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Agilent 1260 Infinity Isocratic Pump and Quaternary Pump

1. Solvent cabinet Co ees Pump Q D ope 2 900 Tsss O00 Local user interface Sis E Autosampler a oe LI Thermostatted column compartment a Detector wA CI Figure 10 Recommended Stack Configuration Front View 48 Agilent 1260 IsoPump QuatPump User Manual Installing the Pump 3 AC power CAN bus cable to local user interface Remote cable WU CAN bus cable LAN to control software location depends on detector
2. LS SS SESS a I c A C c Ke SC DC DC lt C E n c Cc n D A L L N Oe lt Jo 6 s paeng IO Lee L as oe I a Remote RS 232 CAN CAN c Config 2 one Agilent Technologies Q CEs c mson wrote ees AME ot fama C C L ie gt Product No G1341B ee lz sl lsle fe 3 c INO van a o aren a c c Serial No DE9xxx110 mwi c c c FUT en ca A moc ER A Zii A C a Figure 6 Rear view of the pump 20 Agilent 1260 IsoPump QuatPump User Manual Interfaces Table 3 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD optional LAN on board RS 232 Analog Introduction 1 The Agilent 1200 Infinity Series modules provide the following interfaces APG Special Remote Pumps G1310B Iso Pump G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G4220A B Bin Pump G1361A Prep Pump Samplers G1329B ALS G2260A Prep ALS G1364B FC PS G1364C FC AS G1364D FC uS G1367E HiP ALS G1377A HiP micro ALS G2258A DL ALS G4226A ALS Detectors G1314B VWD VL G1314C VWD VL G1314E F VWD Yes No Yes Ye
3. Analog detector signal 1 or 2 outputs per detector Figure 11 Recommended Stack Configuration Rear View Agilent 1260 IsoPump QuatPump User Manual 49 3 Installing the Pump Installing the Pump Parts required Preparations p n Description 1 Pump 1 Data System 1 G4208A Instant Pilot 1 Power cord For other cables see text below and Cable Overview on page 166 Locate bench space Provide power connections Unpack the module Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate
4. 79 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Tests and Calibrations System Pressure Test The System Pressure Test is a quick test designed to determine the pressure tightness of the system After exchanging flow path components e g pump seals or injection seal use this test to verify the system is pressure tight 80 Agilent 1260 lsoPump QuatPump User Manual Troubleshooting and Diagnostics 6 Leak Rate Test The Leak Rate Test is a diagnostic test designed to determine the pressure tightness of the pump When a problem with the pump is suspected use this test to help troubleshoot the pump and its pumping performance Agilent 1260 IlsoPump QuatPump User Manual 81
5. The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369A B LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a connected PC with the appropriate control software If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for e 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Agilent 1260 IlsoPump QuatPump User Manual 23 1 24 Introduction Table4 RS 232C Connection Table
6. lt 2 amplitude typically lt 1 3 or lt 3 bar at 1 mL min isopropanol at all pressures gt 10 bar 147 psi User selectable based on mobile phase compressibility 1 0 12 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel Agilent control software For pressure monitoring 1 33 mV bar one output Controller area network CAN RS 232C APG remote ready start stop and shut down signals LAN optional Agilent 1260 lsoPump QuatPump User Manual Site Requirements and Specifications 2 Table 16 Performance Specification Agilent 1260 Infinity Quaternary Pump G1311B Type Hydraulic system Setable flow range Flow range Flow precision Flow accuracy Pressure Pressure pulsation Compressibility compensation Recommended pH range Gradient formation Delay volume Composition range Composition precision Control and data evaluation Analog output Communications Specification Dual piston in series pump with proprietary servo controlled variable stroke drive floating pistons 0 001 10 mL min in 0 001 mL min increments 0 2 10 0 mL min lt 0 07 RSD or lt 0 02 min SD whatever is greater based on retention time at constant room temperature 1 or 10 pL min whatever is greater pumping degassed H 0 at 10 MPa Operating range 0 60 MPa 0 600 bar 0 8700 psi up to 5 mL min Operating range 0 20 MPa 0 200 bar 0
7. 6 Troubleshooting and Diagnostics Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status indicator i YE Agilan Teshwa ogios 1260 Dining co J g Power supply indicator Figure 15 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 82 Agilent 1260 IsoPump QuatPump User Manual Troubleshooting and Diagnostics 6 Module Status Indicator The module status indicator indicates one of six possible module conditions e When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis e A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running e An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the
8. Switch off pump at the main power switch Remove the front cover Use a solvent shutoff valve or lift up solvent filters for avoiding leakages The life time of the multi channel gradient valve can be increased by regularly flushing the valve especially when using buffers If using buffers flush all channels of the valve with water to prevent precipitation of the buffer otherwise salt crystals could drop into an unused channel and form plugs that may cause leaks of that channel Such leaks will interfere with the general performance of the valve When using buffers in combination with organic solvents in the Agilent 1260 Infinity Quaternary Pump it is recommended to connect the aequous solutions buffers to one of the bottom ports and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the buffer channel e g A buffer B organic solvent Agilent 1260 lsoPump QuatPump User Manual Maintenance 9 Exchanging the Multi Channel Gradient Valve MCGV 1 Disconnect the connecting tube waste tube and the solvent tubes from the MCGV unclip them from the tube clips and place them into the solvent cabinet to avoid flow by hydrostatic pressure Solvent tubes Waste tube 2 Press the lower sides of the cover to unclip it Remove the cover 3 Disconnect the MCGV cable unscrew the two screws and remove the valve Screws 4 Pla
9. e e 7 Agilent Technologies Notices Agilent Technologies Inc 2010 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61310 90012 Edition 06 10 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate
10. optional quaternary pump only 3 Syringe Plastic p n 9301 0411 1 Syringe adapter luer barb p n 0100 1681 1 Column Eclipse Plus C18 4 6 x 100 mm 3 5 pm p n 959961 902 optional 1 Column Poroshell 120 EC C18 4 5 x 50 mm 2 7 pm p n 699975 902 optional 1 Agilent 1260 lsoPump QuatPump User Manual Installing the Pump Table 17 Quaternary Pump Checklist Description Quantity 3 Column SB C18 4 6 x 150 mm 5 um p n 883975 902 optional HPLC Starter Kit incl 0 17 mm i d cap p n G4201 68707 optional else HPLC Starter Kit incl 0 12 mmi d cap p n G4202 68707 optional HPLC System Tool Kit p n G4203 68708 optional LAN Communication Interface board p n G1369A or G1369 60001 optional Agilent LC Hardware Documentation DVD p n G4800 64500 optional Agilent Lab Advisor p n G4800 64010 optional else LC HW User Information Utilities DVD p n G4800 64005 Accessory Kit p n G1311 68755 see Accessory Kit G1311 68755 on page 46 1 Isocratic Pump Checklist Isocratic Pump Checklist p n Description G1310 64060 Agilent 1260 Infinity lsocratic Pump G1310B 5065 9981 Solvent cabinet including all plastic parts 9301 1450 Solvent bottle amber G1311 60003 Bottle head assembly 5042 2461 Waste tube purge valve 5 m Power cord 5181 1519 CAN cable Agilent module to module 1 m 03394 60600 Agilent module to 3396A Series integrators Signal cable G1310 90111 Service Manu
11. 10 Both the isocratic pump and quaternary pump are based on a two channel dual plunger in series design which comprises all essential functions that a solvent delivery system has to fulfill Metering of solvent and delivery to the high pressure side are performed by one pump assembly which can generate pressure up to 600 bar In the quaternary pump degassing of the solvents is done in a built in vacuum degasser Solvent compositions are generated on the low pressure side by a high speed proportioning valve MCGV The pump assembly includes a pump head with a passive inlet valve and an outlet valve A damping unit is connected between the two piston chambers A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head An active seal wash optional is available for applications using concentrated buffers as solvents Agilent 1260 lsoPump QuatPump User Manual Hydraulic Path ME Agilent Technologies 1200 laity ie Damper N ke Outlet valve Purge valve Passive inlet valve Pump head Figure 3 Hydraulic Path of the Isocratic Pump Agilent 1260 IsoPump QuatPump User Manual Introduction 1 1 12 Introduction Vacuum chamber Degasser From solvent bottles To sampling unit and column To waste Figure 4 Hydraulic Path
12. 2 Defective leak sensor 3 Leak sensor incorrectly routed being pinched by a metal component Leak Sensor Short Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause 1 Defective flow sensor 2 Leak sensor incorrectly routed being pinched by a metal component Agilent 1260 IsoPump QuatPump User Manual Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative 93 7 94 Error Information Compensation Sensor Open The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Pl
13. Agilent 1260 lsoPump QuatPump User Manual 10 Parts for Maintenance Pump Head Assembly Without Seal Wash 150 Pump Head Assembly with Seal Wash Option 600 bar 152 Outlet Valve 154 Purge Valve Assembly 155 Active Inlet Valve Assembly 156 Accessory Kit G1311 68755 157 Seal Wash Option Kit 158 Solvent Cabinet 159 Bottle Head Assembly 161 Hydraulic Path 162 This chapter provides information on parts for maintenance gy Agilent Technologies 149 10 Parts for Maintenance Pump Head Assembly Without Seal Wash Item p n G1312 60064 1 5067 4695 2 G1312 60062 3 G4220 63015 4 0905 1503 4 0905 1719 5 G1312 25260 6 G1312 60066 G1312 60025 G1312 60020 7 G1312 60067 8 5042 1303 9 G1312 60061 10 0515 2118 G4220 24013 Description Pump Head without Seal Wash Sapphire piston default Piston housing incl spring Support Ring without Seal Wash Piston seal PTFE carbon filled black pack of 2 default or Pump Seal PE yellow pack of 2 Pump housing Passive inlet valve or Active inlet valve body optional without cartridge Cartridge for active inlet valve 600bar Outlet valve complete Screw lock Purge valve assembly Screw M5 60 mm long Backup Ring for Support Ring Complete pump head assembly contains items 1 5 and item 8 For pistons and piston seals see Choosing the Right Pump Seals on page 76 150 Agilent 1260 IsoPump QuatPump User Manual Parts for Maintenance 10
14. Line frequency 50 or 60 Hz 5 Power consumption 180 VA 55 W 188 BTU Maximum Ambient operating 4 55 C 41 131 F temperature Ambient non operating 40 70 C 4 158 F temperature Humidity lt 95 at 25 40 C 77 104 F Non condensing Operating Altitude Up to 2000 m 6562 ft Non operating altitude Up to 4600 m 15091 ft For storing the module Safety standards IEC CSA Installation Category II Pollution Degree 2 For indoor use only UL Agilent 1260 IlsoPump QuatPump User Manual 39 2 Site Requirements and Specifications Performance Specifications 40 Table 15 Performance Specification Agilent 1260 Infinity Isocratic Pump G1310B Type Hydraulic system Setable flow range Flow range Flow precision Flow accuracy Pressure Pressure pulsation Compressibility compensation Recommended pH range Control and data evaluation Analog output Communications Specification Dual piston in series pump with proprietary servo controlled variable stroke drive floating pistons 0 001 10 mL min in 0 001 mL min increments 0 2 10 0 mL min lt 0 07 RSD or lt 0 02 min SD whatever is greater based on retention time at constant room temperature 1 or 10 pL min whatever is greater pumping degassed H 0 at 10 MPa Operating range 0 60 MPa 0 600 bar 0 8700 psi up to 5 mL min Operating range 0 20 MPa 0 200 bar 0 2950 psi up to 10 mL min
15. The quaternary pump is assigned its configuration at the factory If the gradient valve is disconnected and the quaternary pump is rebooted the error message is generated However the pump will function as an isocratic pump in this configuration The error message reappears after each switch on Probable cause Suggested actions 1 Gradient valve disconnected Reconnect the gradient valve Valve Fuse Valve Fuse 0 Channels A and B Valve Fuse 1 Channels C and D The gradient valve in the quaternary pump has drawn excessive current causing the electronic fuse to open Probable cause Suggested actions 1 Defective gradient valve Restart the quaternary pump If the error message appears again exchange the gradient valve 2 Defective connection cable front panel to Please contact your Agilent service main board representative 3 Defective main board Please contact your Agilent service representative Agilent 1260 IlsoPump QuatPump User Manual 99 7 Error Information Inlet Valve Fuse The active inlet valve in the module has drawn excessive current causing the inlet valve electronic fuse to open Probable cause Suggested actions 1 Defective active inlet valve Restart the module If the error message appears again exchange the active inlet valve 2 Defective connection cable front panel to Please contact your Agilent service main board representative 3 Defective main board Please contact your Agilent service represe
16. White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent 1260 IsoPump QuatPump User Manual 171 11 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Pin Universal Pin Agilent Signal Name Active module TTL 1 White Digital ground A O 1 2 B oo Brown Prepare run Low o0 pe DE KEY 3 Gray Start Low o0 e lea 4 Blue Shut down Low BE om 5 Pink Not connected oOo 0 6 Yellow Power on High Ss O 18 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 172 Agilent 1260 lsoPump QuatPump User Manual BCD Cables Identifying Cables 11 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 Violet 2 BCD7 80 4 Blue 3 BCD 6 40 m Yellow 4 BCD 4 10 SS Black 5 BCD 0 1 ay Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100
17. e g the TCC can be controlled through the LAN interface of another module and a CAN connection to that module Figure 9 Configuration switch settings depend on configured mode All modules without on board LAN e default is ALL DIPS DOWN best settings Bootp mode for LAN e for boot test modes DIPS 1 2 must be UP plus required mode Switch settings provide configuration parameters for GPIB address serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN The following tables represent the configuration switch settings for the modules without on board LAN only Agilent 1260 lsoPump QuatPump User Manual 29 1 30 Introduction Table 7 8 bit Configuration Switch without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 rsvo sys rsvo_ Rsvo FC The LAN settings are done on the LAN Interface Card G1369A B Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store
18. notconnected 14 notconnected 15 5V Low Agilent 1260 IlsoPump QuatPump User Manual 173 11 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module 1 1 BCD5 20 2 2 BCD7 80 8e 7 3 3 BCD 6 40 B 4 4 BCD 4 10 5 5 BCDO 1 1e 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 174 Agilent 1260 IlsoPump QuatPump User Manual CAN LAN Cables Identifying Cables 11 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Agilent 1260 IlsoPump QuatPump User Manual 175 11 Identifying Cables External Contact Cable 109 lt 6 15 1 One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent Module Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 a Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 E
