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Agilent 1200 Series Variable Wavelength Detector G1314D /G1314E

1. 168 1200 Series VWD Service Manual Maintenance 12 3 Unscrew the thumb screws parallel and remove the flow cell If you want to maintain flow cell parts see Overview of Maintenance Parts on page 234 or the information provided with your flow cell 4 Replace the flow cell and fix the thumb screws 5 Replace the front cover Reconnect the inlet and outlet capillaries to the flow cell Next Steps 6 To check for leaks establish a flow and observe the flow cell outside of the cell compartment and all capillary connections Insert the flow cell 8 Perform Wavelength Verification Calibration on page 128 to check the correct positioning of the flow cell Replace the front cover 1200 Series VWD Service Manual 169 12 Maintenance Repairing the Flow Cells Parts required Description 1 For details on flow cell see Overview of Maintenance Parts on page 234 The shown cell parts will differ depending upon the flow cell type For detailed parts schematics refer to above mentioned pages Cell screw Conical springs Ring 1 PEEK Gasket 1 small hole Window Quartz Gasket 2 large hole Ring 2 PEEK RFID tag oN c 01 2 C65n2 Figure 66 Standard Flow Cell 1 Disassembling the Flow Cell a Unscrew the cell screw using a 4 mm hexagonal wrench b Remove the SST
2. Type acquired y axis range 1 E mAU auto y adjust Offset 10 ds m Method Settings Use method settings Apply to Method Cancel Help Figure 37 Edit Signal Plot Window The Online Plot Figure 38 on page 79 shows both the pump pressure and the detector absorbance signals Pressing Adjust the signals can be reset to the offset value and Balance would do a balance on the detector 78 1200 Series VWD Service Manual Using the Detector 5 Setting up an Analysis Online Plot Figure 38 Online Plot Window 13 If both baselines are stable set the Y range for the detector signal to 100 mAU If you start with a new UV lamp for the first time the lamp may show initial drift for some NOTE time burn in effect 1200 Series VWD Service Manual 79 5 Using the Detector vetting up an An 14 Select the menu item RunControl gt Sample Info and enter information about this ap Offline Data Analysis Resume Injection Run Sequence Pause Sequence Resume Sequence Stop Runj Injectj sequence QJ DGCALAS M Figure 39 plication Figure 39 on page 80 Press OK to leave this screen Sample Info System 2 xi Operator Name wolfgang Data File Path JEA CHEMSTATION 2 DATAS x Subdirectory ISOTEST Manual Filename iso 01 D Prefis Counter r Sample Parameters Location vial 1 blank run if no entr
3. 5 Lift the Main Power Supply out of the Powers Light Pipe interface At this stage you may replace the Main Power Supply or the Power Light Pipe 1200 Series VWD Service Manual 213 13 Repair Exchanging the Main Board and Power Supply 6 Insert the new Main Power Supply and fit the Power Light 8 Carefully insert the bottom foam not to break the thinner Pipe interface foam areas and press it down to the bottom 7 Check that the Power Switch operation is properly working 9 Fit the Temperature Sensor Cable the Lamp and Leak Sensor Cable in the bottom foam channel Next Steps 11 Fit the two screws that fix the Main Power Supply at the rear panel 12 Install the Optical Unit see Installing the Optical Unit on page 204 214 1200 Series VWD Service Manual Repair 13 Installation of Main Board For ESD information refer to Cautions and Warnings on page 180 Carefully slide insert the Main Board into the rear panel Assure correct positioning within the foam Refit the nut spring and washer to the analog output Refit the nuts to the Remote and RS 232 connector ci A c N Reconnect the Temperature Sensor Cable to connector TEMP SENS 1 on main board Reconnect the lamp connector to the Main Board Lamp Power Supply Reconnect the Heater Interface Cable to the Main Board Reconnect all cables from the Optical Unit to the Main Board
4. Modules with or without optional interface board Modules with LAN on board PRINT SENDMODULES Lxxx SER YYNNNNNNNN PRINT SENDMODULES Nxxx SER YYNNNNNNNN module identifier L module identifier N serial number embedded in single quotes serial number embedded in single quotes PMP ALS THM TCC VWD DAD MWD FLD RID DAD MWD VWD The first two characters are letters which should be capitalized The reply line will respond with RA 0000 SER followed by the module serial number you just entered 2 To change the type of the module use the following command print sendmodule Lxxx TYPE XXXXX or print sendmodule Nxxx TYPE XXXXX where xxx is the module type and XXXXX is the 5 character product number of the module e g G1314B 1200 Series VWD Service Manual 219 13 Repair Table 24 ChemStation Command Format Type Change Modules with or without optional interface board Modules with LAN on board PRINT SENDMODULES Lxxx TYPE XXXXX PRINT SENDMODULES Nxxx TYPE XXXXX module identifier L module identifier N product number XXXXX embedded in single quotes product number XXXXX embedded in single quotes PMP ALS THM TCC VWD DAD MWD FLD RID DAD MWD VWD Some Agilent 1100 1200 series modules require the correct main board version to match the type for example the SL modules 3 Power cycle the module Then restart the Agilent ChemStation If the serial number you have just enter
5. the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 1200 Series VWD Service Manual Introduction to the Variable Wavelength Detector 1 Early Maintenance Feedback EMF Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counter The detector module provides a EMF counter for the lamp The counter increments with lamp use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Dependig on the lamp type the counter can be reset to zero after the lamp is exchanged Table 2 Lamp Type Counter Reset Comment lamp
6. 1 Note the MAC Media Access Control address for further reference The MAC or hardware address of the LAN interfaces is a world wide unique identifier No other network device will have the same hardware address The MAC address can be found on a label at the rear of the module underneath the configuration switch 3 REMOTE s deze at CONFIG CAH RS 232 o LAN E Woe E COMPACT FLASH 61314 65080 part number of board B 01 22 0444 Revision Code Vendor Year and Week of assembly MAC 000001000001 MAC address Made in Germany Country of Origin Figure 9 Location of Configuration Switch and MAC Label 2 Connect the instrument s LAN interface to the PC network card using a crossover network cable point to point or a hub or switch using a standard LAN cable 46 1200 Series VWD Service Manual LAN Configuration 4 TCP IP parameter configuration To operate properly in a network environment the LAN interface must be configured with valid TCP IP network parameters These parameters are IP address Subnet Mask Default Gateway The TCP IP parameters can be configured by the following methods by automatically requesting the parameters from a network based BOOTP Server using the so
7. Remote RS232C CAN bus GPIB Connection ALS Thermostat G1330B Thermostat optional Serial Number Information Introduction to the Variable Wavelength Detector 1 Electrical Connections D CD 8 2a um l oo S CD E D A E 9 umm pP a quuin qum qum ey m Figure 5 Autosampler plus Thermostat Electrical Connections The serial number information on the instrument labels provide the following information CCYWWSSSSS Format CC country of manufacturing DE Germany JP Japan CN China YWW year and week of last major manufacturing change e g 820 could be week 20 of 1998 or 2008 SSSSS real serial number 1200 Series VWD Service Manual 19 1 Introduction to the Variable Wavelength Detector Instrument Layout 20 The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly
8. Temp Sensor Assembly includes board and cable loacted in the top foam see Heater and Fan on page 252 Plastic parts housing see Plastic Parts on page 255 Sheet metal parts see Sheet Metal Kit on page 254 Foam parts see Foam Parts on page 257 G1314 60009 Power and status light parts see Power and Status Light Pipes on page 258 1200 Series VWD Service Manual 249 15 Parts for Repairs Optical Unit Repairs in the optical unit are not possible due to critical alignments In case of problems the complete optical unit has to be replaced Table 35 Optical Unit Item Description Part Number 1 Optical unit with lamp G1314 60062 1 Optical unit exchange part without lamp G1314 69062 2 Deuterium lamp with RFID tag G1314 60101 3 Cable Lamp Power Supply G1314 60110 4 Flow Cell 5 Screw Kit Optical Unit includes 4 each G1314 67002 Screw Optical Cover Spring Washer 6 ID Tag Reader Cell G1315 60009 7 ID Tag Reader Lamp G1315 60009 8 Cover Optical G1315 60009 9 Damper Kit Optical Unit G1314 67001 250 1200 Series VWD Service Manual Parts for Repairs 15 Optical Unit e Figure 77 Optical Unit 1200 Series VWD Service Manual 251 15 Parts for Repairs Heater and Fan Table 36 Heater and Fan Item Description Part Number 1 Fan assembly 3160 1016 2 Heater Assembly 61314 60114 3 Heater Interface Board Kit includes Heater Board and 4 rivets G1314 67000 4 Heat
9. on page 226 shows the incorrect product number access to the module is possible as described in Recover with Agilent Diagnostic Software Type Only on page 226 If wrong firmware has been loaded in addition only the LMD Software can revert to correct product number as described in Recover with Agilent Diagnostic Software Type amp Firmware on page 226 comes up with an error unsupported module G1319B access to the module is possible via Service Mode as described in Recover with Instant Pilot on page 227 shows resident or unsupported module access to the module is possible via Tests as described in Recover with Control Module on page 228 shows the incorrect product number access to the module is possible as described in Recover with Agilent Diagnostic Software Type Only on page 226 1200 Series VWD Service Manual 225 13 Repair 226 Recover with Agilent Diagnostic Software Type Only The Diagnostic Softwares is used in CE mode If no LAN connection is possible use RS 232 The example uses G1315B as correct type 1 oF c N Open a connection to the module or via system The module will be listed with the wrong product number type Select Board Check and Change and press Start In the type field enter G1315B and press Apply Close the Agilent Diagnostic Software After a power cycle the module should show up with the correct product number type i
10. B 01 ZZ 0444 Revision Code Vendor Year and Week of assembly MAC 000001000001 MAC address Made in Germany Country of Origin Figure 8 Rear View of Detector 2 Check the setting of the DIP switch at the rear of the detector All switches should be in down position BOOTP mode If another boot mode is required refer to Link configuration selection on page 53 The detector was shipped with default configuration settings all switches down 3 Place the detector in the stack or on the bench in a horizontal position 4 Ensure the line power switch at the front of the detector is OFF 5 Connect the power cable to the power connector at the rear of the detector 6 Connect the CAN cable to other Agilent 1200 Series modules 1200 Series VWD Service Manual Installing the Detector 3 7 Connect the LAN cable e g from a Agilent ChemStation as controller to the detector s LAN connector In multi detector configurations the LAN of the Agilent G1315C D and G1365C D must be used due to its higher data load 8 Connect the analog cable s optional 9 Connect the APG remote cable optional for non Agilent 1200 Series instruments 10 Turn on power by pushing the button at the lower left hand side of the detector The status LED should be green Status indicator green yellow red J 7 S B y Line power switch D y with green light The detector is turned on when the line power sw
11. Press Setup and select the VWD signal Change the Y range to 10 to 300 mAU fit the Time Range to match Return to the PLOT window Press Start to start the run On completion of the run or when the run is stopped the test chromatogram is deactivated Test Chromatogram with Agilent ChemStation The test can be started via the command line To start the test PRINT SENDMODULES NVWD SIMU DFLT Reply RA 00000 SIMU DFLT Start the run On completion of the run or when the run is stopped the test chromatogram is deactivate 1200 Series VWD Service Manual 141 10 Test Functions for Service Diagnostic Signals 142 Overview of Diagnostic Signals The detector has several signals internal temperatures voltages and currents of lamps that can be used for diagnosing problems These can be baseline problems deriving from deuterium lamps wander drift problems due to temperature changes These signals can be used in addition to the normal baseline signal to determine whether correlation to temperature or voltage current of the lamp At introduction of the G1314D VWD and G1314E VWD SL Plus the diagnostic signals are available via the Agilent ChemStation B 03 02 SR1 using commands via the ChemStation command line The Agilent Diagnostic Software A 02 01 Service Release for G1314D E the Lab Advisor Software B 01 01 and the Instant Pilot G4208A do not provide this monitoring function Baseline Problem
12. To disconnect the instrument from line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off 1200 Series VWD Service Manual 297 17 Hardware Information 298 No accessible hardware fuse is needed because the main power supply is safe against any short circuits or overload conditions on the output lines When overload conditions occur the power supply turns off all output voltages Turning the line power off and on again resets the power supply to normal operation if the cause of the overload condition has been removed An over temperature sensor in the main power supply is used to turn off output voltages if the temperature exceeds the acceptable limit for example if the cooling fan of the instrument fails To reset the main power supply to normal operating conditions turn the instrument off wait until it is approximately at ambient temperature and turn the instrument on again The following table gives the specifications of the main power supply Table 47 Power Supply Specifications Standard Maximum power 160 VA 130W Continuous output Line Input 100 240 volts AC 10 line Wide ranging frequency of 50 60 Hz Pin 1 Power Fail error message Pin 2 AGND Pin3 15 VDC Pin 4 15 VDC Pin b PGND Pin 6 PGND Pin 7 24 VDC Pin 8 24 VDC Pin9 36 VDC not used Pin 10 36 VDC not used Pin 11 DGND Pin 12 5b VDC 1200 Series VWD Service M
13. cleaning or exchange cell windows done after repair Leak sensor drying If leak has occurred Check for leaks Leak handling system If broken or corroded Check for leaks replacement 164 1200 Series VWD Service Manual Maintenance 12 Exchanging a Lamp When Tools required Parts required Preparations If noise or drift exceeds application limits or lamp does not ignite Screwdriver POZI 1 PT3 Part number Description 1 G1314 60101 Deuterium lamp with RFID tag 1 or other Agilent recommended lamps Turn the lamp OFF If you want to use an Agilent DAD lamp instead of the VWD lamp you have to change the lamp settings in the VWD Configuration to the required lamp type This ensures that the DAD lamp s filament heating is operated like in the DAD The specification are based on the the standard RFID tag lamp 61314 60101 and may be not achieved when other lamp types or aged lamps are used Injury by touching hot lamp If the detector has been in use the lamp may he hot gt If so wait for lamp to cool down Injury by sharp metal edges gt Be careful when touching the RFI sheet metal at the rear of the fan There are sharp edges 1200 Series VWD Service Manual 165 12 Maintenance Exchanging a Lamp CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD gt To prevent accidental electrostatic discharge when coming into contact with components inside the instrum
14. tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1200 Series VWD Service Manual Contents Contents Introduction to the Variable Wavelength Detector 9 Introduction to the Detector 10 Optical System Overview 12 Electrical Connections 17 Instrument Layout 20 Early Maintenance Feedback EMF 21 Site Requirements and Specifications 23 Site Requirements 24 Physical Specifications 27 Performance Specifications 28 Installing the Detector 33 Unpacking the Detector 34 Optimizing the Stack Configuration 36 Installing the Detector 39 Flow Connections to the Detector 42 LAN Configuration 45 What you have to do first 46 TCP IP parameter configuration 47 Configuration Switches 48 Initialization mode selection 49 Link configuration selection 53 Automatic configuration with Bootp 54 Storing the settings permanently with Bootp 58 Manual Configuration 59 PC and Agilent ChemStation Setup 64 Using the Detector 67 Setting up an Analysis 68 Special Settings of the Detector 82 1200 Series VWD Service Manual Contents 6 How to optimize the detector 93 Optimizing the Detector Performance 94 Match the Flow Cell to the Column 9
15. 1 switch up x any position N A not assigned When selecting the mode TEST the LAN settings are Auto Negotiation amp Using Stored 294 1200 Series VWD Service Manual Hardware Information 17 LAN Settings For details on the LAN settings configuration refer to chapter LAN Configuration RS 232C Communication Settings The communication protocol used in this instrument supports only hardware handshake CTS RTS The RS 232C communication is always active parallel to the CAN and LAN communication For details see also RS 232C Serial in chapter Agilent 1100 1200 Series Interfaces on page 287 Per default the module will use 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable Boot Resident Settings Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a detector It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 45 Stay Resident Settings Mode Select SW 1 SW 2 SW 3 SW 4 SW 5 SW 6 SW7 SW 8 TEST BOOT 1 1 1 0 0 0 0 0 1200 Series VWD Service Manual 295 17 Hardware Information 296 Forced Cold Star
16. Repairs require the exchange of defective internal parts Removing the module from the stack opening up the covers and disassembling the module is required Special knowledge and training are needed to carry out these procedures Therefore these repairs should only be performed by trained Agilent service providers 1200 Series VWD Service Manual Maintenance and Repair 11 Warnings and Cautions Instrument is partially energized when switched off as long as the power cord is plugged in Risk of stroke and other personal injury Repair work at the detector can lead to personal injuries e g shock hazard when the detector cover is opened and the instrument is connected to power gt Never perform any adjustment maintenance or repair of the instrument with the top cover removed and with the power cord plugged in The security lever at the power input socket prevents that the detector cover is taken off when line power is still connected Never plug the power line back in when cover is removed Sharp metal edges Sharp edged parts of the equipment may cause injuries To prevent personal injury be careful when getting in contact with sharp metal areas Toxic and hazardous solvents The handling of solvents and reagents can hold health risks When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data
17. VWDS A Absorbance DAI 0609_S1WA_DEF_LO 2008 08 21 10 35 SaINV O105D mAU 04 pennan 03 0 2 0 1 T T 20 40 60 80 100 mi YWD3 F Board Temperature DAII 06098 9A DEF LC 2008 08 21 10 39 34 NY 0105 D degC 45 44 5 44 43 5 43 425 T T T T 20 40 60 80 100 miri VWD3 G Optical Unit Temperature DAII 0609 9YWA DEF LC 2008 08 21 10 39 34 NV 0105 D degC 38 5 38 375 37 36 5 36 T T T T T T T T T T T T T T T T T T T T T T T 20 40 60 80 100 mir YWD3 O Aux 1 DAII 0609_9 WA_DEF_LC 2008 08 21 10 39 34 NV 0105 D ents 30000 20000 10000 T T T T T T T T T T T T T T T T T T T T 20 40 60 80 100 min VWD3 P Aux 2 DAII 0609_9 WA_DEF_LC 2008 08 21 10 39 34 N V 0105 D ents 31000 30000 29000 28000 27000 26000 z r i i f r 20 40 60 80 100 mid Figure 63 Example plot from ChemStation 1200 Series VWD Service Manual 155 10 Test Functions for Service Diagno nals The same would happen in the opposite direction if the temperature drops that far that the heater duty cycle runs against the upper limit 95 at around 90 minutes The result is that the temperature in the detector optical and main board becomes unstable and the baseline starts to drift VWD3 A Absorbance DAIlI 0609 9YwA DEF LC 2008 08 21 10 39 34 NV 0110 D 0 01 NT 0 005 HV ePi Ph Z PXL a Pi i Nn t 0 005 0 01 T 40 60 80 100 min VWDS F Board Te
18. You need your own IP Subnet Mask and Gateway addresses Assure that the module configuration switch is set properly The setting should be either Bootp or Bootp amp Store see Initialization mode selection on page 49 Assure that the module connected to the network is powered off If the Agilent Bootp Service program is not already installed on your PC then install it from your Agilent ChemStation CD ROM located in folder NBootp The screens refer to version B 01 0x 1 The Agilent Bootp Service is placed in the start up group and automatically is started during the boot process of the PC 1200 Series VWD Service Manual LAN Configuration 4 Automatic configuration with Bootp 2 Open the Bootp Settings window Figure 15 on page 55 and enter the default settings for your setup Bootp Settings location of LogFile and TabFile Figure 15 Bootp Service Settings 3 Launch the Manager It will open the Bootp Manager screen see Figure 16 on page 55 This shows all network hardware that has been added initially empty Bootp Manager ix Hardware Address HostName IPAddress Comment SubnetMask Gateway Figure 16 Bootp Manager 1200 Series VWD Service Manual 55 4 LAN Configuration Automatic configuration with Bootp 4 Select Add to enter the enter the module specific information see Figure 17 on page 56 MAC address from label on the instrument host name
19. detector 76 parts for repair main assemblies 248 parts for maintenance accessory kit 235 cuvette holder 244 high pressure flow cell 242 leak parts 245 micro flow cell 238 overview 234 semimicro flow cell 240 standard flow cell 236 parts for repair foams 257 heater and fan 252 leak parts 256 optical unit 250 plastics 255 power and status 258 sheet metal kit 254 parts identification cables 259 parts and materials for maintenance 233 for repair 247 PC and Agilent ChemStation Setup 64 peakwidth settings 91 performance optimization 94 specifications 28 photodiode readings 126 photometric accuracy 97 physical specifications humidity 27 line voltage and frequency 27 operating temperature 27 power consumption 27 safety information 27 weight and dimensions 27 physical specifications 27 plastics parts 255 power supply description 297 parts 258 replacement 210 replacing 213 power considerations 24 consumption 27 cords 25 preparing the HPLC system 72 Radio Frequency Identification flow cellandlamp 10 radio interference 303 recalibration of wavelength 102 recover wrong type 224 remote cable 260 interface 290 repairs assembling main cover 231 cautions and warnings 180 cleaning the instrument 161 exchanging fan 190 exchanging status light pipe 209 313 Index for parts see parts for repair 247 heater assembly 195 heater interface cable 196 inside optical unit 199 installing foams and top cover 229
20. installing optical unit 204 introduction 157 leak sensor 207 main assemblies 185 main board 210 of internal parts 158 overview of simple repairs 164 power supply 210 213 removal of main board 211 removing optical unit 201 removing top cover and foam 182 replacing firmware 177 RFID tag reader 192 simple maintenance 158 simple repairs see maintenance 163 Temp Sensor 186 replacing parts see repairs 179 RFID 283 Radio Frequency Identification 10 tag reader replacement 192 RS 232C Communication settings 295 RS 232 cable 261 interface 289 run recovery no run data available in 122 run recovery automatic 87 manual 88 running the sample 81 314 S safety class 300 safety general information 300 300 symbols 302 sample info 80 sampling rate datarate 28 scanning 85 serial number information 19 serial number type using Agilent Diagnostic software 218 using ChemStation 219 using Control Mmodule 222 using Instant Pilot 221 serial number changing 217 setting up an analysis 68 settings analog output settings 89 peakwidth 91 setup of detector 76 sheet metal kit parts 254 signal diagnostic 102 148 plot 78 site requirements environment 26 power cords 25 power considerations 24 solvent information 306 sound emission 304 special setpoints 90 special settings 82 special interface 292 specifications performance 28 physical 27 spectra online 84 tools 29 stable baseline 74 stack
21. un as E uic L Figure 87 Temperature Control Air Flow 282 1200 Series VWD Service Manual Hardware Information 17 Lamp and Flow Cell RFID Tag The Agilent G1314D VWD and G1314E VWD SL Plus are equipped with a lamp flow cell identification system This allows to write and to read lamp flow cell specific information to and from the lamp flow cell identification tag see Flow Cell on page 13 and Lamp on page 14 Lamp information Flow cell information product number product number serial number serial number production date production date accumulated UV on time nominal path length of the cell actual UV lamp on time cell volume in nl number of ignitions maximum pressure in bar date of last intensity test date of last cell test This feature is available for special lamps and flow cells mentioned in chapter Table 26 on page 234 USB The USB connector is located left to the LAN connector It is not active yet and will be used for future enhancements 1200 Series VWD Service Manual 283 17 Hardware Information CompactFlash Card Slot The detector has a CompactFlash card slot G1314E VWD SL Plus only The G1314E VWD SL Plus is tested with the supplied CompactFlash card only Other CompactFlash cards may vary slightly and may not work correctly The CompactFlash card allows constant buffering of data spectra information so that in case of a busy ChemStati
22. 1050 1100 1200 TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 268 1200 Series VWD Service Manual Agilent 1100 1200 to HP 1090 LC or Signal Distribution Module Identifying Cables 16 Connector01046 60202 Pin HP 1090 Pin Agilent Signal Name Active 1100 1200 TTL 1 1 White Digital ground NC 2 Brown Prepare run Low 8 7 4 3 Gray Start Low 6 EL exe 7 4 Blue Shut down Low EM 8 5 Pink Not connected 1 NC 6 Yellow Power on High 3 7 Red Ready High 6 8 Green Stop Low NC 9 Black Start request Low Agilent 1100 1200 to General Purpose Connector01046 60201 Pin Universal Pin Agilent Signal Name Active 1100 1200 TTL 1 White Digital ground A Q 1 2 B oo Brown Prepare run Low oo es DB key 3 Gray Start Low oo poe HH su 4 Blue Shut down Low t is z 5 Pink Not connected oo rm 6 Yellow Power on High Ss Oo 15 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 1200 Series VWD Service Manual 269 16 Identifying Cables BCD Cables One end of these cables provides a 15 pin BCD connector to be connected t
23. 134 40 27 104 E Agilent Technologies G1314E DE81996062 gt lay help info splay current LAN settings t IP Address t Subnet Mask set Default Gateway exit shell Figure 23 Telnet Commands 60 1200 Series VWD Service Manual LAN Configuration 4 Table 11 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip lt X X X X gt sets new ip address sm lt X X X X gt sets new subnet mask gw lt X X X X gt sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows the style parameter value for example ip 134 40 27 230 Then press Enter where parameter refers to the configuration parameter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carriage return 5 Use the and press Enter to list the current settings c Telnet 134 40 27 104 Agilent Technologies G1314E DE81900002 CAN Status Page information about the LAN interface MAC Address 983 D38611BD MAC address initialization mode Init Mode Using Stored Initialization mode is Using Stored TCP IP Properties active IP Address z 134 40 27 104 255 255 248 0 Gateway 134 490 24 1 active TCP IP settings TCP IP status here ready no connections connected to PC with controller software here not connected Figure 24 Telnet Current
