Cary Eclipse Fluorescence Spectrophotometer
Contents
1. Cary Eclipse Fluorescence Spectrophotometer User s Guide eh Agilent Technologies Notices Agilent Technologies Inc 1999 2000 and 2011 2013 2015 No part of this manual may be reproduced in any form or by any means including electronic storage and retrieval or translation into a foreign language without prior agreement and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 8510175800 Edition Fifth edition October 2015 Printed in Malaysia Agilent Technologies Australia M Pty Ltd 679 Springvale Road Mulgrave Victoria 3199 Australia Agilent products must only be used in the manner described in the Agilent product user guides Any other use may result in damage to the product or personal injury Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations Agilent shall not be liable for errors or damages on products or performance due to using non Agilent genuine parts Warranty The material contained in this document is provided as is and is subject to being changed without notice in future editions Further to the maximum extent permitted by appl2. 3 Disconnect the Cary Eclipse from the mains power supply Remove the lamp access panel see Figure 1 Facing the front of the Eclipse turn the instrument onto its right side to get access to the base On the base of the instrument undo and remove the four thumb screws of the lamp module cover see Figure 3 Figure 3 Base of the Eclipse showing the lamp module cover thumb screws 1 5 Loosen the three bolts marked with a black dot labeled 1 in Figure 4 until the lamp module is free to move through the front opening Figure 4 Lamp module showing 1 Bolts and 2 Plug Cary Eclipse Fluorescence Spectrophotometer User s Guide 33 Maintenance and Spare Parts 34 Move the lamp module towards the front of the instrument to disengage two guide pins at the rear Partially withdraw the module through the opening in the base Squeeze the clip on the plug labeled 2 in Figure 4 and remove it from its socket Completely withdraw the lamp module Eye and Electrical Shock Hazards When operating the lamp module emits high intensity light which can damage eyes To avoid eye damage never operate the lamp module outside the instrument The lamp module contains components operating at high voltages To avoid electric shock NEVER disassemble the lamp module To install the lamp module 1 Plug the connector of the new lamp module into the socket in the instrument Fit the lamp module in the base
3. 3 Cell lifter and height adjuster 4 Cell holder thumbscrew 5 Pitch adjustment screw 4 Place the single cell holder on the cell holder base so that the cell lifter 8 is at the rear of the sample compartment 5 Secure the cell holder by tightening the spring loaded thumbscrew 4 Cuvette height adjustment e Turning the cell lifter 3 in a clockwise direction will raise the cuvette e Turning the cell lifter 8 in an counter clockwise direction will lower the cuvette platform To lower the cuvette you will have to push the cuvette down because of the tension created by the single cell holder Cary Eclipse Fluorescence Spectrophotometer User s Guide 23 Installation 24 Cuvette pitch adjustment 1 2 Turning the pitch adjustment screw 5 in a clockwise direction will raise the front of the cell holder Turning the pitch adjustment screw 5 in an anti clockwise direction will lower the front of the cell holder The pitch adjustment screw requires a 2 5 mm hex key Aligning To align the single cell holder containing a microcell 1 Start the Align application by clicking the Start button in the Windows taskbar and choosing All Programs gt Agilent gt Cary Eclipse gt Align Click the Cary tab Place a fluorescent sample for example anthracene in a microcell and adjust the excitation and emission wavelengths to get a signal Click Apply The wavelengths should change and the green power in
4. This radiation can cause serious damage to eyes Never look directly at the UV source lamp Always wear safety glasses manufactured to an approved standard and which are certified or otherwise warranted to protect the eyes from UV radiation when the lamp is operating and the sample compartment is open Electrical hazards The Cary Eclipse system and some accessories contain electrical circuits devices and components operating at dangerous voltages Contact with these circuits devices and components can cause death serious injury or painful electrical shock Panels or covers which are retained by screws on the spectrophotometer and accessories and carry a warning may be opened ONLY by Agilent trained Agilent qualified or Agilent approved customer service representatives Consult the manuals or product labels supplied with your computer monitor and printer to determine which parts are operator accessible Cary Eclipse Fluorescence Spectrophotometer User s Guide Safety Practices and Hazards Good grounding is essential to avoid a potentially serious electric shock hazard Ensure that there is an integral ground connection between the metal base of the spectrophotometer and accessories and the three pin earth grounded receptacle Consult the manuals or product labels supplied with your computer monitor and printer for their grounding requirements The safety classification is given as Equipment Class I Application of the wrong s
