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mLIMS 2014 User Manual

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1. 130 2 In Review Animals List select List of Animals Displaying 1 15 of 15 records Page 1 of 1 dam and sire by checking the ehack boxes y rAddto Selection New Update J Delete J Show Deleted Records g Export f amp JEvent 3 sacritice Move 3 Click on Mating button NO Animal Name 5ex DOB Cage Background Strain Genes amp Genotypes Status Age Cage Purpose Cage Type Coat Ear Tag B6 1 F 2013 Cage Live Od Holding 09 1 26 2013 Cage Live d Holding 09 1 26 2013 Cage E Holding 09 1 26 neurogenin 1 M 2013 777333 Black 6 Ngn 1 PTEN Live d Holding Agouti 1 09 4 p 25 131 4 Set Up Mating window will Neurogenin 1 in brain tumor display Cages DEATH E DUE Selected Cages Animals Animals s Measurements Please select the animals to be included in this mating Lab Events i aa iat Ho Capes Animals Locations Matings Set Up Mating u FERE neuragenin 1g Building BSRB Room 56 8000 Review Matings Selected Matings 0 Cage 1 B amp 19 O B amp 29 Cl B639 Building BSRB Room SB 8000 t Search Matings oe DEM Main Information Projects Project Neurogenin 1 in brain tumor I Genes and Genotypes ee S 4 J Choose from all active cages IACUC Protocols Set up a new cage Billing Accomis hte tapes System Settings Mating Detail eee ing Details z Dam 2 Q A 5 Select sire and dam Main Information Note that other animals in
2. Date of Birth 2013 09 25 Coat we Strain w Background Strain v SOUrCE w Purpose v Genes and Genotypes Add More Click here to attach files 47 Ear Tag Add Multiple Animals 1 In left hand menu select Animals Add Multiple EH Main Information t Cages dps Main Information Animals Add New Animal Project Neurogenin 1 in brain tumor k Add Multiple Animals Automatically Create Cages Uncheck this option if you prefer to assign the animals to your own cages Review Animals Mo of Animals Review By Owner Animal Name Initial Review By Status Naming Rule All animals have the same name For example if you enter ABC in the Animal Mai Review By Age Recently Added Animals 3 Animal Information Sexi Female Date of Birth 2013 09 26 Selected Animals 0 Search Animals Measurements Coat Strain Background Strain Source 48 2 Select Project you can select E E E E Neurogenin 1 in brain tumor Main Information Projects selection list or main Cages nusec ooacadenoanaocoooaacodooooacoooo coaanoeooonaasocasaacacanoocancooonoh Jadabpansooscapdgaenoacadsooacacnaaeno aono oosonescescad Main Infor f io information window Project Animals field Add New Animal Project Neurogenin 1 in brain tumor I Add Multiple Animals Automatically Create Cages Uncheck this option if you prefer t Review Animals Ho of An
3. Live Add More If you have more than one hha criteria click on Add More Any Fields Browse Mif Browse Deleted gt Settings Hide Animal Name Background Strain Listof Cage of 97 records Page 1 of 1 Cage Purpose Cage Type WAGE Cause of Death date J Delete J Show Deleted Records z lExport Coat E Comments of Sacrifice tus Sex DOB Coat Ear Punch E date of tam ii Diet P M 2013 06 27 62 FP 3 ze B M 2013 06 27 783 E 1 EarPunch M 2013 06 27 782 E EAN F 2013 06 27 30 Pra v Genes m 3 Genotypes i F 2013 06 27 31 Pra x Litter 5 O Mating Parents M 2013 08 27 r E Motes M 2013 09 01 i2 A zi Origin 8 Owner P F 2013 04 16 Black 152 BRC i ee m Purpose P F 2013 04 16 yelow 123 BRE F s i M 2013 08 12 Black Der ource lal Status F 2013 08 12 Agouti 123 Der F Strain wb F 2013 07 24 Black 2 Der Search Status Is Live And Or Genes B Has PTEN l Remove Add More 4 Click on Search qo New Search wd Browse bal Browse Deleted y Settings es Hide 101 102 5 Search results will display Search Status Is Live x And Or Genes v Has a PTEN Add More q Mew Search ey Browse rf Browse Deleted Settings 3 H List of Animals Displaying 1 7 of 7 records Page 1 of 1 x rAddto Selection New Update J Delete J Show Deleted Records Exp No Animal Name 7 Status Sex Genes amp Genotypes DOB female123 Live
4. n 09 i i in brain E TE E SS NNI 26 tumor IACUC Protocols Billing Accounts P ee E d Cage d agg A list of animals created in the last 24 hours will be displayed 60 Review Animals 1 In left hand menu select f Neurogenin 1 in brain tumor Animals gt Review Animals Cages r Animals 9 Add New Animal 9 Add Multiple Animals Review By Owner hk Review By Status k Review By Age i Recently Added Animals 10 Selected Animals 0 Search Animals Measurements Matings Litters Projects Genes and Genotypes IACUC Protocols 29 Search pj Browse rd Browse Deleted Settings Print List of Animals Displaying 1 10 of 10 records Page 1 of 1 T Add to Selection No QD Gm Eo Un ak W he e p e Select an action 61 Animal Name neurogenin 1 neurogenin 2 neurogenin 3 neurogenin 4 neurogenin 5 neurogenin 6 neurogenin 7 neurogenin 8 neurogenin 9 neurogenin 10 gs Status Sex Live Live Live Live Live Live Live Live Live Live Zo ee TIE X X DOB 2013 10 09 2013 10 09 2013 10 09 2013 10 09 2013 10 09 2013 10 09 2013 10 09 2013 10 09 2013 10 09 2013 10 09 Coat Ear Punch Displaying 1 10 of 10 records Page 1 of 1 New 3 Update Delete J Show Deleted Records Export Cage Cage 1 Cage 1 Cage 1 Cage 1 Cage i Cage 2 Cage 2 Cage 2 Cage 2 Cage 2 Event 3 sacrifice Cage Purpose Neurogeni
5. Live Live Live Live Live Live Live M 73 Genes amp Genotypes FGFS null FGFS FGFS het FGFS het FGFS FGFS het FGFS het FGFS t Neurogenin 1 Neurogenin 1 het Neurogenin 1 Neurogenin 1 Neurogenin 1 wt Neurogenin 1 Neurogenin 1 Neurogenin 1 DOB Coat Ear P 2013 10 09 2013 10 09 2013 10 a Q 2013 10 09 2013 10 gae 2013 10 09 2013 10 08 2013 10 List of Deleted Animals Displaying 1 2 of 2 records Page 1 of 1 ONew E Export Show Active Recor Undelete Animal Name Status Sex Genes amp Genotypes neurogenin 1 Live M FGFS Neurogenin 1 het neurogenin 2 Live M FGFS Neurogenin 1 null Select an action A Displaying 1 2 of 2 records Page 1 of 1 74 DOB 2013 10 09 2013 10 09 Sacrifice Animals 1 In Review Animals List select the animals that you want to sacrifice by checking the check boxes 2 Click on Sacrifice S F Add to Selection Animal Name neuragenin 3 neurogenin 4 neurogenin 5 neurogenin 6 neurogenin 7 neurogenin amp neurogenin 9 New List of Animals Displaying 1 8 of 8 records Page 1 of 1 Update T Delete T Show Deleted Record Status Sex Live Live Live Live Live Live Live 75 M Genes amp Genotypes FGFS null FGFS FGFS het FGFS het FGFS
6. Method 3 Neuragenin 1 in brain tumor Cages In Review Cages List select s Create Cage the cages for the event and Review E click on Event bbs rein i Breeding 4 al Holding 8 Ww Discarded Review By Owner Recently Added Cages 13 Selected Cages 0 amp Search Cages i Cage Map RE P eee ree Lab Events SUID n ES s A n ur TRUM MUR NUN NN TUN asin ane amri 47 nanan In New Event window the selected cages and animals display Select the animals for this event by checking unchecking the check boxes 29 Search Browse g Settings Print List of Cages Displaying 1 12 of 12 records Page 1 of 1 F Add to Selection No cage Name p i Fy 2 2 4 IF 5 Fl 6 p Fi 8 207 3000001 65652223 7000000 9000000 AZ Cage 5 Cage 6 Cage 7 Status Active Active Active Active Active Active Active Active Purpose Setup Date Halding Holding Breeding Breeding Holding Breeding Holding Holding New Update F Discard Cages 2013 10 13 2013 10 13 2013 10 12 2013 09 05 2013 10 13 2013 10 12 2013 10 12 2013 10 13 k ub 87s m Projec Cage Cards Animals Ov Na Iw Ma Iw Male Female 1 Female 2 Ma Female 3 Iw neuragenin 1 B6 1 neu d neu Na g neu neu neu neu neu neu Iw neu neu neu 38 neu 39 ne
7. feld 9 Add Multiple Animals Neurogenin 1 in brain tumor z Review Animals Review By Owner Review By Status Review By Age Recently Added Animals 2 3 Set up a cage for the animal Main Information Project Neurogenin 1 in brain tumor Choose from all active cages Set up a new cage Main Information Main Information Project Cage Neurogenin 1 in brain tumor Choose from all active cages O Set up a new cage N A e g Animal is not available or not alive Owner Maomi Iwai About This Animal N A e g Animal is not available or not alive You have three options Choose from an existing cage Choose from all active cages the drop down menu displays all active cages in the Project Cage name Cage Purpose Holding or Breeding and number of Males and Females in the cage are displayed Set up a new cage ni Kk a 1 Owner Naomi Iwai i N A e g Animal is not avai la 77333 Holding M 1 F 0 7 7334 Holding M 0 F 1 777335 Breeding M 0 F 0 Set up a new Cage Main Information Project Neurogenin 1 in brain tumor When you click on this option a Create Cage window displays Enter all mandatory information About This Cage and click on Save Note that Cage Name some values such as IACUC Protocol and cage Locations have already been prepopulated based on your Project default settings You can chang
8. l E Search wil Browse ra Browse Deleted 4 Settings Print Cages Animals 9 Add New Animal m A o T Talant a LT 9 Add Multiple Animals T Add to Selection New Update J Delete J Show Deleted Records Review By Owner al 1 2 Live 2013 06 27 Review By Status 13 Live 2013 06 27 Review By Age Recently Added Animals 10 Selected Animals 0 14 Live 2013 06 27 30 Live 2013 06 27 Jl Live 2013 06 27 Search Animals V ea NORRIS ee EET 134 Live 2013 06 27 P eee A i Live 2013 09 01 Lab Events Pad ar e IDEE SERERE QURE ERE UNE D REED TE RD A TE GR DT DU BRCA female 1 live 2013 04 18 Black 3 3 4 5 o Fi 8 z Matings RE DA EU EEG ERU or Ur Wr ESE EEE SAE ge ge rr T We WI Eur EXE Ur E RU UP KU rar BRCA female 2 Live 2013 04 16 yellow Litters i EA Demo 4 1 Live 2013 08 12 Black E pue Projects Il Il Demo A 2 Live 2013 08 12 Agouti 63 My Animals In top menu bar you will find a shortcut button called My Animals If you click on this all your animals will be displayed in one list HOME Hello Naomi Iwai LOGOUT Admin My Calendar My Animals My Cages Environment Test showing All Projects Search pd Browse rd Browse Deleted j Settings Print Cages vicc en etait rata oy List of Animals Displaying 1 92 of 92 records Page 1 of 1 Animals i ide Add New Animal y selecti OWew yu E 3 Shov ed R O Add Multiple Animals T Add to Selection New Update J Delete J Show Delet
9. t Users Help 27 2 Enter all mandatory information and click Save Main Information ACUC Protocol Title Note Protocol Abbreviation is an abbreviation that you choose to distinguish a protocol as protocol Protocol Number titles can be very long Throughout the system protocols will be displayed as protocol abbreviation protocol number Expiration Date Protocol Abbreviation Approval Date 3973 09 74 Total Animals Quota Notes For more information about IACUC Protocols refer to Section 9 IACUC Protocols Click here to attach files Section 3Setting Up Your Personal Preferences 28 Personal Preferences You can decide how you want to open a window or tab 1 In left hand menu select System Showing All Projects Main Information Settings Personal Preferences System Settings m Settings m Users Permissions Personal Preferences 2 How do you like to open the new page How do you like to open the new pagel came Window Pop Up New Window Same Window 29 If you choose Pop Up each time you click on a record a pop up window will display see screenshot at right List of Animals Displaying 1 50 of 96 records Page 1 of 2 v rAddto Selection New Update J Delete J Show Deleted Records g Export If you choose New Window the NO Animal Name Sex DOB Cage Project Background Strain Genes information will display as a new i 12 M 20
10. 112 Shortcut buttons for Review Cages List List of Cages Displaying 1 13 of 13 records Page 1 of 1 Record per pag y rAddto Selection New Update J Delete export Event CageCards Project J Discard Cages CageReport Mating Add to Selection see Section 5 Animals gt The Animal Shopping Cart New Create Cage button see above Create Cage Update Update Cage Information button Delete Delete Cage button see Section 5 Animals gt Delete Animals for more information Export use this button to export your records to Excel Event see Section 8 Lab Events Cage Cards see below Cage Cards Project Move Cages between Projects see below Move Cage to Different Project Discard Cages see below Discard Cages Cage Report see below Cage Report Mating see Section 7 Matings and Litters Note you can change the order of these buttons by dragging them left and right 113 Discard Cages When you are done with a cage discard the cage the status of a cage can be either Active or Discarded Do not delete a cage unless it is an erroneous record 1 In Review Cages List select arogenin 1 in brain tt J Search iy Browse Settings Print the cages that you want to Meurogenin 1 in brain tumor I 3 Wi 7 g g Cages discard by checking the O create Cage List of Cages Displaying 1 13 of 13 records Page 1 of 1 check boxes i Review Cages 2 Click on Discard Cages Active 3 FrAddto Selec
11. 180 You will see the cages you just Cages Just Createth created 5000001 6662723 7000000 37303688 76690393 Cage 1 Cage 2 Cage 4 Cage 5 Cage 6 Cage 7 Cage B cage B cage 73 cage 33 cage a Cage A1 cage d male Neurogenin 56 9000000 H 181 For each pup select the weaning cage from the cages you just Sex created Set the first 3 animals to f Update Create Cage Click Save and pups will be The rest of the animals will be set to opposite Auto Create Cages assigned to the selected cages F Iv Agouti 5000001 i F Agouti 5000001 F Agouti 5000001 M Agouti Agouti 182 Option 3 Choose from existing Cages Just Created cages in drop down list and 3000001 distribute females and males For each pup select a weaning cage from the list of existing cages Other Cages in drop down list 5000001 6662223 7000000 37383888 76690393 Cage 1 Click Save and pups will be Cage assigned to the selected cages Cage 3 Cage 4 Cage 5 Cage 5 Cage 6 Cage 7 Cage B cage B cage 33 cage a Cage A12 cage d fem1 9000000 Review Litter by Status In left hand menu Litters you can review your litters by Status Active Not Tagged Not Genotyped Not Weaned Weaned By default when you click on Review Litters you will see Active litters 183 lt n Measurements Litters 9 Add
12. 4 trind Live I 4 femme 190 Copy female 7 i female 7 female 7 B Sex Set the first 1 animals to Update The rest of the animals will be set to opposite E 1 191 Create Cage Auto Create 10000 10000 10000 Parents Cages 5662223 7000000 7693832 9000000 37383688 76690393 A rara L E In Litter information confirm Litter Edit Create amp Search that the pups are assigned to the correct breeding cage with the Be Cages I mme Breeding Cage Parents 3 Audi mother Cage Name Status Purpose Setup Date Animals female 7 triol ctive Breeding 2013 10 14 trio2 trio3 triof trio5 Note that when you open cage information for cage 70000 where female 7 and her pups reside there is a tab called Other Matings Cage 4 Edit Create X Search Cage Card Cage Report 4 Edit All An p EDEN summary Cage Map Animals 6 q Other Matings 1 J Litters 1 Animal History 1 Ho Mating Parents Status age Mating Date Plug Da trio mating male 13 female 7 female B Active 10000 2013 10 14 2013 10 This tab indicates that animals in this cage are part of a mating although it is not the original breeding cage 10000 193 Complete Or Cancel Mating When you are done with a mating change the status to Completed or Canceled Do not Delete a mating
13. FGFS het FGFS hat Neurogenin 1 Neurogenin 1 het Neurogenin 1 Neurogenin 1 Neurogenin 1 wt Meurogenin 1 Neurogenin 1 DOB Coat E 2013 10 ga 2013 10 gg 2013 10 gg 2013 10 gg 2013 10 gag 2013 10 ga 2013 10 na 3 The Sacrifice Animal window Nn E N displays 4 Enter Date of Death Animals To Be mandatory and other S a neurogenin 3 neurogenin 4 neurogenin 5 2 Sacrificed 3 information if applicable i Main Information Note if you check Discard Cages Date of Death if it is empty the system will automatically discard the cage Cause of Death only if there are no other live animals left in that cage Discard Cages if it is empty Comments of 5acrfice 5 Click Save 76 6 Note that the sacrificed Cages animals are not visible poss anymore 9 Add New Animal 7 To view sacrificed animals Add Multiple Animals click on Animals Review By Review Animals Status kh Review By Owner Review Selected Animals 4 Ct Search Animals hk Saved Searches 8 Click on Not Alive List of P Cages 9 Add New Animal 9 add Multiple Animals Note that in mLIMS dead animals Review Animals are displayed with strikethrough k Review By Owner Review By Status a Live 5 Not Alive You can revive dead animals by clicking Update and changing the Status to Live t Review By Age Selected Animals
14. Neurogenin 1 Neurogenin 1 Neurogenin 1 he Meurogenin 1 Meurogenin 1 Neurogenin 1 v Neurogenin 1 Neurogenin 1 Message displays How do you like to update the selected records If you want to make different updates for each record choose the first option Each selected J record will have different values If you want to make the same update for all selected records choose the second option All selected records will have the same values Choose an option and click on Update How do you like to update the selected records Fach selected record will have different values O All selected records will have the same values What s the difference Cancel 91 Each selected record will have different values option No You can make different updates for each animal and field Animal Name neuragenin 1 neurogenin 2 neuragenin 3 neuragenin 4 neuragenin 5 Genotypes j amp FGFS tit X Neurogenin 1 het Add More X FGF8 x im X Neurogenin 1 null Add More X FGF8 im Pd Neuragenin 1 null Add More 4 v X FGFS IT 4 iv X Neurogenin 1 het Add More X FGFS m 92 DOB 2013 10 09 2013 10 09 2013 10 09 2013 10 09 2013 10 09 All selected records will have the same values option Check the check box for the field you want to up
15. ONLINE MOUSE INFO SYSTEM mLIMS 2014 User Manual Last Update April 2014 Contents Section 1 Getting Started With mLIMS EGG MICO MGM Poca duct TENTER EET 5 Bias lea eiRi uim i i B TT 6 Section 2 Setting Up Your mLIMS System Manadge USerBebrmisslOlSeaeteodtroestcsdatosienturbehese to iund rode ostwadu O nartestunacisotuberacoiun ie doe rocd end odd abet utendi eor E 7 SEEM Settings FOr VO Wie Jabsseeatiodvis eso woe dol rV ak re orn A poids God ee area oie wa det dILIV I DIVIMEM AE OAT O 10 Set Up Genes and GenOcy eS cai TT TT D E I MEE 23 ETIedgte TACUC PFOLOGOR usi ves ordei E dado ud dc iMm AM MD Cee rT eC ret ne ee ree ee eT rear eee 27 Section 3 Setting Up Your Personal Preferences Personal PrelerentcES EET E ETE P 29 Section 4 Setting Up Your Projects Cregu e PIOIGGEL dotum MODUM DIIEI d Deu id Edu Dt id id EIE DIM DP EIE De DII nda beuinotaranseadatemine temas EMI DEI DEI PER MEME 32 Section 5 Animals Aag ew ANIMA ss sed a c ee cet ose ita i edlen Sa ipia a seas ella a aaah aes LI REEL ME pM Recent ELLA Cv POL a PIER LM E 43 A adulte JAPRIETISIS sc sdatuuttdss uaa E tema E et Rad aas Not ayer Ru adu tM Dg E MM EAT E PLA sU RCau Pas ced eed UEM UT RUE 48 pe Ie AMI Milas na aa orm metti eb a COD e cM D eet CEOs Roun D Mod Cas DIM DNUS E TUDrDP ELM DLE DUDDE IDEE 61 TwecAnimast
