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Azure cSeries User Manual

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1. cSeries User Manual Page 19 17 On the Modify Repair or Remove Installation screen select MODIFY then NEXT and then INSTALL nna 18 On the next screen select FINISH modify Allows users to change the way features are installed 19 When the software launches automatically there will on be an error message if the system is not on Quit the See ag mir ae software and make sure the hardware is turned on and o Pease eee re launch the software L lt Back Copyright 2013 Azure Biosystems Inc All rights reserved The Azure Biosystems logo and Azure are trademarks of the Company SYBR is a trademark of Invitrogen Corporation Windows is a registered trademark of Microsoft Corporation in the United States and other countries All other trademarks service marks and trade names appearing in this brochure are the property of their respective owners Azure cSeries User Manual Page 20
2. Table of Specifications Image Resolution 5 4M pixels c200 8M pixels c300 c400 c500 600 Epi illuminations White LED Blue LED RGB LED c400 c600 660nm LD c500 c600 785nm LD c500 c600 Trans lllumination 302nm 365nm Lens 25mm F0 95 motorized c300 c400 c500 c600 8mm F 1 2 c200 Maximum Field of View 20cm x 15cm Image output 16 bit Tiff Jpeg Working environment Ambient temperature humidity 0 30 C 85 Power requirement 100 240 VAC 4A Dimensions W x D x H 38 cm x 30 cm x 45 cm Weight 22 kg 49 Ibs 2 Installation and Setup 2 1 System Placement Warning Excessive Weight Hazard Please use two or more people to lift the system Failure to do so can result in system damage and personal injury As with all electrical instruments the Azure cSeries imaging system should be located away from water solvents or corrosive materials on a flat and stable surface with adequate clearance on all sides The system must remain stationary during operation Further the system should be placed away from interfering electrical signals and magnetic fields If possible a dedicated electrical outlet should be used to eliminate electrical interference from other instrumentation in your laboratory The Azure cSeries imaging system should be installed at no more than 3000 meters above sea level 2 2 Connecting to Power The power entry module is located in the lower right on the back panel of the system Connect th
3. When taking a single channel image if marker is selected after capturing a chemi image the system will take a white light image of the blot The resulting image displayed in the gallery tab will show the chemi blot in the green channel and the white light image in the red channel This will allow you to overlay your signal over the image of your blot Note Maker feature works optimally when taken on the bottom UV transilluminator shelf position For all blots use the blot tray which is stored in the door The blot tray provides a dark background to improve the contrast on the blot image Azure cSeries User Manual Page 10 Positions for blot tray Chemi Shelf for chemi blots only UV Transilluminator for all blots 3 34 Imaging Workflow for Chemiluminescent Applications 1 Remove the Blot Tray from the cSeries door 2 After you have applied the substrate to your blot place your blot in the very center of the tray 3 Place the blot tray in one of two positions on the UV transilluminator or on the chemi shelf in the middle of the imaging system e Use the position on the UV transilluminator for larger blots e Use the position on the chemi shelf for small blots Using this top shelf increases the resolution of the blot 4 Close the door to the system 5 Select an exposure time e Recommendation Set the exposure time to 30 Seconds by hitting 3 then 0 and then S Keep the Sensitivity setting on the default s
4. If you select ROI a crop window will appear over you image By selecting the green edges of the box you can adjust the size of the box If you wish to crop you image select CROP on the lower right of the image Select CANCEL if you do not wish to crop the image ZOOM IN ZOOM OUT Use these features to increase or decrease the zoom factor on your image Azure cSeries User Manual Page 8 3 2 Gel Applications For applications that include EtBr SYBR Green Coomassie Blue or similar select the Gel tab You will see the following user interface AUTOEXPOSE Selecting autoexposure as the exposure time will allow the software to capture the image for you using the full dynamic range of the camera CAPTURE when you are ready to capture your image the capture button tells the system to start acquiring an image 3 24 Imaging Workflow 1 Select a light source You can select from UV 302 UV 365 EPI Blue or Visible The correct emission filter will be selected automatically e UV 302 For dyes that are UV excitable UV 302 will be the brightest and best light source e UV 365 For cutting bands out of a gel UV 365 reduces the nicks that might be introduced into the DNA by UV 302 EPI Blue Select this light source for gels that are stained with dyes that excite in the blue range Visible For dyes that are visible without additional excitation use the Visible light source When selected th