5. dimensions 19 Power 19 Connections 19 Cary Eclipse Fluorescence Spectrophotometer User s Guide 3 Contents Fuses Nitrogen supply Computer placement 3 Installation Single cell holder Aligning Other sample holders Purging Nitrogen Pressure regulator and gauge Supply tubing Gas manifold Flowmeter Installing the software Installing the USB GPIB HS Converter Registering the Cary Eclipse software Uninstalling the software Software Status Bulletin 20 20 20 21 22 24 25 25 25 25 26 26 26 26 28 28 29 29 Cary Eclipse Fluorescence Spectrophotometer User s Guide Contents 4 Maintenance and Spare Parts 31 Cleaning 32 Lamp module 32 Replacing 32 Aligning 35 Fuses 38 Replacing 38 Checking 39 Spare Parts 39 5 Troubleshooting 41 Instrument offline 42 Connect button instead of Start 42 Instrument performance testing 43 Cary Eclipse Fluorescence Spectrophotometer User s Guide 5 Contents This page ts intentionally left blank 6 Cary Eclipse Fluorescence Spectrophotometer User s Guide Safety Practices and Hazards e e 1 Safety Practices and Hazards amp Lamp module 7 s g m Ultraviolet radiation 8 ig Electrical hazards 8 Panels covers and modules 9 Other precautions 10 Warning and information symbols 11 Color coding 13 CE compliance 13 Electromagnetic compatibility 14 Your Agilent Cary Eclipse instrument and accessories have been carefully designed s
6. of the Cary Eclipse ensuring that the two guide pins are correctly located and that the wiring is kept clear of the other parts of the instrument Tighten the three bolts through the front panel Replace the lamp access panel Replace the lamp module cover and tighten the four thumbscrews Return the instrument to its upright position Replace the power cable at the rear Turn on the computer and the instrument After replacing the lamp module you must align the lamp module Cary Eclipse Fluorescence Spectrophotometer User s Guide Maintenance and Spare Parts Aligning For optimum performance of the instrument the lamp module must be aligned To align the lamp module The instrument power must be on for the alignment procedure 1 Start the Align application by clicking the Start gt Programs gt Agilent gt Cary Eclipse gt Align 2 Remove the lamp access panel see Figure 1 to access the lamp alignment screws 3 Inthe Align application click the Cary tab and set up these instrument parameters Ex Wavelength nm 540 Ex Slit nm 1 5 Ave time s 0 1 PMT voltage Medium Emission parameters are not important for this procedure Click the Graph tab View the reference signal by clicking the Reference Signal button on the toolbar 6 Click the Vertical Rescale button J to find the signal range 7 Click the Scale button F and enter an upper limit that is 20 higher than the current signal and a
7. when lifting or carrying the instrument Never attempt to lift the instrument alone Following the instructions in the site preparation guide you should have e Unpacked the fluorescence spectrophotometer and placed it on the workbench e Unpacked the computer and placed it on the workbench e Installed the operating system software on the computer Cary Eclipse Fluorescence Spectrophotometer User s Guide 21 Installation 22 This chapter describes how to install the e Sample holders used with the Cary Eclipse e Cary Eclipse software in case you ever need to reinstall it Instructions for installing replacing the lamp module are included in the Maintenance chapter Single cell holder A single cell holder is supplied as standard with the Cary Eclipse Follow these instructions to install and align it To install the single cell holder 1 Slide back the sample compartment lid 1 in Figure 1 lift up and remove the front panel 2 and check that the sample compartment is empty Figure 1 Cary Eclipse showing the sample compartment lid 1 and front panel 2 and Lamp Access Panel 3 2 Position the cell holder base on the locating pins Cary Eclipse Fluorescence Spectrophotometer User s Guide Installation 3 Lock the cell base by turning the lever 2 in Figure 2 to the right Figure 2 Cell holder base left and Single cell holder 1 Locating holes 2 Cell holder locking lever