19. written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result i
20. 116 site requirements power cords 37 snap fasteners 56 solvent filters cleaning 122 solvent inlet filters 66 67 solvent cabinet 66 67 159 solvent filters checking 121 prevent blocking 68 solvents 187 sonic bath 124 sound emission 186 special interfaces 26 191 Index special settings boot resident 32 forced cold start 32 specification physical 39 status indicator 83 stroke volume 12 stroke length 106 stroke volume 16 synchronization lost 92 system setup and installation optimizing stack configuration 47 T temperature limit exceeded 103 temperature out of range 103 temperature sensor 92 test functions 80 test function introduction 112 timeout 90 tools screwdriver pozidriv 1 140 troubleshooting error messages 80 89 status indicators 80 82 U unpacking the pump 44 V vacuum degasser when to use 72 vacuum degasser 8 vacuum degasser operational hints 72 vacuum degasser 41 57 66 valve failed 100 valve frit 126 valve fuse 99 192 variable reluctance motor 12 variable stroke volume 16 voltage range 39 wW wait timeout 107 waste electrical and electronic equipment 183 wear in procedure 134 WEEE directive 183 weight 39 wrench 1 4 inch 124 126 128 128 130 130 185 135 138 138 wrench 14mm 123 124 126 145 Agilent 1260 lsoPump QuatPump User Manual Index Agilent 1260 IsoPump QuatPump User Manual 193 www agilent com In This Book This manual conta
21. 162 163 missing 44 outlet valve 154 pump head 150 solvent cabinet 159 passive inlet valve 123 pHrange 40 41 physical specifications 39 piston chamber 10 piston 12 66 67 power supply indicator 82 power consideration 36 power consumption 39 power cords 37 power switch 51 pressure above upper limit 97 pressure below lower limit 97 pressure sensor readings 54 pressure pulsation 16 40 41 77 pressure range 76 pressure operating range 40 41 priming witha pump 61 72 with a syringe 72 proportioning valve high speed 10 PTFE frit 126 pump error messages 96 pump head missing 104 pump configuration 99 pump head assembly 150 pump piston 66 67 pump seals 66 purge valve frit 67 p urge valve 66 126 radio interference 185 reassembling the pump head 138 recommended pH range 40 41 remote cable 1 0 removing pump head assembly 128 repairs replacing firmware 147 147 restart without cover 96 RS 232C cable 177 communication settings 30 Agilent 1260 IlsoPump QuatPump User Manual S safety class 180 safety information lithium batteries 184 safety general information 180 standards 39 symbols 182 sapphire piston 12 screwdriver pozidriv 1 140 sealwash 75 when touse 74 sealwash 8 10 74 when touse 75 seal alternative material 76 seals 66 seal wear in 134 selection valve failed 98 serial number information 20 servo restart failed 104 setable flowrange 40 41 shut down 90 simple repairs
22. Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product s Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information Agilent 1260 IlsoPump QuatPump User Manual 183 12 Appendix Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithi
23. Parts required 1 01018 22707 PTFE frit pack of 5 1 1 G1311 60009 Purge valve 5067 4728 Seal cap optional optional Preparations Switch off pump at the main power switch Remove the front cover Use a solvent shutoff valve or lift up solvent filters for avoiding leakages 1 Using a 1 4 inch wrench disconnect the pump outlet 5 Using a pair of tweezers or a toothpick remove the frit capillary at the purge valve 2 Disconnect the waste tube Beware of leaking solvents due to hydrostatic pressure Valve body 3 Using the 14 mm wrench unscrew the purge valve and remove it 4 R th cap from th Ive emove the seal cap from the purge valve PTFE frit Seal cap Before reinstallation always check the gold seal in the seal cap A deformed seal cap should be exchanged 126 Agilent 1260 lsoPump QuatPump User Manual Maintenance 9 6 Place a new frit into the purge valve with the orientation of the frit as shown above 7 Reinstall the cap with the gold seal 8 Insert the purge valve into the pump head and locate the pump outlet capillary and the waste tube Outlet capillary Purge valve Waste tube 9 Tighten the purge valve and reconnect outlet capillary and waste tubing Agilent 1260 IlsoPump QuatPump User Manual 127 9 Maintenance Removing the Pump Head Assembly When Exchanging the seals Exchanging the pistons Exchanging seals of the seal wash op
24. Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 2 RX TX 3 X 3 TX DTR 4 4 DTR cnp 5 Da 5 enD DSR 6 6 DSR RTS 7 7 RTS CTS 8 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 7 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board Agilent 1260 IsoPump QuatPump User Manual Introduction 1 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP
25. Pump The quaternary pump comprises a solvent cabinet a vacuum degasser and a four channel gradient pump The four channel gradient pump comprises a high speed proportioning valve and a pump assembly It provides gradient generation by low pressure mixing A solvent cabinet provides enough space for four one liter bottles An active seal wash optional is available when the quaternary pump is used with concentrated buffer solutions Pump drive Pump head Outlet valve Pump main board Purge valve Passive inlet valve Degasser Figure 1 Overview of the Quaternary Pump 8 Agilent 1260 IlsoPump QuatPump User Manual Introduction 1 Introduction to the Pump Introduction to the Isocratic Pump The isocratic pump has the same operating principle as the quaternary pump but has only one solvent channel that means the composition cannot be changed during a method because there is no multi channel gradient valve MCGV The isocratic pump does not include a degasser An upgrade product Isocratic to Quaternary Pump Upgrade Kit p n G4207 60000 is available for upgrading the isocratic pump to a quaternary pump including a degasser Power supply Pump drive Rass p Main board Pump housing Damper Fan Outlet valve Purge valve Passive inlet valve Figure 2 Overview of the Isocratic Pump Agilent 1260 IlsoPump QuatPump User Manual 9 1 Introduction Overview of the Hydraulic Path
26. QuatPump User Manual ee 2 Site Requirements and Specifications 6 e s e i Site Requirements 36 Physical Specifications 39 Performance Specifications 40 This chapter provides information on environmental requirements physical and performance specifications Agg Agilent Technologies 35 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 14 on page 39 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only WARNING Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed 36 Agilent 1260 lsoPump QuatPump User
27. Reduce the Algae Problem Always use freshly prepared solvents especially use demineralized water which was filtered through about 0 2 um filters Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle Solvent bottle amber p n 9301 1450 supplied with the instrument for your aqueous mobile phase If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase Agilent 1260 lsoPump QuatPump User Manual Agilent 1260 lsoPump QuatPump User Manual 5 Optimiziming Performance When to Use a Vacuum Degasser 72 Operational Hints for the Multi Channel Gradient Valve MCGV 73 When to use the Seal Wash Option 74 Choosing the Right Pump Seals 76 Optimize the Compressibility Compensation Setting 77 This chapter gives hints on how to optimize the performance or use additional devices ott Agilent Technologies n 5 Optimiziming Performance When to Use a Vacuum Degasser 72 The pump does not necessarily require degassing But for the following conditions the vacuum degasser is recommended e if your detector is used with maximum sensitivity in the low UV wavelength range e if your application requires highest injection precision or e if your application requires highest retention time reproducibility mandatory at flow rates below 0 5 mL min Operational Hints for the Vacuum Degasser If you ar
28. Regular Priming 61 Changing Solvents 62 This chapter gives information about the preferred stack setup for your system and the installation of your module ott Agilent Technologies 43 3 Installing the Pump Unpacking the Pump CAUTION If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Delivery Checklist 44 Ensure all parts and materials have been delivered with the pump The delivery checklist is shown in Isocratic Pump Checklist on page 45 and in Table 17 on page 44 To aid in parts identification please refer to chapter Parts and Materials for Maintenance Please report missing or damaged parts to your local Agilent Technologies sales and service office Table 17 Quaternary Pump Checklist Description Quantity Solvent cabinet including all plastic parts p n 5065 9981 1 Solvent bottle amber p n 9301 1450 1 Solvent bottle transparent p n 9301 1420
29. Test failed The test will fail if the sum of all leaks in the system pump autosampler or column compartment and connections exceeds the test limit After isolating and fixing the cause of the leak repeat the System Pressure Test to confirm the system is pressure tight Probable cause Suggested actions 1 Purge valve open Close the purge valve 2 Loose or leaky fittings Tighten the fitting or exchange the capillary 3 Pump Damaged pump seals or pistons Run the Leak Rate Test to confirm the leak 4 Loose purge valve Tighten the purge valve nut 14 mm wrench 5 Autosampler Loose or leaky fitting Tighten or exchange the fitting or capillary 6 Autosampler Rotor seal injection valve Exchange the rotor seal 7 Autosampler Damaged metering seal or Exchange the metering seal Check the piston piston for scratches Exchange the piston if required 8 Autosampler Needle seat Exchange the needle seat 9 Column compartment Loose or leaky fitting Tighten or exchange the fitting or capillary 10 Column compartment Rotor seal column Exchange the rotor seal switching valve Agilent 1260 IlsoPump QuatPump User Manual 113 8 Test Functions and Calibration Leak Rate Test Leak Rate Test Description The Leak Rate Test is a built in troubleshooting test designed to demonstrate the leak tightness of the pump For running the Leak Rate Test and evaluating test results please refer to the online help of LabAdvisor 114 Ag
30. as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The amount of substances should be reduced to the minimal volume required for the analysis gt Do not operate the instrument in an explosive atmosphere 1 Remove the front cover by pressing the snap fasteners on both sides Figure 13 Removing the Front Corver 56 Agilent 1260 IsoPump QuatPump User Manual Installing the Pump 3 Place the solvent cabinet on top of the quaternary pump Put the bottle head assemblies into solvent reservoirs containing your mobile phase and place the bottle in the solvent cabinet Connect the inlet tubes from the bottle head assemblies to the inlet connectors A to D typically the left connection of the channel of the vacuum degasser Fix the tubes in the tube clips of the pump Connect the solvent tubes to the outlet connectors typically right connection of the channel of the vacuum degasser Using a piece of sanding paper connect the waste tubing to the purge valve and place it into your waste system If the pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system Connect the pump outlet capillary pump to injection device to the outlet of the purge valve Agilent 1260 IlsoPump QuatPump User Manual 57 3 Installing the Pump Flow Conne
31. droplets in parts of the system Description 1 Purging solvent s see Table 18 on page 63 Remove the column and replace it by a ZDV fitting Prepare bottles with appropriate intermediate solvents see Table 18 on page 63 1 Ifthe channel is not filled with a buffer proceed to step 4 Place the solvent intake filter into a bottle of water Flush the channel at a flow rate suitable for the installed tubing typically 3 5 mL min for 10 min 4 Modify the flow path of your system as required for your application For delay volume optimization see the Rapid Resolution System manual Buffer salt of aqueous buffers may precipitate in residual isopropanol Capillaries and filter may be cloged by precipitating salt gt Don t perform steps 5 to 7 for channels run with aqueous buffer as solvent 62 Replace the solvent bottle by a bottle of isopropanol Flush the channel at a flow rate suitable for the installed tubing typically 3 5 mL min for 5 min Swap the bottle of isopropanol with a bottle of solvent for your application Repeat steps 1 to 7 for the other channel s of the pump Install the desired column set the required composition and flow rate for your application and equilibrate the system for approx 10 minutes prior to starting a run Agilent 1260 lsoPump QuatPump User Manual Installing the Pump 3 Table 18 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an instal
32. module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis A red blinking modules with on board LAN or yellow blinking modules without on board LAN indicator indicates that the module is in resident mode e g during update of main firmware A fast red blinking modules with on board LAN or fast yellow blinking modules without on board LAN indicator indicates that the module is in boot loader mode e g during update of main firmware In such a case try to re boot the module or try a cold start Agilent 1260 IlsoPump QuatPump User Manual 83 6 Troubleshooting and Diagnostics User Interfaces 84 Depending on the user interface the available tests vary Some descriptions are only available in the service manual Table 20 Test functions available vs user interface Test Instant Pilot G4208A Agilent Lab Advisor System Pressure Test Yes Yes Leak Rate Test No Yes Agilent 1260 lsoPump QuatPump User Manual Troubleshooting and Diagnostics 6 Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagn
33. of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false e fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Table5 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk Agilent 1260 IlsoPump QuatPump User Manual 25 1 26 Introduction Table5 Remote Signal Distribution Pin Signal Description 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example sto
34. of the Quaternary Pump How Does the Pump Work In the quaternary pump the liquid runs from the solvent reservoir through the degasser to the MCGV and from there to the inlet valve For the isocratic pump the solvent bottle is directly connected to the inlet valve The pump assembly comprises two substantially identical piston chamber units Both piston chamber units comprise a ball screw drive and a pump head with one sapphire piston for reciprocating movement in it A servo controlled variable reluctance motor drives the two ball screw drives in opposite directions The gears for the ball screw drives have different circumferences ratio 2 1 allowing the first piston to move at twice the speed of the second piston The solvent enters the pump head close to the bottom Agilent 1260 IsoPump QuatPump User Manual Introduction 1 Overview of the Hydraulic Path limit and leaves it at its top The outer diameter of the piston is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap inbetween The first piston has a stroke volume in the range of 20 100 uL depending on the flow rate The microprocessor controls all flow rates in a range of 1 uL min 10 mL min The inlet of the first pumping unit is connected to the passive inlet valve The outlet of the first piston chamber unit is connected through the outlet valve and the damping unit to the inlet of the second piston chamber unit The outlet o