5. from coming in through the sides or around the lenses e Always cover all skin that may be exposed to UV light especially the face neck hands and arms Always make sure that any UV protection devices such as the safety switch on the light cabinet apparatus are working properly If not discontinue use until the device s are properly repaired e Please use only UV lamps in the transilluminator Electrical Safety Precautions Be sure to take proper precautions when handling any electrical equipment NEVER work on any live circuit fixture receptacle or switch Safety rules you should follow whenever working with any electrical appliance include e Always shut off power at the main disconnect before changing a fuse e Always shut off power to the circuit before repairing or replacing a switch receptacle or fixture e Always tape over the main switch empty fuse socket or circuit breaker you are working on e Always check that the circuit is dead before beginning work on it Using a circuit tester or voltmeter can help you determine this e Always unplug any appliance before repairing it Protective earth terminal The earth terminal intended for connection to an external protective conductor for protection against electric shock in case of a fault is located on the inside of the back panel Azure cSeries User Manual Page 1 Hot surface warning Under normal conditions the temperature of glass surface of UV transi
6. to a printer on the network CONTRAST Manually adjust the black white and gamma settings for the displayed image Recommended for chemiluminescent blots Note Adjusting these settings will not affect the raw data in your image AUTO Auto contrast will contrast your image so as to see the most features in the image INVERT Invert will display your image with dark and light pixels reversed SATURATION Selecting Saturation will show you images with intensities beyond the dynamic range of the camera SINGLE MULTICHANNEL VIEWING When a multicolor image is displayed in the gallery you will have the option of viewing single channels or all three channels at once By default the image is displayed as a multichannel image At this point contrasting the image will contrast all channels To view just one channel select the button that corresponds to the channel you wish to view To go back to the multichannel image select the button with the three overlapping colored circles ROTATE 90 DEGREES FLIP HORIZONTAL FLIP VERTICAL By select one or more of these icons you will shift the alignment of your image IMAGE INFO Image info displays information on how your image was acquired If the image was generated by an Azure cSeries system it will remember the selects that allowed you to acquire that image and display values such as image time filter settings and date the image was acquired ROI
7. ECTION WHEN EXPOSED TO UV LIGHT The use of protective eyeglasses mask and or gloves is strongly recommended After selecting the Band Excision button the user will be prompted to pull out the override safety interlocked switched pictured here Once the user pulls out the switch and selects OK from the screen prompt the light will remain on for 5 minutes or until the band excision switch is pressed again Capturing an image or selecting another light source will also turn the light off 3 3 Chemiluminescent Applications c300 systems and above MEES SENSITIVITY Changing the sensitivity settings changes both the sensitivity OW and the resolution of the camera and image At the Lowest sensitivity setting you will be taking a full resolution image with the camera And the Highest sensitivity setting the resulting image will be 0 3 MP image The resolution is displayed next to the sensitivity settings CUMULATIVE Selecting Cumulative will tell the software to take 5 images at the time interval you selected for exposure times For example if you set an exposure time of 1 minute it will display 5 images every 1 minute The first image displayed will be a 1 minute exposure The second image displayed at the 2 minute interval will be a sum of the first two images The finally fifth image will be the sum of 5 1 minute images You can view the images in the gallery tab while the next image is being acquired MARKER
8. QZUIC piosystems TM Azure cSeries User Manual for the c200 c300 c400 c500 and c600 EE E Safety and Regulatory Compliance Important Safety Information Please read these instructions before operating the Azure cSeries imaging system UV Safety Precautions Because U V radiation can cause serious damage CAUTI O N to unprotected eys and skin we recommend to wear U V protection glasses or face shield The Azure cSeries system comes with a built in Ultra Violet UV 200 400nm Transilluminator inside the system Exposure to UV radiation can cause permanent damage to the eyes and skin The system enclosure confines the radiation within the system and shields the user from exposure The system is also equipped with a two way safety interlock switch which automatically cuts off the power to the transilluminator when the door is open during normal use The Azure cSeries imaging systems belongs to Class A equipment and fulfills the limit values of table 3 but not table 4 of EN 61326 1997 A1 A2 A3 It may become necessary to defeat the safety lock or operate the transilluminator outside the system for service In this case be sure to use the following safety precautions e Always wear UV protected eyewear that is specified by the manufacturer as providing protection at the wavelength s used making sure that the eyewear protects any areas where radiation may come through UV sunglasses may not prevent UV radiation
