Home

perfusion systems temperature control amplifiers & stimulators data

1. NL 900D NeuroLog case and power supply 1 980 NL 905 Neurolog small case and power supply 1 251 n E E NLIOORK Neen E as Relay NLIOOAKS Headstage amp 1 928 NL101 NeuroLog Electrode Chamber specify pin and o d 71 NL102G NeuroLog DC Pre Amplifier with Pulse Buzz NL 412 3 005 NL104A NeuroLog AC Pre Amplifier 1 308 NL106 NeuroLog AC DC Pre Amplifier 1 308 NL120S NeuroLog Audio Amplifier 642 NL125 6 NeuroLog Filter Lo cut Hi cut 50 60Hz notch 1 163 NL136 4 channel Low Pass Filter 60Hz Notch 2 451 NL144 4 channel High Pass Filter 1 986 NL201 NeuroLog Spike Trigger 1 128 NL301 Pulse Generator replaces NL300 591 NL703 NeuroLog EMG Integrator 818 NL820A NeuroLog 4 Channel Isolation Amplifier 2 223 NL822 2 channel remote AC pre amplifier 1 946 NL822 P 2mm plugs with BLACK insulator for NL822 824 etc pack of 10 27 NL844 NeuroLog 4 channel AC Preamplifier 3 381 NL985S Loudspeaker for NL120S with safety plugs 192 AutoMate Scientific offers a complete line of Digitimer NeuroLog modules U S Canada prices shown International prices add 20 Email or visit web store for latest prices www autom8 com Eliminate 50 60 Hz Physical Standard steel instrument box with noise and harmonics anne e W 6 5 D 7 5 H 1 3 32 2 x 18 1 x 3 1 cm without filtering wantin Power e 115 120 VAC at 60 Hz e 230
2. Specifications Analog Inputs e Input channels 8 single ended e ADCs 8 e Sampling rates 1 Hz 500 kHz e Resolution 16 bit e Input range 10 to 10 V e Input resistance gt 1 MQ e Gain value 1 Maximum aggregate throughput rate is 500 kHz x 8 input channels 4 Megasamples sec Analog Outputs e Channels 8 e DACs 8 e Sampling rates 1 Hz 500 kHz e Resolution 16 bit e Output range 10 to 10 V e Output impedance lt 0 50 e Output short circuit to signal ground 25 mA Digital Inputs e Input type TTL compatible e Trigger Inputs e Input type TTL compatible e TAG rising edge sensitive e START rising edge sensitive Digital Outputs e Number of bits 8 of 16 supported in software e SCOPE dedicated trigger output e Output driver advanced CMOS AC compatible e Output current 4 mA source 32 mA sink Cable Type USB 2 0 braided Length 3 meters 90 AxoScope AxoScope software is turn key data acquisition and analysis software for Windows designed to replace oscilloscopes chart recorders and FM tape recorders AxoScope software provides up to sixteen channels of analog acquisition and four different acquisition modes Acquire data continuously in Gap Free mode with simultaneous display at up to the speed of the digitizer Set a trigger threshold for the Fixed Length Events Variable Length Events or High Speed Oscilloscope modes Tag and add comments to the data in
3. m E E E Ho 2 Besh e a a l j MED64 Mobius software Highly sophisticated user friendly software MED64 Mobius software is a data acquisition and analysis package for the MED64 system featuring a broad set of analysis functions available both online i e during acquisition and offline i e post acquisition It is designed to be easy for beginners and powerful enough for advanced users Mobius comes in various application specific packages including Evoked Potential Measurements Spike Sorter QT as well as combined packages for multi application users The Mobius user interface consists of various task specific control panels which can be quickly combined using a simple workflow editor to create custom experimental protocols and workflows However convenient pre defined workflow templates are available for users who want to quickly set up and run standard types of experiments 800 998 MATE Software Packages EP Evoked Potential measurement package Evoked local field potentials e g fEPSPs on all 64 channels can be recorded in response to customizable user defined stimulation parameters Parameters for acquisition and stimulation including stimulus current amplitude and wave forms are designed easily with a simple control panel Users can design and apply complex stimulation sequences independently for each stimulator Templates are included for delivery of repetitive e g theta
4. 800 998 MATE Q 2 lt L mD Major New Features Internal automatic mode switch Instantly switch recording modes by using the membrane potential as the trigger Pre set a voltage threshold in current clamp mode and the MultiClamp 7OOB amplifier will automatically switch to voltage clamp when the membrane potential reaches that voltage level A user specified delay will allow you to perform creative new experiments Oscillation suppression Sudden changes in membrane or pipette parameters may result in undesirable oscillations during whole cell recording The MultiClamp 7OOB amplifier will detect current or voltage oscillations and automatically disable or intelligently reduce compensation settings to protect your cell from damage Slow current injection Slight voltage drift often due to changing electrode properties may contaminate an otherwise decent current clamp recording In order to maintain the membrane potential at a consistent level the MultiClamp 7OOB amplifier will automatically inject compensatory current over a user defined time course 80 Axon CNS amplifiers for the quietest recordings MultiClamp 700B Amplifier Computer Controlled Dual Channel Resistive Feedback Patch Clamp and High Speed Current Clamp Amplifier The MultiClamp 7OOB amplifier is useful for a wide variety of intracellular or extracellular recording including e High speed Current
5. AUTOMATE SCIENTIFIC 13 21 53 ValveBank8 PTFE Inert perfusion system 3 995 Un regulated perfusion reservoir gas bubbler ooo fass oOo HEATED STAGE and PERFUSION CHAMBER PSs QuickStage Heated 12 mm diamond round coverslip QS H 12UP TT ZM chamber upright with ToolTray stage adapter for Zeiss Leica M and ThermoClamp temperature controller Build your rig on a solid foundation with TM 63 7590E CleanBench high performance lab air table 4 420 an industry standard TMC air table and T M 81 333 03 Type Il Faraday cage 1 610 Faraday cage for ideal vibration isolation TM 81 321 03 Full perimeter enclosure 300 U S Canada prices shown International prices add 15 20 Email or visit web store for latest prices 800 998 MATE 3 3 j E L L 4 E E i m ae a ir big m r E oe P Tai E i ii ie ao mi ii 8 4 T a r kla r i d r E a 4 dik i 1 a l Pir w z 4 ry a E E E po a k b oe re z seeibeer TEMPERATURE CONTROL SMARTSQUIRT y AN AutoMate Scientific PERFUSION SYSTEM PERFUSION CHAMBERS Table of Contents Perfusion Systems 6 ValveLink8 2 Controller 13 ValveBank Controller 15 Perfusion Accessories 17 EasyCode Software 20 Pressurized Superfusion Pressurized Superfusion amp Oxygen8 1 Perfusion Peniel Multi Barrel Manifold 23 BubbleStop Syringe Hea
6. EasyCode software to pre program Free Windows XP real time USB control and ValveBanks up to 16 ch networking software for up to 64 valves at once MANUAL CONTROL External keypad MANUAL FEATURES 1 on master channel timed open TTL outputs SOFTWARE Front panel buttons 1 on master channel SPEED 10 milliseconds 1 millisecond Up to 12 watts 1 amp per channel 24 watts VALVE POWER 4 watts per channel or 8 watts total 2 amps total PRICE Higher Lower ValveLink8 2 Controller Ordering Information 01 18 ValveLink 8 2 digital manual controller 995 01 26 ValveLink 16 2 digital manual controller 1 895 BNC cable ValveLink8 2 to pCLAMP Digidata et al oe 4 BNC plugs to DB 9 DBE BNC cable ValveLink16 2 to pCLAMP Digidata et al One 5 BNC plugs to DB 15 3o GE 01 29 USB cable USB A male USB B male 10 cable 9 01 30 USB hub 4 port unpowered 24 01 17 Rack mounting brackets ValveLink8 2 to standard 19 rack 20 01 25 Rack mounting brackets ValveLink16 to standard 19 rack 35 Cables for Heka InstruTECH and LabView call U S Canada prices shown International prices add 15 Email or visit web store for latest prices www autom8 com Behind every great We use the ValveBank in electrophysiology on a Xenopus oocyte recording rig We have D e r f U S O n syste m S g run a twelve channel manual perfusion delivery system fo
7. LLR AutoMate Scientific READY FOR RESEARCH m IJHUIIIS aeWo Ny 230105 CATALOG TEMPERATURE CONTROL DATA ACQUISITION MANIPULATORS amp PULLERS AUTO PATCH CLAMPING gt r lt e p r PERFUSION CHAMBERS gt VY l LL ox 2 BIOPHYSICS NEUROSCIENCE PHYSIOLOGY We build the tools so you can do the science You probably didn t go into biology to build your own electronics Why waste months working the bugs out of a home made perfusion system Spend your time learning how the brain works instead of soldering cables AutoMate Scientific has manufactured scientific instrumentation since 1992 We compete with larger companies by using our experience to build better products with more perfusion features you need Our engineers work constantly to create innovative new tools to extend your research while keeping costs down Each system is built for your specific application so you can start using it right out of the box We design quality into every product using first class components and suppliers to manufacture the best equipment possible Maximize value by ordering a complete system or save money buying just the pieces you need Our service doesn t stop until everything is working exactly how you want it to From anesthesia to Xenopus research scientists world wide place their trust in AutoMate Scientific s produc
8. O 1 8 i d hose barb 1 1 16 i d hose barb 2 Luer lock female with stopcocks 3 10 32 threaded nut amp ferrules for 1 16 o d tubing add 30 set of 4 p Pinch valves have no fittings www autom8 com Pom ane Our lab has been using Ten times faster ValveLink controllers for years with good reliable results New protection inputs The new ValveLink8 2 is even smaller and faster than its a nN d Ve nt m a r k eC r predecessor If also looks cool and the buttons feel nicer l Il b than the previous version IN sma er OX use if manually or programmed by outputs from my stimulator haven t observed any noise from the valve system on my electrophysiology rig Hillel Adesnik Ph D Department of Cellular and Molecular Pharmacology University of California San Francisco JMe Di Ere E Da 1 eae err P b FEF E i e N ValyveLink B 2 ay epee j e iat Adi bide oOo AuteMate Soentitic ValveLink8 2 Controller e ValveGuard technology detects bad valves Prevent damage to your ValveLink8 2 and easily observe problem valves e Run experiments automatically even unattended By running experiments automatically AutoMate Scientific systems will leave you free to accomplish other tasks saving you both time and money e Microprocessor based for accuracy and flexibility Our low cost ValveLink8 2 controller has powerful perfusion commands and capabilities not offered by com
9. The 8090c Platform The 8090c perfusion chamber platform is specifically designed with the electrophysiologist in mind With adequate mounting holes and a drainage trough around the perimeter for solution overflows The 8090c is ideal for fixed stage electrophysiology setups The drainage trough is designed to protect your expensive microscope optics by funneling the solution to the outer edge of the platform and down the drainage tube to a safe location The chamber receiver is located off of center and accepts our PC series perfusion chambers Clearance holes along the right and left hand edges allow mounting as a stand alone fixed stage or as the center chamber platform in a bridged platform setup The PC A chamber adapter allows the user to mount our PC V and PC H chambers into our 8090P platform Their 10 8 cm diameter is also compatible with Olympus microscope stages and allows 360 rotation of the chamber r Electrode Holders The new Stable tip electrode holders eliminate the final instability in the electrophysiology experimental setup We ve taken a thermally stable base material and coated it with aluminaoxide This coating has two benefits first it is non conductive so the holder does not act as an electrical antenna second it is very resistant to corrosion Under conditions of a high resistance electrode seal gigaseal the ST series electrode holder has a noise level of 0 79 0 80 pA RMS 5kHz f
10. The NeuroLog System provides AC or DC coupled amplification of biological signals from transducers single electrode or multi electrode configurations DC coupled amplifiers output absolute voltage levels and are most commonly employed for intracellular or transducer recording where baseline membrane potentials or slow voltage shifts are of interest 48 www autom8 com With AC coupled amplifiers the DC baseline is removed by high pass filtering Such amplifiers are used for extracellular recording of action potentials in neuronal preparations ECG EMG or EEG waveforms The variety of NeuroLog pre amplification and amplification modules means that users can develop systems specifically suited to their particular application The NeuroLog range also contains a number of filter and signal conditioning modules which can be used prior to final data acquisition Extracellular AC Recording The NLIOOAK head stage and NL104A AC PRE AMPLIFIER provide an excellent combination suitable for extracellular recordings from neuronal preparations with sharp electrodes They can be used in single sided or differential recording modes provide impedance matching for micro electrode recording and feature low noise amplification Continuously adjustable bandpass filtering from 0 1 Hz to gt 50 kHz is available through the NL125 6 FILTERS In addition the NL201 SPIKE TRIGGER can be used to convert spikes into uniform TTL pulses which can th
11. With its USB 2 0 interface you can easily connect the Digidata 1550A to a laptop computer without the need for a peripheral PC card Digidata 1550A Data Acquisition with HumSilencer Presenting the latest Digidata 1550A digitizer for low noise experiments This high resolution 16 bit data acquisition system is self contained and communicates with the host computer via a USB 2 0 interface which means extremely easy installation and setup Designed for ease of use and fast results the Digidata 1550A comes with versatile AxoScope for Windows software and is ready to take data immediately after installation Absolutely no programming is necessary The Digidata 1550A has a maximum sampling rate of 500 kHz per channel with an outstanding total data throughput rate of 4 megasamples per second Both the inherent digitizer noise and channel crosstalk noise are rated at less than 1 mV average p p at 10 kHz within a 10 V input range The front panel is well laid out with eight analog input channels and eight analog output channels eight general digital outputs one dedicated digital output to trigger devices such as oscilloscopes trigger inputs to start acquisition and to tag data The back panel has four additional analog instrument telegraph inputs as well as a DB 25 connector for the digital outputs 800 998 MATE 89 G D gt gt gt M 2 A S zZ zZ A D LJ lt L lt L lt L O
12. 1 7 mm second and medium 300 um second The third speed selector slow has a variable 330 potentiometer that enables settings from high speed 50 um second to low speed 2 um second With the speed selector set at the slowest settings consistent 0 2 um moves are easily made by the simple bump of an axis button All of the Siskiyou controllers on these two pages use encoder feedback from the motor to drive the device This encoder coupling enables the use of the DR1000 digital readout for repeated positioning requirements The MC1000e controllers also use a wall mounted power supply as their source for clean DC power All cables are shielded and a central ground lug is located on the junction box to ensure noise free operation during sensitive electrophysiology experiments osed Loop Dial Controller The MC1000e R T 4 axis dial controller acts as a remote micrometer control for 800 amp 7000 series actuators manipulators and stages It uses encoder feedback from our closed loop devices to create an electronic link between the controller dial and the device being driven This direct coupling to the encoder ensures smooth and coordinated motion between the controller and the drive A two position rocker switch is conveniently located on the top of the controller The rapid setting is set to maximize speed 1 7 mm second when the dial is turned at 18O RPM The slow setting is set to maximize resolution 0 2 um but sti
13. 88 The most widely used patch clamp data acquisition and analysis program CGM ee Ae Bj ujela j a Eee E p a eS winama Aee a eee A CA Saat a ee Sal al San San en i Eats pCLAMP 10 Software The pCLAMP 10 software suite fulfills many different experimental needs such as synchronized stimulation event detection and online analysis It is the most widely used data acquisition and analysis program for the control and recording of voltage clamp current clamp and patch clamp experiments Three separate programs are included Clampex 10 AxoScope 10 Clampfit 10 Clampex 10 expands the range and quality of your data acquisition experiments These improvements should prove useful for a wide variety of applications allowing more flexibility in your experimental protocols Requirements Minimum e Windows 2000 1 2 GHz CPU 512 MB RAM 500 MB hard disk 1024 x 768 display CD ROM drive 1 USB 1 1 port Digidata 1320 series digitizer 1 PCI slot full height Recommended e Windows 7 32 or 64 bit 2 GHz CPU 2 GB RAM 32 GB hard disk 1024 x 768 display CD ROM drive 3 USB 2 0 ports Digidata 1550A digitizer Network connection www autom8 com USB 20 interface soa ceca data acquisition within a faci means extremely easy a uals fully supported by our AxoScope installation and setup 10 for Windows and pCLAMP 10 for Windows electrophysiology software
14. For additional information and ordering please contact 800 998 MATE www autom8 com ay a This catalog printed on recycled paper with vegetable ink WG 2006 2015 AutoMate Scientific Inc ba
15. O LJ v a 2 O co e Prevents scratching the bottom of Slides e deal storage after oil immersion microscopy e Allows easy gas exchange through the bottom of u Slides e Stackable made of aluminum e Convenient parallel handling for up to eight Slides e Easy slide transportation from incubator to work bench e Preserves humidity temperature and gas concentration during incubation of cells inside the incubator e Can be combined with click racks shown e Inside dimensions fit multi well format 127 5 mm x 85 5 mm e Sterilization via alcohol water mixture Biomicroscopy Slides amp p Dishes Ordering Information www autom8 amp com e Very accurate volume delivery e No flow pulsation after initial start e Circulating flow available with push pull pump e Programmable flow rates and volumes available e Single and multiple syringe configurations e Accepts syringes from 10 ul to 140 ml 1g stages and incubation units e Universal heating system for all microscope platforms e Fits into a standard 96 well plate insert e Micro environment via heated plate and heated transparent lid e Heated transparent lid prevents condensation on u Slide lids e Inserts for u Slides regular slides or 35 mm dishes e Controller for two separate heating systems e Optional gas incubation unit e Analog heat controller available for stable microscope focus See large image on previous page
16. Stage adapter SEA QuickStage Heated 35mm WillCo Petri perfusion chamber QS H 12UP TT N Ba A O RUR QuickStage Heated 12mm diamond perfusion chamber perfusion chamber TT N 8 www autom8 com 4 Putting it all together Perfusion Packages To order a pressurized or temperature controlled perfusion system you should start with one of our unheated gravity systems and add the additional pieces you need Here are some examples Example Simple Systems Ordering Information 17 01 23 ValveLink8 PTFE inert Perfusion System 13 11 57 ValveBank8 Lee Valve 09 04 _ Perfusion PressureUpgrade Perfusion System 04 04 100 PerfusionPencilwith100umtip 01 05 __Low noise grounding 01 29 USB cable package 09 08 Perfusion Pressure Kit 13 11 57 ValveBank8 Lee Valve Perfusion System Brain Slice Chambers 03 18 360 ThermoClamp Inline Heater with 360um tip 10 8 60 G BubbleStop Syringe Heater Gravity VB 1600 Heka EPC 10 ValveBank Interface Cable Example Advanced Systems Ordering Information 13 01 53 ValveBank8 PTFE inert Perfusion System 01 03 2000 EasyCode ValveBank Programming Software S BSC1 Brain Slice Chamber 1 09 T18 Oxygen8 Bubbled Pressure Kit Top Half Interface and Submerged 10 8 60 G BubbleStop Syringe Heater Bottom 03 18 250 ThermoClamp Inline Heater with 250pm tip 01 15 ValveBank Parallel Port Cable to MetaMorph Accessories QS H 12IN Quic
17. e a j i TT ee J i r mmen eeg 1 S E r O e ery C i a a TT nt mes t Pressurized Superfusion For anyone who needs e Faster switching and steady flow e Fine microliter perfusion and microinjecting Easy to add to any new or existing gravity perfusion system from AutoMate Scientific any third party manufacturer or even homemade rigs Connect to house air or compressor 30 to 100 psi Does not introduce bubbles into solution helps overcome flow problems due to bubbles Available in four eight or sixteen channel configurations Syringe reservoirs may be placed in a water bath for temperature control Elegant design allows individual control of each air line 800 998 MATE Liquid Switching in 3 4 msec 2000 1800 8R 1600 1400 ji 1200 1000 800 600 400 200 ss eee sesssesgeege a a A a TIME 1 millisecond resolution Fast Flow Rhodamine B Fluorescence Switching 40 ms period using the AutoMate Scientific Pressurized Perfusion System at 2 psi Thilo Lacoste Materials Science Division Lawrence Berkeley National Laboratory Precision Components e 5 micron filter and gauge e Precision regulator delivers O 10 psi z juin ia r Ba bl 32 2 foe ee ie a Several sizes of plastic syringe reservoirs or 35 ml glass syringes and PTFE Inert tubing as our Volatiles Pressure Upgrade Rubber Stoppers Pressure Upgrade availa
18. rechargable Universal AC charger included Dimensions 190 x 210 x 40 mm Weight 510 g Power off recording mode USB computer interface Electrode holder and head stage adapters available 2 year warranty with free firmware updates Complete Sensapex product line available 115 2i lt L TE Y T IN TE o gt L lt L 2 TE PA Z lt Te u Specifications Major Components e Two micro manipulators each one includes a motorized linear stage and a manual XY stage One perfusion chamber and platform One PCI data acquisition board with BNC interface Mechanical error lt 0 05 mm Dimension 8 in x 12 in x 9 5 in Weight 10 Ib Line voltage 100 VAC to 120 VAC or 220 VAC to 240 VAC Motorized Stage e Travel 28 mm e Resolution 0 05 um e Maximum speed 4 mm s e Lowest speed 0 5 um s e Serial USB interface Manual XY Stage e Modular Dovetail Linear Stages e Set lock and forget less susceptible to shock and vibration e 0 5 in travel Data Acquisition Board e Sixteen analog Inputs 16 bit 250 kS s Input Impedance gt 10 GQ in parallel with 1OOpF e Two 16 bit analog outputs 740 kS s per channel Output impedance 0 2Q e 24 digital I O lines 32 bit counters digital triggering e Analog input range 10 V e Analog output range 10 V Perfusion Chamber e Accepts 1 16 tubing Computer Requirements e Windows XP with 2 0 GHz CPU e At least 1 USB port e 1 PC
19. stimuli Analysis of amplitude slope and area parameters can be performed automatically during or after acquisition and graphed independently for each channel An arbitrary number of measures can be enabled limited only by the processing power of your PC Spike Sorter Package Spikes are collected extracted and sorted on 64 channels on line and off line Spike time stamps waveforms of extracted spikes and spike frequency charts can be saved in ASCII text file csv format even WITHOUT saving raw data Raw data can be exported to binary or ASCII text file csv formats 111 gt U ae gt _ lt m CO On F N Q mT gt AJ m LL a lt L Li v f O LL 2 lt L zE A lt L Features e Ideal for recording and analysis of cardiomyocyte preparations including iPS ES cell derived cardiomyocytes primary myocyte cultures and acute heart tissue e Signals are recorded at any of 64 channels according to user defined acquisition parameters e Acquisition parameters and channels for analysis are selected easily in a simple control panel e Cardiomyocyte signals are automatically extracted according to user defined thresholds and analyzed for beat frequencies and inter spike intervals e Multiple types of slope amplitude time and area measurements can be performed automatically on extracted signals e Field potential duration FPD c
20. without penalty except for a 10 D m D z ay zZ oO ze gt zZ delivery Retain damaged containers for insurance claims Items received restocking fee for credit card should be inspected upon receipt and any shortages must be reported orae inet Davoaicaay Oren charged within 5 days of receipt Unopened and unused products can generally ME D EE be returned without cause within 30 days of receipt without penalty E S E except for a 10 restocking fee for credit card orders that have already been charged The customer is responsible for return shipping charges Catalog Product returns after 30 days without cause will be handled on a case by case basis at AutoMate Scientific s discretion A minimum 15 restocking fee will apply Invoiced shipping charges will not be refunded Special order items may not be returnable e Repairs Please contact AutoMate Scientific for any necessary repair work before returning equipment to obtain an RMA Return Authorization number Package any returned goods securely to avoid further damage Ship the package prepaid and insured with a written explanation of the problem to our address with the RMA number visible Out of warranty repairs will receive an initial estimate before work is begun e Catalog e Please visit our web store for international and updated pricing AutoMate Scientific has worked hard to provide the most complete and eee ener eee e Call or email us to
21. 39 ae Li co 2 lt LJ Zz Y D LL aa Li A LLJ gt LI Oocyte Chamber Diagram Microscope Adapter Suction Tubing Agar Bridge Oocyte Reservoir for Reference Electrode Integrated outflow tube and agar bridge e Chamber dimensions 45 x 30 x 10 mm e New slot for virtual ground electrode Oocyte Chamber Side View Insert micro manifold for very low dead space 40 Low working volume means fast exchange times Oocyte Perfusion Chamber e Small working volume lt 20 pl e Fast solution exchange Expression of recombinant receptors in oocytes has been a favorite choice for researchers to assess the pharmacology of signal transduction pathways Since the oocytes are 1 1 5 mm in diameter however fast solution exchange around the oocyte was difficult After several years of electrophysiological research we have designed a Xenopus oocyte perfusion chamber for use in automated and unattended experiments Combined with an automated perfusion system this chamber allows researchers to obtain dose response data quickly and easily Oocyte Perfusion Chamber Ordering Information Oocyte perfusion chamber specify 80 90 100 108 110 or 120 mm stage adapter Micro manifold also recommended OPC 1 185 U S Canada prices shown International prices add 15 Email or visit web store for latest prices www autom8 com Why transfer to coverslips sas 2 eae t
22. Controller Power Plug TTL Port Communicates with _ A Acquisition Host Automatically Analog Temperature Output V Temp 10 Changing Operating Modes from Dynamic to Imaging and Back a ie ne i Temp 10 During Imaging eated Lid Power Supply inged Perfusion Adapter Hinged Perfusion Adapter The Bioptechs Hinged Perfusion Adapter provides Delta T users with a convenient and inexpensive method of supporting perfusion needles in the culture dish The typical application is to maintain low volume perfusion over cells during long term experiments Perfusion adapters are sold in pairs One hinge and needle is a supply the other is a drain The balance between supply and drain can be maintained continuously with the use of the Micro Perfusion Pump Additional supports can be added to hold gas jets pH probes cooling apparatus or other items which do not require critical positioning Perfusable The Coverglass Lid is a cover for the Delta T Culture Dish to be used when imaging to create an optical surface onto the liquid in the dish This eliminates the optical effect of fluid motion at the air to liquid surface above the cells that causes the contrast of the image to change Therefore when acquiring a series of images in a transmitted light contrast enhancing mode of microscopy all images will have a uniform contrast Forming an optically flattened glass to media surface on the top of the cells eliminates this proble
23. FS DM6000 B DM5500 B DM6000 B DM5000 B DM4000 B E6OOFN BX5OWI BX51WI BX61WI Axioskop FS Axioskop 2FS Axio Examiner DMLFS Gibraltar BX50WI IX70 Axioskop FS Stage Type Mechanical Electronic Mechanical Microscope Translator Microscope Translator Detachable Single Plate or Non 3 Plate Non Detachable Non Detachable Non Detachable Narishige ITS N Narishige ITS O Narishige ITS O2 Narishige ITS O2 Narishige ITS Z Narishige ITS Z2 Narishige ITS Z3 Narishige ITS L Gibraltar BX Series IX70 FS Series AutoMate Stage Adapter UU Universal O Olympus Ring O Olympus Ring Axioskop 2 FS FS Series Leica DMLFS LFS Series SUTTER MT 150 Trapini Tower MT 1000 MT 78 MP 78 MT 78 N Nikon Ring ZK Zeiss K SISKIYOU N Nikon Ring Electronic Stages if the insert cannot be definitively identified the customer should test that the stage adapter does not interfere with stage components during motion Heated Environmental Lid for 35mm dishes Dual channel ThermoClamp 2 Temperature Controller Two independent channels for simultaneous heating of Environmental Lid or Perfusion Pencil inline heater and heated QuickStage Heated glass lid with perfusion and gas control attaches magnetically to the top of our 35mm dish holders Control the temperature of a large 30 mm coverslip lid to minimize condensation while maintaining gas and temperature control over your 35