24. 1c eee uf Method and Run Control Samoaans ERN QJ DEMOCAL2 M Q DGALSTST M C3 QI DecaLAs M QI DGCALOQ1 M W DGCALOQ2 M QI DGCALOQ3 M QI DGCALOQ4 M QI pecaLogs m QJ DGcALOQS M DEFAULT D QJ DGCALPS M QI DGCHPTST M QI DGCPTEST M QI DGFLDTST M QI DGLEAKT M QI DGNOISE M QI DGNPTEST M QI DGTHMTST M QI pevwooc m E dala QJ ISOCRA M QJ ISOPLOT M E 1 1 GB free Figure 34 Loading Default LC Method 1200 Series VWD Service Manual QI BATCH M i fber LONO Sur Sumo no Detais 75 5 Using the Detector Setting up an Analysis 9 Click on the module icons Figure 35 on page 76 and open the Setup of these modules Figure 36 on page 77 shows the detector settings do not change the detector parameters at this time E Adata 76 DEFAULT D E 1 1 GB free Rf Set up YWD Signal fu Online Spectra B Not Ready Information Help Figure 35 Open the module menu 10 Enter the pump parameters mentioned under Table 14 on page 70 1200 Series VWD Service Manual Using the Detector 5 Setting up an Analysis Made 1 signal with individual wavelength setting stop and post time can be set if required gt peakwidth depends on the peaks in the chromatogram 254 see Peakwidth Settings on page 91 gt time table for programmable actions during the run bares es x gt Zero Offset Limits 1 to 99 in st
25. Below the box a reply line should then say Reply RA 0000 TYPE XXXXX XXXXX is what you just entered 1200 Series VWD Service Manual Repair 13 13 Power cycle the module Turn on should be normal In the Records screen the product column should indicate the module you just entered If an other User Interface is also connected start it now 1200 Series VWD Service Manual 223 13 Repair Recover Instructions Overview The following situations may come up where the instrument is no longer usable due to e an incorrect type product number entry after the replacement of a main board of the module load of wrong firmware based on the wrong type With firmware A 06 02 B 01 02 and above a wrong type cannot be entered The entry is checked against the board revision The wrong type product number could be incorrect but a valid 1100 1200 series module number incorrect and invalid 1100 1200 series module number any name Based on above the User Interfaces react differently Table 25 Recover From Wrong Type User Interface incorrect but valid type incorrect but valid type incorrect and invalid type Example Conditions correct type G1315B correct type G1315B correct type G1315B entered type G1314B entered type G1314B entered type G1319B plus wrong firmware from G1314B ChemStation shows the incorrect product does not show the module does not show the incorrect Recover with Agilent number NO acces
26. Clip for RFI ID tag Screw for Clip M2 2 4 5 mm long 79853 68739 79853 68734 G1314 65055 G1314 44010 0515 4780 1200 Series VWD Service Manual Parts and Materials for Maintenance 14 High Pressure Flow Cell 10 mm 14 pl Figure 74 High Pressure Flow Cell 1200 Series VWD Service Manual 243 14 Parts and Materials for Maintenance Cuvette Holder Table 32 Cuvette Holder Item Description Part Number Cuvette Holder G1314 60200 For information the use of the cuvette holder refer to Using the Cuvette Holder on page 173 Figure 75 Cuvette Holder 244 1200 Series VWD Service Manual Parts and Materials for Maintenance 14 eak Parts Leak Parts Table 33 Leak Parts Item Description Part Number 3 Leak funnel 5041 8388 4 Leak funnel holder 5041 8389 5 Clip 5041 8387 6 Corrugated tubing 120 mm lg re order 5 m 5062 2463 7 Corrugated tubing 1200 mm lg re order 5 m 5062 2463 Figure 76 Leak Parts 1200 Series VWD Service Manual 245 14 Parts and Materials for Maintenance Leak Parts 246 1200 Series VWD Service Manual 1200 Series VWD Service Manual e 6 ee 15 e Parts for Repairs 8 Overview of Main Assemblies 248 Optical Unit 250 Heaterand Fan 252 Sheet Metal Kit 254 Plastic Parts 255 Leak Parts 256 Foam Parts 257 Power and Status Light Pipes 258 This chapter provides information on parts for repair ORE Agilent Technologies 247 15 Parts for Rep
27. Figure 55 Holmium Oxide Test with Instant Pilot 1200 Series VWD Service Manual 131 9 Test Functions for User Holmium Oxide Test 132 1200 Series VWD Service Manual This chapter describes the detector s built in test functions for service 1200 Series VWD Service Manual 10 Test Functions for Service DAC Test 134 Grating Motor Test 136 Filter Motor Test 138 Test Chromatogram 140 Diagnostic Signals 142 RE Agilent Technologies 133 10 Test Functions for Service DAC Test 134 This test determines correct operation of the digital analog converter DAC The test applies an AC voltage 10 uV to the DC output of the DAC The DAC output is connected to the analog output connector at the rear of the detector The Dark Current Test is available in Agilent Diagnostic Software preferred tool Agilent Instant Pilot G4208A via Service Mode command Agilent ChemStation via command ADC1 A ADC CHANNEL A 61314A DACO0000 D 76 588 76 586 76 584 76 522 7658 4 T T T T v T pm T T 0 2 0 4 o6 os 1 12 1 4 16 18 minj Figure 56 DAC Test When to do the Test ifthe detector signal is noisy or missing Interpreting the Results The noise on the step should be less than 3 uV 1200 Series VWD Service Manual Test Functions for Service 10 DAC Test with Instant Pilot The test can be started via the command line To start the test TEST DAC 1 Reply
28. If the fan speed falls below two revolutions second for more than five seconds the error message is generated Probable cause Suggested actions 1 Fan cable disconnected Ensure the fan is connected correctly 2 Defective fan Exchange fan 3 Defective VWM board Exchange the VWM board Open Cover The top foam has been removed The sensor on the VWM board detects when the top foam is in place If the foam is removed during operation the lamp and grating drive power is switched off and the error message is generated Probable cause Suggested actions 1 Top foam removed Replace the foam 1200 Series VWD Service Manual 113 8 Error Information Detector Error Messages These errors are detector specific Lamp Current Missing The lamp anode current is missing The processor continually monitors the anode current drawn by the lamp during operation If the anode current falls below the lower current limit the error message is generated Probable cause Suggested actions 1 Lamp disconnected Ensure the lamp connector is seated firmly 2 Topfoam removed while lamp is on Replace the top foam and turn ON the lamp 3 Defective lamp or non Agilent lamp Exchange the lamp 4 Defective VWM board Exchange the VWM board 5 Defective power supply Exchange the power supply Lamp Voltage Missing The lamp anode voltage is missing The processor continually monitors the anode voltage across the lamp during operation If the anode
29. It will provide also the latest firmware of the Agilent 1200 Series modules for download 1200 Series VWD Service Manual 309 Index Index A absorbance Beer Lambert 96 accessory kit parts 235 Agilent ChemStation Setup 64 Diagnostic Software 106 Lab Advisor Software 106 oninternet 309 algea information 306 analog out put 29 analog cable 260 output range 89 output settings 89 signal output 290 APG remote connector 290 ASTM environmental conditions 26 automatic configuration with Bootp 54 auxiliary cable 260 B band width6 5nm 28 BCD cable 260 Beer Lambert absorbance 96 bench space 26 boards main board 279 310 Bootp amp Store 49 automatic configuration 54 initialization modes 49 service 54 storing the settings permanently 58 using default 51 using stored 50 Boot Resident settings 295 C cable analog 260 auxiliary 260 BCD 260 external contacts 261 GPIB 261 LAN 261 remote 260 RS 232 261 CAN interface 289 cautions and warnings 159 159 180 changing serial number and type 217 ChemStation changing serial number and type 219 recover type 227 chromatogram 71 cleaning the module 161 communications 29 CompactFlash card 284 configuration switch default settings 293 description and factory settings 293 location 48 configuration RFID tag lamp and cell 83 stack 36 temperature control 83 connectors main board 281 contaminated mirrors and grating 200 control and data
30. P address comment instrument name location subnet mask if different gateway if required Figure 17 Bootp Manager Enter your parameter 5 Press OK The parameter are added to the Bootp Manager see Figure 17 on page 56 and added to the TabFile see Figure 15 on page 55 56 1200 Series VWD Service Manual Bootp Manager x Hardware Address Host Name IP Address Comment SubnetMask Gatewa 0030d30a0838 WaADI1171 134 40 27 95 PP 24 255 255 248 0 0 0 0 0 LAN Configuration 4 Delete Figure 18 Bootp Manager check your entries 6 Press Exit Manager and OK to exit the Agilent Bootp Service 7 Now turn on the module wait about 30 60 seconds and view the LogFile see Figure below It should display the request from the module with the hardware MAC address 02 03 05 16 33 56 PM Status Status Status Status Status Status Figure 19 BOOTP Request received at outer most layer BOOTP Request received from hardware address 0030D30A0838 found 134 40 27 95 WADI1171 Host IP Address is 134 40 29 56 Reply to BOOTP Request has been sent BOOTP Request finished processing at outer most layer LogFile the detector has received the parameter When using this Bootp mode the parameters are not written into the non volatile memory of the module If you delete this Bootp Configuration the Bootp Manager will show up as shown in Figure 16 on page 55 Bo
31. Semi micro cell kit consisting of 61314 65056 two windows two 1 standard gaskets one 1 semi micro gasket and one 2 semi micro gasket 2 Conical springs pack of 10 79853 29100 3 Ring SST pack of 2 79853 22500 4 PTFE 1 standard gasket round hole i d 2 5 mm o d 8 mm 79853 68743 pack of 10 5 Quartz window pack of 2 79853 68742 6 Semi micro 1 gasket long hole 1 5 x 3 5 mm PTFE 7 Semi micro 2 gasket long hole 2 x 4 mm PTFE Inlet capillary 400 mm long 0 12 mm i d 5021 1823 8 Clip for RFID tag 61314 44010 9 Screw for Clip M2 2 4 5 mm long 0515 4780 The semi micro 1 and 2 gaskets items 6 and 7 look very similar Do not mix them up 240 1200 Series VWD Service Manual Parts and Materials for Maintenance 14 Semi micro Flow Cell 6 mm 5 pl Figure 73 Semi micro Flow Cell 1200 Series VWD Service Manual 241 14 Parts and Materials for Maintenance High Pressure Flow Cell 10 mm 14 pl 242 Table 31 High Pressure Flow Cell Assembly 10 mm 14 pl with RFID tag Item Description Part Number FSi wl n High pressure flow cell with RFID tag 10 mm 14 ul 400 bar G1314 60182 Capillary column detector SST 380 mm lg 0 17 i d one side not G1315 87311 assembled Cell screw Cell kit Agilent comprises 79853 27200 61314 65054 two windows two KAPTON gaskets and two PEEK rings Ring PEEK kit quantity 2 Window quartz kit quantity 2 Gasket kit KAPTON quantity 10
32. Table 6 Accessory Kit Contents Description Part Number Quantity Accessory kit CAN cable 0 5 m PEEK outlet capillary kit Fitting male PEEK Hex key 1 5 mm Hex key 4 mm Wrench open end 1 4 5 16 inch Wrench open end 4 mm G1314 68705 5181 1516 1 5062 8535 1 0100 1516 1 8710 2393 1 8710 2392 1 8710 0510 1 8710 1534 1 1200 Series VWD Service Manual 35 3 Installing the Detector Optimizing the Stack Configuration If your detector is part of a complete Agilent 1200 Series system you can ensure optimum performance by installing the following configuration This configuration optimizes the system flow path ensuring minimum delay volume 36 1200 Series VWD Service Manual Installing the Detector 3 Optimizing the Stack Configuration Solvent cabinet Vacuum degasser Local User Interface Autosampler Column compartment Detector Figure 6 Recommended Stack Configuration Front View 1200 Series VWD Service Manual 37 3 Installing the Detector Optimizing the Stack Configuration Analog Out Pump Remote cable CAN Bus cable to Local Controller CAN Bus cable Analog Out LAN to Controller Detector Figure 7 Recommended Stack Configuration Rear View 38 1200 Series VWD Service Manual Installing the Detector 3 Installing the Detector Parts required Part number Description 1 Detector 1 Power cord 1 LAN cable cross over or twisted pair netwo
33. Two 1 4 inch wrenches for capillary connections Parts required Description None 1 Remove the front cover 2 Use tissue to dry the leak sensor area 3 Observe the capillary connections and the flow cell area for leaks and correct if required 4 Replace the front cover Waste outlet eak sensor assembly Figure 67 Drying the Leak Sensor 1200 Series VWD Service Manual 175 12 Maintenance Replacing Leak Handling System Parts When If the parts are corroded or broken Tools required None Parts required Description For parts refer to Leak Parts on page 245 Remove the front cover to have access to the leak handling system Pull the leak funnel out of the leak funnel holder Pull the leak funnel with the tubing out of its location Insert the leak funnel with the tubing in its position 1 2 3 4 Replace the leak funnel and or the tubing 5 6 Insert the leak funnel into the leak funnel holder 7 Replace the front cover Leak funnel Leak funnel holder Leak tubing Figure 68 Replacing Waste Handling System Parts 176 1200 Series VWD Service Manual Maintenance 12 Replacing the Module s Firmware When Tools required Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The
34. analysis and e to use it as an instrument check to demonstrate that all modules of the system are correctly installed and connected It is not a test of the instrument performance Learn about special settings Before Using the System Solvent Information Observe recommendations on the use of solvents in chapter Solvents in the pump s reference manual Priming and Purging the System When the solvents have been exchanged or the pumping system has been turned off for a certain time for example overnight oxygen will re diffuse into the solvent channel between the solvent reservoir vacuum degasser when available in the system and the pump Solvents containing volatile ingredients will slightly lose these Therefore priming of the pumping system is required before starting an application 68 1200 Series VWD Service Manual Using the Detector 5 Table 12 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of the system When switching between reverse Isopropanol phase and normal phase both times Best solvent to flush air out of the system After an installation Ethanol or Methanol Alternative to Isopropanol second choice if no Isopropanol is available To clean the system when using Bidistilled water Best solvent to re dissolve buffer buffers crystals Bidistilled water After a solvent change Best solvent to re dissolve buf
35. at 265 nm The specification are based on the the standard RFID tag lamp 61314 60101 and may be not achieved when other lamp types or aged lamps are used ASTM drift tests require a temperature change below 2 C hour 3 6 F hour over one hour period Our published drift specification is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Performance tests should be done with a completely warmed up optical unit gt one hour ASTM measurements require that the detector should be turned on at least 24 hours before start of testing 1200 Series VWD Service Manual 31 2 Site Requirements and Specifications Performance Specifications 32 1200 Series VWD Service Manual 1200 Series VWD Service Manual 3 Installing the Detector Unpacking the Detector 34 Optimizing the Stack Configuration 36 Installing the Detector 39 Flow Connections to the Detector 42 This chapter describes the installation of the detector RE Agilent Technologies 33 3 Installing the Detector Unpacking the Detector CAUTION Condensation within the detector Condensation will damage the system electronics gt Do not store ship or use you
36. change the offset and the attenuation select VWD Signal gt More gt Special Setpoints WD Special Setpoints Instrument 2 xl Enable analysis when lamp is off Scan Range iso to aoo nm Restore Defaults Step nm Cancel Hep Figure 46 90 Special Setpoints Signal Polarity can be switched to negative if required Enable analysis when lamp is off if the VWD is not used in a dual detector setup lamp off the not ready condition is not stopping the analysis Scan Range Step Used for stop flow scanning see Scanning with the VWD on page 85 1200 Series VWD Service Manual Using the Detector 5 Peakwidth Settings Do not use peak width shorter than necessary see details below 1 To change the Peakwidth settings select Setup Detector Signals 2 In the section Peakwidth Responsetime click on the drop down list 3 Change the Peakwidth according to your needs g xj Peakwidth enables you to select the peak Pe coal Time width response time for your analysis The Pa Online Spectra NS ane peak width is defined as the width of a peak in 8 Not Ready Information Ee d cw 3 minutes at half the peak height Set the peak Help i dea a width to the narrowest expected peak in your Peakwidth Responsetime Posttime chromatogram The peak width sets the gt O1min 2s M Off E min optimum response time for your detector The 0 0012 min 0 03 i S
37. maximum was not found during wavelength calibration Calibration 0 Failed Zero order calibration failed Calibration 1 Failed 656 nm calibration failed During zero order and 656 nm calibration the detector searches for the intensity maximum If the maximum is not detected within the scan range the error message is generated Probable cause Suggested actions 1 Lamp is OFF Switch on the lamp 2 Incorrect flow cell installation Ensure the flow cell are installed correctly 3 Flow cell contamination or air bubbles Clean replace flow cell windows or remove air bubbles 4 Intensity too low Replace lamp 5 Current step value too far from maximum Enter a different calibration step value different scan range Repeat the calibration 6 Misaligned defective grating assembly Run the grating motor test to determine if the grating assembly is defective If defective replace the optical unit 7 Defective VWM board Exchange the VWM board 116 1200 Series VWD Service Manual Error Information 8 Holmium Oxide Test Failed The holmium oxide test in the detector has failed During the holmium test the detector moves the holmium filter into the light path and compares the measured absorbance maxima of the holmium oxide filter with expected maxima If the measured maxima are outside the limits the error message is generated Probable cause Suggested actions 1 Misaligned defective grating assembly Ensure the flow cell is inserted co
38. mode Using Stored and powers on the module again From now on he is able to establish the TCP IP connection to the module with the parameters obtained in that single Bootp cycle Bootp Active Server Parameter Non Volatile RAM Stored Parameter Figure 12 Bootp amp Store Principle Use the initialization mode Bootp amp Store carefully because writing to the non volatile memory takes time Therefore when the module shall obtain its parameters from a Bootp Server every time it is powered on the recommended initialization mode is Bootp Using Stored When initialization mode Using Stored is selected the parameters are taken from the non volatile memory of the module The TCP IP connection will be established using these parameters The parameters were configured previously by one of the described methods 1200 Series VWD Service Manual LAN Configuration 4 Non Volatile RAM Active Parameter Stored Parameter Figure 13 Using Stored Principle Using Default When Using Default is selected the factory default parameters are taken instead These parameters enable a TCP IP connection to the LAN interface without further configuration see Table 9 on page 51 Default Active Parameter Parameter Figure 14 Using Default Principle Using the default address in your local area network may result in network problems Take care and change it to a valid address immedi
39. of space on either side and approximately 8 cm 3 1 inches in the rear for air circulation and electric connections If the bench should carry a Agilent 1200 Series system make sure that the bench is designed to bear the weight of all modules The detector should be operated in a horizontal position Environment CAUTION Your detector will work within specifications at ambient temperatures and relative humidity as described in Table 3 on page 27 ASTM drift tests require a temperature change below 2 C hour 3 6 F hour measured over one hour period Our published drift specification refer also to Performance Specifications on page 28 is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Condensation within the module Condensation will damage the system electronics Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm up slowly to room temperature to avoid condensation 26 1200 Series VWD Service Manual Site Requirements and Specifica
40. oo N c Reinstall the Fan and reconnect it to the Main Board 1200 Series VWD Service Manual 215 13 Repair Next Steps 1 Replace the Top Foam see Removing the Top Cover and Top Foam on page 182 Replace the metal cover Replace the Closure and Front Panel Replace the detector in the system oF C N If a new main board has been installed enter the serial number as described in Changing the Type and Serial Number on page 217 perform a wavelength recalibration as described in Wavelength Verification Calibration on page 128 to enter the calibration parameter in the main board s memory 216 1200 Series VWD Service Manual Repair 13 Changing the Type and Serial Number Introduction When the main board has to be replaced the new board does not have a serial number For some modules e g pumps or auto samplers the type has to be changed multiple usage boards Use the information from the serial number plate of your module Keep in mind that the changes become active after a power cycle of the module the information from the serial number plate of the module is used the exact type product number is used If the type product number was entered incorrectly the module may become unusable Proceed to Overview on page 224 With firmware A 06 02 B 01 02 and above a wrong type cannot be entered The entry is checked against the board revision 1200 Series VWD Service Manual 217
41. rings using a pair of tweezers 170 1200 Series VWD Service Manual Maintenance 12 CAUTION Scratched window surfaces by tweezers Window surfaces can easily be scratched by using tweezers for removing the windows gt Do not use tweezers to remove windows c Use adhesive tape to remove the peek ring the window and the gasket d Repeat step 1 through step 3 for the other window keep the parts separate otherwise they could be mixed 2 Cleaning the Flow Cell Parts a Pour isopropanol into the cell hole and wipe clean with a piece of lint free cloth b Clean the windows with ethanol or methanol Dry it with a piece of lint free cloth Always use new gaskets 3 Reassembling the Flow Cell a Hold the flow cell cassette horizontally and place gasket in position Ensure both cell holes can be seen through the holes of gasket The semi micro 1 and 2 gaskets items 6 and 7 Semi micro Flow Cell 6 mm 5 yl on page 240 look very similar Do not mix them up b Place the window on gasket c Place the peek ring on the window d Insert the conical springs Make sure the conical springs point towards the window Otherwise tightening the cell screw might break the window e Screw the cell screw into the flow cell and tighten the screw 4 Repeat the procedure for the other cell side 5 Reconnect the capillaries 6 Perform a leak test If OK insert the flow cell 1200 Series VWD Service Manual 171 12 Maintenance R
42. safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1200 Series VWD Service Manual 301 18 Appendix Safety Symbols Table 48 Safety Symbols Symbol Description gt e The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up A WARNING alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the
43. terminal Table 43 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler 1200 Series VWD Service Manual 291 17 Hardware Information Special Interfaces Some 1100 1200 modules have module specific interfaces connectors They are described in the module documentation 292 1200 Series VWD Service Manual Hardware Information 17 Setting the 8 bit Configuration Switch All modules with on board LAN e g G1315 65C D G1314D E default is ALL switches DOWN best settings Bootp mod
44. the control module press System F5 then Records F4 Using the up down arrows make sure that the module is highlighted Press FW Update F5 then m This will display a box which says Update Enter Serial 4 Press Enter This will display the box labeled Serial Letters and numbers are created using the up and down arrows Into the box labeled Serial enter the 10 character serial number for the module When the 10 character serial number is entered press Enter to highlight the complete serial number Then press Done F6 Turn the module OFF then ON again The Records screen should display the correct serial number for this module If a Agilent ChemStation is also connected restart the Agilent ChemStation now as well To change the product number go to the System screen 8 9 Press Tests F3 and select the module and press Enter While in the Tests screen press m m m dot m 10 From the box now displayed select the Command and press Enter 11 Into the box labeled Nester instruction enter the command TYPE XXXXXX where XXXXXX is embedded in single quotes Letters and numbers are created using the up and down arrows XXXXX is the 5 character product number of the module being changed There must be a space between the word TYPE and the product number Some Agilent 1100 1200 series modules require the correct main board version to match the type for example the SL modules 12 Now press the Execute key
45. the main board and route 8 Replace and reconnect the fan see Exchanging the the sensor cables through the recesses on the optical Fan on page 190 step 2 cover 194 1200 Series VWD Service Manual Repair 13 Replacing the Heater Assembly Replacing the Heater Assembly When Tools required Parts required Preparations If Heater Assembly is defective The heater has to be removed for other replacements Screwdriver POZI 1 PT3 Part number Description 1 G1314 60114 Heater Assenbly Turn OFF the detector Injury by sharp metal edges gt Be careful when touching the RFI sheet metal at the rear of the fan There are sharp edges location 1 Unscrew the Heater Assembly and remove it from its 2 Insert the Heater Assembly bottom first and fix the Screw 3 Replace the front cover or continue with other repairs 1200 Series VWD Service Manual 195 13 Repair Replacing the Heater Interface Cable When If the Heater Assembly the Heater Interface Board or the Heater Cable is defective Tools required Screwdriver POZI 1 PT3 Parts required Part number Description 1 G1314 60114 Heater Assembly 1 G1314 67000 Heater Interface Board Kit includes 4 rivets 1 G1314 81010 Heater Cable Preparations Turn OFF the detector Disconnect the power cable Disconnect capillaries Remove detector from the system and place it on the working bench Remove the Front Panel the Cl