8. A GB e C US K When attached to the rear of the product indicates that the product 12 Cary Eclipse Fluorescence Spectrophotometer User s Guide Safety Practices and Hazards Color coding The various indicator lights appearing on Agilent instruments and associated accessories are color coded to represent the status of the instrument or accessory e A steady green light indicates the instrument is in normal or standby mode e A flashing green light indicates the instrument is scanning reading e A steady orange light indicates the instrument is starting up e A flashing orange light indicates that there is a problem with the instrument and it may require servicing e lt A steady yellow light indicates that the instrument is initializing CE compliance Agilent Cary Eclipse instruments have been designed to comply with the requirements of the Electromagnetic Compatibility AMC Directive and the Low Voltage electrical safety Directive commonly referred to as the LVD of the European Union Agilent has confirmed that each product complies with the relevant directives by testing a prototype against the prescribed EN European Norm standards Proof that a product complies with the directives is indicated by e The CE marking appearing on the rear of the product e The documentation package that accompanies the product containing a copy of the Declaration of Conformity This declaration is the legal declaration by Agilen
9. ENTER and press SHIFT F8 Specifications The instrument is suitable for indoor use only and is classified Pollution degree 2 and Installation Category II EN 61010 1 Environmental Table 1 Suitable conditions during instrument transportation non operation and operation Condition Altitude m ft Temperature C F Non operating transport 0 2133 0 7000 5 45 41 113 Operating within performance 0 853 0 2800 10 35 50 95 specifications 853 2133 2800 7000 10 25 50 77 Relative humidity non condensing 20 80 8 80 For optimum analytical performance it is recommended that the ambient temperature of the work area be between 20 and 25 C 68 and 77 F and be held constant to within 2 C throughout the entire working day 18 Cary Eclipse Fluorescence Spectrophotometer User s Guide Introduction Weights and dimensions Weight Packed 54 kg 119 Ib Unpacked 31 kg 68 Ib Dimensions width x depth x height Packed 820 x 760 x 560 mm 82 x 30 x 22 in Unpacked 600 x 610 x 280 mm 24 x 24 x 11 in Power e Voltage 100 240 VAC e Frequency 50 60 Hz Hz e Consumption 180 VA approximately e Mains inlet coupler 3 2 A 120 250 VAC 50 60 Hz IEC type Connections Mains power cord Only use the country specific Agilent supplied power cord with your Cary Eclipse Front Accessory connection 25 pin D range connector low voltage DC AC and digital analog lines Po
10. Operators and other unauthorized personnel are permitted access ONLY to the lamp module and the sample compartment of the Cary Eclipse ALWAYS switch off the instrument and disconnect the mains cord before changing a lamp module Other precautions The lamp module operates at a high temperature and touching it may result in burns Before replacing a lamp module that has been operating switch off the Cary Eclipse and ensure that the lamp module has cooled or protect your fingers from burns Do not block the ventilation grilles on the spectrophotometer and accessories Consult the manuals supplied with your computer monitor and printer for their specific ventilation requirements Use of the Cary Eclipse system and accessories may involve materials solvents and solutions which are flammable corrosive toxic or otherwise hazardous Careless improper or unskilled use of such materials solvents and solutions can create explosion hazards fire hazards toxicity and other hazards which can result in death serious personal injury and damage to equipment and property ALWAYS ensure that laboratory safety practices governing the use handling and disposal of such materials are strictly observed These safety practices should include the wearing of appropriate safety clothing and safety glasses 10 Cary Eclipse Fluorescence Spectrophotometer User s Guide Safety Practices and Hazards Warning and information symbols The following sym
11. allation requirements Before receiving your instrument you will have been provided with a Cary Eclipse Site Preparation Guide which describes the environmental and operating requirements of the Agilent Cary Eclipse system You must prepare your laboratory according to these instructions before the Cary Eclipse can be installed You should keep the site preparation guide for future reference If you have misplaced your copy you can obtain a replacement from your local Agilent office or from the Agilent website www agilent com Documentation You have been provided with the following documentation to help you set up and operate your Cary Eclipse system e This user s guide with safety practices and hazards information instructions for installing and maintaining the components of the Cary Eclipse and software and troubleshooting information e Extensive Help provided with the Cary Eclipse software containing context sensitive Help step by step instructions for frequently performed analyses and instructions for using any accessories you ordered Cary Eclipse Fluorescence Spectrophotometer User s Guide 17 Introduction Conventions The following conventions have been used in procedures throughout the documentation e Menus menu items buttons and check boxes have been typed in bold for example click OK and From the Edit menu choose Copy e ALL CAPITALS indicate keyboard commands For example press