9. URITY WARNING N O OO A OW Publisher of the software Select RUN 8 On the setup screen select NEXT 9 On the next Setup screen select the cSeries model that you have Azure cSeries User Manual Programs Eg Uninstall a program From the list of programs to uninstall select CSERIES CAPTURE SOFTWARE Once it is uninstalled you can begin installing the new software A User Account Control Window will pop up and ask you to verify that you know the Setup Welcome to the cSeries Capture Software Setup Wizard This will install cSeries Capture Software version 1 1 0 0117 your computer It is recommended that you close all other applications before continuing Click Next to continue or Cancel to exit Setup Select your cSeries model Please specify which cSeries model you have then click Next c600 features NIR RGB Chemi Gel 500 features NIR Chemi Gel C c400 features RGB Chemi Gel 300 features Chemi Gel c200 features Gel only Page 18 10 Accept the License Agreement eae sa important information before continuing Please read the following License Agreement You must accept the terms of this agreement before continuing with the installation cSeries Capture Software License Agreement California corporation Our contact details are listed on our website at http www azurebiosystems com about cont act us php The term yo
10. ange of applications Contact us at info azurebiosystems com to learn more The Azure cSeries system includes the following key components Azure cSeries User Manual The Cabinet The cabinet is a light tight image station Camera High resolution camera preinstalled in the system A motorized lens is included on the c300 systems and above UV Transilluminator The UV illuminator provides 302nm and 365nm trans illumination It is mounted on a pull out tray EPI Blue and White LEDs The cabinet also contains low intensity Epi white and blue light panels on each side of the cabinet interior walls EPI RGB LEDs High intensity LED illuminators c400 and c600 EPI 660nm amp 785nm Laser Diodes c500 and c600 Filters For each light source appropriate filters are also included in the motorized filter wheel Tablet PC The tablet PC is a self contained touch screen PC for system control and image acquisition Orange Tray For Visible dyes the orange tray converts UV light into Visible light Blot Tray Stored in the cSeries door the Blot Tray is required for imaging all blots For fluorescent blots it is placed on the UV transilluminator For Chemi blots it can be placed on the UV transilluminator or on the chemi shelf in the cabinet USB Memory Stick The Azure Biosystems memory stick includes the analysis software and any other files pertinent to your system AzureSpot Analysis software Page 5
11. e power cord to a secure power outlet It is important to connect the system to a well grounded power source Azure Biosystems recommends that you employ a surge protecting power strip to protect against potential electrical component damage from power surges 2 3 Power On Off the System Power on the system by 1 Using the power switch on the back of the instrument 2 Pressing the power button on the top of the tablet Azure cSeries User Manual Page 6 To turn off the system shut down the tablet by 1 Swiping from the right to left on the right side of the screen a Select SETTINGS b Select POWER c Select SHUT DOWN 2 Turn off power to the system using the power switch on the back of the instrument Azure Biosystems recommends leaving the system on during working hours as the system takes about 20 minutes for the camera to cool after first power up and first launch of the software 2 4 Software Installation The Azure cSeries Acquisition Software is pre installed When the tablet is powered on from being in the off state the software should launch directly into the cSeries capture software application Otherwise to launch the software double click on the desktop icon The system will take a few seconds to initialize You will see the following screen during initialization Once the system is done initializing the software displays the gallery tab and is ready to use 2 5 Attach Other USB Input Device