35. off 7 Purge the quaternary pump see Initial Priming on page 59 5 Connect all capillaries solvent tubes and waste tubing see Flow Setting the 8 bit Configuration Switch without On Board LAN on page 29 The power switch stays pressed in and a green indicator lamp in the power switch is on when the module is turned on When the line power switch stands out and the green light The pump was shipped with default configuration settings To change these settings see Agilent 1260 IsoPump QuatPump User Manual Installing the Pump 3 Connecting Modules and Control Software WARNING Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Connecting Modules 1 Place the individual modules in a stack configuration as shown in Figure 10 on page 48 2 Ensure the power switches on the front of the modules are OFF switches stand out 3 Plug a CAN cable into the CAN connector at the rear of the respective module except vacuum degasser 4 Connect the CAN cable to the CAN connector of the next module see Figure 11 on page 49 5 Press in the power switches to turn on the modules Agilent 1260 IlsoPump QuatPump User Manual 53 3 54 Installing the Pump Connecti
36. the values in the non volatile memory Table 8 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Agilent 1260 lsoPump QuatPump User Manual Introduction 1 Table9 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 10 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 11 Parity Settings without on board LAN Switches Parity 7 8 0 0 No Parity 1 0 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity Agilent 1260 IlsoPump QuatPump User Manual 31 1 Introduction Special Settings The special settings are required for specific actions normally in a service case The tables include both settings for modules with on board LAN and without on board LAN They are identified as LAN and no LAN Boot Resident Firmware update procedures may require this mode in case of
37. to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 118 Agilent 1260 IlsoPump QuatPump User Manual Overview of Maintenance Maintenance The following pages describe maintenance simple repairs of the pump that can be carried out without opening the main cover Table 22 Simple Repair Procedures Procedure Typical Frequency Notes Checking and Cleaning the Solvent Filter on page 121 Exchanging the Passive Inlet Valve PIV on page 123 Exchanging the Outlet Valve on page 124 Exchanging the Purge Valve Frit or the Purge Valve on page 126 Exchanging the Purge Valve Frit or the Purge Valve on page 126 Maintenance of a Pump Head Without Seal Wash Option on page 130 Exchanging pistons see Maintenance of a Pump Head Without Seal Wash Option on page 130 Exchanging the Optional Interface Board on page 143 If solvent filter is blocked If internally leaking If internally leaking If internally leaking If the frit shows indication of contamination or blockage If pump performance indicates seal wear If scratched If defective Gradient performance problems intermittent pressure fluctuations Pressure ripple unstable run Leak Rate Test for verification Pressure ripple unstable run Leak Rate Test for verification Solvent drip
38. use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Bench Space The module dimensions and weight see Table 14 on page 39 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench should carry an Agilent system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 38 Agilent 1260 IsoPump QuatPump User Manual Site Requirements and Specifications 2 Physical Specifications Table 14 Physical Specifications Type Specification Comments Weight 11 kg 25 Ibs Dimensions height x 180 x 345 x 435 mm 7 0 x 13 5 x 17 inches width x depth Line voltage 100 240 VAC 10 Wide ranging capability
39. windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the flow path this problem is less common Symptoms Observed with the Agilent 1260 Infinity HPLC In contrast to the HP 1090 and HP 1050 Series HPLC systems which use helium degassing algae have a better chance to grow in systems such as the Agilent 1260 Infinity where helium is not used for degassing most algae need oxygen and light for growth The presence of algae in the Agilent 1260 Infinity can cause the following to occur e PTFE frits PTFE frit pack of 5 p n 01018 22707 purge valve assembly and column filter blockage causing increased system pressure Algae appear as white or yellowish white deposits on filters Typically black particles from the normal wear of the piston seals do not cause the PTFE Agilent 1260 IlsoPump QuatPump User Manual 69 4 70 Using the Pump frits to block over short term usage Please refer to the section Exchanging the Purge Valve Frit or the Purge Valve on page 126 in this manual Short lifetime of solvent filters bottle head assembly A blocked solvent filter in the bottle especially when only partly blocked is more difficult to identify and may show up as gradient performance problems intermittent pressure fluctuations etc Algae growth may also be the possible source for failures of the ball valves and other components in the flow path How to Prevent and or
40. 0 IlsoPump QuatPump User Manual Appendix 12 Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please ob
41. 2 cables p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m Agilent 1260 IsoPump QuatPump User Manual 167 11 Identifying Cables Analog Cables 168 One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog E57 Agilent 1260 IsoPump QuatPump User Manual Agilent Module to BNC Connector Identifying Cables 11 p n 8120 1840 Pin BNC Pin Agilent Signal Name module ST dl Shield Shield Analog a EHO D Center Center Analog Agilent Module to General Purpose p n 01046 60105 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Black Analog 3 Red Analog E xg Agilent 1260 IlsoPump QuatPump User Manual 169 11 Identifying Cables Remote Cables 170 O O JNE One end of these cables provides a Agilent Technologies APG A
42. 2950 psi up to 10 mL min lt 2 amplitude typically lt 1 3 or lt 3 bar at 1 mL min isopropanol at all pressures gt 10 bar 147 psi User selectable based on mobile phase compressibility 1 0 12 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel Low pressure quaternary mixing gradient capability using proprietary high speed proportioning valve 600 800 uL dependent on back pressure 0 95 or 5 100 user selectable lt 0 2 RSD or lt 0 04 min SD whatever is greater at 0 2 and 1 mL min Agilent control software For pressure monitoring 1 33 mV bar one output Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN optional For use with flow rates below 500 pL min a vacuum degasser is required Agilent 1260 IlsoPump QuatPump User Manual 41 2 42 Site Requirements and Specifications Performance Specifications Agilent 1260 lsoPump QuatPump User Manual Agilent 1260 lsoPump QuatPump User Manual ece 3 Installing the Pump Unpacking the Pump 44 Delivery Checklist 44 Accessory Kit G1311 68755 46 Optimizing the Stack Configuration 47 One Stack Configuration 47 Installing the Pump 50 Connecting Modules and Control Software 53 Connecting Modules 53 Connecting a Vacuum Degasser 54 Connecting Control Software and or G4208 A Instant Pilot 55 Flow Connections of the Pump 56 Priming the System 59 Initial Priming 59
43. 3 Defective pump drive assembly Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative Agilent 1260 IsoPump QuatPump User Manual 103 7 104 Error Information Servo Restart Failed The pump motor in the module was unable to move into the correct position for restarting When the module is switched on the first step is to switch on the C phase of the variable reluctance motor The rotor should move to one of the C positions The C position is required for the servo to be able to take control of the phase sequencing with the commutator If the rotor is unable to move or if the C position cannot be reached the error message is generated Probable cause Suggested actions 1 Disconnected or defective cable Please contact your Agilent service representative 2 Blocked passive inlet valve Exchange the inlet valve 3 Mechanical blockage of the module Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly 4 Defective pump drive assembly Please contact your Agilent service representative 5 Defective main board Please contact your Agilent service representative Pump Head Missing The pump head end stop in the pump was not found When the pump restarts the metering drive moves forward to the mechanical end stop Normally the end stop is reached within 20 seconds indicated by an incre
44. 39 dual piston in series design 10 E electrical connections descriptions of 19 electronic waste 183 electrostatic discharge ESD 143 EMF early maintenance feedback 17 encoder missing 102 error messages fan failed 95 189 Index pump error 96 error messages compensation sensor open 94 compensation sensor short 94 encoder missing 102 ignition without cover 95 95 index adjustment 105 index limit 105 index missing 106 initialization failed 107 inlet valve fuse 100 inlet valve missing 102 leak sensor open 93 leak sensor short 93 leak 92 missing pressure reading 98 motor drive power 101 pressure above upper limit 97 pressure below lower limit 97 pump configuration 99 pump head missing 104 remote timeout 91 selection valve failed 98 servo restart failed 104 shut down 90 stroke length 106 synchronization lost 92 temperature limit exceeded 103 temperature out of range 103 timeout 90 valve failed 100 valve fuse 99 error message wait timeout 107 error solvent zero counter 96 exchanging active inlet valve 145 active inlet valve cartridge 145 inlet valve 119 190 multi channel gradient valve MCGV 140 outlet valve 119 124 passive inlet valve 123 pistons 119 pump seals 119 purge valve frit 119 126 purge valve 119 126 external contact cable 1 6 F fan failed 95 firmware updates 147 147 upgade downgrade 147 upgrade downgrade 147 flow accuracy 40 41 flow connections 56 flow precis
45. 56 Accessory Kit G1311 68755 157 Seal Wash Option Kit 158 Solvent Cabinet 159 Bottle Head Assembly 161 Hydraulic Path 162 1 Identifying Cables 165 Cable Overview 166 Analog Cables 168 Remote Cables 170 BCD Cables 173 CAN LAN Cables 175 External Contact Cable 176 Agilent Module to PC 177 Agilent 1200 Module to Printer 178 12 Appendix 179 General Safety Information 180 The Waste Electrical and Electronic Equipment Directive 183 Batteries Information 184 Radio Interference 185 Sound Emission 186 Solvent Information 187 Agilent Technologies on Internet 188 Agilent 1260 IsoPump QuatPump User Manual Contents 6 Agilent 1260 IlsoPump QuatPump User Manual Agilent 1260 IsoPump QuatPump User Manual 1 Introduction Introduction to the Pump 8 Overview of the Hydraulic Path 10 Hydraulic Path 11 How Does the Pump Work 12 How Does Compressibility Compensation Work 15 How Does Variable Stroke Volume Work 16 Early Maintenance Feetback 17 Instrument Layout 18 Electrical Connections 19 Serial Number Information ALL 20 Rear View of the Module 20 Interfaces 21 Overview Interfaces 23 Setting the 8 bit Configuration Switch 27 Communication Settings for RS 232C 30 Special Settings 32 This chapter gives an introduction to the module instrument overview and internal connectors ee Agilent Technologies 1 Introduction Introduction to the Pump Introduction to the Pump Introduction to the Quaternary
46. Figure 19 Pump Head Assembly without seal wash option Agilent 1260 IlsoPump QuatPump User Manual 151 10 Parts for Maintenance Pump Head Assembly with Seal Wash Option 600 bar Item p n Description G1312 60065 Pump Head with Seal Wash 5067 4695 Sapphire piston default G1312 60062 Piston housing incl spring G4220 63010 Support Ring Seal Wash Aea wo N 0905 1175 Wash seal PTFE carbon filled or 4 0905 1718 Wash Seal PE 0890 1764 Tubing wash option 5 01018 07102 Gasket Seal wash 6 G4220 26210 Seal Holder G4220 24013 Backup Ring for Support Ring 7 0905 1503 Piston seal PTFE carbon filled black pack of 2 default or 7 0905 1719 Pump Seal PE yellow pack of 2 8 G1312 25260 Pump housing 9 G1312 60066 Passive inlet valve or 9 G1312 60025 Active inlet valve body optional without cartridge G1312 60020 Cartridge for active inlet valve 600bar 10 G1312 60067 Outlet valve complete 11 5042 1303 Screw lock 12 G1312 60061 Purge valve assembly 152 Agilent 1260 lsoPump QuatPump User Manual Parts for Maintenance 10 Item p n Description 13 0515 2118 Screw M5 60 mm long 14 G1311 60161 Seal wash pump assembly Figure 20 Pump Head with Seal Wash Option Agilent 1260 IsoPump QuatPump User Manual 153 10 Parts for Maintenance Outlet Valve Outlet Valve p n Description G1312 60067 Outlet valve complete Outlet Valve Figure 21 154 Agilent 1260 IlsoPump QuatPump User Manual Parts for Maint
47. IlsoPump QuatPump User Manual Solvent Cabinet Item p n 1 5065 9981 2 5043 0207 4 5042 8567 5 9301 1420 6 9301 1450 7 G1311 60003 Parts for Maintenance 10 Solvent Cabinet Description Solvent cabinet including all plastic parts Name plate 1260 Leak pan Solvent bottle transparent Solvent bottle amber Bottle head assembly Agilent 1260 IlsoPump QuatPump User Manual 159 10 Parts for Maintenance Solvent Cabinet 5 6 Figure 23 Solvent Cabinet Parts 160 Agilent 1260 lsoPump QuatPump User Manual Bottle Head Assembly Item p n G1311 60003 5063 6598 5063 6599 Aa wo N 5062 2483 ol 5062 8517 5041 2168 Parts for Maintenance 10 Description Bottle head assembly Ferrules with lock ring 10x Tube screw 10x Wire marker Solvent tubing 5 m Inlet filter adapter pack of 4 Solvent inlet filter 20 pm Figure 24 Bottle Head Assembly Parts Agilent 1260 IsoPump QuatPump User Manual 161 10 Parts for Maintenance Hydraulic Path Item p n 1 G1311 67301 G1311 60003 2 G1311 67300 3 G1312 67305 G1329 87300 4 5062 2461 0100 1847 Description Capillary piston 1 to damper Bottle head assembly Capillary damper to piston 2 Outlet capillary pump to injector device Outlet capillary pump to thermostattable autosampler Waste tube 5 m reorder pack Adapter AIV to solvent inlet tubes A Agila Vochnatogin
48. Manual CAUTION Site Requirements and Specifications 2 Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1260 IsoPump QuatPump User Manual 37 2 Site Requirements and Specifications WARNING Unintended
49. XT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 176 Agilent 1260 lsoPump QuatPump User Manual Identifying Cables 11 Agilent Module to PC p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m Agilent 1260 IlsoPump QuatPump User Manual 177 11 Identifying Cables Agilent 1200 Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control Module 178 Agilent 1260 lsoPump QuatPump User Manual Agilent 1260 lsoPump QuatPump User Manual 12 Appendix General Safety Information 180 The Waste Electrical and Electronic Equipment Directive 183 Batteries Information 184 Radio Interference 185 Sound Emission 186 Solvent Information 187 Agilent Technologies on Internet 188 This chapter provides addition information on safety legal and web ee Agilent Technologies 179 12 Appendix General Safety Information The follow
50. ack when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Agilent 1260 IlsoPump QuatPump User Manual 17 1 Introduction Instrument Layout The industrial design of the module incorporates several