12. bols appear in conjunction with warnings on the spectrometer and associated documentation The hazard they depict is shown below each symbol gt Corrosive liquid Ejecting parts Electrical shock gt gt Extreme cold Eye hazard Fire hazard gt Heavy weight Heavy weight Hot surface danger to feet danger to hands gt Moving parts Noxious gas Sharp edge The following symbol may be used on warning labels attached to the instrument When you see this symbol refer to the relevant operation or service manual for the correct procedure referred to by that warning label Cary Eclipse Fluorescence Spectrophotometer User s Guide 11 Safety Practices and Hazards The following symbols also appear on the instrument or in the documentation l Mains power on 0 Mains power off ON Single phase alternating current nimmen Fuse G Indicates that a high voltage xenon flash lamp is present Xe When attached to the rear of the instrument indicates that the product complies with the requirements of one or more EU directives When attached to the rear of the product indicates that the product has been certified evaluated to CSA 61010 1 and UL 61010 1 Disconnect all power plugs complies with the South Korean Electromagnetic Compatibility EMC law applicable to Industrial and scientific use equipment When attached to the rear of the instrument indicates that the product complies with the requirements of ACM
13. dicator on the instrument should continue flashing to indicate that the instrument is active Place a small piece of white paper in the light path to the right of the cell use a wavelength of 540 nm If the beam appears as though it will strike the cell aperture move the paper to the left of the cell and check that the beam is passing through the cell If the beam does not appear as though it will pass through the cell adjust the height of the cell as described on Page 23 Using the 2 5 millimeter hexagonal ball driver adjust the pitch adjustment screw 5 in Figure 2 and note the intensity of the light striking the paper Continue to adjust the adjustment screw until the beam hitting the paper appears the most intense You may need to dim the room lights to see the light beam Cary Eclipse Fluorescence Spectrophotometer User s Guide Installation Other sample holders Purging Other sample holders are available for use with the Cary Eclipse Instructions for their use are included in the Help provided with the Cary Eclipse software The nitrogen purging system is not supplied by Agilent but the following items are available from appropriate commercial suppliers Nitrogen Liquid nitrogen in conjunction with a heat exchanger is recommended because it is less expensive than compressed nitrogen and is usually of better quality If compressed nitrogen must be used the gas must be dry oil free and uncontaminated N
14. er to Chapter 4 for instructions on aligning the lamp Eye and Electrical Shock Hazards When operating the lamp module emits high intensity light which can damage eyes To avoid eye damage never operate the lamp module outside the instrument The lamp module contains components operating at high voltages To avoid electric shock never disassemble the lamp module Cary Eclipse Fluorescence Spectrophotometer User s Guide 43 Troubleshooting This page is intentionally left blank 44 Cary Eclipse Fluorescence Spectrophotometer User s Guide www agilent com In This Guide The guide describes the following Safety Practices and Hazards Introduction Installation Maintenance Troubleshooting Agilent Technologies 1999 2000 2011 2013 2015 Printed in Malaysia 10 15 8510175800 Issue 5 gh Agilent Technologies
15. ever use compressed nitrogen from a supplier who uses oil or water in the compression process Such processes invariably leave fine particles of oil suspended in the nitrogen which will form an oily deposit on optical surfaces Use nitrogen from a supplier who fills containers from immersion pumps lubricated with liquid nitrogen The instrument warranty will be void if damage is caused by the use of unsatisfactory nitrogen Pressure regulator and gauge Operating pressure is from 70 kPa 10 psi Always use an appropriate regulator and gauge to ensure that the nitrogen supply is consistently maintained at the correct pressure Cary Eclipse Fluorescence Spectrophotometer User s Guide 25 Installation Supply tubing Use clean flexible plastic Tygon polyvinyl chloride PVC or equivalent tubing of 6 mm 1 4 in internal diameter Never use rubber tubing because it may be treated internally with talc which can be blown into the optical system CAUTION Never use rubber tubing because it may be treated internally with talc which 26 can be blown into the optical system Gas manifold A gas manifold should be fitted with an inlet suitable for connection to the nitrogen supply system and an outlet suitable for connection to the spectrophotometer Flowmeter A flowmeter complete with control valves should be inserted in the system between the nitrogen supply system and the spectrophotometer The flowmeter should be capable