12. e software will prompt you to use the Orange Tray which converts the UV light into bright visible light to provide contrast to your image 2 Place the sample on the UV transilluminator or on the Orange Tray for Visible samples 3 Select an imaging time a Manual Imaging times are entered using our keypad For gels we recommend setting exposure time to less than 1 minute e For 20 seconds hit 2 then 0 then S To select 1 5 seconds type 1 then S and then 500 and MS e To erase what you done hit the C button for clear b Or you can select AUTOEXPOSE which will calculate the best imaging time for you image Selecting autoexpose will grey out the keypad enabling you to simply press capture c Maximum exposure time is 30 seconds 4 Hit CAPTURE The software will direct the camera to collect the image and the final image will be displayed in the Gallery tab Azure cSeries User Manual Page 9 3 28 Band Excision and Light Safety Switch The light safety interlock switch is located on the front edge of the system and is visible when the door is open Normally if UV light is being used the cabinet automatically shuts off the UV light source when the door is opened The BAND EXCISION button was created to override the safety switch for situations when a user needs to cut bands out of their UV excitable gel Care must be taken to avoid eye and skin exposure to the UV radiation WARNING ALWAYS WEAR APPROPRIATE PROT
13. ed by anyone other than Azure Biosystems Damage caused by accident or misuse Damage caused by disaster Corrosion caused by improper solvents or samples Voltage Setting Information The Azure cSeries imaging system has a power supply that automatically chooses the correct voltage for your country or region CE Conformity The following Azure Imaging Systems models c200 c300 c400 c500 c600 are in conformity with the provisions of the following EC Directives including all amendments and national legislation implementing these directives Low Voltage Directive 2006 95 EC EMC Directive 2004 108 EC And that the following harmonized standards have been applied EN61010 1 2001 EN61326 1 1997 A1 1998 A2 2001 A3 2003 Class A EN61000 4 2 EN61000 4 3 EN61000 4 4 EN61000 4 5 EN61000 4 6 EN61000 4 1 1 Protection category IP20 according IEC 60529 Contact Azure Biosystems Inc 6773 Sierra Court Unit B Dublin CA 94568 USA info azurebiosystems com 925 307 7127 Fax 925 307 7287 Azure cSeries User Manual Page 3 Table of Contents 1 Introduction Table of Specifications 2 Installation and Setup 2 1 System Placement 2 2 Connecting to Power 2 3 Power On Off the System 2 4 Software Installation 2 5 Attach Other USB Input Devices to the System 3 cseries Capture Software Overview 3 1 Gallery 3 2 Gel Applications 3 2A Imaging Workflow 3 2B Band Excision and Light Safety Switch 3 3 Chemilumine
14. elect the resolution The higher the resolution the longer we recommend setting your exposure time Azure cSeries User Manual Page 12 5 Select CAPTURE The channels will be captured sequentially 6 The resulting image will be displayed in the gallery tab 3 6 Custom Applications The custom tab allows the users full flexibility when acquiring their image LOAD PROTOCOL Load Previously saved protocols for quick image capture SAVE PROTOCOL If you have a custom setting you plan to use multiple times save it for quick recall in the future Imaging workflow 1 Select the sensitivity resolution for your setting To use the full camera resolution select LOWEST 2 Select the light sources you wish to use a If you wish to take a single channel image select a light source next to GRAYSCALE b If you wish to take a multichannel image make sure the imaging time next to Grayscale is set to 0 Then select the light sources you would like to image next to the color channel you would like them assigned to Set the exposure time for each channel 3 Select the aperture setting the smaller the number the wider the aperture For chemiluminescence we recommend 0 95 for fastest light gathering For all other applications 1 4 is recommend for improved depth of field and focus 4 Select the focus setting For chemi applications on the Blot Tray on the UV transilluminator we suggest 2600 For chemi applications on
15. etting NORMAL Select CUMULATIVE After hitting CAPTURE a series of images will be displayed and you can select the best one from the gallery tab e Maximum exposure time is 30 minutes 6 Select CAPTURE 7 The images will be displayed in the gallery tab 3 4 RGB Applications c400 and c600 Selecting RBG will give you the follow screen The RGB setting is for fluorescent Western blots labeled with Cy5 Cy3 Cy2 or dyes with similar excitation and emission spectrums For all RBG blots use the Blot Tray on the UV transilluminator position only The blot tray provides a dark background to improve the contrast on the blot image Placing this Tray on the UV transilluminator position provides even illumination for your samples Azure cSeries User Manual Page 11 For each wavelength you can set a unique exposure time Each channel will be captured in a unique color channel and will be displayed in a multicolor overlayed image You can take a 1 2 or 3 channel image 1 Place blot in the center of the Blot Tray 2 Close the door 3a Manual Exposure e Touch the exposure time box next to the channel that you want to image Type in an exposure time using the keypad See section 3 2A on how to use the keypad e To take a multi channel image such as a Cy5 Cy3 image enter an exposure time for both the Cy5 and Cys channel e For all channels you do not want to image leave the exposure time at 0 e Maximum exposure time
