Precision Dilution System User Manual - Kanomax FMT, Inc.
Contents
1. Precision Dilution System User Manual 11 Version 1 4 Precision Dilution System Installation 11 Hook the tube holder onto the pump and snap down into place Connect the Drain Fitting Note FMT Inc does not supply the tubing for connecting the Dilution system to the water supply 1 Remove the red drain cap from the Drain fitting and discard Drain cap 2 Unscrew the 1 2 inch Swagelok nut and remove the stainless steel ferrules 3 Push the inch plastic tubing not provided into the Swagelok nut 4 Slide the smallest ferrule onto the tubing followed by the largest ferrule Warning The ferrules must be fitted in the correct order and orientation shown in the photo below Large ferrule Small ferrule Swagelok nut Precision Dilution System User Manual 12 Version 1 4 Precision Dilution System Installation 5 Push the end of the tube as far into the Drain fitting as you can 6 Slide the nut onto the ferrules engaging the threads Hand tighten until you feel resistance 7 Using a wrench tighten the nut up to 1 2 turn Warning Do not turn more than half a turn Too much torque will spin the bulkhead Connect to UPW 1 Remove the Flaretek nut from the Water Inlet and push out the cap Water inlet 2 Take a length of 1 4 inch PFA tubing not provided and flare one end 3 Push the nut you removed from the Water Inlet onto the other end of the tubing 4 Push the flared end of t2. Other solvents may be used with the Dilution System Please consult the factory for guidance 35C 5 o Maximum water FT S 35 C 95 F Pump tubing Inlet water pressure 138 to 481 kPa 20 to 70 psi Note a suitable water drain is required 15 35 C 59 95 F with shelf attached 321 mm 12 7 inches Flaretek is a registered trademark of Entegris Inc Swagelok is a registered trademark of Swagelok Company Teflon is a registered trademark of E I DuPont de Nemours and Company Inc Windows is a registered trademark of Microsoft Corporation Specifications subject to change without notice Precision Dilution System User Manual 25 Version 1 4 Precision Dilution System Appendix B Appendix B Peristaltic Pump Calibration Follow these instructions to check the pump calibration 1 Connect the peristaltic pump to a power supply 2 Press the power button to turn the pump on you should see the green indicator light The preset flow rate is 0 100 mL min but the pump displays the previous setting The green PUMP Flow rate diode confirms that the pump is configured for flow rate If the light is not on at the Pump Flow rate position press MODE until it is If the flow rate is not 0 100 use the settings A V arrows to adjust it Power bution RUN STOP button with green start stop the dilution indicator light Reset button reverses the flow Flow rate Settings buttons increase decrease the flow rate
3. MODE button scroll through Pump Flow rate diode different modes 3 If the peristaltic pump is connected to the inlet fitting on the Precision Dilution System disconnect the tubing Precision Dilution System User Manual 26 Version 1 4 Precision Dilution System Appendix B 4 Insert the inlet end of the injection tubing into a beaker or suitable collection vessel containing approximately 150 mL of UPW Note The tubing should touch the bottom of the container Tubing outlet end Tubing inlet end 5 Note the direction of rotation of the pump a clockwise rotation indicates flow into the dilution system and a counter clockwise direction indicates flow exiting the pump If necessary press RESET to set the flow out of the pump clockwise direction 6 Weigh a plastic 60 mL bottle not provided and record the weight to 1 100 gram 0 0001 g Insert the outlet end of the injection tubing into the 60 mL bottle Press RUN STOP to turn on the pump and start a stopwatch at the same time 9 Run the pump for approximately 40 minutes Press RUN STOP to stop the pump and stop the stopwatch at the same time Record the time the injection pump was operating Note There should be approximately 4mL of water left in the bottle 10 Remove the bottle and place the end of the injection tubing into a beaker of clean water to prevent the tubing from becoming contaminated 11 Weigh the 60 mL bottle and then calculate the flow
