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Agilent 1260 Infinity Binary Pump User Manual

1. p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low z NC 4 Blue Shut down Low e oa NC 5 Pink Not Ls connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1260 Infinity Binary Pump User Manual Identifying Cables 12 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low o 3 3 Gray Start Low z NC 4 Blue Shut down Low e o NC 5 Pink Not connected NC 6 Yellow Power on High E 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 A D Pin Agilent Signal Name Active module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7
2. module an 1 1 BCD 5 20 2 2 BCD7 80 Se 15 oO Z 3 3 BCD 6 40 oO So 4 4 BCD 4 10 s je 2 5 5 BCDO 1 fie e9 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 210 1260 Infinity Binary Pump User Manual CAN LAN Cables Identifying Cables 12 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 1260 Infinity Binary Pump User Manual Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection 211 12 Identifying Cables External Contact Cables c50 o o ol 10 6 15 41 One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent Module Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected
3. Pump housing Piston housing 2 Remove the support rings from the piston housing and lift the housing away from the pistons Piston housing Piston 160 1260 Infinity Binary Pump User Manual Maintenance 10 3 Check the piston surface and remove any deposits or layers Most suitable is polishing of the piston rod with toothpaste Replace the piston if scratched or if dents are visible Piston surface The best way to inspect a piston is to hold it up and watch e g a light bulb through the piston rod The transparent sapphire acts as a very strong magnifier and even smallest surface abnormalities become visible 4 Using the steel side of the insert tool carefully remove the seal from the pump housing Remove wear retainers if still present Insert tool Piston seal Pump housing es a 5 Using the plastic side of the insert tool insert the new seals into the pump head and press them firmly in position Insert tool Piston seals 6 Place a seal wash gasket in the recess of the support ring Put the seal holder on top of the gasket Seal holder Gasket Support ring 1260 Infinity Binary Pump User Manual 161 10 Maintenance 7 Reassemble the pump head assembly Note the correct 8 Insert the pistons and carefully press them into the seals position of the pin on the support ring Piston a Pump housing Support rings t Pist
4. 1260 Infinity Binary Pump User Manual Valve Test Test Functions and Calibration 8 Description The Valve Test provides a fast and accurate way to verify proper hydraulic operation of the binary pump Problems that are related to defective valves seals or pistons can be diagnosed and usually the defective part is identified If a minimum firmware of A 06 50 is installed on this module and a minimum Lab Advisor revision of B 02 03 is available please run the improved Leak Rate Test instead see Leak Rate Test on page 137 Step 1 The system is setup with water on both channels and a restriction capillary is attached to the outlet of the pump Pump head A is delivering at 1 mL min The pressure signal is monitored and overlaid with the piston movement plot The pressure pattern and the slope of the pressure signal are evaluated for the delivery strokes of both pistons Step 2 The procedure from step 1 is repeated on pump head B Step 3 The data from step 1 and 2 are evaluated In case test failed a conclusion about the defective part is made 1260 Infinity Binary Pump User Manual 131 8 Test Functions and Calibration Running the Valve Test When Tools required Parts required Preparations Running the test from the Agilent Lab Advisor For FW revisions lt A 06 50 The test should be used to prove proper operation of the binary pump after repairs or when the pressure test see System Pres
5. 212 1260 Infinity Binary Pump User Manual RS 232 Cable Kit p n G1530 60600 RS232 61601 5181 1561 1260 Infinity Binary Pump User Manual Identifying Cables 12 Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m 213 12 Identifying Cables Agilent 1200 Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control Module 214 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual 13 Hardware Information Firmware Description 216 Electrical Connections 219 Rear view of the module 220 Interfaces 221 Overview Interfaces 224 Setting the 8 bit Configuration Switch without On board LAN 228 Communication Settings for RS 232C 229 Special Settings 231 Optional Interface Boards 232 The Solvent Selection Valve SSV 236 Early Maintenance Feedback 237 Instrument Layout 238 This chapter provides detailed technical information about your binary pump rae Agilent Technologies 215 13 Hardware Information Firmware Description The firmware of the instrument consist
6. Agilent Technologies Notices Agilent Technologies Inc 2005 2008 2010 2013 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61312 90015 Rev B Edition 11 2013 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user hav
7. p n G1312B 61311 60003 2x G1369C 61312 67500 61312 90303 G4201 68707 G4202 68707 64203 68708 827975 902 959961 902 699975 302 699975 902 5067 4770 G4800 64500 M8500A Description Agilent 1260 Infinity Binary Pump optionally with active seal wash and or solvent selection valve Bottle head assembly OPTIONAL Interface board LAN OPTIONAL Calibration capillary assembly Agilent 1260 Infinity Binary LC System User Guide not orderable HPLC Starter Kit incl 0 17 mm i d cap OPTIONAL HPLC Starter Kit incl 0 12 mm i d cap OPTIONAL HPLC System Tool Kit OPTIONAL Column SB C18 4 6x50 mm 1 8 um 600 bar OPTIONAL Column Eclipse Plus C18 4 6 x 100 mm 3 5 pm OPTIONAL Column Poroshell 120 EC C18 3 0 x 50 mm 2 7 pm OPTIONAL Column Poroshell 120 EC C18 4 6 x 50 mm 2 7 pm OPTIONAL Solvent Cabinet Kit OPTIONAL Agilent 1200 Infinity Series User Documentation DVD OPTIONAL not orderable OPTIONAL Lab Advisor incl license OPTIONAL Power cord Items identified as optional are additional accessories They are not included in the standard scope of delivery Items identified as not orderable can be downloaded from the Agilent website http www agilent com 1260 Infinity Binary Pump User Manual 31 3 Installing the Pump Accessory Kit The Accessory Kit G1311 68755 contains the following items p n Description 5062 2461 Waste tube 5 m reorder pack 5063 6527 Tubing as
8. 3 Installing the Pump Regular Priming When When the pumping system has been turned off for a certain time for example overnight air will rediffuse into the solvent channel between the vacuum degasser and the pump If solvents containing volatile components are left in the degasser without flow for a prolonged period there will be a slight loss of the volatile components Preparations Switch the system on The purge tool of the Lab Advisor can be used for automatically purging the pump 1 Open the purge valve of your pump by turning it counterclockwise and set the flow rate to 5 mL min Flush the vacuum degasser and all tubes with at least 10 mL of solvent Repeat step 1 and 2 for the other channel s of the pump Set the required composition and flow rate for your application and close the purge valve 5 Pump for approximately 10 minutes before starting your application 56 1260 Infinity Binary Pump User Manual Installing the Pump 3 Changing Solvents When Parts required Preparations CAUTION When the solvent of a channel is to be replaced by another solvent that is not compatible solvents are immiscible or one solvent contains a buffer it is necessary to follow the procedure below to prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system p n Description 1 Purging solvent s see Table 3 on page 58 1 5022 2184 Union ZDV Remove the column and replace it by
9. Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 244 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1260 Infinity Binary Pump User Manual Appendix 14 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 1260 Infinity Binary Pump User Manual 245 14 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 246 1260 Infinity Binary Pump User Manual Appendix 14 Agilent Technologies on the Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent
10. M main assemblies overview 152 maintenance feedback 237 pump head with seal wash 153 pump head without seal wash 153 replacing firmware 181 181 message ignition without cover remote timeout 104 108 108 minimum flow rate 61 missing parts 31 missing pressure reading 112 mixer 11 whentoremove 84 motor drive power 116 non operating altitude 25 non operating temperature 25 0 operating Altitude 25 operating temperature 25 250 153 173 192 128 132 134 136 outlet valve overpressure error P packaging damaged 30 parts identification cables 201 parts active inlet valve 194 bottle head assembly 197 damaged 31 hydraulic path with solvent selection valve 184 hydraulic path without solvent selection valve 186 missing 31 outlet valve 192 system tool kit 196 performance specifications 26 pH range 26 physical specifications 25 piston 13 power consideration 22 power consumption 25 power cords 23 power supply indicator 94 power switch 46 pressure above upper limit 109 pressure below lower limit 110 pressure pulsation 17 88 pressurerange 82 pressure ripple 133 pressure signal missing 110 pressure test 92 results 132 pressure operating range 26 pulsation 26 priming with a pump 56 PTFE frit 153 pulsation pressure 17 ump elasticity calibration 93 136 ump head assembly 153 ump head 153 153 p p pump head missing 119 p pump seals alternative material 82 for normal
11. 1260 Infinity Binary Pump User Manual Test Functions and Calibration 8 curve segment corresponds to the flow and leak rate at that time and the leak rate is measured Subsequently piston 2 is moved to its rear position then piston 2 delivers and the measurement is done as described for piston 1 For a binary pump G1312B C K1312B the test is run for both pump heads for channels A and B Running the Test Parts required p n Description 01080 83202 Blank nut Running the test from the Agilent Lab Advisor 1 Select the Leak Rate Test from the Test Selection menu 2 Start the test and follow the instructions Make sure to release the pressure by slowly opening the purge valve when the test has finished 1260 Infinity Binary Pump User Manual 139 8 Test Functions and Calibration Leak Rate Test Evaluating the Results Results of the leak rate test are the leak rates measured for pistons 1 and 2 as described for the test principle If any of the leak rates exceeds 3 uL min the test will fail Damper ES Pump chamber 1 o Pump chamber 2 Pe Purge valve Outlet valve gt To column Inlet valve To waste z Seal Piston 1 C Piston 2 140 1260 Infinity Binary Pump User Manual Test Functions and Calibration 8 Potential Causes of Leak Rate Test Failure For binary pumps secondary pump chambers are connected A leak observed in any of these chambers may be caused by the other secondary pump chamber
12. e LAN connector as interface to the control software RS 232C as interface to a computer REMOTE connector as interface to other Agilent products e Analog output connector s for signal output 1260 Infinity Binary Pump User Manual 223 13 Hardware Information 224 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the
13. 1260 Infinity Binary Pump User Manual 77 4 Using the Pump Prevent Blocking of Solvent Filters 78 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual 5 Optimizing Performance When to Use a Vacuum Degasser 80 Operational Hints for the Vacuum Degasser 80 When to Use the Active Seal Wash Option 81 When to Use Alternative Seals 82 When to Use the Low Volume Mixer 83 When to Remove Damper and Mixer 84 Convert the Binary Pump to Low Delay Volume Mode 85 How to Optimize the Compressibility Compensation Setting 87 Solvent Compressibility Calibration 87 Optimization of Legacy Compressibility Settings 88 This chapter gives information on how to optimize the performance of the Binary Pump under special operational conditions ee Agilent Technologies 79 5 Optimizing Performance When to Use a Vacuum Degasser 80 A degasser removes air which is dissolved in any solvent When solvents are heated or mixed with other solvents air can leave the solvent and form small bubbles Over time these bubbles accumulate and can cause pressure fluctuations which may finally result in retention time shifts The G4225A High performance Degasser and the G1322A Vacuum Degasser are external degassers that are designed to be used with Agilent 1260 Infinity binary pumps or 1100 1200 Series pumps 1260 Infinity quaternary pumps 1290 Infinity pumps and 1120 1220 Infinity pumps have built in degassers A degasser is need
14. A2 B1 and B2 of the solvent selection valve Make sure to use the brown bottle for the aqueous solvent usually channel A1 6 Label the tubes accordingly using the supplied stickers and fix the tubes in the clips of solvent cabinet and binary pump 7 Hold the waste tubing with a piece of sandpaper and push it onto the purge valve outlet Place the end into your waste system 8 If the pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the waste tube to the waste outlet of the pump leak handling system 9 Connect the pump outlet capillary pump to injection device to the outlet of the purge valve 1260 Infinity Binary Pump User Manual 49 3 Installing the Pump Flow Connections with Solvent Selection Valve 10 Purge your system prior to the first use see Initial Priming on page 54 Bottle head assembly Solvent cabinet Degasser Ss Channel Al aw aoe Channel A2 Static mixer W Cm Pressure sensor age an DH Purge valve ie TEER Pump head A a i Rg e aml Pump head B ER se fap See Im Active inlet valve B T SE ee rt Active inlet valve A H Jh ren Waste tubing Outlet capillary to autosampler Channel B2 Channel B1 Solvent selection valve Figure 17 Binary Pump with Solvent Selection Valve 50 1260 Infinity Binary Pump User Manual Installing the Pump 3 Flow C
15. Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes K1314F Clinical Ed G4212A B DAD 2 No Yes Yes 1 Yes K4212B DAD Clinical Ed G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 2 Yes K1321B FLD Clinical Ed G1321C FLD G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A C TCC 2 No No Yes No Yes K1316C TCC Clinical Ed G1322A DEG No No No No No Yes AUX K1322A DEG Clinical Ed G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes K4225A DEG Clinical Ed 222 1260 Infinity Binary Pump User Manual Hardware Information 13 Table 14 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4227A Flex Cube 2 No No No No No CAN DC OUT for CAN slaves 1 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED K1330B 1 Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with ad ditional G1369C LAN Card The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN CAN connectors as interface to other modules
16. Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required 102 1260 Infinity Binary Pump User Manual Shutdown Error ID 0063 Error Information 7 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing 1260 Infinity Binary Pump User Manual Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition Check the vacuum degasser for an error cond
17. The compensation algorithm is so powerful that the damper and mixer can be removed from the pump flow path at low flow rate while the pressure ripple and composition ripple remain at low levels For method compatibility reasons the legacy compressibility compensation is still available Solvent Compressibility Calibration Unlisted or premixed solvents can be calibrated with the Solvent Compressibility Calibration function For a detailed description see Binary Pump Solvent Compressibility Calibration on page 133 1260 Infinity Binary Pump User Manual 87 5 88 Optimizing Performance Optimization of Legacy Compressibility Settings The compressibility compensation default settings are 50 x 10 bar best for most aqueous solutions for pump head A and 115 x 10 6 bar to suit organic solvents for pump head B The settings represent average values for aqueous solvents A side and organic solvents B side Therefore it is always recommended to use the aqueous solvent on the A side of the pump and the organic solvent on the B side Under normal conditions the default settings reduce the pressure pulsation to below 2 of system pressure which is sufficient for most applications If the compressibility values for the solvents used differ from the default settings it is recommended to change the compressibility values accordingly Compressibility settings can be optimized by using the values for various solvents described
18. e for boot test modes DIPS 1 2 must be UP plus required mode For normal operation use the default best settings Switch settings provide configuration parameters for serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN 1260 Infinity Binary Pump User Manual Hardware Information 13 The following tables represent the configuration switch settings for the modules without on board LAN only Table 17 8 bit Configuration Switch without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 RSVD SYS RSVD RSVD FC The LAN settings are done on the LAN Interface Card G1369B C Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 18 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 Rs 232c 0 1 Baudrate Data Bits Parity Use the following tables for selecti
19. 5 m for seal wash option Capillary ST 0 17 mm x 400 mm S S Capillary ST 0 17 mm x 700 mm S S Waste tube 5 m reorder pack 1260 Infinity Binary Pump User Manual Parts and Materials for Maintenance 11 Hydraulic Path with Solvent Selection Valve Figure 25 Hydraulic Path with Solvent Selection Valve 1260 Infinity Binary Pump User Manual 185 11 Parts and Materials for Maintenance Hydraulic Path without Solvent Selection Valve 186 Item 10 12 13 14 15 16 17 18 p n G1322 67300 0100 1847 G1312 60025 G1312 60056 G1312 60067 G1312 87300 G1312 67302 G1312 87301 G1312 87305 G1312 87330 G1312 87306 G1312 04100 G1312 60061 G1312 87303 G1312 87304 5062 2461 5042 8507 5065 9978 Description Kit of 4 solvent tubes including labels for connection degasser to SSV PEEK adapter 1 4 28 to 10 32 Adapter AIV to solvent inlet tubes Active inlet valve body without cartridge Pump Head 1200 SL without Seal Wash Outlet valve 1220 1260 Absorber capillary Capillary channel A and B pump head outlet to mixing chamber included Restriction capillary mixing capillary to pressure sensor Capillary SSL 0 17 x 150 mm pressure sensor to damper Mixer Capillary SSL 0 17 x 105 mm connections to solvent mixer Bracket for solvent mixer Purge valve 1260 Capillary ST 0 17 mm x 400 mm S S Capillary ST 0 17 mm x 700 mm S S Waste tube 5 m reorder pack Peristaltic pum
20. 50 This test does not work in emulation mode that is when the G1312B Binary Pump emulates the G1312A Binary Pump In case of an emulated module convert to the original type first see Replacing Module Firmware on page 181 1260 Infinity Binary Pump User Manual 137 138 Test Functions and Calibration Test Principle A solvent can be chosen from available solvent channels and a maximum target pressure can be defined at which the test will be run Typically this is the maximum pressure specified for the pump The test can be run with any solvent compatible to the pump Before the test the pump is flushed with solvent in order to remove air bubbles as air bubbles are compressed during the test and therefore would appear as leaks Using a degasser is highly recommended piston 2 at rear position A pressure piston 1 at rear position piston 2 delivers piston 1 delivers about 100 bar below target pressure gt ie time Initially the pressure is increased to about 100 bar below the target pressure which has been set for the test Then piston 1 is brought to its rear position An increasing flow is delivered by piston 1 In case of a leak the pressure will drop initially as long as the flow rate delivered by the piston is lower than the leak rate As soon as the flow rate of the piston exceeds the leak rate the measured pressure will increase again Therefore the minimum pressure of that
21. 6 of the injection valve and repeat the pressure test Exclude each module one by one to determine which module is leaking If the pump is determined to be the source of the leak run the valve test to identify the defective pump component 1260 Infinity Binary Pump User Manual 129 Test Functions and Calibration Potential Causes of Pressure Test Failure After isolating and fixing the cause of the leak repeat the pressure test to confirm the system is pressure tight Table9 Potential Cause Pump Potential Cause Pump Corrective Action Purge valve open Close the purge valve Loose or leaky fitting Tighten the fitting or exchange the capillary Damaged pump seals or pistons Run the valve test to identify the defective component Loose purge valve Tighten the purge valve nut 14 mm wrench Table 10 Potential Cause Autosampler Potential Cause Autosampler Corrective Action Loose or leaky fitting Tighten or exchange the fitting or capillary Rotor seal injection valve Exchange the rotor seal Damaged metering seal or piston Exchange the metering seal Check the piston for scratches Exchange the piston if required Needle seat Exchange the needle seat Table 11 Potential Cause Column Compartment Potential Cause Column Compartment Corrective Action Loose or leaky fitting Tighten or exchange the fitting or capillary Rotor seal column switching valve Exchange the rotor seal
22. 95 Carbon tetrachloride 110 Chloroform 100 Cyclohexane 118 Ethanol 114 Ethyl acetate 104 Heptane 120 Hexane 150 Isobutanol 100 lsopropanol 100 Methanol 120 1 Propanol 100 Toluene 87 Water 46 1260 Infinity Binary Pump User Manual 89 5 Optimizing Performance How to Optimize the Compressibility Compensation Setting 90 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 92 Status Indicators 94 Power Supply Indicator 94 Module Status Indicator 95 User Interfaces 96 Agilent Lab Advisor Software 97 Overview of the troubleshooting and diagnostic features Apg Agilent Technologies 91 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for
23. Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards 1260 Infinity Binary Pump User Manual 241 14 Appendix Operation 242 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed
24. Handling WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly 60 Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets For d
25. Pump User Manual 175 10 Maintenance Installation of the Solvent Selection Valve Upgrade Kit 1 Remove the front cover sheet by removing both screws Cover sheet Screws 2 Plug in the connector of the solvent selection valve Connector SSV cable Solvent selection valve 3 Install the solvent selection assembly by fixing both indicated screws oy 4 Connect the outlet for solvent A upper row to the inlet valve of the left pump head Connect the outlet for solvent B lower row to the inlet valve of the right pump head Put the solvent bottles into the solvent cabinet Connect the bottle heads of solvents A1 and A2 to the inlets in the upper row see labels on valve assembly Connect the bottle heads of solvents B1 and B2 to the inlets in the lower row see labels on valve assembly 4 A To solvent bottles Inlets Outlets For controlling the solvent selection valve please refer to the online help or user manual of your control software 176 1260 Infinity Binary Pump User Manual Maintenance 10 Exchanging the Solvent Selection Valve If leaking internally crossflow between the ports or if one of the channels is blocked When Tools required p n Description 8710 0899 Screwdriver Pozidriv 1 Parts required p n Description G1381 60000 Preparations Solvent Selection Valve Upgrade Kit Switch off the pump at t
26. Secondary Leak If a leak is found for movement of piston 2 secondary leak the following reasons are possible Probable cause Suggested actions 1 System not flushed properly Flush system for several minutes 2 Degassing efficiency is low Check degasser performance 3 Purge valve not closed or defect Check purge valve 4 Blank nut not installed tightly Tighten or replace blank nut 5 Outlet valve leaking read below Replace outlet valve 6 Leak at piston 2 or seal in chamber 2 Inspect piston replace piston and or seal Primary Leak If a leak is found for movement of piston 1 primary leak any leak described for piston movement 2 will cause a failure for piston 1 as well as the liquid can move through the outlet valve to chamber 2 Such cases need to be identified as described before Additionally following causes are possible Probable cause Suggested actions 1 Leak at piston 1 or seal in chamber 1 Inspect piston replace piston and or seal 2 Leak at inlet valve Replace inlet valve or inlet valve cartridge AIV only Internal Outlet Valve Leak A leak of the outlet valve will be identified separately internal outlet valve leak by calculating the difference between leak rate 1 and leak rate 2 If the second leak rate is higher than the first one this is due to a flow back through the outlet valve Probable cause Suggested actions 1 Leak at outlet valve Replace the part which has failed and re run the test 1260 Infinity B
27. Turn pump default unchecked If this option is checked the pump will turn off before air is aspirated However off the residual solvent volume has been calculated for 1 L solvent bottles and may be too small for large bottles or other vessels 68 1260 Infinity Binary Pump User Manual Solvent Information Using the Pump 4 Observe the following recommendations on the use of solvents e Follow recommendations for avoiding the growth of algae see Algae Growth in HPLC Systems on page 74 e Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters e Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Materials in Flow Path Following materials are used in the flow path of this module Table 7 Materials in flow path Part Materials SSV PEEK FFKM Active inlet valve SST sapphire ruby ceramic PTFE Outlet valve SST gold ruby ZrO5 based ceramic tantalum Adapter SST gold Pump head body SST Pistons Sapphire Piston seals wash seals Pressure sensor Purge valve Damping unit Capillaries fittings Tubings PTFE SST reversed phase or UHMW PE SST normal phase SST SST gold PTFE ceramic SST gold SST PTFE 1260 Infinity Binary Pump User Manual
28. WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product g Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1260 Infinity Binary Pump User Manual 243 14 Appendix Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren
29. be reached the error message is generated Probable cause 1 Disconnected or defective cable 2 Blocked active inlet valve 3 Mechanical blockage of the module 4 Defective pump drive assembly 5 Defective main board 118 Suggested actions Please contact your Agilent service representative Exchange the active inlet valve Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly Please contact your Agilent service representative Please contact your Agilent service representative 1260 Infinity Binary Pump User Manual Error Information 7 Pump Head Missing Error ID 2202 2212 Pump Head Missing Pump channel A B Pump Head Missing Pump channel B The pump head end stop in the pump was not found When the pump restarts the metering drive moves forward to the mechanical end stop Normally the end stop is reached within 20 s indicated by an increase in motor current If the end point is not found within 20 s the error message is generated Probable cause Suggested actions 1 Pump head not installed correctly screws Install the pump head correctly Ensure nothing not secured or pump head not seated e g capillary is trapped between the pump correctly head and body 2 Broken piston Exchange the piston 1260 Infinity Binary Pump User Manual 119 7 Error Information Index Limit Error ID 2203 2213 Index Limit Pump chan
30. com 1260 Infinity Binary Pump User Manual 247 Index Index 8 bit configuration switch without On Board LAN 228 A absorber capillary 14 active inlet valve 194 active seal wash 10 81 adapter 52 Agilent Lab Advisor software 97 Agilent Lab Advisor 97 128 Agilent oninternet 247 AIV fuse 113 algae growth 76 algae 61 alternative seal material 82 ambient non operating temperature 25 ambient operating temperature 25 analog signal output 27 analog signal 225 analog cable 204 apg remote 226 assembly bottle head 197 AUTO mode 17 B ball screw drive 13 battery safety information 244 BCD board 248 external contacts 232 BCD board 180 cable 209 bench space 24 blockage 116 board HP JetDirect card 234 boards LAN card 234 bottle head assembly 197 197 buffer application 61 buffer solutions 10 C cable analog 204 BCD 209 CAN 211 external contact 212 interface 47 LAN 211 remote 206 RS 232 213 cables analog 202 BCD 202 CAN 203 external contact 203 LAN 203 overview 202 remote 202 RS 232 203 CAN cable 211 capillary absorber 14 changing solvents 57 cleaning 151 Communication settings RS 232C 229 compensation sensor open 106 compensation sensor short 107 compensation compressibility 26 composition accuracy 27 composition precision 27 composition range 27 composition ripple 133 compressibility calibration 133 compressibility compensation 26 87 condensation 24 configur
31. drawn volume into the system For pump specifications see Performance Specifications on page 26 1260 Infinity Binary Pump User Manual CAUTION Introduction to the Binary Pump 1 What is Pump Elasticity Compensation The flow path of the pump consists of pump chambers sapphire pistons polymer seals stainless steel tubing of different dimension pressure sensor and so forth All of these parts deform when pressurized The sum of this deformation is called pump elasticity Let us look at a practical example Piston 1 draws solvent at ambient pressure The movement direction is reversed and the piston 1 now compresses the solvent until the operating pressure of the HPLC system is reached The outlet valve opens and solvent is pumped by piston 1 into pump chamber 2 Due to two factors the solvent volume that is delivered into the system at high pressure is smaller than it is supposed to be 1 The solvent is compressible 2 The pump has a certain elasticity which causes its internal volume to increase with pressure In order to compensate for these two influences their contributions must be known An elasticity calibration allows separating pump properties from solvent properties and therefore allows transferring solvent properties which have been obtained from one pump to another pump with different elasticity Elasticity calibration is done with a solvent which properties compressibility thermal expansion are well k
32. is off the module is turned off 7 Purge the pump see Initial Priming on page 54 The pump is shipped with default configuration settings To change these settings refer to Setting the 8 bit Configuration Switch without On board LAN on page 228 1260 Infinity Binary Pump User Manual 47 3 Installing the Pump Flow Connections with Solvent Selection Valve Parts required p n Description 1 Other modules 1 G1311 68755 Accessory Kit 2 wrenches 1 4 5 16 inch for capillary connections Preparations Pump is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can carry health risks gt Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Remove the front cover by pressing the snap fasteners on both sides Figure 16 Removing the Front Cover 48 1260 Infinity Binary Pump User Manual Installing the Pump 3 2 If available place the online degasser on top of the pump Place the solvent cabinet on top of the module 4 Set the four bottles into the solvent cabinet and screw a bottle head assembly onto each bottle 5 Connect the solvent tubes from the bottle head assemblies to the inlet connectors Al
33. m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m 1260 Infinity Binary Pump User Manual 203 12 Identifying Cables Analog cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog an Center Center Analog 204 1260 Infinity Binary Pump User Manual Agilent Module to General Purpose Identifying Cables 12 p n 01046 60105 Pin Agilent Signal Name module Not connected Black Analog Red Analog S e 1260 Infinity Binary Pump User Manual 205 12 Identifying Cables Remote Cables 206 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators
34. of the binary pump A blocked filter therefore does not necessarily affect the high pressure readings of the pump The pressure readings cannot be used to check whether the filters are blocked or not If the solvent cabinet is placed on top of the binary pump the filter condition can be checked in the following way Remove the solvent inlet tube from the inlet port of the solvent selection valve or the adapter at the active inlet valve If the filter is in good condition the solvent will freely drip out of the solvent tube due to hydrostatic pressure If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can carry health risks gt Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Cleaning the Solvent Filters Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 85 for one hour e Thoroughly flush the filter with HPLC grade water remove all nitric acid some capillary columns can be damaged by nitric acid e Replace the filter Never use the system without solvent filter installed
35. phase solvents 82 61 153 purge valve radio interference 245 reassembling the pump head 167 recommended pH range 26 remote cable 206 remove damper and mixer 84 removing the pump head 157 removing pump head assembly 153 repair procedures 153 repairs definition of 148 introduction 148 replacing firmware 181 181 warnings and cautions 148 results pressure test ripple composition 133 pressure 133 RS 232C cable 213 communication settings 229 132 129 1260 Infinity Binary Pump User Manual S safety class 241 safety information lithium batteries 244 safety general information 241 standards 25 symbols 240 sapphire piston 13 seal wash wash solvent 81 when touse 81 seal wear counter 162 seals alternative material 82 normal phase 82 servo restart failed 118 setable flow range 26 shutdown 103 sieve 153 173 simple repair procedures 153 site requirements 21 power cords 23 snap fastener 48 51 solubility gas 61 solvent cabinet 49 61 solvent calibration 133 solvent compressibility calibration 93 134 solvent filters checking 77 cleaning 77 prevent blocking 76 solvent inlet filter 61 solvent selection valve 10 48 153 177 solvent zero counter 109 solvents change 57 sound emission 246 special interfaces 227 special settings boot resident 231 forced cold start 231 specification physical 25 specifications analog signal output 27 communications 27 control and data evaluation 27 p
36. probable causes and suggested actions how to recover from error conditions 1260 Infinity Binary Pump User Manual 3 In This Guide 8 Test Functions and Calibration This chapter explains all test functions that are available for the binary pump 9 Diagnostic Signals This chapter explains all diagnostic signals of the binary pump 10 Maintenance This chapter describes the maintenance of the module 11 Parts and Materials for Maintenance This chapter lists all parts and tools that are required for maintenance 12 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules 13 Hardware Information This chapter provides detailed technical information about your binary pump 14 Appendix This appendix provides general safety and environmental information 1260 Infinity Binary Pump User Manual Contents Contents 1 Introduction to the Binary Pump 9 Instrument and Operation 10 Overview of the Hydraulic Path 13 Leak and Waste Handling 18 2 Site Requirements and Specifications 21 Site Requirements 22 Physical Specifications 25 Performance Specifications 26 3 Installing the Pump 29 Unpacking the Binary Pump 30 Optimizing the Stack Configuration 33 Installation Information on Leak and Waste Handling 41 Installing the Binary Pump 45 Flow Connections with Solvent Selection Valve 48 Flow Connections without Solvent Selection Valve 51 Priming the
37. rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable 1260 Infinity Binary Pump User Manual Hardware Information 13 The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table 15 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 a 2 RX TX 3 PAN 3 1X DTR 4 ay 4 DTR GND 5 X 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 A 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 37 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board 1260 Infinity Binary Pump User Manual 225 13 Hardware Information APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is
38. recompress the solvent to operating pressure During this time frame no solvent would be delivered into the system and as a result a high pressure fluctuation known as pressure ripple would be observed 1260 Infinity Binary Pump User Manual Introduction to the Binary Pump 1 When both solvent compressibility at the current operating pressure and pump elasticity are known the pump can automatically correct for the missing volume by drawing the appropriate larger solvent volume at ambient pressure and speed up the piston during the recompression phase in the first pump chamber As a result the pump delivers the accurate volume with any calibrated solvent at any pressure at a greatly reduced pressure ripple For applications that require lowest transition volume of the pump damper and mixer can be bypassed For compatibility with older methods from G1312A Binary Pumps the previous one point compressibility compensation is available too However since the compressibility is a non linear function one single compressibility value per solvent will only give good results at one particular pressure How Does Variable Stroke Volume Work The smaller the solvent volume in the pump chamber is the faster it can be recompressed to operating pressure The binary pump allows to manually or automatically adjust the pump stroke volume of the first piston in the range of 20 100 uL Due to the compression of the solvent volume in the first pump c
39. the pump outlet capillary pump to injection device to the outlet of the purge valve 1260 Infinity Binary Pump User Manual Installing the Pump 3 Flow Connections without Solvent Selection Valve 8 Purge your system before first use see Initial Priming on page 54 Bottle head assembly Solvent cabinet Degasser Pressure sensor Static mixer Purge valve Pump head B Pump head A Adapter Active inlet valve B Active inlet valve A Waste tubing Waste outlet Outlet capillary to autosampler Figure 19 Flow Connection of Binary Pump without Solvent Selection Valve 1260 Infinity Binary Pump User Manual 53 3 Installing the Pump Priming the System Initial Priming When Parts required Preparations Before a degasser or solvent tubing can be used it is necessary to prime the system lsopropanol is recommended as priming solvent due to its miscibility with nearly all HPLC solvents and its excellent wetting properties Description 1 lsopropanol Connect all modules hydraulically as described in the respective module manuals Fill each solvent bottle with 100 mL isopropanol Switch the system on When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can carry health risks gt Observe appropriate safety procedures for example wear goggles safety gloves and protect
40. troubleshooting and operational verification after exchanging internal components see Tests and Calibrations Pressure Test The Pressure Test is a quick test designed to determine the pressure tightness of the system i e the high pressure flow path between pump and column After exchanging flow path components e g pump seals or injection seal use this test to verify the system is pressure tight see System Pressure Test on page 126 92 1260 Infinity Binary Pump User Manual Troubleshooting and Diagnostics 6 Solvent Compressibility Calibration Solvent compressibility is a function of solvent type and pressure In order to optimize flow accuracy and pressure ripple the compressibility of the solvent must be considered The binary pump firmware contains compressibility parameters for most commonly used solvents A compressibility calibration function is available to generate compressibility data for unlisted solvents see Binary Pump Solvent Compressibility Calibration on page 133 The compressibility data are stored in an XML file and can be transferred to other G1312B pumps Pump Elasticity Calibration Various parts in the flow path of the binary pump have a certain elasticity which needs to be compensated to obtain the lowest pressure flow and composition ripple possible This is done by running an elasticity calibration after maintenance and major repairs For details see Pump Elasticity Calibration o
41. turns the function off The volume one pump piston delivers per stroke In general a smaller stroke volume results in lower pump ripple The Auto setting adjusts the strokes dynamically to the lowest possible value The strokes can be set individually for pump heads A and B For best performance check option Use enhanced compressibility calibration With this option the pump will use solvent data libraries provided by Agilent or data generated by using solvent compressibility calibrations see Running the Solvent Compressibility Calibration on page 134 For details on solvent compressibility see Binary Pump Solvent Compressibility Calibration on page 133 For backward compatibility to 400 bar pumps the solvent compressibility can still be set manually for each channel when the box is unticked 66 1260 Infinity Binary Pump User Manual Using the Pump 4 Data Curves The binary pump provides the possibility to store the following operational data in the data file of the Agilent data system e Solvent percentage for each channel pump flow pressure The pressure data curve is generated from the pressure sensor readings while A B and flow are calculated from the method settings of the pump For details please refer to the online help or manual of your instrument control software Bottle Filling CAUTION The pump offers a powerful feature to monitor the liquid level in the solvent bottles With tota
42. 1260 Infinity Binary Pump User Manual 19 1 20 Introduction to the Binary Pump Leak and Waste Handling 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual e00 ee 2 r Site Requirements and Specifications Site Requirements 22 Physical Specifications 25 Performance Specifications 26 This chapter provides information about site requirements and specifications for the binary pump ott Agilent Technologies 21 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 1 on page 25 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover
43. 260 Infinity Binary Pump User Manual Error Information 7 Initialization Failed Error ID 2207 2217 Initialization Failed Pump channel A B Initialization Failed Pump channel B The module failed to initialize successfully within the maximum time window A maximum time is assigned for the complete pump initialization cycle If the time is exceeded before initialization is complete the error message is generated Probable cause Suggested actions 1 Blocked active inlet valve Exchange the active inlet valve 2 Defective pump drive assembly Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 1260 Infinity Binary Pump User Manual 123 7 Error Information Module Error Messages 124 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual e Test Functions and Calibration e System Pressure Test 126 Positioning the Blank Nut 127 Running the System Pressure Test 128 Evaluating the Results 129 Valve Test 131 Running the Valve Test 132 Evaluating the Results 132 Binary Pump Solvent Compressibility Calibration 133 Running the Solvent Compressibility Calibration 134 Pump Elasticity Calibration 135 Running the Pump Elasticity Calibration 136 Leak Rate Test 137 Running the Test 139 Evaluating the Results 140 Potential Causes of Leak Rate Test Failure 141 This chapter explains all test functions that are a