16. 4 C Search Animals 7 List of Animals Displaying 1 5 of 5 records Page 1 of 1 3 rAdd to Selection ONew Update J Delete T Show Deleted Record l No Animal Name Status Sex Genes amp Genotypes 1 neurogenin amp Live F FGFS Neurogenin 1 het 2 neurogenin 7 Live F FGFS Neurogenin 1 wt neurogenin 8 Live F FGFS Neurogenin 1 het neurogenin 9 Live M FGFS Meurogenin 1 het Al Al Al m List of Animals Displaying 1 3 of 3 records Page 1 of 1 yTAddto Selection New Update J Delete Sacrifice Mating I No Animal Name Status Sex Genes amp Genotypes TRAIL Sacrificed M FGF8 Neurogenin 1 null E E TeLIeR Sacrificed M FGFS Neurogenin 1 he E Sacrificed F FGFS Neurogenin 1 het Select an action Displaying 1 3 of 3 records Page 1 of 1 Move Animals You can move animals to a different cage within the same Project or to a different Project 1 In Review Animals List select List of Animals Displaying 1 5 of 5 records Page 1 of 1 the animals that you want to MOVA DY En SCIT ene creer y rAddto Selection New Update J Delete J Show Deleted Records 9 sac boxes 2 Click on Move 4 F YO Animal Name Status Sex DOB Cage Cage Purpose Genes amp Genotypes af neurogenin 6 Live F 2013 10 Cage Holding FGFS Neurogenin 1 09 2 het neurogenin 7 Live 2013 10 Cage Holding FGFS Neurogenin 1 09 2 neurogenin
17. 5 of 5 records Page 1 of 1 VrAddto Selection New 3 Update Cj Delete E Export No 1 Litter neurogenin 1 neurogenin 2 neurogenin 3 neuragenin d neurogenin 6 Mating neurogenin neuragenin 1 neurogenin 1 neurogenin 1 neurogenin 1 Date of Birth Tagging Date Gen 2013 10 11 2013 10 13 2013 10 13 2013 10 13 2013 10 13 2013 10 23 2013 10 23 2013 10 23 2013 10 23 Genes and Genotypes erro MER RU 3 E E E T Select an actian Displaying 1 E 5 of 5 records Page 1 of 1 IACUC Protocols Billing Accounts Guctem Settinas 168 3 Make any update in the Litter Information window 4 For each update you make make sure that the Litter Status is updated in the Progress box for more details see below Tagging Main Information Mating neurcgenin 1 Breeding Cage 000000 About This Litter Litter neurogenin 6 Email Notifications l Hates Additional Infarmation for Pups Strain Backgrecurnd Strain Category Gener ation Existing Pups Hn Animal Mame Actions Ve ee eel ee ae Ee 7 Fl UP a a CEDE ai Teorey E Pin P AE DE LEBESCI nm F a a 169 Toe Clip A uto Fill Progress Is Active Date of Birth ls Tagged Is Genoty ped a E Weaned io Weaning Date rex Set the first 1 animals ta Update tof the animals will be set to oppo SL 2013 10 13 2013 10 23 2013 11 03 Cage Cr
18. IACUC Prot 2 Click on Cage Report vy 1 Cage 5 Active Holding 2013 10 neuro 1 neuro 2 neuro Naomi brain tumor 12 3 neuro 4 neuro 5 Iwal 11 2346 Cage 6 Active Holding 2013 10 neuro 6 neuro 7 neuro Naomi X brain tumor 12 8 neuro 9 Iwal 11 2346 Cage A12 Active Holding 2013 09 male 107 male 13 Naomi brain tumor 14 male 14 male 15 male Iwai 11 2346 16 cage d Active Holding 2013 09 CDFG9 Naomi brain tumor 14 Iwal 11 2346 Meurogenin Active Holding 2013 09 Naomi brain tumor 56 14 Iwal 11 2346 cage a Active Breeding 2013 10 CDF 10 male 7 Naomi brain tumor 121 3 Cage Report displays Cage Cage A12 Setup Date 2013 09 14 MDA Cages 76555555 Locations BSRB SB 8000 1 Owner Naomi Iwai PI RISTS Project Neurogenin 1 in brain tumor IACUC Protocol brain tumor 99 11 2546 Billing Account 11 22222 No An irl Sex DOB Coat Ear Punch Strain Genotypes 1 male 107 Maie 2013 09 03 Black 111 FGFS Meurcgenmn 1 null 2 male 13 Mals 2013 10 11 FGFS 3 male i4 Mals 2019 10 11 Agouti FGFB 4 male 15 Maie 2013 10 11 Agouti E FGFS Cage Cage 2 Setup Date 2013 10 12 Owner Naomi Iwai MDA Cages 2009332 PI RISTS Locations BSRB 5B 8000 Project Neurogenin 1 in brain tumor IACUC Protocol brain tumor 99 11 2346 Billing Account 11 22222 No Ani nal Sex DOB Cost Ear Funch Strain Genotypes neuro i Female 2013 10 12 Aqpouti BOS Neurogenin 1 het Z meur
19. a The animals have been moved to the target cage Cage 7000000 150 13 Click on Animals tab and Matings tab to confirm that the added females now reside in the breeding cage and are part of the mating Cage E Edit 9 create Search Cage Card Cage Report 4 Edit All Summary Cage Map Animals 4 Matings 1 Animal History 6 Audit Trails 1 Main Information Project Neurogenin 1 in brain tumor IACUC Protocol brain tumor 99 11 2346 Billing Account 11 2222 Owner Naomi Ihwal About This Cage Cage Name 7000000 MDA Cage Purpose Breeding Type Locations Building BSRB Room 56 8000 Status Active Setup Date 7013 10 12 Date Discarded Hotes 151 summary Cage Map Animals 4 Matings 1 Animal History 6 Audit Trails 1 Ho Animal Name Status Sex DOB Owner Cage Cage Purpose 2013 08 Naomi 1 Male lve M QUU 7000000 Breeding 15 Iwai 2013 08 H TAI ll 2 Female 1 live F aami 7000000 Breeding b 20 lwai 2013 08 Naomi 3 Female 2 live F i 7000000 Breeding t 1 lwai 2013 09 H TII 1 4 Female 3 lve F i 7000000 Breeding b 03 Iwai summary Cage Map Animals 4 Matings 1 Animal History 6 Audit Trails 1 Ho Mating Parents Status Cage Mating Date 1 Test Mating 1 Male Female 1 Female 7 Female 3 Active F O0Q000 2013 10 12 Note To set up a mating with more than one dam make sure that the
20. unless it is an erroneous record When you want to discontinue a mating change the Status to Mating trio mating Completed or Canceled Q Dam Mating Details A V female 7 J female 8 Cj Sire C V male 13 Mating Date 2013 10 14 Plug Date 2013 10 15 Is Pregnant py Preenancy Date Delivery Date 2013 11 03 Retre Date Status Active Active Completed Email Notifications Notes Canceled 194 In Matings gt Review Matings By Status you can Neurogenin 1 in brain tumor 29 Search aj Browse g review Active Canceled Cages and Completed Matings RACE List of Matings Displayiri separately Measurements 3 Y zrAdd to Selection Ne Lab Events Matings No Mating Set Up Mating m 1 neurogenin 5 Test Mating 1 Review Matings Review Mating By Status l Active 5 F neurogenin 3 WE Canceled 1 neurogenin lj Completed 1 2 Selected Matings 0 E neurogenin A Mating amp Litter Email Notifications mLIMS will send email reminders for Mating and Litter related events Mating emails Mating reminder Delivery reminder Retire reminder Litter emails 195 DOB reminder Tagging reminder Genotyping reminder Weaning reminder The emails will be sent on the day of the scheduled event at 12 00am Mating Delivery Retire Reminders When you create a mating you can set up Mating Date Delivery Mating Details Date and R
21. 0 Selected Cages Animals matme emails 26 male fem 3 Litter DOB Tagging Genotyping Weaning Reminders 197 When you create a litter you can set up Date of Birth Tagging Date Genotyping Date and Weaning Date and you can select the email recipients If DOB is today or a past date no email reminder is generated see below Mating and Litter email generation rules Main Information Mating neurogenin 1 Breeding Cage 9000000 About This Litter et 1 Litter neurogenin 4 Email Notifications y Notes 198 ls Active 2013 10 13 ls Tagged Tagging Date 2013 10 23 ls Genotyped Genotyping Date ls Weaned Weaning Date 2013 11 03 Email Notifications Naomi Iwai David Cotten Debra Montague Jane Doe Nami Hei Naomi Test HMatalia Chane Nina Chana On the day of the scheduled DOB Tagging Genotyping or Weaning at 12am selected recipients will receive an email to their MDA email address The email subject line will indicate the type of reminder DOB Tagging Genotyping or Weaning and the Litter Name and date of scheduled event The email content will display all information related to this litter including links that direct you to the litter in mLIMS mLIMS lt do not eply mlims amp mdanderson org gt To Iwai Naomi Cc Subject More Details Litter Info Litter Date of Birth Tagged Date Genotvped Date Weaning Date Is Active Is Tagzed Is
22. 14 Live gt 9 neu 102 F 2013 10 13 Live g neu44 M 2013 10 13 Live Offspring This animal has no offspring In this example you are reviewing the parents of animal neu 111 Parents Open Al Close AL 4 c neu 111 M 2013 10 14 Live neu 102 F 2013 10 13 Live g neu 44 M 2013 10 13 Live amm d a Click on the arrowheads next to the parents or click on Open All to display more generations 86 Parents 4 d neu 111 M 2013 10 14 Live 4 9 neu 102 F 2013 10 13 Live g neurogenin 1 M 2013 08 01 Live be 9 B6 1 F 2013 08 14 Live 4 g neu 44 M 2013 10 13 Live d neurogenin 1 M 2013 08 01 Live 9 B6 1 F 2013 08 14 Live Offspring 87 Update Animal Information There are two options for bulk updating animal information see below in Update Multiple Animals Update One Animal Cages In Review Animals List select the Animals i 9 Add New Animal animal that you want to update d by checking the check box Add Multiple Animals List of Animals Displaying 1 10 of 10 records Page 1 of 1 rAdd to Selection New E uptate yi Delete j Show Deleted Records AE EE Review Animals No Animal Name Status Sex Genes amp Genotypes DO Review By Owner 1 neurogenin 1 Live M FGFS Neurogenin 1 2013 het 09 f 2 neurogenin 2 Live M FGES Neurogenin 1 2013 Note you can also click on the Review By Age null Qo animal name which takes you to Recently Added Animals 10 Im 3
23. 4 cMyb PTEN 2013 04 12 Demo RISTS 2 Live cMyb PTEN 2013 0 01 Demo RISTS 1 Live 4 cMyb p27 PTEN 2013 0 H 13 Demo A 2 Live PTEN 2013 0 12 Demo A 1 lve cMvh PTEN 2N13 ni 103 6 To save this Search click on th Status i Ive Smart Search Search Status Live This is for searches that you perform frequently Add Mare 6S New Search hi Browse B Browse Deleted j Settings Hide MF Pri List of Animals Displaying 1 7 of 7 records Page 1 of 1 And Or Genes PTEN l Remove L Smart Search YFAdd to Selection New H Update Jj Delete J Show Deleted Records z Export Event sacrifice Move Animal Name 7 Status Sex Genes amp Genotypes DOB female123 Live M cMyb PTEN 2013 08 12 Demo RISTS 2 Live F cMyb PTEN 2013 08 0i Demo RISTS 1 Live cMyb p27 PTEN 2013 08 13 emo 2 Live l PTEN 2013 08 104 Coat Ear Punch Black Li Black 123 Black Agouti 123 Cage Demo 6 Demo RISTS 5 Demo RISTS 5 Demo 3 Smart Search Manager window displays Enter a name for the Search Click Save Smart Search Manager Save This Search Name PTEN animals If you want to save as a new search please type a different name Include the result in the summary 105 POST Ee UE WISI yop want showing All Projects AJ Search pu Browse f Browse Delete
24. 7 neuro 8 7000000 neuro 9 P Review Events By Status F cdf New CDF 7 CDF 8 CDF 9 2013 10 13 z Selected Events 0 H Review Events By Researcher tt Search Events Ea Calendar View Select an action Displaying 1 2 of 2 records Page 1 of 1 List of Animals Displaying 1 4 of 4 records Page 1 of 1 new Update 9 Sacrifice Mating Move Event J Delete Export Y Remove from Selection Keep In Mo Animal Name Status Sex DOB Cage Cage Purposg Event Summary enes amp Genoty F 1 Female 1 Live F 2013 7000000 Breeding BrdU injection New 2013 10 30 Meurogenin 1 h B 20 Female 2 Live 2013 7000000 Breeding BrdU injection New 2013 10 30 Neurogenin 1 w 08 21 Female 3 Live F 2013 7000000 Breeding BrdU injection New 2013 10 30 Meurogenin 1 w 09 03 Male Live 2013 7000000 Breeding Brdu injection New 2013 10 30 nid 211 Method 3 In Review Cages List under Event Summary the Event Title Event Status and Event Start Date End Date are displayed for each cage Method 4 Click on Calendar View under Lab Events Or Click on My Calendar in top menu bar List of Cages Displaying 1 12 of 12 records Page 1 of 1 SFAdd to Selection New Update J Discard Cages 2Project Event Cage Cards Cage Name Status Purpose Setup Date Animals Active Holding 2013 10 neuro 7 1 neuro amp neuro 9 Cage Breeding 2013 10 Male 12 Female 1 Female 2 Fe
25. 9 0 qe s Wy Anicia Roberson 27 gt rr est project 09 05 m5 33 F 2013 PracB Practice B P Litters 27 Projects Fl 6 134 M 2013 783455 xyzA If you have long Project names the Projects selection menu will become wide leaving less space on the right for the main work area 34 6 Your Project has been created Project Edit 9 create amp Search 7 Click on Edit button Summary Main Information Project Meurogenin 1 in brain tumor Record Naming mU Animal Disable Auto Maming Cage Disable Auto Naming Mating Disable Auto Naming Litter Disable Auto Naming Protocol brain tumor 99 888 777 Billing Account Owner Naomi bai Notes 35 8 Select Record Naming tab and set up your preferred record naming rules You can decide how you want to name your records in this Project Disable Auto Naming system will not auto name your record name field will be blank and you will type the name Based on Abbreviation enter your desired abbreviation The system will use this abbreviation plus a system generated number to create a name Tip keep your abbreviations short For Animals only Animal Tag system will name your animals with the animal tag number e g ear punch toe clip Abbreviation Animal Tag system will name your animals with your abbreviation plus animal tag number Main Information Animal Record Naming Cage Locations Mating amp Disable Auto Naming Animal
26. Ahin By Caena My Anmah Ay Cages Environment Test there are two areas of Showing Al Projects MI navigation the left Cages IN MI S li Colony Overview Section hand menu and the Animat e CINTIME AOLE IM PLU Penne ngs colony overview Lah Events nges 9 Pu Matings Active ae Lire Lg Active Litters Breeding Missing Protucols e g Active and Pregnant Prejacts Holley a o Live Animals without Cange Empty 214 Gene as Coemofypes Chuercreredied gt i 21d 6m IACLIC Proteculs Missing Protucols 3 m iy Billing Accounts Iy Triba Sablings Animals section ind Users E i Litters Lab Events i ACUC Protocols Halp Active d Active 1 TAGS Prodncols Not Tagged 1 Axsigned To Me J Exceed sania Not Genotypes z od Aa pipri Expires w h Live fundens Left Hand Menu Not Weaned 42 Hew Propet Ta Progress Section 2 Setting Up Your mLIMS System This section explains how to set up system settings for the whole lab This setup should be performed by a designated person in the lab who we will call lab mLIMS manager in this manual The lab mLIMS manager should be the only user in the lab who has full access in the system besides the PI Manage User Permissions 1 In left hand menu select System Settings gt Users Permissions You will see a table with the names of the users in your lab and various permission levels 7 T HOME Help Na
27. Animals records 14 Live 30 Live Saved Searches Measurements 31 Live Lab Events r1 Live 69 5 Click on Selected Animals Cages Be eae eae ee eae a 6 Your selected animals will be Animals Displaying g 9 Add New Animal 9 add Multiple Animals From here you can use these peyiew Animals No animals to set up matings sacrifice them set up an event etc displayed in a list z Remove from Selection 5FKeepin Selection Mating Sacrifice Event Animal Name Status Sex Genes amp Genotypes DOB Review By Owner 1 12 Live M 2013 06 2 Review By Status 30 Live F 2013 06 2 2 Review By Age 3 DemoRISTS 8 Live M 2013 08 1 Selected Animals 4 n 4 4 E 2 Live F 2013 09 1 Search Animals Select an action Displaying 1 4 of 4 records Page 1 of 1 To empty the Shopping Cart use Cages E M pd NCC M CN T button 9 Add New Animal 9 add Multiple Animals List of Animals Displaying 1 4 of 4 records Page 1 of 1 Keepin Selection Mating 9 Sacrifice Event Review Animals No Animal Name Status Sex Genes amp Genotypes DOB hk Review By Owner 1 12 Live 2013 06 2 Review By Status 30 Live 2013 06 2 Review By Age Demo RISTS 8 Live 2013 08 1 Selected Animals 4 E 2 Live 2013 09 1 Select an action Displaying 1 4 of 4 records Page 1 of 1 70 Delete Animals You can delete animals and recover them at any time This Delete function is a
28. E continued Level Location Type Set up as in screenshot to the right choose Row and Column Row Row Column Column 12 6 Cage Cards tab Main Information Capes Cage Cards Select 3 x 5 For Laser Printer 2 x95 Far Laser Printer 3x 5 For Laser Printer 3 xT For Zebra Printer 7 Animals tab Main Information Main Information Cages Cage Cards Animals Matings Litters Protocols Projects Users Uses Multiple Species Yes No Main Information If your lab uses only mice select No Uses Multiple 5pecies Wo i No of Dam and No of Sire Adult Definition 2 The number of minimum days to be considered as an adult Refers to the number of parents per animal e g if you enter 1 for Dam Mo of Dam 4 Maximum number of dam per animal up to 5 each animal can have only one mother If you frequently use trio matings and get pups for which you Age Format Year Month Day 1y2m27d do not know who the mom is you can set the value to 2 and the system will let you record two females as the ls Genotype Case Sensitive No e g If itis sensitive HET and het are considered two different genotypes mom for the pups Mo of Sire 4 Maximum number of sire per animal up to 5 Allow Duplicated Animal Mame Yes Allow Duplicated Animal Name Yes No If you choose Yes users can assign the same animal name to more than one animal If you choose No the system will give a warning when a
29. E i neurogenin 1 Live 2013 10 09 Cage Review By Status 2 neurogenin 2 Live 2013 10 09 Cage Review By Age E neurogenin 3 Live Recently Added Animals 10 2013 10 09 Cage neurogenin 4 Live 2013 10 09 Cage Selected Animals 0 neurogenin 5 Live 2013 10 09 Cage gt Search Animals Cel ore erence neurogenin 6 Live 2013 10 09 Cage MO MEE LIS neurogenin 7 Live 2013 10 09 Cage Lab Events neurogenin 8 Live 2013 10 09 Cage a eee Oe ee a d DU X Matings m TEE LI 94 2 Select the fields that you want to see as columns in the list Use the arrowheads to move fields back and forth between the Available Fields list and the Selected Fields list 3 In Selected Fields list arrange the order of display Use the arrows to move fields up and down when you move a field up the column will move to the left in the List of Animals Available Fields Selected Fields Animal Name Status Am Sex IDOB All Cause of Death an gt Coat Comments of Sacrifice Ear Punch Dam Cage date of tam Cage Purpose Diet Neurogenin 1 DOD FGF8 Select Fields to Display Age Age with DOB Background Strain Cage Type Select Fields to Display Available Fields Selected Fields Animal Name Status Age Age with DOB Background Strain Sex Cage Type DOB Cause of Death Coat gt gt Comments of Sacrifice Ear Punch Dam Cage date of tam Cage Purpose Diet All Meuro
30. Edit create x Search summary Pups 7 Cages 2 Mating Breeding Cage Animal Name Status Sex DOB Owner 2013 Haomi 10 13 wal Naomi lal Naomi haat 2013 Haomi Liv ui 10 13 bus 2013 Maomi 10 13 haat 2013 Naomi 10 13 Iwai 2013 Haomi 10 13 wai 176 Parents 2 Audit Trails 4 Cage Purpose Genes amp FGF amp Holding 1 het uii amp DET FGF8 N Holding 4 wil m WE FGF8 t 1 wt FGFS Holding fee Sem IIGI FGF8 f 1 het FGFS Holdin mS 1 het FGFS Holding 1 het n MWL Option 2 Create Cage Click on Create Cage Cage Auto Create Cages 9000000 I x Add More 9000000 X Add More 9000000 X Add More S000000 177 Create Cage window displays Enter information and Save Main Information Project Neurogenin Owner Naomi Iwai About This Cage 1 in brain tumor i Cage Name MDA Cage TACUC Protocol Billing Account Purpose brain tumor 99 11 2546 11 22222 Holding Type Setup Date 2013 10 13 Notes 178 Building BSRB Room 56 8000 Rack Row Column A message displays The new cage has been added to the cage drop down menu Click on OK Create all weaning cages you need 179 Click on arrowhead in cages drop down menu Create Cage Auto Create Cages 9000000 9000000