46. to illegal ambient conditions Suggested actions Ensure the connector to the detector main board is seated firmly Replace the temperature sensor Replace the main board Verify that the ambient conditions are within the allowed range Illegal Value From Air Inlet Temperature Sensor This temperature sensor located on the detector main board delivered a value outside the allowed range The parameter of this event equals the measured temperature in 1 100 centigrade As a result the temperature control is switched off Probable cause 1 The temperature sensor is defect 2 Detector is exposed to illegal ambient conditions Suggested actions Replace the temperature sensor Replace the main board Verify that the ambient conditions are within the allowed range 1200 Series VWD Service Manual Error Information 8 Heater Failed Every time the deuterium lamp or the tungsten lamp is switched on or off a heater self test is performed If the test fails an error event is created As a result the temperature control is switched off Probable cause Suggested actions 1 Defective connector or cable Ensure the connector to the detector main board is seated firmly 2 Defective heater Exchange heater assembly Heater Power At Limit The available power of the heater reached either the upper or lower limit This event is sent only once per run The parameter determines which limit has been hit 0 means
47. type 227 instrument layout 20 interface Agilent 1200 Series 287 analog signal output 290 CAN 289 GPIB 289 remote 290 RS 232 289 special 292 internet 309 introduction 10 to optical system 12 L Lab Advisor software 106 lamp and flow cell identification 283 lamp configuration 83 initial drift 79 intensity test 124 problems 142 type 28 types 142 312 LAN automatic configuration with Bootp 54 Bootp amp Store 49 Bootp service 54 Bootp 49 cable 261 configuration switches 48 configuration 45 initialization mode selection 49 link configuration selection 53 manual configuration with telnet 59 manual configuration 59 PC and Agilent ChemStation Setup 64 settings 295 storing the settings permanently 58 TCP IP parameter configuration 47 using default 51 using stored 50 what you have to do first 46 leak correcting 175 parts 245 256 replacing sensor 207 linearity 28 line power consumption 27 voltage and frequency 27 link configuration selection 53 LMD changing serial number and type 218 recover type and firmware 226 recovery type only 226 M MAC address 40 MAC address 46 main board functional diagram 280 layout and connectors 281 RFIDtag 283 main assemblies location 185 main board CompactFlash card 284 features 279 installation 215 overview 2 9 removal 211 replacement 210 temperature control 282 USB 283 maintenance cautions and warnings 159 correcting leaks 175 exchangin
48. 0 01 wt biphenyl 0 03 wt o terphenyl in methanol Conditions A single injection of the isocratic test standard is made under the conditions given in Table 14 on page 70 Table 14 Conditions Flow Stoptime Solvent Temperature Wavelength 1 5 ml minute 8 minutes 100 3096 water 70 Acetonitrile Ambient sample 254 nm 1200 Series VWD Service Manual Using the Detector 5 Table 14 Conditions Injection Volume 1 ul Column Temperature optional 25 0 C or ambient Typical Chromatogram A typical chromatogram for this analysis is shown in Figure 30 on page 71 The exact profile of the chromatogram will depend on the chromatographic conditions Variations in solvent quality column packing standard concentration and column temperature will all have a potential effect on peak retention and response DAD1 A Sig 254 4 Ref 500 100 04101410 140003 D mAU des yv en zi a Cm TL LL LC a min Figure 30 Typical Chromatogram with UV detector 1200 Series VWD Service Manual 71 5 Using the Detector Optimization of the System The settings used for this analysis are specific for this purpose For other applications the system can be optimized in various ways Please refer to the section Optimizing the Detector Performance on page 94 Preparing the HPLC System 1 Turn on the Agilent ChemStation PC and the monitor 2 Turn on the 1200 series
49. 0 cts measured 25507 Passed Avrg intens gt 160 000 cts measured 884724 Passed Finished Passed Y G Exit Used to determine the performance of the lamps and optics a 15 48 Figure 53 Checking the Photocurrent with the Instant Pilot 1200 Series VWD Service Manual 127 9 Test Functions for User Wavelength Verification Calibration Wavelength calibration of the detector is done using the zero order position and 656 nm emission line position of the deuterium lamp The calibration procedure involves two steps First the grating is calibrated on the zero order position The stepper motor step position where the zero order maximum is detected is stored in the detector Next the grating is calibrated against the deuterium emission line at 656 nm and the motor position at which the maximum occurs is stored in the detector In addition to the zero order and 656 nm alpha emission line calibration the beta emission line at 486 nm and the three holmium lines are used for the complete wavelength calibration process These holmium lines are at 360 8 nm 418 5 nm and 536 4 nm The wavelength verification calibration takes about 2 5 minutes and is disabled within the first 10 minutes after ignition of the lamp because initial drift may distort the measurement When the lamp is turned ON the 656 nm emission line position of the deuterium lamp is checked automatically The Wavelength Verification Calibra
50. 1200 Series VWD Service Manual Identifying Cables 16 START and STOP are connected via diodes to pin 3 of the 3394 connector Agilent 1100 1200 to 3396A Integrators Connector03394 60600 Pin 3394 Pin Agilent Signal Name Active 1100 1200 TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 15 s 3 3 Gray Start Low Fi o NC 4 Blue Shut down Low e o ee NC 5 Pink Not connected 1e NC 6 Yellow Power on High Q 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent 1100 1200 to 3396 Series II 3395A Integrators Use the cable part number 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1200 Series VWD Service Manual 267 16 Identifying Cables Agilent 1100 1200 to 3396 Series III 3395B Integrators Connector03396 61010 Pin 33XX Pin Agilent Signal Name Active 1100 1200 TTL 9 1 White Digital ground QO NC 2 Brown Prepare run Low 80 15 e 3 3 Gray Start Low i s NC 4 Blue Shut down Low e g ds NC 5 Pink Not connected 1e NC 6 Yellow Power on High Q 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent 1100 1200 to HP 1050 HP 1046A or Agilent 35900 A D Converters Connector5061 3378 Pin HP Pin Agilent Signal Name Active
51. 13 Repair Using the Agilent Diagnostic Software The Agilent Diagnostic Software must be configured in Service Mode to have access to the function Board Check and Change Close other user interfaces Start the Diagnostic Software 2 Select Tools on the left navigation panel 3 Select in the Tools Selection box Board Check and Change and press the button Start 4 Change the field Type and or Serial as required Some Agilent 1100 1200 series modules require the correct main board version to match the type for example the SL modules 5 Press the button Apply to complete the action A message The type was changed to XXXXXX Close this application and switch off and on the changed LC module 6 Close the Diagnostic Software 7 Power cycle the module 8 Restart the User Interface 218 1200 Series VWD Service Manual Repair 13 Using the Agilent ChemStation Module serial numbers are entered by typing specific commands on the command line at the bottom of the main user interface screen Turn the module on Start the Agilent ChemStation 1 To enter a module serial number type the following command into the command line print sendmodule Lxxx ser YYNNNNNNNN or print sendmodule Nxxx ser YYNNNNNNNN where xxx is the module type YY is country code in capital letters and NNNNNNNN the 8 character serial number of the module in question Table 23 ChemStation Command Format Serial Number Change
52. 5 Set the Detector Parameters 99 7 Troubleshooting and Diagnostics 101 Overview of the Detector s Indicators and Test Functions 102 Status Indicators 103 Available Tests versus Interfaces 105 Agilent Diagnostic Software 106 8 Error Information 107 What Are Error Messages 108 General Error Messages 109 Detector Error Messages 114 9 TestFunctions for User 123 Intensity Test 124 Checking the Photocurrent 126 Wavelength Verification Calibration 128 Holmium Oxide Test 130 10 Test Functions for Service 133 DAC Test 134 Grating Motor Test 136 Filter Motor Test 138 Test Chromatogram 140 Diagnostic Signals 142 11 Maintenance and Repair 157 Introduction to Maintenance and Repair 158 Warnings and Cautions 159 Cleaning the Detector 161 Using the ESD Strap 162 1200 Series VWD Service Manual Contents 12 Maintenance 163 Overview of Maintenance 164 ExchangingaLamp 165 Exchanging a Flow Cell 168 Repairing the Flow Cells 170 Using the Cuvette Holder 173 Correcting Leaks 175 Replacing Leak Handling System Parts 176 Replacing the Module s Firmware 177 13 Repair 179 Cautions and Warnings 180 Removing the Top Cover and Top Foam 182 Locate the Main Assemblies 185 Replacing the Temperature Sensor 186 Exchanging the Fan 190 Replacing the RFID Tag Reader Lamp or Flow Cell 192 Replacing the Heater Assembly 195 Replacing the Heater Interface Cable 196 Repairs in the Optical Unit 199 Removing the O
53. 5900 60750 General purpose spade lugs 01046 60105 Remote cables 3390 integrator 01046 60203 3392 3 integrators 01046 60206 3394 integrator 01046 60210 3396A Series I integrator 03394 60600 3396 Series Il 3395A integrator see details in section Remote Cables on page 265 3396 Series III 3395B integrator 03396 61010 HP 1050 modules HP 1046A FLD 5061 3378 HP 1046A FLD 5061 3378 Agilent 35900A A D converter 5061 3378 HP 1040 diode array detector 01046 60202 HP 1090 liquid chromatographs 01046 60202 Signal distribution module 01046 60202 BCD cables 3396 integrator 03396 60560 General purpose spade Lugs G1351 81600 Auxiliary Agilent 1100 Series vacuum degasser G1322 61600 260 1200 Series VWD Service Manual Identifying Cables 16 Type Description Part Number CAN cables Agilent 1100 1200 module to module 0 5m Ig 5181 1516 Agilent 1100 1200 module to module 1m Ig 5181 1519 External Agilent 1100 1200 Series interface board to general G1103 61611 contacts purpose GPIB cable Agilent 1100 1200 module to ChemStation 1 m 10833A Agilent 1100 1200 module to ChemStation 2 m 10833B RS 232 cable Agilent 1100 1200 module to a computer 34398A This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter LAN cable Twisted pair cross over LAN cable shielded 3m long for 5023 0203 point to point connection Twisted pair cross over LAN cable shielded 7m long for 5023 0202 point to point
54. 8535 Fitting male PEEK quantity 1 0100 1516 Hex key 1 5 mm 8710 2393 Hex key 4 mm 8710 2392 Wrench open end 1 4 5 16 inch 8710 0510 Wrench open end 4 mm 8710 1534 1200 Series VWD Service Manual 235 14 Parts and Materials for Maintenance Standard Flow Cell 10 mm 14 pl Table 28 Standard Flow Cell Assembly 10 mm 14 pl with RFID tag Item Description Part Number Standard Flow Cell with RFID tag 10 mm 14 ul 40 bar 61314 60186 Capillary column detector PEEK 600 mm lg 0 17 mm i d 1 16 5062 8522 inch o d Cell Repair Kit includes 2x Gasket 1 2x Gasket 2 2x Window 81314 65061 Quartz 1 Cell screw kit quantity 2 61314 65062 2 Conical spring kit quantity 10 79853 29100 3 Ring 1 PEEK kit quantity 2 G1314 65065 4 Gasket 1 small hole i d 1 mm o d 7 9 mm KAPTON 61314 65063 quantity 10 5 Window quartz kit quantity 2 79853 68742 6 Gasket 2 large hole i d 2 4 mm o d 7 9 mm KAPTON G1314 65064 quantity 10 7 Ring 2 PEEK kit quantity 2 G1314 65066 8 Clip for RFI ID tag G1314 44010 9 Screw for Clip M2 2 4 5 mm long 0515 4780 236 1200 Series VWD Service Manual Parts and Materials for Maintenance 14 Standard Flow Cell 10 mm 14 pl Figure 71 Standard Flow Cell 1200 Series VWD Service Manual 237 14 Parts and Materials for Maintenance Micro Flow Cell 3 mm 2 pl Table 29 Micro Flow Cell Assembly 3 mm 2 ul with RFID tag Item Description Part Number
55. AJOJN Micro flow cell with RFID tag 3 mm 2 ul 120 bar Capillary column detector SST 400 mm lg 0 12 mm i d Window screw Spring washers pack of 10 Compression washer Window holder Quartz window Gasket BACK round hole 1 8 mm Teflon outlet side Gasket FRONT round hole 1 3 mm Teflon inlet side Clip for RFI ID tag Screw for Clip M2 2 4 5 mm long Capillary IN 0 12 mm 310 mm lg Capillary OUT 0 17 mm 120 mm lg Cell repair kit semi micro includes window screw kit 4 mm kexagonal wrench and seal kits Seal kit BACK for semi micro flow cell qty 12 of item 6 Seal kit FRONT for semi micro flow cell qty 12 of item 7 Window screw kit includes 2 quartz windows 2 compression washers 2 window holders 2 window screws and 10 spring washers G1314 60187 5021 1823 79883 22402 5062 8553 79883 28801 79883 22301 1000 0488 see kits below see kits below G1314 44010 0515 4780 G1314 87301 G1314 87302 G1315 68713 79883 68702 G1315 68710 79883 68703 238 1200 Series VWD Service Manual Parts and Materials for Maintenance 14 Micro Flow Cell 3 mm 2 pl Figure 72 Micro Flow Cell 1200 Series VWD Service Manual 239 14 Parts and Materials for Maintenance Semi micro Flow Cell 6 mm 5 pl Table 30 Semi micro Flow Cell Assembly 6 mm 5 ul with RFID tag Item Description Part Number Semi micro flow cell assembly with RFID tag 6 mm 5 ul 40 bar G1314 60183 1 Cell screw 79853 27200
56. Agilent 1200 Series Variable Wavelength Detector G1314D G1314E SL Plus Service Manual XE Agilent Technologies Notices Agilent Technologies Inc 2008 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61314 90130 Edition 09 08 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Research Use Only Not for use in Diagnostic Procedures Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with thes
57. D SL button 4 Scroll down to the LAN settings Configure VWD SL EK Value Stays off at power on gt ks m Temperature Control ON Cell Tag Use only cells with RFID tag Uv Lamp Type Auto detect from RFID tag info LAN Subnet Mask 255 255 248 0 LAN Def Gateway 134 40 24 1 Analog Out OV 1V output range LAN IP 134 40 27 104 Exit lum sen 12 11 System I Controller I VWD SL I Figure 27 Instant Pilot LAN Configuration I 5 Press the Edit button perform the required changes and press the Done button 6 Leave the screen by pressing the Exit button 1200 Series VWD Service Manual 63 4 LAN Configuration PC and Agilent ChemStation Setup PC and Agilent ChemStation Setup PC Setup for Local Configuration This procedure describes the change of the TCP IP settings on your PC to match the module s default parameters in a local configuration see also Figure 14 on page 51 FIXED IP address automatic IP address via DHCP requires special IT setup of the network Figure 28 Changing the TCP IP settings of the PC 64 1200 Series VWD Service Manual LAN Configuration Agilent ChemStation Setup 1 Start the Configuration Editor of the Agilent ChemStation Select Instrument LC1100 01 Device Configuration Instrument Type Modular 3D LC Modules 1090 LC System LC 3D Data Analysis only ucrT5 LC Data Analysis onl UN TT I ump s
58. DAD and G1365C D MWD This interface allows the control of the module system via a connected PC with the appropriate control software e g Agilent ChemStation GPIB This interface is not available in all modules and may be removed from the modules in future The GPIB connector is used to connect the module with a computer The address and control switches next to the GPIB connector determine the GPIB address of your module The switches are preset to a default address and recognized by the operating software from Agilent Technologies RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module next to the GPIB connector 1200 Series VWD Service Manual 289 17 Hardware Information 290 The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Instrument PC DCD 1 1 DCD RX 2 2 RX TX 3 A 3 TX DTR 4 P 4 DTR GND 5 y 5 GND DSR 6 6 DSR RTS 7 V 7 RTS cts 8 slias RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 89 RS 232 Cable Analog Signal Output The analog signal output e g detector signal or pump pressure signal can be distributed to a recording device For details refer to the description of the main board of the module APG Remote The APG Rem
59. HPLC modules 3 Start the Agilent ChemStation software If the pump autosampler thermostatted column compartment and detector are found the ChemStation screen should look like shown in the figure below The System status is red Not Ready System status Method and Run Control ntral Start 5 00 DEFAULT D LastRun NRT 0 ction oen ie ig sequence oer ics EME wan i EB O no Details hd E Adata E 1 1 GB free 5 10 Online plot window details window Figure 31 Initial ChemStation screen Method and Run Control 72 1200 Series VWD Service Manual Using the Detector 5 4 Turn on the detector lamp pump and autosampler by clicking the System On button or the buttons below the module icons on the graphical user interface GUI After some time the pump thermostatted column compartment and detector module will turn to green Method and Run Control as E O nen ces eic EME sumo SiO c 2 en Jot DEFAULT D 20 i no Details m PE 4 System 2 L ed L System 2 xj System 2 x ho Turnon all pumps bo j Turn on the thermostat 2 J Turn on the U v lamp of the vWwD Module bo o o tastRun D 100 ocn tgs A l E DEFAULT D no Details E E Adata E 1 1 GB free E i 5 10 min Figure 32 Turning on the HPLC Module 1200 Series VWD Servic
60. IE E af peak detector ignores any peaks that are Tin gt 0 0025 min 0 06 s considerably narrower or wider than the peak Bal Insert width setting The response time is the time alance can mI between 10 and 90 of the output signal in X0 1 min s response to an input step function When the gt O2min 4s Lm j gt 04min Bs A All spectrum storage option is selected then Eo spectra are acquired continuously depending on the setting of the peak width The time specified by the peak width is used as a factor Table C Graphic in the acquisition of spectra The acquisition time for one spectrum is slightly less than the OK Cancel i zs ES EN Maez peak width divided by 8 see Table 16 on Figure 47 Peakwidth Setting page 92 and Table 17 on page 92 Limits When you set the peak width in minutes the corresponding response time is set automatically and the appropriate data rate for signal acquisition is selected as shown in Table 16 on page 92 and Table 17 on page 92 1200 Series VWD Service Manual 91 5 Usingthe Detector Table 16 Peak Width Response Time Data Rate G1314D VWD peakwidth at half heigth min Response Time s Data Rate Hz 0 005 0 12 20 20 005 0 12 20 20 01 0 25 20 20 025 0 5 20 20 05 1 0 10 20 10 2 0 5 20 20 4 0 2 5 gt 0 40 8 0 1 25 Table 17 Peak Width Response Time Data Rate G1314E VWD SL Plus peakwidth at half heigth min Response Time s D
61. Information i ui Take current method Error Method take error method or current method in case of an error Analog Output Range can be set to either 100 mV or 1 M Analog Dutput B h du EN V full scale see Analog Output Settings on page 89 cet INE oa Automatic Turn On lamps can be programmed Tum lamp on detector must be on for this Help online help Automatic Tum On Tum lamp on at dd mmyyyy 21 05 2008 Cancel Help Figure 41 Detector control settings 82 1200 Series VWD Service Manual Using the Detector 5 Configuration Settings Instrument Method Sequence View Abort Help Temperature Control the optical unit is kept on constant Set up YWD Signals a temperature some degrees above ambient and improves the baseline stability in unstable environments See also Control note below Snapshot Configuration UV lamp tag Automatic mode for Agilent lamps with RFIDtags If no RFID tag lamp is used detector icon will Sample Scan ratom on Blank Scan become gray lamp tag not ready and analysis is disabled System Off vw Configuration Instrument2 x The specifications are based on the lamp with RFID tag remped esum Use UV lamp anyway here you can select non RFID tag lamps such as of type VWD lor DAD have different Temperature control on heating mechanismn Temperature control off The correct
62. No No No A Yes No Yes G1322A DEG No No No No No No Yes AUX G1379A DEG No No No No Yes No No AUX G4240A CHIPCUBE 2 Yes No No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED 288 CAN connectors as interface to other Agilent 1200 Series modules GPIB connector as interface to the Agilent ChemStation RS 232C as interface to a computer REMOTE connector as interface to other Agilent products analog output connector s for signal output and interface slot for specific interfacing external contacts BCD LAN and so on For identification and location of the connectors see the module manual 1200 Series VWD Service Manual Hardware Information 17 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement If a Agilent 1100 1200 series detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler LAN The 1100 1200 modules have either an interface slot for an LAN card e g Agilent G1369A LAN Interface or they have an on board LAN interface e g detectors G1315C D
63. RA 00000 TEST DAC 1 To stop the test TEST DAC 0 Reply RA 00000 TEST DAC 0 DAC Test with Agilent ChemStation The test can be turned on off via the command line To start the test PRINT SENDMODULES NVWD TEST DAC 1 Reply RA 00000 TEST DAC 1 To stop the test PRINT SENDMODULES NVWD TEST DAC 0 Reply RA 00000 TEST DAC 0 DAC Test Fails Probable cause Suggested actions 1 Defective VWM board Exchange the VWM board 1200 Series VWD Service Manual 135 10 Test Functions for Service Grating Motor Test This test moves the grating motor to the end position and displays the motor position step difference This checks the movement of the grating drive mechanism The Grating Motor Test is available in Agilent Diagnostic Software preferred tool Agilent Instant Pilot G4208A via Service Mode command Agilent ChemStation via command When to do the Test ifrecalibration cannot be done successfully or ifthe holmium test fails Interpreting the Results There result is either succeeded or failed Grating Motor Test with Instant Pilot The test can be started via the command line To start the test TEST GRAT Reply RA 00000 TEST GRAT Test succeeded when passed 136 1200 Series VWD Service Manual Test Functions for Service 10 Grating Motor Test with Agilent ChemStation The test can be started via the command line To start the test PRINT SENDMODULES NV
64. RS 232 cable kit 9 to 9 pin female and one adapter 9 pin male 25 pin female Suited for instrument to PC Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter Use the cable and adapter to connect Agilent Technologies instruments with 9 pin male RS 232 connectors to most PCs or printers 24542U G1530 60600 34398A 5181 1529 34398A 1200 Series VWD Service Manual 215 16 Identifying Cables RS 232 Cables 276 1200 Series VWD Service Manual 1200 Series VWD Service Manual 17 Hardware Information Optical System Components 278 Detector Main Board 279 Firmware Description 285 Agilent 1100 1200 Series Interfaces 287 Setting the 8 bit Configuration Switch 293 Main Power Supply Assembly Standard 297 This chapter describes the detector in more detail on hardware and electronics ORE Agilent Technologies 277 17 Hardware Information Optical System Components The figure below shows the main components of the optical unit For details refer to Optical System Overview on page 12 Slit Assembly Grating Assembly Filter Assembly Mirror M1 Source Lens l Lamp Photo Diode and i ADC Board SAMPLE Mirror M2 Flow Cell Photo Diode and Beam Splitte
65. Run In Progress information until the data is recovered from disk 1200 Series VWD Service Manual 87 5 Using the Detector Special Settings of the Detector Manual Run Recovery in case of permanent communication failures DAD Run Recovery G1315C see Note below 5 Not Ready Information Start a recover DAD Run Recovery G1315C After a recover DAD Run Recovery G1315C NOTE When during recovery an error Method Sequence stopped appears the instrument logbook shows an entry No Run data available in device 88 1200 Series VWD Service Manual Using the Detector 5 Special Settings of the Detector Analog Output Settings 1 To change the Output Range of the analog outputs select VWD Control 2 To change the offset and the attenuation select VWD Signal gt More WD Control System 2 go ey Analog Output Range can be set to either 100 mV or 1 V full scale Zero ffset can be set to either 100 mV or 1 V full scale Attenuation Limits 0 98 to 4000 mAU at discrete a values for either 100 mV or 1 V full scale te cad mmy Time chhommi ss Figure 44 Analog Output Settings i D Signal System 2 Figure 45 Analog Output Settings 3 Change the values if required 1200 Series VWD Service Manual 89 5 Using the Detector Special Setpoints QE Set up VwD Signal a Control Fu Online Spectra 8 Not Ready Information Help 1 To
66. Steps 1200 Series VWD Service Manual Repair 13 Exchanging the Main Board and Power Supply Removal of Main Board NOTE For ESD information refer to Cautions and Warnings on page 180 1 Unscrew the nut spring washer from the analog output 14 mm wrench 2 Unscrew the nuts from the Remote and RS 232 connector 5 mm wrench 3 Unscrew the screw right to the LAN connector REMOTE ANALOG Screws for Power Supply Screw for Main Board Figure 70 Rear of detector 4 Disconnect and remove the fan 1200 Series VWD Service Manual 211 13 Repair Exchanging the Main Board and Power Supply 5 Disconnect the lamp connector from the Main Board Lamp Power Supply by pressing the release button at the lower side of the connector 6 Disconnect the Temperature Sensor from the Main Board TEMP SENS1 7 Remove the Main Board by sliding it out of the rear panel 8 Place the Main Board on an ESD kit 212 1200 Series VWD Service Manual Repair 13 Replacing Main Power Supply Power Light Pipe 1 Remove the Optical Unit see Removing the Optical 4 Carefully lift and slide out the bottom foam not to break Unit on page 201 the thinner foam areas at the Main Power Supply until you have access to the power light pipe 2 Unscrew the two screws that fix the Main Power Supply at the rear panel 3 Pull the Lamp Cable out of the bottom foam channel
67. Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 272 1200 Series VWD Service Manual Identifying Cables 16 CAN LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent 1200 Series module s CAN or LAN connectors CAN Cables Agilent 1200 module to module 0 5 m 5181 1516 Agilent 1200 module to module 1 m 5181 1519 Agilent 1200 module to control module G1323 81600 LAN Cables Description Part number Cross over network cable shielded 3 m long for point to 5023 0203 point connection Twisted pair network cable shielded 7 m long for hub 5023 0202 connections 1200 Series VWD Service Manual 273 16 Identifying Cables Auxiliary Cables One end of this cable provides a modular plug to be connected to the Agilent 1100 Series vacuum degasser The other end is for general purpose Agilent 1100 Series Degasser to general purposes ConnectorG1322 81600 Color Pin Agilent Signal Name 1100 White 1 Ground ca Brown 2 Pressure signal a Green 3 SS Yellow 4 Grey 5 DC 5VIN Pink 6 Vent 274 1200 Series VWD Service Manual RS 232 Cables Identifying Cables 16 Description Part number RS 232 cable instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters
68. WD TEST GRAT Reply RA 00000 TEST GRAT During test the instrument shows Prerun Selftest After test the instrument shows Ready or Error Grating Motor Test Failed Probable cause Suggested actions 1 Probable Cause Exchange Optical Unit 2 Defective main board Exchange the VWM board 1200 Series VWD Service Manual 137 10 Test Functions for Service Filter Motor Test This control function is used to check the motion of second order cut off filter The cut off filter returns to original position at the end of this control function The Filter Motor Test is available in Agilent Diagnostic Software preferred tool Agilent Instant Pilot G4208A via Service Mode command Agilent ChemStation via command When to do the Test ifthe holmium test fails Interpreting the Results There result is either succeeded or failed Filter Motor Test with Instant Pilot The test can be started via the command line To start the test TEST FILT Reply RA 00000 TEST FILT Test succeeded when passed 138 1200 Series VWD Service Manual Test Functions for Service 10 Filter Motor Test with Agilent ChemStation The test can be started via the command line To start the test PRINT SENDMODULES NVWD TEST FILT Reply RA 00000 TEST FILT During test the instrument shows Prerun Selftest After test the instrument shows Ready or Error Filter Motor Test Failed Probable cause Sugg
69. ace Refer to the Service Manual for details Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components Refer to the User Interface and or the Service Manual for details Wavelength Verification Recalibration Wavelength recalibration is recommended after repair of internal components and on a regular basis to ensure correct operation of the detector The detector uses the deuterium alpha and beta emission lines for wavelength calibration see Wavelength Verification Calibration on page 128 Diagnostic Signals The detector has several signals internal temperatures voltages and currents of lamps that can be used for diagnosing baseline problems Refer to the Service Manual for details 1200 Series VWD Service Manual Troubleshooting and Diagnostics 7 Status Indicators Two status indicators are located on the front of the detector The lower left indicates the power supply status the upper right indicates the detector status Status indicator d green yellow red Bum i M Line power switch s D 7 r with green light Figure 51 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 1200 Series VWD Service Manual 103 7 104 Troubleshooting and Diagnostics Detect
70. ag black cover long life deuterium lamp long life deuterium lamp black cover standard deuterium lamp G1314D E VWD preferred G1314D E VWD G1315A B C D G1365A B C D G1314A B C see note below RFID tag info used in G1315C D and G1365C D only G1315A B C D G1365A B C D G1314A B C see note below G1315A B C D G1365A B C D G1314A B C see note below G1315A B C D G1365A B C D G1314A B C see note below 1200 Series VWD Service Manual 143 10 Test Functions for Service 144 These lamps can be operated in the Agilent 1200 series variable wavelength detector G1314A B C D E as well For details about changing the configuration and information on the specifications refer to the Exchanging a Lamp on page 165 Optimization Information To extend the useful life of deuterium lamps in the 1200 Series VWD we recommend to turn lamps off over night or when not used for gt 4 hours Wander Drift Problems Due to Temperature Changes The most frequent cause of ambient temperature fluctuations are unstable laboratory air conditioning systems Other causes include direct sunshine or drafts from open doors and windows These temperature changes cause baseline wander which can make reproducible integration of trace level peaks difficult or impossible Warm up of Detector Give the optical unit enough time to warm up and stabilize gt 60 minutes The detector is temperature controlled After turn
71. airs Overview of Main Assemblies Table 34 Main Assemblies Item Description Part Number 1 Main board VWM for G1314D VWD G1314 65070 1 Main board VWM for G1314D VWD exchange part G1314 69070 1 Main board VWM for G1314E VWD SL Plus 61314 65080 1 Main board VWM for G1314E VWD SL Plus exchange part 61314 69080 Cable CAN assembly 5181 1516 Hexagonal nut for RS 232C connector 1251 7788 Nut for analog connector 2940 0256 Washer for analog connector 2190 0699 2 Power supply assembly additional power and status light parts 0950 2528 see Power and Status Light Pipes on page 258 3 Optical unit additional optical unit parts see Optical Unit on G1314 60062 page 250 3 Optical unit exchange part 61314 69062 4 Fan assembly 61314 65004 5 Deuterium lamp with RFID tag G1314 60101 6 Heater Assembly G1314 60113 7 Standard Flow cell see Standard Flow Cell 10 mm 14 ul on 61314 60186 page 236 61314 60187 Micro Flow Cell see Micro Flow Cell 3 mm 2 ul on page 238 61314 60183 Semi Micro Flow Cell see Semi micro Flow Cell 6 mm 5 61314 60182 ul on page 240 High Pressure Flow Cell see High Pressure Flow Cell 10 mm 14 ul on page 242 Leak handling parts see Leak Parts on page 256 9 Leak sensor assembly 5061 3356 248 1200 Series VWD Service Manual Parts for Repairs 15 Table 34 Main Assemblies Item Description Part Number 10 Heater Interface and Cable see Heater and Fan on page 252
72. amp interface 6 Liftthe optical unit out of the instrument connector 1200 Series VWD Service Manual 203 13 Repair Installing the Optical Unit Installing the Optical Unit When To continue the installation Tools required Screwdriver POZI 1 PT3 Wrench 1 4 inch Preparations The power supply the temperature sensor the fan and the main board are already installed NOTE For ESD information refer to Cautions and Warnings on page 180 1 Observe that the leak sensor cable and the lamp cable are fitted in their foam channels N 2 Observe that all bumpers in their location in the bottom foam mm n HHHHHHERHEHHHEEHHHEHES 204 1200 Series VWD Service Manual 13 Repair Installing the Optical Unit 3 Place the optical unit into the bottom foam 4 Reconnect all cables to the main board 5 Slide the flat cable into the connector and flip the black holder back For details see next figure xe 7 6 Details of connector cable 1200 Series VWD Service Manual 205 13 Repair Installing the Optical Unit 7 Replace the Heater Assembly and the plate that secures 8 Next Steps the optical unit Replace the Metal Cover the Closure and the Front Panel see Installing the Foam and the Top Cover on page 229 Replace the detector in the system 206 1200 Series VWD Service Manual R
73. anual This chapter provides addition information on safety legal and web 1200 Series VWD Service Manual 18 Appendix General Safety Information 300 Radio Interference 303 Sound Emission 304 UV Radiation 305 Solvent Information 306 Declaration of Conformity for HOX2 Filter 308 Agilent Technologies on Internet 309 RE Agilent Technologies 299 18 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure the proper usage of the equipment The protection provided by the equipment may be impaired The operator of this instrument is advised to use the equipment in a manner as specified in this manual General This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation 300 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all prote
74. ata Rate Hz 0 0012 0 03 160 20 0012 0 03 160 20 0025 0 06 160 20 005 0 12 80 20 0125 0 25 40 20 025 0 5 20 20 05 1 0 10 20 10 2 0 5 20 20 4 0 2 5 gt 0 40 8 0 1 25 92 1200 Series VWD Service Manual 1200 Series VWD Service Manual 6 How to optimize the detector Optimizing the Detector Performance 94 Match the Flow Cell to the Column 95 Set the Detector Parameters 99 This chapter gives hints on how to select the detector parameters and the flow cell RE Agilent Technologies 93 6 How to optimize the detector Optimizing the Detector Performance The detector has a variety of parameters that can be used to optimize performance The information below will guide you on how to get the best detector performance Follow these rules as a start for new applications It gives a rule of thumb for optimizing the detector parameters 94 1200 Series VWD Service Manual How to optimize the detector 6 Match the Flow Cell to the Column The tables below recommend the flow cell that matches the column used If more than one selection is appropriate use the larger flow cell to get the best detection limit Use the smaller flow cell for best peak resolution Standard HPLC Applications Column length Typical peak width Recommended flow cell lt 5 cm 0 025 min Micro flow cell 10 cm Semimicro flow cell gu cm Ex 1 min gt er NI mr ypical flow rate ELI M 05 0 2 ml min o 2 0 4 ml min remi 4 0 8 ml min lt a 2 m
75. ately Table9 Using Default Parameters IP address 192 168 254 11 Subnet Mask 255 255 255 0 Default Gateway not specified Since the default IP address is a so called local address it will not be routed by any network device Thus the PC and the module must reside in the same subnet 1200 Series VWD Service Manual 51 4 52 LAN Configuration The user may open a Telnet session using the default IP address and change the parameters stored in the non volatile memory of the module He may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the module is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network the user may simply keep the default parameters to establish the TCP IP connection In the Using Default mode the parameters stored in the memory of the module are not cleared automatically If not changed by the user they are still available when switching back to the mode Using Stored 1200 Series VWD Service Manual LAN Configuration 4 Link configuration selection The LAN interface supports 10 or 100 Mbps operation in full or half duplex modes In most cases full duplex is supported when the connecting network device such as a network switch or hub supports IEEE 802 3u auto negotiation specifications When connecting to network d
76. be performed with a clean and bubble free flow cell and with a new deuterium lamp or one in good condition In case of the following problem symptoms call your local Agilent Technologies service representative for help low intensity readings after replacement of lamp and or cleaning of the flow cell and or source lens assembly calibration failures zero order holmium oxide lines or 656 nm emission lines excessive noise and or drift grating drive failures filter drive failures and no intensity readings on one or both the reference and or sample side Special Notes There are no repairs within the optical unit possible since this requires special knowledge and special tools affecting the alignment of optical components In case of problems the complete optical unit must be replaced Incase of problems that can be related to the optical unit e g intensity or calibration problems the optical unit cover may be opened for visual check of contaminated or broken loose parts like mirrors or grating 1200 Series VWD Service Manual 199 13 Repair If optical components like mirrors or grating show signs of contaminations this will reduce the light throughput drastically In this case read Contaminated Mirrors and Grating on page 200 first Contaminated Mirrors and Grating Under some specific environmental conditions it might happen that the efficiency of the mirrors and the grating is decreasing faster than
77. called Bootstrap Protocol by manually setting the parameters using Telnet by manually setting the parameters using the Instant Pilot G4208A or by manually setting the parameters using the Handheld Controller G1323B if supported The LAN interface differentiates between several initialization modes The initialization mode short form init mode defines how to determine the active TCP IP parameters after power on The parameters may be derived from a Bootp cycle non volatile memory or initialized with known default values The initialization mode is selected by the configuration switch see Table 10 on page 53 1200 Series VWD Service Manual 47 4 LAN Configuration Configuration Switches The configuration switch can be accessed at the rear of the module see figure below Figure 10 Location of Configuration Switch The module is shipped with all switches set to OFF as shown above To perform any LAN configuration SW1 and SW2 must be set to OFF Table 7 Factory Default Settings Initialization Init Mode Bootp all switches down For details see Figure 11 on page 49 Link Configuration speed and duplex mode determined by auto negotiation for details see Link configuration selection on page 53 48 1200 Series VWD Service Manual LAN Configuration 4 Initialization mode selection The foll
78. cations on page 31 below the table Under specified condtions See Specifications on page 31 below the table Under specified condtions See Specifications on page 31 below the table Self calibration with deuterium lines verification with holmium oxide filter G1314D VWD G1314E VWD SL PLus G1314E VWD SL PLus Not supported with ChemStation B 03 02 SR1 28 1200 Series VWD Service Manual Site Requirements and Specifications 2 Table4 Performance Specifications G1314D VWD and G1314E VWD SL Plus Type Specification Comments Flow cells Standard 14 pl volume 10 mm All flow cells have RFID tags Electronic Temperature Control ETC Control and data evaluation Time programmable Spectral tools Analog outputs Communications for unambitious identification Can be repaired on component level cell path length and 40 bar 588 psi pressure maximum High pressure 14 pl volume 10 mm cell path length and 400 bar 5880 psi pressure maximum Micro 2 ul volume 3 mm cell path length and 120 bar 1741 psi pressure maximum Semi micro 5 ul volume 6 mm cell path length and 40 bar 588 psi pressure maximum For improved baseline stability in instable environment Control and data evaluation Control only Agilent ChemStation for LC B 03 02 SR1 or above Instant Pilot 64208A with firmware B 02 07 or above Wavelength Reference and Sample scan balance steps lamp on off Stop flow wavelen
79. ce PC and Firmware Update Tool with local files on the hard disk or Instant Pilot G4208A with files from a USB Flash Disk or handheld control module G1323A B with files from a PC card The file naming conventions are PPPP RVVV XX dlb where PPPP is the product number for example 1315AB for the G1315A B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 102 is revision 1 02 XX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Some 1200 series modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some 1200 series SL modules like the G1312B G1314C G1316B can be downgraded to lower versions by converting the module into a lower version for example a G1312B SL pump is converted to a G1312A and looses the features of the G1312B All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http
80. ce it on the working bench Remove the Front Panel the Closure and the Metal Cover see Removing the Top Cover and Top Foam on page 182 1 The status light pipe is clipped into the top cover 2 Next Steps Replace the Metal Cover the Closure and the Front Panel see Installing the Foam and the Top Cover on page 229 Replace the detector in the system 1200 Series VWD Service Manual 209 13 Repair Exchanging the Main Board and Power Supply Prerequisites When Tools required Parts required Preparations 210 If the detector main board is defective Screwdriver POZI 1 PT3 Hexagonal wrenches 5 mm and 14 mm Part number Description 1 G1314 69080 Detector main board VWM G1314E SL Plus exchange assembly 1 G1314 65080 Detector main board VWM G1314E SL Plus 1 G1314 69070 Detector main board VWM G1314D exchange assembly 1 G1314 65070 Detector main board VWM G1314D 1 0950 2528 Main Power Supply Turn OFF the detector Disconnect the power cable Disconnect capillaries Remove detector from the system and place it on the working bench Remove the Front Panel the Closure the Metal Cover and the Top Foam see Removing the Top Cover and Top Foam on page 182 Remove the Heater Assembly see Replacing the Heater Assembly on page 195 Follow the detailed instructions 1 Removal of Main Board 2 Replacing Main Power Supply Power Light Pipe 3 Installation of Main Board 4 Next
81. cell to close the air flow see Chapter Removing the Top Cover and Top Foam on page 182 Step 6 and 7 186 1200 Series VWD Service Manual Repair 13 Replacing the Temperature Sensor 1 Unplug the lamp connector from the main board 2 Disconnect the temperature sensor from the main board TEMP SENS 2 4 Carefully lift the board from the holder using a small flat screw driver 1200 Series VWD Service Manual 187 13 Repair Replacing the Temperature Sensor 5 Add the new Temperature Sensor to the holder Assure 6 Insert the Temperrature Sensor into the foam that the board fits flat on the holder 7 Route the sensor cable in its channel in the foam towards 8 Reconnect the Temperature Sensor Cable to the the main board should come up between the connector for grating and filter connector TEMP SENS 2 on the main board 188 1200 Series VWD Service Manual Repair 13 Next Steps 9 Replace the Top Foam the Metal Cover the Closure and the Front Panel see Installing the Foam and the Top Cover on page 229 10 Replace the detector in the system 11 Replace and reconnect the fan see Exchanging the Fan on page 190 step 2 12 Replace the optical unit see Installing the Optical Unit on page 204 13 Replace the Top foam see Installing the Foam and the Top Cover on page 229 14 Replace the heater assembly see Replacing the H
82. cht Agilent Technologies Germany last modification 09 11 2008 This macro can be used to open add additional signals to the Agilent ChemStation for measurements in unstable environment problems with drift wander noise From the ChemStatiion command line type macro tempmon mac go Name TempMon I listmessages on 400 400 Popup window to list all messages during execution print sendmodule nvwd RAWD SIGSTOR ADD 0 A Sig A print sendmodule nvwd RAWD SIGSTOR ADD 1 B Sig B print sendmodule nvwd RAWD SIGSTOR ADD 2 Sig C print sendmodule nvwd RAWD SIGSTOR ADD 0 1 2 3 D Sample intensity print sendmodule nvwd RAWD SIGSTOR ADD 4 5 6 7 E Reference intensity F Board Temperature G Optical unit Temp Sensor for TCTL H UV Lamp voltage print sendmodule nvwd RAWD SIGSTOR ADD print sendmodule nvwd RAWD SIGSTOR ADD print sendmodule nvwd RAWD SIGSTOR ADD print sendmodule nvwd RAWD SIGSTOR ADD 14 O AUX Sig 0 print sendmodule nvwd RAWD SIGSTOR ADD 15 P AUX Sig 1 print sendmodule nvwd RAWD SIGSTOR ADD 16 Q AUX Sig 2 print sendmodule nvwd RAWD CSIGMON ADD 3 print sendmodule nvwd RAWD CSIGMON ADD 4 print sendmodule nvwd RAWD CSIGMON ADD 5 6 7 D Sample intensity E Reference intensity F Board Temperature print sendmodule nvwd RAWD CSIGMON ADD G Optical unit Temp Sensor for TCTL print sendmodule nvwd RAWD CSIGMON ADD H UV Lamp voltage p
83. configuration 36 standards 27 status indicators 102 103 stepper motor 16 stop flow condition 84 storing the settings permanently 58 T TCP IP parameter configuration 47 telnet configuration 59 Temp Sensor replacement 186 temperature control configuration 83 temperature control 29 282 test functions 102 123 133 tests available tests vs interface 105 DAC digital to analog 134 grating motor 136 holmium oxide 130 intensity of deuterium lamp 124 photocurrent readings 126 test chromatogram 140 wavelength calibration 128 troubleshooting available tests vs interface 105 diagnostic signals 102 error messages 108 error messages 102 overview 102 status indicators 102 103 1200 Series VWD Service Manual Index test functions 102 123 133 type recover with ChemStation 227 with Control Module 228 with Instant Pilot 227 type and firmware recover with LMD 226 type recover with LMD 226 type changing 217 U unpacking 34 USB 283 using analog output settings 89 control settings 82 detector parameters 76 drift 74 EMF 21 load method 75 online plot 78 online spectra 84 peakwidth settings 91 preparing the HPLC system 72 priming and purging the system 68 requirements and conditions 70 running the sample 81 sample info 80 scanning 85 setting up an analysis 68 setup of detector 76 signal plot 78 special setpoints 90 special settings 82 stable baseline 74 stop flow condition 84 the
84. connection 1200 Series VWD Service Manual 261 16 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent 1100 1200 Series modules The other end depends on the instrument to which connection is being made Agilent 1100 1200 to 3390 2 3 Integrators Connector01040 60101 Pin 3390 2 3 Pin Agilent Signal Name 1100 1200 1 Shield Ground 2 Not connected 7 S 3 Center Signal 6 2 RD 4 Connected to pin 6 aA 5 Shield Analog BRN 6 Connected to pin 4 7 Key 8 Not connected 262 1200 Series VWD Service Manual Identifying Cables 16 Agilent 1100 1200 to 3394 6 Integrators Connector35900 60750 Pin 3394 6 Pin Agilent Signal Name 1100 1200 1 Not connected 2 Shield Analog 3 Center Analog cera Agilent 1100 1200 to BNC Connector Connector8120 1840 Pin BNC Pin Agilent Signal Name 1100 1200 3 ZA FTN Shield Shield Analog MC EO Center Center Analog 1200 Series VWD Service Manual 263 16 Identifying Cables Agilent 1100 1200 to General Purpose Connector01046 60105 Pin 3394 6 Pin Agilent Signal Name 1100 1200 1 Not connected 2 Black Analog p 3 Red Analog t 264 1200 Series VWD Service Manual Remote Cables O O oleas elo Identifying Cables 16 One end of these cable
85. correct module type e g G1315B Save and close the file Insert the USB Flash Drive into the Instant Pilot From the Welcome screen enter the Service Mode 7268312 or SERVICE Select the button G1319B wrong module and select XXXXX Command from USB Type Change Press the button Send This will give as reply RA 0 TYPE G1315B After a power cycle the module should show up with the correct product number type in the user interface 1200 Series VWD Service Manual 227 13 Repair Recover with Control Module The example uses G1315B as correct type 1 Select Tests Generic 2 Press keys m m m dot m This opens hidden functions 3 Select Command 4 In the instruction line enter the command TYPE G1315B This will give as reply RA 0000 TYPE G1315B 5 After a power cycle the module should show up with the correct product number type in the user interface 228 1200 Series VWD Service Manual Repair 13 Installing the Foam and the Top Cover Installing the Foam and the Top Cover When When all repairs have been completed Tools required Screwdriver POZI 1 PT3 Preparations The detector is open and other procedures have been carried out All previous repairs have been completed NOTE First units have pre released foam parts Therefore a single piece of foam is located between optical unit and front sheet metal right of the flow cell to close the air flow 1 Carefully fit the top foam into the de
86. ctive earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious 1200 Series VWD Service Manual Appendix 18 personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite
87. ctromechanically actuated During wavelength calibrations it moves into the light path Cutoff Filter plus Holmium Oxide Filter Shutter Cutoff Filter Figure 3 Filter Assemby The filter assembly has two filters installed and is processor controlled OPEN nothing in light path at A lt 370 nm CUTOFF cut off filter in light path at A gt 370 nm HOLMIUM holmium oxide filter for wavelength check SHUTTER for measurement of dark current of photo diodes A photo sensor determines the correct position Mirror Assemblies M1 and M2 The instrument contains two spherical mirrors M1 and M2 The beam adjustable is vertically and horizontally Both mirrors are identical 1200 Series VWD Service Manual 15 1 Introduction to the Variable Wavelength Detector Grating Assembly The grating separates the light beam into all its component wavelengths and reflects the light onto mirror 2 The stepper motor reference position is determined by a plate fitted onto the motor shaft interrupting the beam of a photo sensor The wavelength calibration of the grating is done at the zero order light position and at 656 nm which is the emission line of the deuterium lamp Beam Splitter Assembly The beam splitter splits the light beam One part goes directly to the sample diode The other part of the light beam goes to the reference diode Photo Diodes Assemblies Two photo diode assemblies are installed in the optical unit The
88. cuvette holder 173 the detector 6 7 1200 Series VWD Service Manual turnon 73 typical chromatogram 71 warmup 74 UV radiation 305 Ww wander diagnostic 144 warm up of detector 74 144 warnings and cautions 159 180 wavelength accuracy 28 calibration 128 range 190 600 nm 28 recalibration 102 weight 27 315 www agilent com In This Book This manual contains The manual describes the following introcduction and specifications installation using and optimizing troubleshooting and diagnose maintenance and repair parts identification hardware information safety and related information O Agilent Technologies 2008 Printed in Germany 09 08 G1314 90130 d Agilent Technologies
89. der Lamp or Flow Cell When If the RFID tag reader is defective Tools required Screwdriver POZI 1 PT3 Preparations Turn OFF the detector Disconnect the power cable Disconnect capillaries Remove detector from the system and place it on the working bench Remove the Front Panel the Closure the Metal Cover and the Top Foam see Removing the Top Cover and Top Foam on page 182 Remove the Heater Assembly see Replacing the Heater Assembly on page 195 Remove the optical unit see Removing the Optical Unit on page 201 Disconnect and remove the fan assembly 1 Disconnect the sensor cable s from the main board 2 If you want to replace the RFID Reader Cell continue with step 5 without performing steps 3 and 4 192 1200 Series VWD Service Manual Repair 13 Replacing the RFID Tag Reader Lamp or Flow Cell 3 Ifyou want to replace the RFID Reader Lamp unscrew the 4 Remove the optical cover from the housing four screws the fix the cover of the optical Don t lose the screws o rings and spring washers 5 Carefully unscrew the assembly replace it by the new 6 Replace the optical cover and fix the four screws The assembly and fix it with the two screw order of the screw parts is shown below RFID Reader Lamp OLIO RFID Reader Cell 1200 Series VWD Service Manual 193 13 Repair Replacing the RFID Tag Reader Lamp or Flow Cell 7 Reconnect the connectors to
90. e terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi
91. e Glass Filter Standards and Technology NIST to be applied as certified wavelength standard According to the publication of NIST in J Res Natl Inst Stand Technol 112 303 306 2007 the holmium oxide glass filters are inherently stable with respect to the wavelength scale and need no recertification The expanded uncertainty of the certified wavelength values is 0 2 nm Agilent Technologies guarantees as required by NIST that the material of the filters is holmium oxide glass representing the inherently existent holmium oxide absorption bands Test wavelengths Product Series Measured Wavelength Optical Number Wavelength Accuracy Bandwidth 79883A 361 0 nm 1 nm 2 nm 79854A 418 9 nm G1306A 453 7 nm G1315A 1100 536 7 nm G1315B C 1100 1200 G1600 79853C 1050 360 8nm 2 nm 6nm 418 5nm 536 4nm G1314A B C 1100 1200 360 8nm 1 nm 6 nm G1314D E 418 5nm G4286 90A 1120 536 4nm The variation in Measured Wavelength depends on the different Optical Bandwidth March 04 2008 Date secas frie Po E R amp D Manager Quality Manager P N 89550 90501 Revision G Effective by Mar 04 2008 1200 Series VWD Service Manual Agilent Technologies Appendix 18 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis
92. e Manual 73 5 74 Using the Detector 5 Purge the pump For more information Priming and Purging the System on page 68 6 Allow the detector to warm up of at least 60 minutes to provide a stable baseline example Figure 33 on page 74 For reproducible chromatography the detector and lamp should be on for at least one hour Otherwise the detector baseline may still drift depending on the environment Figure 33 heater control is OFF heater control is ON 20 40 50 Stabilization of baseline after turn on of detector 7 For the isocratic pump fill the solvent bottle with the mixture of HPLC grade bi distilled water 30 and acetonitrile 70 For binary and quaternary pumps you can use separate bottles 1200 Series VWD Service Manual mij Using the Detector Setting up an Analysis 8 Click on the Load Method button and select DEF_LC M and press OK Alternative double click on the method in the method window The default LC method parameters are transferred into the 1200 modules Name File RunControl Instrument Method Sequence View Abort Help Load Method System 2 2 x DEF LCM BATCH M E M DEMOCAL2 M DGALSTST M DGCALAS M DGCALOG1 M DGCALOQ2 M c chem32 2 methods Sa Cancel c E chem32 G2 E gt methods n Drives c SYSTEM z Network 5 E Methods GA bd per Lc Sequences Lgs led oer
