required to establish a connection with an external database In addition this function is used to notify AutoSperm of the date format you will be using dd mm yyyy mm dd yyyy
20. above Use the Browse button to locate the file on your system Sample group select the sample group Patients or Laboratory for which you are importing data Import code enter the import code provided to you by MedCalc Software not required when importing data from AutoSperm version 4 Click OK to import the data Note the import procedure will not overwrite existing sample data which have the same sample number 35 Rebuild Indexes Command Admin Rebuild Indexes Description This command allows to rebuild the AutoSperm database indexes 36 How to customize the printed report The sample report created by AutoSperm is based on a template file named reporttemplate htm which is located in the AutoSperm program directory typically c program files autosperm This file is a standard HTML file which you can modify Before modifying the file make a backup copy first The file must be saved as a UNICODE text file You can place your laboratory name and address in the template and possibly add images such as a laboratory logo The template can contain a large number of fields listed below which at the time of printing are replaced with their corresponding values For example the field 5PATIENTNAME will be replaced with the patient name Patient and sample identification fields SAMPLENUMBER PATIENTNAMESS PATIENTDOB RECORDNUMBER S PARTNERNAMES PARTNERDOB SUBG ROUP REFERE
21. and the user receives an error message User name enter the user name to connect with This option is only available if you have selected to connect using SQL Server Authentication Password enter the password for the login This option is only available if you have selected to connect using SQL Server Authentication Click OK to connect to the selected server Import datafile Command Admin Import datafile Description Allows to import data from previous versions of MedCalc Before you can import the data a conversion program should be executed depending on the previous AutoSperm version number Instructions for Version 3 6 Download the conversion program convert3 exe from the AutoSperm download site http www autosperm com download Save the convert3 exe file in the folder where AutoSperm 3 6 is installed in e g c autosp Execute convert3 exe lt is recommended to contact MedCalc software before you import the conversion file into AutoSperm 5 Specific procedures may be needed to optimally import the old data into the new version of the software Instructions for Version 4 Download the conversion program Converter_setup exe from the AutoSperm download site http www autosperm com download Install and execute the conversion program Required input Import from enter the location and name of the import file e g c autosp convert5 txt that was created by one of the conversion programs mentioned
22. as login name Name the user s name Fist name the user s first name Phone phone number Mobile mobile phone number E mail his her email address Password his her email address Repeat password repeat the same password Active mark the user as active If the user is not marked as active he she will not be able to login and the identification code will not be listed in for example the Technician selection box when performing a semen analysis Note only the Administrator can change the active status of a user Click OK to save the changes Referrers list Command Tools isos Referrers Description Allows to add referrers or update a referrer s data Required input The referrers are listed in the main box of the dialog panel Note List active only if this option is checked then only the referrers that are marked as active are listed Click New to add a new referrer to the system Click Edit to make changes Click Close to close this dialog box the Administrator can assign a password to a referrer and by doing so grant access to the AutoSperm software and database There referrers can login using their referrer code as login name and they can update their own data including their password Referrer data Required input Identification code the identification code for this referrer Name the referrer s name Fist name the referrer s first name Title select for example Dr or Prof Dr