24. PatchMAX 100A the ChannelMAX 100A patch clamp is noise free and low drift The computer controlled system eliminates human errors so the success rate of making gigaohm seals using the ChannelMAX 100A is over VO Run two independent patch clamp experiments It is very easy to change the configuration to run two independent experiments You can easily have two perfusion chambers on the same setup Automated two electrode voltage clamp For two electrode voltage clamp impalement is controlled by computer so no microscope is needed When the electrode enters the oocyte the membrane potential changes and the movement is stopped The user can define the membrane potential threshold and the delay to stop movement after the electrode enters the oocyte Save on equipment cost amp space This system is more cost effective than a traditional electrophysiology setup The equipment cost would be much higher to achieve the same functionality and productivity using a traditional setup Lower cost and better quality Save space As the system can be used for both two electrode voltage clamp and patch clamp less lab space is required You do not need two data acquisition systems All the data is on one computer so it is easy for you to compare Switching between patch clamp and two electrode voltage clamp is easy The system is designed so that it can switch from patch clamp to two electrode voltage clamp easily With all of the adjustable parts the
25. This eliminates the effect of series resistance in the bath grounding electrode and the bath solution It can also minimize the extent of DC voltage shifts resulting from changes in the bath solution or temperature Headstage pipette holders and replacement parts available for all Axon CNS Instruments headstages Please see our web page http www autom8 com amp_headstages html for complete parts list Headstages Model Cells HS non U type CV non U type HS amp CV U type 2 mm socket 1mm socket 1mm socket 3 eat P 2 mm socket 2 mm socket No adapter neede to 1mm pin to 1mm pin Clamp 1 MCB 1 MCO 1 E1 E2 MCW 1 APN 1 2200 063 APN 1 2200 063 Non U type recessed 1 mm socket 1 mm socket 1mm socket 1mm pins to 2 mm pin to 1 mm pin to 1 mm pin MCO 1 E3 Patch 1 APN 1 2200 083 APN 1 2200 062 APN 1 2200 062 xi Sia 1mm socket 1 mm socket to 2 mm pin to 1 mm pin No adapter needed Clamp 1U MCB 1U MCO 1U E1 E2 E3 MCW 1U Patch 1U APN 1 2200 083 APN 1 2200 062 E electrode input All ground connections on model cells and headstages have 2 mm sockets except the CV203BU headstage which has a1 mm ground socket 800 998 MATE Mechanical Mounting Options A 4 102 mm long removable insulated mounting rod is provided at no charge with most headstages Diameter is 5 16 7 9 mm unless 1 4 or 3 8 6 3 or 9 5 mm is specified by purchaser Additionally all headstages include an insulated mounti
26. amp com r Slice Keeper and Holder e Brain slice keeper for pre incubation of slices e Hooks into standard Buchner filter funnel beaker or optional Brain Slice Keeper Holder for bubbling carbogen into ACSF e Unique curved bottom design keeps bubbles away from slices while promoting fresh solution flow over slices e Needle valve for adjusting gas flow included i SYSEWVHI ANSSIL 8 3JHS NiIVdd O D O Ea Complete System S BSK S BSKH S BSK Combo Brain Slice Keeper Brain Slice Keeper Holder aD U1 am Ta a r ee ee em aD E W F rain Slice Keeper 4 aD NO e Holds four sets of brain slices aD on e Container with lid and integrated air stone for bubbling carbogen into ACSF Needle valve for adjusting gas flow included e Unique curved bottom design keeps bubbles away from slices while promoting fresh solution flow over slices Brain Slice amp Tissue Chamber Ordering Information S BSC1 Brain slice chamber 1 Interface and Submerged 2 364 a S BSC2 Brain slice chamber 2 Interface 1 650 ee S BSC3 6 Brain slice chamber 3 6 Six Channel Chamber 2 585 Ee S BSC3 4 Brain slice chamber 3 4 Four Channel Chamber 2 amp 3 ch avail 2 198 P S U net Slice Anchor or Harp 56 a S BSK1 Brain Slice Keeper 229 Ea S BSK2 Brain Slice Keeper 2 low 5ml volume 324 S BSKH Brain Slice Keeper Holder 129 S BSK Combo
27. and 50 C 10 8 60 G BubbleStop Syringe Heater Gravity When used with cold solutions i e stored in the refrigerator overnight inline heaters can create bubbles inside their tubing Many people mistakenly QuickStage Chamber believe these bubbles come from air leaking into the tubing but they actually N form because warm liquids cannot hold as much gas as cold solutions The solutions release gas when heated and cause bubbles which can interrupt your perfusion flow AutoMate Scientific has designed an economical syringe heater block called the BubbleStop which pre heats the liquids so they off gas before entering your tubing The AutoMate Scientific QuickStage is designed to fit directly into your microscope stage or platform to hold a perfusion chamber or 35mm Petri dish The heated version of the QuickStage uses AutoMate Scientific s popular ThermoClamp temperature controller to maintain your set temperature from ambient to 50 C QS H 35W TT UU QuickStage Heated 35mm WillCo Petri perfusion chamber Temperature Control Ordering Information Thumb nuts 03 14 xxx ThermoClamp Inline Heater gala plate 10 4 60 G BubbleStop Syringe Heater Gravity ool Tray 10 4 60 P BubbleStop Syringe Heater Pressure Cell chamber Coverslip 03 18 xxx ThermoClamp Inline Heater 10 8 60 G BubbleStop Syringe Heater Gravity 10 8 60 P BubbleStop Syringe Heater Pressure Heating insert
28. available 2 053 QS CS2440 Replacement 24x40 mm 1 coverslips box of approx 75 pcs 42 QS 35 N V Additional 35mm adapter rings 20 QS GSK xxx Replacement Gasket specify chamber pack of 5 48 Unheated QuickStage Unheated horizontal perfusion chamber and stage CITN adapter for Nikon microscope and Siskiyou platforms ee QuickStage Unheated 35mm Nunc holder Tool Tray and stage EO OSI NIRS adapter for Olympus et al microscope stages 9o a7 3 add l chambers many 35mm dishes amp stage adapters available 372 663 Heated Environmental Lid for 35mm dishes QS HLID with ThermoClamp 1 controller and bath sensor 1 855 with ThermoClamp 2 controller and bath sensor replacing the Cean ThermoClamp 1 included with any heated QuickStage chamber TE U S Canada prices shown International prices add 15 Email or visit web store for latest prices 800 998 MATE 33 My lab has been using the Cool Solutions device for over ten years and it has been a fantastic unit for characterizing the properties of thermosensitive TRP channels both native and cloned Its Peltier cooling and heating provide accurate and quiet thermal stimuli in our electrophysiology and imaging experiments with superior range speed and reliability Dr David Julius Department of Physiology University of California San Francisco The main controller is ready for 19 rack mounting size 2U 483mm w x 90mm h x 395mm deep 19 w x 3 5 ta
29. cm wide x same two thumb nuts on each side For heated cell chambers not 4 56 11 6 cm deep Rectangular stage adapters i e Zeiss and Universal unheated chambers or 35 mm dish holders Clamps also require include a rectangular Tool Tray 5 in 12 7 cm x 3 5 in 8 9 cm deep heating adapter QS U R TT O Zeiss K stage adapter showing QS U H C UU Round unheated chamber in rectangular Tool Tray with thumb Olympus round stage adapter and screws removed square Tool tray with StageHands 800 998 MATE holding an unheated horizontal chamber four small screws to hold the chamber gasket and coverslip to the Clamps left installed on a universal stage adapter right 29 i SYUSEWVHI NOISNd dd JYVISMIING Q7 14 7 19 FH f p NO OU ee On on aD am Zs NO WWJ vI N Li mm Microscope Configuration lt L E ZEISS Inverted Pa O N gt Li ad Li Q Li LO lt L N x a Upright 20 OF OLYMPUS Inverted Upright components during motion 30 Model Stage Type Detachable Single Plate AutoMate Stage or Non 3 Plate Adapter Axiovert 100 Mechanical Detachable Object Guide ZM Zeiss M Axiovert 135 Non Detachable ZK Zeiss K Axiovert 200 Mechanical Detachable Object Guide ZM Zeiss M LSM META Confocal Non Detachable ZK Zeiss K Electronic Non Detachable ZK Zeiss K Axio Observer Mechanical No
30. control essential to your experiments As the world leader in micropipette fabrication technology Sutter Instrument s advanced pullers are essential to biomedical research and emerging analytical techniques using sub micron probes and pipettes Their innovations in puller design include sophisticated velocity sensing circuitry to maximize reproducibility and the laser based P 2000 puller capable of pulling fused silica 78 www autom8 com ical Instruments Sutter Instrument has combined extensive years of motor control experience and in house precision CNC machined parts to create a line of robust and versatile optical products to serve the tremendous growth in photonics optical research and imaging techniques Their latest developments are the SmartShutter a full featured controllable shutter and the Lambda 10 3 controller capable of commanding up to three wheels and two shutters LNAWNYLSNI Y4dLLAS XenoWorks is a modular system designed to meet a wide variety of microinjection application needs Sutter Instrument s experience with precision motor control has culminated in this state of the art system including the XenoWorks Micromanipulator and Digital Microinjector for Zygote pronuclear DNA microinjection embryonic stem cell blastocyst transfer Intracytoplasmic Sperm Injection ICSI adherent cell microinjection and somatic cell nuclear transfer Sutter Instrument Ordering Information
31. current temperature for recording Different sizes of replaceable tips are interchangeable with our standard Perfusion Pencil The tips have microliter dead volume for rapid switching No messy water jacket is required but the separate high flow line is capable of heating a perfusion chamber with water jacket if desired Do you need to change your prep s temperature over the course of an experiment A programmable ramp and hold feature can automatically vary the setpoint over time The ThermoClamp system includes bath and Perfusion Pencil thermocouple sensors Automatic overtemp and thermocouple failure protection alerts you to any problems Incorporate temperature control into your perfusion rig with simple micromanipulator mounting Operation Connect multiple reagent tubes from any perfusion system to the heated Perfusion Pencil on a manipulator directed into your chamber If desired connect a separate buffer line to the high flow bath luer connection on the Pencil and the outflow to your chamber If your chamber includes a water jacket you can use the high flow line with a constant flow of water to heat the chamber Place the bath thermocouple sensor in the chamber Set your desired temperature on the controller and begin liquid flow The ThermoClamp monitors bath temperature and heats the liquids flowing through the Perfusion Pencil keeping the chamber at exactly the desired temperature Your perfusion system can quickly change
32. e Analog temperature output ThermoClamp 1 e Dimensions 11 5 x 2 x 7 e Weight 4 Ibs 1 8 kg Low noise designed for electrophysiology CE Zero Dead Volume Pencils All AutoMate Scientific Perfusion Pencils ThermoClamp SmartSquirt and regular Perfusion Pencils are now available in a Zero Dead Volume configuration with all internal tubes extended 2 cm out of the tip The ZDV Pencils do not use removable tips since all of the tubes go all the way to your prep This eliminates the possibility of backflow and decreases solution switching times down to nearly zero 29 Features e Easy to remove full syringes e Analog DC heating circuitry means low noise for electrophysiology e Completely shielded electronics plus external power supply e Spill proof design e Range ambient to 50 C liquid temperature e Mounts to standard 1 2 rod Available with gravity and pressurized syringes Off gassing with pressurized perfusion Note the BubbleStop is NOT sufficient to warm solutions for delivery into a perfusion chamber without an inline heater like our ThermoClamp The BubbleStop only helps stop bubbles 26 Stop bubbles by pre warming solutions lt e iy i a Finni CERERE ES n p E E n F i iiiaae T e Ei 7 i rth ins ee a hiili bjinipi sate BubbleStop Syringe Heater Bubbles in perfusion systems are caused by s
33. e Chemotaxis of slow migrating adherent cells e Migration assays with simultaneous cell tracking e 3 chambers on one slide for parallel assays e Linear gradients stable for over 48 hours e Made for high end video microscopy e New 3 D chemotaxis p Slide also available 120 www autom8 com Il 6 channel p Slide for flow assays with minimal volume e Flow assays with a minimum number of cells and or liquids mouse model e Parallel shear stress applications e Parallel screenings using multichannel pipettes e Smallest ibidi channel slide with highest shear stress e Easy connection to tubes and pumps via female Luer adapter Od O lt M oe Y oO lt l TA m Ta le y shaped for bifurcation studies and blood vessel simulation e Non uniform laminar shear stress e 30 and 45 angles to simulate the bifurcation of a vessel e Designed for arteriosclerosis research e Ideal for cell cell interaction studies and cell drug interaction screenings under flow conditions e Easy connection to existing tubes and pumps via female Luer adapters gt ide V Protein assay p Slide for parallel solid phase immunoassays e Extremely low sample volumes e Protein recognition and binding studies e Determination of binding constants based on fluorescence imaging e 10 ul channel volume saves reagents e Adapters for all different pipette types well for
34. e Removable net type insert e Sloped insert for small volume interface experiments e Low noise temperature control e Interface preparations e Templates for single or dual wells e Minimal fluid dead space e Stable recordings for many hours 800 998 MATE 57 BRAIN SLICE amp TISSUE CHAMBERS lice Chamber 3 e Four or six well chamber with independent lines e Interface or submerged preparations e Fluid level adjustable in each well e Removable nylon net and insert e High throughput pharmacological studies scope Stage Chamber 1 e 1 ml capacity flow through upright stage chamber e Unique profiled contour for rotary and swing type turrets for par focal viewing e Glass coverslip base e Easy removal for cleaning ope Stage Chamber 3 e Interface chamber for use on an upright microscope e Template for single or dual channel use e Glass coverslip base allows use of normal condenser optics e Upright or inverted microscope access to a well perfused perfectly flat and transparent in vitro slice preparation e Slice viability increased beyond 16 hours as demonstrated with LFP recordings e High mechanical stability high flow rate and Bernoulli effect to optimize nutrient access for slices nchor aka harp or U net e Stainless steel device with nylon mesh for holding slices down e Heavy gauge steel minimizes floating e Razor blade shown for scale www autom8
35. equipment accurately and easily by loosening two flathead screws www autom8 com A solid support solution stare e 2 5 to 12 0 inch vertical adjustability e Solid aluminum construction e Flexible mounting design e Vacuum compatible versions available upon request PRS Series AS Series Platform Support Rods amp Spacers Platform Support Rods The PSR series platform support rods are a solid solution for supporting platforms or other mechanical hardware Our PSR platform support rods are specifically designed to be used with our 1200 and 12000 series platforms PSR platform support rods are cross drilled to accept a 3 16 hex wrench This cross hole enables secure tightening to AS spacers isolation table tops or other mechanical devices When used in conjunction with AS aluminum spacers accurate placement of our platforms manipulators or other devices is easily achieved Aluminum Spacers Use AS aluminum spacers for spacing of any device that needs to be adjusted in either the vertical or horizontal plane AS spacers can be used by themselves or with ABP series base plates They are also useful for added height adjustment on top of our PSR platform support rods For horizontal applications use our BB 1 0 building block cube attached to the top of an AS spacer then bolt another AS spacer to one or more of the four sides Individual size spacers are available in packages of 4 The assortment kit AS 1A includes one
36. ideal for axial cell probing To make probe replacement efficient the SD MX110 can be rotated away from the microscope and returned back to the original position using an adjustable stop Color coded knob caps are used to identify axis location in low light conditions Siskiyou Manual Manipulator Ordering Information SD 10 A axis mini micromanipulator specify right or left hand 555 SD MX110 Manual post mount 4 axis micromanipulator 1 460 SD 130 4 axis compact micromanipulator 750 SD 160 4 axis full micromanipulator 1 260 U S Canada prices shown International prices add 20 Email or visit web store for latest prices www autom8 com Exceptionally smooth linear travel and drift free operation ah Siskiyou Motorized amp Hydraulic Manipulators Siskiyou manipulators are well known for their reliable performance in electrophysiology recording and stimulation Motorized manipulators are available with push button joystick dial and computer controllers for single or multiple manipulators Headstage and electrode mounts are available for major amplifier brands Manipulators Four axis Base Mount The MX7600 motorized crossed roller bearing micromanipulator is ideal for patch recording experiments Offering exceptionally smooth linear travel and a precision preloaded lead screw to ensure drift free operation The motorized 4 axis micromanipulator incorporates our MXC 45 pipette holde
37. immunofluorescence and high end microscopy e Cultivation and microscopy of cell cultures e Fluorescence microscopy of living and fixed cells e Low evaporation long term assays e ibilreat surface for optimal cell adhesion e One piece system no peel off from the bottom e Excellent optical quality e No glue no leakage no cell harm 800 998 MATE 121 BIOMICROSCOPY p SLIDES Cell microscopy on or in 3D gel matrices Creates a flat gel surface with a minimum use of gel Tube formation assays Immunofluorescence staining 3D cultures Flat gel surface all cells in focus Homogeneous 0 8 mm thick gel layer Use only 10 ul of gel per well le 2 x 9 well for co cultivation assays Co culture experiments with different cell lines or primary cells Scratch assays with co cultures Cultivation and microscopy of cell cultures In situ cell experiments without cell transfer Two major wells each with 9 minor wells Cells share soluble factors but grow separated Low evaporation for long term assays Excellent optical quality ide 18 well flat for matrix tests or spotting samples such as RNA assays Quick immunofluorescence stainings of adherent cells Optimization of surface functionalizations Fast toxicological screening of small microscopy samples 18 fold sample preparation Compatible with multichannel pipettes well Plates for high throughput applications Fluorescence bas
38. input signal Indicator TOO FAST Red LED flashes if a Trigger is received while the unit is busy e Duration Total range 10 ms 12 seconds in three overlapping ranges Control Single turn control marked 1 12 sec with intermediate integer panel marks Accuracy 1 at 1 and 12 marks 5 at intermediate marks Multiplier xO 01 xO 1 x1 Internal Jumper Enable x10 giving a 100 ms 120 sec total range The front panel provides a check box to mark with a waterproof pen when enabled e Repetition Frequency Total range 0 01 1200 Hz in five overlapping ranges Control Single turn control marked 1 12 Hz with intermediate integer panel marks Accuracy 1 at 1 and 12 marks 5 at intermediate marks Multiplier xO 01 xO 1 x1 x10 x100 Isolated Stimulators Ordering Information DG2A Train Delay generator 914 U S Canada prices shown International prices add 20 Email or visit web store for latest prices www autom8 com Versatile precision engineered tools for long service Brain Slice Chamber Diagram Key Diagram below 1 Bubble trap 2 Heat exchanger for perfusion fluid submerged interface mode 3 Heat exchanger for perfusion fluid to sloped insert 4 Control temperature sensor 5 Heating element 6 Oxygen carbon dioxide gas bubbler 7 Exit for perfusion fluid via suction line Brain Slice amp Tissue Chambers e Interface and submerged preparations
39. ium imaging perfusion system e Deliver three solutions into six independent u Slide VI channels e Programmable switching and waste collection e Complete system with stand valves controller and tubing e Ideal for automated Calcium imaging studies r S i lide perfusion system e Deliver three different solutions into three independent u Slide III 3 into 1 channels e Programmable switching and waste collection e Complete system with stand valves controller and tubing e Control or reverse a chemical gradient across the u Slide e Add an optional wash or spacer solution between the gradients 800 998 MATE 125 Od O 2g O Y S W lt gt mI U Y O V mI Ta Customer Service Contact Information US Phone 800 998 MATE or 800 998 6283 Telephone 510 845 6283 Fax 510 280 3795 Email into autom8 com a Web Site www autom8 com PDF Brochures www autom8 com pdf_brochures html aa Web Store www autom8 com shop A International Distributors www autom8 com contact_international html eg Customer Service www autom8 com contact html E E Terms and Conditions Our knowledgeable and friendly Customer Service Representatives A summary of AutoMate Scientific s Terms and Warranty appear below are ready to assist you Monday ert ae E Our complete terms can be found at http www autom8 com terms html e Prices amp Payment Credit Ca
40. of the QuickStage uses AutoMate Scientific s popular ThermoClamp temperature controller to maintain your set temperature from ambient to 50 C Disassembly and cleaning of the heated chamber is just as simple as the unheated version The ferro stainless Tool Tray can be removed with all of your tubes and electrodes intact A silicone gasket ensures leak free performance without messy grease Stage heating is maintained while coverslips are replaced so you can begin recording again sooner 800 998 MATE 27 SS fo eee rE ae 5 12 mm chamber All of these chambers take a 24x40 mm coverslip on the bottom One included Adapters for Nunc WillCo Falcon In Vitro D35 10 1 N Corning Mattek and In Vitro D29 10 1 N 35mm dishes available 28 QuickStage Perfusion Chambers Standard laminar flow chamber Standard laminar flow chamber for inverted microscopes QS H 12IN for upright microscopes QS H 12UP also accepts 12 mm round coverslips also accepts 12 mm round coverslips shown with separate inflow manifold 2 in 5 cm approx 124ul approx 1037ul Small laminar flow chamber Large bath or slice chamber for inverted microscopes QS H 5IN for upright microscopes QS H LS also accepts 5 mm round coverslips shown with separate inflow manifold Unheated Perfusion Chambers te VQ j A ff T i lei for 22mm gt L Recessed for 22mm places square cover sli Recessed squ
41. patch clamp recording The CV 203BU headstage includes two feedback resistors to provide a wide range of current passing capacity in the whole cell mode The 500 megaohm feedback resistor B 1 provides both low noise and a large current passing ability 20 nA For larger currents one can switch to the 50 megaohm feedback resistor B 0 1 to pass up to 200 nA G5 gt gt lt O a gt 2 SR ale Genuine Axon CNS components for every experiment N Z LL Z Q Q 2 Q LJ N Z LJ 2 Q gt lt lt L Optional CV 5 Series Headstages for the GeneClamp 500B amplifier e CV 5 100GU patch clamp headstage 100 mV pA e CV 5B 100GU bilayer patch clamp headstage 100 mV pA e CV 5 1GU macro patch headstage 1 mV pA e CV 5 100MU voltammetry headstage 100 mV nA CV 5 headstages operate with the GeneClamp 500 amplifier Each headstage has one feedback resistor for current to voltage conversion The transfer resistance of the 100G 1G and 100M versions are 100 GO 1 GQ and 100 MQ respectively The B version has an extended capacitance compensation range suitable for bilayers The 100G version is ideal for single channel recording and vesicular release amperometry The 1G version is suitable for macropatch applications The 100M version is used for fast cyclic voltammetry using carbon fiber microelectrodes 86 Headstages holders model cells cables odd bits HS 2 an
42. powder paint 800 998 MATE Panel Rail Mounted Heavy Duty Shelf e Allows easy rack mounting of narrow equipment or items that do not have rack mounting capabilities e Shelves feature formed 14 gauge steel construction e 15 Deep x 3 5 2U high 100 pound weight load rating e Rear lip stiffener folded up for extra strength amp acts as a rear equipment retainer e Mounts to rack rails either as an internal shelf or external shelf work area e Rugged textured powder paint finish e Requires mounting hardware Flat Pack Shipping Shipped in a flat pack container for handling ease amp protection Perfect for tight access areas or 1 person installations Rack is knocked down into 5 easy to handle parts ie two sides top bottom and a pack of 4 panel rails Panel Rails 91 m 2 U Ea m Z ze gt am A X u lt L oz Q C T TE Z co lt L Light Duty Shelf e Allows easy rack mounting of narrow equipment or items that do not have rack mounting capabilities e Mounts to rack rails either as an internal shelf or external shelf work area e g for a keyboard e Shelves feature formed 16 gauge steel construction e 7 Deep x 1 75 1U high 40 pound weight load rating e Front amp back edge folded acting as both stiffeners and equipment retainers e Shelves include tie down slots on each side amp four tie down holes e Rugged textured black pow
43. real time Set analog output holding values Open Axon format ABF data files and quickly analyze sections of interest with an array of browsing and basic analysis tools Preview data and page layout before printing Additional features include voice tags which allow tagging of data with spoken comments requires a microphone and sound card low pass and high pass digital filtering of incoming data and Store Trace which freezes a snapshot of a waveform on the screen for comparison with subsequent input The Digidata 1550A rack mountable main unit comes standard with e USB 2 0 cable e External auto switching power supply e Power cable e AxoScope 10 software CD e Printed manual MDS Analytical Technologies Axon CNS Ordering Information MultiClamp MultiClamp 700B computer controlled current amp patch clamp amp 17 629 Axoclamp Axoclamp 900A computer controlled current amp voltage clamp 11 779 Axopatch Axopatch 200B 2 capacitor feedback patch clamp amp 15 099 SoftPanel SoftPanel USB optional control panel 2 119 Digidata Digidata 1550 data acquisition system 6 799 Digidata 1550A Digidata 1550A data acquisition system with HumSilencer 7 539 pCLAMP pCLAMP 10 Standard electrophysiology software Windows 6 029 pCLAMP Upgd pCLAMP 10 Upgrade available for previous versions of pCLAMP 539 Axoporator Axoporator 800A single cell electroporator 10 119 Mo 1 CV 7B Headstage CV 7B patch cl
44. specifically designed for live cell microscopy Now you can have accurate temperature control and high numeric aperture compatibility in a convenient disposable culture dish system that even works in confocal applications e Easily adapted to a variety of specimen types from monolayered adherent cells to brain slice and tissue preps e Low mass to thermo regulate as opposed to conventional stage heaters e Plate incubate and observe without the need to transfer your cells e Fast thermal recovery after perfusion within seconds e Compatible with inverted and upright microscope stands e Coverglass bottom for optimum optical compatibility e No need for warm air blowers or stage heaters e Direct first surface heating to your cells e Can also be cooled below ambient e Perfusion available e No pre heating Bioptechs is now introducing the next generation Open Culture Dish Micro Environmental Control System the Delta T4 In addition to the improvements to the popular Delta TC3 control algorithm Bioptechs has incorporated years of customer requests into the Delta T4 800 998 MATE 101 Limitations of Traditional Technique e Stage heaters are inefficient slow and inaccurate Plastic dishes are poor conductors of heat Temperature does not recover quickly during or after perfusion Plastic dishes are not suitable for high resolution or polarization microscopy Nonuniform temperature distribution Unnecessary
45. system can easily be adapted to different glass electrode lengths and sizes and different amplifiers Use with traditional two electrode voltage clamp experiments It is possible to use the ChannelMAX 100A Mini with a traditional two electrode voltage clamp to run two two electrode voltage clamp experiments simultaneously High efficiency less labor intensive As the system is automated it is easy to run experiments in parallel to increase efficiency Patch clamp and two electrode voltage clamp should not be labor intensive Let the equipment do the hard work for you 800 998 MATE 117 M a5 gt A m z gt gt L QO m py GC Y m gt 2 lt L TE Y T IN TE o gt L lt L 2 TE PA Z lt e u Specifications Major Components Four micro manipulators each one includes a motorized linear stage and a manual XY stage Two perfusion chamber and platform One PCI data acquisition board with BNC interface Mechanical error lt 0 05 mm Dimension 12 in x 12 in x 9 5 in Weight 15 Ib Line voltage 100 VAC to 120 VAC or 220 VAC to 240 VAC Motorized Stage e Travel 28 mm e Resolution 0 05 um e Maximum speed 4 mm s e Lowest speed 0 5 um s e Serial USB interface Manual XY Stage e Modular Dovetail Linear Stages e Set lock and forget less susceptible to shock and vibration e 0 5 in travel Data Acquisition Board e Sixteen analog Inputs 16 bit 250 kS s
46. tubes are all plastic to protect your cells See the ThermoClamp page Incubator Culture cells on MED64 probes in our incubator With our modification you can even record from MED64 probes while inside the incubator Alpha MED is the only commercially available MEA system suitable for recording while incubating 113 gt a gt z lt m CO On F m AJ a C Y C Z Integrated Digitizer e 40 kHz sampling rate 16 bit A D 18 bit internal resolution Stimulus voltage ranges 250 mV and 2000 mV Zap voltage range of 1000 mV Feedback Gain Settings 10 MQ 100 MQ 1 GQ 3 3 GQ 10 GQ Low RMS Noise DC to 3kHz e 0 3pA 10GQ 1 0pA 1GQ 7pA 100M2 Compensations e Up to 4 Cap Compensations Cfast x 1 Cslow x 3 e 0 100 pF per compensation e Series Resistance Compensation Offset Compensation 250 mV Optional Active Leak Compensation Current Clamp e 2 nA range with 1 25 pA res e 20 nA range with 12 5 pA res e 200 nA range with 125 pA res i 1o0pA L a 10ms_ Model Cell The patented Spread Frequency Compensation can automatically compensate any arbitrary capacitance profile in approximately 3 seconds 114 The smallest patch clamp system available USB powered Miniature Patch Clamp Amplifier and Digitizer Full featured 1 channel amplifier Pico is a feature rich low noise patch clamp amplifier with an integrated digitizer and head
