User Manual 620M
Contents
1. Myo Interface display Heat indicator 77 Valve buttons Power indicator Figure 1 1 Interface Front Panel 1 2 Interface Rear Panel 4 Transducer connections Gas regulator needle valve E Gas input IRANS 2 9 OXYGEN ON OFF e POWER switch RX TX US B VACUUM 24vDC 52 e TT d v REC 4 M Power connector 4 Recorder outputs Temperature probe Vacuum input Grounding connected to USB output RS 232 Port for serial Data Acquisition connection to PC Figure 1 2 Interface Rear Panel CHAPTER1 11 1 3 Multi wire myograph unit Allen screws for fine alignment of the e Connection to Myo Interface myograph jaws Micropositioner Figure 1 3 Multi Wire Myograph unit Force transducer pin Myograph jaw connected to micropositioner Myograph jaw connected to force transducer e Figure 1 4 Close up of myograph jaws Figure 1 5 Mounting jaws for small vessels Figure 1 6 Mounting pins for larger vessels 12 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL CHAPTER 2 SETTING UP 2 1 The Complete Myograph 620M System DMT CS200 Pulse Train Stimulator optional PC data acquisition and analysis software optional r T S xn t i 113 l i l i 1 I 1 1 1 I 1 1 1 I 1 1 I2. 1 Remove the pin or jaw from the transducer pin coming out of the transducer house 2 Disconnect the Myograph Chamber from the Interface 3 Turn the Myograph Chamber upside down and remove the transducer housing by loosening the two screws A B as illus trated in Figure 4 9 below A B Figure 4 9 The 2 screws that secure the transducer house to the chamber 4 The replacement transducer will be shipped with the new transducer inside a new transducer house 5 Place a small amount of vacuum grease clear or whitish grease around the bottom of the transducer housing to seal the transducer housing when put back in place 6 Carefully realign the transducer housing with the new transducer on the Myograph Chamber and reinsert the allen screws through the bottom of the Myograph Chamber f Tighten the screws and place some vacuum grease around the transducer pin that protrudes from the transducer housing Make sure that the hole is completely sealed to prevent buffer solution or water from entering the transducer housing and damaging the new force transducer IMPORTANT NOTE CALIBRATE THE NEW FORCE TRANSDUCER BEFORE PERFORMING A NEW EXPERIMENT AS DESCRIBED IN CHAPTER 3 AND 4 2 CHAPTER4 29 4 5 Myograph Maintenance The Multi Wire Myograph System Model 620M is a very delicate and sophisticated piece of research equipment DMT recom mends that the following sections are read carefully and that the instructions are followed at all times
3. C Probe temperature 36 8 C BACK HEAT ON ors NEXT STEP Step no ODOR 5 s Turn the heat on Wait until the temperature is stable Temperature set point 37 0 C Probe temperature 36 8 C BACK NEXT STEP Step GJ s 5 Make sure that the transducer is not subjected to any force When the relative force reading is stable go to next step Force Chamber 1 3261 BACK NEXT STEP Step no JIM Carefully place the 2 g weight On the pan When the relative force reading is stable go to next step Force Chamber 1 3346 NEXT STEP CHAPTER3 19 Step 6 is to verify that the calibration was performed correctly The Force Chamber 1 reading should be 9 81 0 1 mN If the Force Chamber 1 reading is off by more than 0 1 mN then remove the weight press BACK to return to Step 4 and repeat the calibration process If the Force Cham ber 1 reading is satisfactory then press NEXT STEP Calibrate the other chambers in the same manner 2 Valve delay Pressing VALVE DELAY in the SETTINGS menu will allow the user to modify the time duration that the vacuum valves stay open for washes Factory default is set at 1 second but 1 second is not enough time to completely empty a chamber with even as small a volume of 5 ml Pressing SELECT next to any given channel will cause the line selected to turn blue The up and down arrow keys can then be
4. MULTI WIRE MYOGRAPH SYSTEM MODEL 620M 11 1 Nex b 5 EN Empty valves 2 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL MULTI WIRE MYOGRAPH SYSTEM MODEL 620M TRADEMARKS Pentium is a registered trademark of the Intel Corporation Windows Windows 95 Windows 98 Windows ME Windows NT Windows 2000 Windows XP Windows Vista and Windows 7 are registered trademarks of Microsoft Corporation All other trademarks are the properties of their respective owners DMT reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with the DMT Multi Wire Myograph System Model 620M All rights reserved No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A S Every attempt is made to ensure accurate information misprints construction and specification changes can occur Danish Myo Technology A S reserves the right to alter change content as required and without any notice Copyright Danish Myo Technology A S DANISA MYO TECHNOLOG TRADEMARKS 3 Contents TE CUS NAS oe pe ee ie ce a nee set ie ce ee anise eee ec we pm
5. Myograph chamber tubing To prevent the tubing from becoming blocked with buffer salt deposits after an experiment remove the chamber cover from the Myograph Chamber and turn on the vacuum and press the vacuum valve for about 10 seconds by holding down the valve button s down Turn off the vacuum and gas supply Remove any water or buffer remaining in the chamber or on the tubing using absorbent paper Force transducer The force transducer is the most delicate and fragile component of the myograph system Extreme care must be used when handling or touching the force transducers As a part of daily maintenance inspect the grease around the transducer pin extending from the transducer housing pinhole before starting any experiment Insufficient grease in this area will allow buffer and water to enter the transducer housing and cause damage to the force transducer IMPORTANT NOTES DMT RECOMMENDS THAT THE HIGH VACUUM GREASE SEALING THE TRANSDUCER PINHOLE IS CHECKED AND SEALED AT LEAST ONCE A WEEK ESPECIALLY IF THE MYOGRAPH IS USED FREQUENTLY e DMT TAKES NO RESPONSIBILITIES FOR THE USE OF ANY OTHER KINDS OF HIGH VACUUM GREASE OTHER THAN THE ONE AVAILABLE FROM DMT e DMT TAKES NO RESPONSIBILITIES FOR ANY KIND OF DAMAGE APPLIED TO THE FORCE TRANSDUCERS Linear slides Check the linear slides under the black covers for grease at least once a week In case of insufficient lubrication grease the slides with the Grease for Linear Slides i