23. ate of birth Partner name Date of birth 06 0356 650325 023456 01 12 1970 30 04 1973 The samples that satisfy the search criteria are listed in a grid To view the results of one sample click on that sample in the grid and click Open or double click on the sample to print a report click Print To print multiple reports select different samples in the grid and click Print To select multiple samples you click on different samples while holding down the Ctrl key Click Back to change the selection criteria Tip You can change the column order by dragging the header to a new location Print Journal Command Reporting Print Journal Description Allows to print a journal of samples that were analyzed on one day or in a date range Dialog box Print Journal Sample group v Ejaculation date 07 02 2007 to fs Cancel Enter possible search criteria e Sample group select the Patients or Laboratory sample group 22 e Ejaculation date date of ejaculation Enter one date or two dates to specify a date range Click Print to print a list per day of samples that were analyzed in the given date range Samples Requiring Validation Command Reporting i Samples Requiring Validation Description Allows to search for samples requiring validation Dialog box Search samples requiring validation Ejaculation date 02 01 2007 95 01 2007 Enter possible search criteria e Sample group sele
24. c field The technician observes the spermatozoa in the visual field together with the cursor on the grid A motile cell is registered by following the movement of the cell with the cursor for a short period of time whilst keeping a button depressed An immotile cell is registered by pressing a different button on the cursor After all cells in the grid have been registered a third button is pressed and analyis continues on a different visual field The AutoSperm program analyses the data from the digitizing tablet semen analysis Sample Sample number 06 0356 Patient name Adami Analysis Native sample Count Grade A Grade B Grade C Grade D Total cells Squares Cancel When a sufficient number of spermatozoa have been registered a summary report is displayed sample 06 0356 Sample Analysis 1 Analysis 2 Analysis 3 Additional analyses Validation Analysis Native sample Results Velocity Graph Linear Velocity Linearity Index Technician JOHN M Total cells counted 53 A Motile cells 32 Room temperature C 22 Sperm concentration mill mL 33 1 Preparation depth um L Grade A motility 32 10 6 mill mL Dilution Semen Grade E motility 5 28 9 4 mill mL z E Grade C motility 2 0 0 0 mill mL Dduent ssa Grade D motility 36 40 13 1 mill mL Time after ejaculation jo 1 09 Mean SD Median Velocity
25. ct the Patients or Laboratory sample group e Ejaculation date date of ejaculation Enter two dates to specify a date range Click Search to obtain a list of samples satisfying the search criteria and still requiring validation Search results Search results Sample number Record number Patient name Date of birth Partner name Date of birth Sample dat 650325 023456 01 12 1970 30 04 1973 15 12 2006 The samples in the selected date range still requiring validation are listed in a grid To view the results of one sample click on that sample in the grid and click Open or double click on the sample 23 To batch validate multiple reports select the samples in the grid and click Validate selected To select multiple samples you click on different samples while holding down the Ctrl key Click Back to change the selection criteria Tip You can change the column order by dragging the header to a new location Monthly Averages Command Reporting Monthly Averages Description Allows to calculate monthly averages both mean and median for quality control purposes Dialog box Enter possible search criteria e Sample group select the Patients or Laboratory sample group e Subgroup select a possible subgroup code e Ejaculation date enter two dates to specify a date range e Sperm concentration enter a minimum value for soerm concentration e Calculate per months enter the number of months 1 to 12 to po
26. d If you do not want your recent changes to be saved click Cancel Lists ot Required input e Subgroups define the sample subgroups for both sample groups Enter one subgroup name per text line For example for the sample group Patients you can enter PATIENT CRYO For the sample group Laboratory you could enter DONOR CANDIDATE DONOR TEST 30 e Referrer titles titles Dr Prof Dr etc that can be assigned to referrers Buttons e OK saves the options and closes the dialog box e Cancel closes the dialog box without saving Remember when you switch from one tab to another the changes that you have made are saved If you do not want your recent changes to be saved click the Cancel button Additional analyses Required input e Inthis panel you can define up to 18 additional data fields for each semen sample For each field you can specify whether the input needs to be a number or can be text Buttons e OK saves the options and closes the dialog box e Cancel closes the dialog box without saving Remember when you switch from one tab to another the changes that you have made are saved If you do not want your recent changes to be saved click the Cancel button Text macros Required input e In this panel you can define 10 text macros A text macro is a short text that is assigned to the combination of the Control key and a key on the numeric keypad Buttons e OK saves the options and closes th