47. with heated continuous perfusion AutoMate Scientific offers a wide array of perfusion options and accessories for the Aloha MED64 system The Perfusion Cap shown above is ideal for both gas and solution delivery and removal For applications requiring an open top such as imaging or conventional microelectrode electrophysiology the Perfusion Pipes shown in the side column make perfusion inflow and outflow easy Both of these options can be used with AutoMate Scientific s widely used perfusion systems and ThermoClamp inline heater Choose a complete multi channel drug delivery system with rapid valve switching under computer control or just a single heated solution line into your probe We can configure gravity air pressure or peristaltic pump driven liquid delivery and removal Take a look at the Perfusion System chapter of our catalog or visit http www autom8 com build_your_own html Take the guesswork out of your multielectrode array system with AutoMate Scientific s perfusion experience 800 998 MATE Perfusion Pipes The Perfusion Pipes are ideal for liquid delivery and extraction when you need the top of the MED64 probe open for imaging or microelectrode electrophysiology Peristaltic Pump Dual channel peristaltic pump for solution delivery and removal ThermoClamp Inline Heater Inline solution heater pre heats liquids as they enter the probe Available with multiple tubes for rapid switching Internal
48. x 4 0 inch tall 50 SD PSR 6 0 1 5 diameter platform support rod 6 0 inch 55 SD PSR 10 0 1 5 diameter platform support rod 10 0 inch 70 SD AS 1A Aluminum spacer kit assortment 25 SD AS 125 Aluminum spacers 125 pkg of 4 17 SD AS 250 Aluminum spacers 250 pkg of 4 18 SD AS 500 Aluminum spacers 500 pkg of 4 20 SD AS 1 00 Aluminum spacers 1 00 pkg of 4 23 SD AS 1 50 Aluminum spacers 1 50 pkg of 4 25 SD AS 2 00 Aluminum spacers 2 00 pkg of 4 28 SD AS 4 00 Aluminum spacers 4 00 pkg of 1 30 SD AS 6 00 Aluminum spacers 6 00 pkg of 1 35 SD PSR 100 1 5 diameter platform support rod 100 mm tall 50 SD PSR 150 1 5 diameter platform support rod 150 mm 55 SD PSR 250 1 5 diameter platform support rod 250 mm 70 SD AS 25A M Aluminum spacer kit assortment metric 25 SD AS 3 Aluminum spacers 3 mm pkg of 4 17 SD AS 7 Aluminum spacers 7 mm pkg of 4 18 SD AS 015 Aluminum spacers 15 mm pkg of 4 20 SD AS 25 4 Aluminum spacers 25 mm pkg of 4 23 SD AS 40 Aluminum spacers 40 mm pkg of 4 25 SD AS 50 Aluminum spacers 50 mm pkg of 4 28 SD AS 100 Aluminum spacers 100 mm pkg of 1 30 SD AS 150 Aluminum spacers 150 mm pkg of 1 35 U S Canada prices shown International prices add 20 Email or visit web store for latest prices 64 www autom8 com Move the microscope not the experiment Manual amp Motorized Microscope Translator MXMS 1
49. you apply pulses to break into the cell The ez gSEAL control software helps you measure the pressure you use for making seals and breaking in In addition many types of cells and tissues such as HEK293 cells brain slice and oocytes have been tested and seal parameters are available for them New users can usually use these parameters to make seals and break on the first attempt When used with the ezPATCH 100A manipulator all you need to do is to aim the patch pipette at the cell you want to patch After you click a button it will touch the cell and make seal or even go whole cell automatically ChannelMAX 100A amp ez gSEAL Ordering Information Ne ChannelMAX 100A Twin ChannelMAX 100A Twin 33 400 Ne ChannelMAX 100A Mini ChannelMAX 100A Mini 18 200 Ne ez gSEAL ez 3SEAL Pressure Controller 5 100 Complete NeoBiosystems product line available U S Canada prices shown International prices add 10 Email or visit web store for latest prices 800 998 MATE Specifications Pressure range 250 mmHg to 250 mmHg Pressure resolution 1 5 mmHg Minimal pulse duration 7 ms Minimal pulse interval 7 ms Computer interface USB Line voltage 110 to 240 VAC Dimensions 17 x14 x3 5 rack mount or desktop Weight 10 Ibs Computer Requirements e 32 bit Windows 2000 XP Vista or Win 7 with 2 0 GHz CPU e 1 USB port Pressure control for stretch channel study The ez gSEAL pressure c
50. 0 TPI 5 um e 80 TPI submicron e 100 TPI submicron Motorized Backlash lt 5 um Point to point accuracy 2 um Economy Translator e MXMS 100 series translators have a full 25 mm of XY translation and use ball transfer pads in the back two bearing locations instead of full crossed rollers Corre Fart VEM Har ee HENT Hiari be 65 66 Stability and full features at a great price Manual Base Mount Micromanipulators e Four axis control with soft touch knobs on all models shown e Five or ten micron accuracy e Combine with a Perfusion Pencil for focal drug application The AutoMate Scientific Perfusion Pencil makes a perfect companion to these four axis micromanipulators Mount the Perfusion Pencil for micron accurate drug delivery and rapid rotational change out The small SD 10 manipulator offers 5 um resolution Its compact size makes it ideal for stimulator applications The SD 130 manipulator is designed to maximize available space Its narrow profile allows several manipulators to be placed in one quadrant With a long 22 mm 0 86 inches of travel on its probe axis and X axis the SD 160 is ideal for axial cell probing It includes an adapter mounted to the axis with a rotational stop for convenient electrode pipette replacement www autom8 com D 130 Narrow Space saving Manipulator The SD 130 Manipulator is designed to maximize available space The narrow profile design allows multiple manip
51. 00cr series microscope translators are designed to be stable XY translators for fixed stages experiments MXMS 100Ocr series translators have a full 25 mm of XY translation and have full crossed roller bearing stages in all three locations MXMS crossed roller translators are available in motorized and manual versions Motorized versions can be controlled with any e series and MC2000 controllers Controllers drive the stage through a closed loop interface between the controller and the motor encoder The closed loop connection ensures 0 2 um and 0 1 um resolution respectively The encoder coupling also enables the use of the DR1000 digital readout for repeated or relative positioning requirements All cables are shielded to ensure noise free operation during sensitive electrophysiology experiments Manual versions can be fitted with either 20 40 80 or 100 TPI adjustment screws with resolution from 20 um down to 5 um respectively MXMS 100cr series stages can be sized to fit most microscopes Please include the manufacturer name and model of your microscope when ordering A clamp kit appropriate for your microscope will be included 800 998 MATE Features e 20 40 80 or 100 pitch drive screws e Compatible with fixed stage microscopes e Full crossed roller stage support Performance Specifications e Maximum load 200 lbs centered e Travel axis 1 0 inch 25 mm e Minimum controllable motion e Manual 20 TPI 10 um e 4
52. 1 each of AS 0 125 AS 0 25 AS 0 50 AS 1 0 AS 1 50 amp AS 2 0 spacers size plus two 2 1 4 20 x 5 8 stainless steel set screws for attaching the AS 1 50 to the AS 2 00 800 998 MATE 63 Siskiyou Fixed Platform Ordering Information LY a PartNo Product Description Price SD BB 5 0 Building block 5 0 inch 17 SD RTC 0 5 Rotation translation clamp 0 5 inch 22 lt L SD RTC A3 RTC adapter 1 4 20 or M6 clearance pkg of 3 16 aE SD PH 1 Platform hardware kit assortment 20 SD 8090P Platform 8 0 x 9 0 10 8cm aperture 290 SD 1280P Platform 12 0 x 8 0 beveled 280 Z SD BB 1 Building block 1 0 inch v Siskiyou Manual amp Motorized Platform Ordering Information SD MXZPi 2 Motorized platform with one 200Ocri motorized crossed roller XY 9 400 stage and one floater 2OOcr crossed roller XY stage SD MXZP Manual platform with one 10Ocr XY stage with either 20 40 80 7 200 or 100 TPI adjustment screws and three floater 10Ocr crossed roller XY stages Siskiyou Tower Ordering Information SD MX312 P Coarse vertical adjustment platform 3 0 x3 0 315 SD MX312P T MX312P top plate 85 SD MX312P B MX312P base plate 125 Siskiyou Spacer Ordering Information SD PSR 4 0 1 5 diameter Platform Support Rods
53. 1 9 2 0 2 2 2 4 2 6 4 8 5 0 5 5 6 0 6 5 9 5 and multiples of 10 thereof Gain Range 0 1 to 10 000 Steps 0 1 0 15 0 2 0 25 0 3 0 4 0 5 0 6 0 7 0 8 0 9 then as above for gains 1 0 to 10 000 Gain Accuracy lt 2 error Lowpass Filter Range 20 Hz to 50 kHz 8 pole plus 80 kHz 2 pole and wideband Steps as above for frequencies 20 Hz to 15 kHz then 18 20 22 25 33 40 50 80 all kHz and wideband Characteristic 8 pole Bessel 8 pole Butterworth may be substituted on any or all channels contact AutoMate Scientific Wideband Frequency Response 500 kHz min all gains 46 High performance four channel amplifier and signal conditioner in one quiet package a a e D H Brownlee Precision Model 440 Amplifier The Brownlee Precision Model 440 incorporates four channels of sophisticated amplifier and filter circuitry in a friendly easy to use instrument Each channel consists of a high gain low noise amplifier an 8 pole Bessel lowpass filter a highpass filter a line notch filter and output offset controls A control knob sets the amplifier parameters and the values are displayed on a bright LED alphanumeric readout e Key Features Clear front panel controls for all settings Gains from 0 1 to 10 000 in fine calibrated steps Single ended or differential inputs 8 pole Bessel lowpass filter with range 20 Hz to 50 kHz H
54. 240 VAC at 50 60 Hz Input Voltages Input protection 50 volts peak to peak Maximum input signal recognized by the adaptor 5 volts peak to peak Maximum noise amplitude for complete cancellation 1 volt peak to peak Frequency Response Input to output DC to greater than 500 kHz Hz and harmonics cancellation Hum Bug Noise Eliminator 50 60 Hz to 4 kHz e Eliminates electrical interference Simple 50 60 Hz sine waves Mixtures of 50 60 Hz harmonics Noise spikes from dimmers Complex noise from fluorescent lamps e No waveform distortion No frequency loss or DC voltage shift No signal attenuation or phase error The Hum Bug is a powerful device for canceling electrical interference in real time avoiding all of the traditional problems associated with notch filters The Hum Bug constructs a replica of noise present on the input signal and continuously subtracts this replica from the signal as it passes through the instrument It performs this function in the presence of biological activity even when noise characteristics evolve over time 800 998 MATE 51 Controls Bypass halts noise cancellation by routing input directly to output Hold suspends adaptation to evolving noise characteristics Clear clears the noise replica Display LED indicates changing noise levels e Green decreasing amplitude of the noise replica e Red increasing amplitude of the noise replica a2 The Hum Bug is not a filter It
55. Brain Slice Keeper and Holder 358 S BSK4 Brain Slice Keeper Holder Four Holders in one container 663 S MSC1 Microscope stage chamber 1 Submerged for upright microscopes 509 S MSC3 Microscope stage chamber 3 Interface 1 257 S MSC4 Microscope stage chamber 4 Submerged for inverted microscopes 361 S MSC5 Microscope stage chamber 5 For coverslips 389 IE aes S PTCO3 Proportional Temperature Controller for BSC 2 087 ee S NO WWJ U S Canada prices shown International prices add 20 Email or visit web store for latest prices Ul 800 998 MATE Fixed Platform System 1 Parts Required as pictured right 8090P PSR 6 BB 5 0 RTC 0 5 RTC A3 AS 1A PH 1 Basic Platform System 2 Fixed Platform System 2 Parts Required as pictured above 8090P 1280P PSR 6 AS 1A PH 1 A Aa IN Fixed Platform System 3 Parts Required not pictured 1280P 1 AS 1A 1 BB 1 2 1 1 1 RTC 0 5 RTC A3 PH 1 60 Adapt your microscope to any application Basic Platform Systems Fixed platforms stages and shelves can be built from custom pieces or from standard configurations Many platforms begin with the model 8090P perfusion chamber holder or mounting plates The platform is held up by several spacers and support rods or towers Please contact AutoMate Scientific with your mi
56. Clamp sharp electrode or field potentials e Patch Clamp whole cell macro patch or excised patch e Voltammetry Amperometry e lon selective measurement e Bilayer recording Software Control Instead of the usual front panel knobs and switches the MultiClamp 7OOB amplifier is controlled by the MultiClamp 7OOB Commander a program that runs on a host computer and communicates with the amplifier via a USB cable This control interface provides automation of bridge balance pipette offset pipette and whole cell capacitance compensation in addition to smart features such as protection from oscillations and threshold based mode change Amplifier settings such as gain filter frequency whole cell capacitance recording mode and also input output scale factors are automatically telegraphed to the data acquisition software via messaging through the computer operating system www autom8 amp com Optional SoftPanel Controller Although the MultiClamp 7OOB amplifier is a computer controlled amplifier the mouse and or keyboard is not the only means of controlling the instrument The optional SoftPanel controller was designed for those who prefer a more conventional feel to amplifier control By way of a USB connection the SoftPanel controller physically replicates all essential amplifier functions by acting as a hardware extension of the MultiClamp 700B Commander software SoftPanel knobs replicate continuous mouse controls gliders
57. FE lined e Minimize cross contamination with BackStop back flow prevention Special 3 way relief valve design stops liquid flow quickly e Valves and pressure controls in a small box near microscope no stand necessary Includes pressurized large volume reservoir for buffer Programmable dispensing microinjection perfusion or spritzing of reproducible microliter volumes Up to eight solutions are stored in cryo tubes with easy syringe refill ports Included pneumatic valves deliver regulated air pressure to push liquids out of the SmartSquirt Perfusion Pencil tip An AutoMate Scientific or other valve controller offers programmable manual or computer controlled valve selection for switching which reagent is delivered from the tip Pulse a valve quickly for microliter delivery or leave it on for constant perfusion The short delivery path saves expensive reagents Integrated BackStop check valves prevent backflow found in competing micro perfusion systems 36 www autom8 com SmartSquirt Reservoir Block Detached SmartSquirt now standard with longer Pencil No room on your microscope stage for the SmartSquirt The Perfusion Pencil for the SmartSquirt now uses longer tubes for mounting the Reservoir Block up to 30 cm away from the delivery location The Pencil itself is also 30 cm long for easier mounting on wide stages Both standard e Uses standard 2 5 ml cryo tubes as reservoirs e Minimize cross contamination wit
58. I slot 116 Faster automated patch clamping without a microscope ChannelMAX 100A Mini and Twin ez gSEAL Pressure Controller ChannelMAX 100A Mini A system for both oocyte and cell patch clamp and two electrode voltage clamp This patent pending design is based on the PatchMAX 100A but comes with two computer controlled manipulators With the additional micromanipulator the system can do two patch clamp experiments simultaneously or one two electrode voltage clamp experiment With this new design the system increases efficiency decreases equipment cost and saves space As the system is controlled by computer experiments become less labor intensive Run two patch clamp experiments simultaneously The ChannelMAX 100A Mini comes with two computer controlled manipulators allowing users to run two patch clamp experiments simultaneously Doing two experiments at the same time allows for better decision making Single channel activities are random events It is difficult to understand what is going on by looking at the channel open and close especially when you are working on a new channel For example it is hard to www autom8 amp com distinguish a channel with two open stages from two channels in the same patch With two patches that are treated with the same conditions you have more information that could be critical in making the right decisions while working on the precious seals High quality patch clamp As in the
59. Input Impedance gt 10 GQ in parallel with 1OOpF e Two 16 bit analog outputs 740 kS s per channel Output impedance 0 2Q e 24 digital I O lines 32 bit counters digital triggering e Analog input range 10 V e Analog output range 10 V Perfusion Chamber e Accepts 1 16 tubing Computer Requirements e Windows 2000 or XP with 2 0 GHz CPU e At least 1 USB port e 1 PCI slot 118 ChannelMAX 100A Twin This system is similar to the ChannelMAX 100A Mini except it can be used for doing two two electrode voltage clamp experiments or up to four patch clamp experiments simultaneously Key Features Run dual two electrode voltage clamp or four patch experiments simultaneously With two additional micromanipulators the system can do dual two electrode voltage clamp TEVC experiments simultaneously Designed to be affordable to the scientific community this system increases productivity significantly Additional units can be run in parallel to further increase TEVC throughput As each manipulator can do one patch clamp experiment the ChannelMAX 100A Twin can run up to four patch clamp experiments at the same time This system significantly increases productivity for patch clamping High quality single channel recording Since the computer controlled manipulators guide the patch pipettes to touch the cell membrane in a more precise and consistent manner than manually controlled manipulators the success rate of making seals
60. MATE 43 Brownlee Precision Model 410 Close up view of one Brownlee Precision Model 410 channel Notice the multi use display and individual channel memory The selector knob uses an optical encoder instead of potentiometer for extremely quiet operation Gain Range 1 to 10 000 Steps 1 1 5 2 2 5 3 4 5 6 7 8 9 and factors of 10 thereof Gain Accuracy lt 2 error Lowpass Filter Range 1 kHz to 25 kHz plus wideband Steps 1 1 5 2 3 5 7 10 15 20 25 all kHz and wideband Characteristic 2 pole elliptic Wideband Frequency Response 200 kHz min all gains Input Selection A B A B or Grounded Coupling AC 1 Hz cutoff or DC Impedance 1 MQ 20 pF Bias Current 20 pA Offset Voltage 15 pV max Voltage Range 10 V CMRR gt 70 dB all gains Noise 1 5 pV 10 DV cases sed 10 kHz 44 Four channel instrumentation amplifier for clean recordings o Brownlee Precision Model 410 Amplifier The Brownlee Precision Model 410 is a four channel general purpose laboratory amplifier It can be used to amplify small signals from signal sources which may have a large common mode level and high frequency noise Each channel consists of a high gain low noise amplifier an input coupling selector a 2 pole lowpass filter and an output offset control A control knob sets the amplifier parameters and the values are displayed on a bright LED alphanumeric readout e K
61. Micro manifold stoppers set of four 16 06 21 Disposable flow regulator 20 06 21c Case of 48x disposable flow regulators 715 06 04 Disposable drippers set of 4 42 06 04c Case of 48x disposable drippers 320 01 34 Regulated perfusion reservoir gas bubbler 4 channel 312 01 35 Un regulated perfusion reservoir gas bubbler 4 channel 75 01 36 Regulated perfusion reservoir gas bubbler 8 channel 340 01 39 Un regulated perfusion reservoir gas bubbler 8 channel 115 01 40 Bubbler stones set of 4 9 50 05 11 Tygon vinyl tubing 1 16 id 0 52 t 05 13 PTFE Inert tubing 16 o d for use with nut amp ferrule fittings 3 115 4 05 14 Silicon pinch valve tubing 1 32 i d x 16 o d 2 00 1 04 09 10 Liter vacuum trap with lure lock connections and tubing 295 canister 2 Liter Perfusion vacuum trap with tubing 36 vac Lab duty oil free vacuum pump 115 VAC 599 compressor Quiet air compressor 115 VAC 220V also available 980 05 20 Deluxe Luer Kit Polypropylene approx 25 items x 10 pcs 56 05 21 Deluxe Fitting Kit Polypropylene approx 45 items x 10 pcs 52 U S Canada prices shown International prices add 15 Email or visit web store for latest prices First set of disposables included in perfusion systems Please order a minimum of 10 of tubing 800 998 MATE Quiet air compressor The EasyCode software you provided is simple to use We use a large screen Macintosh for c
62. Slides 120 Biomicroscopy Dishes 123 Biomicroscopy Accessories 124 NOILVIIGEV4 3llddidO NJIW Customer Service 126 Prices amp Payment 126 Warranty 126 Returns amp Repairs 127 800 998 MATE Perfusion System 13 01 53 ValveBank8 PTFE Inert Perfusion System Valve Controllers ValveBank Controller Reservoirs TEPE ELEI E You can choose 15 35 60 or 140ml reservoirs How to configure and order a perfusion system AutoMate Scientific sells complete perfusion systems as well as individual components This can make it confusing for customers to determine which pieces they need to order We hope this guide helps All of the items mentioned below are explained in more detail on the following pages or you can quickly customize a complete perfusion system on our web site at http www autom8 com build_your_own html 1 Gravity bath perfusion systems 2 Pressurized fast local drug application 3 Temperature control 4 Putting it all together 1 Gravity bath perfusion systems Our Perfusion Systems are complete gravity bath perfusion systems You can choose the number of channels liquids one of two valve controllers ValveBank or ValveLink and one of three different kinds of valves pinch PTFE inert and Lee valves Everything you need for a regular gravity perfusion system is included controller valves reservoirs ringstand 1 16 i d tubing and 4 8 or 16 into 1 mi
63. TUNE r tt Channel 3 2 uM Bicuculline loops Time Event 00 23 06 01 End Of List Ea Channel 6 salse l eano e ala 4 i EasyCode Software Program your ValveBank with a Macintosh or PC using easy click and drag time bars Save and load unlimited programs to disk print out program listings then download your sequences into the ValveBank s memory with the included serial cable in one simple step e Program up to sixteen channels of valves and digital outputs Open multiple experiment windows List sequences to screen or printer Copy and paste Zoom in and out of your experiment EasyCode Software Ordering Information OO2 USB iia tech O5 6 9 amp Clarsie wil USB te veal adapter 2 En 01 03 EasyCode PC ValveBank programming software Windows 3 1 95 195 01 03 2000 EasyCode PC ValveBank programming software Windows 98 XP 249 USB USB to serial converter PC or Macintosh 79 U S Canada prices shown International prices add 15 Email or visit web store for latest prices www autom8 com Precise control for whole cell or single cell superfusion oral ed ed eto eo ee E bif i I l T a io i inin ee E l i ajina fjiniipinsij inan l EE Mel lbadh agente i MAOA a E a apidgepisaetiagel ii L a a a q Bitafiepigselaene aaa a Paari ii eein I aN bigeiageidetaanel e i E COE COED TECO CELE CE ars e
64. The front panel interface of this amplifier preserves the simplicity and feel of older analog instruments while taking advantage of the benefits of digital circuitry Changing an amplifier setting is as easy as pushing a parameter button Gain for example and turning the control knob up or down until the desired setting is reached Unlike a potentiometer however the settings increment or decrement through discrete calibrated steps The bright LED alphanumeric readout displays the exact value Gain 250 for example A complete setup may be stored for each channel in memory This product is manufactured in U S A If additional features are desired such as 8 pole Bessel lowpass filter Auto Zero line notch filter highpass filter multiple memories per channel and DVM then please consider the Brownlee Precision Model 440 Brownlee Precision Model 410 Amplifier Ordering Information 04 10 Brownlee Precision 410 4 channel instrumentation amplifier 2 550 U S Canada prices shown International prices add 27 Email or visit web store for latest prices 800 998 MATE 45 Brownlee Precision Model 410 468 i aja Ley BD piima E a p n A INPUTS B 6 6 The Model 440 adds controls for a highpass filter notch filter digital volt meter auto zero and input impedance on each channel Gain Lowpass Filter and Highpass Filter generally step through the following values 1 0 11 1 2 1 3
65. ah y p e change of solution depending of course on the adjusted fluid level with mechanical stability otherwise very good and no problems with noise and the system is otherwise rather easy to set up could confidently recommend it to any investigator who works with cells that adhere strongly to the substrate Dr Jonathan E Freedman Ph D Department of Pharmaceutical Sciences Northeastern University Petri Chamber Diagram Electrode Holders Separate Inflow and Outflow Openings Petri Dish Perfusion Chamber e Perfuse cells right in your Petri dish Cells cultured in Petri dishes are a popular research tool used in applications from patch clamping to intracellular ion probe imaging True perfusion continuous inflow and outflow of solutions in the Inflow well dampens the fluctuations of the liquid l l level and prevents bubbles cells on cover slips for placement into specially designed perfusion from entering the chamber dish can be difficult to configure This forces scientists to plate chambers The PCP 1 chamber was designed by scientists after years of patch clamp research to overcome this problem Perfuse cells right in your Petri dish with any perfusion system and an optional PTFE Inert manifold sold separately Ideal for inverted microscopy using optically clear Petri dishes Adjustable metal suction tube included Dimensions 35 mm outside dia x 20 mm tall Petri Dish Perfusion Cham
66. amp standard for MultiClamp 7OOB 4 169 Mo HL U Electrode holder for all Universal U type headstages 209 Mo HS 9A x10U HS 9A headstage for Axoclamp 900A choose x0 1 x1 x10 U 779 Complete Axon CNS cellular neuroscience product line avail U S Canada prices shown International prices add 15 Email or visit web store for latest prices www autom8 com Floor standing and table top equipment racks Floor standing Equipment Rack e Standard 19 panel width 21 34 wide overall e Usable depth of 16 5 17 5 deep overall e Knockdown rack unit is shipped in a flat pack container for handling ease amp protection see above e Perfect for tight access areas or 1 person installations as unit is knocked down into 5 easy to handle parts ie 2 sides top bottom and 4 rails e Complete with 4 infinitely adjustable combination rack mount rails on E I A universal spacing 5 8 5 8 1 2 inch drilled amp tapped for 10 32 screws see close up right and 4 leveling feet e Removable gland plate over 10 long X 1 5 high opening with knockouts on rear bottom of rack for easy cable access e Simple assembly using one bolt in each corner to lock the sides to the top and bottom hardware included e Heavy duty 16 gauge steel top bottom and sides e Rails are made of 10 gauge steel zinc plated finish for grounding capabilities e Recommended maximum weight 1 000 pounds e Finished in rugged textured
67. amps Axon 200 700 Headstage Mount The MXC 45D s unique design allows repeatable electrode replacement with a simple but accurate rotating clamp The top clamp of the MXC 45D is designed to be compatible with Axon CV200B CV 7A and HEKA EPC 10 headstages The rotating portion is configured such that the probe pipette rotates up and away from the experiment at a 45 angle through a full 180 The repeatable stop returns to its location with micron level accuracy and the stop s orientation can be changed simply by loosening a single 1 4 20 cap screw Probe Clamp The MXC 45 s unique design allows repeatable electrode replacement with a simple but accurate rotating clamp The top clamp of the MXC 45 is designed to be compatible with headstage mounting rods from 3 to 10 mm in diameter The rotating portion is configured such that the probe pipette rotates up and away from the experiment at a 45 angle through a full 180 The repeatable stop returns to its location with micron level accuracy and the stop s orientation can be changed simply by loosening a single 1 4 20 cap screw www autom8 com Siskiyou Motorized Manipulator Ordering Information clamp SD MX7600L Motorized manipulator 4 axis base mount with round clamp 4 375 SD MX7600R Motorized manipulator 4 axis base mount with round clamp 4 375 SD MX7600 45DL en manipulator 4 axis base mount with dovetail 4 450 SD MX7600 45DR Motorized manipu
68. an be also measured from extracted waveforms for studying QT prolongation with the Time of Amplitude Minimum or Max to Minimum or Max measure e Averages and standard deviations for the beat frequencies inter spike intervals and waveform analyses on all 64 channels can be automatically computed and graphed for each user defined phase of an experiment Dose response curves can thus be easily constructed Data Output Raw data can be exported in binary or ASCII text file csv format Extracted spikes and all measurement charts can be saved as csv formatted text files 112 Mobius QT Mobius QT software allows MED64 users to acquire and analyze myocardial signals online and offline It is the perfect solution for QT screening with iPS ES cell derived cardiomyocytes Ren TS oe PT aij ae Lawes Eisi Slama i j hammi E z Beat frequency and inter beat interval are measured Myocardial signals are detected highlighted in bright green E F E i eles i Har iie jap DI maeri daman hare Deeg Las Fl es pase te es i H i EF 1 Tm nsw F l I i ee m S a a i eee ee Leb eam The time between peaks i e QT interval is measured and plotted over time oo i ui j i 1E aa Pours A dose response curve is generated www autom8 com Perfusion is our specialty Keep your cells happy
69. and manufacturing electro physiology equipment combined with partnerships with the most reputable Valve Controller equipment manufacturers AutoMate Scientific is in a unique position to Hum Bug provide you with all of the equipment to build or upgrade your electro capers Cental physiology workstation From Axon Instruments MDC amplifiers digitizers and software Sutter Instrument microelectrode pullers micromanipulators and imaging products to TMC air tables we can provide a turn key rig delivered to your door or just a few pieces Our prices are competitive but you will keep coming back for our personalized customer service Assemble a complete rig online with our new graphic web form at http www autom8 com rig Contact our sales scientist at AutoMate Scientific to get a quote today Put our experience to work combining all of the hardware and software you need to record and publish We offer configurations for patch clamping Instrumentation Amp extracellular and intracellular microelectrode recording Xenopus oocytes and multielectrode array systems Patch Clamp Amplifier Data Acquisition Electrophysiology Rig Ordering Information TMC Air Table and Faraday Cage MDC AXON INSTRUMENTS MultiClamp MultiClamp 700B amplifier 17 629 Digidata Digidata 1550A 7 539 pClamp pClamp 10 Software for Windows 6 029 SoftPanel SoftPanel 2m9 BROWNLEE PRECISION Model 410 instrumentation amplifier 2 550