31. Female 2013 10 12 Black BOE Neurogenin 1 het je Cage 6 Setup Date 2013 10 12 Owner Naomi Iwai MDA Cage PI RISTS Locations BSRB 5B 8000 Project Neurogenin 1 in brain tumor IACUC Protocol brain tumor 99 11 2346 Billing Account 11 22222 No Animal Sex DOB Coat Ear Punch Strain Genotypes 1 meum amp Female 2013 10 17 Ati R11 Neurcgemmn 1 wt 122 Cage Map The Cage Map function allows you to view your cages in a rack view If you enter all cage location fields i e Building Room Rack Row and Column for each cage you can view your cages in Cage Map Note to use the Cage Map function all cage location options for Building Room Racks have to be set up in System Settings Settings Drop Down List tab by your lab mLIMS manager see Section 2 Setting Up Your mLIMS System Main Information Owner Naomi Iwai I About This Cage Cage Name cage 33 MDA Cages IACUC Protocol brain tumor 99 11 2346 Billing Account 44 2222 Purpose Holding Type Status Active setup Date 2013 10 11 Notes 123 Building Room Rack Row Column Locations BSRB SB 8000 1 In left hand menu select EE I Showing All Projects Cages gt Cage Map oleracea di kunt Cages 9 Create Cage Review Cages a Breeding 42 lal Holding 133 wl Discarded H Review By Owner Recently Added Cages 13 Selected Cages 0 w Cage Map Anim
32. Halding Holding Holding Holding Holding Holding Holding Holding Halding Holding het null null het het het wt 2 Select Project Neurogenin 1 in brain tumor J Search y Browse Browse Deleted gt Settings MT Prin If you select a Project you will 2 Cages see all animals in the Project UTE PODODOD Db List of Animals Displaying 1 10 of 10 records Page 1 of 1 Animals Add New Animal n o E l l L i um f 9 Add Multiple Animals F Add to Selection New Update J Delete J Show Deleted R E Review Animals l NO Animal Name Status Sex DOB Coat Ear Review By Owner el i neurogenin 1 Live M 2013 10 09 Review By Status rl 2 neurogenin 2 Live M 2013 10 09 Review By Age 3 neurogenin 3 Live M 2013 10 09 is amp z Recently Added Animals 10 l 10 ir 4 neuragenin 4 Live I 2013 10 09 Selected Animals 0 o A 5 neurogenin 5 Live F 2013 10 09 X Search Animals EID d teed tenetereue indeed amp neurogernin 6 Live F 2013 10 08 Measurements SF EN tga es eet REE EN NNI ANLAGE GA I 7 neurogenin 7 Live F 2013 10 09 Lab Events PEI AE pe EE ws pt es P e NE E B neurogenin 8 Live F 7013 10 04 Matings Litters UNE eS E e s Be en gp rp peer EU ERE OE ER IDE DOE EO pel ore pn pow E pepe m EA ma ranar Tii nra hl 32603 23 0 You can also select Showing All Projects and you will see all animals in your lab 62 Showing All Projects
33. Litter z Review Litters Review Litter By Status Active 5 uw Not Tagged 4 lal Not Genotyped 4 la Not Weaned 4 amp Weaned Selected Litters 0 i Mon mmal a re Note When you are done with a litter pups are weaned and you do not need to view litter information anymore change the Status to Not Active List of Litters Displaying 1 5 of 5 records Page 1 of 1 WF Add to Selection E No Litter m i neurogenin 1 m neurogenin 2 3 neuragenin E m 4 neurogenin A mo 35 Select an action Displaying 1 5 of 5 records neuragenin B Mating neurogenin E neurogenin 1 neurogenin 1 neurogenin 1 neurogenin 1 184 Mew Update Ty Delete Gl Export Date of Birth Tagging Date Genol 2013 10 11 2013 10 13 2013 10 13 2013 10 13 2013 10 13 2013 10 23 2013 10 23 2013 10 23 2013 10 23 Page 1 of 1 2013 ls Active ls Tagged ls Genotyped ls Weaned You will not see the litter anymore under Active Litters Do not Delete a litter unless it is an erroneous record Progress no Date of Birth Yes Tagging Date Yes Genotyping Date Yes Weaning Date 2013 10 13 2013 10 23 2013 10 28 2013 11 03 185 Trio Matings And Litters You can create trio matings one male vs two females and harem matings one male vs three or four females in mLIMS Make sure you record the litters for each female correctly the foll
34. Mating No of Sire per Mating Date Calculations Delivery Days Plug Days Cape Cards Animals Matings Litters Protocols Mo e g Sperm Injection 2 Maximum number of dam per mating up to 5 1 Maximum number of sire per mating up to 5 Yes gt Automatically fillin the plug date and delivery date 19 Default number of days from plug date If the date calculation is ei 1 Default number of days from mating date If the date calculation is 15 10 Litters tab Main Information Capes Cape Cards Animals Matings Litters Date Calculations Set to Yes Ei Date Calculatians Yes Automatically fill in the tagging date and weaning da Tagging Days and Weaning Days Tagging Days Default number of days from DOE If the date Leave Blank let each user set this up in their Projects Weaning Days Default number of days from DOE If the date 11 Protocols tab Main Information Cages Cage Cards Animals Matings Litters Protocols Is Total No of Animals Required Set to No unless you want to make Main Information entry of Total Animals Quota ls Total Mo of Animals Required Ho H mandatory see Create IACUC Protocol 12 Projects tab Main Information Capes Cage Cards Animals Matings Litters Protocols Projects Is Billing Account Required Projects ls Billing Account Required Ma 16 13 Drop Down List tab Main Information Capes Cage Cards Animals Matings Litters Protocols Projec
35. Search Cages 1i lal Cage Map F cage a Active 2013 10 male 7 Animals emmp Active 2013 10 CDF 5 11 F 567 Active 2013 10 neuro male 4 male 5 male 6 Lab Events 3 11 Matings Ir Active 2013 10 Sw 6 Sw 7 Sw 8B Sw 9 Sw Active 2013 10 Sw 1 5w2 5w 3 Sw 4 Sw 5 Litters Projects dis 1i 111 My Cages In top menu bar there is a shortcut button called My Cages If you click on this all your cages in all Projects will be displayed in one list HOME Hello Naomi Iwai LOGOUT Admin My Calendar My Animalg jnvironment Test showing All Projects Ja Search Browse rt Browse Deleted j Settings gT Print Cages 9 Create Cage List of Cages Displaying 1 100 of 154 records Page 1 of 2 P Review Cages rAddto Selection New Update Tj Delete Tj Show Deleted Records gl Export Event Review By Owner Recently Added Cages 13 Cage Report Mating Selected Cages 0 No Cage Name Type Status Setup Date MDA Cage A Search Cages B m 4 cage 33 Active 2013 10 CDF 6 a Cage Map 11 T E F 2 cage 23 Active 2013 10 male 8 male 9 Measurements 11i Moyet T F 3 cage8 Active 2013 10 CDF 10 Matings 11 TEASER TRUE TUER THEATER E 4 caged Active 2013 10 CDF 7 CDF 8 CDF 9 Projects 11 bi ena m5 cagea Active 2013 10 male 7 IACUC Protocols Bum ACCES mee ene meee ee eee 6 emmp Active 2013 10 CDF 5 System Settings Hn Users m 7 567 Active 2013 10 neuro male 4 male 5 rr
36. Tag Based On Abbreviation Abbreviation Animal Tag neurogenin Disable Auto Naming Based On Abbreviation Disable Auto Naming Based On Abbreviation neurogenin Disable Auto Naming ased On Abbreviation neurogenin 36 9 Cage Locations tab Set up your default cage location in this Project You will be able to choose from a drop down menu which was set up in Settings gt Drop Down List by your lab mLIMS manager If you don t have a specific location e g your mice are on several different racks leave the field blank 10 Mating amp Litter tab Set up your default number of Delivery Days Tagging Days and Weaning Days for this Project Delivery Days number of days from plug date to scheduled delivery date Tagging Days number of days from DOB to tagging Weaning number of days from DOB to weaning The system will create email reminders for delivery tagging weaning based on the number of days set up here If you do not want email reminders you can leave these fields Main Information Record Haming Building BSRB Room SB 8000 Rack Main Information Record Naming v Delivery Days V Tagging Days 7 Weaning Days 37 Cage Locations Cage Locations Mating amp Litter blank Even if you want email reminders you do not have to set up the number of days here you can do so when you create a mating or litter for more information refer to Section 7 Matings
37. Tag Based On Abbreviaton Abbreviation Animal Tag Pups Animal Name Initial field will be blank Animal Name Initial Toe Chip Mo of New Born g Select if you want to create a litter re You can enter an Animal Name Initial optional Animal Tag start number optional in this example the animal tag method is Toe Clip and the number of newborns 159 Pups Upon entering the number of newborns Animal Mame Initial neurosenin nuns neurogenin pups Toe Clip 21 The system will create a table with the number of pups you Mo of New Born 7 Select if you want to create a litter record without pups You can add more pups v selected Animal Name Status Toe Clip Sex It will name the pups based on the initial you chose you can leave the animal name initial blank and the system will assign numbers to the pups Set the first 1 iind Copy Auto Fill l The rest of the animals wi neurogenin pups 1 Live It will also auto fill the animal tag numbers based on the start number you chose you can leave the animal tag numbers blank and later fill in at the time of tagging neurogenin pups 2 Live neurogenin pups 3 Live neurogenin oups 4 Live 160 neurogenin pups 7 File Attachments Click here to attach files If you chose Animal Tag for R Main Information Record Naming Cage Locations Mating Animal Names S lactate Animal tJ Disable Auto Naming Animal Tag J Ba
38. ai X Search Matings 3 neurnaenin 777654 R 2013 13 27 2013 10 17 9 create amp Search 9 Lite 4 amp 7 Summary Breeding Cage Animals In Cage 2 Parents 2 Project Neurogenin 1 in brain tumor neurogenin 1 2013 09 26 2013 09 27 No 155 Sti Act been selected Once in Add Litter window and mating has been selected 1 Create Litter Name A litter name is mandatory and has to be unique in the lab there cannot be two identical litter names in the same lab 2 In Progress box enter Date of Birth by default DOB will be today Enter Tagging Date Genotyping Date and Weaning Date if applicable Entry of these dates is optional You may choose to leave them blank and later fill in when you perform these Main Information Main Information P Ji d t KT r aom L E Project Neurogenin 1 in brain tumor I Mating neurogenin 1 a About This Litter Ih pm i n Litter neurogenin 5 About This Litter DER am Litter neurogenin 5 Progress Is Active Date of Birth 2013 10 13 Yes No ls Tagged Tagging Date 2013 10 23 ls Genotyped No Genotyping Date No ls Weaned Weaning Date 2013 11 03 156 tasks But if you want email reminders you have to enter the dates Note that if you set up Tagging Days and Weaning Days in your Project see Section 4 Setting Up Your Projects the scheduled Tagging Date and Weaning Date are already p
39. and Litters Note Tagging and Weaning are set up as a pair you have to enter both days for the email reminders to work 11 Genes Order Tab If there are genes that you will use frequently in this Project drag drop them to the left Main Information Record Naming Cage Locations Genes To Be Displayed Neurogenin 1 FGFS 38 Mating amp Litter Genes Order Available Genes abe 12a cMyb DEF 4 H Ras Keratin 10 keratin 123 kras12 p27 In the example above Neurogenin 1 and FGF8 are the preferred genes in this Project Two things will happen a In Add New Animal window gt Genes and Genotypes field the preferred genes are displayed on top of the dropdown menu About This Animal Preferred Genes Neurogenin 1 Sex FGFB Animal Name uu Other Genes Status p27 Date of Birth p53a Coat DEF 4 Coat abc 12a Strain kras12 w me Keratin 10 Background Strain uw w cMyb Source PTEN w H Ras Purpose v keratin 123 Genes and Genotypes pos b In Review Animals gt List of Animals you can display the preferred genes as column header 39 List of Animals Displaying 1 10 of 10 records Page 1 of 1 Record p Add to Selection New Update J Delete Export Event 3 sacrifice Move Mating No Animal Name Sex DOB Genes amp Genotypes Neurogenin 1 aF8 e Dar 1 neurogenin 1 M 2013 10 09 Neurogenin 1 het 2 neurogenin 2 M 2013 10 09 Neurogenin 1
40. neurogenin 3 Live M X FGFB8 Neurogenin 1 2013 Click on Update button Review By Status null 09 the animal information window Selected Animals 0 neurogenin 4 Live M FGFS Neurogenin 1 het 2013 and click on Edit TO RE ee ee 88 Animal Information window displays Make your updates and click Save Main Information Cage Cage 1 Hokding M 4 F 1 l OWNEr Naomi wai l About This Animal Animal Name neurogenin 2 sex mae e Status riva m Date of Birth 2013 10 09 Coat v strain we Background Strain w Source ka Purpose w Genes and Genotypes Add More FGFs Lael e Remove Heuragenin 1 l mull ae Remove Wil click here to attach files Save Reset 89 Ear Punch Update Multiple Animals In Review Animals List select the animals that you want to update by checking the check boxes Click on Update button List of Animals Displaying 1 10 of 10 records Page 1 of 1 S FAdd to Selection New Animal Name neurogenin 1 neurogenin 2 neurogenin 3 neurogenin 4 neurogenin 5 neurogenin 6 neurogenin 7 neurogenin 8 neurogenin 9 Update T Delete J Show Deleted Reco Status Sex Live Live Live Live Live Live Live Live Live FGFS het FGFS null FGFS null FGFS FGFS het FGFS het FGFS FGFS het FGFS het 90 Genes amp Genotypes Neurogenin 1
41. the Selected Cages Animals same cage are also displayed You can select a different animal Please select the animals to be included in this mating from this table You can also add more animals to the mating in Ho Cages Animals Locations this example we will create a simple one male one female 1 777333 IV neurogenin 1g Building BSRB Room 56 8000 mating Cage 1 B amp 1 E gs29 C Building BSRB Room SB 8000 132 6 Select breeding cage Main Information oti Project Neurogenin 1 in brain tumor You can choose from existing 2 BILE LN x cages in this project or set up a new cage zr n Choose from all active Set up a new cage Note when you choose an Active Cages existing cage make sure it is a Breeding Cage Mating Details 777333 Holding M 1 F 0 777334 Holding M 0 F 4 Mating neut777335 Breeding M 0 F 0 Q Dam q 77400 Holding M 0 F 0 fF 7401 Holding M 0 F 0 7 Create Mating Name Mating Details A mating name is mandatory and Mating neurogenin 1 has to be unique in the lab there cannot be two identical mating names in the same lab 133 8 Enter Mating Date Mating Details Mating neurogenin 1 By default Mating Date is today Q Dam Q You can change it to any other Cf Sire Q Naomi Iwai Anicia Roberson date Email Notifications 4 Transfer the selected animals to this breeding cage David Cotten Debra Montague
42. to whom the event was assigned change the event status to Completed an email will be sent to all selected recipients notifying them of the update mLIMS lt do not eply mlims mdanderson org gt Ta Iwai Naomi Cc Subject mLIMS Event Update test event 17 2013 08 27 More Details Main Information Title test event 17 Start Date 2013 08 25 End Date 2013 08 27 Submitted By Naomi Iwai Assigned To Naomi Iwai Category Priority Normal Notes Selected Cages Animals Cages Animals Cage Locations event emails 5 event emails 3 216 Section 9 IACUC Protocols Review Protocol Information For how to create a protocol refer to Section 2 Setting Up Your mLIMS System Create IACUC Protocol In IACUC Protocols HOME Hello Naomi Iwai LOGOUT Admin My Calendar My Animals My Cages Environment Test Review Protocols you can z 3 sah z r Showing All Projects A Search wy Browse M Browse Delete Settings Print view all information 2 u 3 P related to your lab s List of IACUC Protocols Displaying 1 2 of 2 records Page 1 of 1 Animals Measurements o z v o Lab Events E Matings fC NGG F No TACUC Protocol Protocol Abbreviation Protocol Number Approval Date Expiration Date Total Ani Litters IF Drug injection Drug injection 22 444 777 2011 08 01 2014 08 01 50 Projects effect of chemicals a AE ILLIC ree E SE IRE HE ESO ADR S AD E diri on reproductive behavior IACUC Protocols F7 Study af br
43. when you move your cage and animals to someone else s Project remember to change the Owner of the cage and animals to the person who receives the cage Otherwise this person may not be able to use the cage and animals also see explanation in Section 5 Animals Move Animals showing All Projects Cages o Create Cage P Review Cages hk Review By Owner Recently Added Cages 13 zl Selected Cages 0 Search Cages 200 00 00 00 0 0 Ei Ei nv a Cage Map Animals Measurements Lab Events Matings Litters Projects Genes and Genotypes IACUC Protocols Billing Accounts System Settings a Main Information Cage 2 TITTI 765432 Main Information Project My Projects BRCA1 7 tumor observation Demo Project A Demo RISTS 6 13 EEE HHH Neurogenin 1 in brain tumor pos tumor observation Practice B Project B Project C xyz A Viii Chakravarthy Test Project Viji Anicia Roberson test protect 09 05 117 Cage Cards There are two cage card designs in mLIMS for Holding Cages and Breeding Cages Whether a cage is a Holding Cage or Breeding Cage is defined in the Purpose field of cages Holding or Breeding 1 In Review Cages List select List of Cages Displaying 1 5 of 5 records Page 1 of 1 the cages for which you ANE tO CERLE CAGE cards yrAddto Selection New Update 3 Discard Cages J Delete Project Cage Cards Jl by checki
44. 127 Add Female TO EXiSHnG MatinG sessessaet ie oa Ro E USE oU oi tinea hearse lesa p E VE EE Ror eR Ca a a M ay has EF IPLE LU CE a ahe ue a 142 ACG insimul LL E o LO a D DLL A E A A EG 153 update teer Tagging Genotyping Wednllitg s eaxeovsrtdatexdusda toxiuere orm biet oddrstodeadiwne torta esa teda toa bendi Cox bredurovtebPttodbrpten os dust Cordubd E Data 168 goduidinis fipsEini Sc ETT LO TL LIII M 186 Complete Or Cancel MAUI G esses videsseasamdwriatostater aont tbe da vate VPsbn ves aad van ela vieles at s rd EE AE Afra sam a pda fequi atn 194 Mating eC LRE EMAI NOCIONS om I Em e 195 Section 8 Lab Events CS a NENEVE I CERTE PEUT CPI hi resect tc ware ae ena nance ar ie kts eee ant he ace oa sa mae aut ule Game aceasta nae Gaius eae amntenate 203 RE VIC Ws E VENTS cuiua eas uie dM ipM ULL ube nade ene MID MEL Mu A D MUR M gare MM d DM ME LEM UE iM E 211 Event mE cM IIENNLOS NICO TOES 213 Section 9 IACUC Protocols REVIEW Protocol TMFORMMAT OM ETT m 217 Renew Protocol Section 1Getting Started With mLIMS Log in to mLIMS 1 www2 mlims com 2 Enter your User Name and REGISTER LOGIN Password and click on Sign In Sign in with your MD Anderson account User Name Password If you cannot access your account please contact your support at MD Anderson The mLIMS Home Screen In the home screen NOME Helo saami wo TE
45. 13 783455 xyzA window or new tab depending on A 06 your browser settings 27 If you choose Same Window the information will display in the same window 30 View Details open as a standard page from now on Main Information Cage 83455 IACUC Protocol Billing Account Project xyz A Owner Naomi Iwai About This Animal Animal Name 1 2 Sex Male Identifications Status Live Age 3m DOB 2013 06 27 Coat Strain Background Strain Source Purpose Genes and Genotypes 3 Select your preferred option and click Save Section 4Setting Up Your Projects 31 Create Project It is important that you create a Project before you start entering data A Project is a group of animals and cages and related information Users can create multiple Projects based on how they want to categorize their animals cages e g per research project per mouse strain Always create a Project first then create animals cages under the Project 1 In left hand menu select Projects Showing All Projects gt Create New Project Main Information Record Haming Cage Locations Mating amp Litter Genes Order Users Cages E Sede ARI EM US E PET oe cee ae RT sary m Matings Litters Click here to attach files z Review Projects t4 Search Projects gt Genes and Genotypes gt IACUC Protocols gt Billing Accounts System Settings quide TUER uh Help 32 2 Enter Proje