27. e dialog box e Cancel closes the dialog box without saving Remember when you switch from one tab to another the changes that you have made are saved If you do not want your recent changes to be saved click the Cancel button Miscellaneous options Required input e Lock sample data when results have been validated when this option is checked the sample and semen analysis data cannot be changed when the sample has been marked as validated e Show validation status in sample dialog title when this option is checked the sample dialog box will display the text validated next to the sample number when the sample has been marked as validated e Graphs show histograms in a histogram bars represent absolute number of cases relative frequency distributions in a relative frequency distribution bars represent the relative number of cases expressed as a percentage 31 e Graphs use equal Y scaling per variable for different analyses in the same sample e g when 2 analyses have been performed in the same sample both graphs for Linear Velocity will have the same Y scaling which facilitates comparison of the two graphs Buttons e OK saves the options and closes the dialog box e Cancel closes the dialog box without saving Remember when you switch from one tab to another the changes that you have made are saved If you do not want your recent changes to be saved click Cancel AutoSperm help Command Help Au
28. ed in the database If you do not want your recent changes to be saved click the Cancel button Search Sample Command Sample Search Sample Description Allows to search for a sample using one or more search criteria 20 Dialog box Search sample Sample group Sample number Patient record number Patient name Date of birth Partner name Date of birth Subgroup Ejaculation date Referrer Reference Enter one or more search criteria For sample number record number patient and partner name you can enter the first characters only E g if you enter sm the program will locate all names beginning with sm such as Smith Smythers etc Sample group select the Patients or Laboratory sample group Sample number sample number Patient record number a record number that identifies the patient e g hospital record number Patient name the patient s name Date of birth the patient s date of birth Partner name the name of the partner Date of birth the partner s date of birth Subgroup select a possible subgroup code Ejaculation date date of ejaculation Enter two dates to specify a date range Referrer select the code of the referring doctor Reference enter a reference number Click Search to obtain a list of samples satisfying the search criteria Click Reset to blank all the input fields 21 Search results Search results Sample number Record number Patient name D
29. el appears Click Next Select the locations of different shortcuts to the AutoSperm program Click Next Select the location of the AutoSperm program files It is recommended to accept the default value Click Next Toe rt wa Ready to Install The Setup Wizard is ready to begin the Typical installation The installation program is now ready to install AutoSperm Click Install Back ig AutoSperm Setup Please wait while the Setup Wizard installs AutoSperm This may take several Status SEE SEE REESE SEER REE REE EERE EERE EEE EEE EEE EEE EEEEE This panel shows the installation status i AutoSperm Setup lie o A Y Completing the AutoSperm i i Setup Wizard Click the Finish button to exit the Setup Wizard Installation is complete The Launch AutoSperm option is checked by default You are now ready to run AutoSperm for the first time Click Finish Launch AutoSperm 1 lt Back Finish Cancel Running the program for the first time The first time you run the AutoSperm program you have to complete the following steps AutoSperm Connect to server Driver Server ASQLEXPRESS Authentication Windows Authentication O SQL Server Authentication User name Password AutoSperm Create database T Database AUTOSPERMS does not exist Create new database AutoSperm 5 Login ADMIN 10 Provide connection data for the SQL
30. elow the higher limit selected in the Options box grade d if the spermatozoon is immotile e Mean standard deviation and median of velocity linear velocity and linearity index of grade a b and c motile cells only e Velocity is the total distance covered by the spermatozoa per unit of time and is expressed in em sec e Linear Velocity is the shortest distance between the first and the last point of measurement calculated per unit of time and is expressed in em sec note that this is the theoretical definition AutoSperm uses a more advanced method for estimating linearity of cell movement see p 42 e Linearity Index is the quotient of linear velocity divided by velocity multiplied by 100 Graphs Click on Velocity graph Linear Velocity or Linearity Index to obtain a histogram for the corresponding parameter Results Velocity Graph Linear Velocity Linearity Index Linear Velocity um sec 12 Bree E Eh E E E E E us E E 10 E E E ARA Behe Behe Sees E E E E E E k E E br E E E A A la m y EPEE EEEE A A e gt ES Er E E Bree EE E E E eee Por A ER ECO ih ee nota ch e e E ER Eee tie tie tie Echo Eh E EA EA RAR E AAA AA AAA ee eee eee eee E eee ee ee ee chet E E a Ek E E E E E