51. actions 50 1 Place the module on the bench in a horizontal position Agilent 1260 lsoPump QuatPump User Manual Installing the Pump 3 Installing the Pump 2 Ensure the power switch on the front of the module is OFF switch stands out Status lamp xe Agilent Techwalogies 12660 Itinity Name plate Power switch Serial number Figure 12 Front of Pump 3 Connect the power cable to the power connector at the rear of the module Agilent 1260 IlsoPump QuatPump User Manual 51 3 Configuration switch Installing the Pump 4 Connect the required interface cables to the quaternary pump see Connecting Modules and Control Software on page 53 Slot for interface board Analog pressure APG remote RS 232C CAN Power 52 E Agilent Technologies Product No G1344B OO ie Toek rabea Made in Germany Serial No DE900110 Config mogo D Connections of the Pump on page 56 6 Press the power switch to turn on the module is off the module is turned
52. al G1310 68755 Accessory Kit Agilent 1260 lsoPump QuatPump User Manual 45 3 Installing the Pump Accessory Kit G1311 68755 Accessory Kit G1311 68755 p n Description 5062 2461 Waste tube 5 m reorder pack 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 5181 1519 CAN cable Agilent module to module 1 m 5988 8453EN Capillary fitting starter kit brochure 9222 0519 Bag plastic G1329 87300 Capillary 0 17 mm 900 mm G1311 90107 Algae note 5042 9954 Tubing clip 2x re order 4 pk G1311 60003 Bottle head assembly 46 Agilent 1260 lsoPump QuatPump User Manual Installing the Pump 3 Optimizing the Stack Configuration If your module is part of a complete Agilent 1260 Infinity Liquid Chromatograph you can ensure optimum performance by installing the following configurations These configurations optimize the system flow path ensuring minimum delay volume One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1260 Infinity LC System in the following configuration see Figure 10 on page 48 and Figure 11 on page 49 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required Agilent 1260 IlsoPump QuatPump User Manual 47 3 Installing the Pump
53. alt solution and the B channel of the pump is used for the organic solvent If used in this configuration the salt will fall back into the salt solution and will be dissolved When using the pump in a different configuration e g D salt solution A organic solvent the salt can fall into the port of the organic solvent and may lead to performance problems When using salt solutions and organic solvents it is recommended to connect the salt solution to one of the bottom ports of the MCGV and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the salt solution channel Regular flushing with water of all MCGV channels is recommended to remove all possible salt deposits in the valve ports Agilent 1260 IlsoPump QuatPump User Manual 73 5 Optimiziming Performance When to use the Seal Wash Option CAUTION Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent The seal wash option is strongly recommended when buffer concentrations of 0 1 M or higher will be used for long time periods in the pump The active seal wash upgrade can be ordered as G1398A The seal wash option comprises a support ring secondary seal gasket and seal holder for both piston sides A wash bottle filled with water isopropanol 90 10 is p
54. alth risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used The purge tool of the LabAdvisor or Instrument Utilities can be used for automatically purging the pump If the pump is not able to aspirate the solvent from the bottles a syringe can be used to draw the solvent manually through tubing and degasser Agilent 1260 IlsoPump QuatPump User Manual 59 3 60 Installing the Pump When priming the vacuum degasser with a syringe the solvent is drawn through the degasser tubes very quickly The solvent at the degasser outlet will therefore not be fully degassed Pump for approximately 10 minutes at your desired flow rate before starting an analysis This will allow the vacuum degasser to properly degas the solvent in the degasser tubes oF Ww N Open the purge valve of the pump Set the flow rate to 5 mL min Select channel Al Turn the flow on Observe if the solvent in the tubing of channel A1 is advancing towards the pump If it isn t disconnect the solvent tubing from the solvent selection valve attach a syringe with a syringe adapter and pull the liquid through the degasser Reattach the tubing to the solvent selection valve Pump 30 mL isopropanol to remove residual air bubbles Switch to the next solvent ch
55. alve active inlet valve outlet valve or piston seals 3 Flow changed after starting test 4 Defective pump drive assembly Degasser cannot read signal Suggested actions Ensure that purge valve is closed Ensure pump components are seated correctly If there are still signs of a leak exchange the appropriate seal purge valve active inlet valve outlet valve piston seal Ensure correct operating condition for the special application in use Please contact your Agilent service representative The pump board gets no or wrong pressure signals from the built in degasser Probable cause 1 Degasser board defect missing or not connected to the pump main board 2 Degasser sensor defect or not connected to degasser board Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1260 IlsoPump QuatPump User Manual Error Information 7 Degasser limit not reached This error is thrown if the degasser does not become ready after 8 min i e is higher than 180 mbar Probable cause Suggested actions 1 Liquid in degasser tubing Please contact your Agilent service representative 2 Leak in degasser tubing or chamber Please contact your Agilent service representative 3 Degasser vacuum pump defect Please contact your Agilent service representative Agilent 1260 IsoPump QuatPump User Manual 109 7 Error Information Module Error M
56. am is in place If the foam is removed the fan is switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during operation Reinstall the top foam 2 Foam not activating the sensor Please contact your Agilent service representative 3 Dirty or defective sensor Please contact your Agilent service representative 4 Rear of the module is exposed to strong Ensure that the rear of module is not directly direct sunlight exposed to strong sunlight Agilent 1260 IsoPump QuatPump User Manual 95 7 Error Information Module Error Messages 96 These errors are pump specific Restart Without Cover The module was restarted with the top cover and foam open The sensor on the main board detects when the top foam is in place If the module is restarted with the foam removed the module switches off within 30 s and the error message is generated Probable cause Suggested actions 1 The module started with the top cover and Please contact your Agilent service foam removed representative 2 Rear of the module is exposed to strong Ensure that the rear of module is not directly direct sunlight exposed to strong sunlight Solvent Zero Counter Pump firmware version A 02 32 and higher allow to set solvent bottle fillings in the data system If the volume level in the bottle falls below the specified value the error message appears when the feature is configured accordingly Probab
57. anently block capillaries and valves Therefore always filter solvents through 0 4 um filters e Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid and nitric acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCly O gt gt 2COCl 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF Agilent 1260 IlsoPump QuatPump User Manual 187 12 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download 188 Agilent 1260 IlsoP
58. annel and repeat steps 5 and 6 until all channels have been purged Turn the flow off and close the purge valve Agilent 1260 lsoPump QuatPump User Manual Installing the Pump 3 Regular Priming When When the pumping system has been turned off for a certain time for example overnight air will rediffuse into the solvent channel between the vacuum degasser and the pump Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time Preparations Switch the system on The purge tool of the LabAdvisor or Instrument Utilities can be used for automatically purging the pump 1 Open the purge valve of your pump by turning it counterclockwise and set the flow rate to 5 mL min Flush the vacuum degasser and all tubes with at least 10 mL of solvent Repeat step 1 and 2 for the other channel s of the pump Set the required composition and flow rate for your application and close the purge valve 5 Pump for approximately 10 minutes before starting your application Agilent 1260 IlsoPump QuatPump User Manual 61 3 Installing the Pump Changing Solvents When Parts required Preparations CAUTION When the solvent of a channel is to be replaced by another solvent that is not compatible solvents are immiscible or one solvent contains a buffer it is necessary to follow the procedure below to prevent clogging of the pump by salt precipitation or residual liquid
59. ase in motor current If the end point is not found within 20 seconds the error message is generated Probable cause Suggested actions 1 Pump head not installed correctly screws Install the pump head correctly Ensure nothing not secured or pump head not seated e g capillary is trapped between the pump correctly head and body 2 Broken piston Exchange the piston Agilent 1260 IsoPump QuatPump User Manual Error Information 7 Index Limit The time required by the piston to reach the encoder index position was too short pump During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is reached too fast the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative Index Adjustment The encoder index position in the module is out of adjustment During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the time to reach the index position is too long the error message
60. be is needed 1 Remove the front cover 2 Unplug the active inlet valve cable from the connector Disconnect the solvent inlet tube from the inlet valve be aware that solvent may leak out of the tube due to hydrostatic flow 4 Unscrew the adapter from the active inlet valve Agilent 1260 IsoPump QuatPump User Manual 145 9 Maintenance 5 Using a 14 mm wrench loosen the active inlet valve and remove the valve from the pump head Active inlet valve body oy Valve cartridge Figure 18 Active Inlet Valve Assembly 6 Using a pair of tweezers remove the valve cartridge from the actuator assembly 7 Before inserting the new valve cartridge clean the area in the actuator assembly Flush the cartridge area thoroughly with alcohol 8 Insert a new cartridge into the actuator assembly make sure the valve cartridge is completely inserted into the actuator assembly 9 Insert the new valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight 10 Position the valve so that the solvent inlet tube connection points towards the front 11 Using the 14 mm wrench tighten the nut by turning the valve in its final position not more than a quarter turn 12 Reconnect the adapter at the active inlet valve 13 Reconnect the solvent inlet tube to the adapter Reconnect the active inlet valve cable to the connector in the Z panel 14 Reinstall the front cover 15 Purge the system with 30 mL of solvent in orde
61. ce the new MCGV into position Make sure that channel A of the MCGV is put at the bottom right position Tighten the two screws and connect the cable to its connector Screws Agilent 1260 IlsoPump QuatPump User Manual 141 9 Maintenance Exchanging the Multi Channel Gradient Valve MCGV 5 Install the MCGV cover Reconnect the waste funnel with the waste tube holder in the top cover Insert waste tube in the holder in the leak pan and clip tube to the MCGV cover 6 Reconnect the tube from the inlet valve to the middle position of the MCGV Connect solvent tubes for channels A D from the MCGV to the degasser outlets Solvent tubes Waste tube 142 Agilent 1260 lsoPump QuatPump User Manual Maintenance 9 Exchanging the Optional Interface Board When Parts required CAUTION Board defective p n Description 1 G1351 68701 Interface board BCD with external contacts and BCD outputs Electronic boards are static sensitive and should be handled with care so as not to damage them Touching electronic boards and components can cause electrostatic discharge ESD ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components 1 Switch off the pump at the main power switch unplug
62. ctions of the Pump Bottle head assembly Tubings Solvent cabinet Inlet Outlet MCGV Purge valve Waste tubing Outlet capillary to autosampler 58 9 Prime your system before first use see Initial Priming on page 59 E _ J m fe i QO L S 2 7 j af pe Q oO 2 D A a s nll TE 7 Uu oC Figure 14 Flow Connections of the Quaternary Pump Agilent 1260 IsoPump QuatPump User Manual Installing the Pump 3 Priming the System Initial Priming When Parts required Preparations Before a new degasser or new solvent tubing can be used it is necessary to prime the system Isopropanol IPA is recommended as priming solvent due to its miscibility with nearly all HLPC solvents and its excellent wetting properties Description 1 lsopropanol Connect all modules hydraulically as described in the respective module manuals Fill each solvent bottle with 100 mL isopropanol Switch the system on When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can bear he
63. ctive CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Loose or leaking purge valve inlet valve or Ensure pump components are seated correctly outlet valve If there are still signs of a leak exchange the appropriate seal purge valve inlet valve outlet valve 4 Defective pump seals Exchange the pump seals Agilent 1260 lsoPump QuatPump User Manual Leak Sensor Open Error Information 7 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause 1 Leak sensor not connected to the main board
64. d by a black or yellow surface deposits or by a pressure greater than 10 bar when pumping distilled water at a rate of 5 mL min with an open purge valve When using the quaternary pump at low flow rates for example 0 2 mL min check all 1 16 inch fittings for any signs of leaks Whenever exchanging the pump seals the purge valve frit should be exchanged too When using buffers or other salt solutions flush the system with water before switching it off The seal wash option should be used when salt concentrations of 0 1 M or higher will be used for long time periods Check the pump pistons for scratches when changing the piston seals Scratched pistons will cause micro leaks and will decrease the lifetime of the seal Pressurize the system according to the wear in procedure after changing the piston seals see Maintenance of a Pump Head Without Seal Wash Option on page 130 Consider recommendations given in the solvent information section see Solvent Information on page 187 Agilent 1260 IlsoPump QuatPump User Manual 67 4 Using the Pump Prevent Blocking of Solvent Filters 68 Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performanc