70. aneously thanks to the microprocessor design e Low cost amp low profile simple design Optional BNC cables and 19 rack mounting brackets 800 998 MATE 15 ValveBank4 amp 8 e Dimensions 10 x 7 5 x 2 Weight 5 Ibs 2 27 kg e User selectable for normally open or closed valves e 1 5 amp 12V AC power supply included e Back lit LCD display e Detached 16 key membrane keypad e Easy menu driven interface e 16 or 32 user programs of 256 commands up to 99 hours long EasyCode Software e Program up to sixteen channels of valves and digital outputs e Open multiple experiment windows List sequences to screen or printer Copy and paste Zoom in and out of your experiment Please see page 20 16 All AutoMate Scientific valve controllers switch 12V DC solenoid valves open and closed rapidly using full power then hold in at 2 power to prevent thermal transfer to your solutions Low noise circuitry minimizes recording artifacts in electrophysiology ValveBanks are designed for use with pClamp Pulse et al All AutoMate Scientific products include a one year warranty The ValveBank remains the only programmable valve controller for physiology that does not require a computer ValveBanks include digital and manual control plus programming through their keypad and LCD screen or EasyCode software from a Macintosh or PC ValveBanks run user valve sequences without a comp
71. are coverslip slip reservoir 2 places Unheated perfusion chambers horizontal round and vertical QS U H V amp R These use 22 mm square 1 coverslips on the bottom and accept 13 mm round coverslips on top These work with any of the stage adapters to the left 35mm Petri Dish Holders and Heaters Unheated 35mm Petri dish holder QS U 35 Heated 35mm Petri dish holder QS H 35 with spring clips and Nunc adapter ring with spring clips and Corning adapter ring This adapter also holds full size slides www autom8 com Microscope Stage Adapters Olympus Narishige Scientifica 110 mm O Nikon Siskiyou 108 mm with locking tabs N Zeiss Leica M ZM Zeiss Leica K ASI Marzhauser Prior Sutter MT MP 78 ZK Retainers Tool Trays are ferro magnetic stainless steel plates designed to hold your Universal Adapter UU Leveling screws front and rear Flexible stainless steel mounting plate See chart on the next three pages to select the appropriate stage adapter for your microscope and stage If you don t have room on your stage for a Tool Tray or think it is too perfusion chamber down to the stage adapter while also providing a large large you can order your QuickStage with Clamps instead They surface for StageHands magnetic tool holders Round stage adapters i e hold the perfusion chamber down to the stage adapter using the Olympus and Nikon include a square Tool Tray 4 25 in 10 8
72. as easy as pushing a parameter button Gain for example and turning the control knob up or down until reaching the desired setting Unlike a potentiometer however the settings increment or decrement through discrete calibrated steps The bright LED alphanumeric readout displays the exact value Gain 850 for example Multiple setups may be stored for each channel in memory All the input and output BNC connectors are conveniently located on the front panel Each channel functions independently and is internally shielded to prevent crosstalk If the input signal has a drifting DC offset voltage it can be removed using the Highpass Filter The Output Offset control can shift the output baseline level from 10 V to 10 V in 100 mV steps This is helpful if the output level must match the input range of a computer s A D board for example The Model 440 s powerful output stage can drive 100 mA and is stable even driving capacitive loads A notch filter is available to remove line noise Brownlee Precision Model 410 Amplifier Ordering Information 04 40 BP 440 High performance 4 channel amplifier and signal conditioner 3 950 U S Canada prices shown International prices add 20 Email or visit web store for latest prices 800 998 MATE Highpass Filter AC Coupling Range 01 Hz to 100 Hz Steps DC AutoZero DC then as above Input Selection A B A B or Grounded Impedance 1 MQ or 10 Q 20 pF Bias Current 20
73. base of the table legs TMC Air Table Ordering Information TM 63 7590 63 Series CleanBench High Performance Lab Air Tables 4 420 TM 81 301 012 Front support bar 63 500 Series 185 TM 81 302 012 Rear support bar 63 500 Series 185 TM 90 510 Ontrak pre assembled roll off feature 415 compressor Quiet air compressor 980 Complete TMC product line available Call or email for part numbers U S Canada prices shown International prices add 15 Email or visit web store for latest prices Breadboards Another in a long line of TMC innovations the 75 Series Breadboards minimize both weight and cost A patented manufacturing technique allows us to form countersunk tapped holes in a thin top skin of stainless steel The holes are not drilled or punched but fabricated in a way that effectively thickens the skin in a small ring around the threads No inserts are used and the tapped holes are 3 threads deep These tops have relatively high levels of rigidity as they utilize our standard steel core They are ideal when light loads are anticipated and low weight and or cost are the most crucial factors This lightweight design is available from stock in several sizes A full range of sizes and materials is available on a custom basis e Ultra lightweight design weighs less than 9 lbs ft2 44 kg m2 e Ferromagnetic Stainless steel top and bottom skins e Aluminum and carbon steel versions availabl
74. ber Ordering Information Petri dish perfusion chamber insert optional specify Corning PCP 1 195 Falcon Nunc Micro manifold also recommended U S Canada prices shown International prices add 15 Email or visit web store for latest prices 800 998 MATE 41 m Z Y T m z5 Ze E 4 Z R I gt EA es m A can perfuse up to five animals an hour plus if costs no more than a tube of enzyme Dr Yien Ming Kuo University of California San Francisco Mouse Brain Striatal Section Easy switch from saline to fixative just turn the stopcock Dr Sharon Smart University of California San Francisco 2 way Manual Stopcock Easy one handed operation to switch solutions 42 We ve built a great fixation system so you dont have to In Vivo Manual Gravity Perfusion Systems Rat 800 ml 91 cm tall and mouse 140 ml 61 cm tall systems shown e Consistent results Steady gravity fed delivery of buffer and fixative results in complete perfusion e Easy to setup use and clean Complete system with everything down to the needles Fits in standard hoods e Fraction of the cost of a peristaltic pump and more reliable AutoMate Scientific is providing an option for the scientist who would rather do science than design a system source parts phone buy etc Let us take the guesswork out of your new animal fixation system One or two users can perfuse two mice
75. ble with 35 or 60 ml syringes and rubber stoppers shown in this photo or 50 ml screw cap reservoirs 21 ae 28 m WA vA Zu N m Oo WY U m zal TI Y O Z Zz U Li Oo LLI A D v am LLI N a D U Va LLI A Precision Components e Stainless steel construction Kinle te mk e 5 micron filter and gauge e Precision regulator delivers O 15 psi e Convenient toggle switch completely closes gas connection to tank when not in use Oxygen8 Bubbled Pressure Kit You need rapid liquid switching with oxygenated solutions How do you bubble Carbogen gas into closed pressurized reservoirs That s the engineering problem AutoMate Scientific has solved with several years of research bubbled pressurized perfusion You can add the new Oxygen8 to any new or existing gravity perfusion system from AutoMate Scientific any third party manufacturer or even homemade rigs Connect to your desired gas 30 to 100 psi Available in four or eight channel configurations Syringe reservoirs may be placed in a water bath for temperature control or combine with the AutoMate BubbleStop syringe heater Pressurized Superfusion Ordering Information 09 04 Perfusion Pressure Upgrade 4 channel 395 09 08 Perfusion Pressure Upgrade 8 channel 495 09 16 Perfusion Pressure Upgrade 16 channel 750 with 35 or 60 ml plastic syringes filter regulator and manifolds 09 04n Per
76. bs 160kg Series 64 TableTop Platforms Finish Effectively isolates floor vibration with unprecedented ease portability and Medium texture black powder economy coat frame stainless steel top or granite top Features e Gimbal Piston Isolators Facilities required 80 psi nitrogen or air e Top plates available in granite stainless steel laminate and CleanTop I Number of Gimbal Piston Isolators Required For granite tops and CleanTop II e Attaches to tops up to 2 1 8 thick Isolators cradle platform for stability Beadad eeoa steel honeycomb breadboard adequate for most loads Some e lsolator housings protect isolators from being disturbed applications may benefit trom a fourth isolator to allow alternate isolator arrangements The effect on isolation is negligible Heavier TMC Air Table Ordering Information stainless steel laminate tops generally use four isolators for better PartNo Product Description Price 70 00eeon TM 64 446 64 Series TableTop Platforms 3 285 Complete TMC product line available Call or email for part numbers U S Canada prices shown International prices add 15 Email or visit web store for latest prices 800 998 MATE 99 O O N ae Lit N l Y 2 aa O Li lt L QA Q O Lit mM lt L General Specifications Net Load Capacity 150 Ibs 67 kg Facilities Required 80 psi air or nitrogen Finish Electropolis
77. complex induction sequences from any of the two built in stimulation units Fast easy switching of simulation sites MED64 Connector Interfaces between the MED64 probe and amplifier Provides a stable platform for the MED64 probe Multi layer electrical shielding rejects hum noise and provides excellent signal to noise ratios No onboard active circuitry enabling long term recordings in a humidified incubator Heated version available with built in thermocouple which maintains bath temperature within 1 C usually lt 0 1 C using a low noise ThermoClamp controller with or without perfusion Comparison of Electrode Impedance Our proprietary platinum black coating process gives the MED64 probes significantly lower impedance green line 20 um square electrodes and red line 50 um square electrodes in graph to the right than competing electrodes purple line 30 um round electrodes This lower impedance means Better signal to noise ratio Smaller stimulus artifacts Higher current stimulation e g 200 pA can also be applied No pre amplifier required Connector can be used with long cables and placed in an incubator for long term studies Easy to acquire signals even from acute slices without unnecessary 3D electrodes or perforated dishes 800 998 MATE Connector Specifications Complete line of specialized perfusion equipment available Impedance KQ Now available heated Probe securing mecha
78. cro manifold with flow regulator Gravity PTFE inert systems include drippers and a reservoir bracket This Perfusion System is ideal for switching solutions flowing into a perfusion chamber for slice electrophysiology oocytes imaging etc with flow rates from near zero up to 6 10 ml minute We usually recommend pinch valves cheapest and easiest to clean unless you need faster switching Our Gravity Perfusion Systems Ordering Information 17 pp 54 ValveLink8 Pinch Valve Perfusion System 2x 01 05 Low noise grounding packages 4 valves each 01 20 Full BNC cable or alternate cable for Heka amplifiers 13 pp 54 ValveBank 8 Pinch Valve Perfusion System You can choose 15 35 60 or 140ml reservoirs www autom8 com ValveLink controller is designed for customers who have digital TTL outputs from their computer from data acquisition software like pCLAMP Pulse Patchmaster WinWCP MetaMorph etc or I O hardware with software like LabView or MATLAB Alternatively our ValveBank controller is completely programmable without needing a computer It is ideal for setups without computer control or imaging software with only 1 2 digital trigger or shutter outputs 2 Pressurized fast local drug application Perfusion Pencil For customers needing faster switching times ligand gated channels etc we recommend local drug application often called spritzing or superfusion This means the perfusi
79. croscope model and application patch clamping multi site patch clamping blind patch or extracellular work etc and we can configure an economical platform for your inverted or upright microscopes www autom8 com Manual and moforized microscope mounting Manual amp Motorized Microscope Platform The MXZP platform system can be custom tailored to suit your needs The platform configuration shown is just one of many that Siskiyou can produce The system is supported by our 100cr series stages crossed roller They are available in manual or motorized versions A full 25mm of travel is provided on both models The manual versions can be fitted with either 20 40 80 or 100 TPI adjustment screws with resolution from 20 um down to 5 um respectively Please specify which pitch of adjustment screw you require when ordering Replace xx in the Model Number with the desired thread Motorized versions can be controlled with any e series and MC2000 controllers Our controllers drive the stage through a closed loop interface between the controller and the motor encoder The closed loop connection ensures 0 2 um and 0 1 um resolution respectively The encoder coupling also enables the use of the DR1000 digital readout for repeated or relative positioning requirements All cables are shielded to ensure noise free operation during sensitive electrophysiology experiments 800 998 MATE 61 Features e 2 5 to 12 0 inch ve
80. d HS 2A unity gain headstages The HS 2 and HS 2A headstages are used with Axoclamp 2 and GeneClamp amplifiers They are all unity gain voltage recording headstages but they come in a variety of different current passing gains for applications as diverse as extracellular recording bath potential recording ion sensitive recording ionophoresis and intracellular recording from small or large cells For ultra high impedance electrodes special circuitry inside the headstage prevents any DC current from leaking into the input through the capacitance neutralization circuit HS 4 x1MGU relay switched unity gain headstage HS 4 headstages may be used with Axoclamp 2 amplifiers to maximize the voltage across the electrode during two electrode voltage clamp In all other modes the HS 4 acts like an HS 2 headstage Available only with current passing gain xIMG The VG 2 headstage must be used for Current measurement www autom8 com VG 2 virtual ground headstage The VG 2 virtual ground headstage may be optionally used with Axoclamp 2 amplifiers to measure whole bath current Standard current measurement gains are x0 1 x1 and x10 x100 is also available Current recording ranges for these virtual ground headstages are 0 1 pA x0 1 1 pA x1 10 pA x10 100 pA x100 VG 2A x100 bath clamp headstage The VG 2A x100 bath clamp headstage may optionally be used with Axoclamp 2 or GeneClamp amplifiers to clamp the bath potential at zero volts
81. dead volume Usable aperture of dish limited by the opening in heat transfer plate Surface evaporation significantly contributes to non linearity of temperature distribution Advantages of the Delta T Dish System e Place cells onto coverglass and observe e Highly accurate temperature control Fast thermal recovery Superior optical image Stage adapters to fit most popular microscopes Designed for inverted microscopes but ideal for water immersion objectives on uprights Immediate alarm if cell temperature changes e Rigid mount for X Y stability Uniform temperature distribution Cells unaffected by surface evaporation e Numerous specimen adapters available 102 The Bioptechs Inc Delta T Culture Dish System is designed to simulate host conditions on the stage of your microscope and provide an optimal optical environment for microscopy This two step system allows you to plate your cells and observe them without having to transfer them to another structure The system components are the Controller Stage Adapter and Dishes Accessories for Tissue Slice Brain Slice and other specimens are available An intelligent feedback loop passes an electrical current through a thin film coating on the underside surface of the glass substrate on which the cells are grown Heat is applied directly to the cells without the inefficiencies associated with peripheral heating by traditional culture dish warmers Biop
82. der paint finish e Requires mounting hardware 92 Table Top Equipment Rack Features e Standard 19 rack panel width mounting e Rack mounting on E I A universal spacing 5 8 5 8 1 2 inch e Round hole punched for 10 32 clip nuts included e Choice of two heights 21 12U or 28 16U e Heavy duty construction 14 gauge steel e Supplied unassembled four pieces shipped in a flat pack container for handling ease amp protection e Includes all hardware and four rubber feet e Recommended maximum weight RRTT1928 tested 350 pounds e Rugged powder paint textured black finish 2 075 Equipment Racks and Shelves FR TT21 Table top rack short 21 12U tall x 19 wide rack 150 FR TT28 Table top rack tall 28 16U tall x 19 wide rack 161 FR TT Shelf Table top Rack Shelf 7 deep x 19 wide rack x 1 75 tall 48 FR EQ35 Equipment Rack 35 20 U tall x 19 wide rack x 16 5 deep 460 FR EQ42 Equipment Rack 42 24 U tall x 19 wide rack x 16 5 deep 495 FR EQ63 Equipment Rack 63 36 U tall x 19 wide rack x 16 5 deep 661 FR EQ Shelf Equipment Rack Shelf 15 deep x 19 wide rack x 3 47 tall 68 FR 1425PHD Equipment rack casters Heavy duty 600 lbs set of 4 77 FR 1425PL Equipment rack casters Light duty 400 lbs set of 4 39 More sizes and accessories available Contact AutoMate U S Canada pric
83. ders e Power off recording mode for zero electrical noise e Flip slide rotation mechanisms for easy electrode exchange e Plug and play installation of up to 14 micromanipulators Designed to meet your needs High positioning resolution provides a precise approach for high quality seals in patch clamp experiments A distinct penetration mode offers high acceleration piezo thrusts for clean cell impalements in sharp recordings The small size enables easy installation and use in space constrained imaging and multiple micromanipulator systems Electrode exchange using memory positions and quick electrode retrieve return mechanisms is as convenient as it gets Sensapex micromanipulators will make your experiments easier and more successfull Sensapex Piezo Micromanipulator Ordering Information SE SMX 1R_F_XXX Micromanipulator system with bolt mounted stand 8 215 SE SMX 2_F_XXX SE SM 1R_F Dual micromanipulator system with bolt mounted stand 13 038 4 823 Right handed micromanipulator ONLY with stand 800 998 MATE Specifications Micromanipulator Positioning range 20x20x20 mm x y z Step resolution 30 nm Max speed 5 mm s Max load 70 g or higher True approach angle 0 50 or 40 90 degrees Dimensions 39 x 82 x 75 mm Weight 260 g Controller Rotary optical encoders and backlit display Six speed settings impalement Programmable Home and target positions Batteries Li ion
84. does not create phase delays amplitude errors DC shifts or waveform distortion Simply connect it between your preamplifier and any analysis equipment and it will automatically eliminate 50 60 Hz noise and harmonics with frequencies up to several kHz Noise is eliminated without altering the signal of interest even when frequencies within the signal overlap with noise components No settings or adjustments are required The Hum Bug can eliminate 50 60 Hz noise from virtually any analog signal It is equally effective at removing noise associated with inadequate grounding ground loops and electrical pickup Common applications include noise elimination from signals recorded using microelectrodes skin electrodes EKG EMG EEG high gain amplifiers magnetic sensors and audio equipment Hum Bug Noise Eliminator Ordering Information Q HUMBUG Hum Bug 50 60Hz active noise eliminator 1 725 U S Canada prices shown International prices add 15 Email or visit web store for latest prices www autom8 com Deliver precise constant volfage or current stimuli DS2A Constant Voltage DS3 Constant Current DS4 Bi phasic Stimulus Isolators Brief pulses of electricity are used in various biomedical research applications as a stimulus to excite nerve or muscle fibers Several factors need to be considered when choosing the right stimulator e In order to minimize artifacts introduced into electrophysiological data it is d
85. e e Formed holes are countersunk and tapped 3 threads e Formed holes in top skin add to rigidity e Cleanlop individual cups under each hole make the core spillproof TMC Breadboard Ordering Information TM 75SSC 103 02 Breadboard 19 x 23 75 Series metric sizes with M6 holes 805 TM 75SSC 104 12 Breadboard 19 x 47 75 Series available 1 050 TM 75SSC 115 12 Breadboard 23 x 35 75 Series 1 040 TM 75SSC 135 02 Breadboard 35 x 47 75 Series 1 560 Complete TMC product line available Call or email U S Canada prices shown International prices add 15 Email or visit web store for latest prices www autom8 com Industry standard electrical Until recently TMC s Faraday Cages e e have only been available as part noise D rotecti ON of a complete vibration isolation table system The cages required our Perimeter Enclosure option and could not be assembled without a TMC 63 500 Series Vibration Isolation Table as its base We now offer the same line of AO in tall cages with a baseplate which allows the cage to be used on a bench top without a corresponding TMC table The base of the cage is a reinforced stainless steel plate which can support a compact vibration isolation system microscope or other instrument Options and Features Type Il Cages e New 2 in diameter holes for cable passage Optional armrest pads are now available with Faraday Cages Optional U shaped
86. ed imaging of living or fixed cells High throughput screening HTS in cell culture Compound screenings toxicology Large scale transfection experiments Compatible with automation equipment Standard format and dimensions of 85 5 x 127 5 mm 96 384 quadratic wells with standard numbering Excellent inner well flatness and whole plate flatness www autom8 com Biomicroscopy j Dishes Ask for 3 free samples sh 35mm with high or low walls or glass bottom 50mm amp grid versions e Standard sized 35 mm Petri dish with a thin bottom for high end microscopy and cell based assays e Low wall version for micromanipulation and microinjection e Fluorescence microscopy of living and fixed cells e Now available with DIC lids and XY grids e Glass bottom version for TIRF and single molecule applications e 50 mm size with large cultivation area also available ture Inserts for wound healing 2D invasion assays stem cells e Co cultivation 2D invasion assays e Preparation of two individual cell patches or coatings e Sticky on underside no material remaining when removed e Defined cell free gap of approx 500 um e Tested for cell compatibility made of silicone e A well stem cell Culture Inserts for differentiation also available 800 998 MATE 123 Od 7 7 O TA om oO vV lt ii U aE m U Slide and p Dish Accessories VY ad O v U Li LJ LJ lt L gt Q
87. ee decades It has two BNC output sockets i the SYNC output produces a pulse to trigger recording devices or synchronize other equipment and ii the OUT output produces either a delayed version of the same or pairs of delayed and non delayed pulses 800 998 MATE Additional Specifications Delay Total range 1 ms 1200 ms in three overlapping ranges Control Single turn control marked 1 12 ms with intermediate integer panel marks Accuracy 1 at 1 and 12 marks 5 at intermediate marks Multiplier x1 x10 x100 Internal Jumper Enable x10 giving a 10 ms 12 sec total range The front panel provides a check box for marking with a waterproof pen when enabled Indicator TOO LONG Red LED that flashes if DELAY is longer than can be produced for each pulse 55 Outputs SYNC Connector BNC socket Signal Positive going 200us pulse TTL compatible pulse 5V amplitude Outputs OUT Connector BNC socket Signal Positive going 200 us pulse TTL compatible pulse 5 V amplitude Control Selection of only the Delayed pulse upwards or both the Sync and Delayed pulses Indicator Amber LED flashes for each OUT output pulse Internal Jumper Enable Active Low giving an Output that is Low during its active phase The front panel provides a check box to mark with a waterproof pen when enabled Power Consumption lt 2 mA Internal 9V PP3 IEC 6LR61 style Alkali
88. eed settings rapid 1 7 mm second and medium 300 um second The third speed selector slow has a variable 330 potentiometer that enables settings from high speed 50 um second to low speed 2 um second With the speed selector set at the slowest settings consistent 0 2 um moves are easily made by the simple bump of an axis button Our easy to use ABDC switching box allows control of one to four 4 axis micromanipulators by one controller MC2000 excluded The basis of the design is simple and requires only an extra controller junction box for each added micromanipulator or motor drive group up to four per group Junction boxes must be purchased separately however this is more cost effective than individual handheld control units When used with the MC1100e controller the user can save a target location for each manipulator independent of the operation of the others even after switching between micromanipulators The switch box comes with enough cables to attach four junction boxes and all connections are shielded 800 998 MATE SYHOLV INdINVW AQATINSIS SISKIYOU MANIPULATORS Features e Water based hydraulic system e Less than 2 um drift hour e Ideal for intracellular recording Performance Specifications e Travel axis Total 0 80 inch 20 mm Fine 0 2 inch 5 mm e Minimum controllable motion Coarse 10 um Fine 0 5 um Features e Unique swing out design e Spring
89. em 2 395 13 pp 24 ValveBank4 Pinch Valve Perfusion System 1 32 i d silicone tube 1 895 13 21 27 ValveBank4 Lee Mini Valve Perfusion System 2 595 17 01 23 ValveLink4 2 PTFE Inert Perfusion System 2 295 17 pp 24 ValveLink4 2 Pinch Valve Perfusion System 1 32 i d silicone tube 1 795 17 21 27 ValveLink4 2 Lee Mini Valve 1 5 to 4 ms Perfusion System 2 495 13 01 53 ValveBank8 PTFE Inert Perfusion System 3 995 13 pp 54 ValveBank8 Pinch Valve Perfusion System 1 32 i d silicone tube 3 195 13 21 57 ValveBank8 Lee Mini Valve Perfusion System 4 385 17 01 53 ValveLink8 2 PTFE Inert Perfusion System 3 295 17 pp 54 ValveLink8 2 Pinch Valve Perfusion System 1 32 i d silicone tube 2 495 17 21 57 ValveLink8 2 Lee Mini Valve 1 5 to 4 ms Perfusion System 3 685 17 01 83 ValveLink16 2 PTFE Inert Perfusion System 5 995 17 pp 84 ValveLink16 2 Pinch Valve Perfusion System 1 32 i d silicone tube 4 695 17 11 87 ValveLink16 2 Lee Mini Valve 1 5 to 4 ms Perfusion System 7 995 U S Canada prices shown International prices add 15 Email or visit web store for latest prices xx TB xx Indicate Tlop inflow and Blottom outflow PTFE Inert valve fittings 0 1 8 i d hose barb 1 1 16 i d hose barb 2 Luer lock female with stopcocks and 35 ml syringes 3 10 32 threaded nut amp ferrules for 1 16 0 d tubing add 30 set of 4 p Pinch valves have no fittings Systems include Controller user
90. en be counted converted to frequency or further analyzed using other modules Use of the NL120S AUDIO AMPLIFIER and NL985S LOUDSPEAKER would allow the spikes to be monitored audibly Intracellular DC Recording The NL102G DC PREAMPLIFIER is a suitable amplifier for intracellular recording it can also be used for extracellular micro electrode recordings Used with the NL106 AC DC AMPLIFIER it provides a total gain up to x1 000 while the NL125 6 FILTERS give continuously adjustable bandpass and notch filtering from DC to gt 50 kHz The NL102G features capacitance neutralization current injection up to 100 nA and impedance checking Internally generated current injection and impedance checking can both be controlled by other devices or NeuroLog modules The NL102G now includes the NL412 PULSE box allowing remote and full activation of the capacitance compensation circuitry This buzz process can aid penetration during electrode impalement ur Channel Isolated Amplification for EEG EMG or ECG Recording An ideal system for multi channel AC recording of physiological signals such as EEG EMG or ECG in the research environment The system provides a wide range of amplification and filter settings The NL844 4 CHANNEL PREAMPLIFIER can be positioned near the recording site reducing the length of the electrode cables and minimizing interference The outputs are connected to the NL820A ISOLATOR housed in the Neur
91. erform the competition It achieves both horizontal and vertical isolation down to very low input levels e Thin Wall Rolling Diaphragms An integral part of the Gimbal Piston the thin wall dacron reinforced rolling diaphragm air seals are only 0 020 in 0 5 mm thick and extremely flexible They do not stiffen the spring as thicker rubber diaphragms do 800 998 MATE 23 CE a M O VY Li mal lt L gt aa O lt L O D lt L 94 e All Aluminum Height Control Valves All systems are equipped with rugged all aluminum height control valves Virtually unbreakable they are finger adjustable with no need for tools The standard model maintains height to 0 050 in 1 mm the precision model to 0 005 in 0 1 mm Internal Piston Travel Restraint Unique in the industry Technical Manufacturing Corp provides husky tamperproof built in piston travel restraints The restraints are completely independent of the table valves and have been ram tested at forces above those produced by the pistons operating at full pressure They cannot be decoupled accidentally and do not interfere with setting up and using the table but simply protect against overtravel without the use of external bars that create hazardous pinch points Heavy loads including the top plate can be safely removed from a table in full operation Tiebar Gussets Exclusive TMC tiebar gussets increase table frame rigidity They com