93. e for LAN for specifics LAN modes switches 3 8 must be set as required for boot test modes switches 1 2 must be UP plus required mode The modules have a new main board platform that differs from the other modules Therefore the use of the 8 bit configuration switch has been changed Refer to the module specific reference manual in case you need detailed information on an other 1200 module Figure 90 Location of Configuration Switch The 8 bit configuration switch is located at the rear of the module Switch settings provide configuration parameters for LAN serial communication protocol and instrument specific initialization procedures To perform any LAN configuration SW1 and SW2 must be set to OFF For details on the LAN settings configuration refer to chapter LAN Configuration 1200 Series VWD Service Manual 293 17 Hardware Information Table 44 8 bit Configuration Switch Function SW 3 Link Configuratio Mode Selection Auto negotiation 10 MBit half duplex 10 MBit full duplex 100 MBit half duplex 100 MBit full duplex Bootp Bootp amp Store Using Stored Using Default Watchdog Boot Resident System x Stop Early To Debug Disable Watchdog Revert to Default Data Coldstart Legend 0 switch down
94. eady condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause Suggested actions 1 Not ready condition in one of the Ensure the instrument showing the not ready instruments connected to the remote line condition is installed correctly and is set up correctly for analysis 2 Defective remote cable Exchange the remote cable 3 Defective components in the instrument Check the instrument for defects refer to the showing the not ready condition instrument s reference documentation Synchronization Lost During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly 2 Defective CAN cable Ensure all CAN cables are installed correctly 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not rec
95. eater Assembly on page 195 1200 Series VWD Service Manual 189 13 Repair Exchanging the Fan When If the fan is defective or noisy Tools required Screwdriver POZI 1 PT3 Parts required Part number Description 1 3160 1016 Fan Preparations Turn OFF the detector Disconnect the power cable Disconnect capillaries Remove detector from the system and place it on the working bench Remove the Front Panel the Closure the Metal Cover and the Top Foam see Removing the Top Cover and Top Foam on page 182 Remove the Heater Assembly see Replacing the Heater Assembly on page 195 WARNING Injury by sharp metal edges gt Be careful when touching the RFI sheet metal at the rear of the fan There are sharp edges 190 1200 Series VWD Service Manual Repair 13 Exchanging the Fan 1 Disconnect the fan from the main board and remove the 2 Fit the fan assembly into its location Replace and fan assembly reconnect the fan assembly to the main board 3 Next Steps NOTE Replace the Top Foam the Metal Cover the Closure and the Front Panel see Installing the Foam and the The fan must be installed in the correct orientation to ensure Top Cover on page 229 optimum cooling and operation of the detector The direction Replace the detector in the system of air flow is from the front towards the rear 1200 Series VWD Service Manual 191 13 Repair Replacing the RFID Tag Rea
96. ed is different than the original module serial number you will be given the opportunity to edit the configure 1200 access screen during the restart of the Agilent ChemStation 4 After restart the serial number type you have just entered can be seen under the Instrument menu of the main user interface screen 220 1200 Series VWD Service Manual Repair 13 Using the Instant Pilot G4208A Connect the Instant Pilot to the module Turn ON the module 2 On the Instant Pilot s Welcome screen press More then select Maintenance Using the up down arrows select the module where you have to change the product number or serial number 3 Press PN SN This will display a screen where you can enter the product number and or serial number 4 Make your changes using the information from the product label of your module Some Agilent 1100 1200 series modules require the correct main board version to match the type for example the SL modules 5 Press OK to highlight the complete command 6 Press Done to transfer the information into the main board s memory Press Cancel to quit the process 7 Power cycle the module The Maintenance screen should display the correct serial number for this module 8 If an other User Interface is also connected restart the User Interface as well 1200 Series VWD Service Manual 221 13 Repair 222 Using the Control Module G1323B Connect the control module to the module Turn ON the module On
97. ength maxima of the built in holmium oxide filter The test displays the difference between the expected and measured maxima The figure below shows a holmium test spectrum The Holmium Oxide Test is available in Agilent Diagnostic Software preferred tool Agilent Instant Pilot G4208A via More Diagnosis VWD Holmium Spectrum Test The test uses the following holmium maxima 360 8 nm 418 5 nm e 536 4 nm See also Holmium Declaration UV Detectors Declaration of Conformity for HOX2 Filter on page 308 When to do the Test after recalibration as part of the Operational Qualification Performance Verification procedure or after flow cell maintenance or repair Interpreting the Results The test is passed successfully when all three wavelengths are within 1 nm of the expected value This indicates the detector is calibrated correctly 1200 Series VWD Service Manual Test Functions for User 9 Holmium Oxide Test Holmium Oxide Test with Instant Pilot Diagnosis Holmium VWD SL 360 380 400 420 440 460 480 500 520 nm Action Result Wait until lamp s ON Done A Wait until UV lamp has warmed up Done Perform holmium test Done WL 1 358 8nm 362 8nm measured 360 8 Passed WL 2 416 5nm 420 5nm measured 418 3 Passed WL 3 534 4nm 538 4nm measured 536 7 Passed Finished Passed Y Used to verify wavelength accuracy T 10 12
98. ent touch one of the metal housing panels at the front of the instrument 1 Press the release buttons and remove the front coverto 2 Unscrew the heater assembly and remove it have access to the front area 166 1200 Series VWD Service Manual Maintenance 12 Exchanging a Lamp 3 Unscrew disconnect and remove the lamp Insert fix and 4 Replace the heater assembly reconnect the lamp Next Steps 5 Replace the front cover 6 Reset the lamp counter as described in the User Interface documentation required for non RFID tag lamps only 7 Turn the lamp ON 8 Give the lamp more than 10 minutes to warm up 9 Perform Wavelength Verification Calibration on page 128 to check the correct positioning of the lamp NOTE If the detector was turned off during the replacement then the detector requires a warm up time of 60 minutes No measurements should be performed during this time 1200 Series VWD Service Manual 167 12 Maintenance Exchanging a Flow Cell When If application needs a different type of flow cell or the flow cell needs repair Tools required Two 1 4 inch wrenches for capillary connections Parts required Description For flow cells see Overview of Maintenance Parts on page 234 Preparations Turn the lamp OFF 1 Press the release buttons and remove the front coverto 2 Disconnect the inlet and outlet capillaries have access to the flow cell area
99. epair 13 Exchanging the Leak Sensor When When leak sensor is defective Tools required Screwdriver POZI 1 PT3 Preparations Turn OFF the detector Disconnect the power cable Disconnect capillaries Remove detector from the system and place it on the working bench Remove the Front Panel the Closure the Metal Cover and the Top Foam see Removing the Top Cover and Top Foam on page 182 Remove the optical unit see Removing the Optical Unit on page 201 1 Disconnect the leak sensor assembly on the main board 2 Remove the leak sensor assembly from the leak panel B 1200 Series VWD Service Manual 207 13 Repair Exchanging the Leak Sensor 3 Replace the Leak Sensor 4 Route the Leak Sensor Cable back through the opening and fit it into the bottom foam channel 5 Install the Optical Unit see Installing the Optical Next Steps Unit on page 204 6 Replace the Top Foam the Metal Cover the Closure and the Front Panel see Installing the Foam and the Top Cover on page 229 7 Replace the detector in the system 208 1200 Series VWD Service Manual Repair 13 Replacing Status Light Pipe When When part is broken Tools required Screwdriver POZI 1 PT3 Parts required Part number Description 1 5041 8384 Status light pipe Preparations Turn OFF the detector Disconnect the power cable Disconnect capillaries Remove detector from the system and pla
100. epairing the Flow Cells 7 Perform Wavelength Verification Calibration on page 128 to check the correct positioning of the flow cell 8 Replace the front cover 172 1200 Series VWD Service Manual Maintenance 12 Using the Cuvette Holder This cuvette holder can be placed instead of a flow cell in the variable wavelength detector Standard cuvettes with standards in it for example National Institute of Standards amp Technology NIST holmium oxide solution standard can be fixed in it This can be used for wavelength verifications Parts required Part number Description 1 61314 60200 Cuvette Holder 1 Cuvette with the standard e g NIST certified holmium oxide sample 1200 Series VWD Service Manual 173 12 Maintenance 1 Locate the cuvette holder on the desk 2 Unscrew the bracket ae 3 Insert the cuvette with the sample into the holder The 4 Replace the bracket and fix the cuvette clear side of the cuvette must be visible Light path Clear side Next Steps 5 Install the cuvette holder in the instrument 6 Perform your Wavelength Verification Calibration Wavelength Verification Calibration on page 128 to check the correct position of the cuvette holder 174 1200 Series VWD Service Manual Maintenance 12 Correcting Leaks Correcting Leaks When If a leakage has occurred in the flow cell area or at the capillary connections Tools required Tissue
101. eps of 1 96 Attenuation Limits 0 98 to 4000 mAU at discrete values for either 100 mV or 1 V full scale gt additional signals can be stored with the normal signal for diagnostics gt autobalance to zero absorbance on the analog output plus offset at begin and or end of run see Special Setpoints on page 90 Figure 36 Detector Settings default 1200 Series VWD Service Manual TI 5 Using the Detector 11 Pump the water acetonitrile 30 70 mobile phase through the column for 10 minutes for equilibration 12 Click the button and select Change to open the Signal Plot information Select the Pump Pressure and the VWD A Signal 254 as signals Change the Y range for the VWD to 1 mAU and the offset to 20 6 and the pressure offset to 50 The X axis range should be 15 minutes Press OK to exit this screen Method and Run Control SN caren MI orc o forse EM sO o E 0 bar og Ne DEFAULT D 3 Edit Signal Plot Loy Available Signals Selected Signals 50 ja 40 uaternary Pump Flow Quaternary Pump Pressure 30 uaternary Pump A zm VWD A Absorbance at 254 nm 20 uaternary Pump B EAN 40 uaternary Pump 9C z uaternary Pump 9D E 1 1 GB free olumn Thermostat Temperature l olumn Thermostat Temperature ri WWD B Signal w o Reference at 254 v Window x axis 15 d min draw zero line VWD A Absorbance at 254 nm
102. er supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply The security lever at the power input socket prevents the module cover from being taken off when line power is still connected Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1200 Series VWD Service Manual 17 1 Introduction to the Variable Wavelength Detector Electrical Connections REMOTE cc er DC DC dE AC gt Do gt oeo EE CAN CAN Ww EI 7 ojo SHE Eo wene A c9 COMPACT FLASH G1314E VWD SL G1314E VWD SL Serial No Serial No X Agilent Technologies G soe X LR63703 N10149 ICES NMB 001 mummu Agilent Technolagies 76337 Waldbronn Made in Germany Figure 4 Rear View of G1314D E VWD 18 1200 Series VWD Service Manual Relay contacts Vial number output
103. er Cable 61314 81010 5 Holder Temp Sensor G1314 44016 6 Temp Sensor Board G1314 65802 7 Temp Sensor Cable G4280 81620 252 1200 Series VWD Service Manual Parts for Repairs 15 Heater and Fan Figure 78 Heater and Fan 1200 Series VWD Service Manual 253 15 Parts for Repairs Sheet Metal Kit Table 37 Sheet Metal Kit Parts Item Description Part Number Sheet metal kit includes items 1 2 3 and 4 1x Top cover Case Plate FP wo N Screws M3 for cover and plate 61314 68000 5022 2112 Mm t MW 7 Figure 79 Sheet Metal Kit 254 1200 Series VWD Service Manual Plastic Parts Table 38 Plastics Parts Parts for Repairs 15 Item Description Part Number 1 Front cover 5065 9982 2 Plastics includes base sides and top 5065 9985 3 Name plate Agilent 1200 Series 5042 8901 For correct assembling of the top and sides see Assembling the Main Cover on page 231 Figure 80 Plastic Parts 1200 Series VWD Service Manual 255 15 Parts for Repairs Leak Parts 256 Table 39 Leak Parts Item Description Part Number 1 Leak sensor assembly 5061 3356 2 Leak pan 5042 8908 3 Leak funnel 5041 8388 4 Leak funnel holder 5041 8389 5 Clip 5041 8387 6 Corrugated tubing 120 mm lg re order 5 m 5062 2463 7 Corrugated tubing 1200 mm lg re order b m 5062 2463 Figure 81 Leak Parts 1200 Series VWD Service Manual Parts for Repairs 15 Foam Parts Table 40 F
104. er into the light path If the filter is functioning correctly a decrease in lamp intensity is seen If the expected intensity decrease is not detected the error message is generated Probable cause Suggested actions 1 Filter motor defective Run the filter motor test to determine if the motor is defective 2 Defective or missing filter Exchange the filter assembly Filter Missing The filter motor is not detected Probable cause Suggested actions 1 Filter motor is not connected Re connect the filter motor on the main board 2 Cable connector defective Replace the optical unit or main board 118 1200 Series VWD Service Manual Grating Missing The grating motor is not detected Probable cause 1 Grating motor is not connected 2 Cable connector defective ADC Hardware Error A D Converter hardware is defective Probable cause 1 A D Converter hardware is defective 1200 Series VWD Service Manual Error Information Suggested actions Re connect the filter motor on the main board Replace the optical unit or main board Suggested actions Replace the optical unit 119 120 Error Information Illegal Value From Temperature Sensor This temperature sensor delivered a value outside the allowed range The parameter of this event equals the measured temperature in 1 100 centigrade As a result the temperature control is switched off Probable cause 1 The sensor is defect 2 Detector is exposed
105. ermine the rate of temperature change Look for trends which prevail for 5 minutes or longer Turbulences around one minute or less can be ignored Figure 59 Diagnostic signals added to normal signal 1200 Series VWD Service Manual 147 10 Test Functions for Service Additional Signals The detector provides additional standard signals that can be used for troubleshooting These are Signal without Reference Reference only These can be set via the Agilent ChemStation xi r Signal Time r Analog Output Wavelength Stoptime Zero Offset 254 nm 60 00 min x Peakwidth Hesponsetime Posttime Attenuation O1min 2s zj Off 4 min 1000 v mAU r Timetable gt Store additionally 1 Line Time Wavelength Balance Scan met IV Signal w o Reference Append vi 3 Cut r Autobalance Cony Iv Pren E E Eee Postrun f Table C Graphic F Special Setpoints TT te Figure 60 Additional Signals on ChemStation Settings The figure below shows an example with a stable environment The signals Sample and Reference are shown in addition to the standard signal 148 1200 Series VWD Service Manual Test Functions for Service 10 Diagnostic Signals Figure 61 Standard top Sample mid and Reference bottom during warm up 1200 Series VWD Service Manual 149 10 Test Functions for Service Adding an External Temperature Sensor for Diagnostic Purpose There a
106. ervice Manual Contents Agilent Technologies on Internet 309 1200 Series VWD Service Manual Contents 8 1200 Series VWD Service Manual 1200 Series VWD Service Manual 1 Introduction to the Variable Wavelength Detector Introduction to the Detector 10 Optical System Overview 12 Electrical Connections 17 Instrument Layout 20 Early Maintenance Feedback EMF 21 This chapter gives an introduction to the detector instrument overview and internal connectors OE Agilent Technologies 1 Introduction to the Variable Wavelength Detector Introduction to the Detector 10 The Agilent 1200 Series variable wavelength detector is designed for highest optical performance GLP compliance and easy maintenance with deuterium lamp for highest intensity and lowest detection limit over a wavelength range of 190 to 600 nm optional flow cell cartridges standard 10 mm 14 ul high pressure 10 mm 14 ul micro 3 mm 2 ul semi micro 6 mm 5 ul are available and can be used depending on the application needs other types may be introduced later easy front access to lamp and flow cell for fast replacement electronic identification of flow cell and lamp with RFID Radio Frequency Identification tag for unambiguous identification lamp information part number serial number production date ignitions burn time cell information part number serial number production date nominal path length volume maximum pressure built i
107. ested actions 1 ProbableCause Exchange the filter motor or optical unit 2 ProbableCause Exchange the filter position sensor or optical unit 3 Defective main board Exchange the VWM board 1200 Series VWD Service Manual 139 10 Test Functions for Service Test Chromatogram A pre defined test chromatogram on the VWM board is processed through ADC like normal signals from the diodes and can be used to check the ADC and the data handling system The signal is available at the analog output and on the GPIB The Test Chromatogram is available in Agilent Diagnostic Software preferred tool Agilent Instant Pilot G4208A via Service Mode command Agilent ChemStation via command The run time of the chromatogram is depending on the setting for response time peak width If no stop time is set the chromatogram will repeat continuously VWAD1 AP Absorbance G1314E DEF AULT D 0 5 1 15 2 minj Figure 57 Test Chromatogram response time 2 seconds 140 1200 Series VWD Service Manual Test Functions for Service 10 When to do the Test In case the signal path should be checked to Control Software or data systems Interpreting the Results If the Control Software and or the data system shows the chromatogram the signal path is working Test Chromatogram with Instant Pilot The test can be started via the command line To start the test SIMU DFLT Reply RA 00000 SIMU DFLT
108. evaluation 29 control module G1323B 10 Control Module changing serial number and type 222 recover type 228 correction factors for flow cells 97 cuvette holder 173 parts 244 D DAC test 134 damaged packaging 34 data rate sampling rate 28 data recovery card DRC 28 DRC 86 declaration of conformity 308 delivery checklist 35 detection type 28 detector error messages 114 diagnostic additional signals 146 148 1200 Series VWD Service Manual Index baseline problem due to lamps 142 signals 102 142 software 106 test functions 123 133 wander and drift 144 warm up of detector 144 diagram main board 280 dimensions 27 DRC data recovery card 28 runrecovery 86 drift diagnostic 144 initial 79 E electrical connections descriptions of 17 electrostatic discharge ESD EMF early maintenance feedback 21 setting limits 22 using counters 22 160 181 environment 26 error message ADC hardware error 119 error messages compensation sensor open 112 detector 114 filter check failed 118 filter missing 118 general 109 grating missing 119 no run data available in device 122 error messages calibration failed 116 compensation sensor short 112 fan failed 113 grating filter motor defective 117 1200 Series VWD Service Manual heater current missing 115 heater power at limit 121 heater failed 121 holmium oxide test failed 117 ignition without cover 113 illegal value from air inlet sensor 120 i
109. evices that do not support auto negotiation the LAN interface will configure itself for 10 or 100 Mbps half duplex operation For example when connected to a non negotiating 10 Mbps hub the LAN interface will be automatically set to operate at 10 Mbps half duplex If the module is not able to connect to the network through auto negotiation you can manually set the link operating mode using link configuration switches on the module Table 10 Link Configuration Switches SW3 SW4 SW5 Link Configuration OFF speed and duplex mode determined by auto negotiation ON OFF OFF manually set to 10 Mbps half duplex ON OFF ON manually set to 10 Mbps full duplex ON ON OFF manually set to 100 Mbps half duplex ON ON ON manually set to 100 Mbps full duplex 1200 Series VWD Service Manual 53 4 LAN Configuration Automatic configuration with Bootp 54 When automatic configuration with Bootp is selected and the LAN interface is powered on it broadcasts a BOOTP Bootstrap Protocol request that contains its MAC hardware address A BOOTP server daemon searches its database for a matching MAC address and if successful sends the corresponding configuration parameters to the detector as a BOOTP reply These parameters become the active TCP IP parameters immediately and the TCP IP connection can be established Configuring the Agilent Bootp service program All examples shown in this chapter will not work in your environment
110. fer crystals After the installation of normal phase Hexane 5 Isopropanol Good wetting properties seals P N 0905 1420 The pump should never be used for priming empty tubings never let the pump run dry Use a syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump 1 Open the purge valve of your pump by turning it counterclockwise and set flow rate to 3 5 ml min 2 Flush all tubes with at least 30 ml of solvent 3 Setflow to required value of your application and close the purge valve Pump for approximately 10 minutes before starting your application 1200 Series VWD Service Manual 69 5 70 Using the Detector Requirements and Conditions What You Will Need The table below lists the items you need to have for the set up of the analysis Some of these are optional not required for the basic system Table 13 What you will need 1200 system Column Standard Pump plus degassing Autosampler Detector standard flow cell installed Degasser optional Column Compartment optional Agilent ChemStation B 03 02 SR1 or later or Instant Pilot G4208 B 02 07 or later optional for basic operation System should be correctly set up for LAN communication with the Agilent ChemStation Zorbax Eclipse XDB C8 4 6 x 150 mm 5 um Part No 993967 906 Part No 01080 68704 0 15 wt dimethylphthalate 0 15 wt diethylphthalate
111. formation on parts for maintenance ORE Agilent Technologies 233 14 Parts and Materials for Maintenance Overview of Maintenance Parts Table26 Maintenance Parts Item Description Part Number Cable CAN assembly 0 5 m 5181 1516 Cable CAN assembly 1 m 5181 1519 Deuterium lamp with RFID tag 61314 60100 Deuterium lamp without RFID tag 61314 60100 Standard flow cell with RFID tag 10 mm 14 ul additional flow cell G1314 60186 parts see Standard Flow Cell 10 mm 14 ul on page 236 Micro flow cell with RFID tag 3 mm 2 ul additional flow cell 61314 60187 parts see Micro Flow Cell 3 mm 2 pl on page 238 Semi micro flow cell with RFID tag 6 mm 5 ul additional flow cell G1314 60183 parts see Semi micro Flow Cell 6 mm 5 ul on page 240 High pressure flow cell with RFID tag 10 mm 14 ul additional 61314 60182 flow cell parts see High Pressure Flow Cell 10 mm 14 yl on page 242 Cuvette Holder see Cuvette Holder on page 244 61314 60200 Front cover 5065 9982 Leak handling parts see Leak Parts on page 245 234 1200 Series VWD Service Manual Parts and Materials for Maintenance 14 Accessory Kit This kit contains some accessories and tools needed for the installation and repair of the detector Table 27 Accessory Kit Parts Description Part Number Accessory kit G1314 68705 Corrugated tubing to waste re order b m 5062 2463 Peek outlet capillary kit i d is 0 25 mm PEEK 5062
112. g leak handling system 1 6 exchanging flow cell 168 exchanging lamps 165 for parts see parts for maintenance 233 introduction 157 overview 164 replacing firmware 177 simple repairs 158 standard flow cell 170 using the cuvette holder 173 manual configuration of LAN 59 message ADC hardware error 119 calibration failed 116 calibration lost 118 compensation sensor open 112 compensation sensor short 112 fan failed 113 filter check failed 118 filter missing 118 grating missing 119 1200 Series VWD Service Manual Index grating filter motor defective 117 heater current missing 115 heater failed 121 heater power at limit 121 holmium oxide test failed 117 ignition without cover 113 illegal value from air inlet senso 120 illegal value from heater sensor 120 lamp current missing 114 lamp ignition failed 115 lamp voltage missing 114 leak sensor open 111 leak sensor short 112 leak 111 meaning of 108 no run data available in device 122 remote timeout 110 shutdown 109 synchronization lost 110 timeout 109 wavelength check failed 118 method load 75 mirror contaminated 200 noise short term 28 0 online plot 78 spectra 84 operating temperature 27 optical unit parts 250 removing 201 stepper motor 16 optimization detector performance 94 1200 Series VWD Service Manual of the system 72 overview optical path optical system system overview 12 278 12 278 12 278 P parameters
113. gnostics Tests anymore Then the Agilent Diagnostic Software must be used instead The Agilent ChemStation may not include any maintenance test functions Table 19 Available Tests versus Interfaces Interface Test Diagnostic Software Agilent ChemStation Instant Pilot G4208A Wavelength yes Tests Maintenance Verification Re calibration Lamp Intensity yes Tests Diagnosis Holmium Test yes Tests Diagnosis Cell Test yes Tests n a D A Converter Test yes Tests n a Filter Grating Motor Test yes Tests command line Test Chromatogram yes command line command line Spectrum Blank Sample Holmium yes n a Control Service Dialog for service only n a for service only interface provides passed fail information or a plot requires a comand via comand line requires a comand via comand line in Service Mode 1200 Series VWD Service Manual 105 7 Troubleshooting and Diagnostics Agilent Diagnostic Software The Agilent Diagnostic Software is a standalone product that can be used with or without data system Agilent Lab Advisor helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor provides diagnostic capabilities for all Agilent 1200 Series HPLC modules This includes tests and calibrations procedures as well a