31. ent Time after ejaculation Total number of cells counted Total number of motile cells counted 37 ANn NSQUARES ANn CONCENTRATION ANn GRADEA ANn GRADEB ANn GRADEC ANn GRADED ANn GRADEACONC ANn GRADEBCONC ANn GRADECCONC ANn GRADEDCONC ANn VELOCITYMEANS ANn VELOCITYSD ANn VELOCITYMEDIANS ANn VELOCITYN ANn LINVELOCITYMEAN ANn LINVELOCITYSD ANn LINVELOCITYMEDIANS ANn LINVELOCITYN ANn LININDEXMEAN ANn LININDEXSD ANn LININDEXMEDIAN ANn LININDEXN ANn STR_MEANS ANn STR_SD ANn STR_MEDIAN ANn GRAPHVELOCITY ANn GRAPHLINVELOCITY ANn GRAPHLININDEX Additional analyses fields Number of squares counted Sperm concentration grade a motility grade b motility grade c motility grade d motility Concentration of grade a Concentration of grade b e motile spermatozoa motile spermatozoa motile spermatozoa motile spermatozoa Concentration of grade c Concentration of grade d Mean velocity Standard Deviation for velocity Median velocity Number of cells used to calculate mean velocity n Mean linear velocity Standard Deviation for linear velocity Median linear velocity Number of cells used to calculate mean linear velocity n Mean linearity index Standard Deviation for linearity index Median linearity index Number of cells used to calculate mean linearity
32. igitizing tablet attached to it and which connects to a SQL server on a remote machine in the network This offers the advantage that using the AutoSperm program or a special version of the software which is AutoSperm CS the data can be accessed simultaneously by different users Required e A PC which will serve as an AutoSperm counting machine with Windows 2000 Windows XP or Windows Vista PC with Windows XP SP2 is recommended and one free serial port RS 232 to connect the AutoSperm digitizing tablet e Connection to a SQL server the AutoSperm program requires full rights e On other PCs in the network the AutoSperm software can also be installed or a special version of the software AutoSperm CS which allows data consultation only See also How to integrate AutoSperm in hospital infrastructure p 39 Database maintenance For 15000 semen analyses the size of the SQL autosperm5 mdf data file is about 115 MB This file will need to be backed up according to the institution policy daily recommended The SQL database and tables will be created and maintained by the AutoSperm system no manual interventions are required Hardware setup The hardware for the AutoSperm system is shown in the following picture YY F p 1 drawing tube i A zoom drawing tube is attached to the microscope The digitizing tablet is placed as close to the microscope as possible The black template with the white grid is p
33. ists and Additional analysis lf you do not want your recent changes to be saved click Cancel Click OK to close and save the data Analysis settings Default settings Default preparation depth um The default preparation depth is the default value for the semen preparation chamber depth Preparation depth is initialized as 20 This value will automatically be displayed in the semen analysis dialog box Default dilution If you preferentially work with diluted semen you can enter the proportion semen and proportion diluent that will appear automatically in the semen analysis dialog box Analysis Delay time msec The hand being slow when starting to follow the sperm movement the initial observations for every motile spermatozoon will be inadequate The delay time expressed in milliseconds is the time fraction of initial observations that will be ignored for velocity calculations This value is set by the manufacturer and should not be changed by the user Track time per cell excl delay msec The maximum track time per cell is the time required to obtain adequate assessment of sperm motility characteristics This value is set by the manufacturer and should not be changed by the user Minimum track time excl delay msec If a cell has a high velocity and moves out the visual field before the maximum track time is passed the user should release the button of the cursor If the minimum track time as defined on this line is