44. 69 4 70 Using the Pump Material Information Materials in the flow path are carefully selected based on Agilent s experiences in developing highest quality instruments for HPLC analysis over several decades These materials exhibit excellent robustness under typical HPLC conditions For any special conditions please consult the material information section or contact Agilent Disclaimer Subsequent data were collected from external resources and are meant as a reference Agilent cannot guarantee the correctness and completeness of such information Data is based on compatibility libraries which are not specific for estimating the long term life time under specific but highly variable conditions of UHPLC systems solvents solvent mixtures and samples Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Apart from pure chemical corrosion other effects like electro corrosion electrostatic charging especially for non conductive organic solvents swelling of polymer parts etc need to be considered Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually accelerates at higher temperatures If in doubt please consult technical literature on chemical compatibility of materials PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance m
45. Binary Pump User Manual Hardware Information 13 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems The interface board slot is used for external contacts and BCD bottle number output or LAN connections e The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch e The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1260 Infinity Binary Pump User Manual 219 13 Hardware Information Electrical Connections Rear view of the m
46. D VVV is the revision number for example 102 is revision 1 02 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Main FW update Resident System Main System Resident FW Update Figure 35 Firmware Update Mechanism 1260 Infinity Binary Pump User Manual 217 13 Hardware Information 218 Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 1260 Infinity
47. D ESD can damage electronic boards and components gt In order to prevent damage always use an ESD protection when handling electronic boards and components 1 Disconnect cables from the interface board connectors 2 Loosen the screws Slide out the interface board from the module Se y amp __ SA Figure 24 Exchanging the Interface Board 3 Install the new interface board Secure the screws 4 Reconnect the cables to the board connector 180 1260 Infinity Binary Pump User Manual Maintenance Replacing Module Firmware When Tools required OR OR Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool 10 To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Upda
48. Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1260 Infinity Binary Pump User Manual Appendix 14 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment
49. Limit Exceeded Error ID 2517 Temperature Limit Exceeded 0 Pump channel A Temperature Limit Exceeded 1 Pump channel B The temperature of one of the motor drive circuits is too high The processor continually monitors the temperature of the drive circuits on the main board If excessive current is being drawn for long periods the temperature of the circuits increases If the temperature exceeds the upper limit the error message is generated Probable cause Suggested actions 1 High friction partial mechanical blockage Remove the pump head assembly Ensure there in the pump drive assembly is no mechanical blockage of the pump head assembly or pump drive assembly 2 Partial blockage of the flowpath in front of Ensure the outlet valve is not blocked the damper 3 Defective pump drive assembly Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative 1260 Infinity Binary Pump User Manual 115 7 Error Information Motor Drive Power Error ID 2041 2042 Motor Drive Power Pump channel A B Motor Drive Power Pump channel B The current drawn by the pump motor exceeded the maximum limit Blockages in the flow path are usually detected by the pressure sensor in the damper which result in the pump switching off when the upper pressure limit is exceeded If a blockage occurs before the damper the pressure increase cannot be detected by the pressure sens
50. Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 1260 Infinity Binary Pump User Manual 207 12 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL 7 White 1 Digital ground a Ol ou Brown 2 Prepare run Low 00 pes DE KEY Gray 3 Start Low oo o0 S iE Blue 4 Shut down Low og Pink 5 Not oa connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 208 1260 Infinity Binary Pump User Manual BCD Cables Identifying Cables 12 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 pea Violet 2 BCD 7 80 AA Blue 3 BCD 6 40 a ee Yellow 4 BCD 4 10 Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 1260 Infinity Binary Pump User Manual 209 12 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit
51. Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available A and the user should not touch it when heated up WARNING ahaa alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 240 1260 Infinity Binary Pump User Manual Appendix 14 General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING
52. System 54 4 Usingthe Pump 59 Leak and Waste Handling 60 Hints for Successful Use of the Binary Pump 61 Setting up the Pump with the G4208A Instant Pilot 62 Setting up the Agilent 1260 Infinity Binary Pump G1312B with the Instrument Control Interface 63 Solvent Information 69 Algae Growth in HPLC Systems 74 Prevent Blocking of Solvent Filters 76 1260 Infinity Binary Pump User Manual Contents Optimizing Performance 79 When to Use a Vacuum Degasser 80 When to Use the Active Seal Wash Option 81 When to Use Alternative Seals 82 When to Use the Low Volume Mixer 83 When to Remove Damper and Mixer 84 How to Optimize the Compressibility Compensation Setting 87 Troubleshooting and Diagnostics 91 Overview of the Module s Indicators and Test Functions 92 Status Indicators 94 User Interfaces 96 Agilent Lab Advisor Software 97 Error Information 99 What Are Error Messages 101 General Error Messages 102 Module Error Messages 109 Test Functions and Calibration 125 System Pressure Test 126 Valve Test 131 Binary Pump Solvent Compressibility Calibration 133 Pump Elasticity Calibration 135 Leak Rate Test 137 Diagnostic Signals 143 Analog Pressure Output 144 Diagnostic Signals in Chromatographic Data Systems 145 1260 Infinity Binary Pump User Manual Contents 10 Maintenance 147 Introduction to Maintenance and Repair 148 Warnings and Cautions 149 Cleaning the Module 151 Overview of Maintenance and S
53. a ZDV fitting Prepare bottles with appropriate intermediate solvents see Table 3 on page 58 1 If the channel is not filled with buffer proceed to step 4 Place the solvent intake filter into a bottle of water Flush the channel at a flow rate suitable for the installed tubing typically 3 5 mL min for 10 min 4 Modify the flow path of your system as required for your application For delay volume optimization see the Binary LC System User Guide Buffer salt of aqueous buffers may precipitate in residual isopropanol Capillaries and filter may be clogged by precipitating salt gt Flush solvent lines containing high concentration of salts first with water before introducing organic solvent gt Do not perform steps 5 to 7 for channels running with aqueous buffer as solvent 5 Replace the solvent bottle by a bottle of isopropanol 6 Flush the channel at a flow rate suitable for the installed tubing typically 3 5 mL min for 5 min 7 Swap the bottle of isopropanol with a bottle of solvent for your application 8 Repeat steps 1 to 7 for the other channel s of the pump 9 Install the desired column set the required composition and flow rate for your application and equilibrate the system for approx 10 minutes prior to starting a run 1260 Infinity Binary Pump User Manual 57 3 58 Installing the Pump Table 3 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an inst
54. age 177 Yearly or if the frit shows indication of contamination or blockage If internally leaking During yearly maintenance Yearly or if pump performance indicates seal wear Yearly or if pump performance indicates seal wear If leaking externally If solenoid is defective If internally leaking If internally leaking If solenoid is defective A pressure drop of gt 10 bar in low delay volume configuration and gt 20 bar in standard configuration across the frit 5 mL min H 20 with purge valve open indicates blockage Solvent dripping out of waste outlet when valve is closed Necessary to get access to pump seals and pistons Leaks at lower pump head side unstable retention times pressure ripple unstable run Valve Test for verification Seal life time shorter than normally expected check pistons while changing the seals Only necessary when Seal Wash Option is installed Leaks at lower pump head side loss of wash solvent Error messages Inlet Valve Fuse or Inlet Valve Missing Pressure ripple unstable run Valve Test for verification Cross port flow Error message Valve Failed 1260 Infinity Binary Pump User Manual 153 10 Maintenance Exchanging the Purge Valve Frit or the Purge Valve When Tools required OR Parts required Preparations 154 Frit when piston seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar in low dela
55. agnostic Signals Diagnostic Signals in Chromatographic Data Systems 146 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual 10 Maintenance Introduction to Maintenance and Repair 148 Warnings and Cautions 149 Cleaning the Module 151 Overview of Maintenance and Simple Repairs 152 Maintenance Procedures 153 Exchanging the Purge Valve Frit or the Purge Valve 154 Removing the Pump Head Assembly 157 Maintenance of a Pump Head without Seal Wash 159 Maintenance of a Pump Head with Seal Wash 163 Reinstalling the Pump Head Assembly 167 Seal Wear in Procedure 169 Exchanging the Active Inlet Valve AIV or its Cartridge 170 Exchanging the Outlet Valve 173 Installation of the Solvent Selection Valve Upgrade Kit 175 Exchanging the Solvent Selection Valve 177 Exchanging the Optional Interface Board 180 Replacing Module Firmware 181 This chapter describes the maintenance of the module ate Agilent Technologies 147 10 Maintenance Introduction to Maintenance and Repair The pump is designed for easy repair The most frequent repairs such as piston seal replacement and purge valve frit exchange can be done from the front side without removing the pump from the system stack These repairs are described in Overview of Maintenance and Simple Repairs on page 152 148 1260 Infinity Binary Pump User Manual Maintenance 10 Warnings and Cautions WARNING Toxic flammable and hazardous solvents samp
56. aintenance Removing the Pump Head Assembly 3 Using a 4 mm hexagonal key stepwise loosen and remove the two pump head screws Pump head screws 158 1260 Infinity Binary Pump User Manual Maintenance 10 Maintenance of a Pump Head without Seal Wash When In case of maintenance or pump head internal leaks Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2411 Hex key 3 mm12 cm long 8710 2392 Hex key 4 mm15 cm long T handle 01018 23702 Insert tool Parts required p n Description 1 5063 6589 Piston seal PTFE carbon filled black pack of 2 default OR 1 0905 1420 PE seals pack of 2 1 5063 6586 Sapphire piston Preparations Switch off the pump at the main power switch Remove the front cover to have access to the pump heads e Removing the Pump Head Assembly on page 157 Both pump head assemblies use the same internal components In addition pump head A is fitted with the purge valve The following procedure describes the removal and disassembly of pump head A left For pump head B right proceed in the same way and skip steps that deal with the purge valve 1260 Infinity Binary Pump User Manual 159 10 Maintenance Maintenance of a Pump Head without Seal Wash 1 Place the pump head on a flat surface Loosen the lock screw two turns and while holding the lower half of the assembly piston housing carefully lift up the pump housing and remove it from the piston housing Lock screw
57. all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system 1260 Infinity Binary Pump User Manual Error Information 7 Leak Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Loose or leaking purge valve active inlet Ensure pump components are seated correctly valve or outlet valve If there are still signs of a leak exchange the appropriate seal purge valve active inlet valve outlet valve 4 Defective pump seals Exchange the pump seals Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limi
58. allation lsopropanol Best solvent to flush air out of the system When switching between lsopropanol Miscible with almost all reverse phase and normal solvents phase both times After an installation To clean the system when using buffers After changing aqueous solvents After the installation of normal phase seals PE seals pack of 2 0905 1420 Ethanol or methanol HPLC grade water HPLC grade water Hexane 5 isopropanol Alternative to isopropanol second choice if no isopropanol is available Best solvent to re dissolve buffer crystals Best solvent to re dissolve buffer crystals Good wetting properties 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual 4 Using the Pump Leak and Waste Handling 60 Hints for Successful Use of the Binary Pump 61 Setting up the Pump with the G4208A Instant Pilot 62 Setting up the Agilent 1260 Infinity Binary Pump G1312B with the Instrument Control Interface 63 Overview 63 Setup of Basic Pump Parameters 64 Pump Control 65 Auxiliary Pump Parameters 66 DataCurves 67 Bottle Filling 67 Solvent Information 69 Algae Growth in HPLC Systems 74 How to Prevent and or Reduce the Algae Problem 75 Prevent Blocking of Solvent Filters 76 Checking the Solvent Filters 77 Cleaning the Solvent Filters 77 This chapter explains the operational parameters of the binary pump Apg Agilent Technologies 59 4 Using the Pump Leak and Waste
59. ant Pilot G4208A Agilent Lab Advisor Pressure Test Yes Yes Valve Test No Yes Solvent compressibility No Yes calibration Pump elasticity calibration No Yes 1260 Infinity Binary Pump User Manual Troubleshooting and Diagnostics 6 Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files The Instrument Utilities is a basic version of the Lab Advisor with limited functionality required for installation use and maintenance No advanced repair troubleshooting and monitoring fun
60. ap including the gold seal NOTE Before reinstallation always check the gold seal in the seal cap A deformed seal cap should be exchanged 6 Insert the purge valve into the purge valve holder and orient the waste outlet nozzle downward 7 Tighten the purge valve and reconnect outlet capillary and waste tubing 156 1260 Infinity Binary Pump User Manual Maintenance 10 Removing the Pump Head Assembly When Exchanging pump seals Exchanging pistons Exchanging seals of the seal wash option Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2411 Hex key 3 mm12 cm long 8710 2392 Hex key 4 mm15 cm long T handle 5023 0240 Hex driver 14 slitted Preparations Switch off the pump at the main power switch CAUTION Damage of the pump drive Starting the pump when the pump head is removed may damage the pump drive gt Never start the pump when the pump head is removed Both pump head assemblies use the same internal components In addition pump head A is fitted with the purge valve The following procedure describes the removal and disassembly of pump head A left For pump head B right proceed in the same way and skip steps that deal with the purge valve 1 Remove the front cover 2 Disconnect the capillaries at the back of the purge valve holder the pump head adapter and the tube at the active inlet valve Beware of leaking solvents 1260 Infinity Binary Pump User Manual 157 10 M
61. ase contact your Agilent service representative 4 Improperly positioned cables or wires Please contact your Agilent service obstructing fan blades representative 1260 Infinity Binary Pump User Manual 107 7 Error Information Open Cover Error ID 0205 The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during Please contact your Agilent service operation representative 2 Foam not activating the sensor Please contact your Agilent service representative 3 Defective sensor or main board Please contact your Agilent service representative 4 Rear of the module is exposed to strong Ensure that the rear of module is not directly direct sunlight exposed to strong sunlight 108 1260 Infinity Binary Pump User Manual Module Error Messages Solvent Zero Counter Error ID 2055 The error message is triggered if the falls below the set limit Probable cause 1 Volume in bottle below specified volume 2 Incorrect setting Pressure Above Upper Limit Error ID 2014 2500 Error Information 7 remaining volume in a solvent bottle Suggested actions Refill bottles and reset solvent counters Make sure the set solvent volume matches the actual bottle filling and set the shutoff limit to a reasonable value e g 100 mL for 1 L bottl
62. assembly The outlet of the purge valve assembly is then connected to the attached chromatographic system When turned on the pump runs through an initialization procedure to determine the upper dead center of the first piston of both pump channels The first piston moves slowly upwards to the mechanical stop of the pump head and from there it moves back a predetermined path length The controller stores this piston position in memory After this initialization the pump starts operation with the set parameters for the two pump channels The active inlet valve is opened and the down moving piston draws solvent into the first pump head At the same time the second piston is moving upwards delivering into the system After a controller defined stroke length depending on the flow rate the drive motors are stopped and the active inlet valve is closed The motor direction is reversed and moves the first piston up until it reaches the stored upper limit and at the same time moving the second piston downwards Then the sequence starts again moving the pistons up and down between the two limits During the delivery stroke of the first piston the solvent in the pump head is pressed through the outlet valve into the second pumping unit The second piston draws in half of the volume displaced by the first piston and the remaining half volume is directly delivered into the system During the drawing stroke of the first piston the second piston delivers the
63. ation low flow rates 61 connections flow 51 48 control and data evaluation 27 D damaged parts 31 damper when to remove 84 data evaluation and control 27 defect on arrival 30 delay volume 26 84 delivery checklist 31 design 11 diagnostic signals 93 dimensions 25 dual piston in series design 11 1260 Infinity Binary Pump User Manual E electrical connections descriptions of 219 electronic fuse of SSV open 113 electronic waste 243 electrostatic discharge ESD 180 EMF early maintenance feedback 237 encoder missing 117 error messages AlV fuse 113 compensation sensor open 106 compensation sensor short 107 electronic fuse of SSV open 113 encoder missing 117 fan failed 107 ignition without cover 108 108 index adjustment 121 index limit 120 index missing 122 initialization failed 123 inlet valve missing 117 eak sensor open 105 eak sensor short 106 eak 105 ost CAN partner 104 missing pressure reading 112 motor drive power 116 overpressure 128 134 132 136 pressure above upper limit 109 pressure below lower limit 110 pressure signal missing 110 pump head missing 119 remote timeout 104 servo restart failed 118 shutdown 103 stroke length 122 temperature limit exceeded 115 temperature out of range 114 timeout 102 valve failed 111 wrong pump configuration 112 error solvent zero counter 109 exchanging inlet valve 153 interface board 180 outlet valve sieve 153 173 outlet valve 153 173 pistons 153 153 pum
64. bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia Zirconium Oxide ZrO Zirconium Oxide is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fluorinated polymers PTFE PFA FEP FFKM Fluorinated polymers like PTFE polytetrafluorethylene PFA perfluoroalkoxy and FEP fluorinated ethylene propylene are inert to almost all common acids bases and solvents FFKM is perfluorinated rubber which is also resistant to most chemicals As an elastomer it may swell in some organic solvents like halogenated hydrocarbons TFE PDD copolymer tubings which are used in all Agilent degassers except G1322A are not compatible with fluorinated solvents like Freon Fluorinert or Vertrel They have limited life time in the presence of Hexafluoroisopropanol HFIP To ensure the longest possible life with HFIP it is best to dedicate a particular chamber to this solvent not to switch solvents and not to let dry out the chamber For optimizing the life of the pressure sensor do not leave HFIP in the chamber when the unit is off Sapphire Ruby and Al 03 based ceramics Sapphire ruby and ceramics based on aluminum oxide AlyOg are inert to almost all comm
65. contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused Board identification 12 Processor BCD BCD interface register connector Line driver RFI filter 250mA External 4x External contacts lt contact connector DRF filter 232 1260 Infinity Binary Pump User Manual Hardware Information 13 There are general purpose cables available to connect the BCD output see BCD Cables on page 209 and the external outputs see External Contact Cables on page 212 to external devices Table 24 Detailed connector layout 1200 Pin Signal name BCD digit 1 BCD 5 20 2 BCD 7 80 3 BCD 6 40 4 BCD 4 10 5 BCD 0 1 6 BCD 3 8 7 BCD 2 4 8 BCD 1 2 9 Digital ground 10 BCD 11 800 11 BCD 10 400 12 BCD 9 200 13 BCD 8 100 15 5V Low 1260 Infinity Binary Pump User Manual 233 13 Hardware Information LAN Communication Interface Board The Agilent modules have one optional board slot that allows to add an interface board to the modules Some modules do not have this interface slot Refer to Interfaces on page 221 for details p n Description G1369B or Interface board LAN G1369 60002 OR G1369C or Interface board LAN G1369 60012 One board is required per Agilent 1260 Infinity instrument It is recommended to add the LAN board to the detector with highest data rate For the configuration of the G1369 LAN Communication Interface card refer to its docu