46. 8 Live F 2013 10 Cage Holding FGFS Neurogenin 1 09g 2 het neurogenin 9 Live 2013 10 Cage Holding FGFS Neurogenin 1 09 2 het neurogenin 10 Live h 2013 10 Cage Holding FGFS Neurogenin 1 78 3 Move Animal window i ee ae Main Information displays Animals To Be Moved 2 neuragenin 6 neurogenin 7 Main Information The system will take the protocol information from the target cage and apply the animals Choose from all active Set up a new cage 79 You can move the animals to an iia fornai existing cage all active cages in the current Project display The system will take the protocol information from the target cage and apply the animal Cage amp Choose from all active cages O Set up a new cage cage a Breeding M 1 F 2 Cage A12 Holding M 3 F 0 Cage 5 Breeding M 1 F 2 Cage 6 Holding M 0 F 3 Reset cage 232 Holding M 0 F 1 7000000 Breeding M 1 F 3 9000000 Breeding M 1 F 3 15 Cage 7 Holding M 0 F 3 Cage B Holding M 4 F 0 6662223 Holding M 0 F 0 5000001 Holding M 0 F 0 A Holding M 0 F 1 80 Or you can set up a new Cage Create Cage window displays Enter cage information and click Save Main Information Project Neurogenin 1 in brain tumor Owner Naomi lwai About This Cage Cage Name MDA Cage TACUC Protocol brain tumor 99 11 2346 Billing Account 11 22222
47. A Purpose Holding Type Setup Date 2043 10 14 Notes Cancel 81 Building Room Rack Row Column A new cage has been created Click Save Don t forget to click on Save or animals will not be moved Note You can move animals to a cage in the same Project or to a cage in a different Project To move to cage in same Project Make sure that the Project is selected in Projects drop down menu Cages in this Project will display in Choose from all active cages Animals To Ac 2 neurogenin 6 neurogenin 7 Main Information The system will take the protocol information from the target cage and apply the animals af A cage has been created Cage 7693832 If you choose to set up a new cage this cage will be created in the selected Project To move to cage in different Project Select the target Project in Projects drop down menu Cages in this target Project will display in Choose from all active cages If you choose to set up a new cage this cage will be created in the selected target Project Neurogenin 1 in brain tumor Cages Animals 9 Add New Animal 9 Add Multiple Animals z Review Animals hk Review By Owner P Review By Status hk Review By Age Selected Animals 4 Search Animals Saved Searches Measurements Lab Events 83 Main Information Animals To Be Moved 2 neurogenin 6 neuragenin 7 Main I
48. FGF8 to FGF9 in all existing records FGF8 will be changed to FGF9 Note you can remove a genotype from a gene only if that genotype has not been used yet Once a genotype has been used in an existing record see below the genotype cannot be removed you will not be able to check the Remove check box 25 Main Information Gene PTEN e g Pten Genotypes Check to remove the genotypes from this gene Genotypes Usages TES 26 Create IACUC Protocol The entry of IACUC Protocols is mandatory in mLIMS Please consult with your PI or manager and obtain accurate information about your protocol before starting It is highly recommended that permission to the IACUC Protocols tool is restricted to the lab mLIMS manager only The lab mLIMS manager should be responsible for accurate entry of protocol information including renewals 1 In left hand menu select IACUC 5 HOME Hello Naomi Iwai LOGOUT Admin My Calendar My Animals My Cages Environment Test Protocols Create New Protocol Showing All Projects B Main information s I amp CUC Protocol Title Animals IUD Measurements Protocol Abbreviation Lab Events u Protocol Number Matings Approval Date 2913 09 24 Litters eae Expiration Date Projects k Genes Genotypes Tatal Animals Quota JACUC Protocols Notes F Create New Protocol Review Protocols z Selected Protocols 0 Click here to attach files t Search Protocols Billing Accounts
49. IMS Event Create Test event 8 27 1 Drug Treatment 2013 08 25 Exception when the event Start Date is today or a past date no More Details email is generated Main Information Title Test event 8 27 1 Start Date 2013 08 28 End Date 2013 08 28 Submitted Bv Naomi Iwai Assigned To Naomi Iwal Category Drug Treatment Status New Priority Normal Notes test Event Reminder 214 mLIMS sends an Event Reminder mLIMS lt do not eply mlims mdanderson org gt email to selected recipients on the To Iwai Naomi Start Date and End Date Cc Subject mLIMS Event Reminder test event 1 2013 08 27 If the Start Date and End Date are the same one time event only one email is generated on this day More Details The email is sent at 12 00 am Main Information Note when the Start Date is today Tille test event T7 or a past date at the time of event Start Date 2013 08 25 creation no email is generated End Date 2013 08 27 Submitted By Naomi Iwal Note if the Status of the Event is Completed or Canceled no email is E generated Category Status New Priority Normal Assigned To Naomi Iwai Notes Selected Cages Animals Cages Animals Cage Locations event emails 5 event emails 3 Event Update Notification 215 When you make an update to an event mLIMS sends an email to the selected recipients immediately to notify them of the event update For example if you or someone else
50. No of Dam per Mating in System Settings Settings Matings is set accordingly 152 The system will not allow matings with multiple dams if the number of dams exceeds the maximum number set up in Settings showing All Projects Main Information Cages Cage Cards Animals Matings Litt Matings Animals Allow Female Only Mating e g Sperm Injection Ho of Dam per Mating Maximum number of dam pei b b p Measurements MEME PE PEE ee esee tue tcs eit Matings No of Sire per Mating Maximum number of sire per Date Calculations Yes Automatically fill in the plug date ar Ld d DURS NEED MNT MEER E RR Delivery Days 24 Genes and Genotypes le p Default number of days from plue date If th IACUC Protocols ERRORI CEP RU UTER RE Plug Days 4 Billing Accounts Default number of days from mating date If Settings Additional Information LJ llcarc Darmiccinne 153 Method 1 In Litters select Add Litter In this method you will have to select the mating from the magnifying glass icon list Method 2 Select a Mating first and add litter In Matings gt Review Matings Select mating by checking the check box then click on Litter button OR Click on the mating name which kA Meurogenin 1 in brain tumor Measurements Review Litters Selected Litters 0 t Measurements Lab Events Matings 9 cet Up Mating hk Review Mating By Status Sele
51. Plu mating so that when pups are born the system knows who the father is ls trio mating male 13 female 7 female 8 Active 10000 2013 10 14 201 Animals do not have to be in the same cage to be part of a mating 187 2 Add Litter to female 7 Mus kalin Go to the mating and select Add l l Litter see above Add Litter and Main Information Pu S beeper t 22 s s da i ps Project Heurogenin 1 in brain tumar About This Litter Litter female 7 pups v r TT kd eee al I In Pups table note that there is a column Dam 1 This is because there are two dams in this mating and you will have to select the mother of this litter 188 Pups Animal Name Initia trig Toe Clip 1 Selectfemale 7 as dam No of New Born m B Select if you want to create a litter record without pups You can add more pups when editing this Ho Animal Mame Status Toe Clip Sex Set the first 1 i animal The rest of the animals wil 1 triol we x 1 Im al Actions Copy Auto Fill Copy 2 trio lve r 2 im 1m 3 trio3 Live Iz 3 im 2 jx Note that in Cage column the original breeding cage 10000 is selected 189 Ho Animal Hame Status Toe Clip Set the first The rest of the t iu Live I 1 female 7 v Actions Copy Auto Fill 2 trio Live 2 Change this to the correct cage where female 7 and litter reside 3 trio Live 3 female 7
52. Plug Date and Delivery Date will Ni v Verify that there are no other animals living in this breeding cage be auto calculated based on the Mating Date 2013 09 26 Mating Date you chose and the Plug Date 2013 09 27 number of days to Plug and Is Pregnant No v L Nina Chang System Manager Delivery in your settings default number of days to Plug should have been set to 1 by your lab mLIMS manage in Settings You can set up the default number of Status Active days to Delivery in your Project Email Notifications yes v settings Notes Jane Doe Nami Hei Haomi Test Natalie Chang Pregnancy Date 7 viji Chakravarthy Delivery Date 2013 10 16 Retire Date You can change these dates to any other date in this window You can also enter Retire Date the date you plan to discontinue this mating 134 9 Set up Email Notifications mLIMS will send you email notifications for Mating reminders Delivery reminders and Retire reminders if you don t want emails for this mating select Email Notifications No In the Email Notifications box select the email recipients You can choose any of your lab members For more information on email notifications see below Mating amp Litter Email Notifications 10 Click on Save Mating Details Mating sa Q Dam C Sire Mating Date Plug Date ls Pregnant Pregnancy Date Delivery Date Retire Date a Status Email Notific
53. Review By Owner Caae 1 Discarded 2013 10 09 115 Move Cage To Different Project You can move a cage and its animals to a different Project 1 In Review Cages List select Neurogenin 1 in brain tumor 29 Search quy Browse 7 Settings ME Print the cages that you want to cages move by checking the create Cage List of Cages Displaying 1 11 of 11 records Page 1 of 1 check boxes r Review Cages e E at i i Acti 11 Add to Selection New 4 Update J Discard Cages j Delete 2 Click on Project Active 11 i Breeding 0 wl Holding 11 567 Active Holding 2013 10 neuro male 4 Naomi la Discarded 1i male 5 male 6 Iwal No Cage Name Status Purpose Setup Date Animals Ownei Review By Owner v 765432 Active Holding 2013 10 fem2 Naomi Recently Added Cages 12 11 Iwal E SMOE S ages 10 777777 Active Holding 2013 10 CDF3 CDF4 Naomi Search Cages 10 Iwal x cuin oe m Cage 2 Active Holding 2013 10 neurogenin 8 Naomi 09 neurogenin 10 Iwal Animals Measurements F Cage 3 Active Holding 2013 10 5w 1 5w 2 5w 3 Naomi i 11 Sw 4 5w5 Iwal Lab Events 116 3 In Move Cage to Different Project window select the target Project 4 Click Save Cage plus animals in the cage will be moved to the target Project Note IACUC Protocol and Billing Account information for the cage and animals will not change upon moving to different Project Remember to update if necessary Note
54. Search Animals V ea NORRIS ee EET 134 Live 2013 06 27 P eee A i Live 2013 09 01 Lab Events Pad ar e IDEE SERERE QURE ERE UNE D REED TE RD A TE GR DT DU BRCA female 1 live 2013 04 18 Black 3 3 4 5 o Fi 8 z Matings RE DA EU EEG ERU or Ur Wr ESE EEE SAE ge ge rr T We WI Eur EXE Ur E RU UP KU rar BRCA female 2 Live 2013 04 16 yellow Litters i EA Demo 4 1 Live 2013 08 12 Black E pue Projects Il Il Demo A 2 Live 2013 08 12 Agouti 99 2 Click on Search button l e showing All Projects LE search Y Browse qj Browse Deleted 7 Settings Print List of Animals Displaying 1 97 of 97 records Page 1 of 1 Animals 9 Add New Animal tee IR al i m 9 Add Multiple Animals Add to Selection New 4 Update J Delete J Show Deleted Records Review By Owner F 17 Live 2013 06 27 Review By Status F 13 Live 2013 06 27 Review By Age F 3 14 Live 2013 06 27 Recently Added Animals 10 Selected Animals 0 30 Live 2013 06 27 2i Live 2013 06 27 Search Animals E v UY UY VEU E Y UY E 134 Live 2013 06 27 E E A 1 Live 2013 09 01 Lab Events BRCA female 1 Live 2013 04 16 Black Matings Litters Demo 1 Live 2013 08 12 Black Demo 2 Live 2013 08 12 Agouti Genes and Genotypes Demo A 4 Live 2013 07 24 Black A Lo CES 0 LOS EOS 00 O0 EOD 2X IACUC Protocols Demo Live 2013 07 24 Black 100 3 Set up the search criteria Search Status ls
55. ShODDIhug dl eios rrt aded v xex dU Vox dud vs aene dtd eee te ste eT rer rete ee Terre te EE AE FIM EM MM IDEEN M PME 68 BINE ISTE ETT E UMu M Ed 71 SVG MIS PANN VNU E TIO T T E eme 75 MOVES APIA aches COCOA tem 78 ReviewAnitmmalPedigree andAnInCeSbt y odis iid deere qudd tide b t qd edd iawbda tke d ped erdt nr nc get ea uU metre a mea ceed me rover sane A 85 Update Animal InFotfriatlOPis sensisset uA bIOPEPES EOS RHUA M IquWRERA E SF ERE NOTE ERREUR NIIRRRETE DID INADISEEISE RED NUMINE ES RA DNUS EP EUM S Fa pU RIS 88 mint re IET MNT ETOILE EDO SP Pe RE RSE RECO SA PETE EE ORT R P aC eT 94 Search R cords and Save Searches caus cee scercwvetines A che de trira ated stan aide Xu A aan dba PITE Rada ue abe DI E LM LAT LM PPM CM ILE 97 Section 6 Cages gcc dil rc Pr 108 REVIEW GAGES PEE 111 EVES Cai CC OCS os ETT TI D desea wee Sea eink ok veg ten sng Sena HT AeA Hw ats nO Pes sb naan dd ed ae A es 114 Move Cage TO DIMerent iPro CCl c tao P bo d Pa dC dee t ated Ua ato combat De taa cL Pa D OE EC M Eu e TRI EM C A PM M M ELA CUM PUn M d bae C EIC T NO E 116 lof roc LE E 118 Cage REDON me PUE uuu 121 cage clos 123 Section 7 Matings and Litters OCUPA A a toc tua tied vacate soniye ie Cid otpi gol vse pare ol am ME T ENDE bM CN gl MINI DEI seme gl uma ee ou EON E
56. Weaned Notes mLIMS Litter Weaning Reminder Test litter 6 286 1 2015 09 01 Test litter 8 28 1 2013 08 29 2013 08 30 2013 08 31 2013 09 01 Yes No No Additional Information Strain Background Strain Mating and Litter email generation rules e Emails are sent on future dates only i e tomorrow or later They are not sent on today Example It is 9 26 today and set up a mating 199 Mating Details Mating neurogenin 3 Q Dam Ci Sire 4 l 7 Transfer the selected animal sf Verify that there are no oth Mating Date 7013 09 76 amp Plug Date 9973 09 27 Ils Pregnant Wo Pregnancy Date Delivery Date 2013 10 16 Retire Date Status Active Email Notifications yo The Mating Date is today Iwill not receive a Mating reminder Example It is 9 26 today and I set up a mating with Mating Date 9 30 perhaps as reminder for myself or others to set up this mating in the future 200 Mating Details Mating neurogenin 3 as Q Dam A i Cj Sire A Transfer the selected animals 7 Verify that there are no othei Mating Date 2913 09 30 4 amp Plug Date 2013 10 01 Is Pregnant py Pregnancy Date Delivery Date 2013 10 20 Retre Date Status Email Hotificatians Yes will receive a Mating reminder on 9 30 e You can edit the mating or litter at any time and change dates and email recipients The system will update
57. age location options Buildings Rooms Racks for the whole lab especially if you want to use the Cage Map feature see Section 6 Cages Cage Map Background Strain Add More mixed Black 6 Please select a category you like to edit Building B You can define your category list here If you like to re order the values please select tl Notice that if you delete a category the selected category will be disappear from your Building Add More ESRB 19 Please select a category you like to edit Room M You can define your category list here If you like to re order the values plea Notice that if you delete a category the selected category will be disappear f Room Add More 58 8000 51 8888 20 Please select a category you like to edit Rack You can define your category list here If you like to re order the values please Notice that if you delete a category the selected category will be disappear fro Rack Add More Additional Information fields In most tabs you will see a section called Additional Information 21 Additional Information No Name Type 1 Text Single Line v i Text Single Line 3 Text Single Line 4 Text Single Line ka 1 Here you can define your own custom fields if there is data you would like to record that is not already available in the system Example Additional Information Ho Hame Type 1 Animal Order Dat
58. ain brain tumor 11 222 333 2013 03 01 2016 03 01 PN tumors and the effect of surgery in genetically modified mice create New Protocol Selected Protocols 0 t Search Protocols Select an action Displaying 1 2 of 2 records Page 1 of 1 mLIMS will track the List of IACUC Protocols Displaying 1 2 of 2 records Page 1 of 1 number of animals and cages per protocol x rAddto Selection New Update Tj Delete J Show Deleted Records z Export Renew No IACUC Protocol Protocol Abbreviation Protocol Number Total Animals Quota No of Total Animals No of Active Cages 1 Study of brain brain tumor 11 222 333 100 14 4 tumors and the effect of surgery in genetically modified mice 217 How mLIMS counts the number of animals per protocol is not consistent with IACUC regulations mLIMS does not give you a warning when a protocol expires nor when you have exceeded the total animals quota For accurate information and status of your protocol please contact appropriate parties outside mLIMS Renew Protocol 1 In Review Protocols Showing All Projects w9 Search amp J Browse m Browse Deleted yf Settings Print List check the check LIT box for the protocol psp mM List of IACUC Protocols Displaying 1 2 of 2 records Page 1 of 1 Animals Measurements that you are going to senem E z new 7 Update J Delete J Show Deleted Records Export Renew Lab Events aad Matings m No rACUC Protocol Protoco
59. ale 2 Female 3 neuro 1 neuro 3 neuro 7 neuro 8 neuro 9 neurogenin 8 Live Live Live Live Live Live Live Live Live Live Live Live Live Live 2013 10 11 2013 08 06 2013 08 06 2013 08 20 2013 10 11 2013 08 20 2013 08 21 2013 09 03 2013 10 12 2013 10 12 2013 10 12 2013 10 12 2013 10 12 2013 10 09 Displaying 1 15 of 15 records Page 1 of 1 146 Naomi Iwai Naomi Iwal Naomi Iwal Naomi Iwal Naomi Iwal Naomi Iwal Naomi Iwal Naomi Iwal Naomi Iwal Naomi Iwal Naomi Iwal Naomi Iwal Naomi Iwai Naomi Iwal Neurogenin 56 Bree Neurogenin 56 Bree cage a cage a 7000000 femi Cage 6 Cage 5 Cage 5 Cage 6 Cage 6 Cage 6 7 Note that the females have been added 8 Click Save 9 Click on Please click here to review your record Main Information Selected Cages Animals No Cages Animals Locations 7 000000 Maleg M Female 19 Building Main Information Cage 7000000 Mating Details Mating Test Mating 1 i Q Dam C F Female 1 Female 2 W Female 3 Cj Sire A V Male Mating Date 2013 10 17 Plug Date yw The data has been saved Please click here to review your record 147 10 Note that although the females have been added to the mating they reside in Generation different cages Expected Number of Pups 0 Female 2 and 3 still reside in their Dams original cages 5 5 Cage Animals Ca