65. drive assembly Please contact your Agilent service representative 106 Agilent 1260 lsoPump QuatPump User Manual Error Information 7 Initialization Failed The module failed to initialize successfully within the maximum time window A maximum time is assigned for the complete pump initialization cycle If the time is exceeded before initialization is complete the error message is generated Probable cause Suggested actions 1 Blocked passive inlet valve Exchange the inlet valve 2 Defective pump drive assembly Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Wait Timeout When running certain tests in the diagnostics mode or other special applications the pump must wait for the pistons to reach a specific position or must wait for a certain pressure or flow to be reached Each action or state must be completed within the timeout period otherwise the error message is generated Possible Reasons for a Wait Timeout Pressure not reached Pump channel A did not reach the delivery phase Pump channel B did not reach the delivery phase Pump channel A did not reach the take in phase Pump channel B did not reach the take in phase Solvent volume not delivered within the specified time Agilent 1260 IsoPump QuatPump User Manual 107 7 108 Error Information Probable cause 1 Purge valve open 2 Leak at fittings purge v
66. e 1260 pump that has the degasser built in A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Agilent 1260 IsoPump QuatPump User Manual Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation 91 7 92 Error Information Synchronization Lost Leak During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defe
67. e of the module Use a sterile if possible amber solvent bottle to slow down algae growth Filter solvents through filters or membranes that remove algae Exchange solvents every two days or refilter If the application permits add 0 0001 0 001M sodium azide to the solvent Place a layer of argon on top of your solvent Avoid exposure of the solvent bottle to direct sunlight Never use the system without solvent filter installed Agilent 1260 lsoPump QuatPump User Manual Using the Pump 4 Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing e Deposits on ball valves inlet or outlet resulting in unstable flow or total failure of the pump e Small pore solvent inlet filters to plug resulting in unstable flow or total failure of the pump Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure e Column filters to plug giving high system pressure e Flow cell
68. e plunger seals Scratched plungers will lead to micro leaks and will decrease the lifetime of the seal After changing plunger seals apply the seal wear in procedure see Maintenance of a Pump Head Without Seal Wash Option on page 130 Agilent 1260 lsoPump QuatPump User Manual Using the Pump 4 Hints for Successful Use of the Quaternary Pump Always place the solvent cabinet with the solvent bottles on top of the quaternary pump or at a higher level When using salt solutions and organic solvents in the Agilent 1260 Infinity Quaternary Pump VL it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the salt solution channel Regular flushing of all MCGV channels with water is recommended to remove all possible salt deposits in the valve ports Before operating the quaternary pump flush the pump and vacuum degasser see Regular Priming on page 61 This is especially recommended if it has been turned off for some time for example overnight and volatile solvent mixtures are used in the channels Prevent blocking of solvent inlet filters Never use the pump without solvent inlet filter Prevent the growth of algae see Prevent Blocking of Solvent Filters on page 68 Regularly check the purge valve frit and column frit A blocked purge valve frit can be identifie
69. e timeout function switched off the module as requested 2 Anot ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Shut Down Suggested actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required An external instrument has generated a shut down signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Agilent 1260 lsoPump QuatPump User Manual Probable cause 3 Shut down in an external instrument with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing Remote Timeout Error Information 7 Suggested actions Check external instruments for a shut down condition Check the vacuum degasser for an error condition Refer to the Service Manual for the degasser or th
70. e using the vacuum degasser for the first time if the vacuum degasser was switched off for any length of time for example overnight or if the vacuum degasser lines are empty you should prime the vacuum degasser before running an analysis The vacuum degasser can be primed either by drawing solvent through the degasser with a syringe or by pumping with the pump Priming the degasser with a syringe is recommended when e vacuum degasser is used for the first time or vacuum tubes are empty or e changing to solvents that are immiscible with the solvent currently in the vacuum tubes Priming the vacuum degasser by using the pump at high flow rate 3 5 mL min is recommended when pump was turned off for a length of time for example during night and volatile solvent mixtures are used or e solvents have been changed For more information see the Agilent 1260 Infinity Standard Degasser User Manual p n G1322 90012 Agilent 1260 lsoPump QuatPump User Manual Optimiziming Performance 5 Operational Hints for the Multi Channel Gradient Valve MCGV In a mixture of salt solutions and organic solvent the salt solution might be well dissolved in the organic solvent without showing precipitations However in the mixing point of the gradient valve at the boundary between the two solvents micro precipitation is possible Gravity forces the salt particles to fall down Normally the A channel of the valve is used for the aqueous s
71. eal wash gasket Agilent 1260 lsoPump QuatPump User Manual Mai ntenance of a Pump Head with Maintenance eal Wash Option 5 Place the support rings on the piston housing pistons not installed and snap the pump head and piston housing together Pump head Support ring Piston housing 6 Insert the pistons and carefully press them into the seal Piston Agilent 1260 IlsoPump QuatPump User Manual 137 9 Maintenance Reinstalling the Pump Head Assembly When When reassembling the pump Tools required 4 mm hexagonal key Parts required Description 79846 65501 Pump head grease 1 Apply a small amount of grease on the back of the 2 Slide the pump head assembly onto the pump drive pistons Balls of spindle drive L d a ak Th gt l Metering drive Pump head AUA 138 Agilent 1260 IsoPump QuatPump User Manual Maintenance 9 Reinstalling the Pump Head Assembly 3 Using a 4 mm hexagonal key tighten the pump head 4 Reconnect all capillaries tubes and if installed the screws stepwise with increasing torque active inlet valve cable to its connector 5 Reinstall the front cover Agilent 1260 IsoPump QuatPump User Manual 139 9 Maintenance Exchanging the Multi Channel Gradient Valve MCGV Tools required Parts required Preparations 140 Screwdriver Pozidriv 1 p n Description 1 G1311 69701 MCGV exchange assembly
72. ease contact your Agilent service representative Compensation Sensor Short The ambient compensation sensor NTC on the main board in the module has failed short circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Agilent 1260 lsoPump QuatPump User Manual Error Information 7 Fan Failed The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 4 Improperly positioned cables or wires Ensure the fan is not mechanically blocked obstructing fan blades Open Cover The top foam has been removed The sensor on the main board detects when the top fo
73. ed with new standard seals at 600 bar 76 Agilent 1260 lsoPump QuatPump User Manual Optimiziming Performance 5 Optimize the Compressibility Compensation Setting The compressibility compensation default setting is 100 x 106 bar for the pump This setting represents an average value Under normal conditions the default setting reduces the pressure pulsation to values below 1 of system pressure that will be sufficient for most applications and for all gradient analyses For applications using sensitive detectors the compressibility settings can be optimized by using the values for the various solvents described in Table 19 on page 78 If the solvent in use is not listed in the compressibility tables when using isocratic mixtures of solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the compressibility settings When using mixtures of solvents it is not possible to calculate the compressibility of the mixture by interpolating the compressibility values of the pure solvents used in that mixture or by applying any other calculation In these cases the following empirical procedure has to be applied to optimize your compressibility setting 1 Start the pump with the required flow rate 2 Before starting the optimization procedure the flow must be stable Check the tightness of the system with the pressure test 3 Your pump must be connected to a data system or Insta
74. enance 10 Purge Valve Assembly Item p n Description 1 G1312 60061 Purge valve assembly 2 01018 22707 PTFE frit pack of 5 3 5067 4728 Seal cap Agilent 1260 IlsoPump QuatPump User Manual 155 10 Parts for Maintenance Active Inlet Valve Assembly Item p n Description 1 G1312 60025 Active inlet valve without cartridge 2 G1312 60020 Cartridge for active inlet valve 600bar Figure 22 Active Inlet Valve Assembly 156 Agilent 1260 lsoPump QuatPump User Manual Parts for Maintenance 10 Accessory Kit G1311 68755 Accessory Kit G1311 68755 p n Description 5062 2461 Waste tube 5 m reorder pack 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 5181 1519 CAN cable Agilent module to module 1 m 5988 8453EN Capillary fitting starter kit brochure 9222 0519 Bag plastic G1329 87300 Capillary 0 17 mm 900 mm G1311 90107 Algae note 5042 9954 Tubing clip 2x re order 4 pk G1311 60003 Bottle head assembly Agilent 1260 IlsoPump QuatPump User Manual 157 10 Parts for Maintenance Seal Wash Option Kit p n Description G1311 60161 Seal wash pump assembly 5042 8507 Seal wash pump cartridge silicone tubing 5062 2465 Support ring seal wash 0905 1175 Secondary seal pre installed in support rings 5062 2484 Gasket seal wash pack of 6 5001 3743 Seal holder 0890 1764 Silicone rubber tubing 1 mm i d 3 m 5063 6589 Standard seals pack of 2 01018 2370 Seals insert tool 158 Agilent 1260
75. essages 110 Agilent 1260 IlsoPump QuatPump User Manual Agilent 1260 IlsoPump QuatPump User Manual 8 Test Functions and Calibration Introduction 112 System Pressure Test 113 System Pressure Test failed 113 Leak Rate Test 114 Leak Rate Test Description 114 This chapter describes the tests for the module egg Agilent Technologies 111 8 Test Functions and Calibration Introduction 112 All tests are described based on the Agilent Lab Advisor Software B 01 03 Other user interfaces may not provide any test or just a few For details on the use of the interface refer to the interface documentation Table 21 Interfaces and available test functions Interface Comment Available Function Agilent Instrument Utilities System Pressure Test Agilent Lab Advisor All tests are available System Pressure Test Leak Rate Test Agilent ChemStation No tests available Agilent Instant Pilot System Pressure Test Agilent 1260 IlsoPump QuatPump User Manual Test Functions and Calibration 8 System Pressure Test Description The System Pressure Test is a quick built in test designed to demonstrate the pressure tightness of the system The test is required if problems with small leaks are suspected or after maintenance of flow path components e g pump seals injection seal to prove pressure tightness up to 600 bar For running the test please refer to the online help of the diagnostic software System Pressure
76. f the purge valve assembly is then connected to the following chromatographic system Damper NJ x Va Pump chamber 2 Purge valve Pump chamber 1 gt Tocolumn Inlet valve To waste From solvent bottle degasser Seal Piston 1 Piston 2 Ball screw drive Gear Motor with encoder Figure 5 Principle of the Pump Agilent 1260 IlsoPump QuatPump User Manual 13 1 14 Introduction When turned on the pump runs through an initialization procedure to determine the upper dead position of the first piston The first piston moves slowly upwards into the mechanical stop of the pump chamber and from there it moves back for a defined distance The controller stores this piston position in memory After this initialization the pump starts operation with the set parameters The passive inlet valve opens and the down moving piston draws solvent into the first pump chamber At the same time the second piston moves upwards delivering to the system After a controller defined stroke length that depends on the flow rate the drive motor is stopped and the passive inlet valve closes The motor direction is reversed and moves the first piston up until it reaches the stored upper limit and at the same time the second piston moves downwards Then the sequence starts again moving the pistons up and down between the two limits During the up movement of the first piston the solvent in the pump cha
77. firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 12 Boot Resident Settings without on board LAN Mode Select SW1 sw2 SW3 SW4 SW5 SW6 SW7 Swe LAN No LAN TEST BOOT 1 1 1 0 0 0 0 0 TEST BOOT 1 1 0 0 1 0 0 0 CAUTION Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start 32 If you use the following switch settings and power the instrument up again a forced cold start has been completed Agilent 1260 lsoPump QuatPump User Manual Introduction 1 Table 13 Forced Cold Start Settings without on board LAN Mode Select SW1 sw2 SW3 sw4 SW5 SW6 SW7 SW8 LAN TEST BOOT 1 1 0 0 0 0 0 1 No LAN TEST BOOT 1 1 0 0 1 0 0 1 Agilent 1260 IlsoPump QuatPump User Manual 33 1 34 Introduction Setting the 8 bit Configuration Switch Agilent 1260 lsoPump QuatPump User Manual Agilent 1260 IsoPump
78. ge Valve Frit or the Purge Valve on page 126 Agilent 1260 IlsoPump QuatPump User Manual 133 9 Maintenance Seal Wear in Procedure Parts required CAUTION Description 0100 1847 Adapter AIV to solvent inlet tubes 5022 2159 Restriction capillary Seal damage gt This procedure is required for Standard seals pack of 2 p n 5063 6589 but it will damage the PE seals pack of 2 p n 0905 1420 134 Place a bottle with 100 mL of isopropanol in the solvent cabinet and place a tubing including bottle head assembly in the bottle If an AIV is installed screw the Adapter AIV to solvent inlet tubes p n 0100 1847 to the AIV and connect the inlet tube from the bottle head directly to it Connect the restriction capillary 5022 2159 to the purge valve Insert its other end into a waste container Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 mL min Close the purge valve set the flow to a rate adequate to achieve a pressure of 350 bar Pump 15 min at this pressure to wear in the seals The pressure can be monitored using your instrument control software or tool Turn OFF the pump slowly open the purge valve to release the pressure from the system disconnect the restriction capillary and reinstall the bottle with the solvent for your application 7 Rinse your system with the solvent used for your next application 8 Replace the purge valve frit see Exchang
79. hether the seals or other module components are defective Agilent 1260 IlsoPump QuatPump User Manual 97 7 Error Information Probable cause Suggested actions 4 Defective damper Please contact your Agilent service representative 5 Defective main board Please contact your Agilent service representative Pressure Signal Missing The pressure signal of the damper is missing The pressure signal of the damper must be within a specific voltage range If the pressure signal is missing the processor detects a voltage of approximately 120mV across the damper connector Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative Missing Pressure Reading The pressure readings read by the pump ADC analog digital converter are missing The ADC reads the pressure signal of from the damper every Ims If the readings are missing for longer than 10 seconds the error message is generated Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 98 Agilent 1260 IsoPump QuatPump User Manual Error Information 7 Pump Configuration At switch on the quaternary pump has recognized a new pump configuration