66. ctionality is included 1260 Infinity Binary Pump User Manual 97 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 98 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual 7 Error Information What Are Error Messages 101 General Error Messages 102 Timeout 102 Shutdown 103 Remote Timeout 104 Lost CAN Partner 104 Leak 105 Leak Sensor Open 105 Leak Sensor Short 106 Compensation Sensor Open 106 Compensation Sensor Short 107 Fan Failed 107 OpenCover 108 Module Error Messages 109 Solvent Zero Counter 109 Pressure Above Upper Limit 109 Pressure Below Lower Limit 110 Pressure Signal Missing 110 Valve Failed 111 Missing Pressure Reading 112 Wrong Pump Configuration 112 Electronic Fuse of SSV Open 113 AIV Fuse 113 Temperature Out of Range 114 Temperature Limit Exceeded 115 Motor Drive Power 116 Encoder Missing 117 Inlet Valve Missing 117 Re Agilent Technologies 99 7 100 Error Information Servo Restart Failed 118 Pump Head Missing 119 120 Index Adjustment 121 122 122 Initialization Failed 123 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 1260 Infinity Binary Pump User Manual Error Information 7 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which re
67. d Preparations CAUTION Running the Solvent Compressibility Calibration from the Agilent Lab Advisor If a solvent is not available in the list of pre calibrated solvents the solvent compressibility calibration allows to generate appropriate compressibility data p n Description 8710 0510 Wrench 1 4 5 16 inch open p n Description G1312 67500 Calibration capillary assembly Place a bottles with solvent to be calibrated in channel A resp A1 if a solvent selection valve is installed Avoid inaccurate pump elasticity calibration This would lead into invalid and not portable solvent compressibility data gt Make sure to perform accurate pump elasticity calibration 134 Make absolutely sure that the pump is very thoroughly flushed with the solvent to be calibrated before starting the procedure Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the calibration to fail 1 Select the solvent from the test selection menu 2 Start the test and follow the instructions Make sure to release the pressure by opening the purge valve when the test has completed Otherwise the pump may generate an overpressure error 1260 Infinity Binary Pump User Manual Test Functions and Calibration 8 Pump Elasticity Calibration CAUTION Description The flow path components of the binary pump have an inherent and pressure dependent elasticity which differs from pump to pump When t
68. d to keep the pressure constant is directly translated into a leak rate 1260 Infinity Binary Pump User Manual Test Functions and Calibration 8 Positioning the Blank Nut To test the complete system s pressure tightness the blank nut should be positioned at the column compartment outlet or the outlet of the last module before the detector If a specific component is suspected of causing a system leak place the blank nut immediately before the suspected component and then run the System Pressure Test again If the test passes the defective component is located after the blank nut Confirm the diagnosis by placing the blank nut immediately after the suspected component The diagnosis is confirmed if the test fails 1260 Infinity Binary Pump User Manual 127 8 Test Functions and Calibration Running the System Pressure Test Running the test from the Agilent Lab Advisor When The test should be used when problems with small leaks are suspected or after maintenance of flow path components e g pump seals injection seal to prove pressure tightness up to 600 bar Tools required p n Description 8710 0510 Wrench 1 4 5 16 inch open Blank nut 1 16 inch Preparations Place two bottles of HPLC grade water in channels A and B A1 and B1 if the pump is equipped with a solvent selection valve Make absolutely sure that all parts of the flow path that are part of the test are very thoroughly flushed with water before starting
69. dicates a not ready condition The module is in a not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e a red LED may indicate a problem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold start see Special Settings on page 231 Then try a firmware update see Replacing Module Firmware on page 181 If this does not help a main board replacement is required 1260 Infinity Binary Pump User Manual 95 96 6 Troubleshooting and Diagnostics User Interfaces Depending on the user interface the available tests vary Some descriptions are only available in the Service Manual Test Inst
70. e a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to cou
71. e about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 1260 Infinity Binary Pump User Manual 45 3 Installing the Pump Installing the Binary Pump 1 Place the module on the bench in a horizontal position 2 Ensure the power switch on the front of the pump is OFF switch stands out Status Indicator 1 s gto Weeinalegtes CD eter E Q Power switch Serial number Figure 14 Front of Binary Pump 3 Connect the power cable to the power connector at the rear of the module 46 1260 Infinity Binary Pump User Manual Installing the Pump 3 Installing the Binary Pump 4 Connect the required interface cables to the rear of the module Configuration switch Slot for interface board Analog output CAN Bus Product We GNC TH Sent Me DESmut 10 Power plug Figure 15 Rear of the Binary Pump 5 Connect the capillary solvent tubes and waste tubings see Flow Connections with Solvent Selection Valve on page 48 or Flow Connections without Solvent Selection Valve on page 51 6 Press the power switch to turn on the module NOTE The power switch stays pressed in and a green indicator lamp in the power switch is on when the module is turned on When the line power switch stands out and the green light
72. e standard seals They cause extremely high abrasion and significantly shorten seal life time For the use with normal phase applications special polyethylene pistons seals yellow color PE seals pack of 2 0905 1420 are available These seals have less abrasion compared to the standard seals The seal wear in procedure causes problems to the normal phase seals yellow They will be destroyed by the procedure gt DO NOT apply the seal wear in procedure performed to normal phase seals 82 1 Remove the standard seals from the pump head Maintenance of a Pump Head without Seal Wash on page 159 2 Install normal phase seals Polyethylene seals have a limited pressure range of 0 200 bar When used above 200 bar their lifetime will be significantly reduced 1260 Infinity Binary Pump User Manual Optimizing Performance 5 When to Use the Low Volume Mixer The Low volume mixer 200 uL 5067 1565 is designed for use with the 1260 Infinity Binary LC System in low delay volume mode This configuration is typically used for 2 1 mm i d 1 8 um particle size columns where emphasis is put on S N ratio The low volume mixer helps mixing gradients starting with a low concentration of organic solvents which can cause noise on the baseline The maximum benefit of the mixer is achieved using the mixer together with FW revisions A 06 06 or higher The low volume mixer is installed with the corresponding holder Low Volume Mixe
73. echanical and thermal stability PEEK is therefore the material of choice for UHPLC and biochemical instrumentation It is stable in a wide pH range and inert to many common solvents There is still a number of known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc 1260 Infinity Binary Pump User Manual Using the Pump 4 Polyimide Agilent uses semi crystalline polyimide for rotor seals in valves and needle seats in autosamplers One supplier of polyimide is DuPont which brands polyimide as Vespel which is also used by Agilent Polyimide is stable in a pH range between 1 and 10 and in most organic solvents It is incompatible with concentrated mineral acids e g sulphuric acid glacial acetic acid DMSO and THF It is also degraded by nucleophilic substances like ammonia e g ammonium salts in basic conditions or acetates Polyethylene PE Agilent uses UHMW ultra high molecular weight PE PTFE blends for yellow piston and wash seals which are used in 1290 Infinity pumps and for normal phase applications in 1260 Infinity pumps Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12 5 It is compatible to many organic solvents used
74. ed Preparations 136 Running the Pump Elasticity Calibration from the Agilent Lab Advisor Software The initial calibration is done at the factory It only needs to be repeated after replacement of major pump parts mainboard pump drive p n Description 8710 0510 Wrench 1 4 5 16 inch open p n Description G1312 67500 Calibration capillary assembly Place all bottle heads in to a bottle of HPLC grade water Make absolutely sure that the pump is very thoroughly flushed with the solvent to be calibrated before starting the procedure Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the calibration to fail If a solvent selection valve is installed flush all four solvent channels to avoid that air from a dry solvent intake tube is drawn into the flow path upon initialization 1 Select the pump elasticity calibration from the test selection menu 2 Start the test and follow the instructions Make sure to release the pressure by opening the purge valve when the test has completed Otherwise the pump may generate an overpressure error 1260 Infinity Binary Pump User Manual Test Functions and Calibration 8 Leak Rate Test Introduction The Leak Rate Test is used for verifying the internal tightness of the pump and helps identifying parts which may have caused a leak System requirements Minimum software revisions Lab Advisor B 02 03 Minimum firmware revisions e A 06
75. ed for low pressure mixing pumps like Agilent quaternary pumps High pressure mixing pumps like Agilent binary pumps are more robust with respect to bubble formation but a degasser is recommended for best performance Additionally a degasser is highly recommended for the following applications Your detector is used with maximum sensitivity in the low UV wavelength range e Your application requires highest injection precision e Your application requires highest retention time reproducibility flow rates below 0 5 mL min The binary pump is used with bypassed damper and mixer Operational Hints for the Vacuum Degasser If you are using the vacuum degasser for the first time if the vacuum degasser was switched off for any length of time for example overnight or if the vacuum degasser chambers are empty you have to prime the vacuum degasser before running an analysis Priming is usually done by pumping at a high flow rate 8 5 mL min Alternatively a syringe can be used to draw the solvent through the empty degasser if the pump does not aspirate the solvent by itself For details see Initial Priming on page 54 For more information see the Agilent 1260 Infinity Standard Degasser User Manual 1260 Infinity Binary Pump User Manual Optimizing Performance 5 When to Use the Active Seal Wash Option Concentrated buffer solutions will reduce the lifetime of the seals and pistons in your binary pump The active seal wa
76. emineralized water which was filtered through about 0 2 um filters e Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle Solvent bottle amber 9301 1450 supplied with the instrument for your aqueous mobile phase e If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase 1260 Infinity Binary Pump User Manual 75 4 Using the Pump Prevent Blocking of Solvent Filters 76 Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module e Use a sterile if possible amber solvent bottle to slow down algae growth e Filter solvents through filters or membranes that remove algae Exchange solvents every two days or refilter e If the application permits add 0 0001 0 001 M sodium azide to the solvent e Place a layer of argon on top of your solvent Avoid exposure of the solvent bottle to direct sunlight Never use the system without solvent filter installed 1260 Infinity Binary Pump User Manual Using the Pump 4 Checking the Solvent Filters The solvent filters are located on the low pressure side
77. en designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets 1260 Infinity Binary Pump User Manual 41 3 Installing the Pump Installation Information on Leak and Waste Handling Figure 12 Leak and waste handling overview typical stack conf
78. entifying Cables Overview 202 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 35900 60750 01046 60105 Remote cables p n 03394 60600 03396 61010 5061 3378 01046 60201 BCD cables p n 03396 60560 G1351 81600 Description Agilent module to 3394 6 integrators Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description Agilent module to 3396A Series integrators 3396 Series Il 3395A integrator see details in section Remote Cables on page 206 Agilent module to 3396 Series III 3395B integrators Remote Cable Agilent module to general purpose Description Agilent module to 3396 integrators Agilent module to general purpose 1260 Infinity Binary Pump User Manual Identifying Cables 12 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable Agilent module interface board to general purposes RS 232 cables p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5
79. enzione Advertencia Disconnect power cord before moving D branchez le cordon secteur avant tout d placement Entfernen Sie das Netzkabel bevor Sie das Gerat bewegen Scollegare il cavo di alimentazione prima di spostare lo strumento Desconectar el aparato de la red antes de moverlo L 2J I Figure 13 Warning label illustration for correct waste tubing 44 1260 Infinity Binary Pump User Manual Installing the Pump 3 Installing the Binary Pump Parts required Preparations p n Description 1 Pump 1 Data System and or 1 G4208A Instant Pilot 1 Power cord For other cables see text below and Overview on page 202 Locate bench space Provide power connections Unpack the module Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service offic
80. er Manual Maintenance 10 Exchanging the Active Inlet Valve AIV or its Cartridge 4 Using a 14 mm wrench loosen the active inlet valve and remove the valve from the pump head Connector Solvent intake tubing Active inlet valve 5 Using a pair of tweezers remove the valve cartridge from the defective active inlet valve 1260 Infinity Binary Pump User Manual 171 10 Maintenance 6 Active inlet valve cartridge Active inlet valve 10 11 Push the cartridge into the new active inlet valve Screw the new valve into the pump head With the 14 mm wrench turn the nut until it is hand tight Position the valve so that the solvent inlet tube connection points towards the front Using the 14 mm wrench tighten the nut by turning the valve in its final position not more than a quarter turn Do not overtighten the valve Reconnect the Active Inlet Valve cable to the connector in the Z panel and the inlet tube to the valve Reinstall the front cover After an exchange of the valve it may be required to pump several mL of the solvent used in the current application before the flow stabilizes at a pressure ripple as low as it used to be when the system was still working properly 172 1260 Infinity Binary Pump User Manual Maintenance 10 Exchanging the Outlet Valve When if leaking internally Tools required Description Wrench 1 4 1 5 inch Wrench 1 4 inch Wrench 14mm Parts required p n Descri
81. er is greater pumping degassed H30 at 10 MPa 100 bar Operating range 0 60 MPa 0 600 bar 0 8700 psi up to 5 mL min lt 2 amplitude typically lt 1 3 or lt 0 3 MPa 3 bar whatever is greater at 1 mL min isopropanol at all pressures gt 1 MPa 10 bar 147 psi Low delay volume configuration lt 5 amplitude typically lt 2 Pre defined based on mobile phase compressibility 1 0 12 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel High pressure binary mixing measured with water at 1 mL min water caffeine tracer Standard delay volume configuration 600 800 uL includes 400 uL mixer dependent on back pressure Low delay volume configuration 120 pL 1260 Infinity Binary Pump User Manual Table 2 Site Requirements and Specifications 2 Performance Specifications of the Agilent 1260 Infinity Binary Pump G1312B Type Specification Comments Composition range Composition precision Composition accuracy Control Local control Analog output Communications Safety and maintenance GLP features Housing settable range 0 100 recommended range 1 99 or 5 pL min per channel whatever is greater lt 0 15 RSD or lt 0 04 min SD whatever is greater 0 35 absolute at 2 mL min at 10 MPa 100 bar Agilent control software e g ChemStation EZChrom OL MassHunter Agilent Instant P
82. erformance 26 stack configuration low delay volume with post column cooler 38 low delay volume 37 medium delay volume 35 standard delay volume 33 status indicator 95 stroke length 122 stroke volume 14 17 system tool kit 196 T temperature limit exceeded 115 temperature out of range 114 temperature sensor 105 test functions 92 tests pressure test results 129 pressure test 128 pump elasticity calibration 136 solvent calibration 133 solvent compressibility calibration 134 system pressure test 128 timeout 102 troubleshooting error messages 92 101 status indicators 94 92 1260 Infinity Binary Pump User Manual Index U unpacking the pump 30 V vacuum degasser 10 27 61 61 operational hints 80 when touse 80 valve failed 111 valve inlet valve replacement 153 solvent selection 177 variable reluctance motor 14 variable stroke volume 17 voltage range 25 WwW wash solvent 81 waste electrical and electronic equipment 243 WEEE directive 243 weight 25 wrong pump configuration 112 251 www agilent com In This Book This manual contains technical reference information about the Agilent 1260 Infinity Binary Pump G1312B The manual describes the following introduction e site requirements and specifications e installing the pump using the binary pump e optimizing performance e troubleshooting and diagnostics maintenance e parts and materials for maintenance e identifying cables hardware in
83. ert tool 6 Using the steel side of the insert tool remove the seal wash gasket and the wash seal from the support ring The removed seal will be damaged and cannot be re used Wash seal 7 Using the plastic side of the insert tool press the new wash seal spring pointing upwards into the recess of the support ring Insert too Wash seal Support ring 8 Place a seal wash gasket in the recess of the support ring Put the seal holder on top of the gasket Seal holder Seal wash gasket Support ring 1260 Infinity Binary Pump User Manual 165 10 Maintenance Maintenance of a Pump Head with Seal Wash 9 Place the support rings on the piston housing pistons 10 Insert the pistons and carefully press them into the seals not installed and snap the pump housing and piston housing together Note the correct position of the pin on the support ring Ad Piston l A Pump housing Support ring Piston housing Pa 11 Tighten the lock screw 12 Reinstall the pump head assembly see Reinstalling the i Pump Head Assembly on page 167 Lock screw 166 1260 Infinity Binary Pump User Manual Maintenance 10 Reinstalling the Pump Head Assembly When When reassembling the pump Tools required p n Description 8710 2411 Hexagonal key 3 mm Hexagonal key 4 mm Parts required p n Description 1 79846 65501 Pump head grease 1 Slide the pump head assembly onto the pump d
84. es The system pressure has exceeded the upper pressure limit Probable cause 1 Upper pressure limit set too low 2 Blockage in the flowpath after the damper 3 Defective damper 4 Defective main board 1260 Infinity Binary Pump User Manual Suggested actions Ensure the upper pressure limit is set to a value suitable for the analysis Check for blockage in the flowpath The following components are particularly subject to blockage inline filter frit needle autosampler seat capillary autosampler sample loop autosampler column frits and capillaries with small internal diameters e g 50 um ID Please contact your Agilent service representative Please contact your Agilent service representative 109 7 110 Error Information Pressure Below Lower Limit Error ID 2015 2501 The system pressure has fallen below the lower pressure limit Probable cause 1 Lower pressure limit set too high 2 Air bubbles in the mobile phase 3 Leak 4 Defective damper 5 Defective main board Pressure Signal Missing Error ID 2016 Suggested actions Ensure the lower pressure limit is set to a value suitable for the analysis Make sure that the degasser is in flow path and works correctly Purge the module e Ensure solvent inlet filters are not blocked Inspect the pump head capillaries and fittings for signs of a leak e Purge the module Run a pressure test to determine whe
85. etails on correct installation see Installation Information on Leak and Waste Handling on page 41 1260 Infinity Binary Pump User Manual Using the Pump 4 Hints for Successful Use of the Binary Pump e Place solvent cabinet with the solvent bottles always on top or at a higher level of the pump e When using the binary pump without vacuum degasser shortly degas your solvents by putting the solvent to a suitable container and applying a vacuum pressure for some time If possible apply solvent conditions that will decrease the gas solubility over time for example warming up the solvents The use of a vacuum degasser is mandatory for flow rates below 0 5 mL min and for configurations without damper and mixer When using the binary pump with vacuum degasser flush the degasser with at least 5 mL per channel before operating the pump especially when the pumping system had been turned off for a certain length of time for example overnight and volatile solvent mixtures are used in the channels see Regular Priming on page 56 Prevent blocking of solvent inlet filters never use the pump without solvent inlet filters Growth of algae should be avoided see Prevent Blocking of Solvent Filters on page 76 Check purge valve frit and column frit in regular time intervals A blocked purge valve frit can be identified by black yellow or greenish layers on its surface or by a pressure greater than 10 bar in low delay volu
