ABI PRISM® 310 Genetic Analyzer Clear Memory Reset Procedure
Contents
1. Page 4 of 9 Clear Memory Reset Module Applied Biosystems BS ene Calibration Sticker NOTE The values presented here are NOT representative of your instrument They are here for reference purposes only Make sure to record and use values for your instrument CCD Pixel Position X O SSS Manual Control B Function Module mone NOTE The number here is for reference only Your instrument may be different CCD Pixel Position Y SSS mana tat E Function CCD Pixel Position w Module Some O O 2222 NOTE The number here is for reference only Your instrument may be different Page 5 of 9 Clear Memory Reset Module XS Applied Contacting AB Technical Support By Telephone 1 800 831 6844 By Internet http www appliedbiosystems com support Then click on Frequently Asked Questions and then the Ask a Question tab By E mail AB Technicalsupport appliedbiosystems com Gel Pump and Tray Buttons Manually Launch Data Collection sii Double click on the shortcuts on the FJ computer desktop ee Alternatively navigate via the Apple aa g AEI Fram 310 Menu Start Menu 447 Drp E 270 Oo Eao Collection bat On the Macintosh computer On the PC Page 6 of 9 Clear Memory Reset Module Applied Biosystems AS Reset Button Syringe Pump at Home Position Page 7 of 9 Clear Memory Reset Module pplied Biosystems Syringe Pump on Plunge2. Recording Calibration Values 14 Ensure that the Data Collection software is running If not manually launch it 2 From the Window menu select Manual Control which opens a new window From the Function drop down list select and record the following values e CCD Pixel Position X horizontal e CCD Pixel Position Y vertical e Syringe Max Travel e Syringe Gel Pump Force 4 From the Function drop down list select Calibration File Make and click Execute Once these values are recorded quit Data Collection software and shut down the computer 6 Turn off the ABI PRISM 310 Genetic Analyzer Performing the Clear Memory Reset Step Actiom o O Ensure that both the computer and the ABI PRISM 310 Genetic Analyzer are turned off If not shut down the computer first then shut down the instrument 2 Open the instrument doors 3 Press and hold the Gel Pump and Tray buttons down with your right hand Page 1 of 9 Clear Memory Reset Module lied Biosystems Step Action Keeping both buttons depressed reach to the left rear of the instrument and turn on the ABI PRISM 310 Genetic Analyzer After turning the instrument on release the Gel Pump and Tray buttons NOTE If it is performed properly the three status lights will be lit indicating the firmware has been cleared Start the computer If Data Collection software does not launch automatically manually launch it A dialog box will appear i
3. Applied Biosystems To navigate within this document use the 4a gp Or g keys ABI PRISM 310 Genetic Analyzer Clear Memory Reset Procedure BEFORE PERFORMING ANY TROUBLESHOOTING WORK ON YOUR ABI PRISM 310 GENETIC ANALYZER PLEASE READ THE INSTRUMENT USER S MANUAL FOR SAFETY AND WARRANTY INFORMATION AND FURTHER DETAILS ON USE OF THE SYSTEM NOTE Text in this fashion indicates a link to a picture or another section of this another document Please contact AB Technical Support if you have any questions regarding this procedure About the Clear Memory Reset The Clear Memory Reset is the most comprehensive reset that can be performed on the ABI PRISM 310 Genetic Analyzer This reset deletes the firmware and calibration data stored on the instrument Firmware is subsequently reloaded and calibration data must be manually entered Situations in which the Clear Memory Reset may be helpful When the instrument is behaving unpredictably When the instrument and computer are not communicating properly When an instrument subsystem laser heating optics EP or autosampler is suspect When data quality is compromised Before You Begin Since the Clear Memory Reset deletes the calibration data you must record the current calibration values Typically these values will be written on a Calibration sticker on the inside of the instrument s left door If the Calibration sticker or the values are not present follow the procedure below
4. alue entered Select Syringe Home and click Execute This will bring the syringe pump up to its homed position NOTE After homing the syringe pump use the Syringe Down command to bring the syringe pump back on top of the syringe plunger Select Autosampler Home X Y and click Execute Select Autosampler Home Z and click Execute Autosampler Home Z and click Execute Page 2 of 9 Clear Memory Reset Module Applied Biosystems From the Instrument menu select and perform the Autosampler Calibration For a detailed tutorial refer to the document entitled Autosampler Calibration at http home appliedbiosystems com support apptech NOTE After calibrating return the capillary into the water or buffer position 14 Wait 10 minutes to perform a run This allows the temperature circuit to calibrate 45 You have completed the Clear Memory Reset Procedure Page 3 of 9 Clear Memory Reset Module Alert from Instrument Autosampler Needs Calibration Alert MMSN Alert from Instrument A Autosampler Needs Calibration Alert from Instrument CCD Needs Calibration E Alert MMN HBH Alert from Instrument CCO Needs Calibration Use Manual Control to Set H i Y Pixel Positions to Calibrated Values Alert from Instrument Syringe Needs Calibration Alert from Instrument Syringe Needs Calibration Use Manual Control to Set Syringe Mak Travel amp Syringe Pump Force
5. ndicating firmware is being reloaded NOTE If firmware does not automatically load then two problems may exist e No communication between instrument and computer if this occurs press the Reset button once on the back of the instrument For a detailed tutorial to resolve communication issues please refer to the document entitled Resolving Communication Problems at http home appliedbiosystems com support apptech Preferences are corrupted or set improperly For a detailed tutorial regarding setting preferences please refer to the Preferences document appropriate to your operating system at http home appliedbiosystems com support apptech After the firmware has reloaded open Manual Control This will generate a series of alerts e Alert from Instrument Autosampler Needs Calibration e Alert from Instrument CCD Needs Calibration e Alert from Instrument Syringe Needs Calibration 12 Select Autosampler Home Z and click Execute Z O Z 0 These messages are normal Click OK to clear the messages If you performed a Calibration File Make earlier then select Calibration File Send from the Function drop down list and click Execute If you did not perform a Calibration File Make or if you are unsure then select and enter the calibration values for e CCD Pixel Position X horizontal e CCD Pixel Position Y vertical e Syringe Max Travel e Syringe Gel Pump Force NOTE Make sure to click Execute for each v
6. r NOTE After executing the SYRINGE HOME command the syringe pump must be brought back down on top of the plunger Ensure that the pump is close to but not compressing the syringe plunger If the syringe pump is not returned to this position prior to a run the instrument will generate a Leak Detected error po A Gel Pump Force Manual Control SS Function Yal ue Range Syringe Purnp Force 20 to 700 Module none NOTE The number here is for reference only Your instrument may be different Syringe Max Travel C Manual Control 2S Function Yal ue Range Syringe Max Trawel 7 220 to TOO steps Module or T SA sef i eese NOTE The number here is for reference only Your instrument may be different Page 8 of 9 Clear Memory Reset Module Applied Biosystems Three Status Lights For Research Use Only Not for use in diagnostic procedures Notice to Purchaser License Disclaimer Purchase of this software product alone does not imply any license under any process instrument or other apparatus system composition reagent or kit rights under patent claims owned or otherwise controlled by Applera Corporation either expressly or by estoppel Applied Biosystems AB PRISM and GeneScan are registered trademarks and AB Design and Applera are trademarks of Applera Corporation or its subsidiaries in the US and or certain other countries All other trademarks are the sole property of their
7. respective owners Page 9 of 9 Clear Memory Reset Module
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