114. gth scan Recorder integrator 100 mV or 1 V output range 0 001 2 AU one output LAN card integrated on main board Controller area network CAN RS 232C APG Remote ready start stop and shut down signals 1200 Series VWD Service Manual 29 2 30 Site Requirements and Specifications Table 4 Performance Specifications G1314D VWD and G1314E VWD SL Plus Type Specification Comments Safety and maintenance GLP features Housing Extensive diagnostics error detection and display through control module and Agilent ChemStation leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage in terms of lamp burn time with user settable limits and feedback messages Electronic records of maintenance and errors Verification of wavelength accuracy with built in holmium oxide filter All materials recyclable 1200 Series VWD Service Manual Site Requirements and Specifications 2 Specifications ASTM Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid Chromatography Reference conditions Standard flow cell path length 10 mm flow 1 ml min LC grade methanol Noise 0 15 x 10 AU at 230 nm TC 2 s 0 25 x 10 AU at 254 nm RT 2 s ASTM RT 2 2 TC Linearity Linearity is measured with caffeine
115. he beam through the flow cell is absorbed depending on the solutions in the cell in which UV absorption takes place and the intensity is converted to an electrical signal by means of the sample photodiode Part of the light is directed to the reference photodiode by the beam splitter to obtain a reference signal for compensation of intensity fluctuation of the light source A slit in front of the reference photodiode cuts out light of the sample bandwidth Wavelength selection is made by rotating the grating which is driven directly by a stepper motor This configuration allows fast change of the wavelength The cutoff filter is moved into the lightpath above 370 nm to reduce higher order light Deuterium lamp P Lens Mirror M1 Sample diode Flow cell Grating Mirror M2 Beam splitter Reference diode Figure 1 Optical Path of the Variable Wavelength Detector 12 1200 Series VWD Service Manual Introduction to the Variable Wavelength Detector 1 Flow Cell A variety of flow cell cartridges can be inserted using the same quick and simple mounting system For the G1314D VWD and G1314E VWD SL Plus the flow cells have an integrated RFID tag that contains the flow cell specific information e g part number cell volume path length A RFID tag reader reads out this information and transfers it to the user interface Ww ON RFID tag SN Y 7 4 A DA gt G4 5 Figure 2 Flow Cell with RFID tag Table1 F
116. ice Manual Using the Detector 5 Scanning with the VWD Access to the scan feature is only possible during run 1 Set up a run Start a run While running on the baseline select from the menu Instrument gt More VWD gt Blank Scan A background scan is stored in the memory System 2 online Method amp Run Control File RunControl Instrument Method Sequence View Abort Help iy 8 Meu Set up WD Signals mz 5 Step 1 Blank Scan scan of the background solvent is stored in the memory Step 2 Sample Scan scan of the peak of interest is PONE i taken while the peak stays in the flow cell stop flow Snapshot jaurati condition Boy atl TU Online Spectrum Sample Scan minus Blank Scan sii AFCDE System Off Gl BATCH 8 DEF L Revisions amp Serial s x DGNOT Columns i INSTPE E LOADT Configure 1100 1200 Access S 2 ml min 4 When the peak of interest enters the flow cell stop the flow set flow rate to Zero or open the purge valve and wait a few moments to stabilize the concentration Turning off the pump would stop the run and no access to the sample scan is possible 5 Select from the menu Instrument gt More VWD gt Sample Scan A sample scan is taken in the range defined under Special Setpoints on page 90 and the Online Spectra window see Online Spectra on page 84 displays the result Sample Scan minus Blank Scan 1200 Series VWD Se
117. indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 302 1200 Series VWD Service Manual Appendix 18 Radio Interference Cables supplied by Agilent Technoligies are screened to provide opitimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 1200 Series VWD Service Manual 303 18 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A At Operator Position Normal Operation According to ISO 7779 1988 EN 27779 1991 Type Test 304 1200 Series VWD Service Manual Appendix 18 UV Radiation Emissions of ultraviolet radiation 200 315 nm from this product is limited such that radiant exposure incident upon the unprotected skin or eye of operator or service personnel is limited to the following TLVs Thre
118. installation of older firmware might be necessary to keep all systems on the same validated revision or ifanew module with newer firmware is added to a system or ifthird part control software requires a special version LAN RS 232 Firmware Update Tool or Agilent Lab Monitor amp Diagnostic Software LMD Instant Pilot G4208A only if supported by module Control Module G1323B only if supported by module Description Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware the following steps have to be performed 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 Load the firmware into the module as described in the documentation The initial firmware revision of the G1314D VWD and the G1314E VWD SL Plus is B 06 20 main and resident No further downgrade is possible Due to a different hardware platform there is no way to convert a G1314D VWD and the G1314E VWD SL Plus to a G1314A B VWD or G1314C VWD SL 1200 Series VWD Service Manual 177 12 Maintenance Replacing the Module s Firmware 178 1200 Series VWD Service Manual 1200 Series VWD Service Manual 13 Repair Cautions and Warnings 180 Removing the Top Cover and Top Foam 182 Locate the Main Assemblies 185 Re
119. into the slot and tighten the cell screws both parallel until the mechanical stop 3 Assemble the column detector capillary Depending on 4 Connectthe newly assembled fitting of the capillary to the flow cell type it may be a PEEK or SST capillary the inlet connector 1200 Series VWD Service Manual 43 3 Installing the Detector 5 Connect the other end of the capillary to the column 6 Connect the PEEK waste capillary to the outlet connector 7 Establish a flow and observe for leakage 8 Replace the front cover The installation of the detector is now complete The detector should be operated with the front cover in place to protect the flow cell area against strong drafts from the outside 1200 Series VWD Service Manual 1200 Series VWD Service Manual 4 LAN Configuration What you have to dofirst 46 TCP IP parameter configuration 47 Configuration Switches 48 Initialization mode selection 49 Link configuration selection 53 Automatic configuration with Bootp 54 Storing the settings permanently with Bootp 58 Manual Configuration 59 PC and Agilent ChemStation Setup 64 This chapter provides information on connecting the detector to the Agilent ChemStation PC RE Agilent Technologies 45 4 LAN Configuration What you have to do first The module has an on board LAN communication interface
120. itch is pressed and the green indicator lamp is illuminated The detector is turned off when the line power switch is protruding and the green light is off To disconnect the detector from line unplug the power cord The power supply still uses some power even if the power switch at the front panel is turned off 1200 Series VWD Service Manual 41 3 Installing the Detector Flow Connections to the Detector Tools required Parts required Preparations Two wrenches 1 4 5 16 inch for capillary connections Description Other modules Parts from accessory kit see Table 27 on page 235 Detector is installed in the LC system Toxic and hazardous solvents The handling of solvents and reagents can hold health risks gt When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 42 The flow cell is shipped with a filling of isopropanol also recommended when the instrument and or flow cell is shipped to another location This is to avoid breakage due to subambient conditions 1200 Series VWD Service Manual Installing the Detector 3 1 Press the release buttons and remove the front cover to 2 Remove the metal cover and install the flow cell Press have access to the front area the flow cell completely
121. k sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Exchange the leak sensor Compensation Sensor Open The ambient compensation sensor NTC on the VWM board in the detector has failed open circuit The resistance across the temperature compensation sensor NTC on the VWM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective VWM board Exchange the VWM board Compensation Sensor Short The ambient compensation sensor NTC on the VWM board in the detector has failed short circuit The resistance across the temperature compensation sensor NTC on the VWM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective VWM board Exchange the VWM board 1200 Series VWD Service Manual Error Information 8 Fan Failed The cooling fan in the detector has failed The hall sensor on the fan shaft is used by the VWM board to monitor the fan speed
122. l min ee 01 5 ml min Internal column diameter 10mm 21mm 30mm 46mm Figure 48 Choosing a Flow Cell Standard HPLC Applications Ultra fast separation with RRLC systems Column ID 2 1 mm 3 0 mm 4 6 mm Configuration No damper Damper Damper No mixer Mixer Mixer Tt Flow cell 2 ul 3 mm 5 ul 6 mm 14 ul 10 mm Figure 49 Choosing a Flow Cell for G1314E for ultra fast separation with RRLC systems e For ultra fast analysis with step gradients the micro flow cell 2 ul 3 mm gives the best performance e In high resolution analysis time is not the highest priority Higher delay volumes are accepted Therefore we recommend to use the damper plus mixer for a highest signal to noise Iflonger columns gt 50 mm for higher resolution are used then the next larger flow cell is the preferred choice for higher sensitivity 1200 Series VWD Service Manual 95 96 How to optimize the detector Flow Cell Path Length Lambert Beer s law shows a linear relationship between the flow cell path length and absorbance Absorbance log T log _e C d where T is the transmission defined as the quotient of the intensity of the transmitted light divided by the intensity of the incident light lg e is the extinction coefficient which is a characteristic of a given substance under a precisely defined set of conditions of wa
123. latile memory of the detector It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters given that the initialization mode selection switches are allowing it Non Volatile RAM Stored Parameter Ld Control Module Figure 20 Manual Configuration Principle With Telnet Whenever a TCP IP connection to the module is possible TCP IP parameters set by any method the parameters may be altered by opening a Telnet session 1 Open the system DOS prompt window by clicking on Windows START button and select Run Type cmd and press OK 2 Type the following at the system DOS prompt 1200 Series VWD Service Manual 59 4 LAN Configuration c gt telnet lt IP address gt or c gt telnet host name gt WINDOWS system32 cmd exe C gt telnet 134 40 27 104 Figure 21 Telnet Starting a session where IP address may be the assigned address from a Bootp cycle a configuration session with the Handheld Controller or the default IP address see Configuration Switches on page 48 When the connection was established successfully the module responds with the following c Telnet 134 40 27 104 Agilent Technologies Gi314E DE81900662 gt Figure 22 A connection to the module is made 3 Type and press enter to see the available commands cx Telnet
124. le to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the detector The female end of the power cords is identical It plugs into the power input socket at the rear of the detector The male end of each power cord is different and designed to match the wall socket of a particular country or region The absence of ground connection and the use of an unspecified power cord can lead to electric shock or short circuit Electric Shock gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1200 Series VWD Service Manual 25 2 Site Requirements and Specifications Bench Space The detector dimensions and weight see Table 3 on page 27 allows you to place the detector on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches
125. llegal value from heater sensor 120 amp current missing 114 amp ignition failed 115 amp voltage missing 114 eak sensor open 111 eak sensor short 112 eak 111 remote timeout 110 shutdown 109 synchronization lost 110 timeout 109 wavelength check failed 118 ESD protection 162 exchanging parts see repairs 179 external contact cable 261 F fan parts 252 features GLP 30 instrument layout 20 main board 279 safety and maintenace 30 firmware description 285 main system 285 minimum revision 39 resident system 285 update tool 286 updates 177 286 upgade downgrade 177 flow cell micro parts 238 flow cell configuration 83 flow cell correction factors 97 high pressure parts 242 semimicro parts 240 flow cells matching for application 95 flow cell standard parts 236 types and data 29 foams parts 25 7 Forced Cold Start settings 296 fuses none in the instrument 298 G general error messages 109 GPIB cable 261 interface 289 grating contaminated 200 motor test 136 H heater parts 252 holmium oxide declaration of conformity 308 test 130 information 311 Index onsound emission 304 on UV radiation 305 on cuvette holder 173 onsolvents 306 initialization mode selection 49 installation bench space 26 damaged packaging 34 of flow connections 42 ofthe detector 39 site requirements 24 24 Instant Pilot G4208A 10 Instant Pilot changing serial number and type 221 recover
126. low Cell Data STD Semi micro Micro High Pressure Maximum pressure 40 4 40 4 120 12 400 40 bar Path length 10 conical 6 conical 3 conical 10 conical mm Volume 14 5 2 14 yl Inlet i d 0 17 0 17 0 12 0 17 mm Inlet length 750 750 310 310 mm Outlet i d 0 25 0 25 0 17 0 25 mm Outlet length 120 120 120 120 mm Materials in contact with solvent SST quartz PTFE PEEK SST quartz PTFE SST quartz PTFE SST quartz Kapton 1200 Series VWD Service Manual 13 1 Introduction to the Variable Wavelength Detector Lamp The light source for the UV wavelength range is a deuterium lamp As a result of plasma discharge in a low pressure deuterium gas the lamp emits light over the 190 to 600 nm wavelength range For the G1314D VWD and G1314E VWD SL Plus the lamp has an integrated RFID tag that contains the lamp specific information e g part number burn time A RFID tag reader reads out this information and transfers it to the user interface Source Lens Assembly The source lens receives the light from the deuterium lamp and focuses it onto the entrance slit Entrance Slit Assembly The entrance slit assembly has an exchangeable slit The standard one has a 1 mm slit For replacement and calibration purposes to optimize the alignment a slit with a hole is needed 14 1200 Series VWD Service Manual Introduction to the Variable Wavelength Detector 1 Filter Assembly The filter assembly is ele
127. ltages for the other electronic components will be turned off after 30 seconds The status lamp will light up red and an error will be logged into the logbook of the user interface Always operate the detector with the top covers in place Toxic and hazardous solvents The handling of solvents and reagents can hold health risks When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 180 1200 Series VWD Service Manual Repair 13 CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD can damage electronic boards and components 2 In order to prevent damage always use ESD protection when handling electronic boards and components CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD gt To prevent accidental electrostatic discharge when coming into contact with components inside the instrument touch one of the metal housing panels at the front of the instrument WARNING Eye damage by detector light Eye damage may result from directly viewing the light produced by the deuterium lamp used in this product gt Always turn the deuterium lamp off before removing it 1200 Series VWD Service Manual 181 13 Repair Removing the T
128. mperature DAII U609 SWA DEF LC 2008 08 21 10 39 34WV 0110 D 494 r r T r r T r r T r r T r r r T r r 20 40 60 80 100 mir VWD3 G Optical Unit Temperature DAII D609 9vA DEF LC 2008 08 21 10 39 34 NV 0110 D J 415 i r 4 60 80 100 miri VWDS3 O Aux 1 DAII 0609 9WA DEF LC 2008 08 21 10 39 34 V 0110 D ents 95000 90000 85000 P 80000 75000 T T T T T 60 80 100 min 20 40 VWD3 P Aux 2 DAII 0609 9A DEF LC 2008 08 21 10 39 34 V 0110 D ents 28000 37600 Wall ana t 27250 hi ius iad WIN aa itary 26750 r r 20 40 60 80 Figure 64 Example plot from ChemStation 156 1200 Series VWD Service Manual 1200 Series VWD Service Manual 11 Maintenance and Repair Introduction to Maintenance and Repair 158 Warnings and Cautions 159 Cleaning the Detector 161 Using the ESD Strap 162 This chapter provides general information on maintenance and repair of the detector E Agilent Technologies 157 11 Maintenance and Repair Introduction to Maintenance and Repair 158 Simple Repairs Maintenance The detector is designed for easy repair The most frequent repairs such as lamp change and flow cell change can be done from the front of the detector with the detector in place in the system stack These repairs are described in chapter Maintenance part of the User Manual and Service Manual Exchanging Internal Parts Repair
129. n Do not run shorter runs than 60 minutes Typical effects from air condition systems run at cycles of 15 to 20 minutes 1200 Series VWD Service Manual 145 10 Test Functions for Service Diagnostic Signal Parameters Below the details of the available diagnostic signals is described Table 20 Signal Parameters Signal Description 5 main board temperature 6 optical unit temperature 7 UV lamp anode voltage You may use just a single signal or a combination of 2 or 3 separated by comma Table 21 Diagnostic Signals Chemstation Commands Command Description print sendmodule nvwd RAWD SIGSTOR ADD 5 6 7 to store the diagnostic signals together with the normal signal print sendmodule nvwd RAWD SIGSTOR REM 5 6 7 to de activate the storage if no longer used print sendmodule nvwd RAWD CSIGMON ADD 5 6 7 to activate the diagnostic signals in the Online Plot window print sendmodule nvwd RAWD CSIGMON REM 5 6 7 to de activate the diagnostic signals in the Online Plot window if no longer used If the diagnostic signals are not de activated by command they will be active until the detector is power cycled 146 1200 Series VWD Service Manual Test Functions for Service 10 Diagnostic Signals Evaluate the Data 1 When the run has completed open the ChemStation Data Analysis 2 Load the data file 3 Look for a correlation between the temperature or lamp voltage signal and the baseline 4 Det
130. n electronic temperature control ETC for improved baseline stability and built in holmium oxide filter for fast wavelength accuracy verification higher data rate up to 160 Hz for ultra fast HPLC G1314E VWD SL Plus see Peakwidth Settings on page 91 data recovery card DRC gives unique data never lost insurance G1314E VWD SL Plus see Run Recovery G1314E only on page 86 For specifications refer to Table 4 on page 28 Two version of the Agilent 1200 Series variable wavelength detector are available G1314D VWD standard version G1314E VWD SL Plus adds high data rates for RRLC Rapid Resolution LC and unique data never lost insurance 1200 Series VWD Service Manual Introduction to the Variable Wavelength Detector 1 Introduction to the Detector NOTE The G1314D VWD and G1314E VWD SL Plus cannot be operated with a G1323B Control Module Use the Instant Pilot G4208A as local controller 1200 Series VWD Service Manual 11 1 Introduction to the Variable Wavelength Detector Optical System Overview The optical system of the detector is shown in the figure below Its radiation source is a deuterium arc discharge lamp for the ultraviolet UV wavelength range from 190 to 600 nm The light beam from the deuterium lamp passes through a lens a filter assembly an entrance slit a spherical mirror M1 a grating a second spherical mirror M2 a beam splitter and finally through a flow cell to the sample diode T
131. n the user interface Recover with Agilent Diagnostic Software Type amp Firmware Agilent Diagnostic Software in CE mode If no LAN connection is possible use RS 232 The example uses G1315B as correct type The module must be configured to Stay Resident Mode module boots in resident mode flashing status LED Aa oc N oo N c ol Open a connection to the module or via system Select Board Check and Change and press Start In the type field enter G1315B and press Apply After a power cycle the module should show up with the correct product number type in the user interface Load the correct main firmware into the module Turn the module OFF Set module s configuration switch back to normal mode Turn on the module If required load final firmware into the module 1200 Series VWD Service Manual Repair 13 Recover with Agilent ChemStation 1 Use the ChemStation command line to change to TYPE product number as described under Using the Agilent ChemStation on page 219 After power cycle of module the correct TYPE shows up Recover with Instant Pilot USB Flash Drive with file CUSTINST CMD and a PC with USB interface The example uses G1315B as correct type and G1319B as incorrect type 1 c RC N Edit create the file CUSTINST CMD and add the following line XXXXX Command from USB Type Change TYPE YYYYYY where XXXXX is for example G1319 from the mis typed G1319B and YYYYYY is the
132. nce Specifications 28 This chapter gives information on environmental requirements physical and performance specifications XE Agilent Technologies 23 2 Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Consideration The detector power supply has wide ranging capabilities see Table 3 on page 27 It accepts any line voltage in the above mentioned range Consequently there is no voltage selector in the rear of the detector There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Instrument is partially energized when switched off The power supply still uses some power even when the power switch on the front panel is turned OFF Repair work at the detector can lead to personal injuries e g shock hazard when the detector cover is opened and the instrument is connected to power gt To disconnect the detector from the power line unplug the power cord Incorrect line voltage at the instrument Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage 24 1200 Series VWD Service Manual CAUTION Site Requirements and Specifications 2 Unaccessable power plug In case of emergency it must be possib
133. normal This results in low photo current readings on sample and reference side Contaminated mirrors and the grating result into a replacement of the optical unit 200 1200 Series VWD Service Manual Repair 13 Removing the Optical Unit When Tools required Parts required Preparations When other assemblies have to be removed or when defective Screwdriver POZI 1 PT3 Part number Description 1 G1314 69062 Optical unit G1314D E exchange part 1 G1314 60062 Optical unit G1314D E Turn OFF the detector Disconnect the power cable Disconnect capillaries Remove detector from the system and place it on the working bench Remove the Front Panel the Closure the Metal Cover and the Top Foam see Removing the Top Cover and Top Foam on page 182 Remove the deuterium lamp if optical unit will be replaced Remove the flow cell For ESD information refer to Cautions and Warnings on page 180 1200 Series VWD Service Manual 201 13 Repair Removing the Optical Unit 1 Disconnect all cables from the main board 2 To disconnect the flat cables from its connectors lift the black holder of the connector using a small flat screw driver as shown in the bottom connector ugvuvu SA 3 4 Remove the Heater Assembly and the Bracket that secures the optical unit 202 1200 Series VWD Service Manual Repair 13 Removing the Optical Unit 5 Disconnect the deuterium lamp at the l
134. nsity Test with Instant Pilot Intensity Test Failed Probable cause 1 Empty flow cell 2 Flow cell windows dirty Passed Y Suggested actions Ensure the flow cell is filled with water Repeat the test with the flow cell removed If the test passes exchange the flow cell windows 3 Optics defect 4 Lamp defectiv 1200 Series VWD Service Manual Clean replace optical components Exchange the lamp 125 9 Test Functions for User Checking the Photocurrent 126 To identify problems with the flow cell contaminated windows air bubbles and so on you can use raw data from the sample and reference photodiode unfiltered and not logarithmic The photocurrent information RAW SAMPLE REFERENCE is available in Agilent Diagnostic Software preferred tool Agilent Instant Pilot G4208A via More Diagnosis VWD Lamp Intensity Test Photocurrent Evaluation With a clean cell the counts for sample and reference are in the same range If the sample side shows much lower values than the reference side the flow cell might have a problem due to v dirty windows Y absorbing solvents compounds Y not correctly mounted flow cell or v air bubbles 1200 Series VWD Service Manual Test Functions for User 9 Checking the Photocurrent Checking the Photocurrent with the Instant Pilot Diagnosis Intensity VWD SL Max intens gt 320 000 cts measured 6039917 Passed Min intens gt 6 40
135. nt damage always use an ESD strap when handling electronic boards and components 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 65 Using the ESD Strap 1200 Series VWD Service Manual 1200 Series VWD Service Manual e e o ee 12 d Maintenance e Overview of Maintenance 164 ExchangingaLamp 165 Exchanging a Flow Cell 168 Repairing the Flow Cells 170 Using the Cuvette Holder 173 Correcting Leaks 175 Replacing Leak Handling System Parts 176 Replacing the Module s Firmware 177 This chapter describes the maintenance of the detector RE Agilent Technologies 163 12 Maintenance Overview of Maintenance The following pages describe maintenance simple repairs of the detector that can be carried out without opening the main cover Table 22 Simple Repairs Procedures Typical Frequency Notes Deuterium lamp If noise and or drift exceeds your application limits or A VWD test should be performed after exchange lamp does not ignite replacement Flow cell exchange If application requires a different flow cell type A VWD test should be performed after replacement Cleaning flow cell parts If leaking or if intensity drops due to contaminated flow A pressure tightness test should be
136. o the Agilent 1200 Series modules The other end depends on the instrument to be connected to Agilent 1200 to General Purpose ConnectorG1351 81600 Wire Color Pin Agilent Signal Name BCD Digit 1200 Green 1 BCD 5 20 Violet 2 BCD 7 80 uu Blue 3 BCD 6 40 XE Yellow 4 BCD 4 10 SS Black 5 BCD 0 1 ay Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 270 1200 Series VWD Service Manual Agilent 1200 to 3396 Integrators Identifying Cables 16 Connector03396 60560 Pin 3392 3 Pin Agilent Signal Name BCD Digit 1200 1 1 BCD 5 20 2 2 BCD7 80 Be 3 3 BCD 6 40 4 4 BCD 4 10 5 5 BCDO 1 1e 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 271 1200 Series VWD Service Manual 16 Identifying Cables External Contact Cables 56 C Oo 1 10 lt 6 1p 905 11 One end of this cable provides a 15 pin plug to be connected to Agilent 1200 Series module s interface board The other end is for general purpose Agilent 1200 Series Interface Board to general purposes ConnectorG1103 61611 Color Pin Agilent Signal Name 1200 White 1 EXT 1 z Brown 2 EXT 1 Green 3 EXT 2 res Yellow 4 EXT2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected
137. oam Parts Item Description Part Number 1 2 EPP foam kit includes 61314 60009 Base and top part 1 and 2 3 Damper kit includes bumpers for optical and fan 61314 67001 Do not order the individual part numbers mentioned on the foam Figure 82 Foam Parts 1200 Series VWD Service Manual 257 15 Parts for Repairs Power and Status Light Pipes Table 41 Power and Status Light Pipes Item Description Part Number Power supply assembly 0950 2528 Screw M4 x 0 7 8 mm lg to fix power supply at rear panel 0515 0910 Washer 2190 0409 1 Power light pipe 5041 8382 2 Status light pipe 5041 8384 3 Power switch button 5041 8381 4 Coupler for power supply actuator 5041 8383 Figure 83 Power and Status Light Pipe 258 1200 Series VWD Service Manual 1200 Series VWD Service Manual 16 Identifying Cables Cable Overview 260 Analog Cables 262 Remote Cables 265 BCD Cables 270 External Contact Cables 272 CAN LAN Cables 273 Auxiliary Cables 274 RS 232 Cables 275 This chapter provides information on cables used with the 1200 series of HPLC modules RE Agilent Technologies 259 16 Identifying Cables Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Type Description Part Number Analog cables 3390 2 3 integrators 01040 60101 3394 6 integrators 35900 60750 Agilent 35900A A D converter 3
138. ogbook shows an entry No Run data available in device 1200 Series VWD Service Manual Using the Detector 5 Automated Run Recovery in case of temporary communication failures Table 15 Automated Run Recovery in case of temporary communication failures Situation Reaction On ChemStation All OK Run in progress Data Analysis wo Status ee Reda O Elapsed run time is running Spectra counter is running Wavelength 254 nm Data are stored on PC and on card LAN breaks Runin progress Data Analysis mE Run Rawdata Fondata MENENS Error Power Fail Elapsed run time stops Spectra counter stops Data continues to be stored on card LAN recovers Runin progress Data Analysis WWD Status Run Rawdata Rawdata Error Power Fail cleared Elapsed run time continues at actual time Wavelength 254 nm Spectra counter continues Data continues to be stored on PC and on card ChemStation tries already to add missing data depends on the data load Stop time elapsed Runin progress Data Analysis Prerun Rawdata O Pem Rawdata i Elapsed run time stops Spectra counter continues Wavelength 254 nm ChemStation continues to add missing data Run ends Ready Run finished O Pien Ready Prerun Ready Wavelength 254 nm If the detector status window is not opened you will realize only the Power Fail error and the long
139. ognized by the system 1200 Series VWD Service Manual Error Information 8 Leak A leak was detected in the detector The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the VWM board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Leaking flow cell Exchange flow cell components Leak Sensor Open The leak sensor in the detector has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the VWM Ensure the leak sensor is connected correctly board 2 Defective leak sensor Exchange the leak sensor 1200 Series VWD Service Manual 111 112 Error Information Leak Sensor Short The leak sensor in the detector has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the lea
140. on that cannot take the data from the detector in time or alost network connection no application data of the detector is lost The G1314E VWD SL Plus must be operated with the CompactFlash card when the unique data never lost insurance is used 284 1200 Series VWD Service Manual Hardware Information 17 Firmware Description The firmware of the instrument consists of two independent sections anon instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1200 series modules Its properties are the complete communication capabilities CAN LAN and RS 232C memory management ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN and RS 232C memory management ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like run synchronization through APG remote error handling diagnostic functions or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 1200 Series VWD Service Manual 285 17 Hardware Information 286 Firmware Updates Firmware updates can be done using your user interfa
141. on of the detector it goes through a cycle of different states 0 to 0 5 minutes the heater control is OFF and the heater element runs at 0 6 duty cycle 0 5 to 1 minutes the heater control is OFF and the heater element runs at 66 6 duty cycle This first minute is used as self test of the heater functionality e 1 to 30 minutes the heater control is OFF and the heater element runs at 40 duty cycle after 30 minutes the heater control is ON and is working with optimized parameters to get the optical unit into the optimal temperature window stabilized 1200 Series VWD Service Manual Test Functions for Service 10 The times to stabilize the baseline may vary from instrument to instrument and depends on the environment The example below was done under stable environmental conditions VIDA A Abtorbance 633380 UIDARD TE MP 2 D mau E va 03 P a 02 md e a E MM M T A MWD1 6 Optical Unit Temperatew 13 14DEBOARD TEMP 2 0 2 heater control OFF heater control ON 10 20 30 2 min Figure 58 Example of warm up cycle and corresponding diagnostic signals Setup of Detector 1 Remove the flow cell to eliminate problems from the flow system 2 Use the following detector default settings Default LC method 3 Setup a blank run with a run time of at least one hour 4 Type one of the commands described in Table 21 on page 146into the ChemStation command line or create a ChemStation macro 5 Start the ru
142. op Cover and Top Foam When For all repairs inside the detector Tools required Screwdriver POZI 1 PT3 Parts required Description Depends on the work inside and the following procedures Preparations Turn OFF the detector Disconnect the power cable Disconnect capillaries Remove detector from the system and place it on the working bench 1 Press the release buttons and remove the front cover 2 Install the ESD strap Move the power lock across the Unclip and remove the waste funnel assembly power inlet ESD strap 182 1200 Series VWD Service Manual Repair 13 Removing the Top Cover and Top Foam 3 Lift the cover and slide it towards the rear 4 Unscrew the screws at the rear of the top plate slide the plate towards the front and remove it 5 Carefully remove the top foam from the detector First units have pre released foam parts Therefore a single piece of foam is located between optical unit and front sheet metal right of the flow cell to close the air flow 1200 Series VWD Service Manual 183 13 Repair Removing the Top Cover and Top Foam 6 Location from the pre release foam part 7 Remove the pre release foam part NOTE Do not connect a power plug to module after removing the top covers A safety light switch on the main board will turn off fan immediately and electronic after 30 seconds to avoid the operation with removed cover
143. or Status Indicator The detector status indicator indicates one of four possible detector conditions When the status indicator is OFF and power switch light is on the detector is in a prerun condition and is ready to begin an analysis A green status indicator indicates the detector is performing an analysis run mode A yellow indicator indicates a not ready condition The detector is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the detector has detected an internal problem which affects correct operation of the detector Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis A red blinking indicator indicates that the module is in resident mode e g during update of main firmware 1200 Series VWD Service Manual Troubleshooting and Diagnostics 7 Available Tests versus Interfaces Depending on the used interface the available tests and the screens reports may vary Preferred tool should be the Agilent Diagnostic Software see Agilent Diagnostic Software on page 106 Screenshots used within these procedures are based on the Agilent ChemStation In future a user interface may not show the Dia
144. osure the Metal Cover and the Top Foam see Removing the Top Cover and Top Foam on page 182 Remove the Heater Assembly see Replacing the Heater Assembly on page 195 196 1200 Series VWD Service Manual Repair 13 Replacing the Heater Interface Cable 1 Disconnect the cable from the connector on the Interface Board Opening Heater Lamp 2 Insert a flat screw driver between board and sheet metal and remove the board from its two plastic rivets they will break off and are not reusable 3 Usethe new rivets provided with the new Interface Board to fix the new board 4 Insert the first rivet through the hole and press the interface board across Redo with the second rivet 1200 Series VWD Service Manual 197 13 Repair Danlaninn tha Haatar Intarfana f 4 Replacing ne neater intemace van 5 Disconnect the cable at the connector on the Main Board 8 Insert the new cable into the foam Reconnect the close to the fan Pull the cable out of the foam connectors to the Interface Board and the Main Board 7 Reconnect the cable to the Interface Board 8 Next Steps Replace the Top Foam the Metal Cover the Closure and the Front Panel see Installing the Foam and the Top Cover on page 229 Replace the detector in the system 198 1200 Series VWD Service Manual Repair 13 Repairs in the Optical Unit Troubleshooting should
145. ote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained 1200 Series VWD Service Manual Hardware Information 17 by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal level are defined as standard TTL levels 0 V is logic true 5 V is false fan out is 10 input load is 2 2 kOhm against 5 V and output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 volt power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 V with respect to the ground
146. otp mode If you want to store your parameters permanently on the module for use without the Agilent Bootp service refer to Storing the settings permanently with Bootp on page 58 1200 Series VWD Service Manual 57 4 LAN Configuration Storing the settings permanently with Bootp 58 If you want to change parameters of the module using the Bootp follow the instructions below 1 2 Turn off the module Change the module s settings of the Configuration Switch to Bootp amp Store mode see Table 8 on page 49 Start the Agilent Bootp Service and open its window If required modify the parameters for the module according to your needs using the existing configuration Press OK to exit the Bootp Manager Now turn on the module and view the Bootp Server window After some time the Agilent Bootp Service will display the request from the LAN interface The parameters are now stored permanently in the non volatile memory of the module 7 Close the Agilent Bootp Service and turn off the module 8 Change the settings of the module s Configuration Switch to Using Stored mode see Table 8 on page 49 Power cycle the module The module can be accessed now via LAN without the Agilent Bootp Service refer to Agilent ChemStation Setup on page 65 1200 Series VWD Service Manual LAN Configuration 4 Manual Configuration Manual configuration only alters the set of parameters stored in the non vo
147. owing initialization init modes are selectable Table 8 Initialization Mode Switches SW6 SW7 SW8 Init Mode OFF OFF OFF Bootp OFF OFF ON Bootp amp Store OFF ON OFF Using Stored OFF ON ON Using Default Bootp When the initialization mode Bootp is selected the detector tries to download the parameters from a Bootp Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the module Therefore the parameters are lost with the next power cycle of the module Bootp gt Active Server Parameter Figure 11 Bootp Principle Bootp amp Store When Bootp amp Store is selected the parameters obtained from a Bootp Server become the active parameters immediately In addition they are stored to the non volatile memory of the module Thus after a power cycle they are still available This enables a kind of bootp once configuration of the module 1200 Series VWD Service Manual 49 4 50 LAN Configuration Example The user may not want to have a Bootp Server be active in his network all the time But on the other side he may not have any other configuration method than Bootp In this case he starts the Bootp Server temporarily powers on the module using the initialization mode Bootp amp Store waits for the Bootp cycle to be completed closes the Bootp Server and powers off the module Then he selects the initialization
148. placing the Temperature Sensor 186 Exchanging the Fan 190 Replacing the RFID Tag Reader Lamp or Flow Cell 192 Replacing the Heater Assembly 195 Replacing the Heater Interface Cable 196 Repairs in the Optical Unit 199 Removing the Optical Unit 201 Installing the Optical Unit 204 Exchanging the Leak Sensor 207 Replacing Status Light Pipe 209 Exchanging the Main Board and Power Supply 210 Changing the Type and Serial Number 217 Installing the Foam and the Top Cover 229 Assembling the Main Cover 231 This chapter gives instructions on how to repair the detector ORE Agilent Technologies 179 13 Repair Cautions and Warnings Module is partially energized when switched off as long as the power cord is plugged in Risk of stroke and other personal injury Repair work at the module can lead to personal injuries e g shock hazard when the module cover is opened and the instrument is connected to power gt Never perform any adjustment maintenance or repair of the module with the top cover removed and with the power cord plugged in The security lever at the power input socket prevents that the module cover is taken off when line power is still connected Never plug the power line back in when cover is removed The electronics of the detector will not allow operation of the detector when the top cover and the top foam are removed A safety light switch on the main board will inhibit the operation of the fan immediately Vo
149. ptical Unit 201 Installing the Optical Unit 204 Exchanging the Leak Sensor 207 Replacing Status Light Pipe 209 Exchanging the Main Board and Power Supply 210 Changing the Type and Serial Number 217 Installing the Foam and the Top Cover 229 Assembling the Main Cover 231 14 Parts and Materials for Maintenance 233 Overview of Maintenance Parts 234 Accessory Kit 235 Standard Flow Cell 10 mm 14 ul 236 Micro Flow Cell 3 mm 2 ul 238 Semi micro Flow Cell 6 mm b ul 240 High Pressure Flow Cell 10 mm 14 ul 242 Cuvette Holder 244 1200 Series VWD Service Manual Contents Leak Parts 245 15 Partsfor Repairs 247 Overview of Main Assemblies 248 Optical Unit 250 Heater and Fan 252 Sheet Metal Kit 254 Plastic Parts 255 Leak Parts 256 Foam Parts 257 Power and Status Light Pipes 258 16 Identifying Cables 259 Cable Overview 260 Analog Cables 262 Remote Cables 265 BCD Cables 270 External Contact Cables 272 CAN LAN Cables 273 Auxiliary Cables 274 RS 232 Cables 275 17 Hardware Information 277 Optical System Components 278 Detector Main Board 279 Firmware Description 285 Agilent 1100 1200 Series Interfaces 287 Setting the 8 bit Configuration Switch 293 Main Power Supply Assembly Standard 297 18 Appendix 299 General Safety Information 300 Radio Interference 303 Sound Emission 304 UV Radiation 305 Solvent Information 306 Declaration of Conformity for HOX2 Filter 308 6 1200 Series VWD S
150. r ADC Board REFERENCE Figure 84 Optical System 278 1200 Series VWD Service Manual Hardware Information 17 Detector Main Board This board controls all information and activities of all assemblies within the detector module The operator enters parameters changes modes and controls the module through interfaces CAN LAN or RS 232C connected to the user interfaces Depending on the version of the detector there are two main boards available G1314D VWD Main board G1314 65070 requires firmware B 06 20 or above G1314E VWD SL Plus Main board G1314 65080 high data rates requires firmware B 06 20 or above Main Features lamp supply for UV lamp LAN on board CAN for internal 1200 interfacing RS 232 for third part control or service e 1 analog output 0 1 V or 0 100 mV full scale leak sensing flow cell safety switch turns off high voltages fan CompactFlash card slot for data recovery G1314E only identification tag operation for flow cell and UV lamp temperature control for optical unit for stable baseline USB for future use no individual battery 1200 Series VWD Service Manual 279 17 Hardware Information Detector Main Board Functional Diagram of Main Board The schematic below shows the functional diagram of the detector electronics Main Power Supply Core Electronic D2 Lamp Po if Filter Motor Filter Assy D2 D2 2 Tag Lamp Grating Motor Gra
151. r detector under conditions where temperature fluctuations could cause condensation within the detector gt If your detector was shipped in cold weather leave it in its box and allow it to warm up slowly to room temperature to avoid condensation Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the detector may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 34 1200 Series VWD Service Manual Delivery Checklist Installing the Detector 3 Ensure all parts and materials have been delivered with the detector The delivery checklist is shown below Please report missing or damaged parts to your local Agilent Technologies sales and service office Table5 X Variable Wavelength Detector Checklist Description Quantity Variable wavelength detector 1 Power cable 1 Flow cell As ordered User Manual 1 Accessory kit CompactFlash Card 1 part of G1314E VWD SL Plus Detector Accessory Kit Contents
152. r dirty or contaminated flow cell windows When the test is started the gain is set to zero To eliminate effects due to absorbing solvents the test should be done with water in the flow cell The shape of the intensity spectrum is primarily dependent on the lamp grating and diode characteristics Therefore intensity spectra will differ slightly between instruments The figure below shows a typical intensity test spectrum The Intensity Test is available in Agilent Diagnostic Software preferred tool Agilent Instant Pilot G4208A via More Diagnosis VWD Lamp Intensity Test Intensity Test Evaluation The Agilent Diagnostic Software and the Instant Pilot evaluate three values automatically and display the limits for each value the average the minimum and the maximum of all data points and passed or failed for each value 1200 Series VWD Service Manual Instensity Test with Instant Pilot Diagnosis Intensity VWD SL Action Result At 254 nm sample cts measured 4018723 Done a Test Functions for User 9 Intensity Test At 254 nm reference cts measured 3612108 Done Perform intensity test Done Max intens gt 320 000 cts measured 6039917 Passed Min intens gt 6 400 cts measured 25507 Passed Avrg intens gt 160 000 cts measured 884724 Passed Finished Used to determine the performance of the lamps and optics T 15 48 Figure 52 Inste
153. re detectors shipped out with different main boards 1 Main Board with 4 connectors TEMP SENS 2 used for Temperature Sensor 2 Main Board with 1 connector TEMP SENS 2 used for Temperature Sensor 3 Main Board with 2 connectors TEMP SENS 2 used for Temperature Sensor The boards with 2 and more connectors allow the addition of an external temperature sensor part number G1314 65801 sensor with cable Installation of external Temperature Sensor 1 Prepare the Temperature Sensor by surrounding the 2 Route the cable between the flow cell and the plate sensor with ESD tape and fix it to the thumb screw of the flow cell N 150 1200 Series VWD Service Manual Test Functions for Service 10 Diagnostic Signals 3 Route the Temperature Sensor cable within the foam 4 Connect the cable to the TEMP SENS 1 connector closer channel towards the rear of the foam to the rear Xp LE oe Next Steps 5 Close the instrument 6 Install the VWD into the stack 1200 Series VWD Service Manual 151 10 Test Functions for Service Setup of the Agilent ChemStation To evaluate the external temperature signal an Agilent ChemStation A 03 02 SR or later is required A macro tempmon mac is required to activate additional diagnostic signals inclusive external temp sensor heater duty cycle board temperature optical temperature MACRO TempMon mac Version 1 0 created by Wolfgang Albre
154. rint sendmodule nvwd RAWD CSIGMON ADD 14 O AUX Sig 0 print sendmodule nvwd RAWD CSIGMON ADD 15 P AUX Sig 1 print sendmodule nvwd RAWD CSIGMON ADD 16 Q AUX Sig 2 print sendmodule nvwd sigs 0 13 O AUX 0 DutyCycle mAU print sendmodule nvwd sigs 1 1 P AUX 1 Temp Sensor 1 print sendmodule nvwd sigs 2 2 Q AUX 2 Temp Sensor 2 I ChemStation Settings for Online Signals O AUX 1 Heater Duty Cycle 1000 cnts 1 duty cycle gt 100000 P AUX 2 TEMP SENSOR 1 EXTERNAL 1000 cnts 1 deg C gt 10000 Q AUX 3 TEMP SENSOR 2 INTERNAL 1000 cnts 1 deg C gt 10000 print Settings loaded EndMacro 152 1200 Series VWD Service Manual Test Functions for Service 10 The macro should be started from the CS command line macro tempmon mac go The macro will be active as long as the CS is active The macro opens the additional signals in the Online Plot window of the CS and stores them as additional signals in the data file Set up blank runs with 1 2 hours duration This will assure that long term effects become visible If intermittent effects are diagnosed then you might use the macro in combination with the normal application method Evaluation of the Diagnostic Signals The plot below shows an example in a stable environment T y Absorbance Signal C 0 1 mAU Sample Signal 1mAU Reference Signal 1 mAU EM man oo Board Tempera
155. rk cable 1 for other cables see below and section Cable Overview in the Service Manual 1 Lab Monitor amp Diagnostic Software 1 CompactFlash Card provided with the G1314E VWD SL PLus 1 G4208A Agilent ChemStation and or Instant Pilot G4208A with appropriate software Preparations Other 1100 1200 modules must have firmware A 06 1x B06 1x or above Locate bench space Provide power connections Unpack the detector Before adding a G1314D VWD or G1314E VWD SL Plus into an existing system assure that the existing modules have been updated to firmware revision A 06 1x B 6 1x or above and the User Interface has the appropriate software revision that supports the G1314D VWD or G1314E VWD SL Plus If you want to use the data never lost feature then assure that the CompactFlash Card is installed in the rear of the G1314E VWD SL Plus 1200 Series VWD Service Manual 39 3 40 Installing the Detector 1 Note the MAC address of the LAN interface rear of the module under the configuration switch see figure below It s required for LAN Configuration see LAN Configuration on page 45 REMOTE Er CONFIG mal RS 232 o LAN Ea CJ t Ce k TT 6131465080 e part number of board
156. rrectly and is free from contamination cell windows buffers and so on Run the filter motor test to determine if the grating motor assembly is defective Exchange the filter motor assembly Run the grating motor test to determine if the grating assembly is defective If defective replace the optical unit Grating Filter Motor Test Failed The motor test has failed test Test 0 Failed Filter motor Test 1 Failed Grating motor During the motor tests the detector moves the motor to the end position while monitoring the end position sensor If the end position is not found the error message is generated Probable cause Suggested actions 1 Defective motor assembly Run the grating motor test to determine if the grating assembly is defective If defective replace the optical unit 1200 Series VWD Service Manual 117 8 Error Information Wavelength Check Failed The automatic wavelength check after lamp ignition has failed When the lamp is switched on the detector waits 1 minute to warm up the lamp Then a check of the deuterium emission line 656 nm via the reference diode is performed If the emission line is more than 3 nm away from 656 nm the error message is generated Probable cause Suggested actions 1 Calibration incorrect Recalibrate the detector Filter Check Failed The automatic filter check after lamp ignition has failed When the lamp is switched on the detector moves the cutoff filt
157. rvice Manual 85 5 Usingthe Detector Run Recovery G1314E only CAUTION This feature is not supported with ChemStation B 03 02 SR1 It will be implemented with ChemStation B 04 01 The figures used in this chapater are from the G1315C DAD SL The G1314E VWD SL figures will look the same Run Recovery Settings For this recovery mode the CompactFlash Card must be in the detector If the LAN communiaction is interruppted no data is stored Have the CompactFlash Card always inserted 86 The detector supports run buffering which means that an amount of run data uv and ch files is stored in a storage medium CompactFlash Card in the detector until either it is overwritten or the detector undergoes a power cycle If there is a temporary network failure or the PC is not able to constantly take the data the stored data is transferred to the ChemStation automatically when the network connection is restored or the PC can take the data so that no loss of data occurs If there is a permanent network failure the Run Recovery dialog box allows you to restore the stored data to the data directory From there you can copy the files to the directory where the files are corrupted or not complete On very large recovery files it may take a long time to restore it to the Agilent ChemStation A sequence will be stopped in case of a network problem When during recovery an error Method Sequence stopped appears the instrument l
158. s An error will be generated status lamp lights red and the logbook will show an error message 184 1200 Series VWD Service Manual Repair 13 Locate the Main Assemblies Locate the Main Assemblies 6 1 2 11 5 8 4 7 3 10 9 Figure 69 Location of Main Assemblies 1 Main Board 7 Heater Assembly 2 Main Power Supply 8 Heater Interface Board Cable 3 Optical Unit 9 Leak Sensor 4 RFID Tag Reader Cell 10 Leak Handling 5 RFID Tag Reader Lamp 11 Temperature Sensor Holder 6 Fan 1200 Series VWD Service Manual 185 13 Repair Replacing the Temperature Sensor When If the temperature sensor is defective Tools required Screwdriver POZI 1 PT3 Parts required Part number Description 1 G1314 65802 Temperature Sensor Assembly includes board and cable 1 G1314 44016 Temperature Sensor Holder Preparations Turn OFF the detector Disconnect the power cable Disconnect capillaries Remove detector from the system and place it on the working bench Remove the Front Panel the Closure the Metal Cover and the Top Foam see Removing the Top Cover and Top Foam on page 182 Remove the Heater Assembly see Replacing the Heater Assembly on page 195 Remove the optical unit see Removing the Optical Unit on page 201 Disconnect and remove the fan assembly First units have pre released foam parts Therefore a single piece of foam is located between optical unit and front sheet metal right of the flow
159. s Deriving From Deuterium Lamps Problem Description With the low noise of the 1200 Series Detector some deuterium lamps may cause periodic baseline instabilities at the end of lamp life This may only be observed when operating at very sensitive detection levels Lamp instabilities come in many different forms The period may vary from a few seconds to hours 1200 Series VWD Service Manual Problem Verification Test Functions for Service 10 If an 1200 Series VWD shows baseline problems the following steps to diagnose whether the lamp is the cause of the baseline instability 1 Ensure that the detector has been properly warmed up see Overview of Diagnostic Signals on page 142 2 Flush the flow cell with water and start runs without sample injections 3 To remove any influence of the flow system remove the cell windows Do not run flow through the cell when cell parts are removed If the baseline continues to show the problem the lamp should be replaced If the baseline is stable after removing the cell windows look for other possible causes of the problem for example dirty flow cell column sampler pump etc Solution Replace the lamp after problem verification Part Number Description usable in G1314 60101 G1314 60100 2140 0820 2140 0813 5181 1530 2140 0590 long life deuterium lamp with RFID tag long life deuterium lamp without RFID tag long life deuterium lamp with RFID t