34. mirer O Veer renee ee eete ree cee 32 A Sek ed eka eset Meee ieee ele te retell cet de cent tes 32 FACGISI A ee pier lated ea le aide ala beetle Sina lla Lies adsl ad tare iia 32 ABDOU FRU OSG MINN aa 33 Software license ACME a le 33 AamIN NENU ca A A E a 34 EE a a a a RE or 34 Collect Subgroups from Database ccccecccceececeeeeseeeeeaeeeeeeeeeaeeeeseeeeeseeeeseeeeeseeesseeeeseesesaeesseeeesaes 34 COMME CUO A E E NE E EE 34 mpor CAL ATC stas lla iii 35 Rept INGE XE Sn aua OR 36 row to customize the Printed TODO iii da sian 37 How to integrate AutoSperm in hospital infraStructure ococcocconconnonnonconconconconconncnnnrnnnoncnarnrnnrnannnos 39 vea A o o 39 AUS A AEA E EAEAN AEAN E N A A EEA 40 PAULO SDE ed Mod le ssa a a e ea a a a cared a e a A 40 Appendix A Cal CUASI 42 Introduction Semen analysis is the cornerstone of functional evaluation of the male reproductive system and fertility In most laboratories semen analysis is performed with subjective methods and results depend on the skillfulness and judgment of the observer Up to now there has not been any widely available technology for reliable assessment of motility characteristics Sophisticated and costly equipment has been developed to establish objective sperm analysis using image analysis techniques which are time consuming expensive and relatively inaccurate Comhaire et al Objective semen analysis has the target been reached Hum Reprod 1992 7
35. nt in the large square without scanning through the small squares If soerm concentration is high it is suggested either to reduce chamber depth or to dilute the sample In the former case only 8 6 EL of semen is placed under a 24 x 24 mm coverslip resulting in a chamber depth of 15 em 8 6 eL divided by 576 mm is equal to 0 015 mm It is however not recommended to reduce the chamber depth bellow 15 em since this will hinder the free movement of sperm and artificially reduce linearity Alternatively the sample can be diluted before being evaluated Dilution is preferentially done by means of cell free seminal plasma of the particular semen sample obtained by centrifugation The reduction of chamber depth or the dilution factor must be recorded and the system automatically calculates sperm concentration taking into account these factors Remember e Change the visual microscopic field randomly Do not search for motile cells if motility is low or do not search for cells if concentration is low e Record every square changing to another microscopic field must always be recorded by pressing button 4 even if no cells were present in the square e When you are following a cell but it moves out of the visual field simply release button 1 of the cursor and continue the analysis If the cell was followed during a sufficient period of time pre set in the settings and options menu it will be taken into account for analysis Menu overview
36. ol results When you do not have enough samples less than 20 per month it is advised to pool results from 2 or more months Click OK to calculate mean and median values for sperm concentration grade A grade A B motility and linear velocity per month in the selected date range Report After the software has performed the calculations the following report is displayed Monthly averages Month Concentration Grade A B Linear Velocity n Mean Median n Mean Median n Mean Median 94 35 4 23 9 69 g 41 6 44 1 87 624 0 23 0 589 36 2 25 0 61 i i 44 6 47 3 T7 22 3 22 1 76 37 6 30 9 FO 46 3 43 2 6 7 23 4 22 1 79 31 6 21 3 fa Fa 41 4 42 0 PO 23 0 24 6 67 39 5 36 4 61 i 44 4 49 0 57 25 4 23 9 63 38 6 28 9 fa 73 40 1 41 5 fa 24 9 25 2 61 23 1 13 2 53 i 36 3 37 3 50 223 28 5 60 29 5 24 5 4 l 40 1 45 0 67 24 2 23 0 ar id 31 7 70 Fl 44 6 42 3 FS 21 1 19 7 37 9 20 8 41 5 44 0 21 21 8 63 43 9 20 8 69 45 4 44 5 68 25 3 24 4 64 53 4 42 4 62 y y 44 6 45 1 56 24 0 22 3 1 2 3 A 5 6 Fl 8 g Export Click Print to print the report or Click Export to export the data to an Excel xls file or a MedCalc file mc1 file 24 Tip When you click Print the report will immediately be printed to the default printer If you want to select a printer first press Ctrl P or function key F11 Export Data Command Reporting Export Data Description Allows to export the data from the AutoSperm database as an Excel file or a MedCalc
37. or yyyy dd mm e The function AutoSpermDLLGetldentification is called when the user has entered a Patient record number in the Sample dialog box and moves the input focus to the next field by pressing the Tab key for example In the AutoSpermDLLGetldentification function you can implement the code to read the patient and partner names and dates of birth from the hospital database and return these data to the AutoSperm program e The function AutoSpermDLLSaveData is called when sample data have been changed The sample data are passed as a parameter In this function you can implement the code needed to save the AutoSperm data into the hospital database 39 e The function UniInitAutoSpermDLL is called when the AutoSperm program is closed In this function you can implement the code required to close the connection with your external database Download A complete Visual Studio project autospermdll is available as a separate download on http www autosperm com download AutoSperm CS AutoSperm CS is a lightweight version of AutoSperm and can be used for data consultation only It does not allow to modify data This program does not need a product key or configuration by the user The connection with the SQL database is configured using the autosperm ini file SQL Connection data The connection with the SQL database is configured using the autosperm ini file Example 1 autosperm ini with SQL connection data SOLConnection