6. therefore whether they are the jaws for wires or the pins will need occasional adjustments Changing and adjustment of the supports is performed using the following step by step procedure NOTE THE TRANSDUCERS ARE FRAGILE AND SENSITIVE TO MECHANICAL STRAIN BE VERY CAREFUL WHEN CHANGING OR AD JUSTING THE MOUNTING SUPPORTS Changing the supports Figure 4 1 1 Use the micrometer to separate the supports as far apart as possible 2 Use the small screwdriver provided to gently loosen screw D on the support attached on the transducer side using the small screwdriver Screw D is the screw on the transducer side support closest to the transducer 3 Gently pull the support away from the transducer pin 4 Loosen screw B on the micrometer side with the appropriate fitting allen key 5 Pull the support away Note Number the supports with the chamber number they were removed from using some kind of permanent marker Store the supports in the provided plastic case Numbering the supports will save time when the sup ports are changed again limiting the amount of adjustments needed after each change Course adjusting the jaws for small vessels Figure 4 1 6 Loosen screw A to move the micrometer side jaw toward or away from the micrometer Loosen screw D to move transducer side jaw toward or away from the transducer 8 Loosen screw C to vertically alight the transducer side jaw Screw C is the screw on the transducer side support t
7. FORCE REC OUTPUT RANGE Force1 Rec Range 200 mN Force2 Rec Range 1100 mN Force3 Rec Range 20 mN Force4 Rec Range 1200 mN MEASUREMENT RANGE Range Chamber 1 200 mN Range Chamber 1 800 mN Range Chamber 1 400 mN Range Chamber 1 200 mN MEASUREMENT RANGE Range Chamber 1 200 mN Range Chamber 1 800 mN Range Chamber 1 400 mN Range Chamber 1 200 mN CHAPTER3 21 5 Interface settings The INTERFACE SETTINGS sub menu in SETTINGS has an additional 2 sub menus These 2 additional sub menus are INTERFACE SETTINGS l TEMPERATURE DIFFERENCE ONE Il FACTORY DIAGNOSTICS DIFFERENCE FACTORY DIAGNOSTICS TEMPERATURE DIFFERENCE The TEMPERATURE DIFFERENCE function allows the user to finetune TEMS COEPI GAME the temperature setpoint of the system Although the temperature set Chamber 1 1 2 C SELECT point for the system can be set in the HEAT MENU the actual tempera ture for the system may not heat to the exact defined setpoint There Chamber 2 1 1 C fore the user can adjust the temperature of each chamber individually Chamber 3 1 2 C to fine tune the temperature setting so that EXACT temperatures can be achieved for any particular chamber This is referred to as a tem Chamber 4 1 4 C SELECT perature offset TEMP OFFSET ON CHAMBER The SELECT and functions are the same in this menu as previ ously described for the VALVE DELAY menu Pressing ENTER w
8. Illustrations of properly aligned pins depicted on the far left and incorrectly aligned pins depicted in the middle and far right CHAPTER4 25 4 2 Calibration of the force transducer As a part of the general maintenance of the myograph DMT recommends that the myograph is weight calibrated at least once a month The myograph should also be weight calibrated every time the interface has been moved Although lab benches are all Supposedly perfectly horizontal small differences in lab bench pitch can affect the calibration of the system The myograph also should be calibrated if the system has been idle for longer than a month A step by step procedure is included in the FORCE CALIBRATION sub menu under SETTINGS as explained in Chapter 3 See Appendix 3 for Principles of the Weight Calibration Force transducer calibration procedure This section contains step by step instructions to calibrate the force transducer and should be used in conjunction with the steps described in Chapter 3 FORCE CALIBRATION sub menu under SETTINGS NOTE EVERYTIME A FORCE CALIBRATION IS PERFORMED THE MEASUREMENT RANGE IS SET TO DEFAULT 200 mN SET MEASUREMENT RANGE AFTER THE FORCE CALIBRATION 1 Move the jaws pins apart If calibrating the transducer with the jaws in place make sure a wire is mounted on the transduc er side jaw If pins are being used the wire does not have to be put in place Fill the chamber with distilled water or buffer Use the same volume
9. P 3 5 SN ae ecco ae ee serve 6 7 APD OVALS TT MA 7 OF COMTOMIAILY ET TL MUR 8 POLE this manu AN Nt 9 Unpacking the myograph SVSEBITI 10 Chapter 1 System 11 ABS MATE aCe FRONC Nel TETTE TOT 11 1 2 Interface Rear Panel ETE Oe 11 UN M t wire Ay OCA saisie a R A aA EOE A a a a 12 CGhapter 2 Setting ente C I 13 2 1 The Complete Myograph 620M viet spain CE nnt o denn d EX M RC 13 2 2 13 2 3 1he first force transducer Call 6 21 mtem 14 Chapter 3 The Interface Menus 15 Chapter 4 The Multi Wire Myograph 23 4 1 Changing and adjusting the mounting SUD DOM nir Duc QOO Cue 23 4 2 Galibrauon OF the ia ore a a ER DD or ND a 26 NE TOCE a SN SU cc