92. ertical 0 5 Hz actively suppressed Horizontal 0 5 Hz Isolation efficiency 2 Hz Vertical 10dB Horizontal 20 dB Net Load Capacity 1 200 Ibs 545 kg Finish Medium texture black powder coat frame stainless steel top Facilities required 3 CFM 80 psi air filtered to 20 microns or less 120 VAC or 15 VDC 7 w nom 12 w max Features Compact Sub Hertz Pendulum Isolation System CSP for 0 5 Hz horizontal resonant frequency e PEPS Precision Electronic Positioning System for non contacting height control of isolated surface to one micron e PEPS VX Vibration Cancellation add on to PEPS Precision Electronic Positioning System for low frequency vibration cancellation in the three vertical degrees of freedom e Rigid steel frame construction in a desk style configuration e Highly damped high stiffness two inch thick stainless steel laminate top Applications e Atomic Force Microscopes e Scanning Probe Microscopes Commercial Interferometers e Electrophysiology Recording e Semiconductor Inspection Equipment 98 State of the art vibration isolation performance 20 SERIES Active Vibration Isolation Tables TMC s Active Vibration Isolation Table features state of the art vibration isolation performance By integrating their new Compact Sub Hertz Pendulum Isolation System CSP for horizontal vibration reduction with the new PEPS VX Inertial Damper for vertical vibra
93. ervoirs Custom closed pressurized Nalgene HDPE reservoir bottles are available in 1 2 and 10 liter sizes with ringstand holders Why keep refilling buffer all day These require an AutoMate Scientific Pressurized Perfusion System or regulated pressure source 800 998 MATE 4 8 and 16 into 1 PTFE Inert micro manifolds for bath perfusion combine multiple tubes 1 16 1 6 mm i d or o d into a single outflow tube The manifolds include a built in flow adjustment screw to decrease flow rate without adding dead volume The 1 4 inch 6 35 mm outflow is interchangeable with manifolds from Warner Instruments and all AutoMate Scientific perfusion chambers PTFE Inert Stoppers are available to temporarily close unneeded holes spital I V perfusion accessories Disposable hospital V drippers drip chambers get flow started in empty tubes and help visualize flow rates Flow regulators restrict perfusion flow rates like the adjustment screw in our micro manifolds They are helpful in matching perfusion inflow rate to outflow rate Both use 1 8 i d tubing Case pricing available Perfusion reservoir gas bubbler Available with or without an oxygen safe regulator the Perfusion reservoir gas bubbler is used to split a gas bottle like oxygen carbogen or CO into multiple perfusion reservoirs The included high quality stones create a stream of fine bubbles to evenly saturate your solut
94. es for Fast Switching Required for fast kinetics applications Excellent chemical and corrosion resistance Non stick surface resists particles and chemical deposits Switches in less than 10 ms with 20 ul of dead volume from port to port Threaded female inlet and outlet ports accept Hose Barb Luer Lock and Nut amp Ferrule fittings see diagrams next page e Lee Mini Valves for Extremely Fast Switching and Minimal Pressure Pulse For the most demanding applications AutoMate Scientific offers tiny valves from the Lee Company Enclosed in our new aluminum box with luer locks for syringe reservoirs these valves can open and close in 1 5 4 ms with a ValveLink8 2 controller Perfusion Systems Include Controller valves 6O ml syringe reservoirs 2 way stopcocks reservoir bracket and drippers in PTFE Inert systems only ringstand 1 16 i d tubing and four eight or sixteen into one micro manifold with built in flow control 5 15 35 60 or 140 ml syringe reservoirs available The Economy Pinch Valve System includes a ValveLink8 controller four pinch valves 35 ml syringes 2 way stopcocks ringstand 1 16 i d tubing and four into one micro manifold with built in flow control Computer Interfacing Perfusion systems can be controlled by a computer using data acquisition hardware i e DigiData ITC 16 or National Instruments board and software i e oCLAMP Pulse or LabView Both ValveBanks and ValveLinks accept real t
95. es shown International prices add 15 Email or visit web store for latest prices www autom8 com No shake rattle or roll igh Input Speciation Low Input Specifications Vertical 1 5 2 0 Hz Horizontal 1 2 1 7 Hz Isolation Efficiency 5 Hz Vertical 70 85 Horizontal 75 90 gt us OQ JJ gt Q JJ lt gt us m Y On T UI Isolation Efficiency 10 Hz Vertical 9O 97 Horizontal 90 97 Gross Load Capacity 1 400 Ibs 640kg Net Load Capacity 350 Ibs 160kg Finish Medium texture black powder coat frame stainless steel top Facilities Required 80 psi nitrogen or air Series 63 CleanBench High Performance Laboratory Tables Accessories e Front Support Bar TMC 63 Series CleanBench High Performance Lab Tables provide an e Rear Support Bar excellent vibration free working surface for loads up to 350 Ib 160 kg e Armrest Pads e Sliding Shelves Articulated ArmRest e Raised Rear Shelf Now with modular construction these tables are recommended for use in such diverse applications as electrophysiology cell injection ultramicrotomy photomicroscopy scanning tunnel microscopy and confocal laser lt Sub Shelf scanning microscopy e Clear Acrylic Enclosures e Fixed Full Perimeter Enclosures e Gimbal Piston Isolators e Precision Control Valves e Casters TMC s Patented Gimbal Piston Isolator has been proven by independent tests to consistently outp
96. esirable to electrically isolated the stimulator from both ground and the trigger device e The voltage required to send current through tissues can vary greatly making it important to have control over the stimulus driving force e Large impedance variations during an experiment can result in loss of the stimulus In this event a constant current stimulator may be more suitable Our three isolated stimulators either provide constant voltage DS2A constant current DS3 or bi phasic output DS4 giving you the ability to choose the stimulator which best suits your experimental needs 800 998 MATE DS2A Constant Voltage Stimulator External control of pulse duration Overload protection circuit preventing current in excess of 50 mA being delivered DS3 Constant Current Stimulator Four current ranges allow precise control of output between 2 pA and 32 mA Output discharge Clamp circuit prevents capacitance build up during stimulus trains which is important to prevent stimulus loss 90 V compliance provided DS2A amp DS3 Features Accurate and reproducible stimulus characteristics Switchable polarity variable output and duration ranges 20 us to 2 sec External pulse duration control through the BNC trigger input A single shot button which operates irrespective of trigger inputs Cases manufactured from insulating material may be rack mounted using an optional mounting frame Model D121 11 ava
97. etup stimulation parameters drug testing data recording and analysis Low noise and High S N recording e Very low impedance planar microelectrodes typ 7 10 kQ at 1 kHz the lowest on the market means the MED64 system is relatively unaffected by exogenous noise and does not usually require a Faraday cage e High quality signals can be acquired easily even from acute slices The low impedance electrodes result in very low Johnson noise www autom8 amp com Effective stimulation The planar microelectrodes coated with platinum black have tremendously high capacitance values enabling high frequency stimulation with large current amplitudes up to 200 pA 0 2 msec Stimulus artifacts have very short durations so high quality electrically evoked signals can be recorded soon after without interference Long term observation without damage to tissue Dissociated cells slices and explants can be cultured directly on the probe The MED probe and connector can be placed in an incubator with 100 humidity Responses can be recorded for days to months without risking damage to tissue by the planar microelectrodes Components MED64 Integrated Amplifier Reliable low noise 64 channel amplifier High bandwidth of 0 1 Hz 10 kHz allows users to record several types of extracellular potentials Samples at 20 kHz ch Built in stimulator allows users to study the effects of realistic spatio tem poral stimulation patterns by programming
98. ey Features Clear front panel controls for all settings Calibrated gains from 1 to 10 000 Single ended or differential inputs 2 pole elliptic lowpass filter with range 1 kHz to 25 kHz AC or DC input coupling Low noise circuitry Self trimming feature to remove internal offsets Memory on each channel Powerful output which can directly drive transducers Wide bandwidth greater than 200 kHz on all gains www autom8 com The Brownlee Precision Model 410 is a rugged precise and easy to use Output laboratory amplifier It is well suited to a wide variety of research and test Voltage Range 10 V applications particularly in the electrophysiology field Crenit Capability F100 mh Slew Rate 10 V uS Each of the four amplifiers has a non inverting A and an inverting Output Ofiset Cone Renge B input with front panel BNC connectors The inputs can be switched 210 Vin No to be DC coupled AC coupled or grounded The differential or single Noise 500 pV ended signal is amplified by the selected gain and high frequency noise Gi ja 10 kHz is rejected by the adjustable lowpass filter The output level may be shifted by as much as 10 Volts with the output offset control The Model 410 s input voltage noise is only 1 5 pVim or 10 UV ocak to peak measurement bandwidth of 10 kHz Each channel functions independently and is internally shielded to prevent crosstalk The output is capable of driving large currents and capacitive loads
99. fusion Pressure Upgrade 4x 50 ml screw cap reservoirs 395 09 08n Perfusion Pressure Upgrade 8x 50 ml screw cap reservoirs 495 09 16n Perfusion Pressure Upgrade 16x 50 ml screw cap reservoirs 750 06 50 Replacement 50ml screw cap reservoir 12 with 50ml plastic screw cap reservoirs filter regulator and manifolds 09 04v Volatiles Pressure Upgrade 4 channel 732 09 08v Volatiles Pressure Upgrade 8 channel 1 090 09 16v Volatiles Pressure Upgrade 16 channel 1 942 with 35 ml glass syringes PTFE tube filter regulator and manifolds 06 30g Glass 30cc Syringes set of four 259 09 14 Oxygen8 Bubbled Pressure 4 channel 995 09 18 Oxygen8 Bubbled Pressure 8 channel 1 199 with 35 or 60 ml plastic syringes filter regulator and manifolds U S Canada prices shown International prices add 15 Email or visit web store for latest prices 29 www autom8 com Liquid switching times ee in milliseconds without moving sewer pipes A to 16 micro bore tubes into 1 outflow 360 Micron Removable Tip Flow rates 0 05 Gravity 1 15 ml min 2 psi 3 98 ml min 8 psi 10 66 ml min 250 Micron Removable Tip SF Flow rates 0 05 Gravity 35 ml min 2 psi 1 66 ml min 8 psi 5 16 ml min Perfusion Pencil Multi Barrel Manifold Tip a ae 100 Micron Removable Ti e Rapid solution change with micro liter dead volume P No piezo or stepper motor translation No clumsy rotating valves beer Fl
100. gned with electrode groups located at CA1 CA3 and DG 110 Effortless electrophysiology MED64 Probes Acute or cultured biological preparations are placed or grown directly on a grid of 64 planar microelectrodes with the capability for stimulation and signal recording The standard MED probe has 64 planar microelectrodes arranged in an 8 x 8 grid embedded in the center of a transparent glass plate The surrounding glass or plastic cylinder makes the MED probe a self contained recording chamber Four models with inter electrode spacings of 100 150 300 and 450 um enable detailed evaluation of network interactions across a sample Each electrode is 50um x 50pm in size for 150 300 and 450 um spacing configuration or 2Oum x 2Oum for 100 um spacing configuration Also available are probes featuring an electrode pattern specific to hippocampus anatomy and a hexagonal pattern as well as 2 and 4 well MED probes for increasing throughput MED64 Ordering Information Many sizes of probes electrodes and spacing available Please see web site for all sizes and part numbers Minimum order quantity 10 pieces on probes Please call or email for a quote www autom8 com Highly sophisticated user friendly software E F E E H mesg H E hma p T a Ja Pid LY iE mi Pe ah IH Ti it a CELTE ETTER Prefa eA PoE GE o aa 8 int Wy r F fa i F r a t a 4 P 3 i id E im a H
101. gy The ValveLink8 2 is less expensive than AutoMate Scientific s ValveBank controller It is the controller of choice for dose response work at pharmaceutical companies and the NIH Both ValveLink8 2s and ValveBanks are designed for use with pClamp Pulse et al All AutoMate Scientific products include a one year warranty ValveGuard technology detects bad valves to prevent damage to your ValveLink8 2 and easily observe problem valves Front panel LEDs are dark for broken or disconnected valves or blink for short circuited valves Automatic networking lets you connect up to eight ValveLink8 2s to a USB hub and PC to create a single 64 channel controller A ValveLink8 2 can power individual valves up to 1 amp 12 watts and a total of 2 amps for all valves open simultaneously ValveBank or ValveLink8 2 Which controller is right for you Features ValveBank ValveLink8 2 CHANNELS A or 8 channels available 8 channels each USB network to 64 channels COMPUTER I O 8 digital in 8 digital out serial RS 232 8 digital in USB analog input event marker out DIGITAL INPUTS One pulse can start a ValveBank program One TTL input per valve or demultiplex and or TTL inputs each control 1 valve control up to 16 valves with 4 inputs PROGRAMMABLE Ves ValveBank keypad EasyCode software or Only using real time analog or digital outputs digital outputs from your data acquisition software from your computer data acquisition software Mac and PC
102. h BackStop back flow prevention one way check valves U Z gt 2 U 7 lt a F T m z5 TI YY Zz inside the reservoir block prevent liquid backflow from the tip e Easy ports for refilling reservoirs e Convenient manipulator mounting e Luer lock tips are interchangeable with our standard Perfusion Pencil Integrated Pressure Regulator SmartSquirt Micro Perfusion System Ordering Information Precision regulator gauge 5 micron filter and electric valves are built in U S Canada prices shown International prices add 15 Email or visit web store for latest prices Manipulator not included 800 998 MATE 07 04 x SmartSquirt 4 Micro Perfusion System 1 670 07 08 x SmartSquirt6 MEE OnEE System l 2 169 The 0 10 psi regulator and air filter SmartSquirt Valve Pressure Unit Reservoir Block amp Perfusion Pencil eauieacompiessed av orother gas Systems with Valve Controllers source bottle compressor or lab air 07 VL 04 x SmartSquirt4 ValveLink Micro Perfusion System 2 665 07 VL 08 x SmartSquirt8 ValveLink Micro Perfusion System 3 164 07 VB 04 x SmartSquirt4 ValveBank Micro Perfusion System 2 765 See page 23 for flow rates 07 VB 08 x SmartSquirt8 ValveBank Micro Perfusion System 3 864 x specify 100 250 or 360 um i d removable tip or ZDV for new Zero Dead Volume Pencil
103. hanging shelf Optional non isolated sliding shelf oriented front to rear Full perimeter enclosure required to mount cage Type Il Faraday Cages BenchTop Faraday Cage The Type II Faraday Cage offers improved access and simplified assembly e BenchTop cage includes stainless The window shade type retracting front panel is easier to operate than steel baseplate hinged doors and causes less disturbance when adjusted This front panel a Peren MAUNA CABE AoE NGI eee may be positioned anywhere between fully opened and closed and stays eae Weneuenl ase in position without a fastener The front door is shipped assembled and the entire unit may be assembled in a few minutes with a screwdriver provided This cage incorporates the same stainless steel frame and copper mesh material as previous versions It mounts to and requires TMC s full perimeter enclosures and mounts to our 63 500 Series tables TMC Air Table Ordering Information TM 81 333 03 Type Il Faraday Cage 40 in 1 610 TM 81 334 03 BenchTop Faraday Cage 1 815 Complete TMC product line available Call or email for part numbers U S Canada prices shown International prices add 15 Email or visit web store for latest prices 800 998 MATE 97 an gt 2 gt CJ gt lt M gt GC m Y D N N ag TE N l N TE m ag lt L LI Z U lt L Isolator natural frequency Low Input V
104. he three above use 22 mm square bases UU Microscope brand and model 395 and accept 13 mm round 1 coverslips on top O Inverted upright 219 35 mm Petri dish holders N i e Zeiss Axiovert inverted 239 35 mm DISH 200 35N 35 mm Nunc Spacer rings each 20 FN1 Stage fixed adjustable 259 HOLDER 35W 35 mm WillCo 20 mechanical or third party like UNHEATED 35F 35 mm Falcon In Vitro D35 10 1 N No ring required 0 Narishige or Sutter 35C 35 mm Corning Mattek 20 Or ask us for a complete list 35V 35 mm In Vitro D29 10 1 N 20 of compatible microscopes U S Canada prices shown International prices add 15 Email or visit web store for latest prices Example part numbers QS H 12IN TT ZM QuickStage Heated 12mm diamond chamber Tool tray Zeiss M stage adapter QS U 35W C UU QuickStage Unheated 35mm Willco dish holder Clamp Universal stage adapter QuickStage Ordering Information sample combinations shown Heated QS H 12IN TT QuickStage Heated diamond 12mm perfusion chamber for ZM Zeiss or Leica M inverted microscope with Tool Tray gasket and 1 842 ThermoClamp 1 temperature controller QS H 35W C QuickStage Heated 35mm WillCo perfusion chamber with Pe ees perience ee UU clamp Universal stage adapter and ThermoClamp 1 2 057 temperature controller Two channel controller available 1 777 3 add l chambers many 35mm dishes amp stage adapters
105. hed Stainless Steel Sides and Base Textured Powder Coat Carbon Steel Top Plate Dimensions 18 w x 20 d x 3 h 458 x 508 x 76mm Weight 50 Ibs 22kg 100 Lightweight system with exceptional low frequency passive vibration isolation 66 SERIES Active TableTop CSP High Performance Vibration Isolation System with Compact Sub Hertz Pendulum Technology Benefits e Self leveling design with height control valves e Electropolished stainless steel chassis with powder finish steel top plate e Incorporates patented CSP isolator design e Lightweight low profile Features e Exceptional passive vibration isolation comparable to our full size industry standard 63 500 Series Tables e Lightweight Compact Design less than 50 lbs is easily portable TMC Air Table Ordering Information TM 66 501 TableTop CSP Isolation Systems 2 625 Complete TMC product line available Call or email for part numbers U S Canada prices shown International prices add 15 Email or visit web store for latest prices www autom8 com Heat your cells and Sababe media not the stage imaging TTL interface and footswitch mode activation Cold start acceleration Temperature output port for recording Heat shock activation Heated Lid power supply Remote setpoint port Temperature output for analog recording Bioptechs Delta T Controlled Culture Dish System Finally a culture dish system
106. ided to assist in selecting a stage adapter appropriate for your microscope Delta T Ordering Information Delta T Starter Kit Heated Dish Stage Incl Delta T Controller peels 10pk of Delta T Dishes Coverglass Lid Heated Lid with CO port 4 238 Culture Cylinder Pack and Standard Stage Adapter Bi 0420 4 03 Delta T4 Culture Dish Controller 3 163 Bi 04202003 Delta T Culture Dish Stage Adapters 96 Well Plate Sized 696 Bi 0420042105C Delta T Culture Dishes 10 pk 0 5mm thick glass clear 66 13 Bi 0420040500 ae a ee temperature control Culture Dishes 40 Bi 0420201918 Delta T Tissue Slice Adapter 443 Bi 0420201919 Delta T Brain Slice Adapter 506 Bi 0420081601 Delta T Hinged Perfusion Adapter Set 2 set 380 Bi 04200318 Delta T Cooling Ring 190 Bi 0420080316 Delta T Perfusable Heated Lid 474 pianoan ie Clr Conder tS s Bi 070303 01 Glass Culture Cylinders several sizes available 31 63 Bi 260700 Boekel Warmer 394 Complete Bioptechs product line available U S Canada prices shown International prices add 15 Email or visit web store for latest prices 800 998 MATE Stage Adapters 105 Micro Perfusion Pump Features Compact Usable as either single or dual channel Precise speed setting feature Flow rates of 0 2 to 180 ml hr Compatible with 1 16 tubing External DC power supply with internal 9 volt backup battery Ca
107. ighpass filter with frequency range 01 Hz to 100 Hz Output offset control to shift output up to 10 Volts Auto Zero feature to reset the output to the baseline level Notch filter to suppress line noise Digital Voltmeter on channels 1 and 2 Powerful output which can directly drive transducers Wide bandwidth greater than 500 kHz on all gains www autom8 com Precise The Brownlee Precision Model 440 is a rugged flexible and precise laboratory amplifier It was designed with particular attention to the needs of electrophysiology research These requirements include high gain low noise clean pulse response adjustable filtering removal of offset levels high input impedance and ease of use The 8 pole Bessel lowpass filter is ideal for removing high frequency noise while accurately amplifying the shape of input pulses Overshoot and ringing are negligible even with fast rise and fall times and at high gains There are 108 different cutoff frequencies available over a range from 20 Hz to 50 kHz The input bias current is also very low at 20 pA The input resistance may be set for 1 M or 10 Q These characteristics make the amplifier almost invisible to the source allowing the direct connection of many types of electrodes Easy To Use The Model 440 s front panel interface preserves the simplicity and feel of older analog instruments while taking advantage of the benefits of digital circuitry Changing an amplifier setting is
108. ilable from AutoMate Scientific Power provided by standard batteries Note that current is only drawn during pulse delivery DS4 Bi phasic Stimulator Voltage input ranges of 1V 2 5V t5V and 10V Output in 4 overlapping ranges of 10uA 100pA 1mA amp 10mA 48 V compliance provided GATE input allows multiple DS4 s connected to one DAC out 2a Additional Specifications DS2A amp DS3 Trigger A positive pulse between 3 V and 20 V is required to trigger these stimulators The trigger input current varies from 6 mA to 62 mA over the above voltage range Trigger pulse duration should not normally be less than 4 us DS2A amp DS3 Pulse Duration Range 20 us to 2 sec One dial allows continuous adjustment from 2 to 20 while another is used to select the range from 10 us 100 us 1 ms 10 ms 100 ms or external source with 10 accuracy Trigger Isolation Optical coupling is employed between the trigger source and the stimulator circuitry The capacity coupling is less than 3 pF Batteries DS2a amp DS3 11 x PP3 9V IEC 6R61 style batteries Current is only drawn when delivering a pulse Note that battery test sockets are built in Batteries DS4 The DS4 includes a 15V external power supply or accepts 10x 12V GP23A batteries Mounting One or two stimulators may be mounted in a 19 rack using a specially fabricated frame model D121 11 available from AutoMate Scientific Dime
109. ilter polycarbonate measured 0 78 0 79 pA The base material of the Stable tip has a thermal expansion coefficient of 23 um m C versus 70um m C of a polycarbonate holder That is an improvement by a factor of three Siskiyou Accessories Ordering Information SD Shuttle Three axis Chamber Shuttle System 3 100 SD PC H Horizontal Perfusion Chamber w 4 magnetic clips 130 SD PC V Vertical Perfusion Chamber w 4 magnetic clips 130 SD PC R Round Perfusion Chamber w 4 magnetic clips 130 SD 8090c Perfusion Chamber Platform 8 0 x 9 0 w drain 285 SD PC A Perfusion chamber adapter 10 8cm or 11cm diameter 290 Co 48510 00 Coverslips borosilicate 22x22mm square 1 156 pcs 54 SD ST50 200 HS Electrode holder fits Axon 200 700 amp HS 2A amplifiers 105 SD ST50 BNC Electrode holder fits amplifiers with BNC connectors 105 SD ST50 CV Electrode holder fits Axon CV 4 amplifiers 105 U S Canada prices shown International prices add 20 Email or visit web store for latest prices 800 998 MATE SAIYOSSIIIV NOAIMSIS Low profile variation Performance Specifications PZT head Travel 300 um Minimum controllable motion 0 3 nm Command input 10 V to 10 V MX1640 manual manipulator 76 Maximum load 2 lbs Travel axis 0 80 inch 20 mm Minimum controllable motion 5 um High speed piezo solution switcher Fast piezo solution switcher The piezo sol
110. ime TTL inputs to control valves Most acquisition software already being used in your experiments can talk to our controllers AutoMate Scientific offers an optional program called EasyCode for the Macintosh and PC Windows to program ValveBanks not ValveLinks This software is used before an experiment valve sequences are downloaded into the memory of the ValveBank where they are run An article by AutoMate Scientific can be found in Axon Instrument s AxoBits 17 newsletter outlining these strategies accessible on our web site 800 998 MATE Pinch Valves PTFE Inert Valves Lee Valves Luer lock Fittings in PTFE Inert Valves SARA E SSS i O x esa i a Fj r i pe Gi ae tae 2 E a F Li E ae a Luer lock fittings in PTFE Inert valves allow direct connection of syringe reservoirs for minimal dead volume PTFE Inert Valve Fitting Choices Hose Barb e Standard e Available for 1 8 and 1 16 i d soft tubing Lure Lock e For direct attachment of syringes e Eliminates extra tubing between reservoirs and valves e Includes 2 way stopcocks and 35 ml syringes Nut amp Ferrule e HPLC like screw in fittings for rigid small diameter 1 16 o d tubing 12 Perfusion Systems Ordering Information 17 21 20 ValveLink 4 2 Economy Pinch Valve Perfusion System 1 395 13 01 23 ValveBank 4 PTFE Inert Perfusion Syst
111. in order to apply thermal stimuli without introducing noise or interference It is also very suitable for intracellular ion imaging in cultured cells or tissue slices oocyte recording intracellular microelectrode recording or extracellular e g brain slice recording A Effect of flow rate on the static temperature and on the J rate of temperature change pe 40 4 7 A Static temperature at the Peltier element circles in TeC the solution leaving the Peltier element squares and at m g q J the cell triangles at flow rates between 0 6 and 1 7 ml m B 2 5 o fF min Command temperatures were 50 C and O C and os iO 15 feedback was from the Peltier element Flew mirni B B Temperature at the cell during step temperature 40 4 I F initia a commands at flow rates of 0 6 1 0 and 1 7 ml min The 1 L reo 20 4 W 4 0 mimin ie initial temperature at the heating cooling chamber was i 1 ae 32 C and the command temperature was stepped to O C for 30 s then back to 32 C D Reid et al J Neuroscience Methods 2001 Cool Solutions Ordering Information Heat Cooled Temperature Control System CO CS 1 CS 1 controller peltier cables two batteries special 0 1 C thermo 4 116 couple battery charger etc U S Canada prices shown International prices add 5 Email or visit web store for latest prices Manipulator not included 800 998 MATE 12V batteries and charger included f
112. ingle electrode voltage clamp for small cells with large currents e HVIC high voltage current clamp for extracellular applications such as iontophoresis www autom8 amp com Software Control Instead of the usual front panel knobs and switches the Axoclamp 900A amplifier is controlled by the Axoclamp 900A Commander a program that runs on a Windows PC computer and communicates with the amplifier via a USB interface This control interface reports resistance voltage and current measurements and provides automation of Bridge Balance Pipette Offset and Pipette Capacitance Neutralization In addition it has smart features that protect cells from potentially damaging signal oscillations and automate mode changes based on internal signal thresholds or externally applied signals Amplifier settings such as gain filter frequency recording mode and input output scale factors are automatically telegraphed to the pCLAMP 10 data acquisition software via the USB connection Optional SoftPanel Although the 9QOOA amplifier is a computer controlled amplifier the mouse and or keyboard is not the only means of controlling the instrument The optional SoftPanel was designed for those who prefer the more conventional feel to amplifier control of knobs and buttons By way of a USB connection the SoftPanel controller physically replicates all essential amplifier functions by acting as a hardware extension of the Axoclamp 9OOA Commander software SoftPa
113. ions with gas Each gas line can be individually adjusted Replacement stones also available Tygon PIFE Inert Silicone pinch valve and Polyethylene tubing available in any length over 10 feet 3 meters _ Vacuum pump and trap For labs without an easy vacuum source we offer a quiet Lab duty oil free vacuum pump with muffler for both 110V and 220V mains Our large 10 liter polypropylene Vacuum Trap means you won t have to empty it as often This Nalgene bottle includes easy luer lock fittings for vacuum tube connections and all necessary tubing to the pump and chamber www autom8 com Perfusion Accessories and Tubing Ordering Information 06 05c Case of 50x 5 ml syringes 35 06 15c Case of 80x 15 ml syringes 45 06 30c Case of 30x 35 ml syringes 55 06 60c Case of 20x 60 ml syringes 32 06 140c Case of 20x 140 ml syringes 180 06 1Kp essuie 1 Liter reservoir with bracket fittings amp tube 63 requires pressure 06 2Kp Pressurized 2 Liter reservoir with bracket fittings amp tube 84 requires pressure 06 10Kp essuie 10 Liter reservoir with fittings amp tube n2 requires pressure 05 04 Micro manifold 4 into 1 PTFE Inert with flow control 63 05 08 Micro manifold 8 into 1 PTFE Inert with flow control 84 05 16 Micro manifold 16 into 1 PTFE Inert with flow control 112 05 05
114. is over 90 The system possesses the same high quality noise free low drift and vibration free data acquisition as the PatchMAX 100A system Best value This system is more cost effective than a traditional electrophysiology setup In order to reach the same functionality and productivity using a traditional setup the equipment cost is more expensive plus labor cost Lower cost and better quality If you work on 50 oocytes per week using a traditional two electrode voltage clamp system now you can extract data from 100 oocytes per week www autom8 com ez SSEAL Pressure Controller MOO e2 gSEAL 1008 eis Peers Chapt Biriz impel The ez gSEAL 100B pressure controller is designed mainly for automated patch clamping but it can also be used for many other applications With the ez gSEAL patch clamping becomes as easy as a click of a button You can use it with our automated clamp systems for fully automated experiments or use it as a stand alone product on a traditional rig You can also use the controller for puffing drugs or studying stretch channels The software controlled pressure controller comes with pumps so air tanks are not required Key Features The ez gSEAL pressure controller makes patch clamping easy Click the first button to set the positive pressure the second button to set negative pressure for making seals and the third button to set the holding pressure for long lasting seals By clicking a single button