80. ilent 1260 lsoPump QuatPump User Manual Agilent 1260 lsoPump QuatPump User Manual 9 Maintenance Introduction to Maintenance and Repair 116 Warnings and Cautions 117 Overview of Maintenance 119 Cleaning the Module 120 Checking and Cleaning the Solvent Filter 121 Cleaning the Solvent Filter 122 Exchanging the Passive Inlet Valve PIV 123 Exchanging the Outlet Valve 124 Exchanging the Purge Valve Frit or the Purge Valve 126 Removing the Pump Head Assembly 128 Maintenance of a Pump Head Without Seal Wash Option 130 Seal Wear in Procedure 134 Maintenance of a Pump Head with Seal Wash Option 135 Reinstalling the Pump Head Assembly 138 Exchanging the Multi Channel Gradient Valve MCGV 140 Exchanging the Optional Interface Board 143 Exchanging the Active Inlet Valve AIV or its Cartridge 145 Replacing the Module s Firmware 147 This chapter describes the maintenance of the module ott Agilent Technologies 115 9 Maintenance Introduction to Maintenance and Repair 116 The module is designed for easy repair The most frequent repairs such as piston seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack These repairs are described in Overview of Maintenance on page 119 Agilent 1260 IlsoPump QuatPump User Manual Maintenance 9 Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The hand
81. ing general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation 180 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Agilent 126
82. ing the Purge Valve Frit or the Purge Valve on page 126 Agilent 1260 lsoPump QuatPump User Manual Maintenance 9 Maintenance of a Pump Head with Seal Wash Option When When maintaining seal wash option Tools required 4 mm hexagonal key Parts required p n Description 1 01018 23702 Insert tool 1 0905 1175 Wash seal 1 5062 2484 Gasket seal wash pack of 6 Preparations Switch off pump at the main power switch Remove the front cover Use a solvent shutoff valve or lift up solvent filters for avoiding leakages Remove the pump head see Removing the Pump Head Assembly on page 128 Remove the wash solvent tubings from the support ring inlet and outlet Agilent 1260 IlsoPump QuatPump User Manual 135 9 Maintenance 1 Remove the seal holder and the seal wash support rings from the piston housing Remove the seal holder from the support ring assembly Seal holder Seal wash support ring Piston housing 3 Using the insert tool press the wash seal spring pointing upwards into the recess of the support ring Insert tool Wash seal Support ring 136 2 Using the blade of a flat blade screwdriver remove the seal wash gasket and the secondary seal from the support ring The removed seal will be damaged and cannot be re used Secondary seal 4 Place a seal wash gasket in the recess of the support ring Put the seal holder on top of the gasket Seal Seal holder S
83. innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 18 Agilent 1260 lsoPump QuatPump User Manual Introduction 1 Electrical Connections e The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems The interface board slot is used for external contacts and BCD bottle number output or LAN connections The REMOTE connector may be used i
84. ins technical information about the Agilent 1260 Infinity Isocratic Pump G1310B and Quaternary Pump G1311B The manual describes the following introduction e site requirements and specifications e installing the pump using the pump e optimizing performance e troubleshooting and diagnostics maintenance e parts and materials for maintenance e identifiying cables appendix Agilent Technologies 2010 Printed in Germany 06 10 G1310 90012 EE Agilent Technologies
85. ion 40 41 flowrange 40 41 frequency range 39 G general error messages 90 gradient formation 41 gradient valve 140 hexagonal key 4mm 128 130 135 138 highest injection precision 72 hints for successful use 66 67 humidity 39 hydraulic path 162 163 hydraulic system 40 41 index limit 105 index adjustment 105 index missing 106 initialization failed 107 initialization 14 inlet valve fuse 100 inlet valve missing 102 installation pump module 50 installation bench space 38 instrument layout 18 interface cables 52 interfaces 21 internet 188 introduction to the pump 8 L LAN cable 1 5 leak rate test 114 leak sensor open 93 leak sensor short 93 leak 92 line frequency 39 line voltage 39 lithium batteries 184 M maintenance feedback 17 overview 119 replacing firmware 147 147 materials in contact with mobile phase 14 15 message ignition without cover 95 95 remote timeout 91 Agilent 1260 IsoPump QuatPump User Manual Index missing pressure reading 98 missing parts 44 motor drive power 101 multi channel gradient valve MCGV 140 non operating altitude 39 non operating temperature 39 0 operating Altitude 39 operating temperature 39 operational pressure range 41 optimization stack configuration 47 outlet valve 124 154 overview pump 10 P packaging damaged 44 parts identification cables 165 parts active inlet valve 156 bottle head assembly 161 damaged 44 hydraulic path
86. ions of the isocratic pump see Table 15 on page 40 For specifications of the quaternary pump see Table 16 on page 41 How Does Compressibility Compensation Work The compressibility of the solvents in use will affect retention time stability when the back pressure in the system changes for example ageing of column In order to minimize this effect the pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent type The compressibility compensation is set to a default value and can be changed through the user interface Without a compressibility compensation the following will happen during a stroke of the first piston The pressure in the piston chamber increases and the volume in the chamber will be compressed depending on back pressure and solvent type The volume displaced into the system will be reduced by the compressed volume Agilent 1260 IlsoPump QuatPump User Manual 15 1 16 Introduction With a compressibility value set the processor calculates a compensation volume that depends on the back pressure of the system and the selected compressibility This compensation volume will be added to the normal stroke volume and compensates the previously described loss of volume during the delivery stroke of the first piston How Does Variable Stroke Volume Work Due to the compression of the pump chamber volume each piston stroke of the pump will generate a small pressure
87. is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative Agilent 1260 IsoPump QuatPump User Manual 105 7 Error Information Index Missing The encoder index position in the module was not found during initialization During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is not recognized within a defined time the error message is generated Probable cause Suggested actions 1 Disconnected or defective encoder cable Please contact your Agilent service representative 2 Defective pump drive assembly Please contact your Agilent service representative Stroke Length The distance between the lower piston position and the upper mechanical stop is out of limits pump During initialization the module monitors the drive current If the piston reaches the upper mechanical stop position before expected the motor current increases as the module attempts to drive the piston beyond the mechanical stop This current increase causes the error message to be generated Probable cause Suggested actions 1 Defective pump
88. laced above the pump in the solvent cabinet and the peristaltic pump moves a flow through the pump head removing all possible buffer crystals from the back of the pump seal Wash seals running dry Running dry is the worst case for a wash seal and drastically reduces its lifetime The seal will build up sticky layers on the surface of the piston These sticky layers will also reduce the lifetime of the pump seal gt The tubes of the wash option should always be filled with solvent to prolong the lifetime of the wash seal gt Always use a mixture of LC grade water 90 and isopropanol 10 as wash solvent This mixture prevents growth of algae or bacteria in the wash bottle and reduces the surface tension of the water 74 Agilent 1260 lsoPump QuatPump User Manual Optimiziming Performance 5 When to Use the Seal Wash Option Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent The seal wash option is strongly recommended when buffer concentrations of 0 1 M or higher will be used for long time periods in the pump The active seal wash upgrade can be ordered as G1398A The seal wash option comprises a support ring secondary seal gasket and seal holder for both piston sides A wash bottle filled with water isopropanol 90 10 is placed above the pump in the sol
89. lation Isopropanol Best solvent to flush air out of When switching between lsopropanol the system reverse phase and normal Miscible with almost all phase both times solvents After an installation To clean the system when using buffers After changing aqueous solvents After the installation of normal phase seals P N 0905 1420 Ethanol or methanol HPLC grade water HPLC grade water Hexane 5 isopropanol Alternative to isopropanol second choice if no isopropanol is available Best solvent to re dissolve buffer crystals Best solvent to re dissolve buffer crystals Good wetting properties Agilent 1260 IlsoPump QuatPump User Manual 63 3 Installing the Pump Priming the System 64 Agilent 1260 lsoPump QuatPump User Manual bd Agilent 1260 lsoPump QuatPump User Manual ee 4 Using the Pump e j e i Hints for Successful Use of the Isocratic Pump 66 e Prevent Blocking of Solvent Filters 68 Algae Growth in HPLC Systems 69 How to Prevent and or Reduce the Algae Problem 70 This chapter explains the operational parameters of the module gy Agilent Technologies 65 4 Using the Pump Hints for Successful Use of the Isocratic Pump 66 Always place solvent cabinet with the solvent bottle s on top of the pump or at a higher level When using salt solutions and organic solvents in the Agilent 1260 Infinity Quaternary Pump it is recommended to connect the salt solution to one
90. le cause Suggested actions 1 Volume in bottle below specified volume Refill bottles and reset solvent counters 2 Incorrect setting Make sure the limits are set correctly Agilent 1260 lsoPump QuatPump User Manual Error Information 7 Pressure Above Upper Limit The system pressure has exceeded the upper pressure limit Probable cause Suggested actions 1 Upper pressure limit set too low Ensure the upper pressure limit is set to a value suitable for the analysis 2 Blockage in the flowpath after the damper Check for blockage in the flowpath The following components are particularly subject to blockage inline filter frit needle autosampler seat capillary autosampler sample loop autosampler column frits and capillaries with small internal diameters e g 50 um ID 3 Defective damper Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative Pressure Below Lower Limit The system pressure has fallen below the lower pressure limit Probable cause Suggested actions 1 Lower pressure limit set too high Ensure the lower pressure limit is set to a value suitable for the analysis 2 Air bubbles in the mobile phase Ensure solvents are degassed Purge the module Ensure solvent inlet filters are not blocked 3 Leak Inspect the pump head capillaries and fittings for signs of a leak Purge the module Run a pressure test to determine w
91. ling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The amount of substances should be reduced to the minimal volume required for the analysis gt Do not operate the instrument in an explosive atmosphere WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the metal top cover of the module No serviceable parts inside gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides Agilent 1260 IlsoPump QuatPump User Manual 117 9 Maintenance Warnings and Cautions CAUTION Safety standards for external equipment gt If you connect external equipment
92. low path blockage in front of the damper 2 Blocked passive inlet valve 3 Blocked outlet valve 4 High friction partial mechanical blockage in the pump drive assembly 5 Defective pump drive assembly 6 Defective main board Agilent 1260 IsoPump QuatPump User Manual Suggested actions Ensure the capillaries and frits between the pump head and damper inlet are free from blockage Exchange the passive inlet valve Exchange the outlet valve Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly Please contact your Agilent service representative Please contact your Agilent service representative 101 7 102 Error Information Encoder Missing The optical encoder on the pump motor in the module is missing or defective The processor checks the presence of the pump encoder connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable cause 1 Defective or disconnected pump encoder connector 2 Defective pump drive assembly Inlet Valve Missing Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative The active inlet valve in the module is missing or defective The processor checks the presence of the active inlet valve connector every 2 seconds If the connector is not detected by the processor the er
93. mber is pressed through the outlet valve into the second pump chamber The second piston draws in half of the volume displaced by the first piston and the remaining half volume is directly delivered to the system During the drawing stroke of the first piston the second piston delivers the drawn volume to the system Quaternary pump For solvent compositions from the solvent bottles A B C D the controller divides the length of the intake stroke in certain fractions in which the gradient valve connects the specified solvent channel to the pump input Table 1 _Isocratic pump details Dead volume 800 1100 uL depending on back pressure Materials in contact with mobile phase Pump head SST gold sapphire ceramic Active inlet valve SST gold sapphire ruby ceramic PTFE Outlet valve SST gold sapphire ruby Adapter SST gold Purge valve SST gold PTFE ceramic PEEK Degasser chamber TFE PDD Copolymer FEP PEEK PPS Agilent 1260 lsoPump QuatPump User Manual Introduction 1 Table 2 Quaternary Pump Details Delay volume 800 1100 uL dependent on back pressure Materials in contact with mobile phase MCGV PTFE Pump head SST gold sapphire ceramic Active inlet valve SST gold sapphire ruby ceramic PTFE Outlet valve SST gold sapphire ruby Adapter SST gold Purge valve SST gold PTFE ceramic PEEK Damping unit Gold SST Degasser chamber TFE PDD Copolymer FEP PEEK PPS For specificat