38. ositioned under the transparent plastic cover of the digitizing tablet so that the grid is in the center of the microscopic visual field How to connect the DrawingBoard VI tablet All cable connections are made at the rear of the tablet Turn off your computer and make sure the tablet power switch is in the off position Power switch Serial pot Power Cursor RS 232 Connect the cursor to the appropriate jack on the digitizer s Connector Panel The connector is keyed and will only fit the correct jack Do not force it Connect the RS232 serial cable to the serial jack on the Connector Panel The connector will fit only the correct jack Connect the 9 pin connector of the serial cable to the serial port on your computer Plug the power supply into an AC outlet Connect the power supply to the appropriate jack on the Connector Panel Turn the Power Switch on The light on the cursor will turn on AutoSperm software installation You install the software by executing the program autospermsetup exe During installation you will see the following panels ize AutoSperm Setup Welcome to AutoSperm Setup Wizard The Setup Wizard will install AutoSperm on your computer Click Next to continue or Cancel to exit the Setup Wizard Configure Shortcuts Create application shortcuts Create shortcuts for AutoSperm in the following locations Desktop Start Menu Programs folder After some initialization this Welcome pan
39. p the AutoSperm system AutoSperm setup introduction The AutoSperm system runs on any PC with Windows 2000 Windows XP or Windows Vista The program has been certified for Windows Vista The data are stored in a SQL database which can be SQL Server 2000 SQL Server 2005 or SQL Server 2005 Express free from Microsoft The database server can be installed locally or on a remote computer in the network If the server is on a remote machine then the database can be accessed simultaneously from different terminals using the same AutoSperm program or a special version of the software AutoSperm CS which allows consultation of the data only AutoSperm stand alone installation In this scenario the AutoSperm system is installed on one PC which is used as the counting machine with the AutoSperm digitizing tablet attached to it and which also holds the SQL database Required e PC with Windows 2000 Windows XP or Windows Vista PC with Windows XP SP2 is recommended and one free serial port RS 232 to connect the AutoSperm digitizing tablet e SQL Server 2000 SQL Server 2005 or SQL Server 2005 Express installed on the PC SQL Server 2005 Express is a free product from Microsoft and is included on the AutoSperm installation CD e The AutoSperm software takes less than 2 MB hard disk space AutoSperm in network environment In this scenario the AutoSperm system is installed on one PC which is used as the counting machine with the AutoSperm d
40. passed the cell is taken into account for analysis This value is set by the manufacturer and should not be changed by the user Minimum number of cells This is the minimum number of cells to be analyzed for adequate reproducibility Whenever a complete field has been analyzed indicated by pressing the end of field button on the cursor button 4 on a DrawingBoard VI tablet the number of cells already analyzed is compared with this value If the number of cells analyzed is lower than the set limit it is recommended to continue the analysis As soon as the number of cells analyzed exceeds the set limit a triple beep is heard and calculation starts Motility grade classification Grade a if linear velocity more than The number entered here sets the lower limit of linear velocity of grade a motile cells Cells with a linear velocity higher than this value will be classified as grade a The default value is 21 99 Em sec 29 e Grade c if velocity is less than The number here sets the higher limit of velocity of grade c cells The default value is 5 em sec Cells with a velocity lower than this value are classified as grade c Cells with a velocity higher than this value but with a linear velocity lower than the value in the previous line are classified as grade b cells Buttons e OK saves the options and closes the dialog box e Cancel closes the dialog box without saving Remember when you switch from one tab to another
41. rmSQLIniFile autospermsgl ini Example file autospermsal ini SOLConnection SOLDriver SOL Native Client SOLServer SQLEXPRESS SOLWindowsAuthentication 1 SOLUserName SOLPassword Download AutoSperm cgi is available as a separate download on http www autosperm com download 41 Appendix A Calculations Velocity um sec time n yy Linearity index 100 2 nxII Linear velocity cee ea MOEA um sec 100 42