60. als 124 W Search Jy Browse Browse Deleted j Setting F w mi y g List of Cages Displaying 1 100 of 175 records Page 1 Add to Selection New f Update E Delete IE Sh Cage Report Mating No Cage Name Type Status Setup Dz 2013 10 il 1 cage 33 Active Active 2013 10 11 cage 23 2013 10 1i cage 8 Active cade d Active 2013 10 2 Select Building Room and HOME Hello Naomi Iwai LOGOUT Admin My Calendar My Animals M Rack fom Building BSRB Roo mz Rack 125 Cage Map displays for the rack Building Room Rack Cage 5 RISTS of Animals 2 cage B RISTS of Animals 1 Q m 765432 RISTS of Animals 1 O cage 23 RISTS of Animals gf ee Section 7Matings and Litters cage a RISTS of Animals 1 g e cage 33 RISTS of Animals 1 Q m 567 RISTS of Animals 4 cage d RISTS of Animals 3 emmp RISTS of Animals 1 Q m Cage 4 RISTS of Animals 5 CG m amp 4 Cage 3 RISTS of Animals 5 Set Up Mating There are three possible ways to set up a mating Method 1 Create a mating from List of Animals Displaying 1 15 of 15 records Page 1 of 1 Review Animals List j rAddto Selection New Update J Delete J Show Deleted Records Export F Event 3 sacritice move mating In Review Animals List select dam and sire and click on Mating No Animal Name Sex DOB Cage Backgroun
61. als Export use this button to export your records to Excel Event see Section 8 Lab Events Sacrifice see below Sacrifice Animals Move use this button to move animals to a different cage see above Add Multiple Animals and below Move Animals Mating see Section 7 Matings and Litters Note you can change the order of these buttons by dragging them left and right The same actions are also available at the bottom of the Review Animals List 65 ua F 43 pup Live M 2013 Cage 8 Holding 10 44 pup Live M 2013 Cage amp Holding 45 pup Live M 2013 Cage 8 Holding 46 pup Live F 2013 Cage 7 Holding 47 pup Live F 2013 Cage 7 Holding 48 pup Live F 2013 Cage 7 Holding 49 pup Live M 2013 Cage 8 Holding Select an action Displaying 1 49 of 49 records Page 1 of 1 66 LP F pup Live M 2013 Cage 8 Holding 10 13 44 pup Live M 2013 Cage 8 Holding 10 13 45 pup Live M 2013 Cage 8 Holding 10 13 46 pup Live F 2013 Cage 7 Holding 10 select an action 13 r Add to Selection Live F 2013 Cage 7 Holding New 10 Update 13 i Delete Live F 2013 Cage 7 Holding show Deleted Records 10 Export 13 E Event Live M 2013 Cage 8 Holding sacrifice 10 Move 13 Mating Select an action T Displaying 1 49 of 49 records Page 1 of 1 67 The Animal Shopping Cart This feature allows you to select animals that you want to temporarily group together for a mating ev
62. ation choose from existing cages make Project sure you choose a Breeding Cage ee Choose from all active cages Heurogenin 1 in brain tumar I J Set up a new cage 3 Create Mating Name Mating Details z u i mj Mating neurogenin 3 a M m n 139 Select Dam by clicking on magnifying glass icon This will display a list of all females in this Project Select your dam s by checking the check box es and click on Select Mating Details Mating S Q Dam s Cj Sire Mating Date Dhan Nis te neurogenin 3 a ON UM V Transfer the selected animals to this breeding cage Verify that there are no other animals living in this bree 2013 09 26 S840 Fark MUT 140 AL gg FULL In I PLP ey 09 26 2013 777888 d Holding pa 26 2013 77 888 1 Holding 09 26 Swiss 7 2013 09 26 2013 09 26 Swiss 9 2013 pa 2b Swiss 10 2013 09 26 Displaying 1 14 of 14 records Page 1 of 1 5 Select Sire the same as above Mating Details Mating neurogenin 3 Q Dam Q Mies 3 i J 1 T Cj Sire 4 F neurogenin 4 El Transfer the selected animals to this b m Verify that there are no other animals 6 Follow steps 8 11 in above Create a Mating from 141 Add Female To Existing Mating You can add more dams to an existing mating To do this follow two steps 1 add the dams to the mating and 2 move the dams to the breeding cage 1 Selec
63. ations Motes neurogenin 1 oa Me ia Transfer the selected animals to this breeding cage v Verify that there are no other animals living in this breeding cage 2013 09 26 2013 09 27 No v 2013 10 16 Active Yes 135 a Iv Email Notifications Naomi lwai David Cotten Jane Doe Haomi Test Hina Chang viji Chakravarthy Anicia Roberson Debra Montague Hami Hei Hatalie Chang System Manager 11 A Summary of your mating Mating Edit Create amp Search Litter will display Summary Breeding Cape Animals In Cage 2 Parents 2 Audit Trails 1 It is good practice to go through each tab Breeding Cage Animals Lab Information in Cage Parents and make sure Project Meurogenin 1 in brain tumor that your mating is set up Cage 777335 correctly Mating Details Mating neurogenin 1 Mating Date 2013 09 26 Plug Date 2013 09 27 ls Pregnant Mo Pregnancy Date Delivery Date 2013 10 17 Retire Date Status Notes Additional Information Strain Background Strain Category Create a Mating from Review Cages List 136 1 Select in left hand menu 99 seach ij arewes fo serine MS Pont Cages gt Review Cages Lict of Capes Displaying 1 7 of 7 raccede Paga 106 1 remate Cage Ruviuw Cages Jahid Smee Gunite C pese fleet Feet oo Capecants CiProec C Decanbcagen Cage Rencet mating Wi Brewing 2 Me Cepe hamo Type Simus Setup Da
64. ave the same name If you leave it unchecked the system will auto name the animals will add numbers behind the initial so that all animals will have different names Note you will not see this Naming Rule field option if you chose No for Allow Duplicated Animal Name in Settings gt Animals tab In this example we will leave this check box unchecked Main Information Project Automatically Create Cages amp CUC Protocol Billing Account Ho of Animals Animal Mame Initial Haming Rule Neurogenin 1 in brain tumor I Uncheck this option if you prefer brain tumar 123 444444 H All animals have the same name Fr 51 8 Enter animal information and click Save Animal Mame Initial pg Animal Information Sex Date of Birth Female 2013 09 26 Coat Strain Background Strain Source Purpose Genes and Genotypes Add More Q Dam Oo Sire Notes 52 9 A message will appear the data has been saved 10 Click on here to review the animals The data has been saved Please click here to review the animals 53 11 You can view the animals Review Animals No Animal Name Sex DOB Cage Background Strain Gene that you created Review By Owner 1 Bel F 2013 Cage 09 1 Review By Status 3 Note that the system has Review By Age numbered the animals so that e 2013 Cage Recently Added Animals 13 de the an
65. be MDA Cape amp of Animals Animas Billing Accessit Building Gate Discarded Ewe HI y mom m M or SA l 17333 Adha 2049400 ESFE il Discarded 25 E Repne By erer Aetently Saipe cages Hi 2013 neurceram zd z Sev zH Selected Coes 0 Ewism 2 See E sapih Cages 213 0 naufcosran iM Coe Map 25 i 46 i Lora Maas ureimeits F ee Uere Lab Events nena Ming J Litters Projects WesWg Ana 2013 3 25 2 In Review Cages List select List of Cages Displaying 1 7 of 7 records Page 1 of 1 the cages that contain dam and sire by checking the yrAddto Selection New f Update J Delete export Event CageCards gt Project J Discard Cages CageReport Mating check boxes No Cage Name Type Status Setup Date MDA Cages of Animals Animals Billing Account Building Date Discarded Evel 3 Click on Mating button 1 777333 Active 2013 09 0 BSRB 25 777334 2013 09 d neurogenin 25 2 Swiss 1 Swiss 2 Swiss 3 777335 2013 09 2 neurogenin BSRB 25 1 B6 1 777400 2013 09 neurogenin 25 3 neurogenin a 777401 Weaning Active 2013 09 Z3 137 4 Set Up Mating window will display Main Information Selected Cages Animals Choose your desired dam and sire by checking the check boxes Please select the animals to be included in this mating No Capes Animals Locations 7 7334 neurogenin 29 Swiss 1 Swiss 29 Swiss 3 9 Building BSRE Room 56 8000 TT F400 neurog
66. ct name 3 Select a default protocol for this Project Animals and cages that you create in this Project will by default have this protocol number 4 Select a default billing account for this Project Animals and cages that you create in this Project will by default have this billing account Note You will not see this field if you have not created a Billing Account 5 Click Save Main Information Project Protocol Billing Account Motes Record Naming Cage Locations Mating amp Litter Neurogenin 1 in brain tumor brain tumor I Click here to attach files Tip keep your Project name short as it will appear in the Projects selection menu 33 Genes Order Users Shoni I J Search wey Browse b Browse Deleted Settings Print Showing All Projects 3 et T P g g List of Animals Displaying 1 50 of 101 records Page 1 of 3 0312 after data migration BRCAT 2 tumor observation Demo Project A Demo RISTS 8 13 Add to Selection New i Update J Delete J Show Deleted Records Ex FEF E HHH NO Animal Name Sex DOB Cage Project Background Strain namio Pi 1i2 M 2013 783455 xyzA Neurogenin 1 in brain tumor D6 Oscar Testing 2 27 p53 tumor observation ma i3 M 2013 783455 xyzA Practice B Eat D6 2 Project B 27 Project C m3 14 M 2013 783455 xyzA i Project D T D6 COS e A j Vr Charrawirhy Fja4 30 F 2013 PracB Practice B ef Test Project Vip
67. cted Matings 0 C Search Matings 154 C No mating ua Main Information Main Information Project Neurogenin 1 in brain tumor I Mating Q o About This Litter Litter neurogenin 1 Email Notifications ye Notes 29 Search wu Browse ri Settings Print List of Matings Displaying 1 3 of 3 records Page 1 of 1 x rAddto Selection New Update J Delete E Export Litter Cage Parents Mating Date Delivery Date neurogenin 777335 neurogenin 2013 09 26 2013 10 17 1 1 B6 1 neurogenin 777333 neurogenin 2013 09 26 2013 10 16 2 2 neurogenin 3 neuroadenin 777654 RA I 2 2013 09 77 2013 10 17 takes you to the Mating Neurogenin 1 in brain tumor 29 Search an Browse j Settings g Print Information window Click on Litter button Horns E SE SE Matings 9 set Up Mating Mating Edit Cage 777339 Mating Details This way the mating has already Cages Measurements Lab Events Lab Information Mating Mating Date Plug Date ls Pregnant Pregnancy Date List of Matings Displaying 1 3 of 3 records Page 1 of 1 x rAddto Selection New i Update lj Delete E Export Litter Fr No mating Cage Parents Mating Date Delivery Date 1 neurogenin 777335 neurogenin 2013 09 26 2013 10 17 ES Review Matings 1 B6 1 2 neurogenin 777333 neurogenin 2013 09 26 2013 10 16 hk Review Mating By Status 2 2 Selected Matings 0 ia
68. d Settings g to perform the same search v click on Saved Searches in left hand menu Animals 9 Add New Animal 7 Add Moltiblo Arimak SrAddto Selection New Update J Delete J Show Del iB Review Animals No Animal Name Status Sex Genes amp Genoty List of Animals Displaying 1 97 of 97 records Page 1 of 1 P Review By Owner E i 17 Live I Review By Status 13 Live M Review By Age Recently Added Animals 10 F 14 Live Selected Animals 0 30 Live L anumals Saved Searches I 31 Live t Measurements m A nra 106 11 Click on the search and the results will be displayed at once showing All Projects 49 Search eu Browse ri Browse Delete List of Animals Displaying 1 97 of 97 rec Animals 9 Add New Animal 9 Add Multiple Animals Add to Selection New Update J Review By Owner 12 live M Review By Status 13 Live M Note you can rename or delete Review By Age a saved search in the Smart Recently Added Animals 10 F 14 Live Search window Selected Animals 0 30 Live Search Animals Saved Searches m 31 Live ll PTEN animals 134 Live Section 6Cages 107 Create Cage You can create a cage and later move animals into this cage 1 In left hand menu select Neurogenin 1 in brain tumor Cages gt Create Cage Cages Note Make sure a Project is selected h Review Cages k Review By Owner Recently Added Cages 2 Se
69. d Strain Genes amp Genotypes Status Age Cage Purpose Cage Type Coat Ear Tag button J 1 B61 F 2013 Cage Live Od Holding 09 1 26 2013 Cage Live d Holding 09 1 26 2013 Cage Od Holding 09 1 20 J 4 neuragenin 1 M J013 777333 Black 6 Non 1 PTEN Live d Holding Agouti 1 09 4 D 22 127 Method 2 Create a mating from i List of Cages Displaying 1 7 of 7 records Page 1 of 1 Review Cages List i i JFAdd to Selection New i Update fy Delete H Export Event Cage Cards gt Project J Discard Cages Cage Report mating In Review Cages List select cages x that contain dam and sire and No Cage Name Type Status Setup Date MDA Cages of Animals Animals Billing Account Building Date Discarded Eveli click on Mating button 1 77333 Active 2013 09 0 BSRB 225 J 334 2013 09 4 neurogenin BSRB 25 2 Swiss 1 Swiss 2 Swiss 3 777335 2013 09 2 neurogenin 20 1 B6 1 400 2013 09 2 neurogenin BSRB 25 3 neurogenin a 777401 Weaning Active 2013 09 25 128 Method 3 In Matings select Set Neurogenin 1 in brain tumor e a a Up Mating Cages i pe o aC Main Information Animals sussssrrussusrusssrusesumssunssuerusssasusssurcum Project 2 2 Mos Rs Neurogenin 1 in brain tumor ix Lab Events p 1 Choose from all active cages i Set up a new cage Matings gt Set Up Mating Active Cages Review Matings Mating Details H Review Mating By Status Selected Mating
70. date In this example checked Coat If choose Black and save all selected records displayed on top will be changed to coat color Black Records To Be neurogenin 1 neurogenin 7 neurogenin 3 neurogenin 4 neurogenin 5 neurogenin 6 neurogenin 7 neurogenin B nei Updated 10 neurogenin 10 Please check the box next to the data field first before editing the value If the box is unchecked no change will be made to the value Main Information Owner Sem Status Date of Birth Coat Strain Background Strain Source Purpose Naomi Iwai Unknown Live Punch Black Agouti Tan White Albino gray Genes and Genotypes Help To Update Multiple Cages there is only one option available All selected records will have the same values Bulk updates are not available for any other records such as matings and litters You will have to choose one record and make an update 93 Set Up Column View You can choose what columns to display in your Review Animals List The following instructions apply to all Lists such as Review Cages List Review Matings List Review Litters List etc 1 Click on Settings button Neurogenin 1 in brain tumor 9 Search nj Browse j Browse Deleted Print Cages Animals 9 Add New Animal E o le Li Ex 9 Add Multiple Animals T Add to Selection New 4 Update J Delete J Show Deleted Records Exp No Animal Name Status Sex DOB Coat Ear Punch Cag Review By Owner
71. e Locations are already populated based on your default settings 4 Click Save 109 Recently Added Cages In left hand menu Cages gt Recently Added Cages you can view cages that were created in the last 24 hours Neurogenin 1 in brain tumor 29 Search TT Browse Settings g Print Cages Create Ca ge List of Cages Displaying 1 2 of 2 records Page 1 of 1 h Review Cages Revi pFAdd to Selection New Update 3 Delete J Export Cage Cards hk Review By Owner B Recently Added Cages 2 F No Cage Name Type Status Set Selected Cages 0 I 1 Cage 5 Active 2013 10 11 z we BEET IIT Pl 2 777777 Active 2013 10 10 a Cage Map H Animal Select an action Displaying 1 2 of 2 records Page 1 of 1 Measurements 110 Review Cages In left hand menu select Cages Neurogenin 1 in brain tumor p Search ey Browse j Settings Print gt Review Cages Cages 9 create Cage List of Cages Displaying 1 13 of 13 records Page 1 of 1 Review Cages Active 13 fAddto Selection New 4 Update Tj Delete export Jevent Cage Cards Breeding 0 F No Cage Name Type Status Setup Date MDA Cages Animals lal Holding 13 F cage 33 Active 2013 10 CDF 6 il Discarded Ef F7 cage 23 Active 2013 10 male 8 male 9 t Review By Owner 11 i Recently Added Cages 12 Fi cage 8 Active 2013 10 CDF 10 Selected Cages 0 11 cage d Active 2013 10 CDF 7 CDF 8 CDF 9 amp
72. e 7000000 Mating Details Q Dam 4 A Female 1 Cj ire V Male Mating Date 2013 10 12 Plug Date 144 4 Note that the existing dam has already been selected Do not uncheck A m E al Fl E E gt p I3 3 E al Fl E m No 10 11 LZ 13 14 15 Animal Name 1 2 CDF 7 CDF 8 CDF 9 CDF 10 Female 1 Female 2 Female 3 neuro 1 neuro 3 neuro 7 neuro 8 neuro 9 neurogenin 8 hs Status Sex Live Live Live Live Live Live Live Live Live Live Live Live Live Live Live 145 F DOB 2013 10 11 2013 10 11 2013 06 06 2013 08 06 2013 08 20 2013 10 11 2013 08 20 2013 08 21 2013 09 03 2013 10 12 2013 10 12 2013 10 12 2013 10 12 2013 10 12 2013 10 09 List of Animals Displaying 1 15 of 15 records Page 1 of 1 Owner Naomi Iwal Naomi Iwal Naomi Iwal Naomi Iwai Naomi Iwal Naomi Iwal Naomi Iwai Naomi Iwai Naomi Iwal Naomi Iwal Naomi Iwai Naomi Iwai Naomi Iwal Naomi Iwal Naomi Iwai Cage Neurogenin 56 Meurogenin 56 Meurogenin 56 cage a cage a 7000000 femi Cage 6 Cage 5 Cage 5 Cage 6 Cage 6 Cage 6 Cage Breer Breet Breer Breet Breet Breet Holdi Hold Breet Breer Holdi Holdu Holdii 5 Select the female s that you want to add to the mating 6 Click Select button 11 12 13 14 15 2 OF 7 CDF 8 CDF 9 CDF 10 Female 1 Fem
73. e Date M 2 Text Single Line e 22 Set Up Genes and Genotypes Set up all genes and genotypes that are used in the lab You can always return to edit or add more genes and genotypes 1 In left hand menu select Genes showing All Projects and Genotypes gt Create New Main Information Gene and Genotypes Cages Animals Measurements Genotypes e g KO To enter multiple genotypes please seperate Gene e g Pten ah Lab Events Matings Litters Review Gene and Genotypes Selected Gene and Genotypes 0 Search Gene and Genotypes Notes d IACUC Protocols d Billing Accounts b System Settings gt Users Help 23 Enter the gene name in Gene field Enter all possible genotypes in Main Information FGFB e g Pten Genotypes field in separate lines see right Genotypes e g KO Click Save To enter multiple genotypes please seperate each al j _j Edit Genes and Genotypes To edit a gene name or genotype or add more genotypes to a gene return to the Genes and Genotypes tool and select Update or Edit 24 Main Information Gene FGFR e g Pten Genotypes Check to remove the genotypes from this gene Genotypes Usages 3 is A New Genotypes To enter multiple genotypes please seperate each value by A Note if you edit a gene name or genotype the change will reflect on all existing records For example if you change
74. e cards will look like this If you select a Holding cage that contains more than 5 animals and click on Cage Card an error message will display and you cannot create a cage card If you select a Breeding cage that contains more than 2 dams and click on Cage Card an error message will display and you cannot create a cage card Cage Cage 2 Setup Date 2013 10 09 Owner Naomi Iwai Phone 713 798 1111 PI RISTS Project Neurogenin 1 in brain tumor Protocol 99 11 2346 Billing Account Matings neurogenin 1 BSRB SB 3000 1 A 6 Animal DOB Coat Ear Punch Genotypes 5 neurogenin 10 2013 10 09 Agouti 15 FGF8 Meurogenin 1 het Di neurogenin 8 2013 10 09 Agouti 11 FGF8 Neurogenin 1 het D2 Litter DOB neurogenin 1 2013 10 11 Error There are more than five animals in this cage Please verify your cage and try again Please click the following to review Cage A12 Error There are more than two dams in this cage Please verify your cage and try again Please click the following to review Cage 2 120 Cage Report You can create and print a report that displays cage and animal information 1 In Review Cages List select List of Cages Displaying 1 7 of 7 records Page 1 of 1 the cages for which you want to create a cage rAddto Selection New Update J Discard Cages Project Cage Cards Cage Report J Deh report by checking the check boxes No Cage Name Status Purpose Setup Date Animals Owner