16. icable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a particular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or performance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the separate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcontract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer software as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or disclosure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government wil
17. inter manuals for their maintenance procedures and to the Cary Eclipse Help for the maintenance procedures for any Cary Eclipse accessories you ordered Cary Eclipse Fluorescence Spectrophotometer User s Guide 31 Maintenance and Spare Parts Cleaning Any spills in the sample compartment should be immediately wiped up The exterior surfaces of the Cary Eclipse spectrophotometer should be kept clean All cleaning should be done with a soft cloth If necessary this cloth can be dampened with water or a mild detergent Do not use organic solvents or abrasive cleaning agents Lamp module 32 This section describes how to replace the lamp module and realign the light beam Before changing the lamp module always disconnect the Cary Eclipse from the mains supply These instructions are also provided in the Cary Eclipse Help including videos to demonstrate the procedure To access the Cary Eclipse Help click Start gt All Programs gt Agilent gt Cary Eclipse gt Cary Eclipse Help Replacing Eye and Electrical Shock Hazards When operating the lamp module emits high intensity light which can damage eyes To avoid eye damage never operate the lamp module outside the instrument The lamp module contains components operating at high voltages To avoid electric shock NEVER disassemble the lamp module Cary Eclipse Fluorescence Spectrophotometer User s Guide Maintenance and Spare Parts To remove the lamp module 1 2
18. ith one of the same type and rating as indicated in Chapter 2 The fuses have a code marked on the cap for example T 3 15 AH 250 V This refers to the fuse characteristic T time lag F fast acting the current rating x amperes the braking capacity H heavy L low and the voltage rating y volts Fuse information on the rear of the instrument is the most up to date WARNING Electrical Shock and Fire Hazards To prevent reduced safety protection or unwanted fusing always ensure that the code on the fuse cap matches the information printed next to the fuse holders 38 Cary Eclipse Fluorescence Spectrophotometer User s Guide Maintenance and Spare Parts Checking To check a fuse 1 Disconnect the Cary Eclipse instrument from the mains power supply Open the hinged cap and pull out the fuse holder lower red section Both fuses are held in this fuse holder Check that each fuse is the correct type and that they are not damaged If necessary replace the fuse refer to Page 38 Place the fuses into the red fuse holder and push this red fuse holder into the main fuse holder and then lower the cap Reconnect the Cary Eclipse instrument to the mains power supply and turn on the instrument If a fuse repeatedly blows it may indicate other problems with the Cary Eclipse instrument Spare Parts Please see the Agilent website for a complete listing of spare parts
19. l receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met Cary Eclipse Fluorescence Spectrophotometer User s Guide Contents Contents 1 Safety Practices and Hazards 7 Lamp module 1 Ultraviolet radiation 8 Electrical hazards 8 Panels covers and modules 9 Other precautions 10 Warning and information symbols 11 Color coding 13 CE compliance 13 Electromagnetic compatibility 14 EN55011 CISPR11 14 ICES NMB 001 15 South Korean Class A EMC declaration 15 2 Introduction 17 Installation requirements 17 Documentation 17 Conventions 18 Specifications 18 Environmental 18 Weights and
20. lled e Check the connection of the main instrument cable USB GPIB HS attaching the computer to the instrument e Ensure the instrument has completed its initialization tests before you start the Cary Eclipse WinFLR software e Contact your local Agilent office Connect button instead of Start Problem When I start the Cary Eclipse WinFLR application I want to use the Start button but it has changed to a Connect button why Solution Check to see if you have any other Cary applications running If they are not collecting data click the Connect button to get control of the instrument 42 Cary Eclipse Fluorescence Spectrophotometer User s Guide Troubleshooting Instrument performance testing Problem The results of your instrument performance tests do not meet specifications the results obtained during factory testing are included in the packing crate with the instrument Solution Check the following e The sample compartment is empty e The cable connecting the instrument to the computer is correctly connected and the retaining screws are tightened e The lamp is pulsing during initialization This is indicated if the green power indicator on the front of the instrument flashes you should be able to hear the lamp and you should also be able to hear the monochromator and the filter wheel moving If the lamp is not pulsing you may have a hardware conflict e The lamp is correctly aligned ref