42. rslip of 24 x 24 mm to give a uniform depth of 20 em 11 5 eL divided by 576 mm is equal to 0 02 mm or 20 em Alternatively an aliquot of 8 or 10 eL semen can be covered with a coverslip of 20 x 20 or 22 x 22 mm respectively Analysis is performed at room temperature between 20 and 24 C using the 40x objective Bring the sperm into focus Move the tablet to locate the grid in the center of the visual field Analysis starts from the small square A1 Figure 1 at the upper left corner of the large square grid Each sperm present in the small square must be classified Motile spermatozoa are tracked by moving the cursor on the digitizing tablet in such a way that the superimposition of the red light follows soerm movement as closely as possible While doing so button 1 of the cursor is pressed After a predefined period of time you hear a computer generated beep and you release the button Since it is impossible to track the lateral head movement the sperm has to be tracked at its midpiece The superimposition of the light point of the cursor on the microscopic field must follow the movement of the sperm midpiece as accurately as can be done by hand Spermatozoa presenting non progressive motility are identified by pressing button 1 of the cursor and leaving the cursor on the spot Immotile spermatozoa are recorded by pressing button 2 of the cursor lt is of the utmost importance to record each sperm which is present in a particular sq
43. s of the software license agreement By installing the software and entering you user name and personal product key you indicate acceptance of the software license agreement Grant of license The software is owned by MedCalc Software and is protected by United States copyright laws and international treaty provisions By purchasing a single user AutoSperm license you obtain a non transferable right to install the software on your desktop and laptop computer not serving as network servers and use the software non concurrently on these computers The software can be used on a network server when you purchase a network license for the required number of concurrent users You may not share sell rent or lease the software You may not transfer ownership of your license user name and product key You may not reverse engineer decompile disassemble or create derivative works from the software MedCalc Software reserves all rights not expressly granted to you in this license agreement Disclaimer of warranties Limitation of liability The software is designed and offered as a general purpose software not for any user s particular purpose You accept that no software is error free You are strongly advised to back up your files regularly To the maximum extent permitted by applicable law MedCalc Software disclaims all warranties conditions and other terms either express or implied whether by statute common law collaterally or otherwise
44. scription Allows to enter a new sample number Dialog box New sample Last used 06 0356 New sample number Cancel Select the sample group Patients or Laboratory The sample number last entered for the selected group is displayed as a reminder Enter the number of a new sample The program will not accept a number that has been used already Click OK to proceed and go to the Sample dialog Sample dialog The sample dialog box displays the sample number in its title bar You can switch between the following tabs Sample sample identification Analysis 1 a first analysis of the sample e g analysis of the native sample Analysis 2 a second analysis of the sample e g after soerm preparation Analysis 3 a third analysis of the sample e g survival test after 24 hours Additional analyses additional analyses and information Validation validation of results When you switch from one tab to another the changes that you have made are saved in the database If you do not want your recent changes to be saved click Cancel Click the Print button for a full printed report Click OK to close and save the data Tip When you click Print the report will immediately be printed to the default printer If you want to select a printer first press Ctrl P or function key F11 Sample Sample 06 0356 Sample Analysis i Analysis 2 Analysis 3 Additional analyses Validation Patient record number
45. see http www medcalc be file Dialog box Enter possible search criteria e Sample group select the Patients or Laboratory sample group e Subgroup select a possible subgroup code e Ejaculation date date of ejaculation Enter two dates to specify a date range e Referrer the code of a referring doctor Next complete the following fields e Export to enter the name of the Excel xls or MedCalc mc1 file If you enter the name of an existing file the file will be overwritten and the data it contains will be lost e Export what select the data groups you want to export Click OK to create an Excel file with the selected data from the AutoSperm database Users list Command Tools Description Allows to add users of the AutoSperm program or update user information Required input The users are listed in the main box of the dialog panel e List active only if this option is checked then only the users that are marked as active are listed e Click New to add a new user to the system Note only the Administrator can add new users e Edit View click Edit to make changes Note the Administrator can make changes for all users Other users can only update their own data including their password but can View the data of other users but not their password e Click Close to close this dialog box 25 User data Required input ID amp Login code the identification code for this user which will also serve