75. e these ame Rack values Setup Date 2013 09 25 Notes Owner Naomi Iwai I MDA Cages Building TACUC Protocol brain tumor 29 888 777 Purpose Holding Room Row Column Cancel N A e g Animal is not available or not alive You can choose this option of not selecting a cage if your animal is not available in the colony or not alive Note that the animal Status field will 45 automatically change to Dead Main Information Heuragenin 1 in brain tuma _ Choose from all active cag Set up a new cage Owner About This Animal Animal Mame SEX Unknown Status Dead 46 4 Enter Animal Information and click Save Animal Name is mandatory remaining fields are all optional Note if you chose an auto naming rule for animal names in your Project settings animal name will be created accordingly Coat Strain Background Strain Source Purpose are all drop down menus which have been set up in Settings by the lab mLIMS manager Genes and Genotypes can be selected from a list set up in Genes and Genotypes by the lab mLIMS manager You can enter the dam and sire mother and father of the animal by selecting them from the magnifying glass icon list Click on Click here to attach files to attach a file to this animal record About This Animal Animal N SEX Unknown I Status ive
76. eate Cag Cl Auto Create To update and add Tagging information Change the status of the litter to Is Tagged Yes Update the Tagging Date Enter information in Pups table such as Animal Tag Numbers Sex and Coat color take advantage of the Auto Fill Auto Update and Copy functions Click Save Progress ls Active Date of Birth 2013 10 13 ls Genotyped Wo Genotyping Date ls Weaned No Weaning Date 2013 11 03 Animal Name Toe Clip Sex Set the first 3 animals to f Update The rest of the animals will be set to opposite Auto Fill F Agouti F Agouti F Anmut i 170 Genotyping To update and add Genotyping information Change the status of the litter to k Active Is Genotyped Yes ls Tagged Update the Genotyping Date ls Weaned Progress Date of Birth Tagging Date 171 2013 10 13 2013 10 23 2013 11 03 Enter information in Pups table Genes and Genotypes column take advantage of the Copy function Click Save Weaning Create Cage 9000000 9000000 93000000 93000000 Auto Create ag Genes and Genotypes Copy X4 Neurogenin 1 I het X FGFB I Add More X Neurogenin 1 B X FGFB Add More 3E Neurogenin 1 B X FGF8 Add More Ll 1 l X Heurogenin 1 B het 172 To update and add Weaning information Progress Change the status of the litter to Is Wea
77. ed Records E e Review Animals NO Animal Name Status Sex DOB Coat Ear Punch Review By Owner FE i testing pup 9 27 Live 2013 09 27 Black Review By Status 2 testing 9 27 2 Live 2013 09 27 Black P Review By Age F 3 Practice1 2 live 2013 06 26 z i Recently Added Animals 10 i tro 4 PracB 4 Live F 2013 06 27 3 Selected Animals 0 I 35 PracB 3 Live F 2013 06 27 2 amp Search Animals MNA LUDUM 6 PracB 2 Live F 2013 06 27 1 t Measurements MER URS UR UR RURAL E m 7 PracB 1 Live M 2013 06 27 Lab Events is eee hae ee Gia ee Ee eT e a Ema TUE TES a E TEE UR a Ua TE a P B Prac A 4 Live F 2013 06 27 Matings Litters perigee ud 10 neurogenin 9 Live M 2013 10 09 Projects ARIA ORA UR M UR A URS A S RS URP SP PA Pa P M BUR PP RB A A UP HORA PO Reo d ea li neurogenin a Live F 2013 10 09 Genes and Genotypes ET aR re ie hee mi Dtm opa qp temple pp LX IL aa a F7 12 neurogenin y Live F 2013 10 09 P IACUC Protocols ec i eS l ene ee ee 64 Shortcut buttons for Review Animals List List of Animals Displaying 1 10 of 10 records Page 1 of 1 Rec rAddto Selection New Update J Delete J Show Deleted Records z Export Event f sacrifice Move Mating Add to Selection see below The Animal Shopping Cart New Add New Animal button see above Add New Animal Update see below Update Animal Information Delete Show Deleted Records see below Delete Anim
78. enin 3c neurogenin 4g Building BSRE Room 56 8000 Main Information Project Neurogenin 1 in brain tumor I Cage Choose from all active cages Setup a new cage Mating Details Mating neurogenin 2 5 Follow steps 5 11 in above Create a Mating from Review Animals List Create a Mating from Matings gt Set Up Mating 138 1 Select in left hand menu Neurogenin 1 in brain tumor v TT Matings gt Set Up Mating Cages i ana Main Information Animals or See Soe ae oe eae ee ee eee oe ee ee See Project N P i eurogenin 1 in brain tumor Measurements E Lab Events Cage Choose from all active cages i Set up a new cage Matings gt Set Up Mating Active Cages Review Matings Mating Details H Review Mating By Status Selected Matings 0 Mating neurogenin 3 Em 4 Search Matings Q Dam 1 Haomi bwai Litters sire A Projects Transfer the selected animals to this breeding cage David Cotte j Cones and Lenotupes ang v Verify that there are na other animals living in this breeding cage Il Jane Dae punte ARR PEUT TU Eis emer E eR EU ME RNC UL E UI UR iu uc M iA Ui M uU M iM M c t E UE _ Naomi Test Billing Accounts Plug Date 5013 09 27 System Settings Is Pregnant Ma Nina Chang Pregnancy Date Viri Chakra Delivery Date 2013 10 15 Retire Date 2 Select a breeding cage Main Information IEEE You can set up a new cage or Main Inform
79. ent etc The Shopping Cart function is available for all other records such as Cages Matings Litters etc and the same instructions apply 1 In Review Animals List select the animals that you want to List of Animals Displaying 1 97 of 97 records Page 1 of 1 group together by checking new Update J Delete J Show Deleted Records glExport F jEvent 3 sacrifice Mo the check boxes 2 Click on Add to Selection NO animal Name Status Sex Genes amp Genotypes DOB Coat Ear Punch Cage 1 12 Live k 2013 83455 06 27 2 13 Live 2013 83455 06 27 14 Live 2013 7834535 06 27 Live 2013 0 PracB 6 06 27 Live F 2013 31 PracB 4 06 27 Live 2013 83455 06 27 Live abc 12a 2013 12 A 1 ma mad 68 3 Note that in left hand menu Showing All Projects 29 Search pul Browse ri Browse Deleted j Settings Print Selected Animals is now highlighted and you see the List of Animals Displaying 1 97 of 97 records Page 1 of 1 Animals number of animals Add New Animal Continue to add animals to add Multiple Animals Pe ieee bia cu dede cH Bele C Shaw ict teenie the shopping cart if B Review Animals Fl No Animal Name Status Sex Genes amp Genotypes applicable Review By Owner fF 1 12 Live M This feature is convenient when uisi CE EN es E TOIT ou have to go through a long list Review By Age P of records or through different Selected Animals 2 pages to select your desired Search
80. etire Date and you can select the email recipients Mating Q Dam x C Sire Mating Date Plug Date Is Pregnant Pregnancy Date Delivery Date Retre Date Status Email Hotifications Notes neurogenin 3 p Ox e OX Transfer the selected animals to this breeding cage J Verify that there are no other animals living in this breeding cage 2013 09 26 2013 09 27 No I 2013 10 16 Active Yes 196 Email Not Naomi hai David Cotten Naomi Test Hina Chang Viji Chakravarthy On the day of the scheduled From mLIMS lt do not eply mlims mdanderson org gt mating delivery or retirement at 7 12am selected recipients will cc receive an email to their MDA subject mLIMS Mating Delivery Reminder Test mating 6 26 2 2013 08 30 email address Iwai Naomi More Details The email subject line will indicate the type of reminder Mating Main Information Mating Delivery or Mating Retire Mating Test mating 8 28 2 and the Mating Name and date of Mating Date 2013 08 28 scheduled event Plug Date 2013 08 28 A Is Pregnant N The email content will display all Nini information related to this mating Delivery Date 2013 08 30 including links that direct you to the mi imal i LIMS Retire Date mating or animal in m l air Acie Notes Additional Information Strain Background Strain Category Diet Generation Expected Number of Pups
81. ge Locations Cage 6 Female 3 Building BSRB Room 56 8000 fem1 Female 2 Building BSRB Room 56 8000 7000000 Female 1 Building BSRB Room 58 8000 Sires Cage Animals Cage Locations 7000000 Male Building BSRB Room 56 8000 148 11 Move the females to the List of Animals Displaying 1 22 of 22 records Page 1 of 1 new Update Delete J Show Deleted Records 2 sacrifice ouo 9n breeding cage using the Move Animal button _FAdd to Selection 149 NO Animal Name Status Sex DOB Owner Cage Cage Purpose Genes amp Gi m i CDF 7 Live F 2013 Naomi Meurogenin Breeding FGFS N 08 06 Iwal 56 1 wt 2 CDF 8 Live F 2013 Naomi FGFS 08 06 Iwai Neurogenin 3 CDF 9 Live F 2013 Naomi cagea Breeding FGFS 08 20 Iwal Neurogenin 4 CDF 10 Live F 2013 Naomi cagea Breeding 10 11 Iwai 5 Female 1 Live F 2013 Naomi 7000000 Breeding Neurogenin 08 20 Iwal 6 Female 2 Live F 2013 Naomi femi Holding Neurogenin 08 21 Iwal 7 Female 3 Live F 2013 Naomi Cage 6 Holding Neurogenin 09 03 Iwal g Male Live M 2013 Naomi 7000000 Breeding 08 15 Iwal g male 7 Live M 2013 Naomi cagea Breeding 10 1 1 Ti AI tae Female 7 Female 3 Main Information The system will take the protocol information from the target cage and apply the animals 8 8 Choose from all active cages 7000000 Breeding M 1 F 1 J Set up a new cage 12 Click on Cage link to review cage information
82. genin 1 DOD FGF8 95 4 Set up how you want to sort the list Field Sorting Options 5 Click on Apply Changes Sort By Animal Name Ascending Add More 96 6 You should see the List of Animals with your preferred columns and column order Search qj Browse 3 Browse Deleted 4 Settings Print List of Animals Displaying 1 10 of 10 records Page 1 of 1 Rec Add to Selection New Update Delete J Show Deleted Records Export Event bf sacrifice move Mating This column view will be saved even after you log Out No Animal Name Status Sex DOB Coat Ear Punch Cage Cage Purpose Neurogenin 1 FGFS8 neuragenin 1 Live M 2013 10 09 Cage 1 Holding het neuragenin 2 Live 2013 10 09 Cage 1 Holding null f neurogenin Live 2013 10 09 Cage 1 Holding null 3 neuragenin 4 Live 2013 10 09 Cage 1 Holding het neurogenin 5 Live 2013 10 09 Cage 1 Holding het neurogenin 6 Live 2013 10 09 Cage 27 Holding het neurogenin 7 Live 2013 10 09 Cage 2 Holding wt neurogenin 8 Live 2013 10 09 Cage 2 Holding M M M F F F F M neuragenin 9 Live 2013 10 09 Cage 2 Holding Search Records and Save Searches The following instructions apply to searches in all Lists such as Review Cages List Review Matings List Review Litters List etc 97 1 Select Project Neurogenin 1 in brain tumor J Search qj Browse qj Browse Deleted 7 Settings MT Prin Select the Project in w
83. genin 1 in brain tumor Animals 9 Add New Animal Add Multiple Animals H Review By Owner Review By Status H Review By Age Recently Added Animals 35 Selected Animals 0 4 Search Animals Saved Searches v o St v rx IGI US RID ORIGINE URGIUGIORTR CRDI Matings va Search Browse WW Browse Deleted Settings 2 i z s List of Animals Displaying 1 48 of 48 records Page 1 of 1 Add to Selection 205 1 Boi CDF 7 CDF 8 CDF 9 CDF 10 Female 1 ONew 34 Upd ate 3 sacrifice Live Live Live Live Live nra F DOB 2013 Da 14 2013 D amp 06 2013 Da 06 2013 08 20 2013 i0 11 26013 Owner Naomi Iwai Naomi Iwai Naomi Iwal Naomi Iwat Naomi Iw al Naomi Mating Cage 9000000 cage 232 AZ cage a cage a Print Cage Purpose Genes amp i Breeding Halding Halding Breeding Breeding 7nnnnnn Areaeadina FGFS Neurogen FGFS 4 Neurogen FGFS 4 Neurogen MogrErnaon checking unchecking the Main Information check boxes Selected Cages Animals No Cages Animals Locations V cage a cor 9 E cor 10 El male 7 Building BSRB Room 58 800 W cage 232 Ml CDF 7 Building BSRB Room 58 8001 I A2 W CDF 8 Building BSRB Room 58 800 Event Details Project Neurogenin 1 in brain tumor I Title Start Date 2013 10 13 End Date 3013 17 12 206
84. heck boxes NO Animal Name Sex DOB Cage Background Strain Genes amp Genotypes Status Age Cage Pur In this example am moving 3 Swiss 1 F 2013 Live Od animals Swiss 1 2 and 3 rag Swiss 2 3 2013 gm Uu 09 26 Swiss 3 F 2013 Live Od 09 26 Swiss 4 Live 09 26 Swiss 5 F Live 09 20 Swiss 6 F Live 56 You can choose where to move Animals To Be i the animals to an existing cage 3 or to a new cage Swiss 1 Swiss 2 Swiss 3 Main Information The system will take the protocol information from the target cage and apply the animals Cage Choose from all active cages 7 3 Set Up anew cage If you select Choose from all t Pana f i B Choose from all active cages active cages you will see all Choose from all active cages cages in this Project Select one LJ Set up a new cage and click Save the animals will en ae g 5 aldiri nj F be moved to this cage re ae blag a sake 5 777335 Breeding M 0 F 0 ZT7400 Holding M 0 F 0 7 1 Reset 777401 Holding M 0 F 0 Cage 1 Holding M 0 F 5 Cage 2 Holding M 0 F 5 57 Or you can select Set up a new cage and the Create Cage window will display Enter cage information and click Save Main Information Project Neurogenin 1 in brain tumor Owner Naomi Iwai About This Cage Cage Name MDA Cages IACUC Protocol brain tumor 99 888 777 Biling Account Purpo
85. hich you Cages want to search eee RORODORODODOE List of Animals Displaying 1 10 of 10 records Page 1 of 1 Animals 9 Add New Animal i j Oo Lr f d 9 Add Multiple Animals jT Add to Selection New 4 Update Tj Delete J Show Deleted R E Review Animals l NO Animal Name Status Sex DOB Coat Ear Review By Owner neurogenin 1 Live M 2013 10 09 Review By Status neurogenin 2 Live 2013 10 09 Review By Age Recently Added Animals 10 Selected Animals 0 neuragenin 3 Live 2013 10 09 neurogenin 4 Live 2013 10 09 ji neurogenin 5 Live 2013 10 09 Search Animals Measurements neurogenin 7 Live 2013 10 09 neurogenin 8 Live 2013 10 08 1 2 3 5 o 5 g Matings neurogenin 9 Live 2013 10 09 i i i F neurogenin 6 Live F 2013 10 09 F ae m Litters OVE ket aera renee ck erent M mua irnnaanm d nra 113 1n oma ak a You can also select Showing All Projects and the search will apply to all Projects 98 Showing All Projects l E Search wil Browse ra Browse Deleted 4 Settings Print Cages Animals 9 Add New Animal m A o T Talant a LT 9 Add Multiple Animals T Add to Selection New Update J Delete J Show Deleted Records Review By Owner al 1 2 Live 2013 06 27 Review By Status 13 Live 2013 06 27 Review By Age Recently Added Animals 10 Selected Animals 0 14 Live 2013 06 27 30 Live 2013 06 27 Jl Live 2013 06 27
86. imal names are unique If by M OF 1 Selected Animals 0 265 you selected the Naming Rule p all animals have the same 6 3 F 2013 Cage l Measurements 09 1 name all animals would have 26 been named B6 Note that new cages have been created automatically Cage 1 Genes and Ge and Cage 2 and that animals IACUC have been distributed in the Billing Accounts cages You can redistribute the System Settings animals however you like by using the Move Animal function see below 10 B6 10 If in above step 3 you chose to not automatically create cages 54 Leave check box Automatically Main Information Create Cages unchecked Project Neurogenin 1 in brain tumor Automatically Create Cages Uncheck this option if you prefer to assign the z Ho of Animals 40 Animal Mame Initial Swiss Animal Information SEX Female Date of Birth 9973 09 24 Note that the newly created animals are not in cages uncaged animals No Animal Name Sex DOB Cage Background Strai 1 Swiss 1 F 2013 99 26 Swiss 2 2013 09 26 Swiss 3 2013 pa 26 Swiss 4 Swiss 5 Swiss 6 Swiss 7 Swiss 8 Swiss 9 55 You can use the Move button to List of Animals Displaying 1 10 of 10 records Page 1 of 1 move the animals to a cage first select the animals you want to move by checking the y rAddto Selection New Update J Delete Export Event S Sacrifice Move Mating c
87. imals Rover EY ae Animal Name Initial Review By Status Naming Rule F All animals have the same name For Review By Age Recentlv Added Animals 3 Ammal Infarerestiarn 49 3 Choose if you want the system to automatically create cages or if you want to create cages later If you want the system to create cages check the check box Automatically Create Cages The system will create cages and distribute your animals in these cages If you want to later assign them in cages leave the check box unchecked In this example we will automatically create cages 4 Checkthe check box 5 Note that IACUC Protocol and Billing Account fields appear The cages will have this protocol and billing account You can change these by selecting a different protocol or account from the drop down menu Note you will not see the Billing Account field if no billing account has been created Main Information Sg Pree t Automatically Create Cages Ho of Animals Animal Name Initial Main Information Project Automatically Create Cane J amp CUC Protocol Billing Account Ho of Animals Neurogenin 1 in brain tumor Uncheck this opton if you prefer to assign the animals to your own cages Neurogenin 1 in brain tumor EB brain tumor 123 444444 50 6 Enter No of Animals and Animal Name Initial Select Naming Rule if you check this check box all animals will h
88. ip Copy Auto Fill neu 76 neu 78 6 Fill in any other information you have e g Sex Coat etc 166 we Initial peu Toe Clip 76 ew Born 5 i Select if you want to create a litter record without pups You can add more pups when editing this litter record Animal Mame 5tatus Toe Clip Sex Coat Cage Genes and Genotypes Notes Set the first 1 animals to Updat Create Cage Copy Auto Fill Tp gt d i ES Copy Copy Copy The rest of the animals will be set to opposite Auto Create Cages ice Th Live 7 39000000 I Xx m Add More iei T7 Live Im 7 9000000 I X I Add More idi TE Live l 78 f l 9000000 el 3 fal 7 Click Save and the litter and pups are saved Again you don t have to enter pup number and information You can create a litter only and later add pups 167 Update Litter Tagging Genotyping Weaning At any time you can update litter and pup information including tagging genotyping and weaning information In Litters Review Litters select the litter you want to update by checking the check box 2 Click on Update You can also click on the Litter Name and click Edit Animals H Review Litters P Review Litter By Status z Selected Litters 0 Search Litters b Neurogenin 1 in brain tumor Cages Litters Add Litter Projects Measurements Jo Search quy Browse j Settings Print List of Litters Displaying 1
89. l Abbreviation Protocol Number Total Animals Quota No Litters mE W 1 Study of brain brain tumor 11 222 333 100 sonra I m tumors and the Projects effect of surgery in genetically Genes and Genotypes modified mice IACUC Protocols E Drug injection Drug injection 22 444 777 o study to study the mlifjr de iiid effect of chemicals Review Protocols on reproductive behavior Selected Protocols 0 amp Search Protocols Select an action Displaying 1 2 of 2 records Page 1 of 1 d y d d 7 d 218 2 Click on the Renew showing All Projects 29 Search aj Browse mi Browse Deleted y Settings Print Cages Animals Measuremen ONE UU JF Add to Selection New Gf Update LJ Delete Show Deleted Records Export Lab Events Matings Litters 1 Study of brain brain tumor 11 222 333 100 svtneeneeeeneveenteneneentneneenentnenneettntentnesntneentntntntenretantetnteetnesete tumors and the effect of surgery in rd genetically 2 ee IR modified mice IACUC Protocols Drug injection Drug injection 222 444 777 9 create New Protocol study to study the effect of chemicals Review Protocols on reproductive behavior Selected Protocols 0 C Search Protocols Select an action Displaying 1 2 of 2 records Page 1 of 1 n butto List of IACUC Protocols Displaying 1 2 of 2 records Page 1 of 1 ow ir wm No rACUC Protocol 7 Protocol Abbreviation Protocol Number Tota