21. lower limit that is 20 lower than the current signal This will make the adjustment easier when observing the graph 8 Adjust the bottom left screw vertical adjustment labeled 1 in Figure 5 for maximum signal with the supplied 5 5 millimeter nut driver you may need to adjust the y axis scaling on the screen Cary Eclipse Fluorescence Spectrophotometer User s Guide 35 Maintenance and Spare Parts NOTE The brass adjustment screws for vertical and horizontal adjustment are clearly marked Figure 5 1 Vertical adjustment screw 2 Horizontal adjustment screw 9 Adjust the top right screw horizontal adjustment labeled 2 in Figure 5 for maximum signal see Figure 6 with the supplied 5 5 millimeter nut driver you may need to adjust the y axis scaling 36 Cary Eclipse Fluorescence Spectrophotometer User s Guide Maintenance and Spare Parts Figure 6 1 Maximum signal level 10 Click the Lamp tab 11 Click the Save conditions button to store instrument parameters NOTE Clicking the Save conditions button will store the current instrument parameters as well as the maximum reading Cary Eclipse Fluorescence Spectrophotometer User s Guide 37 Maintenance and Spare Parts Fuses The Cary Eclipse spectrophotometer contains two fuses that are located at the back of the instrument Replacing To replace a fuse 1 Disconnect the Cary Eclipse instrument from the power supply 2 Replace the blown fuse w
22. mpts as other programs may rely on these files to run 4 Repeat the process for the Cary Eclipse Help Software Status Bulletin Before proceeding further you should read the Software Status Bulletin document supplied with the software and the Agilent Cary Eclipse WinFLR Release Notes pdf located in the install directory These documents contain the latest release information and important notes Cary Eclipse Fluorescence Spectrophotometer User s Guide 29 Installation 30 This page is intentionally left blank Cary Eclipse Fluorescence Spectrophotometer User s Guide Maintenance and Spare Parts o oe e 4 Maintenance and Spare Parts Oe amp Cleaning 32 j j Lamp module 32 Fuses 38 Spare Parts 39 This chapter includes the maintenance procedures for the Cary Eclipse that may be carried out by an operator Any maintenance procedures not specifically mentioned in this chapter should be carried out only by Agilent trained Agilent qualified or Agilent authorized customer service representative Eye Hazard This instrument contains an intense light source Viewing of the light source either directly or via a reflecting surface will cause eye damage Operators and other unauthorized personnel must NEVER remove the black cover over the optics This section refers only to maintenance procedures for the Cary Eclipse fluorescence spectrophotometer You should refer to your computer and pr
23. ntenna 2 Move the device away from the radio or television 3 Plug the device into a different electrical outlet so that the device and the radio or television are on separate electrical circuits Make sure that all peripheral devices are also certified Make sure that appropriate cables are used to connect the device to peripheral equipment 6 Consult your equipment dealer Agilent Technologies or an experienced technician for assistance Cary Eclipse Fluorescence Spectrophotometer User s Guide Safety Practices and Hazards Changes or modifications not expressly approved by Agilent Technologies could void the user s authority to operate the equipment ICES NMB 001 This ISM device complies with Canadian ICES 001 Cet appareil ISM est conforme a la norme NMB 001 du Canada South Korean Class A EMC declaration Aw 717 88 ASEARA This equipment is Class A suitable for professional use and is for use in electromagnetic environments outside of the home 0 7S AHS A H AAPA SLBA H wep ES ABBE 0 ASF JIAN MEE ARAA AMA ASE AL Bao zs aud H Cary Eclipse Fluorescence Spectrophotometer User s Guide 15 Safety Practices and Hazards This page ts intentionally left blank 16 Cary Eclipse Fluorescence Spectrophotometer User s Guide Introduction g amp i amp e e 00 e e 2 Introduction a Installation requirements 17 i pr Documentation 17 Specifications 18 Inst
24. o that when used properly you have an accurate fast flexible and safe analytical system If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Information on safety practices appears throughout the documentation both hard copy and online provided with your instrument and accessories Before using the instrument or accessories you must thoroughly read these safety practices Observe all relevant safety practices at all times Lamp module Cary Eclipse Fluorescence Spectrophotometer User s Guide The lamp is enclosed in a self contained module This module contains components operating at high voltages To avoid electric shock NEVER disassemble the module Safety Practices and Hazards AA AN When operating the lamp module emits high intensity visible and ultraviolet UV radiation that can cause serious damage to eyes To avoid eye damage never operate the lamp outside the instrument Eye and Shock Hazard High voltage Xe flashlamp operating at 1260 kPa To avoid electric shock or eye damage NEVER disassemble the module or look down the hole La lampe Xe haut voltage fonctionne une pression de 1260 kpa Pour viter tout risque lectrique et des probl mes oculaires ne jamais ouvrir le module ou regarder l int rieur Ultraviolet radiation Hazardous ultraviolet UV radiation is emitted by the xenon flash lamp in the instrument