46. tion key F11 Remember that when you switch from one tab to another the changes that you have made are saved in the database If you do not want your recent changes to be saved click the Cancel button Analysis data Sample 06 0356 ren i i Sample Analysis 1 Analysis 2 Analysis 3 Additional analyses Validation Analysis Native sample Results Velocity Graph Linear Velocity Linearity Index Technican JOHN we Total cells counted 53 EF Motile cells 32 O tee E Sperm concentration mill mL 3 1 Preparation depth um Grade A motility 5 32 10 6 mill mL Dilution Semen Grade B motility 28 9 4 mill mL Grade C motility 5 0 0 0 mill mL Grade D motility 5 40 13 1 mill mL Time after ejaculation 01 09 Mean 5D Median Velocity m sec 24 7 5 4 23 5 Linear Velocity um sec 21 6 5 5 22 3 Linearity Index 86 8 10 2 89 1 Diluent Start analysis F9 Required input e Analysis a description of the analysis e g native semen after preparation etc e Technician the login name of the technician who performs or has performed the semen analysis e Room temperature enter the room temperature optional e Preparation depth this is the depth of the counting chamber derived from the known volume of semen and the dimensions length and width of the cover glass volume ul Depth um 1000 x Ee Tey e The default sample preparation depth is entered in the Options section of the program p
47. toSperm help Description Displays help You select AutoSperm help or press F1 to browse the comprehensive on line documentation In Help click one of the following tabs e Click the Contents tab to browse through topics by category e Click the Index tab to see a list of index entries and then either type a word or scroll through the list e Click the Find tab to search for words or phrases that may be contained in a Help topic What s New This help page lists the more important changes and new features in recent versions of AutoSperm The most recent additions and changes are listed at the top Register Command Help Register Description Allows to enter the registration data that you received from MedCalc Software Dialog box e User name the name of the software licensee e Product key the product key associated with the user name If you do not know your user name and product key contact MedCalc software Click OK to proceed 32 About AutoSperm Command Help About AutoSperm Description Displays program version specific information and copyright notice Dialog box This dialog box displays e the AutoSperm version number e copyright notice e license information e link to the AutoSperm website Click OK to close Software license agreement Use of the AutoSperm software is subject to the following software license Use the software only after you have agreed to abide by the terms and condition
48. uare at a given moment of time Do not wait for soermatozoa to enter the square nor skip any present spermatozoa as this will cause unreliable results Start Press button 4 Figure 1 A diagrammatic representation of the sequence of analysis Analysis is continued until all spermatozoa in the first small square A1 are characterized Then you proceed to the next small square A2 following a well defined sequence When all spermatozoa within the large square are analyzed press button 4 of the cursor If at this point less than 60 spermatozoa are characterized the preparation is shifted to another visual field to continue the analysis of another large square The analysis is completed when a sufficient number of large squares has been evaluated to characterize at least 60 spermatozoa This is indicated by a computer generated triple beep It is possible to interrupt the procedure before 60 spermatozoa have been characterized First complete counting the cells in least 1 large square press button 4 to indicate end of square and next click the Stop button in the dialog box on the screen You can cancel the analysis by pressing the Esc key or clicking the Cancel button The procedure described above yields optimal results in semen samples with sperm concentration between 5 and 50 x10 mL If the number of spermatozoa per visual field is less than 5 corresponding to severe oligozoospermia you can to classify each sperm prese
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