90. l Animals Quota No Ld d H r 219 3 Enter inf ti TOP anke Renew Protocol Study of brain tumors and the effect of surgery in genetically modified mice protocol and click Save New animals will be created with the new protocol number All existing live animals with the old protocol number will be changed to the new protocol number All existing dead animals with the old protocol number will remain under the old protocol number IACUC Protocol Title Study of brain tumors and the effect of surgery in ger Protocol Abbreviation brain tumor Protocol Number 11 222 333 Approval Date 2013 09 24 Expiration Date Total Animals Quota Notes Click here to attach files Note When you click Save all existing live animals under the old protocol will be changed to the new protocol All existing dead animals will remain under the old protocol 220 4 You will see a message saved Click on Please click here to review your f The data has been saved record Please click here to review your record 221 5 System will display information about your renewed protocol Note that you can view all renewal history in the IACUC Protocol Histories tab Summary LACUC Protocol Histories 1 Audit Trails 5 Main Information IACUC Protocol Protocol Abbreviation Protocol Humber Owner Total Animals Quota Approval Date Expiration Date Notes of Live Animals
91. lected Cages 0 y Search Cages amp Cage Map Animals Measurements Lab Events Litters Projects Genes and Genotypes IACUC Protocols Billing Accounts System Settings Main Information Main Information Project Neurogenin 1 in brain tumor I Owner Naomi Iwai m About This Cage Cage Name MDA Cages IACUC Protocol brain tumor 99 11 2346 Billing Account 11 22222 I Purpose Holding v Type Status Active Setup Date 2013 10 11 Hates Click here to attach files 108 Building Room Rack Row Column Locations BSRB 58 8000 2 Create Cage window Main Information displays Project Weurogenin 1 in brain tumor Enter all mandatory Owner Naomi Iwai I information such as Cage Name IACUC Protocol About This Cage Purpose of cage and non AEE lac mandatory information if MDA Cages i Building BSRB applicable IACUC Protocol brain tumor 99 11 2346 I Room SB B 000 Bill A count 44_9 A cage name is mandatory and iling Account 41 27929 v has to be unique in the lab Purpose Holding m Rack there cannot be two identical Type cage names in the same lab T M Status Active B Column setup Date 2013 10 11 For Smithville users Billing Notes Account may be mandatory too depending on your setup Note that some information such CBck here to attach files as IACUC Protocol and Cag
92. male 3 Holding 2013 10 pup pup 13 DLUD DUD BrdU injection New 2013 10 30 Naomi Iwal 7 000000 Active Naomi Iwal Active HOME Hello Naomi Iwa i LOGOUT Admin My calendar My Animals My Cages Environment Neurogenin 1 in brain tumor 29 Search ipu Browse j Settings 7 Print Cages Sonn ea P TEE List of Lab Events Displaying 1 2 of 2 records Page 1 of 1 t Animals Measurements as a a ne aR Ve RSI UTITUR TRITT rAdd to Selection ONew Update fy Delete Export Lab Events 9 New Event I No Review Events By Researcher Title Status Animals Cag Male Female 1 Female 2 Cage Female 3 neuro 7 neuro 8 700000 neuro 8 CDF 7 CDF 8 CDF 9 Brdu New injection Review Events By Status F cdf New Selected Events 0 ti Search Events Select an action Displaying 1 2 of 2 records Page 1 of 1 b Matinoc 212 Your Events will be displayed in a calendar view Note Only active Events will be displayed i e Status is New Proposed or In Progress Completed and Canceled Events will not display on the Event Over Due calendar Saco Overdue Events display in red 213 October 2013 Wed Event Normal BrdU 3 days Event Normal Brdu injection When you create an event mLIMS mLIMS lt do not eply mlims mdanderson org gt sends an email to the selected To Iwai Naomi recipients immediately to notify them of the event creation Subject mL
93. nd Genotypes tool Access To Protocol Management User has access to the IACUC Protocols tool Access To Billing Account Management User has access to the Billing Accounts tool Note to Houston users If you don t need to manage billing accounts uncheck Access To Billing Account Management so that Billing Account fields do not display in the system Set Up Settings For Your Lab Before entering data into mLIMS it is important to set up settings for the lab It is highly recommended that permission to Settings is restricted to the lab mLIMS manager on how to manage user permissions see above Manage User Permissions The lab mLIMS manager should be responsible for setting up the lab settings and making changes if necessary 1 In left hand menu select System Showing 4 Prajacts Settings Settings SEES WIN E GEE E Measurements Hame Fomai rut Hamal Last Hamal inm ishn Smith Lab Evoants Currency Sonbiol 5 Matings Date Farmat Yaar H digits om DESI T Honth i drii with lmnadinsg zaras a a ei Litters Dw Code with hadre mre gx Cl i Projects Tite Exmapis bie 3 POT Gents ind Comey pes LALIC Pr bocnis 7 m i eur a Lens Pane oon im l urz nl valer wa gl GROOT Laos ail Shiels b Leser Help 10 2 Main Information tab Select your preferred time zone date format etc 3 Cages tab Main Information Cage Capacity set to 5 mLIMS will not restrict the number of animals per cage Is Cage Req
94. ned Yes ls Active Yas Date of Birth ls Tagged Yes Tagging Date Update the Weaning Date ls Genotyped Yer Genotyping Date 173 2013 10 13 2013 10 23 2013 10 28 To wean you have three options in the Cage column of Pups table Option 1 Auto Create Cages the system will automatically create weaning cages and distribute females and males Option 2 Create Cage you will create the weaning cages and distribute females and males Option 3 Choose from existing cages in drop down list and distribute females and males Create Cage Auto Create Cages 9000000 9000000 9000000 9000000 9000000 ml 174 Genes and Genot Copy FGFB I ES Neurogenin 1 I Add More X FGFE I 2 Heuragenin 1 B Add More X FGF8 I A Neurogenin 1 B Add More FGFB I E Heurogenin 1 BE Add More X rra e Thai Option 1 Auto Create Cages L Cage Genes and Genc Check the check box Auto Create Cages and click Save PY Auto Create Cages Copy 9000000 X rara E A Neurogenin 1 E Add More 3000000 X rara E E Neurogenin 1 E Add More 3000000 X rara E P Heurogenin 1 E Add More annnnnn Click on Please click here to review your record 175 Review your litter in Pups tab confirm that females and males have been distributed correctly to weaning cages The data has been saved Please click here to review your record Litter
95. nformation The system will take the protocol information from the target cage and apply the Cage amp Choose from all active Cages Set up a new cage Cage 2 Holding M 2 F 3 JTTITT7 Holding M 0 F 0 Cage 5 Holding M 0 F 0 Project B Main Information t Cages Animals 2 Animals To Be Moved 9 Add New Animal Uu TT neurogenin 6 neurogenin 7 9 add Multiple Animals i Review Animals M Main Information Review By Owner Review By Status The system will take the protocol information from the target cage and apply the animals Review By Age biaa Selected Animals 4 A Choose from all active cages et up a new ca amp Search Animals Tm Demo B 3 Breeding M 1 F 0 hk Saved Searches Demo B 4 for females Holding M 0 F 3 Demo B 5 for males Holding M 1 F 0 i See 21a Breeding M 1 F 1 Lab Events Scenarios for Move Animal to Different Project e Ifyou want to set up a mating between animals that belong to different Projects you have to use this Move Animal function first to make sure that both breeders are in the same Project If you want to give an animal to another lab member you can use this Move Animal function to move your animal to your lab member s Project Note that your lab member will not be able to use this animal if he she does not have update permission to this animal Example Naomi moved her animal neurogenin 9 to Viji s Project However Viji cannot do an
96. ng the check boxes No Cage Name ms Purpose tetup Animals Owner IACUC Protoco 2 Click on Cage Cards ly 1 Neurogenin Activa Holding T013 09 CDF5 Naomi brain tumor 99 56 4 Iwal 11 2546 caged tiva Holding PT CDF 7 CDF 8 CDF 9 Naomi brain tumor 99 Iwal 11 2346 Cage A12 Activa Holding T male 107 Naomi braim tumor 99 l Iwal 11 2346 cage a Active Breeding 2013 10 CDF 10 male 7 Naomi brain tumor 99 11 Iwal 11 2346 Cage 2 Active Breeding 2013 10 neurogenin 8 Naomi brain tumor 99 na narrnnmanim 10 1 7 Taal at 119344 118 3 Cage cards will be Cage Neurogenin 56 Setup Date 2013 09 14 displayed these are for Owner Naomi Iwai Phone 713 798 1111 PI RISTS Project Neurogenin 1 in brai Holding Cages Protocol 99 11 2346 Billing Account 11 22222 BSRB SB 8000 1 B 4 Animal Sex DOB Coat Ear Punch CDF 5 M 2013 09 17 Black 12 Cage cage d Setup Date 2013 09 14 Owner Naomi Iwai Phone 713 798 1111 PL RISTS Project Neurogenin 1 in brai Protocol 99 11 2346 Billing Account 11 22222 BSRB SB 8000 1 A 4 Animal Sex DOB Coat Ear Punch Genotypes CDF 7 F 2015 08 06 Agouti 40 FGF8 r Meurogenin 1 wt CDF 8 F 2013 08 06 Agouti 41 FGF8 Neurogenin 1 het CDF 9 F 2013 08 20 Black 42 FGFS Neurogenin 1 wt Cage Cage A12 Setup Date 27013 09 14 Owner Naomi Iwai Phone 713 798 1111 PIRISTS Project Meurogenin 1 in brai Protocol 99 11 2346 Billing Account 11 22222 119 Breeding cag
97. null null 3 neurogenin 3 M 2013 10 09 Neurogenin 1 null null 4 neurogenin 4 M 2013 10 09 Neurogenin 1 het het 5 neurogenin 5 M 2013 10 09 Neurogenin 1 het het 6 neuragenin 6 M 2013 10 09 Neurogenin 1 het het 7 neurogenin 7 M 2013 10 09 Meurogenin 1 wt wt You will have the option to display gene name as column header and genotype as value see above red box in addition to the default display of genes and genotypes see above blue box For how to set up which columns you want to see and in which order see Section 5 Animals Set Up Column View 40 12 Users Tab Main Information Record Naming Cage Locations Mating amp Litter Genes Order You can give other users in your lab Users Can Update Records edit access to your Project by checking the check box under Can Naomi Iwai Project Owner Update Records David Cotten In this example Naomi can update records in this Project because she is the Project Owner Debra Montague Has permission to update any records l Jane Doe Debra can also update records in this Project because she has Update Other Researchers Records permission in Users Permissions see Section 2 Manage User Permissions Mami Hei But if you want additional users to be able to update records in this Project check the check box for the users In this example Jane Doe has been given edit access To disable edit access simply uncheck the check b
98. of Dead Animals of Total Animals of Active Cages Study of brain tumors and the effect of surgery in genetically modified mice brain tumor 99 BBB 77 Naomi Iwai 0 2013 09 24 2016 09 24 222
99. om the active database Please think carefully before proceeding neijurnaenin amp I we FGFAR Neuronenin 1 List of Animals Displaying 1 8 of 8 records Page 1 of 1 vF Add to Selection Hew 34 Update ul Delete ul Show Deleted Records neurogenin 3 neurogenin 4 neurogenin 5 neurogenin 6 neurogenin 7 neurogenin amp neurogenin 9 m E m m E E E E E neurogenin 10 Live Live Live Live Live Live Live Live No Animal Name Status Sex M 72 Genes amp Genotypes FGFS Neurogenin 1 null FGFS Neurogenin 1 het FGFS Neurogenin 1 het FGFS Neurogenin 1 het FGFS Neurogenin 1 wt FGFS Neurogenin 1 het FGFS Neurogenin 1 het FGFS Meurogenin 1 2013 10 mating 3 Sacrifice DOB Coat Ear P 20 1 qJ 10 ga 2013 10 09 20 1 d 10 gae 2013 10 09 2013 10 09 2013 10 09 2013 10 09 2013 10 To view the deleted records click on Show Deleted Records List of Animals Displaying 1 8 of 8 records Page 1 of 1 3f Update 1d Delete CL Show Deleted Records Mating 2 sacrifice new rAdd to Selection A list of Deleted animals will display You can recover animals by clicking on the Undelete button Animal Mame neurogenin 3 neurogenin 4 neurogenin 5 neurogenin amp neurogenin 7 neurogenin amp neurogenin 9 neurogenin 10 rs Status Sex Live
100. omeone else set up email reminders and share the status of the event with others There are several possible ways to create an event 203 Method 1 amp banum Ts eee UA Cages In left hand menu select Lab x a Aniniale Measurements Lab Events Event Details Project Neurogenin 1 in brain tumor i E Review Events Title Selected Events 0 um W Naomi hw B RISO EOS Start Date 2013 10 13 Search Events E El Calendar View End Date 2013 10 13 Debra Mc Assi d Ta c E Nami Hei Matings X 9 Naomi Iwai y a Se a ae ap E E A EN Category EE F EE Projects 000 be a 7 Systema Genes and Genotypes ent ona i Fl dina us E A A ea ar E TERM System Settings Users Main Information Select animals for the event from the magnifying glass Anitak icon list e Animal A Event Details Note you do not have to Project Neurogenin 1 in brain tumor E select animals for an event Neurogenin 1 in brain tumor you can create animal less Title events Start Date 2013 10 13 End Date 2013 10 12 Assigned To Naomi Iwai Category v 204 Method 2 In Review Animals List select the animals for the event and click on Event In New Event window the selected animals and cages display Note that all animals in the cages are displayed even the animals that you did not select Select the animals for this event by Neuro
101. omi lwa LOGORTT Showing All Projects unn Abeer Maciraments Full Create Accam Racor Lab Events Pain Litters Projects Genes aud perp IACLIC Protecols wsbem Selinas b k i Shirred ie Persone CTU a a uen I Lil Laws alic nima Li esr Help in Grom Dean An My Calenda My Auenals My Cages Environment Test Ir amp raduczmg Upetate Hha Pars Valen Are Pur t Record doen Tg ores T Elling kiin Ey ie nei Anes To aoe T Sen Les Bent hha reel ones To Gee ud Can re bee Mw d ee rn hit Pio col Nondaqemenia Mere 2 For each user decide which Users Permissions DD permission level to give and check the respective check Pee OPE HN Update Other Access To Access To Access To Genes Access To Access To box e s i Pi n Dan aie Researchers System User and Genotypes Protocol Billing Account I Me Records Settings Management Management Management Management nu I iua an a I Introduce Default Settings You can return at any time to this table and change permission levels System Manager by checking unchecking check boxes viji Chakravarthy Debra Montague Naomi lwal Jane Doe 3 Click Save Description of user permission levels Full Access If you check this check box user will have full access to the system Create Records User can create records Introduce New Values to Drop Down Menu User can not only select from existing opti
102. ons in a drop down list but also type in new values In below example if you have permission to introduce new values you can type a value that does not exist in the drop down list e g FVB If you don t have this permission you can only choose from the pre set values in the list these values are set up in Settings by the lab mLIMS manager Background Strain FYB m Source p mixed Purpose pose Black 6 nes and Genotypes 1379 For most users Create Records and Introduce New Values to Drop Down Menu should be sufficient It is highly recommended that you restrict the following permission levels to the lab mLIMS manager Update Other Researchers Records User can make updates to all other lab members data Note in mLIMS all users have read only access to all data in the lab In other words you can see everyone s data and everyone can see your data However update edit access to others data is restricted and is controlled here in Users Permission table To grant a user update edit access to all records in the lab give this user Update Other Researchers Records permission You can also grant a user update edit access to a specific Project see Section 4 Setting Up Your Projects for more details Access To System Settings User has access to Settings Access To User Management User has access to the Users Permissions table and to the Users tool Access To Genes and Genotypes Management User has access to the Genes a
103. owing instructionsexplain a trio mating scenario but the same instructions apply to harem matings Set up a trio mating see above Set Up Mating select two females in a mating Main Information Selected Cages Animals You can also add a female to an existing one male vs one female Please select the animals to be included in this mating mating see above Add Female To Existing Mating m E nimals ocations Cage Al 7 male 13g Building BSRB Room 5B 800C Cage 6 J female 7 9 W female 8 9 Building BSRE Room 5B 800C Main Information Project Meurogenin 1in brain tumor x dtc Choose from all active In the example above let s assume female 7 is pregnant and you want to move her to a separate cage 1 Move pregnant female female 7 to a separate cage using the Move Animal function 186 see Section 5 Animals Move Animals Review Animal Information of Animal Edit Create amp Search female 7 summary Cage Matings 1 Animals In Cage 1 Lab Events 1 Cage History 3 female 7 is now alone in a separate Breeding cage you may want to mark Cage Type as Nursing Animal Hame Status Sex DOB 2013 female 7 we F 10 70000 Breeding Nursing 1 Click on Matings tab summary Matings 1 Animals In Cage 1 Lab Events 1 Cage History 3 Note that female 7 is still part of the mating although she is ina SIME ren cage Keep her in this Ho Status Cage Mating Date
104. ox 41 Main Information Record Naming Cage Locations Mating amp Litter Genes Order Users Can Update Records Naomi lwai Project Owner David Cotten Debra Montague Has permission to update any records Jane Doe w C Mami Hei Section 5 Animals 42 Add New Animal t In left hand menu select newogenin 1 n teain tumor e EMEN Animals gt Add New Animal cages Main Information Animals Ii Add New Animal Project weurogenin 1 in brain tumor l 9 Add Multiple Animals Cage E E E ae M z Review Animals set up a new cake bh Review By Owner J N A e g Animal is not available or not alive Review By Status Owner snd para I Naomi Iwai Review By Age F Recently Added Animals 2 About This Animal F Selected Animals 0 animal Mame Identifications Search Animals Measurements Ear Tag rens EN Status ius T Lab Events Bom lc i Date of Birth 2013 09 25 Matings T Coat vi Litters i Strain w Projects adn eye Backeround Strain Y Genes and Genotypes Source Wr t IACUC Protocols ee ERLE EES cde e arn up mue Le t Purpose x Billing Accounts m Genes and Genotypes Add More System Settings rm Q Dam 4 Help Cf sire Notes a i Click here to attach files Save Reset 43 2 Select Project you can select in either left hand menu Projects selection list or main Cages N information window Project Animals