16. her or both laser modules Users or their service technicians should make no attempt to determine the cause of faulty laser operation and should promptly contact Azure Biosystems at support biosystems com or their nearest Azure Biosystems authorized service location Azure cSeries User Manual Page 2 Caution Azure Biosystems c500 and c600 systems contain a defeatable safety interlock system It is not recommend or advised by Azure Biosystems under any circumstances for users to defeat the interlock system and perform laser imaging or imaging with any c500 or c600 light source with the access door open The access door must be fully closed For Research Use Only This instrument is suitable for research use only It must be used therefore only by personnel who know the health risks associated with the reagents that are normally used with this instrument Warranty The Azure cSeries products are warranted against defects in materials and workmanship for one year unless otherwise outlined on your sales order or www azurebiosystems com warranty If any defect occurs in the instrument during this warranty period Azure Biosystems Inc will repair or replace the defective parts at its discretion without charge The following defects however are specifically excluded Defects caused by improper operation Repair or modification done by anyone other than Azure Biosystems or the company s authorized agent Use of spare parts suppli
17. ight and left laser modules located in the c500 and c600 imaging chamber are installed in a highly diffused protective metal enclosure preventing exposure to full laser radiation power In addition the entire laser system is fully enclosed within the system enclosure and there are no viewing ports windows or openings to facilitate viewing of or exposure to laser radiation fields from direct impact reflection or leakage Maintenance Azure Biosystems c500 and c600 laser systems do not require regular periodic or preventive maintenance in the form of adjustments calibrations cleaning or other standard maintenance procedures to maintain optimal performance thereby removing the need for users or their service technicians to initiate any actions where exposure to laser radiation would occur Serviceability Replacement of faulty laser modules is a manufacturer only repair action and not a customer service action Laser repair or replacement may be performed in the field by Azure Biosystems authorized service technicians or by return of the entire system to Azure Biosystems or its authorized service location s for laser repair or replacement Lasers are deemed to be faulty or defective if users discover images that show evidence of output signal level loss in either the left or right laser module a significant difference between the output signal levels between the left and right lasers modules or complete loss of output signal level in eit
18. is 5 minutes per channel 3s Auto exposure autoexpose will calculate the best imaging time for you image After selecting AUTOEXPOSE simply press GAPTURE You can choose auto exposure for all one or two channels 4 Select the resolution The higher the resolution the longer we recommend setting your exposure time 5 Select CAPTURE The channels will be captured sequentially 6 The resulting image will be displayed in the gallery tab 3 5 NIR Applications c500 and c600 For all NIR blots use the Blot Tray on the UV transilluminator Qala position only The blot tray provides a dark background to improve the contrast on the blot image Placing this Tray on the UV transilluminator position provides even illumination for your samples 1 Place blot in the center of the Blot Tray on the UV transilluminator position 2 Close the door 3a Manual Exposure e Touch the exposure time box next to the channel that you want to image Type in an exposure time using the keypad See section 3 2A on how to use the keypad e To take a multi channel image enter an exposure time for both the 700 and 800 channel e For all channels you do not want to image leave the exposure time at 0 e Maximum exposure time is 5 minutes per channel 3s Auto exposure autoexpose will calculate the best imaging time for you image After selecting AUTOEXPOSE simply press CAPTURE You can choose auto exposure for all one or two channels 4 S
19. lder 3 Launch the software Appendix B Changing the UV Transilluminator There are no field serviceable parts on the Azure c system It is likely that the cold cathode fluorescent light bulbs will be burned out despite their exceptional long life as compared to the traditional fluorescence light bulbs used in other UV transilluminator Because the light bulbs are not made to be replaced by the end user Azure will provide the replacement for the complete UV transilluminator module when the light bulbs go bad Please use the following procedure the replace the UV transilluminator 1 2 3 Azure cSeries User Manual Disconnect the power cable Push both the left and right locking lever on the slide rails upwards Make sure the system is powered off Unplug the power cord from the from power source Open the system door and pull out the transilluminator Pull the transilluminator out of the rail Reverse step 3 6 to install a new transilluminator Appendix C Installing a New Version of the cSeries Capture Software c300 c600 Please note you need to be logged in an Admin account to install new software on your computer If you are using the Azure provided tablet the admin password is admin 1 Swipe from the right of the screen and click the SETTINGS tab 2 Select CONTROL PANEL then UNINSTALL A PROGRAM Select the SETUP file in the new software folder Select RUN from the OPEN FILE SEC