115. itimer Bi phasic Stimulus Isolator OUTPUT 210p 1 TIUA eal maA O OFF ona4 F 4 d maler bo mara mm ne Isolated Stimulators Ordering Information DS2A Constant voltage stimulator 1 199 DS3 Constant current stimulator 1 378 DS4 Bi phasic stimulator 2 420 D121 11 19 rack frame for two stimulators or DG2A 107 U S Canada prices shown International prices add 20 Email or visit web store for latest prices www autom8 com Compact free standing battery powered pulse generator DG2A Train Delay Generator The DG2A is a compact free standing battery powered instrument which can be used to generate trigger pulses required for repetitive stimulation Also featuring DELAY controls it is useful for determining nerve or axonal Effective Refractory Period ERP through the production of a delayed second pulse Various modes allow output pulses to be produced singularly SINGLE continuously FREE RUN amp GATED or in a burst TRAIN with the burst train duration and pulse frequency determined by the front panel controls In each of the modes except FREE RUN outputs can be initiated either by the front panel push button a TTL compatible trigger gating pulse or a suitable foot switch The unit has control of train duration over three full decades pulse repetition rate or frequency within that train over five decades and control of the delayed pulse over thr
116. kStage heated 12mm diamond chamber and stage adapter vac Vacuum Pump canister Vacuum Trap 17 11 57 ValveLink8 Lee Valve Perfusion System 01 05 x 2 Low noise Grounding Packages 01 20 Full BNC cable to DigiData 09 118 Oxygen8 Bubbled Pressure Kit Top Half 10 8 60 G BubbleStop Syringe Heater Bottom 03 18 250 ThermoClamp Inline Heater with 250pm tip S BSC1 Brain Slice Chamber 1 Interface and Submerged S PTCO3 Chamber Temperature Controller Custom BNC cables canister Vacuum Trap We offer a great web form to help build you own perfusion system Just answer a few questions enter your contact info and click Submit for a customized quote at http www autom8 com build_your_own html 800 998 MATE 9 am writing to tell you uie Increase reproducibility ValveBank8 Perfusion System e e i s great that can loa with fewer hours in lab the reservoirs press go and start recording can read papers rather than fussing with solutions and switching ner valves every 10 15 minutes F om Since solutions are switched pi 73 at exactly the same time ever i EST i ax from one experiment to the ol next have been able to automate my data analysis protocols as well a Brain Slice Field Recording 10 POPULATION SPIKE AMPLITUDE O PULSE 1 4 PULSE 2 50 uM THIOPENTAL el 2 M BICUCULLINE TIME minutes Una
117. latform or air table Footprint 0 75 19 mm adjustable in all axes extends to over 4 5 12 cm long Can be used to hold electrodes tubes manifolds temperature sensors glass capilliaries agar bridges etc Thumbscrews allow easy adjustment without needing any tools All stainless steel and anodized aluminum parts so they wont oxidize or corrode Start with our StageHands Kit 1 SH 1 which includes two magnetic bases with a pair of 1 5 4 cm arms one locking ball joint both a single and double tube electrode holder plus a separate manifold holder Add more arms holders or bases as desired www autom8 amp com N StageHands Ordering Information T R PartNo Product Description Price gt SH 1 StageHands Magnetic tool holder Kit 1 386 SH BJ TH StageHands ball joint arm with tube holder 216 Va SH ST DTH StageHands straight 2x arm with double tube holder 128 SH MH MS Manifold Holder Magnetic Screw Stand 65 O SH TH Tubing Holder 29 SH DTH Double Tool Holder 32 SH OFF RAB Offset Right Angle Bracket 15 SH RAB Right Angle Bracket assembly 18 SH STA Straight Arm 1 5 piece 16 aa SH RAA Right Angle Arm 1 5 piece 16 LY SH ISTA Interlocking Straight Arm 1 5 piece 23 SH IRAA Interlocking Right Angle Arm 1 5 piece 23 U S Canada prices shown International prices add 15 Email or visit web store for latest prices 800 998 MATE
118. lator 4 axis base mount with dovetail 4 450 Siskiyou Controller and Accessory Ordering Information SD MC1000e Std Controller 4 axis closed loop push button 1 025 SD MC1000e R T Controller 4 axis closed loop dial 3 025 SD MC1000e J Controller 3 axis closed loop Joystick 1 325 SD MC1100e Controller 4 axis target 1 550 SD SB MC1000e R eine cables for MC1000e MC1000e R and 75 SD JB MC1000e Std Junction box for MC1000e 615 SD JB MC1000e RO Junction box for MC1000e R 950 SD SB MC1100e Switch box and cables for MC1100e 75 SD JB MC1100e Junction box for MC1100e 930 SD SB MC1000e J Switch box and cables for MC1000e J 75 SD JB MC1000e J Junction box for MC1000e J 740 Siskiyou Hydraulic Manipulator Ordering Information SD MX630R Hydraulic manipulator 3 axis right hand 4 050 SD MX6600L Hydraulic manipulator base mount with round clamp 5 990 SD MX6600R Hydraulic manipulator base mount with round clamp 5 990 SD MX6600 45DL Hydraulic manipulator base mount with dovetail clamp 6 065 SD MX6600 45DR Hydraulic manipulator base mount with dovetail clamp 6 065 Siskiyou Headstage amp Pipette Holder Ordering Information SD MXC 45DR a a a mount for Axon 200 700 series and 220 SD MXC 45DL aaa a de mount for Axon 200 700 series and 220 SD MXC 45R Repeatable probe clamp rh 185 SD MXC 45L Repeatable probe clamp
119. lh 185 SD 2A 45D Axon 2A amplifier mount for MXC 45D series 35 SD CV4 45D Axon CV4 amplifier mount for MXC 45D series 35 U S Canada prices shown International prices add 20 Email or visit web store for latest prices 800 998 MATE 73 A TA A lt am Z4 gt gt AD WY Outfit your entire rig with Siskiyou accessories 3 Axis Shuttle System Shown in retracted position Horizontal E One inch working travel in XYZ Choice of 20 40 80 and 100 TPI K e gt adjustment screws Magnetic coupling pat pend allows quick with lt ee drawal of perfusion chamber from working area fusion Chambers Horizontal Vertical Round Vertical The PC series perfusion chambers have embedded magnets that are g conveniently located to retain perfusion plumbing and ground plugs T They are designed to fit into our 8090c fixed stage platform A standard Se E 22 mm cover slip is used to create the chamber bottom that is attached 4 with vacuum grease silicon or parafilm There are two plastic retention n screws for added security Both openings accept 13 mm round cover Round slips for cultured specimens PC series chambers come in three styles a horizontal vertical and round All models have two small but very Q gt powerful magnets located next to the input output reservoirs and two o more located closer to the recording chamber iis 74 www autom8 com
120. ll allow coarse positioning 800 um second www autom8 com axis Closed Loop Joystick Controller The MC1000e J joystick controller is designed for coarse micron level positioning of our 800 and 7000 series actuators manipulators and stages The joystick control is proportional from slow to high through the travel range of the joystick motion A two position rocker switch is conveniently located on the top of the controller The rapid setting is set to maximize speed 1 7 mm second when the joystick is moved to its farthest position from center The slow setting is set to maximize resolution 0 2 um 30 um second Polarity switches on the junction box allow the joystick motion to be set to match the output of the device being driven This feature ensures intuitive interaction between the joystick operator and the operation axis Closed Loop Push Button with Target The MC1100e uses the same control features as our popular MC1000e controller with a TARGET RETRACT feature on one axis The TARGET RETRACT allows the user to set a target location at a desired point When it becomes necessary to back away from the experiment area the user simply depresses the RETRACT button The stage actuator plugged into that axis then automatically retracts to its full negative limit The user then can return to the previously set position by simply depressing the TARGET button The MC1100e 4 axis controller has two preset sp
121. ll x 15 5 deep 6 5 kg 14 lbs Three externally amplified temperature sensors including an optically isolated bath sensor for virtually noise free operation 34 Your thermol RP channels wont know what hit them Cool Solutions CS 1 Peltier Thermal Stimulus Delivery System e Heat from 20 to 50 C in 3 seconds then cool to 2 C in just a few more seconds all controlled by your electrophysiology software e Display Peltier heatsink or bath temperature e Set the desired temperature from the front panel or from your computer The CS 1 can generate any desired timecourse of temperature change by simply converting a voltage from your interface into a temperature e Optically isolated bath sensor for low noise e Suitable for single channel no detectable noise added whole cell patch clamping or fluorescent ion imaging of groups of cells e Optional water cooling for low temperature work e wo output BNCs to monitor and record two temperatures externally e g Peltier and bath e All components included five minute setup e Tried and tested design used in major thermoTRP labs for over a decade see list of publications www autom8 com Peltier heating cooling element This temperature controller is for superfusing small areas e g groups of cells in a recording bath at a precisely controlled and rapidly adjustable local temperature The system has been especially developed for patch clamp experiments
122. loaded lock knob e Compatible with Axon 200 700 series HEKA EPC 10 and A M Systems model 2400 72 J raulic Integrated Base Mount Micromanipulators Smooth motion and instant response to hand adjustments makes the MX6600 ideal for long term intracellular recordings The water based hydraulic mechanism has a thermal expansion two to three times less than that of oil based systems thus minimizing drift to less than 2 um per hour at constant temperatures The MX6600 design integrates the metal bellow system with our precision crossed roller stage to ensure precision movement and long life The tine positioning dials on three axes use our ultrafine 1277PI adjustment screws to enable 0 5 um resolution The coarse positioning knobs on four axes incorporate 2OTPI screw drive positioning for smooth motion in the X Y Z and axes The axis can be adjusted to the desired angle of approach from O to 180 The MX6600 has our MXC 45 mounted to the axis The MXC 45 s built in rotational stop allows easy electrode pipette replacement MX6600s come standard with our ABP R mounting plate The design of this mounting plate enables coarse positioning between platform mounting holes as well as 360 of coarse rotational positioning If rotation with a solid submicron level stop is required the MX RS rotation stage is designed to mount directly into the ABP R as well as to the base of the MX6600 satable Headstage and Probe Cl
123. lutions Pressurized Local Drug Application Ordering Information 04 04 xxx Perfusion Pencil with 100 250 or 360pm tip 09 04 Perfusion Pressure Upgrade 09 18 Oxygen8 Bubbled 09 14 Oxygen8 Bubbled Pressure Upgrade Pressure Upgrade 04 08 xxx Perfusion Pencil with 100 250 or 360um tip 09 08 Perfusion Pressure Upgrade 09 18 Oxygen8 Bubbled Pressure Upgrade 800 998 MATE 7 ThermoClamp Heater 3 Temperature control AutoMate Scientific s standard perfusion systems are all room temperature by default but we offer several temperature control options 1 ThermoClamp inline heater 2 BubbleStop syringe reservoir heater 3 QuickStage stage heaters 4 Cool Solutions peltier heater cooler and 5 objective 03 18 xxx ThermoClamp Inline heaters Most of the thermal mass your cells experience originates from the Heat liquids flowing over them Therefore the most effective temperature control is a good inline solution heater or cooler which is positioned as close to BubbleStop the prep as possible Our ThermoClamp 1 maintains your solution and bath temperature to within 1 0 C often within 0 1 C using a thermocouple in your perfusion chamber and another in the heater It shares a similar design to our Perfusion Pencils mentioned previously allowing for rapid solution switching from a fine removable tip while also maintaining 37 C or any desired temperature between ambient
124. m SD MX110 Micromanipulator am pty i a 68 S D 10 Miniature Micromanipulator The SD 10 is a miniature micromanipulator ideal for positioning applications requiring micron level resolution DI100 series stages use a precision rolled 80 TPI lead screw for smooth positioning along their entire travel The miniature dovetail slide uses an adjustable spring loaded gib design This feature ensures stability straightness of travel and also works as a locking mechanism The device is equipped with a fourth axis which can be set at any angle through 360 of motion The compact size of the SD 10 makes it ideal for stimulator applications The mounting method makes removal and replacement very straightforward The addition of a simple stop allows repeatable placement SD MX110 Post mount Micromanipulator e Rotatable post mount e Mounting rod and base included e Maximum load 2 lbs e Travel axis 0 86 inch 22 mm e Resolution 10 um Unlike stages with rack and pinion drive the SD MX110 uses a spring loaded lead screw design against a solid stop which ensures drift free operation The SD MX110 incorporates fast pitch screw drive positioning and smooth ball bearing motion in the X Y Z and probe axes for precision positioning For added positioning versatility the top stage tilts at any angle from O to 90 and includes 22 mm of linear travel along the probe axis This feature plus 22 mm of X axis travel make the SD MX110
125. m The Coverglass Lid fits loosely on the Delta T Culture Dish and supports a 1 mm x 22 mm coverglass in the center of the field 3mm above the specimen The Coverglass Lid is reusable and helps the Delta T bridge the gap between an open dish and a closed 800 998 MATE 103 Heated Lid Cooling Ring Brain Slice Adapter 104 system environment The Coverglass Lid is made of 304 stainless steel autoclaveable and available with or without perfusion This item and or the Heating Culture Dish Cover are nearly essential for multi user facilities The Bioptechs Delta T Heated Lid is a device which will provide a condensate free optical surface on the top of a Delta T Dish through which specimens can be perfused and trans illuminated on an inverted microscope It is reusable and powered by a 2 5 volt source from either a battery or the optional auxiliary power supply in the Delta T Controller A CO2 port is included The Bioptechs Delta T Heated Lid w Perfusion is a device which will provide a condensate free optical surface on the top of a Delta T Dish through which specimens can be perfused and trans illuminated on an inverted microscope Specimens can be perfused by attaching perfusion tubing to the ports provided It is recommended to use the Bioptechs Micro Perfusion Pump with the dual perfusion tubing for this purpose The Bioptechs Cooling Ring is an immersion device which absorbs heat from
126. manual valves 35 ml or 60 ml syringes stopcocks reservoir bracket and drippers in PTFE Inert systems only ringstand 1 16 i d Tygon tubing and 4 8 or 16 into 1 micro manifold with flow control 5 15 35 60 or 140 ml syringe reservoirs available Visit www autom8 com build_your_own html to configure a perfusion system and quote Valves amp Fittings Ordering Information Each pair of 4 valves ordered together will be mounted in a case of 8 02 01 02 Set of 4 PTFE Inert valves cabled and mounted 895 02 pp 04 Set of 4 Pinch valves cabled and mounted 1 32 i d silicone tube 545 02 21 07 Set of 4 Lee mini valves cabled and mounted 1 095 02 01 02i Individual PTFE Inert replacement valve 125 02 pp 04i Individual Pinch replacement valve 90 02 21 08i Individual Lee mini replacement valve 6165 Low noise valve and case grounding package per 4 valves 01 05 A grounding wire attached to all valves extending back to the controller This 55 item is recommended for electrophysiology and required for CE conformity 02 06 Valve extension cables 2 meter RCA M F set of 4 cables 15 05 01 Luer lock fittings with 2 way stopcocks set of 4 10 05 02 Nut amp ferrule fittings for 1 6 o d tubing set of 4 30 U S Canada prices shown International prices add 15 Email or visit web store for latest prices 02 TB 02 Indicate Tlop inflow and B ottom outflow PTFE Inert valve fittings
127. mm dish Separate perfusion inflow aspiration outflow gas connection temperature sensor and grounding provided 20 www autom8 com QuickStage Part Numbers QS Heated Perfusion Chamber Retainer Microscope Stage Adapter CELL CHAMBER 1450 12IN 12 mm diamond round coverslip inverted 85 TT Tooltray 48 ZM Zeiss Leica M 259 HEATING INSERT 5IN 5 mm diamond round coverslip inverted 85 C Clip 23 ZK Zeiss Leica K ASI Prior Marz 239 with ThermoClamp 12UP_ 12 mm diamond round coverslip upright 85 Sutter MT MP 78 controller LS large slice chamber upright 85 ZP Zeiss Leica plane 239 The four above use 24x40 mm rectangular O Olympus Narishige Gibraltar 219 1 coverslips bases one included Scientifica ThorLabs 110mm ring 35 mm Petri dish heaters TT Tool tray 48 N Nikon Siskiyou ring 108mm ring 239 35 mm DISH 1590 35N 35 mm Nunc Spacer rings each 20 C Clamp 52 FN1 Nikon FN1 259 HEATING INSERT 35W 35 mm WillCo 20 UU Universal adapter 395 with ThermoClamp 35F 35 mm Falcon In Vitro D35 10 1 N No ring required 0 controller 35C 35 mm Corning Mattek 20 35V 35 mm In Vitro D29 10 1 N 20 QS U nheated Perfusion Chamber Retainer Microscope Stage Adapter CELL CHAMBERS 0 H horizontal diamond 130 TT Tooltray 48 ZM Same choices as above 259 UNHEATED V vertical diamond 130 C Clamp 52 ZK 239 R round 130 ZP Tell us your 239 T
128. mp Tubing Assembly 4 pk C Flex 137 50 Bi 16181303 Perfusion Pump Rod Mounting Clamp 38 Complete Bioptechs product line available U S Canada prices shown International prices add 15 Email or visit web store for latest prices 800 998 MATE This intuitive control interface system is optimized for time lapse imaging of live cell activity It provides a convenient accurate reliable and repeatable method of controlling fluid and temperature for chemically or thermally induced change experiments in live cell chambers It is WYSIWYG on both Mac and Windows platforms USB Data Acquisition Function Module Plugs into the USB port on Windows or Macintosh computers Pumps Plug the pumps into the USB Data Acquisition Function Module and control two separate perfusion sources Computer Control Control and Record Experiments Plug the FCS2 Delta T or Objective Heater into the USB Data Acquisition Function Module and have temperature control of your experiment with the ability to record temperature data 107 2 Lu Y gt Y cap O C Lu 2 lt 12 A lt L Applications Include Evoked or Spontaneous Activity fEPSPs Spiking Synaptic Plasticity LTP LTD Spontaneous Rhythmic Activities and Oscillations Pacing and Propagation in Myocytes Safety Pharmacology CNS Toxicity and QT Prolongation Chronic Drug Testing with Cultures Stem Cell Research Am
129. n Detachable ZK Zeiss K Non Detachable Electronic Axiovert 100 Marzhauser Scan IM 130X100 ZK Zeiss K Axiovert 135 Axiovert 200 Axio Observer Axioskop 2 FS Axioskop FS LSM META FS MOT Non Detachable UU Universal Mechanical Non Detachable UU Universal Axio Examiner LSM 710 NLO Various Options Please email IX50 Mechanical Non Detachable O Olympus Ring IX70 IX51 Detachable UU Universal X71 IX81 IX51 Electronic Marzhauser IM 120X80 ZK Zeiss K 1X71 IX81 BX FS WI Olympus Bridge Stage WI XYS Stage Fixed O Olympus Ring Microscope Mover Fixed Stage Narishige Microscope Translator ThorLabs Mechanical Non Detachable BX FS WI Mechanical Non Detachable UU Universal BX Stage Focus BX51 TF TRF BX61 TF TRF BX FS WI ThorLabs Avt Mounted Stand O Olympus Ring Electronic Stages if the insert cannot be definitively identified the customer should test that the stage adapter does not interfere with stage www autom8 com Microscope NIKON Orange rows not available LEICA 800 998 MATE Configuration Inverted Upright Inverted Inverted Model Stage Type Diaphot TMD TMS Diaphot 200 Diaphot 300 TE200 TE300 Ti Mechanical Ti Mechanical Detachable Single Plate or Non 3 Plate Non Detachable AutoMate Stage Adapter N Nikon Ring Detachable N A Ti S E Nikon Moto
130. n be operated on internal battery if desired Computer interface available 106 Program and record perfusion flow and temperature over USB a at a i _ 4 ig Perfusion Pump and Software When imaging cells in a rigid structure such as a chamber having a coverslip as an observation surface one must appreciate the fact that any rapid changes in flow rate will translate to microdynamic changes in pressure within the optical cavity of the chamber This causes the coverslip to behave like a diaphragm thus flexing out of focus due to the narrow depth of field of the microscope objective To eliminate this problem at the lower flow rates and significantly reduce it at higher flow rates Bioptechs recommends the Micro Perfusion pump for use with all its micro observation systems The Micro Perfusion Pump is a miniature single or dual channel full featured peristaltic pump designed specifically for low flow rates Unlike most peristaltic pumps that are driven by stepper motors the Micro Perfusion pump is driven by a tachometer regulated multi stage DC gear motor This assures a smooth analog rotation of the roller spindle free of instantaneous steps It is regulated by either the internal control circuitry adjustable trom 0 2 180 ml hr or it can be interfaced with a computer through a DAIO port Bioptechs provides a kit for this purpose that includes all cabling software and hardware needed for a dual pump setu
131. nd a variable LAG control output gain with 1000 fold dynamic range 4 pole Bessel filter onboard leak subtraction dual external command inputs versatile panel meter displaying Holding Command rms current noise membrane potential tracking potential and current at the headstage input telegraph output of values for output gain filter frequency headstage mode gain and measured cell capacitance ZAP and dual speed current clamp Also with the Axopatch 200B amplifier the bath is grounded for convenience of use and straightforward addition of command and compensation potentials 800 998 MATE Quiet Single Channel Recording Integrating Headstage Mode With unprecedented low noise and superb linearity the Axopatch 200B capacitor feedback integrating headstage is ideal for measuring sub picoamp current signals Bilayers Headstages useful in artificial bilayer experiments must be stable with large input capacitance loads The Axopatch 200B amplifier is rock solid with an input capacitance of 1000 pF Superb Whole Cell Performance Resistive Headstage Mode In whole cell recording more current noise is produced by the cell and the environment than by the patch clamp amplifier Hence the benefits of a low noise capacitor feedback headstage cannot be effectively utilized in whole cell mode For this reason the Axopatch 200B amplifier uses traditional resistor feedback headstage electronics for the whole cell mode of
132. ne preferred Battery Life Approximately 250 Hours with Ever Ready 6LF22 or Duracell MN1604 Dimensions Size 188 x 110 x 60 mm wh d Weight 490 grams 1 lb with battery fitted Please note that no accessories other than a battery are supplied 56 Specifications of the DG2A The unit always produces a pulse to synchronize other equipment at SYNC and a pulse delayed from SYNC by DELAY controls e Modes Control Four position rotary switch Movement of this control will immediately terminate any cycle and keep it in a Reset state for about 1 second This is a useful feature should an exceedingly long cycle be selected by accident Functions SINGLE Input signal triggers a single Output pulse FREE RUN Continuous Output pulses as set by REPETITION controls GATED Input signal enables unit to produce pulses as set by REPETITION controls TRAIN Input signal Triggers unit to produce pulses as set by REPETITION controls for the time as set by the DURATION controls e Input Connector BNC socket Levels TTL high gt 1 5 V TTL low lt 0 8 V Polarity Active High GATE TRAIN and Positive edge SINGLE or Active Low and Negative edge by Internal Jumper Internal Jumper Enable Active Low allows a Low Input during its active phase The front panel provides a check box to mark with a waterproof pen when enabled In Active Low an external contact closure can be used Push button Has same function as a valid
133. nel knobs replicate continuous mouse controls gliders while buttons replicate single click mouse controls 800 998 MATE Comprehensive Manual We also provide a detailed User Guide that serves as a handbook of procedures for microelectrode users Tutorials written by MDS Analytical Technologies staff and scientific consultants provide a useful guide to the operation of the instrument and are informative references for several electrophysiological techniques More Information The Axoclamp 900A main unit comes standard with e One Remote BUZZ Box works for both channels e One Clamp 1U model cell e Two HL U electrode holders e One Axoclamp 900A Commander software CD e Two USB 2 0 cables e Two headstage baseplates e Theory and Operation User Guide printed Two HS 9A headstages e g HS 9A x0 1 HS 9A x1 or HS 9A x10 must be ordered with the Axoclamp 9OOA 83 gt gt L Q M gt lt a ey lt L A Q gt lt lt L Advantages Pipette Offset The Axopatch 200B amplifier provides 250 mV of offset potential Seal Test The convenient seal test may be used in voltage clamp mode 5 mV pulse or in current clamp mode 50 pA B 1 or 500 pA B 0 1 Dual Command Potentials Two separate command potential inputs allow you to sum command input signals from two different sources The back panel command is scaled to afford greater range up to 1V and so is quite
134. ng plate Many manufactur ers of micromanipulators provide custom mounting brackets for Axon CNS headstages Clamp 1U model cell 87 gt gt L Q z M Z Y M Q lt U Q zZ m N LL oc lt L Li O Y a 2 lt L LJ A New Features Membrane Test calculates Ra and Cm per sweep during a recording Membrane and Seal Tests combined into a single resizable window Support for four stimulus waveforms with the Digidata 1440A digitizer Control of eight digital outputs per epoch during a sweep Control of split clock sampling per epoch during a sweep Leak subtraction automatically saves both raw and corrected traces All protocol durations are entered in time units Support of the new Digidata 1440A digitizer Support of the new Axoclamp 9OOA amplifier New ABF 2 0 file format Applications pCLAMP 10 is suitable for a wide variety of applications Uses e Action Potentials APs Current clamp Electromyography EMG Electrooculography EOG Excitatory Post Synaptic Currents EPSCs Excitatory Post Synaptic Potentials EPSPs Inhibitory Post Synaptic Currents IPSCs Inhibitory Post Synaptic Potentials IPSPs Long Term Depression LTD Long Term Potentiation LTP Miniature excitatory potentials Minis Ratio dyes with PMTs e Single channels e Slices e Spike trains e Synaptic networks e Voltage clamp e Whole cell
135. nism Screw down Material Aluminum Contact resistance lt 30 milliohms Printed Wiring 4 layers rejects hum noise by a multi shield structure Dimensions W174 x H21 x D150mm Weight 480 g X Le 7 Systems a J pesame rer ye MED probe U 50 um PtBk MCS 30 um TiN MED probe O 20 um PtBk 10 100 1 000 10 000 Frequency Hz 109 gt UO aie gt lt m CO On F N x lt N m _ lt Y Lu CO OC a ae aF O C Lu 2 lt E Q an lt L Specifications Substrate Glass Substrate 50 x 50 x 0 7 mm Glass cylindrical chamber ID 22 OD 25 mm Conducting layer Indium tin oxide ITO 0 15 um Insulation layer Polyacrylamide 1 4 um Recording stimulating electrode Number of electrodes 64 61 for MED 2Hxxx Material ITO Platinum Black Size 50 x 50 um square 20 x 20 um MED P210x Diameter 20 um MED P2HO7x 50 um MED P5001x amp P5002x Impedance lt 22 kQ MED P5xxxx lt 40 kQ MED P2xxxx 1kHz 50 mV sine wave Maximum voltage 1 V Maximum current 200 pA 0 1 msec Reference electrodes Number of electrodes 4 Material ITO Platinum Black Size 200 x 200 um Diameter 100 um x 4 MED P2HO7x MED P5001x Impedance lt 2 2 kQ 1 kHz 50mV sinusoidal wave Available in two chamber depths and four inter electrode spacings Hippocampal slice on our MED P5001A probe desi
136. nsions Panel size 190 mm x 110 mm Weight 800 g complete with batteries Output terminals A pair of 2 mm touch proof sockets on the front panel spaced at 0 75 Indicator An LED operates for the duration of each output pulse 54 Technical Specifications e Output DS2A Mk Il Constant voltage Two ranges provide 99 V high and 9 V low maximum output A multi turn dial allows output to be selected as a percentage of maximum Square wave pulse profile with typical rise time lt 1 us and fall time lt 3 us into resistive load DS3 Constant current Output between 2 pA and 32 mA Control is achieved by a variable range switch with four selections 10 pA 100 pA 1mA 10 mA and a three turn dial Pulses from high impedance stimulators constant current units can result in cells charging up between stimuli leading to stimulus loss This problem has been overcome in the DS3 which has an Output Discharge Clamp Circuit that operates for 20 us after each stimulus pulse This will discharge cells with capacitances as high as 1000 pF DS4 Bi phasic Bi phasic constant current proportional to the input voltage up to 5 kHz signals 10uA 100yA 1mA 10mA for a full scale input gt 2us duration An inactivity sensor reduces battery usage and damaging leak currents during infrequent stimulation while maintaining low levels of zero crossing distortion for repetitive waveforms DS4 Dig