86. finity Binary Pump User Manual Purge Valve Assembly Item p n 1 G1312 60061 2 01018 22707 3 5067 4728 Parts and Materials for Maintenance 11 Description Purge valve 1260 PTFE frits pack of 5 Seal cap U Figure 30 Purge Valve Assembly 1260 Infinity Binary Pump User Manual 193 11 Parts and Materials for Maintenance Active Inlet Valve Assembly Item p n Description 1 G1312 60025 Active inlet valve body without cartridge 2 G1312 60020 Cartridge for active inlet valve 600 bar SS 2 J Figure 31 Active Inlet Valve Assembly 194 1260 Infinity Binary Pump User Manual Parts and Materials for Maintenance 11 HPLC Starter Kit G4201 68707 HPLC Starter Kit incl 0 17 mm i d cap G4201 68707 p n 9301 1420 3x 9301 1450 01018 22707 5182 0716 5182 0717 5063 6507 2x 5041 2168 2x 5065 9939 Description Solvent bottle transparent Solvent bottle amber PTFE frits pack of 5 Screw Cap Vial 2 mL amber glass write on spot 100 pk Blue screw caps 100 pk Chip Column I D Assy Solvent inlet filter 20 um pore size Capillary Fitting Starter Kit 0 17 mm id HPLC Starter Kit G4202 68707 HPLC Starter Kit incl 0 12 mm i d cap G4202 68707 p n 9301 1420 3x 9301 1450 01018 22707 5182 0716 5182 0717 5063 6507 2x 5041 2168 2x G1316 80003 5065 9937 1260 Infinity Binary Pump User Manual Description Solvent bottle transparent Solvent bottle amber PTFE frits pac
87. finity Binary Pump User Manual 11 1 Introduction to the Binary Pump Instrument and Operation Damper and mixer can be bypassed for lowest delay volume of the binary pump This configuration is recommended for low flow rate applications with steep gradients see the Agilent 1260 Infinity Binary LC System Manual Figure 3 on page 12 illustrates the flow path in low delay volume mode For instructions on how to change between the two configurations see Convert the Binary Pump to Low Delay Volume Mode on page 85 NOTE Bypassing the mixer while the damper remains in line is not a supported configuration and may lead to undesired behavior of the binary pump Mixer ie Damper Purge valve Pump outlet Py Pressure sensor to waste Outlet valve Inlet valve Mixing chamber from solvent lt Seals bottle A _ from solvent bottle B Pistons Pistons Pump head A Pump head B Figure 3 The Hydraulic Path of the Binary Pump with Bypassed Damper and Mixer For pump specifications see Performance Specifications on page 26 12 1260 Infinity Binary Pump User Manual Introduction to the Binary Pump 1 Overview of the Hydraulic Path Overview of the Hydraulic Path The solvent from the bottle in the solvent cabinet enters the pump through an active inlet valve Each side of the binary pump comprises two substantially identical pump units Both pump units comprise a ball screw drive and a
88. formation e appendix Agilent Technologies 2005 2008 2010 2013 Printed in Germany 11 2013 G1312 90015 Rev B fe Agilent Technologies
89. gt Do not connect the power cable to the instrument while the covers are removed CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 22 1260 Infinity Binary Pump User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regu
90. hamber each piston stroke of the pump will generate a small pressure pulsation influencing the flow ripple of the pump The amplitude of the pressure pulsation mainly depends on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes generate less pressure pulsation than larger stroke volumes at the same flow rate In addition the frequency of the pressure pulsation will be higher This will decrease the influence of flow pulsations on retention times In gradient mode a smaller stroke volume results in less flow ripple and reduces the composition ripple The binary pump uses a processor controlled ball screw system for driving its pistons The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume The stroke volume for the pump is by default set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended 1260 Infinity Binary Pump User Manual 17 1 Introduction to the Binary Pump Leak and Waste Handling Leak and Waste Handling The 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed Figure 5 Leak and waste handling concept overview typical stack configura
91. he compressibility of the solvents in use The binary pump comes with compressibility parameters for the most common HPLC solvents and solvent mixtures If a solvent is not available in the list of pre calibrated solvents the solvent compressibility calibration allows the appropriate compressibility data to be generated Technical background The solvent compressibility calibration relies on an accurate elasticity calibration of the pump With a proper elasticity calibration in place the pump is switched into pressure control mode A restriction capillary is connected to the purge valve outlet By varying the flow rate the pump maintains a certain pressure The pump optimizes the compressibility value of the solvent until the lowest possible pump ripple is reached The pump increases the flow rate and adjusts the pressure to the next calibration step where the pump ripple is minimized again This process is repeated until solvent compressibility data for the whole operating pressure range of the pump are available The compressibility data set for this solvent is stored in an XML file in C Documents and Settings lt username gt Application Data Agilent Technologies Agilent Lab Advisor 2 02 0 0 data It can be shared with other G1312B pumps via the controlling data system 1260 Infinity Binary Pump User Manual 133 8 Test Functions and Calibration Running the Solvent Compressibility Calibration When Tools required Parts require
92. he main power switch 1 Lift solvent bottles out of the solvent cabinet and place them on the table Disconnect the solvent tubes from the solvent selection valve and empty the tubes into the bottles Place the bottles back into the solvent cabinet 2 Disconnect all tubings from the solvent selection valve 274 J 1260 Infinity Binary Pump User Manual 177 10 Maintenance Exchanging the Solvent Selection Valve 3 Using a Pozidriv screwdriver 1 loosen the holding 4 Carefully pull the valve holder out and disconnect the screws of the valve holder valve cable at the connector 6 Tighten the screws of the valve holder 178 1260 Infinity Binary Pump User Manual Maintenance 10 Exchanging the Solvent Selection Valve 7 Reconnect all tubings to the solvent selection valve A F A gt ra NOTE After an exchange of the valve it may be required to pump several mL of solvent before the flow stabilizes at a pressure ripple as low as it used to be when the system was still working properly 1260 Infinity Binary Pump User Manual 179 10 Maintenance Exchanging the Optional Interface Board When Board defective Parts required Description 1 BCD Interface board Preparations Switch OFF the module at the main power switch Unplug the module from main power CAUTION Electronic boards and components are sensitive to electrostatic discharge ES
93. his elasticity pressure function is known a correction algorithm can be applied This results in significantly improved pump performance in low delay volume mode damper and mixer bypassed The pump elasticity calibration uses a solvent with well known properties HPLC grade water to determine the pump elasticity over the entire operating pressure range and stores the calibration values in the non volatile RAM of the pump mainboard The initial calibration is done at the factory It only needs to be repeated after replacement of major pump parts mainboard pump drive The test allows to define which pump head will be calibrated Results of the pump elasticity calibration rely on known compressibility parameters for pure water If the water is not HPLC grade not well degassed or degasser and pump are not flushed properly the pump elasticity calibration will fail The pump elasticity calibration has to be performed for each pump head individually Incorrect pump elasticity calibration Solvent compressibility calibrations acquired with a miscalibrated pump will work but they are not transferable to other pumps A correct pump elasticity calibration is an essential prerequisite for successful solvent compressibility calibrations gt Calibrate the pump elasticity correctly 1260 Infinity Binary Pump User Manual 135 8 Test Functions and Calibration Running the Pump Elasticity Calibration When Tools required Parts requir
94. iguration as an example 42 1260 Infinity Binary Pump User Manual Installing the Pump 3 1 Solvent cabinet Leak pan 3 Leak pan s outlet port A leak funnel B and corrugated waste tube C 4 Waste tube of the sampler s needle wash 5 Condense drain outlet of the autosampler cooler 6 Waste tube of the purge valve 7 Waste tube 1 Stack the modules according to the adequate stack configuration The leak pan outlet of the upper module must be vertically positioned above the leak tray of the lower module see Figure 12 on page 42 2 Connect data and power cables to the modules see section Installing the Module below 3 Connect capillaries and tubes to the modules see section Flow Connections to the module below or the relevant system manual WARNING Toxic flammable and hazardous solvents samples and reagents gt Keep solvent path free from blockages gt Keep the flow path closed in case the pump in the system is equipped with a passive inlet valve solvent may leak out due to hydrostatic pressure even if your instrument is off gt Avoid loops gt Tubes must not sag gt Do not bend tubes gt Do not immerse tube end in waste liquid gt Do not intubate tubes in other tubes gt For correct tubing follow instructions on label attached to the module 1260 Infinity Binary Pump User Manual 43 3 Installing the Pum I 3s Int y Inst lle ion intorn Warning Attention Warnung Att
95. ilot For pressure monitoring 1 33 mV bar one output Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN optional Extensive support for troubleshooting and maintenance is provided by the Instant Pilot Agilent Lab Advisor and the Chromatography Data System Safety related features are leak detection safe leak handling leak output signal for shutdown of pumping system and low voltages in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre defined and user settable limits and feedback messages Electronic records of maintenance and errors All materials are recyclable at 0 2 and 1 mL min based on retention time at constant room temperature water caffeine tracer Revision B 02 00 or above For use with flow rates below 500 ul min or for use without damper and mixer a vacuum degasser is required All specification measurements are done with degassed solvents 1260 Infinity Binary Pump User Manual 27 2 Site Requirements and Specifications Performance Specifications 28 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual 3 Installing the Pump Unpacking the Binary Pump 30 Optimizing the Stack Configuration 33 Installation Information on Leak and Waste Handling 41 Installing the Binary Pump 45 Flow Connections with Sol
96. imple Repairs 152 Maintenance Procedures 153 Exchanging the Purge Valve Frit or the Purge Valve 154 Removing the Pump Head Assembly 157 Maintenance of a Pump Head without Seal Wash 159 Maintenance of a Pump Head with Seal Wash 163 Reinstalling the Pump Head Assembly 167 Seal Wear in Procedure 169 Exchanging the Active Inlet Valve AIV or its Cartridge 170 Exchanging the Outlet Valve 173 Installation of the Solvent Selection Valve Upgrade Kit 175 Exchanging the Solvent Selection Valve 177 Exchanging the Optional Interface Board 180 Replacing Module Firmware 181 1 Parts and Materials for Maintenance 183 Hydraulic Path with Solvent Selection Valve 184 Hydraulic Path without Solvent Selection Valve 186 Pump Head Assembly Without Seal Wash 188 Pump Head Assembly with Seal Wash Option 190 Outlet Valve 192 Purge Valve Assembly 193 Active Inlet Valve Assembly 194 HPLC Starter Kit G4201 68707 195 HPLC Starter Kit G4202 68707 195 HPLC System Tool Kit 196 Bottle Head Assembly 197 Solvent Cabinet 198 1260 Infinity Binary Pump User Manual Contents 12 Identifying Cables 201 Overview 202 Analog cables 204 Remote Cables 206 BCD Cables 209 CAN LAN Cables 211 External Contact Cables 212 RS 232 Cable Kit 213 Agilent 1200 Module to Printer 214 13 Hardware Information 215 Firmware Description 216 Electrical Connections 219 Interfaces 221 Setting the 8 bit Configuration Switch without On board LAN 228 O
97. in Table 8 on page 89 If the solvent in use is not listed in the compressibility table when using premixed solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the compressibility settings 1 Start channel A of the binary pump with the required flow rate 2 Before starting the optimization procedure the flow must be stable Use degassed solvent only Check the tightness of the system with the pressure test see System Pressure Test on page 126 3 Your pump must be connected to an Agilent data system or Instant Pilot the pressure and ripple can be monitored with one of these instruments otherwise connect a signal cable between the pressure output of the isocratic pump and a recording device for example 339X integrator and set following parameters Zero 50 Att 2 3 Chart Speed 10 cm min 4 Start the recording device in plot mode 5 Starting with a compressibility setting of 40 x 10 6 bar increase the value in steps of 10 Re zero the integrator as required The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition 6 Repeat step 1 through step 5 for the B channel of your binary pump 1260 Infinity Binary Pump User Manual Optimizing Performance 5 Table8 Solvent Compressibility Solvent pure Compressibility 10 bar Acetone 126 Acetonitrile 115 Benzene
98. in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated acids and bases For normal phase applications the maximum pressure should be limited to 200 bar Tantalum Ta Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide It can be corroded by strong bases e g hydroxide solutions gt 10 diethylamine It is not recommended for the use with fluoric acid and fluorides Stainless Steel ST Stainless steel is inert against many common solvents It is stable in the presence of acids and bases in a pH range of 1 to 12 5 It can be corroded by acids below pH 2 3 It can also corrode in following solvents Solutions of alkali halides their respective acids for example lithium iodide potassium chloride and so on and aqueous solutions of halogens 1260 Infinity Binary Pump User Manual 71 4 72 Using the Pump High concentrations of inorganic acids like nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCly 02 gt 2 COCl 2 HCl This reaction in which stain
99. inary Pump User Manual 141 8 Test Functions and Calibration Leak Rate Test 142 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual e Diagnostic Signals J Analog Pressure Output 144 Diagnostic Signals in Chromatographic Data Systems Instrument Signals 145 This chapter explains all diagnostic signals of the binary pump i Agilent Technologies 145 143 9 Diagnostic Signals Analog Pressure Output A BNC connector at the rear of the binary pump provides the reading of the pressure sensor as analog value with a resolution of 1 33 mV bar The maximum reading of 660 bar equals 800 mV The signal is available in real time and can be fed into an appropriate recording device e g integrator or strip chart recorder for troubleshooting purposes M O Pe eD e Analog pressure K pos AEA ouipu pr sore ar Pr am n Figure 22 Location of analog output connector 144 1260 Infinity Binary Pump User Manual Diagnostic Signals 9 Diagnostic Signals in Chromatographic Data Systems Instrument Signals In Chromatographic Data Systems for example Agilent OpenLAB CDS or any 3rd party software that supports the ICF Instrument Control Framework standard the following instrument parameters are accessible during data acquisition and can be stored in the data file current pump pressure solvent composition gradient 1260 Infinity Binary Pump User Manual 145 9 Di
100. it the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 1260 Infinity Binary Pump User Manual Error Information 7 Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Ple
101. ition Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built in 103 7 104 Error Information Remote Timeout Error ID 0070 A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause Suggested actions 1 Not ready condition in one of the Ensure the instrument showing the not ready instruments connected to the remote line condition is installed correctly and is set up correctly for analysis 2 Defective remote cable Exchange the remote cable 3 Defective components in the instrument Check the instrument for defects refer to the showing the not ready condition instrument s documentation Lost CAN Partner Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure
102. ive clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 54 The purge tool of the Lab Advisor can be used to purge the pump automatically If the pump is not able to draw in the solvent from the bottles use a syringe to move the solvent manually through tubing and degasser When priming the vacuum degasser with a syringe the solvent is drawn through the degasser tubes very quickly The solvent at the degasser outlet will therefore not be fully degassed Pump for approximately 10 minutes at your desired flow rate before starting an analysis This will allow the vacuum degasser to properly degas the solvent in the degasser tubes 1260 Infinity Binary Pump User Manual a A O N Installing the Pump 3 Open the purge valve of the pump Set the flow rate to 5 mL min Select channel Al Turn the flow on Observe if the solvent in the tubing of channel Al is advancing towards the pump If it isn t disconnect the solvent tubing from the solvent selection valve attach a syringe with a syringe adapter and pull the liquid through the degasser Reattach the tubing to the solvent selection valve Pump 30 mL isopropanol to remove residual air bubbles Switch to the next solvent channel and repeat steps 5 and 6 until all channels have been purged Turn the flow off and close the purge valve 1260 Infinity Binary Pump User Manual 55
103. k of 5 Screw Cap Vial 2 mL amber glass write on spot 100 pk Blue screw caps 100 pk Chip Column I D Assy Solvent inlet filter 20 um pore size Heater long down 0 12 mm i d 1 6 uL internal volume Capillary Fitting Starter Kit 0 12 mm id 195 11 Parts and Materials for Maintenance HPLC System Tool Kit The HPLC System Tool Kit G4203 68708 contains some accessories and tools needed for installation and repair of the module p n Description 0100 1681 Adapter syringe seal wash tube 0100 1710 Mounting Tool for Tubing Connections 01018 23702 Insert tool 5023 0240 Hex driver 14 slitted 8710 0060 Hex key wrench 9 64 inch 8710 0510 2x Wrench open 1 4 5 16 inch 8710 0641 Hex key set 1 5 mm 8710 0899 Pozidriv screwdriver 8710 1534 Wrench 4 mm both ends open end 8710 1924 Wrench open 14 mm 8710 2392 Hex key 4 mm15 cm long T handle 8710 2393 Hex key 1 5 mm straight handle 10 cm 8710 2394 Hex key 9 64 inch 15 cm long T handle 8710 2409 Wrench open end 5 16 3 8 inch 8710 2411 Hex key 3 mm12 cm long 8710 2412 Hex key 2 5 mm 15 cm long straight handle 8710 2438 Hex key 2 0 mm 8710 2509 Screwdriver Torx TX8 8710 2594 Double open end wrench 4 mm 9301 0411 Syringe Plastic 9301 1337 Adapter syringe solvent tube with fitting 196 1260 Infinity Binary Pump User Manual Parts and Materials for Maintenance 11 Bottle Head Assembly Item p n Description 1 9301 1450 Solvent bottle amber 2 9301 1420 S
104. kip steps that deal with the purge valve 1260 Infinity Binary Pump User Manual 163 10 Maintenance Maint ec 1 Place the pump head on a flat surface Loosen the lock screw two turns and while holding the lower half of the assembly piston housing carefully lift up the pump housing and remove it from the piston housing Lock screw Pump housing Piston housing 2 Remove the seal holder and the seal wash support rings from the piston housing Remove the seal holder from the support ring assembly Seal holder Seal wash support ring Piston housing 2 2 Piston 3 Check the piston surface and remove any deposits or layers Most suitable is polishing of the piston rod with toothpaste Replace the piston if scratched or if dents are visible Piston surface The best way to inspect a piston is to hold it up and watch e g a light bulb through the piston rod The transparent sapphire acts as a very strong magnifier and 164 4 Using the steel side of the insert tool carefully remove the seal from the pump housing Remove wear retainers if still present Insert tool Piston seal Pump housing wis even smallest surface abnormalities become visible 1260 Infinity Binary Pump User Manual Maintenance 10 Maintenance of a Pump Head with Seal Wash 5 Using the plastic side of the insert tool insert the new seals into the pump head and press them firmly in position Ins
105. l bottle volume and initial filling volume set correctly the pump subtracts the consumed volume continuously from the initial value and stops the pump and method sequence execution before the system runs dry or an analysis is corrupted The bottle filling feature fails if multiple solvent inlets are put into one solvent bottle gt In that case implement a minimum pressure limit see Table 4 on page 64 to avoid that the pump runs dry when solvents are empty Table 6 on page 68 lists the available bottle filling parameters 1260 Infinity Binary Pump User Manual 67 4 Using the Pump Table6 Bottle Filling Parameters Parameter Limits Description Total 0 1000L This is the capacity maximum possible volume in liter of the solvent bottle In Volume default 0 L combination with the actual volume this parameter is used for calculating and displaying the relative liquid level Actual 0 1000L After filling the solvent bottles enter the actual volumes into these boxes The Volume default 0 L Actual Volume must not be larger than the Total Volume of the bottle Prevent default unchecked If this option is checked the pump won t start a new run if the solvent level in analysis one or more bottles is below the minimum volume Enter a minimum volume in liter which considers the position of the solvent inlet and size shape of the solvent bottle such that no air is drawn if the actual volume gets close to this limit