20. lluminator is below 50 C and safe to touch However if the system malfunctions it is possible that the glass surface temperature exceeds 80 C Please exercise caution when touch the glass surface with hand when this occurs Laser Safety General Information Azure Biosystems imaging system models c600 and c500 both include a laser illumination system that provides 660nm and 785nm Narrow band excitation for NIR applications The imaging systems are certified to comply with CE UL and CSA safety standards and consists of two fully enclosed electronic modules located on the right and left interior sides of the imaging chamber This laser system is by its appropriate classification and definition a non removable laser system as it is not operable when the laser modules themselves are removed from within the system Safety Features Azure Biosystems imaging systems are designed to prevent direct and collateral human exposure to laser radiation by means of a safety interlock switch located on the right front side of the imaging chamber The safety interlock reacts to door open and door closed states and defeats all power to internal light sources when the door is in the open position Lasers and other internal system light sources will not power on unless the door is fully closed If the access door is opened during imaging all light sources will immediately power off to prevent human exposure to internal illumination sources R
21. ow you to access this PDF For further help please email us at info azurebiosystems com 6 Quit Selecting QUIT will return you to the Windows 8 desktop 7 Tablet and Windows tools Tablet Specifications Processor Speed 1 50 GHz Screen Resolution 1366 x 768 Processor Core Dual core 2 Core Wi Fi Yes Cache 1 MB Wi Fi Standard IEEE 802 11a b g n Standard Memory 2 GB Bluetooth Yes Flash Memory 32 GB Operating System Windows Capacity Platform Screen Size 10 1 Operating System Genuine Windows 8 Screen Mode HD Operating System 32 bit Aspect Ratio 16 9 Architecture Azure cSeries User Manual Page 14 To help those who aren t familiar with the Windows 8 here are a few key tips 7 1 How to Power Off Restart the Tablet To turn off the system shut down the tablet by a Swiping from the right to left on the right side of the screen to bring up the right side toolbar b Select SETTINGS c Select POWER d Select SHUT DOWN or RESTART 7 2 How to Access the Keypad The keypad can be accessed on the bottom screen menu bar Azure cSeries User Manual Page 15 Appendix A Running the System from an External Computer If you would like to run your system from an external PC or Laptop for service or personal reasons follow these instructions You will need e A3mm hex key e A standard USB 2 0 cable with a USB type A termination at one end and a type B termination at the other e A Windows 8 laptop o
22. r desktop Before performing any maintenance on the system make sure the system power switch is in the off position the unit is unplugged from the wall and that the tablet is off Hardware 1 Use a 3mm hex key to loosen the 6 screws on the back of the unit 2 Gently and slowly pull the back panel of the unit off e Two cables prevent you from completely removing the back panel of the unit Caution The power connectors for the fans are attached to the back of the unit Do not pull the back panel off the unit until you have unplugged them 3 Unplug the fan power connectors 4 Unplug the USB connector from the back of the unit e Disconnect the tablet from the USB hub Empty USB A port once tablet is unplugged Azure cSeries User Manual Page 16 5 Plug in the USB 2 0 cable into the back of the unit 6 Power on the imaging system Vi on and then into your laptop or desktop N o o o lt E Software Ti On the USB that is included with the system find the Azure cSeries Capture software Plug the USB onto your Win 8 computer and run the installer for the cSeries capture software Follow Appendix C Installing the Azure cSeries Capture software Once the installer is done copy the cSeries folder found on the USB in the Calibration folder to C ProgramData Azure Biosystems and replace the existing cSeries folder If promoted copy over all existing files in the cSeries fo