25. of monitoring flow rates between 0 and 30 liters per minute 64 cubic feet per hour Installing the software To install the Cary Eclipse WinFLR software 1 Log on to the instrument computer with Administrator privileges 2 Insert the software disk and click OK A User Account Control dialog box may appear Click Yes for Do you want to allow the following program to make changes to this computer If no installation screen appears run EclipseSetup exe from the CD directory Cary Eclipse Fluorescence Spectrophotometer User s Guide Installation 3 Follow the prompts on the screen until the Select Destination Location window appears Confirm the directory in which you would like to install the application Alternatively click Browse to choose a different location Click Next Agilent strongly recommends that the Agilent Cary Eclipse WinFLR folder and applications be installed in the recommended directory 4 The Folder Does Not Exist dialog box may appear Click Yes to create the folder 5 Follow the prompts on the screen until the Ready to Install window appears Click Install During the installation of the Net Framework and GPIB driver the computer may appear frozen and the Cancel button is unavailable This is correct The installation can take 3 to 5 minutes Do not try to exit the installation during this time 6 If prompted to complete installation of Cary WinFLR by restarting yo
26. sition the instrument on the bench to allow easy access to the power switch located on the front of the instrument Rear IEEE 488 GPIB Cary Eclipse system connection Cary Eclipse Fluorescence Spectrophotometer User s Guide 19 Introduction 20 Sample compartment e 9 pin D range connector low voltage AC DC e 15 pin D range connector low voltage AC DC e 25 pin D range connector low voltage AC DC Fuses T3 15 AH 250 V 5 x 20 mm IEC127 Sheet 5 For safety reasons any other internal fuse or circuit breaker is not operator accessible and should be replaced only by Agilent authorized personnel Fuse information on the rear of the instrument is the most up to date Nitrogen supply 70 kPa 10 psi Computer placement Locate the PC keyboard and mouse for ergonomically correct access Cary Eclipse Fluorescence Spectrophotometer User s Guide Installation oO E e e 0 e 3 Installation Pd a Single cell holder 22 m s ad j Other sample holders 25 a Purging 25 Installing the software 26 Installing the USB GPIB HS Converter 28 Registering the Cary Eclipse software 28 Uninstalling the software 29 Software Status Bulletin 29 The Agilent Cary Eclipse must be installed only by an Agilent trained or Agilent authorized representative Heavy Weight Hazard The Cary Eclipse weighs over 30 kg 66 Ib To avoid injury to personnel or damage to equipment always use two or more people
27. t that the product complies with the directives and also shows the EN standards to which the product was tested to demonstrate compliance Cary Eclipse Fluorescence Spectrophotometer User s Guide 13 Safety Practices and Hazards 14 Electromagnetic compatibility EN55011 CISPR11 Group 1 ISM equipment group 1 contains all ISM equipment in which there is intentionally generated and or used conductively coupled radio frequency energy which is necessary for the internal functioning of the equipment itself Class A equipment is equipment suitable for use in all establishments other than domestic and those directly connected to a low voltage power supply network which supplies buildings used for domestic purposes This device complies with the requirements of CISPR11 Group 1 Class A as radiation professional equipment Therefore there may be potential difficulties in ensuring electromagnetic compatibility in other environments due to conducted as well as radiated disturbances Operation is subject to the following two conditions 1 This device may not cause harmful interference 2 This device must accept any interference received including interference that may cause undesired operation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try one or more of the following measures 1 Relocate the radio or a