106. lations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment 1260 Infinity Binary Pump User Manual 23 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 1 on page 25 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 24 1260 Infinity Binary Pump User Manual Physical Specifications Site Requirements and Specifications 2 Table 1 Physical Specifications Type Specification Comments Weight 15 5 kg 34 Ibs Dimensions 180 x 345 x 435 m
107. ld result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1260 Infinity Binary Pump User Manual In This Guide In This Guide This manual covers the Agilent 1260 Infinity Binary Pump G1312B 1 Introduction to the Binary Pump This chapter gives an introduction to the module and an instrument overview 2 Site Requirements and Specifications This chapter provides information about site requirements and specifications for the binary pump 3 Installing the Pump This chapter gives information about the preferred stack setup for your system and the installation of your binary pump 4 Using the Pump This chapter explains the operational parameters of the binary pump 5 Optimizing Performance This chapter gives information on how to optimize the performance of the Binary Pump under special operational conditions 6 Troubleshooting and Diagnostics Overview of the troubleshooting and diagnostic features 7 Error Information This chapter describes the meaning of error messages and provides information on
108. les and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides 1260 Infinity Binary Pump User Manual 149 10 Maintenance CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only
109. less steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Diamond Like Carbon DLC Diamond Like Carbon is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fused silica and Quartz Si0 Fused silica is used in 1290 Infinity Flow Cells and capillaries Quartz is used for classical flow cell windows It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended 1260 Infinity Binary Pump User Manual Using the Pump 4 Gold Gold is inert to all common HPLC solvents acids and
110. m height x width x depth 7 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 220 VA 74 W 253 BTU Maximum Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL 0 55 C 32 131 F 40 70 C 40 158 F lt 95 r h at 40 C 104 F Up to 2000 m 6562 ft Up to 4600 m 15091 ft Installation category II Pollution degree 2 Non condensing For storing the module For indoor use only 1260 Infinity Binary Pump User Manual 25 2 Site Requirements and Specifications Performance Specifications Table 2 Performance Specifications of the Agilent 1260 Infinity Binary Pump G1312B Type Specification Comments Hydraulic system Setable flow range Flow range Flow precision Flow accuracy Pressure operating range Pressure pulsation Compressibility compensation Recommended pH range Gradient formation Delay volume 26 Two dual piston in series pumps with servo controlled variable stroke drive power transmission by gears and ball screws floating pistons Set points 0 001 5 mL min in 0 001 mL min increments 0 05 5 0 mL min lt 0 07 RSD or 0 02 min SD whatever is greater based on retention time at constant room temperature 1 or 10 pL min what ev
111. mbly 193 Active Inlet Valve Assembly 194 HPLC Starter Kit G4201 68707 195 HPLC Starter Kit G4202 68707 195 HPLC System Tool Kit 196 Bottle Head Assembly 197 Solvent Cabinet 198 This chapter lists all parts and tools that are required for maintenance Apg Agilent Technologies 183 11 Parts and Materials for Maintenance Hydraulic Path with Solvent Selection Valve 184 Item 11 13 14 15 16 17 p n G1322 67300 G1312 60068 5041 8365 G1312 60003 G1312 60025 G1312 60045 G1312 60067 G1312 87300 G1312 67302 G1312 87301 G1312 87305 G1312 87330 G1312 87306 G1312 04100 G1312 60061 5042 8507 5065 9978 G1312 87303 G1312 87304 5062 2461 Description Kit of 4 solvent tubes including labels for connection degasser to SSV 1260 Infinity Solvent selection valve includes holder Blank plug for unused SSV channels Connecting tube 1260 Infinity Solvent selection valve to active inlet valve Active inlet valve body without cartridge Pump head assembly with seal wash Outlet valve 1220 1260 Absorber capillary Capillary channel A and B pump head outlet to mixing chamber included Restriction capillary mixing capillary to pressure sensor Capillary SSL 0 17 x 150 mm pressure sensor to damper Mixer Capillary SSL 0 17 x 105 mm connections to solvent mixer Bracket for solvent mixer Purge valve 1260 Peristaltic pump cartridge silicone tubing Tubing 1 mm i d 3 mm o d silicone
112. me configuration and 20 bar in standard configuration when pumping distilled water at a rate of 5 mL min with an open purge valve e Whenever possible use a minimum flow rate of 5 uL min per solvent channel to avoid crossflow of solvent into the unused pump channel e Whenever exchanging the pump seals the purge valve frit should be exchanged too e When using buffer solutions flush the system with water before switching it off The seal wash option should be used when buffer solutions with concentrations of 0 1 M or higher are being pumped for long periods of time e Check the pump pistons for scratches grooves and dents when changing the piston seals Damaged pistons cause micro leaks and will decrease the lifetime of the seals e After changing the piston seals apply the seal wear in procedure see Seal Wear in Procedure on page 169 Place the aqueous solvent on channel A and the organic solvent on channel B The default compressibility settings are set accordingly 1260 Infinity Binary Pump User Manual 61 4 Using the Pump Setting up the Pump with the G4208A Instant Pilot Generic operation of the G4208A Instant Pilot is covered in the Agilent Instant Pilot G4208A User s Guide G4208 90006 Details about setting up module specific parameters can be found in the Instant Pilot online help The pump parameters are described in detail in Overview on page 63 62 1260 Infinity Binary Pump User Manual Using the Pu
113. mentation 234 1260 Infinity Binary Pump User Manual Hardware Information 13 The following cards can be used with the Agilent 1260 Infinity modules Table 25 LAN Boards Type Vendor Supported networks Interface board LAN G1369B Agilent Technologies Fast Ethernet Ethernet 802 3 or G1369 60002 or RJ 45 10 100Base TX Interface board LAN G1369C recommended for re ordering or G1369 60012 LAN Communication Interface Agilent Technologies Fast Ethernet Ethernet 802 3 board G1369A or RJ 45 10 100Base TX G1369 60001 obsolete J4106A Hewlett Packard Ethernet 802 3 RJ 45 10Base T J4105A Hewlett Packard Token Ring 802 5 DB9 RJ 45 10Base T J4100A Hewlett Packard Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX BNC 10Base2 1 These cards may be no longer orderable Minimum firmware of these Hewlett Packard JetDirect cards is A 05 05 Recommended LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection 1260 Infinity Binary Pump User Manual 235 13 Hardware Information The Solvent Selection Valve SSV 236 The solvent selection valve is available optionally and as an upgrade It allows using four different solvents for binary pumps The available channels are Al A2 and B1 B2 The valve can switch between positions 1 and 2 i e two different solvent
114. mp 4 Setting up the Agilent 1260 Infinity Binary Pump G1312B with the Instrument Control Interface Overview Parameters described in following sections is offered by the instrument control interface and can usually be accessed through Agilent instrument control software For details please refer to manuals and online help of respective user interfaces 1260 Infinity Binary Pump User Manual 63 4 Using the Pump Setup of Basic Pump Parameters The most important parameters of the pump are listed in Table 4 on page 64 Table4 Basic pump parameters Parameter Limits Description Flow 0 001 5 mL min Stop Time 0 01 min no limit e PostTime off 99999 min e Pressure Max 0 600 bar Limits Min 0 600 bar e SolventA 0 100 SolventB off 100 Solvent H20 ACN MeOH IPA type Solvent Comment Timetable max number of lines depends on free space in pump Total flow rate of the pump See When to Remove Damper and Mixer on page 84 for pump hardware modifications to achieve lowest delay volume The stop time of the pump usually controls the run time of the whole LC system Use no limit to stop the run manually useful for method development Time between the end of a run and the start of the next Used for column equilibration after a gradient Max must be bigger than Min Set max pressure to the maximum operating pressure of your column A min pressure setting of e g 10 bar
115. n page 135 Leak Rate Test The Leak Rate Test is a diagnostic test designed to determine the pressure tightness of the pump components When a problem with the pump is suspected use this test to help troubleshoot the pump and its pumping performance see Leak Rate Test on page 137 Diagnostic Signals The pump has several signals pressure voltages and piston movement that can be used for diagnosing pressure stability composition and flow problems see chapter Diagnostic Signals 1260 Infinity Binary Pump User Manual 93 6 Troubleshooting and Diagnostics Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status Indicator Power switch i Serial number Figure 21 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 94 1260 Infinity Binary Pump User Manual Troubleshooting and Diagnostics 6 Module Status Indicator The module status indicator indicates one of six possible module conditions When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator in
116. nel A B Index Limit Pump channel B The time required by the piston to reach the encoder index position was too short pump During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is reached too fast the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative 120 1260 Infinity Binary Pump User Manual Error Information 7 Index Adjustment Error ID 2204 2214 Index Adjustment Pump channel A B Index Adjustment Pump channel B The encoder index position in the module is out of adjustment During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the time to reach the index position is too long the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective
117. nfiguring your instrument refer to the Agilent 1260 Infinity Binary LC System User Guide 38 1260 Infinity Binary Pump User Manual Installing the Pump 3 Optimizing the Stack Configuration Degasser Pump 400 mm x 0 17 mm HiP AL 012 a seat ca 320 mm x 0 12 mm Cooler for ALS New LowDelayVolume Heat Exchanger New LDV post column cooler TCC 0 12 mm ID cap 2 pl cell DAD Figure 10 Agilent 1260 Infinity Binary LC in low delay volume configuration for 2 1 mm and 3 0 mm id columns 1260 Infinity Binary Pump User Manual 39 3 Installing the Pump Optimizing the Stack Configuration Agilent 1260 Infinity Binary LC in Low Delay Volume Configuration with Automated Column Regeneration and MS Degasser Reg Pump Pump 40 This is the recommended setup to achieve minimum cycle time using MS detection For a more detailed help on configuring your instrument refer to the Agilent 1260 Infinity Binary LC System User Guide DAD or MWD 2 ul cell New LDV post column cooler 0 12 mm ID cap TCC SQ MSD WPS 0 12 mm seat N Cooler f WPS A 650 mm x 0 17 mm ca 320 mm x 0 12 mm Figure 11 Agilent 1260 Infinity Binary LC with Automated column regeneration and MS detection in low delay volume configuration 1260 Infinity Binary Pump User Manual Installing the Pump 3 Installation Information on Leak and Waste Handling The Agilent 1200 Infinity Series has be
118. nfinity Binary Pump User Manual 111 7 Error Information Missing Pressure Reading Error ID 2054 The pressure readings read by the pump ADC analog digital converter are missing The ADC reads the pressure signal of from the damper every lms If the readings are missing for longer than 10 s the error message is generated Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Wrong Pump Configuration Error ID 2060 At switch on the pump has recognized a new pump configuration The binary pump is assigned its configuration at the factory If the active inlet valve and pump encoder of channel B are disconnected and the binary pump is rebooted the error message is generated Probable cause Suggested actions 1 Active inlet valve and pump encoder of Please contact your Agilent service channel B disconnected representative 112 1260 Infinity Binary Pump User Manual Error Information 7 Electronic Fuse of SSV Open Error ID 2049 Valve Fuse 0 Channels Al and A2 Valve Fuse 1 Channels B1 and B2 One of the solvent selection valves in the module has drawn excessive current causing the selection valve electronic fuse to open Probable cause Suggested actions 1 Defective solvent selection valve Restart the pump If
119. ng the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up 1260 Infinity Binary Pump User Manual 229 13 Hardware Information 230 Table 19 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 20 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 21 Parity Settings without on board LAN Switches Parity 7 8 0 0 No Parity 0 1 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity 1260 Infinity Binary Pump User Manual Hardware Information 13 Special Settings The special settings are required for specific actions normally in a service case Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilitie
120. nown and documented pure water When pumping water and using its property data for controlling the pump any deviations from the theoretical pressure profile during solvent recompression are caused by the elasticity of the pump The Pump Elasticity Calibration calculates correction factors to compensate for the individual elasticity of the pump that is being calibrated The elasticity is different for every pump and may change with the replacement of parts in the flow path e g pump seals All binary pumps are elasticity calibrated at the factory and require recalibration only after preventive maintenance or major repairs to the flow path Replacement of capillaries or PTFE frits are not considered as a major repair Incorrect pump elasticity calibration Solvent compressibility calibrations acquired with a miscalibrated pump will work but they are not transferable to other pumps A correct pump elasticity calibration is an essential prerequisite for successful solvent compressibility calibrations gt Calibrate the pump elasticity correctly 1260 Infinity Binary Pump User Manual 15 1 16 Introduction to the Binary Pump What is Solvent Compressibility Compensation Although the compressibility of liquids is orders of magnitude lower than the compressibility of gases without correction a noticeable volume error would be seen if typical chromatographic solvents are compressed to operating pressures as high as 600 bar In additi
121. o form binary mixtures isocratic or gradient from one of two solvents per channel Active seal wash optional is available for use with concentrated buffer solutions Outlet valve Purge valve Active inlet valve Figure 1 Overview of the binary pump 10 1260 Infinity Binary Pump User Manual Introduction to the Binary Pump 1 Instrument and Uperation Principle of Operation The binary pump is based on a two channel dual piston in series design which comprises all essential functions that a solvent delivery system has to fulfill Metering of solvent and delivery to the high pressure side are performed by two pump assemblies which can generate pressure up to 600 bar Each channel comprises a pump assembly including pump drive pump head active inlet valve with replaceable cartridge and outlet valve The two channels are fed into a low volume mixing chamber which is connected via a restriction capillary coil to a damping unit and a mixer A pressure sensor monitors the pump pressure A purge valve with integrated PTFE frit is fitted to the pump outlet for convenient priming of the pumping system Purge valve Pump outlet 4 Pressure sensor to waste Outlet Outlet Inlet valve Mixing from chamber n from solvent l Seals solvent bottle A E Soie a a E bottle B Pistons Pistons Pump head A Pump head B Figure2 The Hydraulic Path of the Binary Pump with Damper and Mixer 1260 In
122. odule Configuration switch Slot for interface board Analog output CAN Bus SEE git Technelegies ceme gy Power plug Figure 36 Electrical connections to the binary pump 220 1260 Infinity Binary Pump User Manual Hardware Information 13 Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table 14 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Pumps G1310B Iso Pump 2 Yes No Yes 1 Yes G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes Yes No Yes CAN DC OUT for CAN G4204A Quat Pump slaves G1361A Prep Pump 2 Yes No Yes No Yes CAN DC OUT for CAN slaves Samplers G1329B ALS 2 Yes No Yes No Yes THERMOSTAT for G2260A Prep ALS G1330B K1330B G1364B FC PS 2 Yes No Yes No Yes THERMOSTAT for G1364C FC AS G1330B K1330B G1364D FC uS CAN DC OUT for CAN G1367E HiP ALS slaves K1367E HiP ALS Clinical Ed G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler G4226A ALS 2 Yes No Yes No Yes 1260 Infinity Binary Pump User Manual 221 13 Hardware Information Table 14 Agilent 1200 Infinity Series Interfaces
123. oes not re initialize CAUTION Upon initialization the pump ignores the Maximum Flow Gradient value This can result in a rapid and uncontrolled pressure increase gt To prevent harm to the column open the purge valve until the initialization is finished The optional seal wash pump can be controlled by either switching it off using it for a single time or specifying frequency and duration of periodic wash intervals 1260 Infinity Binary Pump User Manual 65 CAUTION Using the Pump Auxiliary Pump Parameters The auxiliary pump parameters are pre set to fit most applications Adjustments should only be made when required Table 5 on page 66 shows the available auxiliary parameters with their default values Upon initialization the pump ignores the Maximum Flow Gradient value This can result in a rapid and uncontrolled pressure increase gt To prevent harm to the column open the purge valve until the initialization is finished Table 5 Auxiliary pump parameters Parameter Limits Description Maximum Flow Gradient Minimum Stroke Compressibility 0 1 100 mL min default 100 mL min 20 100 pL default Auto 0 150 10 8 bar bar or enhanced compressibility calibration default use enhanced comp calibration With this parameter flow rate changes can be ramped up and down slowly to avoid pressure shocks to the column The default value is 100 mL min which in fact
124. olvent bottle transparent 3 G1311 60003 Bottle head assembly 4 5063 6598 Ferrules with lock ring 10 Pk 5 5063 6599 Tube screw 10 Pk 6 5062 2483 Solvent tubing 5 m 7 5062 8517 Inlet filter adapter 4 Pk 8 5041 2168 Solvent inlet filter 20 um pore size 6 N 4 8s N sf 11 2 7 y Cary p Figure 32 Bottle Head Assembly Parts 1260 Infinity Binary Pump User Manual 197 11 Parts and Materials for Maintenance Solvent Cabinet Solvent Cabinet Figure 33 Solvent Cabinet Parts 1 Figure 34 Solvent Cabinet Parts 2 198 1260 Infinity Binary Pump User Manual Parts and Materials for Maintenance 11 Item p n Description 1 5065 9981 Solvent cabinet 1200 Infinity including all plastic parts 5043 0207 Name plate 1260 3 5065 9954 Front panel solvent cabinet 4 5042 8907 Leak panel 5 9301 1450 Solvent bottle amber 6 9301 1420 Solvent bottle transparent 7 G1311 60003 Bottle head assembly 1260 Infinity Binary Pump User Manual 199 11 Parts and Materials for Maintenance Solvent Cabinet 200 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual 12 Identifying Cables Overview 202 Analog cables 204 Remote Cables 206 BCD Cables 209 CAN LAN Cables 211 External Contact Cables 212 RS 232 Cable Kit 213 Agilent 1200 Module to Printer 214 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules ot Agilent Technologies 201 12 Id
125. on the compressibility depends on pressure temperature and the amount of dissolved gas In order to minimize the influence of the latter the use of a vacuum degasser is mandatory for a high flow and composition precision Unfortunately the influence of the temperature on compressibility is non linear and cannot be calculated The Agilent 1260 Infinity Binary Pump features a multi point compressibility calibration The compressibility of a solvent is determined at different pressures from 0 600 bar and stored in an XML file This file can be distributed to other pumps because the solvent compressibility is independent from the pump The binary pump and ChemStation come with predetermined solvent compressibility data for the most common HPLC solvents like water acetonitrile methanol etc Users can calibrate their own solvent mixtures with the help of an easy to use calibration procedure in the Agilent Lab Advisor software Let us use the practical example from the last section once again to understand how compressibility compensation works Piston 1 draws solvent at ambient pressure The movement direction is reversed and piston 1 now compresses the solvent until the operating pressure of the HPLC system is reached The outlet valve opens and solvent is pumped by piston 1 into pump chamber 2 Without any compensation the delivered volume at operating pressure would be too low In addition it would take a noticeable amount of time to
126. on acids bases and solvents There are no documented incompatibilities for HPLC applications 1260 Infinity Binary Pump User Manual 73 4 Using the Pump Algae Growth in HPLC Systems 74 The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing e Blocked solvent filters or deposits on inlet or outlet valves resulting in unstable flow composition or gradient problems or a complete failure of the pump e Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure PTFE frits blockage leading to increased system pressure e Column filters to plug giving high system pressure e Flow cell windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the flow path this problem is less common 1260 Infinity Binary Pump User Manual Using the Pump 4 How to Prevent and or Reduce the Algae Problem e Always use freshly prepared solvents especially use d