4. Water will flow from the Drain port on the back panel It will also flow from the SAMPLE INLET port through the Tygon tubing and into the beaker Check the Dilution System for leaks there should not be any If there are leaks check inside the instrument for loose fittings Note There is no potential for electric shock 8 Plug the supplied power cable into the power socket on the back of the pump 9 Press the Power button on the pump You should see the green power light and pump flow rate diode The display reads 0 100 mL min Display Flow Rate Diode Power Button Precision Dilution System User Manual 15 Version 1 4 Precision Dilution System Installation 10 11 12 Tubing to beaker 13 14 15 16 17 Press the Settings A arrow to increase the flow rate on the pump to between 3 and 4 mL min Allow the water to flow into the beaker for one minute to clean out the tubing Press the Settings V arrow to reset the flow to 0 100 mL min Confirm that the water pressure is still at 15 psi and the Dilution flow meter is still at 100 mL min Pull the positioning lever on the injection pump tube holder up until the flow through the Tygon tubing just ceases and then continue to lift it for two more clicks There should now be no water flowing into the beaker Positioning lever Fill the beaker with UPW and insert the Tygon tubing with the end of the tubing below the surface of the water Press RE
5. 58 11 05 58 11 06 58 11 07 58 11 08 58 11 09 58 11 10 58 11 11 58 11 12 58 11 13 58 11 14 58 11 15 58 11 16 58 11 17 58 11 18 58 11 19 58 11 20 58 11 21 58 11 22 58 11 23 58 Table 4 Time and particle counts for four PSL challenges gt 0 05 channel 634 627 633 647 630 622 669 643 647 666 175 Time 11 24 58 11 25 58 11 26 59 11 27 58 11 28 58 11 29 58 11 30 58 11 31 58 11 32 58 11 33 58 11 34 58 11 35 58 11 36 58 11 37 58 11 38 58 11 39 58 11 40 58 11 41 58 11 42 58 11 43 58 11 44 58 11 45 59 11 46 59 11 47 59 gt 0 05 channel 1 2 1 1 1 76 1961 2590 2724 2795 2848 2807 2830 2839 2780 2847 2851 2770 2796 2836 868 Precision Dilution System User Manual 31 Time 11 49 59 11 50 59 11 51 59 11 52 59 11 53 59 11 54 59 11 55 59 11 56 59 11 57 58 11 58 58 11 59 58 12 00 58 12 01 58 12 02 58 12 03 58 12 04 58 12 05 58 12 06 58 12 07 59 12 08 59 12 09 59 12 10 59 12 11 59 12 12 59 gt 0 05 channel 1 ei 1808 4506 4854 4914 4925 9045 4949 5026 5056 5034 5058 5015 5041 5150 1461 Time gt 0 10 channel 12 14 59 1 12 15 59 1 12 16 59 1 12 17 59 1 12 18 59 1 12 19 59 1 12 20 59 2502 12 21 59 7340 12 22 59 7711 12 23 59 1828 12 24 59 8037 12 25 59 7999 12 26 59 7985 12 28 00 8089 12 28 59 8008 12 29 59 7996 12 30 59 8213 12 31 59 8141 12 32 59 8073 12 33 59 8176 12 34 59 1164 12 35 59 0 12 36 59 1 12 37 59 1
6. Fluid Measurement Technologies Inc Precision Dilution System User Manual pict Sy pore ECISION DILUTION Part Number 1976138 Version 1 4 Precision Dilution System User Manual P N 1976138 Copyright Fluid Measurement Technologies Inc 2010 All rights reserved Address Fluid Measurement Technologies Inc 4106 Hoffman Road White Bear Lake MN 55110 3708 USA Phone Number 651 762 7762 Fax Number 651 762 7763 URL www fmtdevelop com The following is a history of the Precision Dilution System User Manual part number 1976138 Date and Changes Manual first release March 2010 Version 1 1 Updated procedures and photos reflect changes in PSL bottle size December 2010 Added Appendices B and C and minor edits Updated Appendices and minor edits Name change and extensive revision December 2014 All trademarks appearing in this manual are the property of their respective owners Fluid Measurement Technologies Inc may have patents or pending patent applications trademarks copyrights or other intellectual property rights covering subject matter in this document Providing you with this document does not give license to these patents trademarks copyrights or the intellectual property except as expressly provided in a written agreement from Fluid Measurement Technologies Inc Precision Dilution System User Manual 1 Version 1 4 Limitation of Warranty and Liability Fluid Measurement Technologie
7. SET to set the flow out of the pump it should now be flowing from the beaker to the pump Allow the system to rinse up for another 30 minutes to flush any contaminants out of the Dilution System Flush at a minimum flow rate of 100 mL min or the particle counter manufacturer s recommendations through the particle counter and the Dilution System Note It is helpful to flush through the tubing that will be used to connect the Dilution System to the particle counter and through the injection pump s Tygon tubing As necessary re adjust the Water Pressure Regulator to 15 psi and the flow meter valves to the flow rate recommended by your particle counter s manufacturer See following instructions Press RUN STOP to stop the pump Precision Dilution System User Manual 16 Version 1 4 Precision Dilution System Installation 18 Using the provided wash bottle wash the end of the injection tubing with UPW water to remove any potential contaminants Note The tubing will eventually be inserted into the PSL standard and MUST be clean or it will contaminate the standard Connect a Particle Counter I Turn off the water supply Warning If you do not turn off the water you risk flooding your work area If you do not want to repeatedly turn off the water supply to connect and disconnect a particle counter install isolator valves over the DILUTED SAMPLE SUPPLY and DILUTED SAMPLE RETURN ports Note FMT Inc does not provide isolat