28. the instrument For further information refer to the Software Registration Help 3 Complete all the fields on the Customer Details page Click Next The Product Key is found on the cover of the Agilent Cary Eclipse software CD DVD case which was delivered with the instrument Complete all the fields on the Product Details page Click Next Complete all the fields on the Work Environment Details page Click Register A dialog appears stating Your Agilent Software Registration has been successful Cary Eclipse Fluorescence Spectrophotometer User s Guide Installation If your computer is not connected to the internet refer to the Software Registration Help for further information 7 The application will now open and you can collect your data To familiarize yourself with the Cary Eclipse WinFLR software browse the Help after installing the software To access the Help with a Cary Eclipse application open press F1 on your keyboard or click Start gt All Programs gt Agilent gt Cary Eclipse gt Cary Eclipse Help Uninstalling the software To uninstall the Cary Eclipse software 1 Click the Windows Start button Choose Settings Control Panel Add Remove programs Scroll until you find Cary Eclipse WinFLR 3 Click Add Remove and then Yes Follow the instructions on the screen During the uninstallation you may be prompted to remove certain drivers files Click No to these pro
29. upply voltage can create a fire hazard and a potentially serious shock hazard and could seriously damage the Cary Eclipse system accessories and any attached ancillary equipment Do not connect the Cary Eclipse your computer monitor printer or accessories to the mains power supply until you have made sure that they are correctly set for the mains power supply in the specific outlet in your laboratory to which the equipment will be connected Consult the manuals supplied with your computer monitor and printer for their specific voltage requirements Replace blown fuses with fuses of the size and rating as stipulated in the text adjacent to the fuse holder or in the manuals where listed Replace or repair faulty or frayed insulation on power cords WARNING Shock Hazard To avoid electrical shock this equipment must be disconnected from the mains supply before servicing Panels covers and modules You are permitted to remove the top colored cover to e Replace the lamp module e Adjust the lamp module Cary Eclipse Fluorescence Spectrophotometer User s Guide 9 Safety Practices and Hazards Any other panels or covers which are retained by screws on the spectrophotometer and accessories may be opened ONLY by Agilent trained Agilent qualified or Agilent approved service engineers Consult the manuals or product labels supplied with your computer monitor and printer to determine which parts are operator accessible
30. ur computer select No I will restart the computer later Click Finish 7 Remove the Cary Eclipse Software disk from the CD drive drive and insert the Cary Eclipse Help disk as prompted If using Windows 7 operating system a User Account Control dialog box will appear click Yes for Do you want to allow the following program to make changes to this computer If no installation screen appears run CaryFlirHelp setup exe from the CD directory 8 Follow the instructions on the screen to install the Help 9 When the status indicates Finished click Close 10 Remove the Help disk from the CD drive Cary Eclipse Fluorescence Spectrophotometer User s Guide 27 Installation 28 Installing the USB GPIB HS Converter To install a National Instruments USB GPIB HS converter Ensure that the Cary Eclipse WinFLR software has been installed Turn off the computer Connect one end of the converter to the instrument and the other end to the USB connection on the back of the computer Turn on the computer Registering the Cary Eclipse software To register the Cary Eclipse software 1 Click Start gt All Programs gt Agilent gt Cary Eclipse and then the desired application Alternatively double click the Cary Eclipse folder on the desktop The first time the Cary Eclipse software is opened a Software Registration dialog will appear Click Next Ensure the software registration is completed by the user of
31. www agilent com Cary Eclipse Fluorescence Spectrophotometer User s Guide 39 Maintenance and Spare Parts 40 This page is intentionally left blank Cary Eclipse Fluorescence Spectrophotometer User s Guide Troubleshooting KA Ka o e e amp 5 Troubleshooting No S Instrument offline 42 l a j Connect button instead of Start 42 Instrument performance testing 43 This chapter contains troubleshooting information to help you solve various problems you may encounter when setting up or using your Cary Eclipse If you are having problems with your software check the information in this chapter to see if there is a solution to your problem You may also find a solution to your problem in the e Troubleshooting section of the Help To view this click Start gt All Programs gt Agilent gt Cary Eclipse gt Cary Eclipse Help Click Troubleshooting and follow the links e Software Status Bulletin documents that were shipped with the Cary Eclipse WinFLR software If you still have not found the solution to your problem contact your local Agilent office or representative Cary Eclipse Fluorescence Spectrophotometer User s Guide 41 Troubleshooting Instrument offline Problem When I start the Cary WinFLR software the application reports that the instrument is Offline Solutions e Make sure the computer has been restarted after the Cary Eclipse WinFLR software is insta
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