127. on housing a SS N Reset the seal wear counter and liquimeter in the Agilent Lab Advisor 9 Tighten the lock screw Next Steps 10 Reinstall the pump head assembly see Reinstalling the eN lt S N Lock screw i 4 9 Pump Head Assembly on page 167 SAL O 11 Ifa standard seal has been installed run the seal wear in 1 A if As procedure see Seal Wear in Procedure on page 169 12 For the normal phase seal the purge valve frit should be replaced see Exchanging the Purge Valve Frit or the Purge Valve on page 154 162 1260 Infinity Binary Pump User Manual Maintenance 10 Maintenance of a Pump Head with Seal Wash When Tools required Parts required Preparations In case of maintenance or pump head internal leaks p n Description 8710 2411 Hex key 3 mm12 cm long 8710 2392 Hex key 4 mm15 cm long T handle 01018 23702 Insert tool Screwdriver small flat head p n Description 0905 1175 Wash seal PTFE 5062 2484 Gasket seal wash pack of 6 5063 6586 Sapphire piston Switch off the pump at the main power switch Remove the front cover to have access to the pump heads Removing the Pump Head Assembly on page 157 Both pump head assemblies use the same internal components In addition pump head A is fitted with the purge valve The following procedure describes the removal and disassembly of pump head A left For pump head B right proceed in the same way and s
128. onnections without Solvent Selection Valve Parts required p n Description 1 Other modules 1 G1311 68755 Accessory Kit 2 wrenches 1 4 5 16 inch for capillary connections Preparations Pump is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can carry health risks gt Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Remove the front cover by pressing the snap fasteners on both sides Figure 18 Removing the Front Cover 1260 Infinity Binary Pump User Manual 51 3 52 Installing the Pump Place the solvent cabinet on top of the module Place the bottles into the solvent cabinet and place a bottle head assembly into each bottle Connect the solvent tubes from the bottle head assemblies to the inlet adapters of the active inlet valves Fix the tubes in the clips of solvent cabinet and binary pump Hold the waste tubing with a piece of sandpaper and push it onto the purge valve outlet Place the end into your waste system If the pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the waste tube to the waste outlet of the pump leak handling system Connect
129. or and the module will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the module is switched off and the error message is generated Probable cause Suggested actions 1 Flow path blockage in front of the damper Ensure the capillaries and frits between the pump head and damper inlet are free from blockage 2 Blocked active inlet valve Exchange the active inlet valve 3 Blocked outlet valve Exchange the outlet valve 4 High friction partial mechanical blockage Remove the pump head assembly Ensure there in the pump drive assembly is no mechanical blockage of the pump head assembly or pump drive assembly 5 Defective pump drive assembly Please contact your Agilent service representative 6 Defective main board Please contact your Agilent service representative 7 Restriction capillary blocked at pre mixing Exchange restriction capillary union 116 1260 Infinity Binary Pump User Manual Error Information 7 Encoder Missing Error ID 2046 2050 2510 Encoder Missing Pump channel A B Encoder Missing Pump channel B The optical encoder on the pump motor in the module is missing or defective The processor checks the presence of the pump encoder connector every 2 s If the connector is not detected by the processor the error message is generated Probable cause Suggested actions 1 Defective or disconnected pump encoder Please contact your Agilent
130. orming run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces There is no special interface for this module 1260 Infinity Binary Pump User Manual 227 13 Hardware Information Setting the 8 bit Configuration Switch without On board LAN 228 The 8 bit configuration switch is located at the rear of the module This module does not have its own on board LAN interface It can be controlled through the LAN interface of another module and a CAN connection to that module i SS Y Figure 38 Configuration switch settings depend on configured mode All modules without on board LAN default should be ALL DIPS DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 DIP 1 DOWN and DIP 2 UP allows special RS 232 settings
131. p cartridge silicone tubing Tubing 1 mm i d 3 mm o d silicone 5 m for seal wash option 1260 Infinity Binary Pump User Manual Parts and Materials for Maintenance 11 Hydraulic Path without Solvent Selection Valve 1013 12 16 15 11 Figure 26 Hydraulic Path without Solvent Selection Valve with Active Seal Wash 1260 Infinity Binary Pump User Manual 187 11 Parts and Materials for Maintenance Pump Head Assembly Without Seal Wash Item 10 11 12 13 14a 14b 15 16 188 p n G1312 60056 5063 6586 G1311 60002 5067 1560 5062 2484 5042 8952 G1312 87300 5063 6589 G1311 25200 0515 0175 G1312 23200 G1312 60061 G1312 60067 5042 1303 G1312 60025 G1312 60020 G1312 23201 0515 2118 Description Pump Head 1200 SL without Seal Wash Sapphire piston Piston housing Support Ring SL no seal wash Gasket seal wash pack of 6 Seal holder Absorber capillary Piston seal PTFE carbon filled black pack of 2 default Pump chamber housing Mounting screw for manual purge valve holder M4 20 mm long Holder for manual purge valve Purge valve 1260 Outlet valve 1220 1260 Lock screw Active inlet valve body without cartridge Cartridge for active inlet valve 600 bar Adapter Pump head screw M5 60 mm 1260 Infinity Binary Pump User Manual Parts and Materials for Maintenance 11 Pump Head Assembly Without Seal Wash Figure 27 Pump Head Assembly Without Seal Wash 1260 Infinity Binar
132. p seals 153 purge valve frit 153 purge valve 153 solvent selection valve 153 177 wash seals 153 external contact cable 212 external contacts BCD board 232 F fan failed 107 filter solvent inlet 61 firmware description 216 main system 216 resident system 216 update tool 217 updates 217 181 181 upgade downgrade 181 upgrade downgrade 181 flow accuracy 26 flow connections 48 51 flow precision 26 flow range 10 operational 26 setable 26 flow rate minimum 61 1260 Infinity Binary Pump User Manual Index frequency range 25 G gas solubility 61 gradient formation 26 high pressure mixing 10 hints for successful use 61 HP JetDirect card 234 humidity 25 hydraulic path with solvent selection valve 184 hydraulic path without solvent selection valve 186 hydraulic system 26 l index adjustment 121 index limit 120 index missing 122 initialization failed 123 initialization 14 injection precision 80 inlet valve body 153 inlet valve missing 117 installation bench space 24 instrument layout 238 interface board 180 interface cable 47 interfaces 221 internet 247 introduction to the pump 10 L LAN cable 211 249 Index communication interface board 234 leak rate test evaluating results 140 potential causes of failure 141 running the test 139 eak sensor open 105 eak sensor short 106 eak 105 ine frequency 25 ine voltage 25 iquimeter 162 ithium batteries 244 ost CAN partner 104
133. ption G1312 60067 Outlet valve 1220 1260 Preparations Switch off the pump at the main power switch 1 Using a inch wrench disconnect the absorber capillary from the outlet valve 2 Unscrew the valve with the 14 mm wrench and remove it from the pump body 3 Do not disassemble the outlet valve as this can damage the valve 4 Reinstall the outlet valve and tighten it using a torque wrench 12 Nm 1260 Infinity Binary Pump User Manual 173 10 Maintenance Exchanging the Outlet Valve 5 Reconnect the capillary Outlet valve 174 1260 Infinity Binary Pump User Manual Maintenance 10 Installation of the Solvent Selection Valve Upgrade Kit A solvent selection valve allows you to choose between four different solvents that can be used with a binary pump The valve switches between two solvents Al and A2 for channel A of the left pump head and two solvents B1 and B2 for channel B of the right pump head When Applicable modules This kit is compatible to the 1260 Infinity Binary Pumps G1312B and G1312C and to the 1260 Infinity Binary Pump Clinical ed K1312B Tools required Description Screwdriver Pozidriv 1 Parts required p n Description G1381 60000 Solvent Selection Valve Upgrade Kit Preparations If required remove solvent tubes from the inlet valves The figures below show a Binary Pump G1312B The kit can be used similarly for the Binary Pump G1312C and for the Binary Pump Clinical ed K1312B 1260 Infinity Binary
134. ptional Interface Boards 232 The Solvent Selection Valve SSV 236 Early Maintenance Feedback 237 Instrument Layout 238 14 Appendix 239 General Safety Information 240 The Waste Electrical and Electronic Equipment Directive 243 Lithium Batteries Information 244 Radio Interference 245 Sound Emission 246 Agilent Technologies on the Internet 247 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual e introduction to the Binary Pump e Instrument and Operation 10 Introduction tothe Pump 10 Principle of Operation 11 Overview of the Hydraulic Path 13 Leak and Waste Handling 18 This chapter gives an introduction to the module and an instrument overview ee Agilent Technologies 1 Introduction to the Binary Pump Instrument and Operation Introduction to the Pump The binary pump comprises two identical pumps integrated into one housing Binary gradients are created by high pressure mixing An optional degasser is available for applications that require best flow stability especially at low flow rates for maximum detector sensitivity Pulse damper and mixer can be bypassed for low flowrate applications or whenever a minimal transient volume is desirable Typical applications are high throughput methods with fast gradients on high resolution 2 1 mm columns The pump is capable of delivering flow in the range of 0 1 5 mL min against up to 600 bar A solvent selection valve optional allows t
135. pump drive assembly Please contact your Agilent service representative 1260 Infinity Binary Pump User Manual 121 7 122 Error Information Index Missing Error ID 2205 2215 2505 Index Missing Pump channel A B Index Missing Pump channel B The encoder index position in the module was not found during initialization During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is not recognized within a defined time the error message is generated Probable cause Suggested actions 1 Disconnected or defective encoder cable Please contact your Agilent service representative 2 Defective pump drive assembly Please contact your Agilent service representative Stroke Length Error ID 2206 2216 Stroke Length Pump channel A B Stroke Length Pump channel B The distance between the lower piston position and the upper mechanical stop is out of limits pump During initialization the module monitors the drive current If the piston reaches the upper mechanical stop position before expected the motor current increases as the module attempts to drive the piston beyond the mechanical stop This current increase causes the error message to be generated Probable cause Suggested actions 1 Defective pump drive assembly Please contact your Agilent service representative 1
136. pump head with two sapphire pistons for reciprocating movement To mixing chamber From solvent bottle Ball screw drive Motor with encoder Figure 4 Pump head 1260 Infinity Binary Pump User Manual 13 1 14 Introduction to the Binary Pump A servo controlled variable reluctance motor drives the two ball screw drives in opposite directions The gears for the ball screw drives have different circumferences ratio 2 1 allowing the first piston to move at double the speed of the second piston The solvent enters the pump heads close to the bottom limit and leaves it at its top The outer diameter of the piston is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap in between The first piston has a stroke volume in the range of 20 uL to 100 uL depending on the flow rate The microprocessor controls all flow rates in a range of 1 uL min to 5 mL min The inlet of the first pumping unit is connected to the active inlet valve which is processor controlled opened or closed allowing solvent to be drawn into the first pump unit The outlet of the first pump chamber is connected by a 500 uL absorber capillary to the second pump chamber The outlets of the second chambers of both pump channels joined via a small mixing chamber A coiled restriction capillary connects the mixing chamber via a pressure pulse damper a mixer and a pressure sensor to the purge valve
137. quires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface 1260 Infinity Binary Pump User Manual 101 7 Error Information General Error Messages Timeout Error ID 0062 The timeout threshold was exceeded
138. r Holder G1312 00003 1260 Infinity Binary Pump User Manual 83 5 Optimizing Performance When to Remove Damper and Mixer When to Remove Damper and Mixer The binary pump is equipped with a pressure pulsation damper and a static mixer The total delay volume of the pump is 600 800 uL depending on system pressure The mixer has a volume of 400 uL For applications that require lowest delay volume e g fast gradient methods or gradient applications with low flow rates damper and mixer can be bypassed _ E a Standard configuration Low delay volume configuration Figure 20 Flow Path Modifications of the Binary Pump 84 1260 Infinity Binary Pump User Manual Optimizing Performance 5 Convert the Binary Pump to Low Delay Volume Mode The binary pump is delivered in standard configuration damper and mixer connected This paragraph shows how to bypass damper and mixer and convert the pump to low delay volume mode Configurations where only damper or mixer are disconnected while the other part is still in line are not supported by Agilent Technologies Tools required p n 8710 0510 Description Wrench 1 4 5 16 inch open Wrench 14mm Hex driver open 1 4 inch Preparations Turn the flow off Flush the system water if buffers were used otherwise isopropanol 1 Remove the front cover by pressing the clip fastener on both sides of the cover 2 Use the 1 4 inch hex driver to
139. r any other controlling device connected to your pump 6 Turn OFF the pump slowly open the purge valve to release the pressure from the system disconnect the restriction capillary and reconnect the outlet capillary to the purge valve Reconnect the intake tubing to the solvent selection valve and the connecting tube from the solvent selection valve if installed to the ATV 7 Purge your system with the solvent used for your next application 1260 Infinity Binary Pump User Manual 169 10 Maintenance Exchanging the Active Inlet Valve AIV or its Cartridge When Tools required Parts required Preparations CAUTION If internally leaking backflow Description Wrench 14mm p n Description G1312 60025 Active inlet valve body without cartridge G1312 60020 Cartridge for active inlet valve 600 bar Switch off the pump at the main power switch Ensure correct fit of the active inlet valve Overtightening will destroy the active inlet valve cartridge gt Tighten the active inlet valve properly 170 1 Remove the front cover 2 Unplug the active inlet valve cable from the connector 3 Disconnect the solvent inlet tube at the inlet valve beware of leaking solvents Binary pumps without solvent selection valve SSV have an adapter installed between the solvent line and the active inlet valve AIV Disconnect the solvent tubes at the adapter and remove the adapter from the AIV 1260 Infinity Binary Pump Us
140. remove fitting B from port 2 of the pressure sensor ie Agilent Technologies 1260 Infinity Fi i 1260 Infinity Binary Pump User Manual 5 Optimizing Performance When to Remove Damper and Mixer 3 Fold capillary end B away It remains unconnected Disconnect fitting A from outlet 7 of the mixer 4 Connect fitting A to port 2 of the pressure sensor Seal port 7 of the mixer with a plastic blank nut 1260 Infinity Binary Pump User Manual Optimizing Performance 5 How to Optimize the Compressibility Compensation Setting When a solvent is metered at ambient pressure and compressed to a higher pressure the volume decreases depending on its compressibility Solvent compressibility is a non linear function of pressure and temperature It is specific for each solvent In order to deliver the desired flow accurately at all pressures Agilent pumps use a compressibility compensation For standard LC applications e g using a 400 bar binary pump an average compressibility value for the solvent is sufficient For the 600 bar 1260 Infinity Binary Pump the pressure dependency of a solvent compressibility needs to be considered It is determined at different pressures between 0 600 bar The pump uses the obtained non linear function to select the correct compressibility value for the actual pump pressure Compressibility data for the most common solvents is readily available in the pump firmware
141. rive Purge valve holder P el N I ump head screws 2 Using a 4 mm hexagonal key tighten the pump head screws stepwise with increasing torque 1260 Infinity Binary Pump User Manual 167 10 Maintenance Reinstalling the Pump Head Assembly 3 Reconnect the tubing and capillaries to the connector 168 1260 Infinity Binary Pump User Manual Maintenance 10 Seal Wear in Procedure CAUTION Seal damage This procedure is required for black PTFE seals standard applications p n 5063 6589 but it will damage the yellow PE seals normal phase applications p n 0905 1420 gt Do not run the seal wear in procedure if PE seals are installed in the pumphead 1 Put a bottle with 100 ml of isopropanol in the solvent cabinet and place the solvent intake filter of the pump head you want to wear in into this bottle 2 Screw the PEEK adapter 1 4 28 to 10 32 0100 1847 onto the active inlet valve and connect the inlet tube from the bottle head directly to it 3 Connect the Restriction capillary 5022 2159 to the purge valve Connect its other end to a waste container 4 Open the purge valve and purge the system for 5 min with isopropanol at a flow rate of 2 mL min 5 Close the purge valve set the flow to a value that gives a pressure of 350 bar Pump 15 min at this pressure to wear the seals in The pressure can be monitored on the analog output connector of the pump with the Instant Pilot chromatographic data system o
142. s Table 22 Boot Resident Settings without on board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 0 0 1 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 23 Forced Cold Start Settings without on board LAN Mode Select sw1 sw2 SW3 sSw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 0 0 0 0 0 1 1260 Infinity Binary Pump User Manual 231 13 Hardware Information Optional Interface Boards BCD External Contact Board The Agilent 1200 Infinity Series modules have one optional board slot that allows to add an interface board to the modules Some modules do not have this interface slot Refer to Interfaces on page 221 for details Optional Interface Boards p n Description G1351 68701 Interface board BCD with external contacts and BCD outputs 2110 0004 Fuse for BCD board 250 mA The BCD board provides a BCD output for the bottle number of the Agilent 1200 Series autosampler and four external contacts The external contact closure
143. s are available per channel It is not possible to switch for example between Al and B1 for channel B as solvent paths A and B are completely separated By cascading SSV channels for example using inlets Al and A2 and connecting the outlet of channel A to inlet B1 it is possible to switch between three A1 A2 B2 solvents for one channel here B and using always the same solvent for channel A Technically the 1260 Infinity SSV uses two electromagnetic valves If the current is off inlets A2 and B2 are connected to the outlet If the current is on the valves switch to Al and or Bl This means if the pump is switched off physically solvent connections use inlets A2 and B2 If the pump is in standby for example after using the Off button in ChemStation the SSV retains its previous state Unused channels of an SSV should always be blocked using a plug Blank plug 5041 8365 in order to avoid leaks or air entering the solvent channels 1260 Infinity Binary Pump User Manual Hardware Information 13 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selec
144. s of two independent sections a non instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote e error handling diagnostic functions or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 216 1260 Infinity Binary Pump User Manual Hardware Information 13 Firmware Updates Firmware updates can be done using your user interface PC and Firmware Update Tool with local files on the hard disk e Instant Pilot G4208A with files from a USB Flash Disk Agilent Lab Advisor software B 01 03 and above The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315AB for the G1315A B DAD R the firmware revision for example A for G1315B or B for the G1315C DA
145. sembly i d 6 mm o d 9 mm 1 2 m to waste 5181 1519 CAN cable Agilent module to module 1 m G1329 87300 Capillary ST 0 17 mm x 900 mm S S pump to thermostatted autosampler G1312 87303 Capillary ST 0 17 mm x 400 mm S S pump to injector 5042 9967 Tubing clip set of 5 clips 32 1260 Infinity Binary Pump User Manual Installing the Pump Optimizing the Stack Configuration Agilent 1260 Infinity Binary LC in Standard Delay Volume Configuration This configuration is typically used when using 4 6 mm and 3 0 mm i d columns It is optimized for high flow rates and maximum sensitivity For a more detailed help on configuring your instrument refer to the Agilent 1260 Infinity Binary LC System User Guide 1260 Infinity Binary Pump User Manual 3 33 3 Installing the Pump Optimizing the Stack Configuration Degasser Pump HiP ALS 0 17 mm seat Cooler for ALS TCC DAD 34 400 mm x 0 17 mm 105 mm x 0 17 mm 105 mm x 0 17 mm amp ZeroDeadVolume union 13 ul cell Figure 6 Agilent 1260 Infinity Binary LC in standard delay volume configuration for 4 6 mm and 3 0 mm i d columns 1260 Infinity Binary Pump User Manual Installing the Pump 3 Optimizing the Stack Configuration Agilent 1260 Infinity Binary LC in Medium Delay Volume Configuration This setup is used for best signal to noise ratio using 2 1 mm and 3 0 mm columns For a more detailed help on configuring your instrument refer