10. The mounting procedure was refined in 2 ways 1 both ends of each mounting wire were secured under tension without any direct manipulation of the vessel and 2 segments of small vessels could not be atraumatically mounted as ring preparations in a myograph for recording of highly isometric force measurements During the late 1970s some improvements were made to the myograph and in 1981 a new dual myograph that allowed simul taneous testing of two vessels was introduced In parallel the technique became widely acknowledged resulting in a growing interest in the myograph systems In 1986 the growing demand resulted in the foundation of the private company J P Trading with the purpose of making the myograph systems commercially available worldwide At the same time J P Trading initiated a comprehensive improvement programme for the existing myograph systems as well as a development programme of new myo graph systems in close co operation with Professor M J Mulvany and The University of Aarhus During the late 1980s and through the 1990s several improvements were applied to the myograph systems such as a new mechanical design a more robust transducer and a new electronic system New systems also were introduced such as the automatic dual myograph 510A the multi myograph 610M and the confocal myograph 120CW In 2000 J P Trading changed its company structure and became known as DMT Danish Myo Technology A S Today DMT is one of th
11. recorded in the data acquisition software will not record more than 20 mN and will appear as a flat line trace at 20 mN even though the force readings on the interface may exceed 20 mN Therefore change the FORCE REC OUT settings to an appropriate setting so as to capture any maximal response from the vessel of interest This value should not exceed the settings for the transducer range which is defined by the sub menu MEASUREMENT RANGE and is explained in the next section The SELECT and functions are the same in this menu as previously described for the VALVE DELAY menu Pressing ENTER will store the numbers in memory for future experiments Anytime this function is changed a new weight calibration on the trans ducers should be performed entering the new voltage values into the data acquisition system being used 4 Measurement Range The MEASUREMENT RANGE sub menu in SETTINGS determines the maxi mum force capacity of the transducer The factory setting is 200 mN but the transducer capacity can be changed to 400 mN 800 mN or a maximum of 1600 mN of force detection depending on the size of the vessel used The SELECT and ALL functions are the same in this menu as previously described for the VALVE DELAY menu Pressing ENTER will store the numbers in memory for future experiments Force1 Rec Range 200 mN Force2 Rec Range 1100 mN Force3 Rec Range 20 mN Force4 Rec Range 1200 mN
12. that will be used during the experiments 2 Setup the calibration kit bridge and balance on one of the myograph chambers as illustrated in Figure 4 6 Also place the weight on one of the chambers Turn the heat on as discussed in Chapter 3 The system takes about 20 to 30 minutes to reach 37 C Obviously lower temperatures take less time and higher temperatures take more time to reach Make sure ad equate time is allowed so that calibration can be performed at the temperature at which the experiments will be performed Placing the calibration kit and weight on the chamber allows them to warm up to the experimental target temperature No need to bubble the chambers while waiting for the system to heat up Figure 4 6 Weight calibration kit shown in place on a single myograph chamber 26 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL 3 When the system reaches target temperature adjust the calibration kit so that the tip of the transducer arm is as close to the wire if jaws are being used or pin on the transducer side as possible without touching as illustrated in Figure 4 9 One way to do this is to use the following technique Start with the calibration kit in place so that the transducer arm of the bridge with the pans is not touching any part of the jaw or wire if the jaws are being used or not touching any part of the pins Go to the main menu displaying the forces and zero the channel being calibrated so the force reads zero Slowly an
13. CE TO ZERO menu simply touch the screen to access a menu When a setting needs to be changed the setting can be changed by pressing the SELECT icon on the touch screen corresponding to the desired channel to be changed Force chamber 2 0 26 mN SELECT Force chamber 1 2 36 mN SELECT Force chamber 3 1 06 mN SELECT Force chamber 4 0 76 mN SELECT SELECT The line to be modified will turn blue indicating that the interface is waiting for input When ALL is chosen all lines corresponding to all 4 channels SET FORCE TO ZERO will turn blue Changing the numeric value for the chosen parameter can be Force chamber 1 2 36 mN SELECT done by touching the up or down arrow keys Force chamber 2 0 26 mN SELECT Force chamber 3 1 06 mN SELECT Force chamber 4 0 76 mN SELECT Once the desired setting has been chosen pressing ENTER will lock the selection and be stored in memory Pressing the white X in the red box will exit that menu and take you automatically to the Actual Force Display MEASUREMENT RANGE Range Chamber 1 200 mN SELECT Range Chamber 1 800 mN SELECT Range Chamber 1 400 mN SELECT A Range Chamber 1 200 mN SELECT CHAPTER3 15 Power Up Screen After turning on the 620M Interface an Introduction screen appears The system is auto calibrating the A D converters while this screen is displayed After a few seconds the ACTUAL FORCE display will appear A
14. FS Chamber cover for field stimulation Plastic mounting jaws for field stimulation Combined pulse amp train generator CS200 32 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL NOTES NOTES 33 DMT A S Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Denmark Tel 45 87 41 11 00 Fax 45 87 41 11 01 www dmt dk sales dmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554 www dmt asia com sales dmt asia com support dmt asia com DMT Asia China office Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China Tel 56 0 215425 1330 Fax 86 21 5877 0063 www dmt asia com sales dmt asia com support dmt asia com DMT USA Inc 525 Avis Drive Suite 10 Ann Arbor MI 48108 USA Tel 1 770 612 8014 Fax 1 678 302 7013 www dmt usa com sales dmt usa com support dmt usa com