8. This Manual ascita egal ne aid caii dia died ca eta iii net 4 TE Lig re Audien ney ein mapa ala pui i Oa aa aa n E 4 Scope Or User Mana nn ea tie 4 DEfITIL IO 10 sat rr ee eee rene ae cree meer tre ert re err ai ati i en 4 Safety and Handhine Procedures nd ina a r 5 DAC SIENA LS pe ee E EE ot nr lata oaia CN ne 5 WY AIS le nee ad E 5 Installing the Precision Dilution System ses 6 Equipment Need RNA dents 6 IP EOCECIUEEE Sosi dag 0 ab 0 la seam acess Whe A eases e e cad 6 PLP DEON IM te IE saca pala aaaoae aa nn arda oaia dal ea d ri aa ue to nada load 6 Precision Dilution Sy Stes SCM Sra tC soccer da pi ala 0 as T 7 Unpacking and Setting Up the Precision Dilution System ss 7 Unpack the Precision DUO SS TEI aice ete area d nid Ann Mit 7 Attaehthe eristal IC PAIR pietii teapa eee au aa ea 0 a aaa la alee Get 8 Connect the Prat POTE secon Doo d aia e ae a la BE ttea a i 12 CONNECTION se GR aaa tal oa a o za ll tau ut aa in ad n ua 13 Turn on UPW Flush the System Power On and Tension the Tubing s es 14 Connecta Particle Counter piss tice nus 17 Operating the Precision Dilution System ss 19 Peronin DUNOIS passan alani et todo tat da etala 19 Pluton Cala tons ada Ra a aaa ui Bai at atat dea ial dat Eta 21 Shutting Down the Precision Dilution System ss 23 Troubleshoo no a Tin 24 selecting the Cored Pump Ubn SR ne en terne ue 24 Appendix A Specifications si ces pa catia a ad lau i Sl a dl ac 25 Appendix B Peristaltic P
9. Version 1 4 Precision Dilution System Appendix D Appendix D Calibration Certificate Example An example of a Certificate of Calibration is shown below with FMT lot numbers A brochure for the nanoparticle standards offered by Fluid Measurement Technologies can be downloaded at http www fmtdevelop com uploads 3 5 4 5 3545567 nanoparticlestandards b rochure pdf The FMT web site www fmtdevelop com also has a copy of a paper entitled Verifying the Calibration of Optical Particle Counters below 100nm Blackford Van Schooneveld and Grant presented at the UPW Executive Forum Phoenix November 2010 This paper details the procedure for making PSL suspension of known concentration Figure 4 Example of a Certificate of Calibration for 60 nm PSL Spheres Se 3 ma tes pE r gry FF F N 4 Ta 4 S 4 lt r N wd yu lt Fluid Measurement F Technologies In Certificate of Calibration NIST Size Traceability from Duke Scientific C orp 60 nm PSL Spheres DESCRIPTION The particle size standard detailed below provides an accurate and traceable size calibration for particle size analysis They are part of a series of monodisperse polymer microspheres with calibrated mean diameters traceable to the Standard Meter through the National Institute of Standards and Technology NIST Diameters from a nominal 60 nanometers nm to a nominal 100 micrometers nm are available as calibrated by photon correlation spectrosc
10. d especially through the OPC ports Release tension on the injection pump tubing and allow CDA N2 to purge the injection tube Do not re tension the tubing until you are ready to operate the system again To shut down the Precision Dilution System completely follow these instructions 1 9 Flush any PSL from the dilution system by running UPW water through it at a high flow rate for one minute At the same time run the injection pump backwards at a flow rate of 3 4 mL min with the beaker in place to collect the water Press the power button to turn off the peristaltic pump Turn off the water supply Turn the Water Pressure Regulator counter clockwise and open the three flow meter valves Release the tension on the injection tubing by releasing the tensioning lever on the injection pump Attach the supplied bypass tubing to the DILUTED SAMPLE SUPPLY and DILUTED SAMPLE RETURN ports Using the supplied CDA N2 Adapter Fitting blow out the Dilution system with CDA or Nitrogen at 5 10 psi for 24 hours to completely dry out the instrument Disconnect all tubing and cap off all inlets outlets If you are shipping the instrument disassemble it by reversing the instructions in the Installation section of this manual Precision Dilution System User Manual 23 Version 1 4 Precision Dilution System Troubleshooting Troubleshooting Selecting the Correct Pump Tubing Size The default size for the pump tubing is 0 13 mm The Pr