94. mpensation Setting 77 Agilent 1260 IlsoPump QuatPump User Manual Contents 6 Troubleshooting and Diagnostics 79 Overview of the Module s Indicators and Test Functions 80 Status Indicators 82 User Interfaces 84 Agilent Lab Advisor Software 85 7 Error Information 87 What Are Error Messages 89 General Error Messages 90 Module Error Messages 96 8 Test Functions and Calibration 111 Introduction 112 System Pressure Test 113 Leak Rate Test 114 9 Maintenance 115 Introduction to Maintenance and Repair 116 Warnings and Cautions 117 Overview of Maintenance 119 Cleaning the Module 120 Checking and Cleaning the Solvent Filter 121 Exchanging the Passive Inlet Valve PIV 123 Exchanging the Outlet Valve 124 Exchanging the Purge Valve Frit or the Purge Valve 126 Removing the Pump Head Assembly 128 Maintenance of a Pump Head Without Seal Wash Option 130 Seal Wear in Procedure 134 Maintenance of a Pump Head with Seal Wash Option 135 Reinstalling the Pump Head Assembly 138 Exchanging the Multi Channel Gradient Valve MCGV 140 Exchanging the Optional Interface Board 143 Exchanging the Active Inlet Valve AIV or its Cartridge 145 Replacing the Module s Firmware 147 Agilent 1260 IsoPump QuatPump User Manual Contents 10 Parts for Maintenance 149 Pump Head Assembly Without Seal Wash 150 Pump Head Assembly with Seal Wash Option 600 bar 152 Outlet Valve 154 Purge Valve Assembly 155 Active Inlet Valve Assembly 1
95. n combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch e The power input socket accepts a line voltage of 100 240 VAC 10 witha line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1260 IlsoPump QuatPump User Manual 19 1 Introduction Serial Number Information ALL The serial number information on the instrument labels provide the following information CCXZZ00000 Format CC Country of manufacturing DE Germany X Alphabetic character A Z used by manufacturing ZZ Alpha numeric code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same module 00000 Serial number Rear View of the Module
96. n damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1260 lsoPump QuatPump User Manual Contents Contents 1 Introduction 7 Introduction tothe Pump 8 Overview of the Hydraulic Path 10 Early Maintenance Feetback 17 Instrument Layout 18 Electrical Connections 19 Interfaces 21 Setting the 8 bit Configuration Switch 27 2 Site Requirements and Specifications 35 Site Requirements 36 Physical Specifications 39 Performance Specifications 40 3 Installing the Pump 43 Unpacking the Pump 44 Optimizing the Stack Configuration 47 Installing the Pump 50 Connecting Modules and Control Software 53 Flow Connections of the Pump 56 Priming the System 59 4 Usingthe Pump 65 Hints for Successful Use of the Isocratic Pump 66 Prevent Blocking of Solvent Filters 68 Algae Growth in HPLC Systems 69 5 Optimiziming Performance 71 When to Use a Vacuum Degasser 72 Operational Hints for the Multi Channel Gradient Valve MCGV 73 When to use the Seal Wash Option 74 Choosing the Right Pump Seals 76 Optimize the Compressibility Co
97. nalytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3394 Pin Agilent Signal Name Active module TTL Z 9 1 White Digital ground a NC 2 Brown Prepare run Low 3 3 Gray Start Low z NC 4 Blue Shut down Low 5 NC 5 Pink Not connected i NC 6 Yellow Power on High o 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators p n 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready Agilent 1260 lsoPump QuatPump User Manual Identifying Cables 11 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground gt NC 2 Brown Prepare run Low e 3 3 Gray Start Low m s NC 4 Blue Shut down Low e O a NC 5 Pink Not connected NC 6 Yellow Power on High o 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 A D Pin Agilent Signal Name Active module TTL 1 White 1
98. ng a Vacuum Degasser The quaternary pump has a built in degasser For the isocratic pump an external degasser can be used and a pump upgrade to the quaterary pump including a built in degasser is possible Place the vacuum degasser in the stack of modules as shown in Figure 10 on page 48 Connect the bottle head assembly in the solvent reservoir to the degasser inlet Connect the degasser outlet to the inlet valve of the pump Ensure the power switch at the front of the vacuum degasser is OFF switch stands out Plug an APG cable into the APG remote connector at the rear of the degasser Connect the APG cable to the APG remote connector of the pump see Figure 11 on page 49 Press in the power switch to turn on the vacuum degasser The AUX output is intended for troubleshooting It provides a DC voltage in the range of 0 1 V which is proportional to the vacuum level in the degasser chambers Agilent 1260 lsoPump QuatPump User Manual Installing the Pump 3 Connecting Control Software and or G4208 A Instant Pilot With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN Usually the detector is producing the most data in the stack followed by the pump and it is therefore highly recommended to use either of these modules for the LAN connection 1 Ensure the power switches on the front of the modules in the stack are OFF switches stand out 2 If the
99. nt Pilot with which the pressure and ripple can be monitored otherwhise connect a signal cable between the pressure output of the pump and a recording device for example 339X integrator and set parameters Zero 50 Att 2 3 Chart Speed 10 cm min 4 Start the recording device with the plot mode 5 Starting with a compressibility setting of 10 x 10 bar increase the value in steps of 10 Re zero the integrator as required The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition Agilent 1260 IlsoPump QuatPump User Manual 77 5 78 Optimiziming Performance Table 19 Solvent Compressibility Solvent pure Compressibility 10 6 bar Acetone Acetonitrile Benzene Carbon tetrachloride Chloroform Cyclohexane Ethanol Ethyl acetate Heptane Hexane Isobutanol lsopropanol Methanol 1 Propanol Toluene Water 126 115 95 110 100 118 114 104 120 150 100 100 120 100 87 46 Agilent 1260 lsoPump QuatPump User Manual Agilent 1260 lsoPump QuatPump User Manual 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 80 Status Indicators 82 Power Supply Indicator 82 Module Status Indicator 83 User Interfaces 84 Agilent Lab Advisor Software 85 This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces ee Agilent Technologies
100. ntative Valve Failed MCGV SSV Valve 0 Failed valve A Valve 1 Failed valve B Valve 2 Failed valve C Valve 3 Failed valve D One of the valves of the multi channel gradient valve has failed to switch correctly The processor monitors the valve voltage before and after each switching cycle If the voltages are outside expected limits the error message is generated Probable cause Suggested actions 1 Gradient valve disconnected Ensure the gradient valve is connected correctly 2 Connection cable inside instrument not Please contact your Agilent service connected representative 100 Agilent 1260 IlsoPump QuatPump User Manual Probable cause 3 Connection cable inside instrument defective 4 Gradient valve defective Motor Drive Power Error Information 7 Suggested actions Please contact your Agilent service representative Exchange the gradient valve The current drawn by the pump motor exceeded the maximum limit Blockages in the flow path are usually detected by the pressure sensor in the damper which result in the pump switching off when the upper pressure limit is exceeded If a blockage occurs before the damper the pressure increase cannot be detected by the pressure sensor and the module will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the module is switched off and the error message is generated Probable cause 1 F
101. ocked only very little solvent will drip out of the solvent tube Agilent 1260 IlsoPump QuatPump User Manual 121 9 Maintenance Cleaning the Solvent Filter When If solvent filter is blocked Parts required Description Concentrated nitric acid 35 LC grade water Beaker Preparations Remove solvent inlet tube from the adapter at the inlet valve 1 Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 85 for one hour 2 Thoroughly flush the filter with LC grade water remove all nitric acid some columns can be damaged by concentrated nitric acid 3 Reinstall the filter 122 Agilent 1260 IlsoPump QuatPump User Manual Maintenance 9 Exchanging the Passive Inlet Valve PIV When Tools required Parts required If internally leaking backflow Wrench 14 mm Pair of Tweezers Description G1312 60066 Passive inlet valve Remove the front cover Disconnect the solvent inlet tube from the inlet valve be aware that solvent may leak out of the tube due to hydrostatic flow Using a 14 mm wrench loosen the passive inlet valve and remove the valve from the pump head Figure 16 Passive Inlet Valve Insert the new valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight Using the 14 mm wrench tighten the nut by turning the valve in its final position not more than a quarter turn Reconnect the solvent inlet t
102. of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the salt solution channel Regular flushing with water of all MCGV channels is recommended to remove all possible salt deposits in the valve ports Before operating the pump flush the vacuum degasser with at least two volumes 30 mL especially when turned off for a certain length of time for example during the night and volatile solvent mixtures are used in the channels see Regular Priming on page 61 Prevent blocking of solvent inlet filters never use the pump without solvent inlet filter Growth of algae should be avoided see Prevent Blocking of Solvent Filters on page 68 Check purge valve frit and column frit in regular time intervals A blocked purge valve frit can be identified by black or yellow layers on its surface or by a pressure greater than 10 bar when pumping distilled water at a rate of 5 mL min with an open purge valve When using the pump at low flow rates for example 0 2 mL min check all 1 16 inch fittings for any signs of leaks Always exchange the purge valve frit too when exchanging the seals When using buffer solutions flush the system with water before switching it off The seal wash option should be used when buffer solutions of 0 1 M or higher will be used for long time periods Check the pump plungers for scratches when changing th
103. on Preparations Switch off pump at the main power switch Remove the front cover Removing the Pump Head Assembly on page 128 130 Agilent 1260 IlsoPump QuatPump User Manual Maintenance 9 Maintenance of a Pump Head Without Seal Wash Option 1 Place the pump head on a flat surface Loosen the lock screw two revolutions and while holding the lower half of the assembly carefully pull the pump head away from the piston housing Agilent 1260 IlsoPump QuatPump User Manual 2 Remove the support rings from the piston housing and lift the housing away from the pistons Support ring Piston housing Piston 131 9 Maintenance Maintenance of a P imp Head Without Seal Was 3 Check the piston surface and remove any deposits or layers Cleaning can be done with alcohol or tooth paste Replace piston if scratched Piston surface 5 Insert new seals into the pump head Pump seals 4 Using the insert tool carefully remove the seal from the pump head Remove wear retainers if still present Insert tool Pump head ye Support ring Piston housing 132 Agilent 1260 IsoPump QuatPump User Manual Maintenance 9 Next Steps 7 Ifa standard seal has been installed run the seal wear in procedure see Seal Wear in Procedure on page 134 8 For the normal phase seal the purge valve frit should be replaced see Exchanging the Pur
104. ostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues Agilent 1260 IlsoPump QuatPump User Manual 85 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 86 Agilent 1260 lsoPump QuatPump User Manual Agilent 1260 lsoPump QuatPump User Manual 7 Error Information What Are Error Messages 89 General Error Messages 90 Timeout 90 Shut Down 90 Remote Timeout 91 Synchronization Lost 92 Leak 92 Leak Sensor Open 93 Leak Sensor Short 93 Compensation Sensor Open 94 Compensation Sensor Short 94 Fan Failed 95 Open Cover 95 Module Error Messages 96 Restart Without Cover 96 Solvent Zero Counter 96 Pressure Above Upper Limit 97 Pressure Below Lower Limit 97 Pressure Signal Missing 98 Missing Pressure Reading 98 Pump Configuration 99 Valve F
105. p run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces Some modules have module specific interfaces connectors They are described in the module documentation Agilent 1260 lsoPump QuatPump User Manual Introduction 1 Setting the 8 bit Configuration Switch Setting the 8 bit Configuration Switch On Board LAN The 8 bit configuration switch is located at the rear of the module Switch settings provide configuration parameters for LAN serial communication protocol and instrument specific initialization procedures All modules with on board LAN e g G1315 65C D G1314D E F G4212A B G4220A Default is ALL switches DOWN best settings Bootp mode for LAN For specific LAN modes switches 3 8 must be set as required e For boot test modes switches 1 2 must be UP plus required mode il REMOTE oP eg Se ese l CONFIG CAN _ CAN a RE 282 USB LAN g al HOOCOL C E gt C gt C C pia gt gt E Agilent Technologies 7 gt gt gt gt c c ji b
106. ping out of waste outlet when valve closed A pressure drop of gt 10 bar across the frit 5 mL min H30 with purge open indicates blockage Leaks at lower pump head side unstable retention times pressure ripple unstable run Leak Rate Test for verification Seal life time shorter than normally expected check pistons while changing the seals Error condition indicated by red status indicator Agilent 1260 IlsoPump QuatPump User Manual 119 9 Maintenance Cleaning the Module The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth as liquid may drip into the module WARNING Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings 120 Agilent 1260 IlsoPump QuatPump User Manual Maintenance 9 Checking and Cleaning the Solvent Filter CAUTION Small particles can permanently block the capillaries and valves of the module Damage of the module gt Always filter solvents gt Never use the module without solvent inlet filter If the filter is in good condition the solvent will freely drip out of the solvent tube hydrostatic pressure If the solvent filter is partly bl
107. pulsation influencing the flow stability of the pump The amplitude of the pressure pulsation depends mainly on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes generate pressure pulsations of smaller amplitude than higher stroke volumes at the same flow rate In addition the frequency of the pressure pulsations is higher This decreases the influence of flow pulsations on quantitative results In gradient mode smaller stroke volumes result in a lower flow ripple improve composition ripple The module uses a processor controlled spindle system for driving its pistons The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume By default the stroke volume for the pump is set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended Agilent 1260 lsoPump QuatPump User Manual Introduction 1 Early Maintenance Feetback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedb
108. r to achieve a low pressure ripple see Regular Priming on page 61 146 Agilent 1260 IsoPump QuatPump User Manual Maintenance 9 Replacing the Module s Firmware When The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third part control software requires a special version Tools required LAN RS 232 Firmware Update Tool or Agilent Diagnostic Software Instant Pilot G4208A only if supported by module Parts required Description 1 Firmware tools and documentation from Agilent web site Preparations Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 To load the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module Agilent 1260 IlsoPump QuatPump User Manual 147 9 Maintenance Replacing the Module s Firmware 148 Agilent 1260 IlsoPump QuatPump User Manual