15. MC Requirements Part 2 6 Particular requirements In vitro diagnostic IVD medical equipment With reference to regulations in the following directives 2006 95 EC 89 336 EEC 8 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL ABOUT THIS MANUAL This manual contains a complete list of procedures that describe how to install maintain and using the Multi Wire Myograph System Model 620M Chapter 1 provides an overview of the construction and basic features of the Interface and the Multi Wire Myograph Unit Chapter 2 describes step by step instructions to set up a complete 620M Wire Myograph System including accessories Chapter 3 is a complete manual to the 620M Interface This chapter describes in detail how to navigate the menus and how to use the special features of the 620M Myograph System Chapter 4 contains procedures describing general and daily maintenance of the myograph unit e g adjustment of supports weight calibration of the force transducer and cleaning instructions Appendix contain additional information such as system specifications ABOUT THIS MANUAL 9 UNPACKING THE MYOGRAPH SYSTEM Take a few minutes to carefully inspect your new Multi Wire Myographs System 620M for damage which may have occurred dur ing handling and shipping If you suspect any kind of damage please contact DMT immediately and the matter will be pursued soon as possible If the packing material appears damaged please retain it until a po
16. R HNO3 BECAUSE THESE ACIDS MAY CAUSE EXTREME DAMAGE TO THE STAINLESS STEEL CHAMBERS AND SUPPORTS DO NOT USE BLEACH TO CLEAN THE CHAMBERS REPEATED USE OF CHLORIN ATED SOLUTIONS SUCH AS BLEACH AND HCL WILL CAUSE DAMAGE TO THE STAINLESS STEEL PARTS OF YOUR MYO GRAPH SYSTEM AVOID USING THEM IF AT ALL POSSIBLE AFTER CLEANING ALWAYS CHECK THAT THE GREASE AROUND THE TRANSDUCER PIN IS SUFFICIENT TO KEEP THE BUFFER AND WATER FROM ENTERING THE TRANSDUCER HOUSING 30 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL If red or brown discolorations appear on the chamber sides or on the supports the following cleaning procedure will work in most cases f Incubate the myograph chamber and supports for 30 minutes with 2mM T 1210 Tetrakis 2 pyridylmethyl ethylenediamine solution dissolved in double distilled water 8 Use a cotton tip applicator to mechanically clean all the affected surfaces during the last 15 minutes of the incubation period 9 Wash the myograph chamber and supports several times with double distilled water 10 Incubate the myograph chamber with 96 ethanol for 10 minutes while continuing the mechanical cleaning with a cotton tip applicator 11 Remove the ethanol solution and wash a few times with double distilled water Incubate the myograph chamber and sup ports with an 896 acetic acid solution for 10 minutes and continue the mechanical cleaning with a swab stick 12 Wash the myograph chamber and supports several times w
17. SEE NESTY PowerLab data acquisition system i 34 optional 1 1 1 1 1 1 1 1 1 1 1 1 1 I 1 1 I 1 1 1 1 1 1 1 1 I 1 1 1 1 I 1 I I 1 1 1 1 I ai Connection to oxygen jJ I a supply I 1 Arr Pit eno _ BNC Cables 1 Vacuum pump optional Suction bottle optional Figure 2 1 The complete Multi Wire Myograph System Model 620M 2 2 Setting up step by step This chapter contains a complete step by step description of how to set up a complete myograph 620M system as illustrated in Figure 2 1 above 1 Interface PC Connection Data acquisition is possible either by connecting the Interface directly to a PC or through a PowerLab data acquisition and analysis system optional l Direct PC Connection Connect the Interface to one of the COM ports on the PC using a serial cable cable not included PowerLab Optional Connect the Interface to the PowerLab unit using BNC cables Connect Rec 1 on the Interface to Input 1 on the Power Lab Rec 2 to Input 2 etc Connect the PowerLab unit to one of the USB ports on the PC using the USB cable delivered with the PowerLab system Oxygen Supply Connect the gas supply 95 05 5 CO or 2196 5 CO balance 5 with tubing running from the gas supply to the gas i
18. TE EVERYTIME A FORCE CALIBRATION IS PERFORMED THE MEAS UREMENT RANGE IS SET TO DEFAULT 200 mN SET MEASUREMENT RANGE AFTER THE FORCE CALIBRATION Entering the FORCE CALIBRATION sub menu begins the transducer calibra tion procedure Begin the calibration procedure by pressing FORCE CALI BRATION to enter the sub menu The sub menu will list all 4 chambers for calibration To begin the calibration press SELECT for the chamber which calibration will be performed on The text for the chamber to be calibrated will turn blue Pressing ENTER will enter the 6 step procedure for calibrating the force transducer on the desired chamber The calibration procedure is listed in 6 individual steps and needs to be per formed for each channel or transducer when calibrating the system Step 1 involves setting up the chamber for calibration Make sure the chamber contains the pins or jaws depending on the type of vessel being studied If jaws are being used for smaller vessels a wire needs to be strung on the transducer side jaw for the calibration Fill the chamber with double distilled water for the volume to be used experimentally Press NEXT STEP Step 2 involves setting up the calibration kit appropriately for the actual weight calibration Verify that the transducer arm pin does not touch the mounting wire on the jaw or the mounting pin for larger vessels as instruct ed The pin should be as close as possible to the mounting wire or mount in