11. ecision Dilution System is supplied with a pump tubing size of 0 64 mm This size of tubing has been entered into the pump s memory at the factory However customers have reported that as a result of a power outage or incorrect shut down procedures the Ismatec pump has reset to the default pump tubing size resulting in the pump running much faster than usual Follow these instructions to check that the correct tubing size is entered into the pump s memory 1 PA gt Switch the pump off and wait for five seconds While keeping the settings Y button pressed switch the pump on wait a few seconds and then release the settings Y button The display should read C4CL the setting for Cycles Press the settings A button 7 times The display should now read tubE Press OK The display should read 0 64 If this is the case the pump has retained the correct tubing size and no adjustment is necessary Press escape twice to revert to normal pump operation If the display reads the default setting of 0 13 press the settings A button 7 times to scroll through the available tube sizes 0 13 0 19 0 25 0 38 0 44 0 51 0 57 0 64 When the display reads 0 64 press escape twice to revert to normal pump operation Precision Dilution System User Manual 24 Version 1 4 Precision Dilution System Appendix B Appendix A Specifications Working fluid Ultrapure Water for diluting PSL and Colloidal Silica concentrations Note
12. h as the PMS UDI50 which has a flow rate of 1000 mL min increase the flow through the high flow rate flow meter to 900 mL min The flow through the low flow rate flow meter should be 100 mL min for a total flow of 1000 mL min The Dilution flow meter should read 0 mL min Precision Dilution System User Manual 18 Version 1 4 Precision Dilution System Operation Operating the Precision Dilution System Performing Dilutions PSL verification standards are available from Fluid Measurement Technologies Inc in 8mL capacities PSL sizes are displayed on the top of the verification standards boxes We recommend that you begin with the smallest PSL standard sizes in the box you re using and progress to the larger PSL sizes Each box contains 8 bottles of one size PSL Standards MOD Con Gn PSL concentration ds 107 per mL Concentration Accuracy 10 Expraton Date 1 December 2014 sea bottle for Loi a Follow these steps to perform dilutions 1 Begin with a bottle taken from the box with the smallest PSL standard size 2 Rinse the inside of the cap provided that has a hole in the center with UPW from the rinse bottle Warning You must flush the cap with clean water before placing it on a verification standards bottle The verification standards bottles are filled almost to the top therefore the cap collects PSL spheres as it is moved from bottle to bottle Flushing with clean water ensures that PSL sphere
13. he tube onto the Water Inlet fitting Flared tubing Precision Dilution System User Manual 13 Version 1 4 Precision Dilution System Installation 5 Hand tighten the PFA nut over the Water Inlet PFA nut Turn on UPW Flush the System Power On and Tension the Tubing 1 Remove the Flaretek nuts from the DILUTED SAMPLE SUPPLY and DILUTED SAMPLE RETURN ports 2 Take the provided OPC Bypass tubing and attach to DILUTED SAMPLE SUPPLY and DILUTED SAMPLE RETURN ports Note Instead of the provided OPC Bypass tubing use any preferred PFA tubing that your procedures for OPC calibration require Bypass Tubing 3 Check that the positioning lever on the injection pump tube holder is rotated clockwise to the down position Then place the end of the Tygon tubing into an empty beaker 4 Turn the WATER PRESSURE REGULATOR counter clockwise to close the regulator Water pressure regulator Precision Dilution System User Manual 14 Version 1 4 Precision Dilution System Installation 5 Turn on the water 6 Partially open the needle valve beneath the Dilution flow meter Two turns in a counter clockwise direction should be sufficient Dilution flow meter needle valve 7 Turn the Water Pressure Regulator in a clockwise direction At the same time turn the dilution valve to maintain a flow of 100 mL min Slowly increase the water pressure to read 15 psi Water should now flow through the Precision Dilution System
14. in irrevocable damage to the instrument Note Notes are used to indicate important information about the Precision Dilution System Please familiarize yourself with the following warnings before operating the Precision Dilution System The Precision Dilution System must be used following the manufacturer s specifications or safety cannot be guaranteed All service work must be performed by trained qualified technicians Only qualified service technicians should remove the Precision Dilution System cover Follow the instructions for all inlet and outlet connections incorrect connections will cause the Precision Dilution System to malfunction Any air or nitrogen supplied to the Precision Dilution System must be filtered particle free dried oil free and regulated at 138 kPa 20 psi When not in use for dilutions leave the Precision Dilution System running with water flowing through it at the lowest flow rate 78 61 to prevent potential contamination If standing water is allowed to stay stagnant in the system you risk bacterial contamination The Precision Dilution System must be drained before it is shipped Do not ship an undrained Precision Dilution System doing so will invalidate the warranty Do not subject an undrained Precision Dilution System to freezing temperatures Doing so will damage the instrument and invalidate the warranty Precision Dilution System User Manual 5 Version 1 4 Precision Dilution Syste