137. o minimize bubbles Dual channel ThermoClamp 2 Temperature Controller For simultaneous heating of Perfusion Pencil inline heater and microscope stage 24 Accurate temperature control rapid solution switching and fast wash out ThermoClamp 1 Temperature Control System Combination inline heater plus multi channel focal drug delivery Maintain bath temperature and rapid drug wash out with a high flow bath line while quickly switching 4 8 preheated solutions through the Perfusion Pencil Steady 37 C at 5 ml minute flow rates through both the bath line and tip Advanced auto tuning temperature lock Fuzzy logic PID software maintains chamber or reagent temperature to within 1 C of setpoint or better The ThermoClamp calibrates its own tuning for ideal temperature control no need to guess loop speed settings Designed for physiology research No metal anywhere in the flow path unlike some competitors Low noise for electrophysiology with internal and external grounding plus electrical isolation between liquids and heating elements Ready to use Includes everything you need for heated perfusion power supply temperature sensors and inline heater with easy luer lock tube connections www autom8 amp com You can have rapid switching fast wash out and accurate temperature control at the same time Set the ThermoClamp temperature from ambient to 50 C A front panel BNC provides an analog output of
138. oLog case where further amplification of the signals can be selected on a channel by channel basis Further filtering can be carried out by the various NL134 5 6 or NL144 FILTERS while the signal can be conditioned for ADC input using the NL530 CONDITIONER 800 998 MATE ransducer Measurements The NL108A PRESSURE AMPLIFIER provides an easy method of monitoring physiological pressure changes and can be used in combination with your own or our disposable NL108T2 reusable NL108T4 shown opposite pressure transducers A pressure transducer and appropriate lead connects to the NL108A module allowing continuous monitoring of parameters such as blood or intra tracheal pressures The output of the NL108A can be fed directly to a chart recorder or ADC interface for PC based acquisition eneration amp Electrical Stimulation The NeuroLog range includes several modules capable of pulse generation Pulse patterns can be pre defined in a variety of ways allowing you to control other modules within the NeuroLog rack or send TTL compatible trigger pulses to external devices such as stimulators or acquisition systems The NeuroLog range includes the small constant current NL8OOA STIMULUS ISOLATOR which can be controlled by other NeuroLog modules Our new NL512 BIPHASIC BUFFER allows one or two NL8OOA s to be controlled by an analog waveform such as that produced by a computer controlled DAC NeuroLog Modular System
139. olution temperature changes As solutions warm they cannot hold as much gas so they off gas This forms bubbles in perfusion tubing It is especially true in inline heaters where the temperature rises dramatically The best cure for bubbles is to pre warm your solutions AutoMate Scientific makes the BubbleStop Syringe Heater with integrated temperature sensor and feedback Set the temperature on the BubbleStop a few degrees higher than room temperature or your inline heater and the warmed solutions will off gas in the syringe reservoirs to the maximum gas concentration for that temperature Even if they cool and re heat again inside the perfusion tubing they will not off gas BubbleStop Ordering Information 10 4 60 G BubbleStop4 60ml Syringe Heater Gravity 750 10 8 60 G BubbleStop8 60ml Syringe Heater Gravity 900 10 4 60 P BubbleStop4 60ml Syringe Heater Pressurized 1 100 10 8 60 P BubbleStop8 60ml Syringe Heater Pressurized 1 300 10 4 35 G BubbleStop4 35ml Syringe Heater Gravity 750 10 8 35 G BubbleStop8 35ml Syringe Heater Gravity 900 10 4 35 P BubbleStop4 35ml Syringe Heater Pressurized 1 100 10 8 35 P BubbleStop8 35ml Syringe Heater Pressurized 1 300 Including 6Oml or 35ml syringes stopcocks and tubing Pressurized version includes regulator filter and gauge U S Canada prices shown International prices add 15 Email or visit web store for latest prices ww
140. on tubes meet in a smaller manifold with a tiny tip ideal for squirting solution directly at a patch or region of interest AutoMate Scientifics Perfusion Pencil can achieve switching times 04 08 250 Perfusion Pencil below 10 milliseconds WITHOUT the need of steppers or piezo switchers This manifold combines 4 8 or 16 tubes into a single removable tip with a Perfusion Pressure Upgrade micro dead volume of 2 3 ul When the perfusion system s valves upstream switch from one liquid to the next the change happens inside the Perfusion Pencil tip resulting in very fast switching times For researchers who need faster switching times steadier flow rates fine microliter delivery or small pipette tips we recommend adding a Pressure Upgrade to the perfusion system These pressurized syringe reservoirs with regulator push liquids out much faster than gravity especially with small Perfusion Pencil tips Air pressure is much more quiet electrically and audibly more smooth and more reliable than peristaltic pumps 09 08 Perfusion Pressure U d Electrophysiologists often need their solutions bubbled with Carbogen for ciel slice oxygenation For many years AutoMate Scientific s Pressure Upgrade Kit was incompatible with bubbling because the syringe reservoirs are Oxygen8 Bubbled Pressure closed air tight However we have created the new Oxygen8 Bubbled Pressure Upgrade which simultaneously bubbles and pressurizes all of the so
141. ontroller can be used for pressure clamping for stretch channel study The pressure sensitivity is 1 5 mmHg and the pressure range is from 250 mmHg to 250 mmHg good enough for most stretch channels Pressure control for drug application The ez gSEAL pressure controller can also be used for local drug application A series of pressure pulses can be given at defined pressures for defined durations PatchXpress Testing The auto seal algorithm is similar to what is used in the PatchXpress automated patch clamp system PatchXpress users can use it to test their seal parameters under a microscope for easier trouble shooting and lower costs 119 M a6 gt A m z gt gt L QO m py GC Y m gt Live cell imaging like youve never seen if before N LLI j sf i gt Q O LJ O ad p 2 co Ask for 3 free samples Biomicroscopy Slides lide Luer High resolution microscopy of living and fixed cells e Combination of a cell culture chamber and a coverslip for imaging inside a channel e Immunofluorescence stainings with easy liquid exchange e Real time imaging during staining process e Luer connections available for easy flow applications in a channel e Channel volumes of 25 ul 50 ul 100 pl 150 ul 200 ul stigate chemotaxis and migration of adherent cells in linear gradients e Chemotaxis of cancer endothelial cells fibroblasts etc
142. or noise free heating and cooling Special 0 1 C error thermocouple for exceptional accuracy Transition from 2 C to 50 C BO 5 I 50 4 Ju o Temperature C D Thins ta 2 4 i SE E E 1 it Tima Sandi o Transition from 36 C to 2 C 60 5 50 4 P40 a p Trane bma 20 3448 10050 in Ai a i0 E a 10 20 Trma patercericis 35 just wanted to tell you Perfuse microliter volumes that have been using the SmartSquirt nearly every day k 9 and am very pleased with of precious solutions it have tested for cross contamination and believe it is very low To prevent hydrostatic flow from the cryostat tubes into the bath one line must continue perfusing bath solution after all other valves are closed zZ Y Li or Li O Ge 2 as CS Y ox lt L 2 Y Your design changes help hold all of the fittings tightly and keep them from leaking or flowing backwards would also rate your company as being excellent Cee amp EA Agog Ooo Gog COCO oc ry for your immediate response to problems and questions on your products Dr John G Starkus The Queen s Medical Center Honolulu Hawaii SmartSquirt Micro Perfusion System e Deliver microliter volumes starting with only 100 microliters of precious drugs Switch between eight solutions in milliseconds from a single tip All tubing now PT
143. ow rates e Single cell and patch delivery Gravity zero flow For mounting on any micromanipulator Easy to connect and clean 2 psi 0 07 ml min 8 psi 0 25 ml min e Vacuum cross contamination prevention Use one tube to vacuum the tip volume clear between solution changes 100 250 and 360 micron internal diameter removable delivery tips available Perfusion Pencil Ordering Information Multi barrel Perfusion Pencil 4 into 1 with 04 04 x 225 100 250 or 360 um i d tip 04 08 x Multi b Perf P P 8 1 with 225 Alt tubing 08 ti barrel Perfusion Pencil 8 into 1 with ti X uiti usi I INTO WI Ip now PTFE 04 16 x Multi barrel Perfusion Pencil 16 into 1 with tip 350 lined 04 x Replacement removable tips 30 x specify 100 250 or 360 um i d removable tip or ZDV for new Zero Dead Volume Pencil see ThermoClamp page U S Canada prices shown International prices add 15 Email or visit web store for latest prices 800 998 MATE 23 WINJd NOISNdJHdd gt 1 1 J21 5 i Heated Perfusion Pencil with Neoprene Sleeve Removed Easy luer lock connections and manipulator mounting Thermodynamic design maintains temperature with 5 ml minute flow from both tips without any metal tubing Separate chamber bath and Perfusion Pencil thermocouples included See previous page for flow rates See next section for syringe warmer t
144. p The pump comes with an external 9 volt AC adapter and also contains an internal 9 volt battery which can function as the primary power supply if needed www autom8 com The pump includes a single 062 D tube and a dual tube that has two 062 D tubes for use as a dual channel pump Although other tubing sizes are available this size is generally best suited for imaging applications The pump tubes are available in silicone rubber and C Flex They are terminated with a 1 16 tubing barb The base of the pump is threaded for easy mounting to a stand or fixture near the microscope Perfusion Temperature Control Computer Interface P iiuimn i ampare Cantales Filtre Fima lem Eigir Uber G24 Caniral Valaes al mie ee peor ipn Ba The live cell microscopy Perfusion and Temperature Control Interface from Bioptechs features e Extreme ease of use with precise and repeatable control e Dual or single micro perfusion pumps e Flow profile to reduce dead volume delays e Temperature and perfusion recording e Data logging of perfusion and temperature e Multi flow rate calibration e Temperature profiling and cycling e Saving and reloading settings e Graphic display of events Perfusion Pump Ordering Information Bi 60319131616 Micro Perfusion Pump specify desired flow rate s 1 195 Bi 13161603 13 Perfusion Temperature Controller USB interface 3 680 Bi 60319192016 Single Channel Pu
145. pA Offset Voltage 15 uV max Voltage Range 10 V CMRR gt 70 dB all gains Noise 1 5 pV 10 pV rms 10 kHz peak to pea Output Voltage Range 10 V Current Capability 100 mA Slew Rate 10 V uS Output Offset Control Range 10 V in 100 mV steps Noise 500 uVins 3 mV ea to sat 10 kHz Auto Zero Pressing Auto Zero will reset the output to the baseline level when the Highpass Filter is on and self calibrate the amplifier s internal offset voltages DVM Channels 1 and 2 include a handy Digital Voltmeter to quickly and easily measure the input level 47 A complete solution to your electrophysiology needs NL905 Small Case System NeuroLog Modular System and Power Unit l Ao The NeuroLog System provides a modular and highly versatile means of carrying out intracellular extracellular or transducer based recordings signal conditioning pulse generation or electrical stimulation within one compact device The NL9OOD Case amp Power Supply unit allows up to thirteen modules to be installed This means a single NeuroLog System can be used to amplify This smaller case holds four several different parameters such as extracellular spikes intracellular NeuroLog modules instead potentials or even blood pressure as well as produce outgoing trigger pulses of thirteen to other pieces of equipment or electrically stimulate a preparation ification amp Signal Conditioning
146. pensate for the elimination of the front tiebar in order to provide kneewell space Rugged Built in Leveling Feet Table legs include built in fine thread 3 in 75 mm diameter screw jack levelers with 1 2 in 13 mm travel provision for external adjustment and a handy adjustment wrench The base is a solid slightly domed shape to assure solid wobble free contact with sloping or irregular floors Superior Table Tops Our standard laminated tops provide an attractive stainless steel ferromagnetic working surface with highly damped high stiffness construction at low cost For applications requiring the ultimate stiffness and damping or mounting holes specify TMC s patented CleanTop Il honeycomb top Accessories for 63 Series Tables may be fitted with armrests and rigidly supported sliding side shelves just above the table top The sliding shelves must be used in conjunction with a front support bar and a rear support bar that are fastened to the table legs The bars can be retrofitted but cannot be used with the full perimeter enclosure or Faraday Cage Front Support Bar This adjustable steel rail mounts on the table s front legs It has a slot in which the shelves mount and is normally ordered with the armrest pads The bar may be centered along the length of the table or cantilevered to suit your application www autom8 com Rear Support Bar This adjustable rail mounts on the rear table legs and supports the rear end of the
147. peting valve drivers open single or multiple valves master channel for control buffer solution computer control e Low noise amp low voltage valve control Designed for electrophysiology CE marked for Europe e Manual TTL digital analog and USB inputs Control valves manually by pushbutton or by computer simultaneously thanks to the microprocessor design Optional spill sensor protects your equipment when a leak is detected 800 998 MATE 13 ValveLink8 2 Controller e Manual pushbuttons e Red green LED indicators e 1 5 amp 12V AC supply included e Dimensions 9 28 x 1 6 x 5 13 e Weight 3 lbs 1 4 kg e Eight TTL inputs directly activate 8 valves e Or control 16 valves with only four digital outputs Additional Features e One analog input can control eight valves e Analog event marker allows you to record all valve activity An optional spill sensor stops all valves when a leak is detected to protect your microscope and table All LEDs blink until you press a button to continue Free ValveLink PC Software e Control valves directly from your PC screen by USB e Network multiple ValveLinks into a single virtual instrument 14 ValveLink8 2s can switch 12V DC solenoid valves open and closed in one millisecond using full power then hold in at 12 power to prevent thermal transfer to your solutions Low noise circuitry minimizes recording artifacts in electrophysiolo
148. plifier Specifications MED64 Head Amplifier e Number of Channels 64 e Input Impedance 100 MQ e Output Impedance 10 kQ e Gain x10 60 dB e Bandwidth 0 1 Hz to 100 kHz OdB to 3dB Built in Stimulator e Number of Channels 2 e Output Format Constant current e Maximum input voltage 4 V e Max output current 200 pA MED64 Main Amplifier e Gain 1 217 e Bandwidth 0 1 Hz to 10 kHz OdB to 3dB e Analog Low Cut Filter 0 1 1 10 100 Hz 12 dB oct Built in USB Digitizer e Resolution 16 bit e Sampling rate 20 kHz General e Power supply e AC 100 240V 50 60 Hz e Internal power 12V DC e Weight 6 6 5 9 kg e Dimensions W430 x H74 x D437 mm x two boxes 108 Watch the whole network without pulling electrodes MED64 Multi electrode Array Acquire continuous reliable long term evoked and spontaneous field potentials across 64 channels with two dimensional real time analysis of neuronal myocardial activity without the difficulties of a patch clamp rig Features Record extracellular signals across 64 channels without pulling glass electrodes e Evoked potentials or spontaneous activity fEPSPs spiking are acquired with 64 planar microelectrodes patterned on the patented MED probe e Any of the 64 electrodes can be used for stimulation up to two at once e Can be used with patch clamping imaging and external stimulation e User friendly software guides you through experimental s
149. r reliable valve controller ak ete Sie ae noid valves The ValveBank and accompanying EasyCode Macintosh software allow us to program full wash and delivery sequences in advance with significantly more accurate switching The new manual perfusion timing option allowed the ValveBank to perform exactly like the controller we had built before Basically the ValveBank saves us the worries of monitoring reagent delivery and it works Dr David Julius Department of Cellular and Molecular Pharmacology University of California San Francisco ValveBank Controller Made to last e Run experiments automatically even unattended A a By running experiments automatically AutoMate Scientific systems will leave you free to accomplish other tasks saving you both time and money e Microprocessor based for accuracy and flexibility The ValveBank can store sixteen user programs with 10 millisecond switching accuracy It includes powerful perfusion commands and capabilities not offered by competing valve drivers open single or multiple Original ValveBank from 1994 still valves master channel for control buffer solution and computer control in daily use in David Julius lab at UCSF Photo taken July 2013 e Low noise amp low voltage valve control Designed for electrophysiology CE marked for Europe e Manual TTL digital and serial RS 232 inputs Control valves manually by keypad or by computer simult
150. r mounted to an adjustable clamp on the axis This clamp allows the MXC 45 and axis to be adjusted to the desired angle of approach from O to 180 for true axial approach The MXC 45 s built in rotational stop allows easy pipette replacement The MX7600 can be used with our e series and MC2000 controllers to drive the 7600 stage through a closed loop interface between the controller and the 800 998 MATE Features e 1 7 mm second rapid positioning e Axial approach on 4th axis e Repeating probe holder Performance Specifications e Maximum load 2 lbs e Travel axis 0 80 inch 20 mm e Minimum controllable motion 0 1 um e Backlash lt 5 um e Point to point accuracy 2 um 69 motor encoder The closed loop connection ensures 0 2 um and 0 1 um resolution respectively MX7600 series stages come standard with our ABP R mounting plate The design of this mounting plate enables coarse positioning between platform mounting holes as well as 360 of coarse rotational positioning If rotation with a solid submicron level stop is required the MX RS rotation stage is designed to mount directly into the ABP R and has mounting holes to attach to the base of the MX7600 The MC1000e is a simple push button controller with submicron positioning capability that is compatible with our 800 and 7000 series actuators manipulators and stages The MC1000e 4 axis controller has two preset speed settings rapid
151. rds Accepted Prices are in U S Dollars plus any applicable sales tax and shipping charges Orders originating outside the U S or Canada will be charged higher prices than shown in this catalog generally 15 due to increased shipping regulatory and communication expenses Our web store will show accurate international prices for registered guests or you can always request a quotation Purchases may be charged to a Visa or Warranty MasterCard account or placed via a Purchase Order showing billing and shipping addresses as well as end users name address department and complete contact information phone e mail fax Purchase Order minimum is 20 00 inside USA and 25 outside USA Payment terms are Net 30 Days for established domestic customers Advance payment credit card or binding Irrevocable Letter of Credit add 350 fee in U S funds drawn on a U S bank is required for international orders i fF ie b 4 All shipments are F O B origin Discounts may be available for educational and multiple unit orders e All AutoMate Scientific manufactured products carry a one Warranty year Limited Warranty See Terms All new AutoMate Scientific manufactured products carry a one year and Conditions for more complete Limited Warranty against defects in materials and workmanship unless information otherwise stated in the product s manual or our Terms web page This Non AutoMate Scientific branded third party products a
152. re sold AS IS by AutoMate Scientific be Sanerelyanenidea hen written approval from AutoMate Scientific or which has been damaged warranty is only valid when the equipment is used for its intended purpose and does not cover equipment which has been modified without manufacturers standard warranties by abuse neglect accident or connection to incompatible equipment Non AutoMate Scientific branded third party products are sold AS IS by AutoMate Scientific but may be accompanied by their manufacturers standard warranties If you have questions about any manufacturers warranties that accompany such products please call or email us Warranty and technical support coverage may be suspended for institutions with outstanding balances over 30 days past due 126 www autom8 com Due to the nature of clinical laboratory applications AutoMate Scientific will NOT accept the return of any products which have been used with HAZARDOUS MATERIALS or harmful environment AutoMate Scientific equipment is intended for research use only We cannot be responsible for any harm resulting from use on humans e Returns Please inspect your shipping carton for damage upon receipt Shipments with visible damage should be noted on the shipper delivery slip prior to sign off and reported to AutoMate Scientific no more than 5 days after Returns e Unopened and unused products can generally be returned without cause within 30 days of receipt
153. reating and storing pro tocols We especially like the ability fo create new protocols and download them directly to the ValveBank it really couldn t be easier Dr Susan Abrahamson University of California Berkeley EasyCode Dialog Box Start Time o0 00 fos 40 End Time oa 01 54 00 Iterations Loop Options LJ Multi Channel hare ikemnei 2 O Eraggei O Erara 4 Enter start and stop times accurate to 0 01 sec e Use all ValveBank commands including multi channel looping and interface triggering Free your computer during experiments Transfer and run programs stored in the ValveBank which can be disconnected from the computer leaving it available for data acquisition 20 Expand the computing power of your ValveBank system a File Edit Display Experiment Program Help my us Drug Sequence 126 Psion on monn mn on Eana aaa m Drug Sequence 12B 1 B Channel 1 Bank 1 al Ga i Channel 1 Wash Buffer E l 00 00 42 000 Valve On Ch 12 i 0004 15 00 Valve Off au 00 05 42 00 TTL On ae i 00070 00 TTL Off 00 06 16 00 Valve On 00 09 06 000 TTL On i 00 12 06 00 TTL Off Channel 3 00 13 30 000 Valve Off 00 13 42 00 TTL On Galan 7 00 16 2400 TTLoff 00 16 24 0 End Of List See Channel 2 50 sM Thiopental ae Time Event 00 13 12 000 Valve On s 00 20 54 009 valve Off Iae 00 20 54 01 End Of List Channel 5
154. receive regular up to date information possible in this catalog however we cannot be EnS responsible for any errors or omissions AutoMate Scientific reserves the right to make changes to prices product descriptions specifications or other information without notice Refer to AutoMate Scientific s web site for the latest information No part of this catalog may be reproduced or transmitted in any form by any means without AutoMate Scientific s authorization Copyright 2006 2015 AutoMate Scientific Inc All rights reserved ValveBank ValveLink SmartSquirt Perfusion Pencil ThermoClamp BubbleStop and EasyCode are registered trademarks of AutoMate Scientific Inc AutoPrime Ready for Research BackStop ValveGuard Oxygen8 and are trademarks of AutoMate Scientific Inc TEFLON resin is a registered trademark of DuPont Macintosh is a registered trademark of Apple Computer Inc pClamp and AxoGraph are trademarks of Axon CNS Instruments Inc Windows is a registered trademark of Microsoft Corp Gimbal Piston Compact Sub Hertz Pendulum Precision Electronic Positioning System CleanTop and TMC are trademarks of Technical Manufacturing Corp Delta T Focht Chamber System 2 FCS2 Objective Heater System and Objective Controller are trademarks of Bioptechs Inc Other corporate names and trademarks are the property of their respective companies 800 998 MATE 127 AutoMate Scientific 812 Page St e Berkeley CA 94710 e USA
155. res include clear acrylic panels solid top triple track front section with a three piece sliding front panel Fixed Full Perimeter Enclosures A fixed welded steel structure that completely surrounds the table top to provide non isolated support for Faraday Cages raised rear shelves Plexiglas enclosures and other special fixtures Cannot be used with sliding shelves or support bars Precision Control Valves To minimize bottled air supply usage standard TMC height control valves have a small dead band resulting in a height return accuracy of 0 05 in 01 3 mm Precision valves control height to within 0 005 in 0 13 mm but have a slight constant leak To specify precision height control valves with a table add the letter P after the basic table model number 800 998 MATE Armrest Pads 00S 9 SATdVL AYOLVeOdY ai mM D Y Lu mM lt L gt ad COC lt L Qz Q m lt L Casters Breadboards Breadboard Specifications Core Steel honeycomb closed cell 0 010 in 0 2mm thick foil Core Shear Modulus 275 000 psi 19 300 kg cm2 Core Cell Size lt 0 5 in 2 3cm2 Core Density 13 3 b ft3 230 kg cm3 Top Skin 430 series stainless steel O 075in 2 mm thick Bottom Skin 400 series stainless steel O 075in 2 mm thick 96 Casters A set of four retractable casters with a total weight capacity of 1000 lbs 450 kg can be mounted to the
156. rized Ti S ER Ti S E ASI Flat Top amp Piezo Ti S ER TE200 Electronic FN1 Mechanical Nikon ThorLabs FN1 Fixed Narishige ThorLabs TS100 Mechanical DMI 3000 6000 DMIRB Mechanical DMI 3000 6000 Mechanical DMI 3000 6000 Electronic DMI 3000 6000 DMIRB DMIL Electronic DMI 3000 6000 DMIRB DMIL Mechanical DMIRB Mechanical DMIL Non Detachable N Nikon Ring Non Detachable ZK Zeiss K M zh Scan IM Stages ZK Zeiss K Non Detachable Nikon Stage Thorlabs MPM XYRS FN1 Nikon Ring Static O Olympus Ring ITS N PHYS24 Detachable UU Universal Holding Frame N A Attachable To Micromanipulator Slim Fixed Stage Non Detachable Non Detachable Non Detachable Slim ZK Zeiss K ZP Zeiss Plane 88 mm Ring N A Motorized 3 Plate Motorized 3 Plate Slim Motorized 3 Plate Leica Scanning 127X83 Prior Pro Scan ITK LMT200 ZK Zeiss K ZP Zeiss Plane 88 mm Ring N A ZK Zeiss K ZK Zeiss K ZK Zeiss K M zh Scan IM 130X85 ZK Zeiss K Detachable Object Guide ZM Zeiss M Non Detachable Square Adapter N A cy DEOD DO D SAA Sst 1 s JgWYHJ NOISNdH dd JDVISMIIND am NS aD AD ae NG ZS ESS S al re aS s CO lo oO Io INIA uA UW y ES UJ NS vI Microscope Upright NARISHIGE Upright Olympus THORLABS Upright Olympus Zeiss Configuration DM6000
157. rolled by a software interface it remains a conventional analog input output amplifier Thus it requires a separate system for controlling stimulus protocols and recording the output such as a digitizer and data acquisition software The Digidata 1440A digitizer and pCLAMP 10 data acquisition and analysis software from MDS Analytical Technologies work together to provide the most complete integrated solution Automatic telegraphing of the Axoclamp 9OOA amplifier requires pCLAMP 10 software and a Digidata 1440A or Digidata 1320 series digitizer 82 A complete microelectrode current clamp and volfage clamp amplifier Axoclamp 900A Amplifier The Axoclamp 9OOA amplifier is a complete microelectrode current clamp and voltage clamp amplifier useful for a wide range of intracellular microelectrode recording techniques Like it s predecessor the Axoclamp 2B amplifier the Axoclamp 9OOA amplifier has a wide range of functionality and has many enhancements that improve amplifier recording capability make the amplifier easier to use and help your experiments last longer The Axoclamp 900A amplifier has several modes of operation e Clamp two independent bridge amplifiers for voltage measurements e DCC discontinuous current clamp for accurate voltage measurements even when electrode resistance changes e TEVC high compliance two electrode voltage clamp for oocytes and mammalian cells e dSEVC discontinuous s
158. rtical adjustability e Solid aluminum construction e Flexible mounting design e Vacuum compatible versions available upon request MX512P Platform tr soe a he 62 A versatile platform for mounting a variety of hardware MX312P amp MX512P Vertical Platforms The MX312P is a versatile platform for mounting a variety of hardware at any level on an isolation table or platform A complete set of AS series spacers allows flexible vertical adjustment from 2 5 to 12 0 inches in 0 5 inch increments To adjust the height of the platform AS 1 5 and AS 2 0 series spacers are screwed together using 1 4 20 set screws The top and the base plates are attached to the four columns with 1 4 20 cap screws through the counterbored holes Smaller height adjustments are made by adding AS 5 and AS 1 0 spacers to the top of the longer AS spacers These are secured by the final attachment of the top plate with long 1 4 20 screws that go through the top plate and short spacers The MX312P base plate is equipped with a unique hold down pivot that allows secure single bolt mounting anywhere on the mounting surface The top mounting surface is 3 x 3 and has five 1 4 20 tapped holes for maximum mounting flexibility The top plate is also drilled and tapped with 1 4 20 holes on two sides These attachment points are designed to accept our MX APC T and MX APT T top plates These adapter plates allow the experimenter to remove and replace
159. runs on the US Windows Vista XP 2000 ME 98 32 bit operating systems as well as Macintosh OS X version 10 2 or higher OS 9 is not supported on a Power PC based system or OS 10 4 6 on an Intel based system A USB port is required to connect the MultiClamp 7OOB amplifier An additional USB port is required if the optional SoftPanel controller is used to control the amplifier Experimental Control and Data Acquisition Although the MultiClamp 700B amplifier is controlled by a software interface it remains a conventional analog input output amplifier Thus it requires a system for controlling your stimulus protocols and recording the output Our pCLAMP software and Digidata 1440A digitizer provide the most complete integrated solution Note pCLAMP 9 or higher is required for automatic telegraphing 81 lt Cc 7 gt lt U Q 2 lt L LJ OQ gt lt lt L Requirements Computer Control The Axoclamp 9OOA Commander program runs on US Windows Vista XP 2000 32 bit operating systems Two USB 2 0 ports are required to connect the computer to the Axoclamp 9OOA amplifier one for amplifier control and one for Monitor signal display on the computer screen for discontinuous modes An additional USB port is required if the optional SoftPanel controller is used to control the amplifier Experimental Control and Data Acquisition Although the Axoclamp 900A amplifier is cont