19. THE APPROPRIATE BUT TON FOR AN ADDITIONAL 3 5 SECONDS AFTER THE INITIAL EMPTYING THIS WILL HELP DRAIN RESIDUAL BUFFER AND SOLUTIONS RETAINED IN THE TUBING AND VALVES e Funnel Temperature probe For drug application Figure 2 2 Suction connection Figure 2 3 Chamber cover 4 Chamber Covers The chamber covers will help maintain the temperature and other buffer conditions gas tension pH fairly constant Holes in the chamber covers serve different purposes and they are illustrated in Figure 2 3 above The slots allow the covers to be placed over the chamber around the support arms and gas vacuum tubes 2 3 The first force transducer calibration Prior to the shipment of the Multi Wire Myograph 620M System has gone through two days of continuous testing including a final force transducer calibration However DMT recommends that a new force transducer calibration is performed before using the myograph system for the first time The force transducer calibration procedure is described in detail in the FORCE CALIBRATION sub menu under SETTINGS as explained in Chapter 3 14 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL CHAPTER 3 THE INTERFACE MENUS Chapter 3 is a complete manual for the 620M Interface The chapter contains a detailed description of how to navigate the touch screen menus and how to use the special features of the 620M myograph Menus on the 620M interface are all accessible by a touch screen To access SET FOR
20. d gently slide the calibration kit forward toward the micrometer so that the transducer arm rests on the wire or pin creating a force read ing on that channel Carefully slide the calibration kit back toward the transducer slowly until the force reads zero or very close to zero At this point as soon as the force reads zero the transducer arm will be properly placed for weight calibration 4 Goto the FORCE CALIBRATION sub menu of the SETTINGS menu on the Interface to begin the actual transducer calibration The process that is described above is reiterated in 6 steps once the FORCE CALIBRATION sub menu is initiated which is described in detail in Chapter 3 Transducer arm Figure 4 7 Illustration of the proper placement for the balance transducer arm for calibration CHAPTER4 27 4 3 Checking the force transducer The myograph force transducer is a strain gauge connected to a Wheatstone bridge The force transducers for each chamber are housed in a separate protective compartment See Figure 4 8 below While the protective cover offers some mechanical protec tion for the force transducers they are still very vulnerable to applied forces exceeding 1 Newton 100 grams or fluid running into the transducer compartment due to insufficient greasing of the transducer pinhole Figure 4 8 Illustration of the proper transducer house If the force readings on the Interface appear unstable or noisy then first check that the chambers are co
21. e chamber for the first cali bration to monitor when the chamber has reached the target temperature Heating will take about 20 to 30 minutes for the chambers and transducers to come to 37 with the chamber covers in place Once the chamber s are heated and have reached the target temperature press NEXT STEP Step 4 is the first step in the actual weight calibration process A 4 digit number will be displayed in blue at the bottom of the screen If nothing has been perturbed during the heating process the zero gram 0 00 mN calibration should be stable as indicated by the 4 digit number and NEXT STEP can be pressed at this time If the 4 digit number is not stable then wait until the number has stopped fluctuating before pressing NEXT STEP Step 5 is the 2 gram weight calibration At this step place the 2 gram weight in the pan closest to the transducer so as to simulate a vessel pulling on the jaw or pin attached to the transducer Remember a 2 gram weight in 90 vector is cut in half and the transducer will only detect 1 gram or 9 81 mN of force The weight placement should cause a positive increase in the 4 digit number Wait at least 10 to 15 seconds for the applied force to sta bilize before pressing NEXT STEP Once the 4 digit number has stabilized press NEXT STEP CHAMBER 1 CALIBRATION Step no oB 5 Turn the heat on Wait until the temperature is stable Temperature set point 37 0
22. e world s leading designers and manufacturers of wire myographs pressure myographs culture myo graphs and organ tissue baths Driven by our global customer base our singular goal is to develop and manufacture first class research equipment within the fields of physiology and pharmacology INTRODUCTION 5 SAFETY The 620M Multi Wire Myograph System has been designed for use only in teaching and research applications It is not intended for clinical or critical life care use and should never be used for these purposes or for the prevention diagnosis curing treat ment or alleviation of disease injury or handicap e Do not open the unit the internal electronics pose a risk of electric shock Do not use this apparatus near water e To reduce the risk of fire or electric shock do not expose this apparatus to rain or moisture Objects filled with liquids should not be placed on the apparatus Do not block any ventilation openings Install in accordance with the manufacturer s instructions Do not install near any heat sources such as radiators heat registers stoves or other equipment or devices that produce heat e Only use attachments and accessories specified by the manufacturer e Unplug this apparatus during lightning storms or when unused for long periods of time e Be advised that different operating voltages require the use of different types of line cord and attachment plugs Check the voltage in your area a
23. ea se tec UU 28 4A Force Iransducer Replacement ORE CRUDUM 29 Ae SIS SISTI E 30 Appendix 1 System specifications ERE E RR S KB ER DN RO E En 32 ji AEAN tes A eee PEA lee eee AE A 33 4 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL INTRODUCTION Until the mid 1970s most of the information about the mechanical morphological and pharmacological properties of vascular smooth muscle were only obtainable from studies on relatively large vessels At that time rat tail arteries were the smallest ves sels to be investigated in detail due to limitations in the available in vitro techniques For example studies measuring the con traction force were routinely performed with only one of the mounting wires secured Futhermore relatively large wires 100 200 were used which precluded the use of small vessels In addition the vessel segment had to be directly manipulated with dissecting instruments causing mechanical trauma Investigations of smaller vessels therefore were limited to in vivo perfusion experiments and histological examination In 1976 Professor M J Mulvany and Professor W Halpern described for the first time a new technique that made it possible to investigate highly isometric responses from vessels with internal diameters as small as 100 um