15. ing concentration of 4x107 PSL mL injecting at 0 1 mL min into a total flow rate of 500 mL min gives a final concentration of 8 000 PSL min 0 1x4x10 500 8000 Precision Dilution System User Manual 21 Version 1 4 Precision Dilution System Operation For a PMS UDI50 the maximum concentration recommended by the manufacturer is 10 000 particles mL To achieve the challenge concentration of 8 000 particles mL at a dilution of 1000 mL min the flow rate for the UDI50 the injection speed must be increased to 0 2 mL min In this instance an 8 mL verification standards bottle will allow a total of 40 minutes of calibration time Results for a UDI50 challenge are shown in Appendix C see page 29 Precision Dilution System User Manual 22 Version 1 4 Precision Dilution System Shut Down Shutting Down the Precision Dilution System Warning Do not leave the Precision Dilution System full of water with no flow through the instrument If you do you run the risk of bacterial growth and serious contamination If you will not be operating the Precision Dilution System for 24 hours or more you have two options Leave the Dilution System flushing with UPW at a total flow of 300 mL min allow a flow of 100 mL min through each of the flow meters Then run the injection pump in reverse flow out of the dilution system at the lowest flow rate Purge the system completely with CDA or filtered N2 until all residual water is remove
16. ize rinse the Precision Dilution System for 5 10 minutes to obtain a stable background During the rinse run the injection pump in reverse mode press the Reset button so that water flows from the dilution system into a beaker This will remove any PSL particles from the Tygon tube 9 When the rinse is finished press Reset on the pump 10 Remove the cap from a bottle in the box of standards for the next size 11 Remove the cap with a central hole from the verification standards bottle you used for the previous dilution Rinse the cap see step 2 and place it on the next standard bottle 12 Wash the end of the injection tubing with UPW water to remove any potential contaminants Push the injection tube through the hole in the verification standards bottle cap until it reaches the bottom of the bottle 13 Repeat steps 6 11 until you have used a bottle from each of the verification standards sizes Dilution Calculations Ideally you should challenge a particle counter at 80 of the maximum concentration recommended by the manufacturer At an injection flow rate of 0 1 mL min the 4mL verification standards bottle can be used for 40 minutes However 15 minutes is sufficient time to obtain a stable reading on the particle counter Therefore one 4 mL standard bottle can be used for validating the calibration of two particle counters Example The PMS M50 will require a target concentration of 8 000 particles mL Therefore a start
17. m Installation Installing the Precision Dilution System Follow the instructions in this section of the manual to install the Precision Dilution System Equipment Needed Va inch PFA tubing Ya inch flaring tool 1 inch plastic drain tubing Flaretek nuts Va inch wrench Philips driver 2 Stopwatch Scale with readability to 0 01 grams Glass or plastic beaker 125mL plastic bottle Procedures Refer to the flow diagram on page 7 as you install and operate the Precision Dilution System Following is an overview of the steps required to get your Dilution System up and running de 2 S 4 Attach the peristaltic pump Connect the Drain fitting Connect to UPW water Turn on the UPW flush the system power on and tension the injection tube Connect to a particle counter Please read the detailed instructions for each step beginning on page 7 before you set up the Dilution System Approximate Time Completing all the assembly procedures will take approximately 30 minutes Note The Dilution System must be flushed for 30 minutes before use to remove any contamination Precision Dilution System User Manual 6 Version 1 4 Precision Dilution System Installation Precision Dilution System Schematic Figure 1 Precision Dilution System Flow Schematic Precision Dilution and Injection System Optical Particle Counter Mixing Dilution OPC Chamber Flow rate Flow rate OPC 400 mL min 100 mL min Pre