109. re are no other 1260 with LAN port in the HPLC stack install a G1369B LAN board into the extension slot of the pump 3 Connect the LAN enabled module with a LAN cable to the data system 4 Plug the CAN connector of the Instant Pilot into any available CAN port of the 1260 system 5 Plug a CAN cable into the CAN connector of the Instant Pilot 6 Connect the CAN cable to the CAN connector of one of the modules 7 Press in the power switches to turn on the modules The Agilent control software can also be connected to the system through a LAN cable which requires the installation of a LAN board For more information about connecting the Instant Pilot or Agilent control software refer to the respective user manual For connecting the Agilent 1260 Infinity equipment to non Agilent 1260 Infinity equipment see Introduction to the Quaternary Pump on page 8 Agilent 1260 IlsoPump QuatPump User Manual 55 3 Installing the Pump Flow Connections of the Pump Parts required Description 1 Other modules 1 Parts from accessory kit 2 wrenches 1 4 5 16 inch for capillary connections Preparations Pump is installed in the LC system WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing
110. ror message is generated Probable cause 1 Disconnected or defective cable 2 Disconnected or defective connection cable front panel to main board 3 Defective active inlet valve Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Exchange the active inlet valve Agilent 1260 lsoPump QuatPump User Manual Error Information 7 Temperature Out of Range The temperature sensor readings in the motor drive circuit are out of range The values supplied to the ADC by the hybrid sensors must be between 0 5 V and 4 3 V If the values are outside this range the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Temperature Limit Exceeded The temperature of one of the motor drive circuits is too high The processor continually monitors the temperature of the drive circuits on the main board If excessive current is being drawn for long periods the temperature of the circuits increases If the temperature exceeds the upper limit the error message is generated Probable cause Suggested actions 1 High friction partial mechanical blockage in Ensure the capillaries and frits between the the pump drive assembly pump head and damper inlet are free from blockage 2 Partial blockage of the flowpath in front of Ensure the outlet valve is not blocked the damper
111. s Yes Yes Yes No No Yes No No No No No Yes Yes Yes Yes Yes Yes Yes Yes Yes No No No No No Yes Yes Yes CAN DC OUT for CAN slaves Yes THERMOSTAT for G1330B Yes THERMOSTAT for G1330B CAN DC OUT for CAN slaves Yes Yes Yes Agilent 1260 IsoPump QuatPump User Manual 21 1 introduction Table3 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4212A B DAD 2 No Yes Yes 1 Yes G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 1 Yes G1362A RID G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1316A C TCC 2 No No Yes No Yes G1322A DEG No No No No No Yes AUX G1379B DEG No No No Yes No No AUX G4227A Flex Cube 2 No No No No No G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED 22 The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN CAN connectors as interface to other modules LAN connector as interface to the control software e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output Agilent 1260 lsoPump QuatPump User Manual Introduction 1 Overview Interfaces CAN
112. s 1 60 niin coq IERE Ca Figure 25 Hydraulic Flow Path of the Isocratic Pump 162 Agilent 1260 IlsoPump QuatPump User Manual Hydraulic Path Item p n 1 G1312 67305 G1329 87300 G1311 60003 G1322 67300 G1311 81600 G1311 81601 5067 4693 G1311 67304 5062 2461 Parts for Maintenance 10 Description Outlet capillary pump to injector device or Outlet capillary pump to thermostattable autosampler Bottle head assembly Kit of 4 solvent tubes for connection degasser to MCGV Quaternary Pump including labels Capillary piston 1 to damper Capillary damper to piston 2 Connecting tube MCGV to PIV or Connecting tube MCGV to AIV Waste tube 5 m reorder pack Agilent 1260 IlsoPump QuatPump User Manual 163 10 Parts for Maintenance vdraulic Path Figure 26 Hydraulic Flow Path of the Quaternary Pump 164 Agilent 1260 IlsoPump QuatPump User Manual Agilent 1260 lsoPump QuatPump User Manual 11 Identifying Cables Cable Overview 166 Analog Cables 168 Remote Cables 170 BCD Cables 173 CAN LAN Cables 175 External Contact Cable 176 Agilent Module to PC 177 Agilent 1200 Module to Printer 178 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules ot
113. serve appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used Agilent 1260 IlsoPump QuatPump User Manual 181 12 Appendix Safety Symbols Table 23 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the A instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced J by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up EAE es alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 182 Agilent 1260 IsoPump QuatPump User Manual Appendix 12 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE
114. t gt c C gt 2 gt gt A C 3 l 3 deen ay amwr A f Figure 8 Location of Configuration Switch example shows a G4212A DAD To perform any LAN configuration SW1 and SW2 must be set to OFF For details on the LAN settings configuration refer to chapter LAN Configuration Agilent 1260 IsoPump QuatPump User Manual 27 1 introduction Table6 8 bit Configuration Switch with on board LAN Mode Function SW 1 SW 2 SW 3 SW 4 SW 5 SW 6 SW7 SW 8 LAN 0 0 Link Configuration Init Mode Selection Auto negotiation 0 xX x x x x 10 MBit half duplex 1 0 0 X X xX 10 MBit full duplex 1 0 1 xX x xX 100 MBit half duplex 1 1 0 x X X 100 MBit full duplex 1 1 1 Xx x xX Bootp x xX x 0 0 0 Bootp amp Store Xx X X 0 0 1 Using Stored x X x 0 1 0 Using Default Xx xX xX 0 1 1 TEST 1 1 System NVRAM Boot Resident System 1 X Revert to Default Data Coldstart X X X 1 Legend 0 switch down 1 switch up x any position When selecting the mode TEST the LAN settings are Auto Negotiation amp Using Stored For explanation of Boot Resident System and Revert to Default Data Coldstart refer to Special Settings on page 32 28 Agilent 1260 lsoPump QuatPump User Manual Introduction 1 Setting the 8 bit Configuration Switch without On Board LAN The 8 bit configuration switch is located at the rear of the module Modules that do not have their own LAN interface
115. t Agilent Technologies 165 11 Identifying Cables Cable Overview 166 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n Description 35900 60750 Agilent module to 3394 6 integrators 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs Remote cables p n Description 03394 60600 Agilent module to 3396A Series integrators 3396 Series Il 3395A integrator see details in section Remote Cables on page 170 03396 61010 Agilent module to 3396 Series III 3395B integrators 5061 3378 Agilent module to Agilent 35900 A D converters or HP 1050 1046A 1049A 01046 60201 Agilent module to general purpose BCD cables p n Description 03396 60560 Agilent module to 3396 integrators G1351 81600 Agilent module to general purpose Agilent 1260 IlsoPump QuatPump User Manual Identifying Cables 11 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable Agilent module interface board to general purposes RS 23
116. the pump from line power Disconnect cables from the interfaceboard connectors Loosen the screws Slide out the interface board from the pump Install the new interface board Secure screws oF W N Reconnect the cables to the board connector Agilent 1260 IlsoPump QuatPump User Manual 143 9 Maintenance Exchanging the Optional Interface Board 6 Reconnect the pump to line power BCD interface board Figure 17 Exchanging the Interface Board 144 Agilent 1260 IlsoPump QuatPump User Manual Maintenance 9 Exchanging the Active Inlet Valve AIV or its Cartridge When Tools required Parts required Preparations If internally leaking backflow Wrench 14mm Pair of Tweezers p n Description 1 G1312 60025 Active inlet valve body optional without cartridge 1 G1312 60020 Cartridge for active inlet valve 600bar 1 G1311 67304 Connecting tube MCGV to AIV Switch off pump at the main power switch and unplug the power cable Use a solvent shutoff valve or lift up solvent filters for avoiding leakages The active inlet valve can be installed for highest method backward compatibility or special applications This is a configuration change which is not covered by the specifications for this module By default 1260 Infinity pumps do not have an active inlet valve The first time an AIV shall be installed a connector and cable to the main board must be installed by the service and a different connection tu
117. tion Tools required Wrench 1 4 inch 4 mm hexagonal key Preparations Switch off pump at the main power switch and unplug the power cable Use a solvent shutoff valve or lift up solvent filters for avoiding leakages CAUTION Damage of the pump drive Starting the pump when the pump head is removed may damage the pump drive gt Never start the pump when the pump head is removed 1 Remove the front cover 2 Ifan active inlet valve is installed disconnect the active inlet valve cable 3 Using a 1 4 inch wrench remove the outlet capillary Outlet valve capillary Outlet capillary Waste tubing 128 Agilent 1260 lsoPump QuatPump User Manual Maintenance 9 Next Steps 7 Using a 4 mm hexagonal key stepwise loosen the two pump head screws and remove the pump head from the 4 Disconnect the capillary from the outlet valve pump drive Remove the waste tubing and disconnect the solvent tubing from the inlet valve 6 Remove the capillary at the bottom of the pump head Pump head screws Agilent 1260 IlsoPump QuatPump User Manual 129 9 Maintenance Maintenance of a Pump Head Without Seal Wash Option When In case of maintenance or pump head internal leaks Tools required Wrench 1 4 inch 4 mm hexagonal key Parts required p n Description 1 01018 23702 Insert tool 1 5063 6589 Standard seals pack of 2 1 or 1 0905 1420 PE seals pack of 2 1 5063 6586 Pist
118. ube to the passive inlet valve Reinstall the front cover Agilent 1260 IsoPump QuatPump User Manual 123 9 Maintenance Exchanging the Outlet Valve When If internally leaking Tools required Wrench 1 4 inch Wrench 14 mm Parts required Description G1312 60067 Outlet valve complete Preparations Switch off pump at the main power switch Remove the front cover Before exchanging the outlet valve you can try to clean it in a sonic bath for 5 10 min Place the valve in an upright position in a small beaker with alcohol 1 Using a 1 4 inch wrench disconnect the valve capillary 3 Check that the new valve is assembled correctly from the outlet valve 2 Using the 14 mm wrench loosen the valve and remove it from the pump body 124 Agilent 1260 lsoPump QuatPump User Manual Maintenance 9 Exchanging the Outlet Valve 4 Reinstall the outlet valve and tighten the valve 5 Reconnect the valve capillary Valve capillary 2 capillary Outlet valve Agilent 1260 IsoPump QuatPump User Manual 125 9 Maintenance Exchanging the Purge Valve Frit or the Purge Valve When Frit when piston seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across the frit at a flow rate of 5 mL min of H20 with purge valve opened Purge valve if internally leaking Tools required Wrench 1 4 inch Wrench 14mm e Pair of tweezers or toothpick p n Description
119. umbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 184 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA Agilent 1260 IsoPump QuatPump User Manual Appendix 12 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent 1260 IlsoPump QuatPump User Manual 185 12 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 186 Agilent 1260 IlsoPump QuatPump User Manual Appendix 12 Solvent Information Observe the following recommendations on the use of solvents e Brown glass ware can avoid growth of algae e Small particles can perm
120. ump QuatPump User Manual Index Index AUX output 54 solvent zero counter 96 B ball screw drive 12 configuration bat 7 l P one stack 47 safety information BCD 8 cable 173 bench space 38 blockage 101 bottle head assembly 161 buffer application 66 67 8 bit configuration switch on board LAN 27 without On Board LAN 29 A buffer solution 8 140 active inlet valve cartridge 145 c active inlet valve 145 156 active seal wash 8 74 75 on analog 168 adapter 145 BCD 173 Agilent Diagnostic software 85 CAN 175 Agilent Lab Advisor software 85 external contact 176 Agilent Lab Advisor 85 interface 52 Agilent LAN 175 on internet 188 remote 170 algae growth 68 RS 232 177 algae 66 187 cables alternative seal material 76 analog 166 ambient operating temperature 39 BCD 166 ambient non operating temperature 39 CAN 167 analog output 40 external contact 167 analog signal 24 LAN ba overview 166 analog 68 remote 166 ae 4 RS 232 167 apgremote 25 CAN AUTO mode 16 cable 175 Agilent 1260 IlsoPump QuatPump User Manual cleaning 120 Communication settings RS 232C 30 compensation sensor open 94 compensation sensor short 94 composition precision 41 composition range 41 compressibility compensation 15 40 41 77 condensation 38 configuration one stack 47 connections flow 56 control software 55 D damaged parts 44 defect on arrival 44 delay volume 14 15 41 delivery checklist 44 Diagnostic software 85 dimensions
121. use 99 Inlet Valve Fuse 100 Valve Failed MCGV SSV 100 Motor Drive Power 101 Encoder Missing 102 Inlet Valve Missing 102 Temperature Out of Range 103 Re Agilent Technologies 87 7 88 Error Information Temperature Limit Exceeded 103 Servo Restart Failed 104 Pump Head Missing 104 Index Limit 105 Index Adjustment 105 Index Missing 106 Stroke Length 106 Initialization Failed 107 Wait Timeout 107 Degasser cannot read signal 108 Degasser limit notreached 109 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions Agilent 1260 lsoPump QuatPump User Manual Error Information 7 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook Agilent 1260 IlsoPump QuatPump User Manual 89 7 Error Information General Error Messages 90 General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well The timeout threshold was exceeded Probable cause 1 The analysis was completed successfully and th
122. vent cabinet and the peristaltic pump moves a flow through the pump head removing all possible buffer crystals from the back of the pump seal Running dry is the worst case for a wash seal and drastically reduces its lifetime The seal will build up sticky layers on the surface of the piston These sticky layers will also reduce the lifetime of the pump seal Therefore the tubes of the wash option should always be filled with solvent to prolong the lifetime of the wash seal Always use a mixture of LC grade water 90 and isopropanol 10 as wash solvent This mixture prevents growth of algae or bacteria in the wash bottle and reduces the surface tension of the water Agilent 1260 IlsoPump QuatPump User Manual 75 5 Optimiziming Performance Choosing the Right Pump Seals The standard seal for the pump can be used for most applications However applications that use normal phase solvents for example hexane are not suited for the standard seal and require a different seal when used for a longer time in the pump For applications that use normal phase solvents for example hexane we recommend using polyethylene pump seals PE seals pack of 2 p n 0905 1420 and Wash Seal PE p n 0905 1718 These seals have less abrasion compared to the standard seals Polyethylene seals have a limited pressure range of 0 200 bar When used above 200 bar their lifetime is reduced significantly DO NOT apply the seal wear in procedure perform

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