24. erature are controlled from this menu To turn the heat on or change the preset temperature for the system access the temperature control menu Pressing the HEAT key will enter the menu and allow the user to change the default system temperature as well as turn the heat on or off Pressing DEFAULT will automatically reset the temperature setpoint to 37 C Manually change the temperature by pressing the up or down arrows To turn the heat on touch ON and the ON icon will turn green indicat ing the heat has been turned on The system will heat to the designated temperature setpoint Pressing the white X in the red box will send the user back to the ACTUAL FORCE display Settings Menu The Settings Menu contains several sub menus that can be accessed to change functional aspects of the interface These sub menus include 1 FORCE CALIBRATION 2 VALVE DELAY 3 FORCE REC OUTPUT 4 MEASUREMENT RANGE 5 INTERFACE SETTINGS SET FORCE TO ZERO Force chamber 1 2 36 mN SELECT Force chamber 2 0 26 mN SELECT Force chamber 3 1 06 mN SELECT Force chamber 4 0 76 mN SELECT Temperature setpoint 37 0 C Probe temperature 36 6 C HEAT SET CHAMBER TEMPERATURE Temperature setpoint 37 0 C Probe temperature 36 6 C HEAT SETTINGS FORCE VALVE FORCE REC MEASUREMENT CALIBRATION DELAY OUTPUT RANGE INTERFACE SETTINGS CHAPTER3 17 1 Force calibration NO
25. g pin without touching in order to get the most accurate calibration Press NEXT STEP when the calibration kit has been properly placed 18 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL FORCE CALIBRATION Force calibration chamber 1 SELECT Force calibration chamber 2 SELECT Force calibration chamber 3 SELECT Force calibration chamber 4 SELECT FORCE CALIBRATION Force calibration chamber 1 SELECT Force calibration chamber 2 SELECT Force calibration chamber 3 SELECT Force calibration chamber 4 SELECT CHAMBER 1 CALIBRATION Step 0 89 2 C3 C3 C9 C Follow the Weight calibration procedure in the User Manual Prepare the jaws and chamber for calibration When ready go to next step CHAMBER 1 CALIBRATION Step no OBOLO Place the calibration bridge on the myograph Be careful when placing the bridge The pin must not touch the wire jaw When ready go to next step NEXT STEP Step 3 initiates the heating process for the chambers In order for the cali bration to be accurate the transducers must be heated to the experimen tal temperature to be used to accommodate heat induced expansion of the electronic parts in the transducer Otherwise inaccurate readings and transducer drift may occur introducing large errors into the experiment To start heating press HEAT ON Covering the chambers with the chamber covers will expedite the chamber heating Place the temperature probe into th
26. hat is fur thest away from the transducer Figure 4 1 Myograph unit screws for changing supports and coarse adjustment of the jaws CHAPTER4 23 Fine adjusting the jaws for small vessels Figure 4 2 and Figure 4 3 9 Tightening Screw will move the micrometer side jaw downward and to the left 10 Tightening both screws 111 will move the micrometer side jaw straight down 11 Tightening both screws II and IV will move the micrometer side jaw straight up Micrometer e Figure 4 2 Fine adjustments of the jaws in the myograph chamber Jaws from top view Jaws from side view We Ca w v x x Figure 4 3 Illustrations of properly aligned jaws depicted on the far left and incorrectly aligned jaws depicted in the middle and far right 24 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL Fine adjusting the pins for larger vessels Figure 4 4 and Figure 4 5 12 Loosen screw A to move the micrometer side arm holder sideways 13 Loosen screw B to move the micrometer side pin toward or away from the transducer 14 Loosen screw C to align the transducer side pin horizontally 15 Loosen screws D and E to align the heights of the pins vertically ESTA a AB E DC Figure 4 4 Fine adjustments of the pins in the myograph chamber Pins from top view Pins from side view M o Y Figure 4 5
27. ill store the numbers in memory for future experiments TEMP OFFSET ON CHAMBER X Chamber 1 2 SELECT Chamber 2 SELECT Chamber 3 SELECT Chamber 4 SELECT FACTORY DIAGNOSTICS Entering FACTORY DIAGNOSTICS will display the LOGIN CODE TO DIAG NOSTICS window This window is for trained technicians and used for diagnostics and troubleshooting purposes The general user will not Type login code to get acces have access to this window Entering the proper 5 digit pin number 5 7 5 however will allow the trained technician access Diagnostics panels that will provide information during a malfunction or mechanisms to 5 e change other settings controlled by the onboard computer Code init value 51761 22 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL CHAPTER 4 THE MULTI WIRE MYOGRAPH UNIT This chapter contains a complete explanation of how to adjust calibrate and maintain the myograph 620M system so that the myograph is always performing at peak performance 4 1 Changing and adjusting the mounting supports Each chamber can accommodate mounting supports for either small vessels gt 5Oum or larger segments gt 500 Because the mounting supports can be changed easily experiments can be performed with different vessels of varying internal diameter Continuous use and repeated greasing of the transducer arm holes will cause some misalignment of the mounting supports The mounting supports