18. on Verification for a PMS UDI50 In situ Optical Particle Counter This procedure demonstrates how a calibration verification of an optical particle counter with the Precision Dilution System Model 6138 can replace a routine factory calibration A Precision Dilution System Model 6138 was connected to a UDI50 with the following data logging conditions Data logging began after a 30 minute rinsing period All data was logged using PMS Facility Net software Data collection was set for a 60 second counting time interval All channels were logged as cumulative counts greater than their respective size channels Procedure The UDI50 was challenged with four PSL verification standards at nominal sizes of 60 70 80 and 100 nm Figure 4 in Appendix C shows an example of a PSL data certificate for the additional data on the actual PSL NISI certified size from Duke Scientific The initial concentration for each size of FMT PSL suspension is carefully adjusted to 4x10 PSL particles mL The Precision Dilution System was configured to deliver a concentration of 8 000 PSL particles mL to the UDI50 Each PSL challenge lasted for 15 minutes There was a 10 minute clean up time between each challenge Results Table 4 shows the results of the calibration verification in time and counts Figure 3 is a graph showing the results in a log of particle mL versus time Data for the 60 70 and 80 nm challenges are shown as greater than the 0 05
19. opy PCS transmission electron microscopy TEM The aqueous medium has been prepared to promote dispersion and reduce clumping of the particles The certified mean diameter is traceable to NIST The concentration is verified using an UltraFine Atomization Scanning Mobility Particle Sizing UFA SMPS system Duke Catalog Number 3060A Nominal 60 nm Duke Packaging Lot 35017 Duke Expiration Date April 2012 FMT Lot 1018 FMT Expiration Date July 2011 Certified Mean Diameter 59 nm 2 5 nm Standard Deviation 9 9 nm Coefficient of Variation 16 8 Hydrodynamic Diameter 58 65 nm PCS Concentration Data Concentration 4 x 107 PSL spheres per mL at 60nm Accuracy 10 Lot to lot variability 5 Concentration data valid through July 2011 Physical Data Microsphere Composition Polystyrene Microsphere Density 1 05 g em3 Index of Refraction 1 59 589 nm Approximate Concentration 0 00000043 as solids No additional Surfactant added Store at room temperature Du Avoid exposure to direct sunlight David Blackford Presid Precision Dilution System User Manual 32 Version 1 4
20. or valves Remove the Bypass tubing from the DILUTED SAMPLE SUPPLY and DILUTED SAMPLE RETURN ports on the front panel Attach a particle counter following the manufacturer s instructions To gather data connect the particle counter to a data logging device following the manufacturer s instructions Turn on the water Recommended flow rates for particle counters vary The following instructions use a PMS M50 particle counter with a recommended flow rate of 100 mL min as an example The readings should be taken from the center of the metal balls You can use an external flow meter or the flow meter built into your particle counter to adjust the flow rate through the particle counter Precision Dilution System User Manual 17 Version 1 4 Precision Dilution System Installation Dilution flow flow meter High flow flow meter Low flow flow meter 6 For a low flow rate particle counter such as the PMS M50 lt 100 mL min flow rate the total flow through the 100 mL min flow meter and the Dilution flow meter should be 500 mL min The flow rate through the 100mL min flow meter should be at the particle counter manufacturer s recommended flow rate and the remaining flow should pass through the Dilution flow meter Note There should be no flow through the 1000mL flow meter Changes in the total flow rate will affect the water pressure which you may have to adjust to 15 psi 7 For a high flow rate particle counter suc
21. rate f _ weight mg a time min If the pump calibration is accurate the flow rate should be 2 of 0 100 mL min assuming the water has a density of 1 00 g mL If not use this calculated value to apply a correction to the peristaltic pump calibration as detailed in the worked example below a A worked example The pump ran for 51 minutes and the difference in bottle weights was recorded as 4 9847g Using the equation above the flow rate is calculated to be 0 0977mL min i e the pump is running 2 3 slow Precision Dilution System User Manual 27 Version 1 4 Precision Dilution System Appendix B b To permanently enter a flow rate correction into the peristaltic pump press the CAL MAX button The Display will now flash Using the Settings Y button reduce the number displayed to 0 098 Press the CAL MAX button again to enter the new calibration value The display stops flashing and displays 0 100 Note the display will only flash for a few seconds to allow the new flow rate to be entered c To check the new flow rate calibration repeat the steps 8 11 d In this worked example the pump was run for 46 minutes and the weight change in the bottle was 4 5895g Using the formula this corresponds to a flow rate of 0 0998mL min i e the pump is running 0 2 slow which is within the calibration requirements Precision Dilution System User Manual 28 Version 1 4 Precision Dilution System Appendix C Appendix C Calibrati