23. s to the System You may attach regulatory approved Windows OS supported USB keyboard USB mouse or other USB input devices to any of the three external USB port on the system 3 cSeries Capture Software Overview Azure cSeries captures high quality images with a very simple and intuitive user interface The icon based software allows you to set up the instrument for different applications by simply touching one button There is no need to focus on your sample you simply need to place the sample in the system and select an exposure time 3 1 Gallery When the software opens it launches into the Gallery window designed for opening closing saving and printing your images It also allows you perform basic image editing on your opened image OPEN Use this icon to open an image that is stored locally on a USB or on the network for systems that are networked Azure cSeries User Manual Page 7 SAVE Allows you to save you image to a USB to the tablet or to a network drive Images can be saved as a Tiff or Jpeg file File names are automatically generated with a date and time stamp but you can override the automatically generated name by touching the displayed textbox A keypad will appear for you to name your file Note Azure does not recommend saving images to the tablet computer in order to maintain a fast processing speed for the application PRINT Allows you to print to a printer attached to the system or
24. scent Applications c300 systems and above 3 3A Imaging Workflow for Chemiluminescent Applications 3 4 RGB Applications c400 and c600 3 5 NIR Applications c500 and c600 3 6 Custom Applications 3 7 Save Acquired Image 4 Settings 5 Help 6 Quit 7 Tablet and Windows Tools Tablet Specifications 7 1 How to Power Off Restart the Tablet 2 How to Access the Keypad Appendix A Running the System from an External Computer Hardware Software Appendix B Changing the UV Transilluminator Appendix C Installing a New Version of the cSeries Capture Software Azure cSeries User Manual O Oo NN DD D O ONN 10 11 11 12 13 13 14 14 14 14 14 15 15 16 16 17 17 18 Page 4 L Introduction The Azure cSeries imaging systems offer high performance imaging for a wide variety of applications The cSeries family of instruments includes the follow products c200 c300 c400 c500 and c600 Select which one fits your applications now and learn more about upgrading later when your needs change c600 c500 c400 c300 c200 _ i aa CJ To Z ET m c Oz eh jea CHEMI 9 E rs BLUE WHITE CHEMI LIGHT LIGHT UV ae F rcB cHemi BOVE WHITE uv BLUE WHITE CHEMI LIGHT LIGHT UV BLUE WHITE uy LIGHT LIGHT Each Azure cSeries imaging workstation can be upgraded in the field to a higher model with a wider r
25. the Blot Tray on the chemi shelf we suggest 8400 For NIR applications we suggest 3700 4200 For RGB applications we suggest 2400 3000 o 29 5 For gel applications we suggest 2400 5 Select the number of frames 6 Save your protocol if you think you will use it in the future 7 Hit CAPTURE The resulting imaging will be displayed in the gallery tab 3 Save Acquired Image Users are discouraged to store image files on the tablet since its disk space and memory resources are limited Please use a USB storage device or if the system is networked save to a network location There are three USB port one in the front and two on the right side panel Azure cSeries User Manual Page 13 To save an image select SAVE from the gallery tab A file name is auto populated but this can be changed by touching the entry area and a keyboard will pop up Images can be saved as Tiff or Jpeg files Tiff files are recommended if the image is later going to be analyzed 4 Settings The settings tabs allows you access to features that are needed to calibrate the system RECREATE DARKMASTER Darkmasters are created at the factory but in the event REATI that the camera is replaced creating darkmasters in the field can be done FOCUS CALIBRATION Allows the user to reset the focus setting in case of a shift in focus CREATE FLATS Allows users to recreate flat calibration files 5 Helo Selecting the help tab will all
26. u refers to the 1 do not accept the agreement Cre 11 Select the destination you would like to install the software We recommend the default Select Destination Location Where should cSeries Capture Software be installed 12 If it asks you to replace the existing cSeries folder select YES then INSTALL a Setup will install cSeries Capture Software into the following folder To continue click Next If you would like to select a different folder click Browse c Program Files x86 Azure Biosystems cSeries Browse At least 30 7 MB of free disk space is required lt Back Next gt 13 On the CP210x USB to UART Bridge Driver Installer CP210x USB to UART Bridge Driver Installer Wel the CP210x USB screen j select N EXT s u UART Bridge Driver Tnetaller This wizard will help you install the drivers for your CP210x USB 14 On the next screen accept the agreement an aie 15 Select FINISH on the next screen in this installation process To continue click Next lt Back 16 The next screen will be the 32 bit software setup screen Select NEXT Welcome to the Apogee 32bit Software Setup Wizard The Setup Wizard will allow you to change the way Apogee 32bit Software features are installed on your computer or even to remove Apogee 32bit Software from your computer Click Next to continue or Cancel to exit the Setup Wizard Azure

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