28. ith double distilled water 13 Dry the surfaces using absorbent paper i e Kim Wipes or cotton tip applicators IMPORTANT NOTES IN EXCEPTIONAL CASES THE SUPPORTS JAWS OR PINS MAY NEED TO BE REMOVED FROM THE MYOGRAPH CHAMBER AND CLEANED INDIVIDUALLY TO ASSURE PROPER CLEANING OF ALL SUPPORT SURFACES NEVER SOAK THE SUPPORTS IN ANYTHING STRONGER THAN 8 ACETIC ACID FOR EXTENDED PERIODS OF TIME I E SEVERAL HOURS OR OVERNIGHT CHAPTER4 31 APPENDIX 1 SYSTEM SPECIFICATIONS Technical specifications Vessel size Chamber Chamber material Chamber volume Chamber suction Chamber cover Chamber gassing Force range Force resolution Micropositioners Weight calibration Heating Temp range Temp resolution Temp probe Output reading Analogue output Voltage Ambient temp 260 um 450 um up to 10 mm Four individual chambers Acid resistant stainless steel Max 8 ml Manual or automatic time controlled user defined Supplied with connections for gassing Individually controlled per chamber by needle valves User selectable at 200 400 800 1600 mN 0 1 mN Manually operated precision micrometer Semi Automatic Built into chamber independent of superfusion Ambient temp 45 C 0 1 C External Force mN Independently filtered 4 channel output at 2 5V full scale 100 to 240 VAC auto 50 60 Hz via external power supply 15 30 C Optional accessories Automatic Buffer Filler System 625
29. mined by monitoring the interference while turning the equipment off and on the user is encouraged to correct the interference by one or more of the following measures e Reorient or relocate the receiving antenna e Increase the separation between the equipment and receiver e Connect the equipment into an outlet on a circuit different to that which the receiver is connected to e Consult the dealer or an experienced radio TV technician for help APPROVALS Complies with the EMC standards EN 61326 2 6 2005 EMC 89 336 EEC EN 61000 3 2 Certified with the safety standards EN 61010 1 2001 Directive 2006 95 EC EN 61010 1 Corr 1 2003 EN 61010 1 Corr 1 2003 EN 61010 2 101 2003 EMC EMI 7 CERTIFICATE OF CONFORMITY DMT A S Skejbyparken 152 8200 Aarhus N Denmark hereby declares its responsibility that the following product Multi Wire Myograph System Model 620M is covered by this certificate and marked with CE label conforms with the following standards EN 61010 1 2001 Safety requirements for electrical equipment for measurement con EN 61010 1 Corr 1 2003 trol and laboratory use EN 61010 1 Corr 1 2003 Part 1 General requirements EN 61010 2 101 2003 Safety requirements for electrical equipment for measurement con trol and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD medical equipment Electrical equipment for measurement control and laboratory use EN 61326 2 6 2005 E
30. ncluded with your system Cleaning the myograph DMT strongly recommends that the myograph chambers and surrounding areas are cleaned after each experiment At the end of each experiment use the following procedure to clean the myograph chambers and supports 1 Fill the myograph chamber to the edge with an 8 acetic acid solution and allow it to work for a few minutes to dissolve calcium deposits and other salt build up Use a cotton tipped applicator to mechanically clean all chamber surfaces 2 Remove the acetic acid and wash the myograph chamber and supports several times with double distilled water 3 If any kind of hydrophobic reagents have been used which might be difficult to remove using steps 1 and 2 then try incu bating the chamber and supports with 96 ethanol or a weak detergent solution i e 0 1 triton 100 4 To remove more resistant or toxic chemicals incubate the myograph chamber and supports with 1M HCI for up to 1 hour In exceptional cases incubate the chamber and supports with no stronger than a HNO3 solution for about 15 minutes 5 Wash the myograph chamber and supports several times with double distilled water 6 If acids such as 1M and are used to clean the chambers make sure ALL surfaces are thoroughly dried after copious washes with double distilled water Any residual acid will cause corrosion of the stainless steel jaws and pins IMPORTANT NOTES BE VERY CAREFUL USING HCL O
31. nd use the correct type See the table below Voltage Line plug according to standard 110 125 V UL81 and CSA C22 2 No 42 220 230 V CEE 7 page VII SR section 107 2 D1 IEC 83 page C4 240 V BS 1363 of 1984 Specification for 13A fused plugs and switched and unswitched socket outlets Protect the power cord from being walked on or pinched particularly at power outlets and the point where they connect to the apparatus Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way such as the power supply cord or plug is damaged liquid has spilled onto or objects have fallen into the apparatus the apparatus has been exposed to rain or moisture does not operate normally or has been dropped 6 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL EMC EMI This equipment has been tested and complies with the limits for a Class B Digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference in residential installations This equip ment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be deter
32. nlet on the back of the Interface Oxygen is supplied to the chambers by tubing attached to the stainless steel vacuum pipe The oxygen and vacuum tubing need to be inserted into the chamber in order to aerate the heated buffer Needle valves on the back of the interface can be adjusted to regulate the amount of bubbling that occurs Turning the regulator clockwise increases the bubbling while turning it counter clockwise decreases the bubbling Each regulator has a lock device attached that can be used when the desired bubbling is achieved See Figure 2 2 on next page CHAPTER2 13 NOTE THE NEEDLE VALVES NEED TO BE GREASED USING THE GREASE FOR THE LINEAR SLIDES AND TURNED AT REGULAR INTERVALS TO PREVENT THEM FROM STICKING OR PERMANENTLY FREEZING 3 Vacuum Connection The system has a built in manifold with separate valves that allows each chamber to be drained individually After connect ing the vacuum source at the back of the Interface the vacuum pipes need to be inserted into the chambers in order for this feature to work properly The pipes are inserted into the chamber by gently pulling up on the curved part of the pipe turning it 90 counter clockwise and gently lowering it into the chamber A chamber can then be emptied by pressing the corresponding numbered button Pressing the all button will empty all the chambers at the same time see Figure 2 2 below NOTE WHEN DRAINING THE CHAMBERS USING THE AUTOMATIC VACUUM FUNCTION PRESS