160. see ThermoClamp page 06 25 SmartSquirt Cryo vials 2 5 ml Qty 8 3 C lt 06 25c SmartSquirt Cryo vials 2 5 ml Bag of 100 25 02 01 08i Replacement SmartSquirt Valve 59 05 07 Colored SmartSquirt Tubing 1 16 i d per foot 4 07 04 c Replacement SmartSquirt Perfusion Pencil 4 channels 225 07 08 c Replacement SmartSquirt Perfusion Pencil 8 channels 225 37 Y aa Li m Z Q e Q Q vA mm eat lt L aN Li 6m lt L Y StageHand with magnetic base two arms and dual tube holder StageHand with magnetic base two straight arms locking ball joint and single tube holder Manifold holder manifold not included SH MH MS Single SH TH and dual SH DTH tube holders Right angle adapters SH OFF RAB amp SH RAB Regular arm SH STA and right angle SH RAA arm Interlocking arms hold other arms at 90 angles SH ISTA amp IRAA 38 Need a hand StageHands Kit 1 SH 1 includes the three black metal holders shown above StageHands Magnetic Tool Holders Magnetic base arms manifold and tube holders mount everything securely on your microscope stage for electrophysiology and perfusion The magnetic base of these different StageHands are designed to stick tightly to a steel ring around your perfusion chamber like our QuickStage or any steel surface nearby They include a 4 20 screw to insert into a non magnetic p
161. simultaneously with the Double In Vivo system Four reservoirs and two stopcocks are mounted for two independent wash buffer and fixative deliveries on the same ringstand www autom8 amp com Rodent Fixation Systems Ordering Information 11 140 In Vivo Perfusion System 140 ml mouse 174 11 04 140 Double In Vivo Perfusion System 4x 140 ml mouse 249 11 20s In Vivo needle 20 ga x 1 sharp straight needle for mouse Qty 4 1 11 20s cs In Vivo needle 20 ga x 1 sharp straight needle for mouse 19 Box of 100 11 24bf In Vivo needle 25 ga x 3 4 sharp butterfly needle for mouse Each 1 25 11 24bf cs In Vivo needle 25 ga x 3 4 sharp butterfly needle for mouse 50 Box of 50 11 117 Wax dissection tray Aluminum Tray for Mice 11 x 7 28 11 800 In Vivo Perfusion System 800 ml rat 199 11 18s In Vivo needle 18 ga x 1 sharp straight needle for rat Qty 4 1 11 18s cs In Vivo needle 18 ga x 1 sharp straight needle for rat Box of 100 19 11 20bf In Vivo needle 20 ga x 3 4 sharp butterfly needle for rat Each 1 25 11 20bf cs In Vivo needle 20 ga x 3 4 sharp butterfly needle for rat 50 Box of 50 11 139 Wax dissection tray Aluminum Tray for Rats 13 x 9 47 09 01 Swine cardiac pressurized perfusion system 630 U S Canada prices shown International prices add 15 Email or visit web store for latest prices 800 998
162. sliding shelves It may also be cantilevered Armrest Pads Adjustable leather forearm rests which fasten to the front support bar A new armrest pad is now available which fastens to the perimeter enclosure Sliding Shelves Shelves are made of wood with white plastic laminate covering all sides A metal bracket on the front edge of the shelf fits into the slot in the front support bar Shelves slide freely from side to side and are easily lifted off the support bars Built in stops prevent shelves from sliding out of slots When ordering sliding shelves you must order front and rear support bars Articulated ArmRest Our rugged leather Articulated ArmRest adds stability and comfort when delicate manipulations must be made without disturbing the isolated surface Mounts to front support bar or perimeter enclosures Raised Rear Shelf The 14 in 350 mm deep raised rear shelf mounts on the full perimeter enclosure and is the length of the corresponding table top The shelf is solidly supported 18 in 450 mm above the isolated surface with no direct noise transmission to the table top NOTE This shelf cannot be used with the Faraday Cage Sub Shelf For additional storage space a shelf mounted beneath the isolated table top is available and may be retrofit at any time Clear Acrylic Enclosures The clear acrylic enclosure provides protection from drafts and dust Custom configurations allow for acoustic and light protection Featu
163. solutions through the Perfusion Pencil tip and also deliver buffer for fast wash out Sophisticated circuitry will auto tune the ThermoClamp heating parameters based on your flow rates chamber and tubing to clamp the temperature and minimize over undershoot ThermoClamp Temperature Control System Ordering Information 03 11 LL ThermoClamp 1 1 channel with controller heated Pencil and sensors 1 295 03 14 x ThermoClamp 1 4 channel with controller heated Pencil and sensors 1 495 03 18 x ThermoClamp 1 8 channel with controller heated Pencil and sensors 1 495 x specify 100 250 or 360 um i d removable tip or ZDV for new Zero Dead Volume Pencil see right column 01 17b Rack mounting brackets ThermoClamp to 19 rack 20 03 02 1 Channel replacement heated ThermoClamp Perfusion Pencil 245 03 04 4 Channel replacement heated ThermoClamp Perfusion Pencil 445 03 08 8 Channel replacement heated ThermoClamp Perfusion Pencil 445 03 sensor Replacement bath thermocouple sensor 60 03 22 b ThermoClamp 2 controller with power cord 1 850 03 sub2 Substitute a ThermoClamp 2 for ThermoClamp 1 885 U S Canada prices shown International prices add 15 Email or visit web store for latest prices Manipulator not included 800 998 MATE Easy to Read or Record Pete ple Bence Crap etait T 6 e Large LCD 0 1 C temperature display
164. stage Pico is ideally suited for whole cell Vclamp and Iclamp and single channel patch planar lipid bilayer and synthetic nanopore recording as well as cellular electrochemistry Voltage Clamp and Current Clamp Clean Head Switching technology allows software controlled switching between voltage clamp and current clamp or between multiple feedback resistors without introducing any artifacts This enables one Pico to support a wide range of applications including whole cell single channel multi cell bilayers electrochemistry and current clamp Software Compatible with WinWCP by the University of Strathclyde jClamp by SciSoft Company TecellaLab software with Data Export to ATF tab formats SDK API available Other 3rd party software support Pico 2 Ordering Information Te Pico 2 Pico 2 Mini electrophys amp amp digitizer 7 900 Te Trigger Cable Optional cable to control external devices with WinWCP 500 Te BOB Optional break out box for 3rd party data acquisition system 820 Te jClamp32 jClamp Pico Software academic license call for non acad 1 600 www autom8 amp com with the smallest piezo micromanipulator Full featured manipulator with 20mm precise travel in all axes e High precision positioning with great finger tip feeling e Superior stability with drift free piezo drives e True approach angle and virtual 4th axis e Battery operated stand alone controller with rotary enco
165. techs exclusively offers opaque culture dishes which eliminates the unwanted ambient light background for fluorescence imaging The controller features a real time temperature display and fast learning curve to compensate for cooling due to surface evaporation while responding to temperature changes due to perfusion There is also an alarmed protection circuit to safeguard the cells and an internal reference for the user adjustable calibration The standard controller has a temperature range of ambient to 50 degrees C Extended ranges are available upon request Delta T dishes have 35mm O D and a 23mm central aperture The peripheral region of the dish is tapered to reduce the dead volume and the height of the dish is 6mm to allow better access for micro injection and micromanipulation The dishes are a hybrid of polystyrene plastic and Desag 263 glass The outer structure of the dish is available in opaque black or clear and come with a clear 0 5mm or no 1 5 glass coverslip bottom bonded to it The dishes are also available in a plain glass unheated version You will find the Delta T a reliable and indispensable addition to your microscope www autom8 com Delta T Stage Adapters and Accessories roller and Dish Accessories Alarm Led Colored Display Alarm Reset Button Mode Selection Red Setpoint Amber Stage Green Setpoint Orange Heated Lid Blue Imaging Red Heat Shock Power On Off Button BERRET
166. ter 26 QuickStage Heated Perfusion Chambers and Stage Adapters 27 Cool Solutions Peltier Heater Cooler 34 SmartSquirt Micro Perfusion 36 37 Perfusion Chambers 38 41 StageHands Magnetic Tool Holders 38 Oocyte Perfusion Chamber 40 Petri Dish Perfusion Chamber 41 Brownlee Instrumentation Amplifiers 44 NeuroLog Modular System 48 Hum Bug Noise Eliminator 51 DS2A DS3 amp DS4 Stimulators 53 DG2A Train Delay Generator 55 Brain Slice amp Tissue Chambers 57 59 Platforms Translators 60 Manipulators 66 Accessories 74 Piezo Switcher 76 Sutter Instrument 78 79 Manipulators 78 Micropipette Fabrication 78 Optical Instruments amp Microinjection 79 www autom8 com MultiClamp 80 WSLSAS NOILVXI4 LN3dOY Axoclamp 82 Axopatch amp Accessories 84 pCLAMP Software 88 DigiData Acquisition amp Ordering Information 89 Equipment Racks and Shelves 91 Laboratory Air Tables Breadboards amp Accessories 93 Faraday Cages 97 ze Active Tables Series 20 98 h TableTop Platforms Series 64 99 Active TableTops Series 66 100 O Bioptechs Heated Chambers 101107 rm DeltalT Open Dish Heater 101 5 DeltaT Accessories 103 L Perfusion Pump amp Software 106 Multi electrode Array 108 113 Alpha MED64 System 108 Alpha MED64 Probes 110 Alpha MED64 Mobius Software 111 Alpha MED64 Perfusion 113 SYOLYV INdINVW ChannelMAX Mini amp Twin ez gSEAL 116 ez g SEAL 119 uSlides amp Dishes 120 125 Biomicroscopy p
167. the premier Axon Instruments patch clamp amplifier incorporating the innovative Capacitor Feedback technology for single channel recording and resistive teedback for whole cell recording providing the best possible performance for single channel and whole cell patch clamping Convenient features include ZAP to rupture patches when going whole cell dual speed current clamp to allow faster current clamping in small cells Holding Command to set voltage commands in voltage clamp and current commands in current clamp and a choice of three gain settings on the dedicated current output for patch whole cell and loose patch modes The Axopatch 200B amplifier provides the lowest noise single channel recording available The amazingly low open circuit noise of 0 13 pA rms 10 kHz increases to only 0 145 pA rms when a patch pipette holder is attached to the headstage input and the pipette capacitance is fully compensated to eliminate capacitance charging transients The power of capacitor feedback technology is capacitor feedback at room temperature is clearly superior to resistive feedback technology cooled capacitor feedback is even better An unprecedented achievement in the field of ultra low noise recordings Unparalleled Performance Utility and Ease of Use Efficient controls for whole cell capacitance compensation a unique super charging form of series resistance compensation that complements the conventional correction form a
168. the specimen by providing a thermally conductive physical barrier between chilled fluid passing through the ring and the fluid surrounding the specimen This cooling ring is made of autoclaveable 304 stainless steel and provides the microscopist with a convenient and inexpensive method of reducing the temperature of specimens in Delta T culture dishes The cooling ring is supported on the stage adapter and translates along with the dish It is easily flipped out of the way to enable easy exchange of dishes in the stage adapter The Bioptechs Brain Slice Adapter is combined with the Delta T Culture Dish System to provide a convenient method of observing thick cut sections of brain or other tissue in a perfusable temperature controlled optical environment on an inverted microscope Perfusion ports are made of 304 stainless steel and are compatible with 1 16 tubing As with all Bioptechs Delta T Culture Dish Adapters the specimen is adjustable in the Z axis plane to accommodate the working distance of the objective Custom geometry adapters are available upon special order to accommodate specimens having unique geometry www autom8 com hs Delta T Stage Adapters The Bioptechs Delta T Stage adapter reads the temperature of the Delta T Dishes provides electrical contacts to power the dishes and supports the dish on the stage for translation All Delta T systems require a Stage Adapter These pictures are prov
169. tion cancellation TMC has produced a superior ultra quiet table in six degrees of freedom This advanced isolation technology may be combined with TMC optical tables and other TMC products as well as designed into equipment for OEM applications Featuring 10 dB of isolation vertically and 20 dB of isolation horizontally at 2 Hz a frequency at which other tables amplify vibration the Active Vibration Isolation Table is ideal for the most demanding applications in unusually severe vibration environments TMC Air Table Ordering Information TM 20 561 Series 20 Active Vibration Isolation Tables 16 740 Complete TMC product line available Call or email for part numbers U S Canada prices shown International prices add 15 Email or visit web store for latest prices www autom8 com lsolate floor vibration General Specifications Isolator Natural Frequency with unprecedented ease High ot portability and economy Horizontal 1 7 Hz Low Input Vertical 2 0 2 9 Hz Horizontal 2 2 3 5 Hz Isolation Efficiency 5 Hz Vertical 25 50 Horizontal 40 60 gt gJ E m O U U gt Tn C AO zx N l N m AU m N On F Isolation Efficiency 10 Hz Vertical 60 90 Horizontal 70 90 Gross load Capacity 3 isolators 1000 lbs 405kg 80 psi 4 isolators 1400 lbs 640 kg 80 psi Net load Capacity 3 isolators 250 Ibs 110kg 5 4 isolators 350 I
170. tioning versatility the top stage tilts at any angle through 360 and includes 22 mm of linear travel along the probe axis This feature plus 22 mm of X axis travel make the SD 160 ideal for axial cell probing The SD 160 has an MXC 45 mounted to the axis The MXC 45 s built in rotational stop allows easy electrode pipette replacement SD 160s come standard with the ABP R mounting plate The mounting plate design enables coarse positioning between platform mounting holes as well as 360 of coarse rotational positioning If rotation with a solid submicron level stop is required the MX RS rotation stage is designed to mount directly into the ABP R as well as the SD 160 800 998 MATE SD 130 Micromanipulator e Repeating probe holder e Combined coarse and fine control on X axis e Narrow space saving design e Resolution 5 um SD 160 Performance Specs ms ned i cer ay ah 6 k a q Le Ser el a e Rotatable mounting base e Axial approach on 4th axis e Repeating probe holder e Maximum load 2 lbs e Travel axis 0 86 inch 22 mm e Min controllable motion 10 um 6 A WY A lt am ES gt S gt O AD WY Y fi O lt L aA zZ lt L a gt Oo gt Z U U SD 10 Micromanipulator e 10 mm travel in all 4 axes e Dovetail mounting rail for easy removal or replacement e Maximum load 0 5 Ibs e Travel axis 0 39 inch 10 mm e Resolution 5 u
171. ts and service every day Imagine what you can do with the extra time you save Through our partnerships we bring you innovation One company cannot be good at everything This is why we have partnered with some of the best companies in laboratory instrumentation Our partners share our same values and passion for quality and excellence We work hand in hand to offer you the best possible selection of instruments for all of your research needs From data acquisition to brain slice chambers weve got it all thanks to teamwork At AutoMate Scientific we are proud to work with our partners and we stand by their products the same way we stand behind our own ALPHA MEIS 5 E Axon CNS SCIENTIFIC Molecular Devices BIOPTECHS Digitimer Innovative Devices For Research 4 Scientific fi Systems SUTTERINSTRUMENT beis DNI YWI AY IN 14 A JAO0JSIQ DNHA Alle gt JJ lt gt m OC OC Ep lt AYLSIWSHIOULIIA 14 Your new electrophysiology rig from AutoMate Scientific One stop One order for all your needs Faraday Cage Microscope Recording Manipulator s Inline Solution Heater Air Table Vacuum amp Trap Perfusion System Bubbler Perfusion Chamber Heated Stage Stimulation Manipulator s Platform amp Translator Complete Electrophysiology Rig State of the art Recording Workstation With over 22 years of experience designing
172. ttended solution delivery using a ValveBank8 AutoPrime Perfusion System Gravity Perfusion Systems Dr M Bruce Maclver M Sc Ph D Department of Anesthesia e Unattended solution switching Sind Ukey Avoid vibrations from switching valves by hand The ValveBank or ValveLink Medical Center controller handles all solution delivery so you can watch results not switch stopcocks Many special features are included for easy perfusion control Low noise circuitry e Increased reproducibility Valve switching is accurate to 0 01 seconds with programs up to 99 hours long under microprocessor control Consistent liquid delivery means better data e Pinch PTFE Inert and Lee Valves Choose between speed cost and ease of cleaning Several options are available for fittings and reservoirs e Manual and external valve control Flexible design Easy cleaning and calibration Slave mode valve operation controlled by your computer pClamp Pulse Acquire LabView AxoGraph etc 10 www autom8 com Valve Choices e Pinch Valves for Reduced Maintenance Easiest valves to clean and switch tubing Liquids never touch the valves Switches in 30 50 ms 1 32 i d silicone tube passes through and is pinched closed by solenoid activation All AutoMate Scientific valves include an individual indicator LED Our new aluminum enclosure keeps the valves dry from spills and offers luer lock ports for syringe reservoirs e PTFE Inert Valv
173. ual recordings this noise performance is achieved in part by cooling the input field effect transistors inside the headstage to well below O C Better noise performance is only part of the story The redesigned slim headstage improves electrode access to the preparation by making it easier to fit under your microscope We now include BOTH whole cell ranges previously available only in two separate headstages in one headstage The 200B amplifier includes all of the features of the 200A amplifier and a few more Enhancements include three recording configurations in a single headstage one patch and two whole cell ranges with capacitance compensation ranges of 100 pF and 1000 pF increased voltage and current command ranges to 1V for electrochemical measurements built in capacitance dithering capability for capacitance measurements and addition of series resistance compensation to the current clamp circuitry to improve performance Seal Test now provides current steps in current clamp mode as well as voltage steps in voltage clamp mode Leak Subtraction is now more www autom8 com sensitive in the most important resistance range The recording bandwidth has been doubled to up to 100 kHz Command and bandwidth ranges are larger Series Resistance compensation is now active in current clamp as well as in voltage clamp mode to enable bridge balance to be used A Superb Instrument The Axopatch 200B amplifier is the latest version of
174. ulators to be placed in one quadrant The SD 130 provides 38 mm coarse and 5 mm fine positioning travel in the X axis Iwo and a half turns of the coarse adjustment knob will fully retract the device in the X axis The assembly is fitted with an MXC 45 probe clamp for easy changing of electrodes or other implements Y and Z axis adjustments are accomplished with the fine adjustment screws mounted on the back of the device Additionally the Z axis adjustment screw may be configured with the knob on the top or the bottom whichever provides the best access The device angle is easily set by loosening the locking screw The RTC O 5 mounting base provides coarse rotational positioning and flexible mounting options of either base or post support Left or right hand models allow additional flexibility to suit your application The extra length dovetail stage of the X axis combined with the spring loaded pivot mechanism pat 6590723 of the other two axes allow for a high level of stability This allows for smooth and precise adjustments D 160 Full featured Manipulator Unlike stages with rack and pinion drive the SD 160 uses a spring loaded lead screw design against a solid stop ensuring drift free operation The SD 160 incorporates fast pitch screw drive positioning and smooth ball bearing motion in the X Y Z and axes for precision positioning Color coded knobs are used to identify axis location in low light conditions For added posi
175. useful for electrochemical measurements Holding Command The Holding Command of the Axopatch 200B has been enhanced over that of its predecessors with the addition of a X1 and X5 switch that allows you to choose either O 200 mV or O 1 V ranges An ON OFF switch can disable this control when an external command from a computer is used to establish the holding potential Pipette Capacitance Compensation In operation in both voltage or current clamp modes controls the magnitude and tau of two time constants Fast and Slow Cell Capacitance Compensation Compensate up to 100 pF B 1 or 1000 pF B 0 1 to allow recording from a large range of cell sizes Output Gain Ten gain settings spanning a 1000 fold range may be selected to scale the output to the most desirable level a range double that previously available 84 Bringing patch clamp noise down to unprecedented levels Axopatch 200B Amplifier The Axopatch 200B patch clamp offers the lowest noise patch clamp amplifier technology The open circuit amplifier noise in patch mode has been reduced to unprecedentedly low levels lt 15 femtoAmps rms below 1 kHz bandwidth lt 60 femtoAmps rms below 5 kHz bandwidth and lt 130 femtoAmps rms below 10 kHz bandwidth all measured with an 8 pole Bessel filter Noise is still low 145 femtoAmps rms below 10 kHz bandwidth with a pipette holder attached This translates into lower noise during act
176. uter and include eight programmable digital outputs for control of external devices such as stimulators pumps and recording devices Entire ValveBank programs can even be triggered by a single TTL pulse EasyCode Expand the computing power of your ValveBank Optional EasyCode software helps you program your ValveBank with a Mac or PC Windows using easy click and drag time bars Download your valve sequences into the ValveBank s memory with the included cable Run programs on the ValveBank which can be disconnected from the computer ValveBank or ValveLink8 2 Which controller is right for you Features ValveBank ValveLink8 2 CHANNELS A or 8 channels available 8 channels each USB network to 64 channels COMPUTER I O 8 digital in 8 digital out serial RS 232 8 digital in USB analog input event marker out DIGITAL INPUTS One pulse can start a ValveBank program One TTL input per valve or demultiplex and or TTL inputs each control 1 valve control up to 16 valves with 4 inputs PROGRAMMABLE Ves ValveBank keypad EasyCode software or Only using real time analog or digital outputs digital outputs from your data acquisition software from your computer data acquisition software Mac and PC EasyCode software to pre program Free Windows XP real time USB control and eee ValveBanks up to 16 ch networking software for up to 64 valves at once MANUAL CONTROL External keypad Front panel b
177. ution switcher uses a high speed piezo flexure guided nanopositioner to move pipette tips in solution switching applications It can move an electrode tip across a 5 um thick theta glass septum This system is ideal for measuring cellular response to drugs or other elements in solution Piezo movement can be triggered by either input from common recording amplifier equipment or by Siskiyou software via a LabVIEW interface that can control distance frequency and dwell time The piezo also includes the piezo amplifier box and the Input Shaping board which significantly reduces pipette tip vibration and can be programmed to virtually eliminate resonances from your specific system The piezo flexure guided nanopositioner is mounted to our ultra stable MX1640 crossed roller micromanipulator The MX1640 has 20mm of travel in all four axes The fourth axis can be set at any angle to select the desired approach angle This adjustable fourth axis incorporates our MXC 45 to allow rotation of the piezo pipette assembly up and out of the experiment for easy glass replacement The base of the MX1640 also has a built in coarse adjustment for rotation of the entire manipulator or can also be used with our MX RS rotation stage for more precise motion www autom8 com Rapid Pipette Sweeping Eliminating Unwanted Switching 3 m INN WY MN fa mi JJ Rapid theta glass transitions cause ringing of the mechanical structure Zoomed in vie
178. uttons MANUAL FEATURES 1 on master channel timed open TTL outputs 1 on master channel SPEED 10 milliseconds 1 millisecond Up to 12 watts 1 amp per channel 24 watts VALVE POWER A watts per channel or 8 watts total 2 amps total PRICE Higher Lower ValveBank Controller Ordering Information 01 01 ValveBank 4 programmable controller 1 095 01 08 ValveBank 8 programmable controller 1 695 BNC cable ValveBank to pbCLAMP Digidata et al td 4 BNC plugs to DB 25 ee seas 01 07 Rack mounting brackets ValveBank to standard 19 rack 20 01 06 ValveBank keypad 6 extension cable 20 Cables for Heka InstruTECH and LabView call U S Canada prices shown International prices add 15 Email or visit web store for latest prices www autom8 com Save time by purchasing disposable perfusion accessories from one source Be ti i eek E a kt a i uuu in i Perfusion Accessories Replacement syringes and reservoirs are available for all perfusion systems Luer lock 5 15 35 60 and 140 ml plastic syringe reservoirs are standard 35 ml glass syringes and PIFE Inert tubing are available with our Volatiles Pressure Upgrade for researchers with volatile agents in solution 800 ml beakers with luer lock fittings are available as replacements for the In Vivo Perfusion System r Rre ee iZ TT lal i er ee fii Hii I i ssurized res
179. w at target position White vibrometer real time position output Red HEKA amp current Correlation between physical position and HEKA amp output is evident The vibration of the theta glass pipette assembly causes repetitive switching of the patch clamp signal Same as graph above using patented vibration cancellation technology The undesirable rapid switching of the HEKA amp output red due to rapid recoil driven structural oscillations of the theta glass is eliminated Fast Piezo Ordering Information SD Piezo Fast Piezo Perfusion Switcher 9 300 SU QT120 90 7 5 Quartz Theta Tubing 50 pieces 1 20 mm o d 0 9 i d 75cm 80 SU BT 150 10 Borosilicate Theta Tubing 100 pieces 1 50 mm o d 1 17 i d 10cm 93 U S Canada prices shown International prices add 15 Email or visit web store for latest prices 800 998 MATE 77 Great service and great prices on Sutter Instruments 2 LLI 2 ad N Z ad LLJ gt N Sutter Instrument Sutter Instrument s expertise in electromechanical engineering and stepper motor control is evident in the sub micron resolution of their highly stable motorized and mechanical micromanipulators Whether your application requires the extensive programmable capabilities of the MP 285 the multiple manipulator control of the MPC 200 or one of their traditional manual style manipulators Sutter Instrument provides the total
180. w autom8 com Don t keep your cells waiting E Thumb nuts Magnetic plate Tool Tray Cell chamber Gasket Coverslip Heating insert Stage adapter Release two thumb nuts to change coverslips and clean the entire stack while the magnetic Tool Tray holds your tubes and electrodes for 1 SUsSEWVHI NOISNd dd JYVISMIING the next experiment See next page aD On for more photos DEDEDE D NW Ew aD om aD r ee i pe _ r _ rm CO leo DO TIN HIF w QuickStage microscope stage adapters and heated perfusion chambers e Integrated spill channel Shown with Tool Tray removed e Magnetic stainless steel tool platform Gasket and heaters visible with two studs for fast disassembly e For Nikon Olympus Zeiss and Leica upright amp inverted microscopes Ciencia AsO mi coveri under gasket The AutoMate Scientific QuickStage is designed to fit directly into your Olympus Nikon Zeiss K amp M Leica Siskiyou Scientifica Narishige Thor Labs ASI Prior Marzhauser or Sutter microscope stage or platform With a simple twist of two locking nuts the entire stainless magnetic Tool Tray lifts off with all of your magnetic tools still attached The underlying chamber and coverslip are then free for easy cleaning and replacement Optical 1 thickness cover slips provide a perfect imaging surface for upright or inverted microscopy The heated version
181. while buttons replicate single click mouse controls Headstages YASS AweosTacr Nimi sf fl The MultiClamp 7OOB amplifier comes standard with two identical but independent CV 7B headstages each of which contains both current to voltage and voltage following circuitry This design allows the user to rapidly switch between patch clamp recording and true high speed current clamp recording Thus with two headstages the MultiClamp 7OOB amplifier can perform the function of two patch clamps two current clamps or a combination of patch and current clamp amplifiers Furthermore two optional voltage follower headstages HS 2 type can be connected to auxiliary inputs to allow third and fourth point voltage recording The MultiClamp 7OOB amplifier supports up to four simultaneous headstages Iwo CV 7B headstages come standard the HS 2A headstages are optional The CV 7B headstage was designed for low noise and flexible recording features However some specialized applications require even more flexibility For example the rather large membrane capacitance in bilayer recording demands greater capacitance compensation The optional CV 7B BL headstage was developed for this purpose Another optional headstage the CV 7B EC was designed for large 2 V commands required during electrochemistry recording amperometry voltammetry 800 998 MATE Requirements Computer Control The MultiClamp 700B Commander program

perfusion systems temperature control amplifiers & stimulators data

Contents

Download Pdf Manuals

Related Search

Related Contents

perfusion systems temperature control amplifiers &amp; stimulators data

Contents

Download Pdf Manuals

Related Search

Related Contents

perfusion systems temperature control amplifiers & stimulators data