105. rd Naming Cage Locations Animal Disable Auto Naming Animal Tag 9 Based On Abbreviation neu Abbreviation Animal Tag Pups Animal Name Initial peu Toe Clip Mo of New Born g I Select if you want to cre 163 leave the animal tag numbers blank and later fill in at the time of tagging No Animal Name Status Toe Clip Actions Copy Auto Fill The t 1 neu 1 Live 2 Live 2 3 Live 2 4 neu 4 Live 164 If you chose Abbreviation Animal Tag for Animal Names Animal Name Initial field will be populated with the Abbreviation you chose in Project you can change the initial for this litter by entering a different initial Enter the start number of animal tag optional Upon entering the number of newborns The system will create a table with the number of pups you selected It will name the pups based on the animal name initial and animal tag number you can leave the animal tag numbers blank and later fill in at the time Main Information Record Haming Cage Locations Matir Animal Disable Auto Maming Animal Tag Based On Abbrewviation 9 Abbreviation Animal Tag neu Pups Animal Name Initial peu Toe Clip Mo of New Born g Select if you want to cre 165 of tagging Pups Animal Name Initial pey Toe Clip 76 Mo of New Born 5 Select 0 if you want to create a litter record without pups Yo No Animal Name Status Toe Cl
106. re populated 157 4 Set up Email Notifications About This Litter Litter neurogenin 5 Y d mLIMS will send you email rog ifi j Email Notfications wa notifications for DOB reminders Yes o m ste ct trae AR Tagging reminders Genotyping Notes QO ome ane Weaning ls Tagged No Tagging Date 2013 1 reminders ls Genotyped Wo Genotyping Date if you don t want emails for this litter select Email Notifications No Is Weaned Wo Weaning Date 2013 1 Email Notifications In the Email Notifications box Naomi lwai David Cotten select the email recipients You can choose any of your lab members Nami Hei Naomi Test Debra Montague Jane Doe Hatalie Chang Hina Chang For more information on email MEET ORE te eet E notifications see below Mating amp Litter Email Notifications lisieiinip trace 5 Enter Animal Name Initial Animal Tag and Number of Pups Pups Animal Name Initial Toe Clip Mo of New Born g I Select Oif you want to create a litter re 158 Note You don t have to enter this information now You can create a litter without pups click Save without enterin information and later How your pups are named will depend on the settings in your Project see Section 4 Setting Up Your Projects Record Naming If you chose Disable Auto Naming for Animal Names Main Information Record Naming Cage Locations Mating Animal Disable Auto Naming Animal
107. rmation see below Event Email Notifications if you don t want emails for this Event select Email Notifications No In the Email Notifications box select the email recipients You can choose any of your lab members Status Proposed Completed Canceled Event Details Project Title Start Date End Date Assigned To Category Status Priority Email Notifications Motes Meurogenin 1 in brain tumor Brdl injection 2013 10 30 2013 10 30 Naomi Iwai W New Normal I 209 jm 6 Click on Save Email Notifications Naomi hai Debra Montague Nami Hei Natalie Chang System Manager David Cotten Naomi Test Nina Chang Viji Chakravarthy 210 Review Events There are several possible ways to review your events Method 1 In Lab Events gt Review Events review your events Method 2 In Review Animals List under Event Summary the Event Title Event Status and Event Start Date End Date are displayed for each animal Lab Events Neurogenin 1 in brain tumor J9 Search weal Browse j Settings g Print t Cages Animals List of Lab Events Displaying 1 2 of 2 records Page 1 of 1 Measurements P JF Add to Selection New Update 7j Delete Export New Event rj No Title Status Animals Cages Start Date Review Events 1 Brdu Mew Male Female 1 Female 2 Cage 6 2013 10 30 injection Female 3 neuro
108. s 0 Mating neurogenin 3 Em 4 Search Matings j Q Dam Ros Naomi lwai Litters Qj Sire Q eue rcp Mer tere CP MOL are hear ace cree WE ee a Ese en David ere Genes and Lenotupes j E v Verify that there are no other animals living in this breeding cage Fl Jane Doe pu UE IE PUE ETE T eater arene Eis emer uis Menem Peu pu pM nen ie Eh Pina BSEC ae ups iran paso aaa era aaa emere TN E FI Mina Chang E E a mack Naomi Test Delivery Date 2013 10 15 Retire Date Create a Mating from Review Animals List 129 1 Select in left hand menu Neurogenin 1 in brain tumor Search inj Browse E Browse Deleted 7j Settings Print Animals gt Review Animals List of Animals Displaying 1 15 of 15 records Page 1 of 1 9 Add New Animal Add Multiple Animals Add to Selection New Update J Delete J Show Deleted Records Export Event 2 Sacrifice NO Animal Name Sex DOB Cage Background Strain Genes amp Genotypes Status Age Cage P Review By Owner Fl 1 861 F 2013 Cage Live Od Holding Review By Status vg 1 Review By Age Recently Added Animals 22 or Holdinc Selected Animals 0 26 amp Search Animals Haldine Measurements Lab Events Matings F neurogenin 1 777333 Black 6 ee 1 PTEN Holding E r EA A Ee ee neurogenin 2 77 7334 Holdinc Genes and Genotypes IACUC Protocols Vr e ED E ne Y SY ccs PUT Holdinc Billing Accounts
109. se Holding Type Setup Date 2013 09 26 Notes Cancel 58 Building Room Rack Row Column Locations BSRB Now a new cage has been created Animals To Be Moved 3 Swiss d Swiss 5 Swiss 6 Main Information The system will take the protocol information from the tar a A cage has been created Cage 777888 Click on Save and the animals will be moved to this new cage Note don t forget to click on Save or animals will not be moved to the new cage Tip When adding multiple animals and moving them to cages and you want to go back to the list of newly added animals you can click on Recently Added Animals 59 Cages Mo NENNEN List of Animals Displaying 1 22 of 22 records Page 1 of 1 Animals 9 Add New Animal 9 Add Multiple Animals vrAddto Selection New Update J Delete J Show Deleted Reco Review Animals P3 NO Animal Name Sex DOB Cage Project Background Review By Owner Fl 1 B61 F 2013 Cage Neurogenin k Review By Status 55d 1 DM Review By Age S Rar eee m 2 Be F 2013 Cage Neurogenin Recently Added Animals 23 ng PURI Selected Animals 0 20 tumar Search Animals Fl 3 Be63 F 2013 Cage Neurogenin EEEE E wi 1 ii bean 3 PERETE e eae 20 tumor Lab Events Soke RM E EE F 4 B64 z 3053 age NENGOEFUR See NR Dg 1 1 in brain Litters 26 tumor xU ene 5 865 F 2013 Cage Neurogenin
110. sed On Abbreviation Abbreviation Animal Tag Pups Animal Name Initial field will be disabled Animal Name Initial The animal name will be based on animal tag Enter the start number of animal Toe Clip tag you can leave this field blank and the system will create pups without names however you Mo of New Born 0 I Select if you want to create a litter record 161 will have to enter names before saving Pups Upon entering the number of newborns The system will create a table Mo of New Born Select Oif you want to create a litter record without pups You ci with the number of pups you selected No Animal Name Status Toe Clip Set th It will name the pups based on Actions Copy Auto Fill The re the start number of animal tag l ou chose y 1 Live 2 Tum 3 Live Jml 4 live 162 If you chose Based on Abbreviation for Animal Names Animal Name Initial field will be populated with the Abbreviation you chose in Project you can change the initial for this litter by entering a different initial You can enter the start number of animal tag optional Upon entering the number of newborns The system will create a table with the number of pups you selected It will name the pups based on the animal name initial It will also auto fill the animal tag numbers based on the start number you chose you can Main Information Reco
111. t the mating from Meurogenin 1 in brain tumor Review Matings List and click on Update or click on mating name and then click Edit W Search amp J Brow Setti Print 2 Barc wo rowse euiurnads rin Cages sa List of Matings Displaying 1 4 of 4 records Page 1 of 1 Animals Measurements P jer GSES dato cet TETTE FAdd to Selection New Gf Update J Delete Export Litter Lab Events Matings No Mating Parents Status Cat 9 Set Up Mating p i Test Mating 1 Male Female 1 Active 7000000 2 neurogenin 3 CDF 5 1 2 Active Weuragel Review Mating By Status m 3 neurogenin 2 CDF 9 CDF 10 male 7 Active cage a 4 E Molte t F neurogenin 1 neurogenin 8 neurogenin 10 CDF 7 CDF 8 Active Cage 2 c Search Matings zs Litters a Select an action Displaying 1 4 of 4 records Page 1 of 1 Projects 142 2 In Mating Information window you will see the existing dam s and sire Main Information Selected Cages Animals No Cages Animals Locations 7000000 W Maleg V Female 19 Building BSR Main Information Cage 7000000 Mating Details Mating Test Matine 1 Q Dam 4 A Female 1 Cj ire W Male Mating Date 2013 10 12 Plug Date 143 3 To add more dams click on the Dam magnifying glass icon Main Information Selected Cages Animals No Cages Animals Locations 7000000 V Maleg W Female 19 Building BSR Main Information Cag
112. the information and generate emails accordingly e Only one email will be sent on the same day for a given mating or litter i e no duplicate emails on the same day Example Delivery Date and Retire Date are set on the same day 201 Delivery Date 2013 10 20 Retire Date 2013 10 20 Eae Only one email will be sent on 10 20 e The Status of the Mating has to be Active m m e ae DITE Status Artin iil Notficatons Hates If you change the Mating Status to Completed or Canceled no emails will be generated e The Status of the Litter has to be Active Progress ls Active Yes gt Date of Birth 2013 10 13 Is Tagged No Tagging Date 2013 10 23 ls Genotyped Wo benotyping Date ls Weaned fo I Weaning Date 2013 11 03 202 If you change the Litter Status to Is Active No no emails will be generated e Ifa litter event has already been performed no email will be generated for that event For example if the answer to Is Tagged is set to Yes no Tagging reminder will be generated even if the Tagging Date is a future date Progress ls Active Yes Date of Birth 2013 10 13 Tagging Date 2013 10 23 ls Genotyped Wy Genotyping Date ls Weaned Wy Weaning Date 2013 11 03 Section 8 Lab Events Create New Event You can set up Events for yourself and also to share information with your lab members You can schedule the event date assign an event to s
113. tion New 2 Update Discard Cages J Delete E amp Holding 13 al Discarded ls 11 cage 23 Active 2013 10 male 8 r H Review By Owner 11 amp Breeding Q No Cage Name Type Status Setup Date MDA Cage 1 cage 33 Active 2013 10 CDF 6 Recently Added Cages 12 7 cage 8 Active 2013 10 CDF 10 Selected Cages 0 cage d Active 2013 10 CDF 7 C Search Cages 11 ll Cage Map F cage a Active 2013 10 male 7 114 3 In Discard Cage window Main Information enter Date Discarded Note by default if there are Cage 2 cage 23 cage 33 animals in the cage they will be automatically sacrificed If you do not want to sacrifice Main Information the animals uncheck the check Date Discarded 3973 10 14 box Sacrifice Animals in the Selected Cages 4 Click Save Date of Death 2013 10 11 Animals Sacrifice Animals In the Selected Cages Cause of Death A list of discarded cages will NONE display in Cages Review Create Cage Cages Discarded v Review Cages m Active 11 List of Cages Displaying 1 3 of 3 records Page 1 of 1 JF Add to Selection Chow f Update i Discard Cages a Delete Export E Breeding 0 No Cage Name Type Status Setup Date MDA Cages Anima You can make a discarded lal Holding 11 1 cage33 Discarded 2013 10 11 S Fi 2 cage23 Discarded 2013 10 11 and changing the Status to Active
114. ts Users Drop Down List Here you can set up drop down menu l Moace select a cateco ou like to edit Seton tor your lab Please select a category you like to edit Cage Building Reset Rack Room Type Animal Background Strain Cause of Death Coat Purpose Source Species Strain Mating Background Strain Category Source Strain 14 Drop Down List tab continued Please select a category you like to edit Background Strain For example select category Animal Background Strain Click on Add More Notice that if you delete a category the selected category will be disappear from your list only It will not affect your data You can define your category list here If you like to re order the values please select the box and drag it Background Strain Add More mixed Black 6 17 15 Drop Down List tab continued Please select a category you like to edit Background Strain A new field will appear Type in your new value and click Save You can define your category list here If you like to re order the values please select t Notice that if you delete a category the selected category will be disappear from your mixed Black 6 18 16 Drop Down List tab continued You can change the order of how values appear by dragging each field up and down You can delete a value by clicking on the x sign at the right 17 Drop Down List tab Cage Location It is important to set up all c
115. u 40 neu 41 NEU 42 neu 43 neu 34 CDF 8 Ma Iw male 107 neuro 1 neuro 3 Ma Iw neuro 7 neuro 8 neuro 9 Na Iw pup pup pup Na Iw Once you have selected the animals cages for the event 1 Enter a Title for the event 2 Enter Start Date and End Date for the event If this is a one time event select the same date for Start and End Date If this is an event that spans multiple days enter different days for Start and End Date 3 Enter Assigned To user You can assign this event to a Main Information Selected Cages Animals Ho Cages Ei Cage v 7000000 Event Details Project Animals 6 W neuro 7 IV neuro B IV neuro 9 Male IM Female 1 IM Female 2 W Female 3 Neurogenin 1 in brain tumor Title Start Date End Date Event Details Project Title Start Date End Date Assigned To Category Status Priarity 2013 10 13 2013 10 13 Neurogenin 1 in brain tumor BrdU injection 2013 10 30 2013 10 30 Naomi Iwai E Mew HM Normal 208 Locations Building BSRB Room SI Building BSRB Room SI lab member By default it is assigned to yourself 4 Enter Status Choose New or Proposed you can choose Proposed for an event that you want to assign to someone else 5 Set up Email Notifications mLIMS will send you email notifications forEvent Creation Event Updates and Event Reminders For more info
116. uired Optional Required Disabled This is the number on the Vet Med cage card Houston users Optional is recommended Smithville users Disabled is recommended Is Billing Account Required Yes No You will not see this question if you unchecked Access To Billing Account Management see Manage User Main Information Capes Cape Cards Animals Matings Litters Protocols Projects Users Drop Down List Time Zone GMT 06 00 Central Time US amp Canada Name Format First Name Last Name e 2 John Smith Currency Symbol Date Format Year 4 disits e g 2013 Day 2 digits with leading zeros e g 01 Date Example January 5 2013 2013 01 05 Main Information Main Information Cage Capacity ls MDA Cages Required Is Billing Account Required Cage Cards 5 Optional B No 5 11 BE dew Cdi rb es Bn aerae UR Animals Matings Litters Protocol The maximum number of animals per cage Permissions 4 Cages tab Cage Identifications Cage Identifications Leave this table as is don t enter default values as cage location can be set up in Project Encourage users to set up their own default cage location per project For more information see Section 4 Setting Up Your Projects Please define how you would like to record the locaton for eac Level Location Type Default Value Building Room Rack 5 Cages tab Cage Identifications e Efi at JH
117. user tries to assign an animal name that already exists in the lab Is Genotype Case Sensitive Please ignore as this is not relevant all genotypes are set up in Genes and Genotypes For more information see Set Up Genes and Genotypes 8 Animals tab Animal Animal Identifications Identifications Ma Animal Identificatiar Choose the primary method of animal dB MEER tagging in your lab If you don t see the right option in the drop down is Far Tag menu you can type in a new value Ear Punch Ear Tag Note the system currently does not E Micro Chip support the use of more than one i Toe Clip animal identification method per lab If more than one method is used in your lab please choose oneoption with a generic name e g ear toe tag 14 Default Value 9 Matings tab Allow Female Only Mating Yes No If you have matings for which you do not want to record the sire select Yes No of Dam per Mating No of Sire per Mating This depends on your lab practice e g if you use onlymatings that are one male one female you can set the number of dam per mating to 1 But if you use trio matings you can set it to 2 Date Calculations Set to Yes Delivery Days Enter a default number Note that delivery days can be set up per Project by each user which will override the number entered here in Settings Plug Days Enter 1 Main Information Cages Matings Allow Female Only Mating Ho af Dam per
118. vailable for all other records such as cages matings etc Note deleted records are removed from the active database Although you can recover them at any time your deleted items are never permanently deleted it is best practice to reserve the Delete function for erroneous records only When animals are dead use the Sacrifice function see Sacrifice Animals below or mark their status as Dead 1 In Review Animals List select List of Animals Displaying 1 10 of 10 records Page 1 of 1 the animals that you want to delete by checking the check e by checking the chec JF Add to Selection New i Update G ose Show Deleted Records Mating 2 sz OXes 2 Click on Delete NO Animal Name Status Sex Genes amp Genotypes DOB Coat neurogenin 1 Live FGFS Meurogenin 1 2013 10 het 09 neurogenin 2 Live FGFS Neurogenin 1 2013 10 null 0a neurogenin 3 Live FGFS Neurogenin 1 2013 10 null 0a neurogenin 4 Live FGFS Neurogenin 1 het 2013 10 09 neurogenin 5 Live F FGFS Meurogenin 1 2013 10 neurogenin 6 Live FGFS Neurogenin 1 2013 10 het na 71 3 You will see a message to confirm the Delete action 4 Click on Yes to proceed 5 Note that the deleted records are not visible anymore at be 4 tatic Sow Delete Selected Records Lanng amp fannivnac Are you sure you want to delete the selected records nat Far Punch f ane Deleted records will be hidden fr
119. ything with this animal such as mating updates etc as she does not have ownership of this animal and she does not have update permission to this record see below Alert sign this indicates that you have read only access to this record To allow Viji to use this animal and make updates Naomi can change ownership of this animal to Viji by simply updating the Owner field the ownership change will remain as a record in the Audit Trails tab under Animal Information 84 LOGOUT Admin My Calendar My Animals My Cages Environment Test HOME Hello Viji Chakravarthy Test Project Vij T J9 Search woul Browse rf Browse Deleted j Settings T Print Cages List of Animals Displaying 1 3 of 3 records Page 1 of 1 Animals 9 add New Animal l 9 Add Multiple Animals T Add to Selection Review Animals New Update Tj Delete J Show Deleted Records Exp No Alert Animal Name Sex DOB Owner By Owner 1 neurogenin9 M 2013 10 09 Naomi Iwai By Status M 2012 08 31 Viji Chakravarthy 2 F 2012 08 31 Vii Chakravarthy 2 By Age Review Animal Pedigree and Ancestry In Animal Information click on the Pedigree tab to review ancestry and pedigree information of the animal 85 Animal Edit V Create amp Search Summary Cage Mating Parents Litter Animals From Same Litter 1 Animals In Cage 20 Parents 2 Pedigree Parents Open All Close AL 4 g neu 111 M 2013 10

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