33. nnected properly to the Interface and that the chambers are plugged all the way into the interface If the force reading s are still unstable or noisy then perform a new calibration of the force transducer as described in Chapter 3 and Chapter 4 2 During the new calibration monitor the relative force reading values in the FORCE CALIBRATION sub menu on the Interface Steps 4 and 5 of the calibration procedure The normal operating values for the force transducer during calibration should be between 3000 and 3500 e fthe value is a single digit or a three digit number the force transducer is broken and needs to be replaced e lf the value is less than 2000 or greater than 4500 the force transducer is broken and needs to be replaced e If the message OFF is displayed on the main page of the Interface even though the chamber is plugged in at the rear of the interface the force transducer is broken and needs to be replaced In addition if the force reading s appear yellow in color cannot be reset to zero AND the transducer cannot be recalibrated the force transducer is broken and needs to be replaced If any other problems related to the force transducer are encountered please contact DMT for advice or further instructions 28 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL 4 4 Force Transducer Replacement If the force transducer breaks and needs to be replaced follow this step by step replacement procedure carefully
34. ssible claim has been settled We recommend that you store the packing material for any possible future transport of the Wire Myograph System In case of transport and the original packing material is unavailable please contact DMT Sales Department for advice and packing instruc tions After unpacking your new Multi Wire Myograph System please use the following list to check that the system is complete e 1 interface unit chamber units with mounted stainless steel jaws e 4 set mounting support pins 200um e chamber covers e 1 external temperature probe e 1 power cord e 1 calibration kit including bridge balance and 2 gram weight e 4 plastic funnels 1rollof 40 um stainless steel wire e tube of high vacuum grease e 1tube of grease for linear slides e 5 spare screws for mounting of jaws e 3Allen keys e 1 small screwdriver e 1 CD with user manuals for Wire Myograph Systems e 1 CD with the manual Procedures for investigation of small vessels using small vessel myograph by Professor M J Mulvany Department of Pharmacology Aarhus University Denmark and the video Dissection and mounting of small vessels in wire myographs The shape of the AC plug varies by country be sure that the plug fits the outlets for your location 10 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL CHAPTER 1 SYSTEM OVERVIEW 1 1 Interface Front Panel
35. t any given time if the force applied on any channel is out of range the force reading for the overloaded channel will turn yellow as a warning Three menus are accessible from the default Actual Force screen or dis play These menus are Zero Heat and Settings 16 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL DMT620 MYOGRAPH Multi Myograph System Model DMT 620 Software Revision 03 00 09 Date Nov 27 2009 ACTUAL FORCE Force chamber 1 Force chamber 2 Force chamber 3 Force chamber 4 Probe temperature ACTUAL FORCE Force chamber 1 234 36 mN Force chamber 2 0 26 mN Force chamber 3 1 06 mN Force chamber 4 0 76 mN Probe temperature 37 0 C ACTUAL FORCE Force chamber 1 2 36 mN Force chamber 2 Force chamber 3 Force chamber 4 Probe temperature 37 0 C b unm 9 g T T Zero Menu This menu is used to zero the output of the transducers When using a data acquisition program like LabChart by AD Instruments using this feature will reset the baseline of the chart traces without affecting the calibrations or physically changing any pre load tensions placed on the mounted ves sels The channels can be changed individually by pressing SELECT or all at once by pressing ALL Pressing ENTER will execute the zero function and return the user to the ACTUAL FORCE display Heat Menu The heating unit and temp
36. used to modify the length of time the vacuum valves stay open after the valves have been acti vated with the push buttons on the front panel of the interface Pressing ALL will cause all the lines to turn blue meaning all chambers can be modified at the same time Again the up and down arrow keys can be used to modify the length of time the vacuum valves stay open Pressing ENTER after modifying the value s for valve delay will lock in the number s and be retained in memory every time the system is turned on 20 WIRE MYOGRAPH SYSTEM MODEL 620M USER MANUAL CHAMBER 1 CALIBRATION Step no 3 65 689 The transducer is now calibrated Force read out should be 9 81 mN 0 1 mN If OK go to next step Otherwise repeat the calibration Force Chamber 1 9 81 mN EMPTY VALVES DELAY Chamber 1 Chamber 2 Chamber 3 Chamber 4 EMPTY VALVES DELAY Chamber 1 Chamber 2 Chamber 3 Chamber 4 EMPTY VALVES DELAY Chamber 1 Chamber 2 Chamber 3 Chamber 4 NEXT STEP 3 Force Rec out The FORCE RECORDING OUTPUT or FORCE REC OUT sub menu determines the upper limit for force sent from the BNC analogue output connectors This will only affect the data collected from the interface to a data acquisi tion system such as AD Instruments PowerLab and LabChart software The factory default setting for FORCE REC OUT is 20 mN meaning that if the force of the mounted vessel exceeds 20 mN the force
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