146. service connector representative 2 Defective pump drive assembly Please contact your Agilent service representative Inlet Valve Missing Error ID 2048 2052 Inlet Valve Missing Pump channel A B Inlet Valve Missing Pump channel B The active inlet valve in the module is missing or defective The processor checks the presence of the active inlet valve connector every 2 s If the connector is not detected by the processor the error message is generated Probable cause Suggested actions 1 Disconnected or defective cable Ensure the pins of the active inlet valve connector are not damaged Ensure the connector is seated securely 2 Disconnected or defective connection cable Please contact your Agilent service front panel to main board representative 3 Defective active inlet valve Exchange the active inlet valve 1260 Infinity Binary Pump User Manual 117 7 Error Information Servo Restart Failed Error ID 2201 2211 Servo Restart Failed Pump channel A B Servo Restart Failed Pump channel B The pump motor in the module was unable to move into the correct position for restarting When the module is switched on the first step is to switch on the C phase of the variable reluctance motor The rotor should move to one of the C positions The C position is required for the servo to be able to take control of the phase sequencing with the commutator If the rotor is unable to move or if the C position cannot
147. sh option allows to maintain the seal lifetime by flushing the low pressure side of the seals with a wash solvent The seal wash option is strongly recommended if buffer concentrations of 0 1 M or higher are used regularly with the pump The active seal wash option kit can be ordered by quoting Active Seal Wash Upgrade Product including Service G1399A The seal wash option comprises a peristaltic pump secondary seals gaskets seal holders and tubing for both pump heads A bottle of premixed water isopropanol 90 10 vol is placed in the solvent cabinet and connected to the peristaltic pump as described in the technical note that comes with the active seal wash kit Always use a mixture of HPLC grade water 90 and isopropanol 10 as wash solvent This mixture prevents bacteria growth in the wash bottle and reduces the surface tension of the water In order to avoid accumulation of buffer salts or impurities regularly replace the washing solution using fresh solvents The operation of the peristaltic pump can be controlled from the data system or the Instant Pilot For adding a seal wash option please contact your local Agilent Technologies service representative 1260 Infinity Binary Pump User Manual 81 5 Optimizing Performance When to Use Alternative Seals The standard seals for the binary pump can be used for most applications However normal phase applications for example hexane are not compatible with th
148. sure Test on page 126 determined a problem with the pump p n Description 8710 0510 Wrench 1 4 5 16 inch open p n Description G1312 67500 Calibration capillary assembly Place two bottles of HPLC grade water in channels A and B A1 and B1 if the pump is equipped with a solvent selection valve The Valve Test should only be performed when firmware revisions lt A 06 50 are installed For firmware revision A 06 50 or above perform the Leak Rate Test instead Make absolutely sure that the pump is very thoroughly flushed with water before starting the test Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to generate misleading results 1 Select the valve test from the test selection menu 2 Start the test and follow the instructions Make sure to release the pressure by opening the purge valve when the test has completed Otherwise the pump may generate an overpressure error Evaluating the Results 132 Refer to the help file of the Agilent Lab Advisor for further details 1260 Infinity Binary Pump User Manual Test Functions and Calibration 8 Binary Pump Solvent Compressibility Calibration Description Each solvent or solvent mixture has unique compressibility at different pressures In order to deliver accurate flow with minimal pressure and composition ripple over the full operational pressure range it is necessary that the pump compensates precisely for t
149. t simple repairs Replacement of valve cartridges or filters don t require to remove the pump from the system stack Purge valve Outlet valve Active inlet valve Solvent selection valve Pump head Figure 23 Overview of Maintenance and Simple Repairs 1 Purge valve see Exchanging the Purge Valve Frit or the Purge Valve on page 154 2 Outlet valve see Exchanging the Outlet Valve on page 173 3 Active inlet valve see Exchanging the Active Inlet Valve AIV or its Cartridge on page 170 4 Pump head see Removing the Pump Head Assembly on page 157 5 Solvent selection valve see Installation of the Solvent Selection Valve Upgrade Kit on page 175 152 1260 Infinity Binary Pump User Manual Maintenance Procedures Maintenance 10 The procedures described in this section can be done with the binary pump in place in the system stack Table 12 Maintenance procedures Procedure Typical Frequency Notes Exchanging the Purge Valve Frit or the Purge Valve on page 154 Removing the Pump Head Assembly on page 157 Maintenance of a Pump Head without Seal Wash on page 159 Maintenance of a Pump Head with Seal Wash on page 163 Exchanging the Active Inlet Valve AIV or its Cartridge on page 170 Exchanging the Outlet Valve on page 173 Exchanging the Solvent Selection Valve on p
150. t the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the main Please contact your Agilent service board representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative 1260 Infinity Binary Pump User Manual 105 7 106 Error Information Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper lim
151. table limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1260 Infinity Binary Pump User Manual 237 13 Hardware Information Instrument Layout The industrial design of the module incorporates several innovative fea
152. te Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation 1260 Infinity Binary Pump User Manual 181 10 Maintenance 182 Module Specific Information Table 13 Module Specific Information G1312B G1312B Binary pump The module G1312B exists both as part of the 1200 Series and 1260 Infinity product lines These modules use different solvent selection valves and main boards Latest firmware revisions in each set automatically recognize the main board type and can be used without special handling A 06 06 020 A 06 10 020 A 06 34 001 A 06 53 002 These revisions recognize the board type and shall be used A 06 06 is the minimum version which is compatible to 1260 Infinity Binary Pumps G1312B Older G1312B firmware is only compatible to 1200 Series Binary Pumps SL G1312B All listed firmware revisions allow emulation of G1312A pumps Please consult the Firmware Update Guide for 1200 Infinity Systems for details 1260 Infinity Binary Pump User Manual 1260 Infinity Binary Pump User Manual 11 Parts and Materials for Maintenance Hydraulic Path with Solvent Selection Valve 184 Hydraulic Path without Solvent Selection Valve 186 Pump Head Assembly Without Seal Wash 188 Pump Head Assembly with Seal Wash Option 190 Outlet Valve 192 Purge Valve Asse
153. the error message appears again exchange the solvent selection valve 2 Defective connection cable front panel to Please contact your Agilent service main board representative 3 Defective main board Please contact your Agilent service representative AIV Fuse Error ID 2044 Inlet Valve Fuse 0 Pump channel A Inlet Valve Fuse 1 Pump channel B One of the active inlet valves in the module has drawn excessive current causing the inlet valve electronic fuse to open Probable cause Suggested actions 1 Defective active inlet valve Restart the module If the error message appears again exchange the active inlet valve 2 Defective connection cable front panel to Please contact your Agilent service main board representative 3 Defective main board Please contact your Agilent service representative 1260 Infinity Binary Pump User Manual 113 7 Error Information Temperature Out of Range Error ID 2517 Temperature Out of Range 0 Pump channel A Temperature Out of Range 1 Pump channel B One of the temperature sensor readings in the motor drive circuit are out of range The values supplied to the ADC by the hybrid sensors must be between 0 5 V and 4 3 V If the values are outside this range the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 114 1260 Infinity Binary Pump User Manual Error Information 7 Temperature
154. ther the seals or other module components are defective Please contact your Agilent service representative Please contact your Agilent service representative The pressure signal of the damper is missing The pressure signal of the damper must be within a specific voltage range If the pressure signal is missing the processor detects a voltage of approximately 120 mV across the damper connector Probable cause 1 Damper disconnected 2 Defective damper Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative 1260 Infinity Binary Pump User Manual Error Information 7 Valve Failed Error ID 2040 Valve 0 Failed valve Al Valve 1 Failed valve A2 Valve 2 Failed valve B2 Valve 3 Failed valve B1 One of the solvent selection valves in the module failed to switch correctly The processor monitors the valve voltage before and after each switching cycle If the voltages are outside expected limits the error message is generated Probable cause Suggested actions 1 Solvent selection valve disconnected Please contact your Agilent service representative 2 Connection cable inside instrument not Please contact your Agilent service connected representative 3 Connection cable inside instrument Please contact your Agilent service defective representative 4 Solvent selection valve defective Exchange the solvent selection valve 1260 I
155. tion as an example 18 1260 Infinity Binary Pump User Manual Introduction to the Binary Pump 1 The solvent cabinet 1 is designed to store a maximum volume of 6 L solvent The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 5 L For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets a printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet The leak pan 2 individually designed in each module guides solvents to the front of the module The concept covers also leakages on internal parts e g the detector s flow cell The leak sensor in the leak pan stops the running system as soon as the leak detection level is reached The leak pan s outlet port 8 A guides excessive overfill from one module to the next as the solvent flows into the next module s leak funnel 3 B and the connected corrugated waste tube 3 C The corrugated waste tube guides the solvent to the next lower positioned module s leak tray and sensor The waste tube of the sampler s needle wash port 4 guides solvents to waste The condense drain outlet of the autosampler cooler 5 guides condensate to waste The waste tube of the purge valve 6 guides solvents to waste The waste tube connected to the leak pan outlet on each of the bottom instruments 7 guides the solvent to a suitable waste container
156. to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail 1 Select the system pressure test from the test selection menu 2 Start the test and follow the instructions Make sure to release the pressure by opening the purge valve when the test has completed Otherwise the pump may generate an overpressure error 128 1260 Infinity Binary Pump User Manual Test Functions and Calibration 8 Evaluating the Results The sum of all leaks between the pump and the blank nut will add up to the total leak rate Note that small leaks may cause the test to fail but solvent may not be seen leaking from a module Please notice the difference between an error in the test and a failure of the test An error is caused by the abnormal termination during the operation of the test whereas a failure of a test indicates that the test results were not within the specified limits If the pressure test fails e Ensure all fittings between the pump and the blank nut are tight Repeat the pressure test Often it is only a damaged blank nut itself poorly shaped from overtightening that causes the test to fail Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened If the test fails again insert the blank nut at the outlet of the previous module in the stack e g autosampler port
157. to the Agilent 1260 Infinity Binary LC System User Guide Degasser Pump 400 mm x 0 17 mm HiP AL 012 ice seat ca 320 mm x 0 12 mm Cooler for ALS New LowDelayVolume Heat Exchanger New LDV post column cooler TCC 0 12 mm ID cap 2 ul cell DAD Figure 7 Agilent 1260 Infinity Binary LC in low delay volume configuration for 2 1 mm and 3 0 mm id columns 1260 Infinity Binary Pump User Manual 35 3 Installing the Pump Optimizing the Stack Configuratior 36 1260 Infinity Binary Pump User Manual Installing the Pump 3 Optimizing the Stack Configuration Agilent 1260 Infinity Binary LC in Low Delay Volume Configuration In this configuration the LC is optimized for speed with the 2 1 mm columns For a more detailed help on configuring your instrument refer to the Agilent 1260 Infinity Binary LC System User Guide Degasser Pump 400 mm x 0 17 mm HiP AL 012 ice seat ca 320 mm x 0 12 mm Cooler for ALS New LowDelayVolume Heat Exchanger New LDV post column cooler TCC 0 12 mm ID cap 2 ul cell DAD Figure 9 Agilent 1260 Infinity Binary LC in low delay volume configuration for 2 1 mm and 3 0 mm id columns 1260 Infinity Binary Pump User Manual 37 3 Installing the Pump Agilent 1260 Infinity Binary LC in Low Delay Volume Configuration with Post Column Cooler This configuration is usually used for short 2 1 mm and 3 0 mm columns optimized for high flow rates For a more detailed help on co
158. tures It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 238 1260 Infinity Binary Pump User Manual This appendix provides general safety and environmental information 1260 Infinity Binary Pump User Manual 14 Appendix General Safety Information 240 The Waste Electrical and Electronic Equipment Directive 243 Lithium Batteries Information 244 Radio Interference 245 Sound Emission 246 Agilent Technologies on the Internet 247 ee Agilent Technologies 239 14 Appendix General Safety Information Safety Symbols Table 26
159. use accessory units tested and approved according to the safety standards appropriate for the type of external equipment CAUTION Agilent 1260 Infinity pump heads have been discontinued for Agilent 1260 Infinity Binary Pumps A number of 1260 Infinity Binary Pumps have been shipped with pump heads labeled as 1260 Infinity pump heads and passive inlet valves While these parts are as good from a performance perspective they are no longer used for 1260 Infinity Binary Pumps Parts listed in this manual are not compatible to 1260 Infinity pump heads and may get damaged gt Please contact your Agilent service representative 150 1260 Infinity Binary Pump User Manual Maintenance 10 Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 1260 Infinity Binary Pump User Manual 151 10 Maintenance Overview of Maintenance and Simple Repairs Overview of Maintenance and Simple Repairs Figure 23 on page 152 shows the main user accessible assemblies of the binary pump The pump heads and its parts require normal maintenance for example seal exchange and can be accessed from the fron
160. used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUTDOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal 226 1260 Infinity Binary Pump User Manual Hardware Information 13 Table 16 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module perf
161. vailable for the binary pump Agilent Technologies 125 8 Test Functions and Calibration System Pressure Test CAUTION This Lab Advisor test uses different names depending on the firmware revision used FW revision 2 A 06 50 System Pressure Test FW revision lt A 05 60 Pressure Test Description The system pressure test is a quick built in test designed to demonstrate the leak tightness of the system The test involves monitoring the flow profile while the pump delivers against a blank nut The result is presented as the leak rate of the module and provides information about the leak tightness of the system between the outlet valves of the pump and the blank nut The blank nut can be positioned anywhere between the purge valve of the pump and the detector inlet to pressure test the desired part of the system Blank nut placed at the outlet of flow cell The applied pressure may cause permanent leaks or bursting of the flow cell gt Never include the flow cell in the pressure test 126 Step 1 The test begins with the initialization of both pump heads After initialization the pump is starting the compression phase and the required flow rate is constantly monitored and adjusted The pump continues to pump until a system pressure of around 600 bar is reached Step 2 When the system pressure reaches 600 bar the pump continues to pump at a flow rate that keeps the pressure constant The flow that is neede
162. vent Selection Valve 48 Flow Connections without Solvent Selection Valve 51 Priming the System 54 Initial Priming 54 Regular Priming 56 Changing Solvents 57 This chapter gives information about the preferred stack setup for your system and the installation of your binary pump Apg Agilent Technologies 29 3 Installing the Pump Unpacking the Binary Pump Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 30 1260 Infinity Binary Pump User Manual Delivery Checklist Installing the Pump 3 Compare the delivery checklist with the contents of the shipping boxes to ensure completeness of the shipment The contents lists is shown below For parts identification check the illustrated parts breakdown in Parts and Materials for Maintenance on page 183 Please report missing or damaged parts to your local Agilent Technologies sales and service office
163. will turn off your pump automatically when running out of solvent A smarter way however is to use the bottle fillings function see Bottle Filling on page 67 Although channel A can be set to 0 it cannot be turned off This channel should be used for the aqueous phase water The percentage of channel B is automatically complemented by channel A to give 100 Select the solvent you are using in the respective solvent channel from the drop down list In case your solvent is not listed perform a solvent compressibility calibration see Running the Solvent Compressibility Calibration on page 134 For details on solvent compressibility see Binary Pump Solvent Compressibility Calibration on page 133 Free text field for a description of the solvent This description will show up in method printouts etc Use the timetable to build solvent gradients flow gradients or combinations of both Gradients are always linear Use multiple timetable entries to mimic exponential or parabolic gradients memory Display There are three ways to display the timetable in tabular form as flow pressure graph as solvent percentage plot Values can only be changed in tabular view 64 1260 Infinity Binary Pump User Manual Using the Pump 4 Pump Control The pump can be switched between following states On Off or to Standby In Standby the pump motor is still controlled When the pump is switched on from standby it d
164. y Pump User Manual 189 11 Parts and Materials for Maintenance Pump Head Assembly with Seal Wash Option 190 Item gt wo N 10 11 12 13 14 15 16a 16b 17 18 p n G1312 60045 5042 8507 5065 9978 5063 6586 G1311 60002 01018 60027 0905 1175 5062 2484 5042 8952 G1312 87300 5063 6589 0515 0175 G1312 23200 G1312 60061 G1312 60067 5042 1303 G1311 25200 G1312 60025 G1312 60020 G1312 23201 0515 2118 Description Pump head assembly with seal wash Peristaltic pump cartridge silicone tubing Tubing 1 mm i d 3 mm o d silicone 5 m for seal wash option Sapphire piston Piston housing Support ring seal wash Wash seal PTFE Gasket seal wash pack of 6 Seal holder Absorber capillary Piston seal PTFE carbon filled black pack of 2 default Mounting screw for manual purge valve holder M4 20 mm long Holder for manual purge valve Purge valve 1260 Outlet valve 1220 1260 Lock screw Pump chamber housing Active inlet valve body without cartridge Cartridge for active inlet valve 600 bar Adapter Pump head screw M5 60 mm 1260 Infinity Binary Pump User Manual Parts and Materials for Maintenance 11 Pump Head Assembly with Seal Wash Option Figure 28 Pump head assembly with seal wash option 1260 Infinity Binary Pump User Manual 191 11 Parts and Materials for Maintenance Outlet Valve p n Description G1312 60067 Outlet valve 1220 1260 Figure 29 Outlet Valve 192 1260 In
165. y volume configuration and gt 20 bar in standard configuration across the frit at a flow rate of 5 mL min of water with purge valve opened Purge valve if internally leaking p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 1924 Wrench open 14 mm Pair of tweezers Toothpick p n Description 1 01018 22707 PTFE frits pack of 5 1 G1312 60061 Purge valve 1260 1 5067 4728 Seal cap OPTIONAL Switch off pump at the main power switch Remove the front cover Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages 1260 Infinity Binary Pump User Manual Maintenance 10 Exchanging the Purge Valve Frit or the Purge Valve 1 Using a 1 4 inch wrench disconnect the pump outlet capillary from the purge valve Disconnect the waste tube Beware of leaking solvents due to hydrostatic pressure Pump outlet capillary Za 2 oy Purge valve Waste tube 2 Using the 14 mm wrench unscrew the purge valve and remove it from the purge valve holder 3 Remove the seal cap from the purge valve 4 Using a pair of tweezers or a toothpick remove the frit Valve body PTFE frit with groove Seal cap w 1260 Infinity Binary Pump User Manual 155 10 Maintenance Exchanging the Purge Valve Frit or the Purge Valve 5 Place a new frit into the purge valve with the orientation of the frit as shown below slit in frit points to the front Reinstall the seal c

Agilent 1260 Infinity Binary Pump User Manual

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