22. s FMT Inc offers a full 12 month repair or replacement warranty on all products sold Warranty covers defects resulting from design or manufacturing If any such product proves defective during this warranty period FMT Inc at its option either will repair the defective product without charge for parts and labor or will provide a replacement in exchange for the defective product This warranty is offered on a return to factory basis with return shipping expense expressly at the cost of the customer FMT Inc will pay the return shipping on any materials repaired or replaced Any repaired or replaced item assumes the remainder of the initial warranty period FMT Inc warrants only to the extent of replacement or repair of our products and does not assume any responsibility for consequential damages No warranty is offered on accounts with past due balances In order to obtain service under this warranty Customer must notify FMT Inc of the defect before the expiration of the warranty period and make suitable arrangements for the performance of service The Customer shall be responsible for packaging and shipping the defective product to the service center designated by FMT Inc with shipping charges prepaid This warranty shall not apply to any defect failure or damage caused by improper use or improper or inadequate maintenance and care FMT Inc shall not be obligated to furnish service under this warranty a to repair damage resulting from a
23. s of one size do not contaminate a bottle containing a different size NC Cap with central hole Precision Dilution System User Manual 19 Version 1 4 Precision Dilution System Operation 3 Remove the cap from the first standard bottle you want to use and replace with the cap with a hole in the center 4 Wash the end of the injection tubing with UPW water to remove any potential contaminants Warning The tubing will be inserted into the PSL standard and MUST be clean or it will contaminate the standard You should perform this procedure every time you use a different verification standards bottle 5 Push the injection tube through the hole in the cap until the tube touches the bottom of the bottle Tube placed in standards bottle Precision Dilution System User Manual 20 Version 1 4 Precision Dilution System Operation 6 Calculate the dilution rate and pump speed see example of such a calculation in Dilution Calculations on page 21 7 Perform a dilution with the standard In this instance an 8 mL verification standards bottle will allow a total of 40 minutes of calibration time However 15 minutes is sufficient time to obtain a stable reading on the particle counter Therefore one 8 mL standard bottle can be used for validating the calibration of two particle counters Results for a UDI50 challenge are shown in Appendix C 8 Before beginning the next challenge with a bottle from the box of the next PSL s
24. ssure Peristaltic Pump Compressed Air Actuated Regulator UPW Inlet B Concentrated PSL suspension Unpacking and Setting Up the Precision Dilution System Unpack the Precision Dilution System To unpack the Precision Dilution System follow these instructions 1 Carefully remove the Precision Dilution System and all its components from the packing case Save the original packing materials for use when returning the Dilution System to FMT Inc 2 Check that all the items listed in Table 1 were included If any of the items are missing or damaged please call Fluid Measurement Technologies Inc at 651 762 7762 Precision Dilution System User Manual T Version 1 4 Precision Dilution System Installation Table 1 Precision Dilution System Packing List Part Number Quantity 1026138 Precision Dilution System Main Body and Mounting Bracket 1021000 Ismatec Precision Metering Peristaltic Pump Cassette Cartridge Cassette Cartridge with Pressure Lever and Instruction Manual Main body Power cord Mounti Peristaltic nk pump Tygon tubing with inlet fitting Attach the Peristaltic Pump The peristaltic injection pump supplied with the Dilution System is preset to a flow rate of 0 100 mL min to within 2 and should not require re calibration upon initial installation Check the pump calibration once a year after the initial installation following the procedures described in Appendix B To a
25. ttach the pump follow these instructions 1 Remove the plug from the SAMPLE INLET 2 Attach the inlet fitting on the supplied Tygon tubing to the SAMPLE INLET and finger tighten The fitting should be snug Precision Dilution System User Manual 8 Version 1 4 Precision Dilution System Installation Sample Inlet 3 Lay the main body on its side on the workbench Using a Philips screwdriver unscrew the four screws used for the mounting bracket Bracket mounting screw 4 Screw the mounting bracket in place using the screws you removed and return the main body to a vertical position 5 Remove the two screws from the left hand side of the peristaltic pump and reserve for use in step 7 Precision Dilution System User Manual 9 Version 1 4 Precision Dilution System Installation 6 Place the pump on the mounting bracket 7 Insert the screws you removed in step 5 onto the side of the bracket to hold the pump securely in place Pump mounting screw 8 Remove the front tube holder with adjustable pressure lever from the pump by pushing in the tab to the left and lifting the holder up Front tube holder Tab Precision Dilution System User Manual 10 Version 1 4 Precision Dilution System Installation 9 Take the supplied Tygon tubing and slot it into the tube holder Place the orange tube positioner on the far right of the tubing against the tube holder Orange tube positioner
26. ttempts by personnel other than FMT Inc representatives to install repair or service the product b to repair damage resulting from improper use or connection to incompatible equipment or c to service a product that has been modified or integrated with other products when the effect of such modification or integration increases the time or difficulty of servicing the product THIS WARRANTY IS GIVEN BY FMT INc WITH RESPECT TO THE LISTED PRODUCTS IN LIEU OF ANY OTHER WARRANTIES EXPRESS OR IMPLIED FMT AND ITS VENDORS DISCLAIM ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE FMT RESPONSIBILITY TO REPAIR OR REPLACE DEFECTIVE PRODUCTS IS THE SOLE AND EXCLUSIVE REMEDY PROVIDED TO THE CUSTOMER FOR BREACH OF THIS WARRANTY FMT AND ITS VENDORS WILL NOT BE LIABLE FOR ANY INDIRECT SPECIAL INCIDENTAL OR CONSEQUENTIAL DAMAGES IRRESPECTIVE OF WHETHER FMT LLC OR THE VENDOR HAS ADVANCE NOTICE OF THE POSSIBILITY OF SUCH DAMAGES Service Policy Knowing that inoperative or defective instruments are a detriment to our customers satisfaction our service policy is to give prompt attention to any known problems If you discover any malfunction of the Precision Dilution System contact FMT Inc at 651 762 7762 USA If you are outside of the USA please call your authorized distributor Precision Dilution System User Manual 11 Version 1 4 Table of Contents Table OF Contents iene ca aie ada Bula arin E ul a ul ala 3 About
27. um channel Data for the 100 nm challenge is shown for the gt 0 10 um channel because at 100 nm the PSL spheres are large enough to pass through the edge of the laser defined viewing volume and cause false counts in the gt 0 05um channel Calculate the detection efficiency percentage for each challenge Table 3 shows the detection efficiency percentages for this UDI50 Precision Dilution System User Manual 29 Version 1 4 Precision Dilution System Appendix C Table 3 Measured detection efficiencies for each of the 4 challenges PSL Challenge size nm UDI50 detection efficiency II 79 These detection efficiency percentages can now be used as benchmark data for verifying the calibration of this particular PMS UDI50 If this PSL challenge procedure is repeated in 6 or 12 months an acceptable limit on the change in detection efficiency such as where each detection efficiency value drifts by no more than 15 can be used to verify that the particle counter remains within calibration and does not need to be calibrated at the factory Figure 3 Log of particle mL versus time PMS UDI50 Optical Particle Counter Response to 60 70 80 and 100nm PSL Challenges 10000 1000 5 2 100 O O LO OQ 10 1 11 00 00 11 20 00 11 40 00 12 00 00 12 20 00 12 40 00 Time Precision Dilution System User Manual 30 Version 1 4 Precision Dilution System Appendix C Time 11 00 58 11 01 58 11 02 58 11 03 58 11 04
28. ump Calibration ss 26 Appendix C Calibration Verification for a PMS UDI50 In situ Optical Particle COUNTER sn sep datata 6 alai aia iu abona Catolic aa ai aia abat 29 PPO CS UII E cae o Fas sie daia dai tg ea ada aa a a d ala a da ad la 29 PRG SUES bape doo fas Aia daca oase le dica aan pat da aa a dai 05 d all dota 29 Appendix D Calibration Certificate Example ss 32 Precision Dilution System User Manual 111 Version 1 4 Precision Dilution System About the Manual About This Manual Intended Audience The Precision Dilution System User Manual is intended to be used by qualified trained certified personnel including technicians and engineers Scope of User Manual This User Manual contains detailed instructions for the set up of the Precision Dilution System Definitions PSL Polystyrene Latex UPW Ultrapure Water psi pounds per square inch mL min milliliters per minute Precision Dilution System User Manual 4 Version 1 4 Precision Dilution System Safety Safety and Handling Procedures When working with the Precision Dilution System Do not remove any parts from the instrument unless this manual tells you to do so Safety Signals Warnings The following warning symbols and labels are used in the documentation Follow the procedures described in this manual to use the instrument safely Warning Warnings are used to indicate that unsafe use of the instrument could result
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