160. s VWD Service Manual General Error Messages Error Information 8 General error messages are generic to all Agilent 1200 Series HPLC modules Timeout The timeout threshold was exceeded Probable cause 1 The analysis was completed successfully and the time ut function switched off the pump as requested 2 Anot ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Shutdown Suggested actions Restart the analysis where required Check the logbook for the occurrence and source of a not ready condition An external instrument has generated a shut down signal on the remote line The detector continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in an external instrument with a remote connection to the system 2 Shut down in an external instrument with a remote connection to the system 3 The degasser failed to generate sufficient vacuum for solvent degassing 1200 Series VWD Service Manual Suggested actions Fix the leak in the external instrument before restarting the pump Check external instruments for a shut down condition Check the degasser module for an error condition Refer to the Reference Manual for the Agilent 1200 Series vacuum degasser 109 110 Error Information Remote Timeout A not r
161. s provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent 1100 1200 Series modules The other end depends on the instrument to be connected to Agilent 1100 1200 to 3390 Integrators Connector01046 60203 Pin 3390 Pin Agilent Signal Name Active 1100 1200 TTL 2 1 White Digital ground NC 2 Brown Prepare run Low 7 3 Gray Start Low a NC 4 Blue Shut down Low M NC 5 Pink Not connected NC 6 Yellow Power on High NC 7 Red Ready High NC 8 Green Stop Low NC 9 Black Start request Low 1200 Series VWD Service Manual 265 16 Identifying Cables Agilent 1100 1200 to 3392 3 Integrators Connector01046 60206 Pin 3392 3 Pin Agilent Signal Name Active 1100 1200 TTL 3 1 White Digital ground NC 2 Brown Prepare run Low 11 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 9 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low Agilent 1100 1200 to 3394 Integrators Connector01046 60210 Pin 3394 Pin Agilent Signal Name Active 1100 1200 TTL lt 9 1 White Digital ground NC 2 Brown Prepare run Low 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 5 14 7 Red Ready High 6 8 Green Stop Low 1 9 Black Start request Low 13 15 Not connected 266
162. s the different injector steps to perform all the maintenance routines Agilent Lab Advisor also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor Software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues 106 1200 Series VWD Service Manual 1200 Series VWD Service Manual 8 Error Information What Are Error Messages 108 General Error Messages 109 Detector Error Messages 114 This chapter describes the meaning of detector error messages and provides information on probable causes and suggested actions how to recover from error conditions E Agilent Technologies 107 8 Error Information What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the instrument logbook 108 1200 Serie
163. s to the module is product number Diagnostic Software Interface shows the settings of possible NO access at all to the module Type amp the G1314B Use Recover with Agilent is possible Firmware on Type can be changed via Diagnostic Software Type amp Use Recover with Agilent page 226 command line as described Firmware on page 226 Diagnostic Software Type amp under Recover with Agilent Firmware on page 226 ChemStation on page 227 224 1200 Series VWD Service Manual Table 25 Recover From Wrong Type Repair 13 User Interface incorrect but valid type incorrect but valid type incorrect and invalid type Instant Pilot G4208A Control Module 61323 Agilent Diagnostic Software preferred tool comes up with an error access to the module is possible via Service Mode as described under Recover with Instant Pilot on page 227 comes up with an error NO access to the module is possible Use Recover with Agilent Diagnostic Software Type Only on page 226 shows the incorrect product number access to the module is possible as described in Recover with Agilent Diagnostic Software Type Only on page 226 shows resident module G1314B R NO type change possible Use Recover with Agilent Diagnostic Software Type amp Firmware on page 226 shows resident or unsupported module NO type change possible Use Recover with Agilent Diagnostic Software Type amp Firmware
164. s with internal or external standards To check photometric accuracy of the Agilent 1200 Series VWD it is necessary to have more precise information on path lengths of the VWD flow cells The correct response is expected response correction factor Please find below the details of the Agilent 1200 Series VWD flow cells Table 18 Correction factors for Agilent VWD flow cells G1314D G1314E Flow cell type with RFID tag Cell Part number Path length Path length Correction volume nominal actual factor Standard flow cell 14 ul 61314 60186 10 mm 10 15 0 19 mm 10 10 15 Semi micro flow cell 5 yl G1314 60183 6 mm 6 10 0 19 mm 6 6 10 Micro flow cell 2 pl G1314 60187 3mm 2 80 0 19 mm 3 2 8 High Pressure flow cell 14 ul G1314 60182 10 mm 10 00 0 19 mm 6 5 75 1200 Series VWD Service Manual 97 6 Howto optimize the detector Match the Flow Cell to the Column NOTE However you have to be aware that there are additional tolerance of gasket thickness and its compression ratio which is supposed to be very small in comparison with the machining tolerance 98 1200 Series VWD Service Manual How to optimize the detector 6 Set the Detector Parameters 1 Set peakwidth as close as possible to the width at half height of a narrow peak of interest Refer to Peakwidth Settings on page 91 2 Choose the sample wavelength at a longer wavelength than the cut off wavelength of the mobile phase at a wavelength where
165. sample diode assembly is located on the left side of the optical unit The reference diode assembly is located in the front of the optical unit Photo Diode ADC analog to digital converter The photo diode current is directly converted to digital data direct photo current digitalization The data is transferred to the detector main board The photo diode ADC boards are located close to the photo diodes 16 1200 Series VWD Service Manual Introduction to the Variable Wavelength Detector 1 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal Agilent 1200 Series module data transfer and synchronization One analog output provides signals for integrators or data handling systems The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch See your software documentation for further information e The power input socket accepts a line voltage of 100 240 volts AC 10 with a line frequency of 50 or 60 Hz Maximum power consumption is 220 VA There is no voltage selector on your module because the pow
166. selection is important for optimal performance and lifetime Cell tag for Agilent flow cells with RFID tags If no RFID tag cell is used detector icon will become gray cell tag not ready and analysis is disabled Help online help r UV lamp Tag f Automatic Mode only if RFID Tag is available C Use UV lamp anyway Lamp Type M gt Cell Tag use Cell only if RFID Tag is available use Cell anyway Cancel Help The detector status shows Cell tag in yellow in case the flow Beam cag cell with the RFID tag is not inserted The detector icon is gray and the system is not ready Wavelength 254 nm Figure 42 Detector Configuration Settings If the flow cell temperature is critical for your chromatography or your environment is stable you may set the Temperature Control to off This will lower the optical unit and flow cell temperature by some degree 1200 Series VWD Service Manual 83 5 Using the Detector Special Settings of the Detector Online Spectra 1 To view the online spectra select Online Spectra NOTE This online spectrum is taken during a stop flow condition only while the peak is kept in the flow cell see Scanning with the VWD on page 85 ant 5 Not Ready Information A A B A 100 00 to 1000 0 190 E 400 Figure 43 Online Spectra Window 2 Change the absorbance and wavelength range according your needs 84 1200 Series VWD Serv
167. settings in Using Stored mode 1200 Series VWD Service Manual 61 4 LAN Configuration 6 Change the IP address in this example 134 40 27 99 and type to list current settings cx Telnet 134 40 Yip 134 40 27 99 change of IP setting to gt LAN Status Initialization mode is Using Stored TCP IP Properties active IP Address 134 40 27 104 Subnet Mask 255 255 248 0 i i M Gatouss 134 40 34 4 active TCP IP settings stored Address 134 40 27 99 255 255 248 0 stored TCP IP settings in non volatile memory Gateway 134 400 24 1 TCP IP status here ready no Conner sons connected to PC with controller software here not connected Figure 25 Telnet Change IP settings 7 When you have finished typing the configuration parameters type exit and press Enter to exit with storing parameters cx C WINDOWS system32 cmd exe Agilent Technologies G1314E DE819800802 gt exit Connection to host lost C N Figure 26 Closing the Telnet Session If the Initialization Mode Switch is changed now to Using Stored mode the instrument will take the stored settings when the module is re booted 62 1200 Series VWD Service Manual With the Instant Pilot G4208A LAN Configuration 4 To configure the TCP IP parameters before connecting the detector to the network the Instant Pilot G4208A can be used 1 From the Welcome screen press the More button 2 Select Configure 3 Press the VWD VW
168. sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1200 Series VWD Service Manual 159 11 Maintenance and Repair CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD can damage electronic boards and components 2 In order to prevent damage always use an ESD protection when handling electronic boards and components CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD gt To prevent accidental electrostatic discharge when coming into contact with components inside the instrument touch one of the metal housing panels at the front of the instrument 160 1200 Series VWD Service Manual Maintenance and Repair 11 Cleaning the Detector The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth allowing liquid to drip into the detector WARNING Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an exessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings 1200 Series VWD Service Manual 161 11 Maintenance and Repair Using the ESD Strap 162 Electronic boards are sensitive to electrostatic discharge ESD In order to preve
169. shold Limit Values according to the American Conference of Governmental Industrial Hygienists Table 49 UV Radiation Limits Exposure day Effective Irradiance 8 hours 0 1 uW cm 10 minutes 5 0 W cm Typically the radiation values are much smaller than these limits Table 50 UV Radiation Typical Values Position Effective Irradiance Lamp installed 50 cm distance average 0 016 uW cm Lamp installed 50 cm distance maximum 0 14 uW cm 1200 Series VWD Service Manual 305 18 Appendix Solvent Information 306 Observe the following recommendations on the use of solvents Flow Cell Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell Prevent any crystallization of buffer solutions This will lead into a blokkage damage of the flow cell If the flow cell is transported while temperatures are below 5 degree C it must be assured that the cell is filled with alcohol Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add small of organic solvents e g Acetonitrile or Methanol 5 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide pota
170. ssium chloride and so on High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCl O5 gt 2COCl 2HCI This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol 1200 Series VWD Service Manual Appendix 18 Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF 1200 Series VWD Service Manual 307 18 Appendix Declaration of Conformity for HOX2 Filter Declaration of Conformity for HOX2 Filter 308 Declaration of Conformity We herewith inform you that the used in Agilents absobance detectors listed in the table below meets the requirements of National Institute of Holmium Oxid
171. t Settings A forced cold start can be used to bring the module into a defined mode with default parameter settings Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnose and repair log books which will not be erased If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 46 Forced Cold Start Settings Bou Dv eger psp ce To return to normal operation set switches back to your default configuration settings 1200 Series VWD Service Manual Hardware Information Main Power Supply Assembly Standard 17 The main power supply comprises a closed assembly and must not be disassembled further for safety reasons In case of a defect the entire power supply needs to be replaced The power supply provides all DC voltages used in the module The line voltage can vary in a range from 100 240 volts AC 10 and needs no manual setting The power supply provides all DC voltages used in the module except for the voltages supplied by the lamp power supply to the deuterium and tungsten lamps in the detectors The line voltage can vary in a range from 100 240 volts AC 10 and needs no manual setting line voltage MPS primary circuits 5V switching regulator linear voltage regulators 36V not used 24V 4 5A 5V 3A 15V 3A 15V 3A
172. tector 2 Slide the top cover towards the rear and fix the top cover Screws 1200 Series VWD Service Manual 229 13 Repair Installing the Foam and the Top Cover 3 Replace the cover 4 Reinstall the Leak Interface and Leak Funnel 5 Replace the Front Panel 6 Replace the detector in the system 230 1200 Series VWD Service Manual Assembling the Main Cover Repair 13 Assembling the Main Cover When If cover is broken Tools required None Parts required Partnumber Description 1 5065 9982 Plastics kit includes base top left and right sides The plastics kit contains all parts but it is not assembled NOTE CAUTION Wrong assembled In case you insert the left or right side in the opposite position you may not be able to remove the side from the top part Take care not to mix up left and right side 1 Place the top part on the bench and insert the left and right side into the top part 2 Replace the cover 1200 Series VWD Service Manual 231 13 Repair Assembling the Main Cover 232 1200 Series VWD Service Manual 1200 Series VWD Service Manual 14 Parts and Materials for Maintenance Overview of Maintenance Parts 234 Accessory Kit 235 Standard Flow Cell 10 mm 14 ul 236 Micro Flow Cell 3 mm 2 ul 238 Semi micro Flow Cell 6 mm bul 240 High Pressure Flow Cell 10 mm 14 ul 242 Cuvette Holder 244 Leak Parts 245 This chapter provides in
173. the analytes have strong absorptivity if you want to get the lowest possible detection limit at a wavelength with moderate absorptivity if you work with high concentrations and preferably where the spectrum is flat for better linearity 3 Consider to use time programming to further optimization 1200 Series VWD Service Manual 99 6 Howto optimize the detector Set the Detector Parameters 100 1200 Series VWD Service Manual 1200 Series VWD Service Manual 7 Troubleshooting and Diagnostics Overview of the Detector s Indicators and Test Functions 102 Status Indicators 103 Available Tests versus Interfaces 105 Agilent Diagnostic Software 106 Overview about the troubleshooting and diagnostic features ORE Agilent Technologies 101 7 Troubleshooting and Diagnostics Overview of the Detector s Indicators and Test Functions 102 Status Indicators The detector is provided with two status indicators which indicate the operational state prerun run and error states of the detector The status indicators provide a quick visual check of the operation of the detector Status Indicators on page 103 Error Messages In the event of an electronic mechanical or hydraulic failure the detector generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided by the user interf
174. ting Assy Temp Sensor Bd Optical Unit Figure 85 Functional Diagram of Main Board 280 1200 Series VWD Service Manual Hardware Information 17 Board Layout and Connectors REMOTE top USB RS 232 bottom CAN CAN ANALOG E TEMP SENS 2 CONFIGURATION man Switch reg me fee H TEMP SENS 1 E o MAIN POWER SUPPLY E i HEATER FAN Q o E RFID LAMP E GRATING MOTOR H ADC ADC LEAK rid i i SENSOR FILTER E SAMPLE REFERENCE RFID E t HE fe CELL A MOTOR fe H IH FILTER GRATING Figure 86 Board Layout and Connectors 1200 Series VWD Service Manual 281 17 Hardware Information Detector Main Board Temperature Control The detector is equipped with a temperature control to improve the baseline stability in unstable environments e g strong variation of the temperature due to air condition systems A heater and a temperature sensor assembly have been added and are controlled by the detector main board and its firmware There are no parameters to be set for the temperature control The figures below show the air flow and the typical temperature gradient in the gradient based on ambient temperature the temperature uplift of 3 Kelvin and the regulation around that value Optical Unit T CONST ee sueeede temperature control no active cooling 3K mm em m m m mw m ambient Figure 88 Temperature Control Regulation
175. tion is available in Agilent Diagnostic Software preferred tool Agilent Instant Pilot G4208A via More Diagnosis VWD Calibration 128 1200 Series VWD Service Manual Test Functions for User 9 Wavelength Verification Calibration When to Calibrate the Detector The detector is calibrated at the factory and under normal operating conditions should not require recalibration However it is advisable to recalibrate after maintenance flow cell or lamp after repair of components in the optical unit after exchange of the optical unit or VWM board ataregular interval at least once per year for example prior to an Operational Qualification Performance Verification procedure and when chromatographic results indicate the detector may require recalibration Wavelength Verification Calibration with Instant Pilot Current Dev nm Zero Ord 0 200 actual deviation Ref Line 0 100 history Action Result Wait until UV lamp has warmed up Done Abort Wait until lamp s ON Done Perform zero calibration Done EY Perform reference line calibration Done Perform wavelength compensation Done Cont Measure deviation Figure 54 Wavelength Verification Calibration with Instant Pilot 1200 Series VWD Service Manual 129 9 Test Functions for User Holmium Oxide Test 130 This test verifies the calibration of the detector against the three wavel
176. tions 2 Physical Specifications Table3 Physical Specifications Type Specification Comments Weight 11 kg 25 Ibs Dimensions height x width x depth 140 x 345 x 435 mm 5 5 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 120 VA 100 W 341 BTU Maximum Ambient operating temperature 0 55 C 32 131 F Ambient non operating temperature 40 70 C 4 158 F Humidity lt 95 at 25 40 C 77 104 F Non condensing Operating altitude Up to 2000 m 6500 ft Non operating altitude Up to 4600 m 14950 ft For storing the instrument Safety standards IEC CSA UL EN Installation Category II Pollution Degree 2 For indoor use only 1200 Series VWD Service Manual 27 2 Site Requirements and Specifications Performance Specifications Table 4 X Performance Specifications G1314D VWD and G1314E VWD SL Plus Type Specification Comments Detection type Double beam photometer Light source Deuterium lamp Wavelength range Short term noise Drift Linearity Wavelength accuracy Maximum sampling rate Data Recovery Card DRC Band width 190 600 nm 0 15 x 10 AU at 230 nm 1x 10 AU hr at 230 nm gt 2 5 AU 5 upper limit 1nm 20 Hz 160 Hz Unique data never lost insurance Prevents data losses for example in case of communication breakdown 6 5 nm typical Under specified condtions See Specifi
177. ture 0n m 10 C Optical Temperature PAX perd EI miner oa ee We i APIS AIT T i Heater Duty Cycle 100000 cts MIS AA Une Cure Nen fs p pan Pen OMAN m sur d Parent Pal P pr S External Temperature Wa y 10000 cts m Internal Temperature 0m 10000 cts Figure 62 Example plot from ChemStation The scaling of the Sample and reference Signal is different compared to the Absorbance Signal 1200 Series VWD Service Manual 153 10 Test Functions for Service 154 Depending on the initial duty cycle level the detector is running stable typically at 40 at the environmental conditions 30 minutes after lamp on when the temperature regulation starts to work the regulation is about 2 5 K If the temperature change is more than 2 5 K the heater duty cycle will run against the lower or upper limit and the baseline starts to drift continuously until the environment comes back into the regulation range The next example shows an extreme situation in a lab The detector runs stable the heater duty cycle AUX 1 runs around 40 Then the temperature in the lab changes from 26 C to more than 32 C The heater tries to reduce the heater power but runs at 30 minutes against its lower limit 5 The result is that the temperature in the detector optical and main board increases all the time and therefore the baseline is continuously drifting 1200 Series VWD Service Manual Test Functions for Service 10 Diagnostic Signals
178. upper power limit hit excessive ambient temperature drop means lower power limit hit excessive ambient temperature increase Probable cause Suggested actions 1 Ambient conditions have changed too much Verify that the reproducibility of your results during the run so that optimum results may is not affected not be guaranteed Expose the detector to more stable ambient conditions 1200 Series VWD Service Manual 121 8 Error Information No Run Data Available In Device In a very rare case the capacity of the CompactFlash Card is not sufficient This could happen for example when the interrupt of LAN communication takes longer and the detector uses special settings e g full data rate at 80 Hz plus full spectra plus all signals during data buffering Probable cause Suggested actions 1 CompactFlash Card is full Correct communication problem Reduce data rate 122 1200 Series VWD Service Manual 1200 Series VWD Service Manual 9 Test Functions for User Intensity Test 124 Checking the Photocurrent 126 Wavelength Verification Calibration 128 Holmium Oxide Test 130 This chapter describes the detector s built in test functions Agilent Technologies 123 9 Test Functions for User Intensity Test 124 The intensity test measures the intensity of the deuterium lamp over the full VWD wavelength range 190 600 nm The test can be used to determine the performance of the lamp and to check fo
179. velength solvent temperature and other parameters C is the concentration of the absorbing species usually in g l or mg l d is the path length of the cell used for the measurement Therefore flow cells with longer path lengths yield higher signals Although noise usually increases little with increasing path length there is a gain in signal to noise ratio For example in Figure 50 on page 97 the noise increased by less than 10 but a 70 increase in signal intensity was achieved by increasing the path length from 6 mm to 10 mm When increasing the path length the cell volume usually increases in our example from 5 13 ul Typically this causes more peak dispersion As Figure 50 on page 97 demonstrates this did not affect the resolution in the gradient separation in our example As a rule of thumb the flow cell volume should be about 1 3 of the peak volume at half height To determine the volume of your peaks take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3 1200 Series VWD Service Manual How to optimize the detector 6 Analysis of pesticide standard 6 mm optical path length Absorbance T0 mm optical path length T I la UN M l 1 J y A y T 20 Time min Figure 50 Influence of Cell Path Length on Signal Height Traditionally LC analysis with UV detectors is based on comparing measurement
180. voltage falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective or non Agilent lamp Exchange the lamp 2 Defective power supply Exchange the power supply 3 Defective VWM board Exchange the VWM board 114 1200 Series VWD Service Manual Error Information 8 Lamp Ignition Failed The lamp failed to ignite The processor monitors the lamp current during the ignition cycle If the lamp current does not rise above the lower limit within 2 5 seconds the error message is generated Probable cause Suggested actions 1 Lamp not connected Ensure the lamp is connected 2 Defective or non Agilent lamp Exchange the lamp 3 Defective power supply Exchange the power supply 4 Defective VWM board Exchange the VWM board Heater Current Missing The lamp heater current in the detector is missing During lamp ignition the processor monitors the heater current If the current does not rise above the lower limit within one second the error message is generated Probable cause Suggested actions 1 Lamp not connected Ensure the lamp is connected 2 Ignition started without the top foam in Replace the top foam and turn on the lamp place 3 Defective VWM board Exchange the VWM board 4 Defective or non Agilent lamp Exchange the lamp 5 Defective power supply Exchange the power supply 1200 Series VWD Service Manual 115 8 Error Information Calibration Failed The intensity
181. with RFID tag NO lamp without RFID tag YES via LMD or Instant Pilot The detector provides the following EMF counters e Deuterium Lamp On Time Number of UV lamp ignitions 1200 Series VWD Service Manual 21 1 22 Introduction to the Variable Wavelength Detector Using the EMF Counters The user settable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The useful lamp burn time is dependent on the requirements for the analysis high or low sensitivity analysis wavelength etc therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When instrument performance indicates maintenance is necessary take note of the values displayed by lamp counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled This function is only available via LMD or Instant Pilot 1200 Series VWD Service Manual 1200 Series VWD Service Manual 2 Site Requirements and Specifications Site Requirements 24 Physical Specifications 27 Performa
182. www chem agilent com scripts cag_firmware asp 1200 Series VWD Service Manual Hardware Information 17 Agilent 1100 1200 Series Interfaces The Agilent 1100 1200 Series modules provide the following interfaces Table 42 Agilent 1100 1200 Series Interfaces Module CAN LAN BCD LAN GPIB RS 232 Analog APG Special optional on board Remote Pumps G1310A ISO 2 Yes No Yes Yes 1 Yes G1311A QUAT G1312A BIN G2226A NANO G1312B BIN SL 2 Yes No Yes Yes 1 Yes G1361A PREP 2 Yes No No Yes No Yes CAN DC OUT for CAN slaves Samplers G1313A STD 2 Yes No Yes Yes No Yes G1329A STD 2 Yes No Yes Yes No Yes THERMOSTAT for G1329B STD SL G1330A B G2260A PREP G1364A FRC 2 Yes No Yes Yes No Yes THERMOSTAT for G1367A B C D G1330A B WPS CAN DC OUT for G1377A pWPS CAN slaves G2258A D LOOP Detectors G1314A B VWD 2 Yes No Yes Yes 1 Yes G1314C VWD SL 2 Yes No No Yes 1 Yes G1314D VWD 2 No Yes No Yes 1 Yes G1314E VWD SL 2 No Yes No Yes 1 Yes 1200 Series VWD Service Manual 287 17 Hardware Information Table 42 Agilent 1100 1200 Series Interfaces Module CAN LAN BCD LAN GPIB RS 232 Analog APG Special optional on board Remote G1315A B DAD 2 Yes No Yes Yes 2 Yes G1365A B MWD G1315C DAD SL 2 No Yes No Yes 2 Yes G1365C MWD SL G1315D DAD G1365D MWD G1321A FLD 2 Yes No Yes Yes 1 Yes G1362A RID G4280A ELSD No No NO No Yes Yes Yes EXT Contact AUTOZERO Others G1316A TCC No No No A Yes No Yes G1316B TCC SL
183. y Sample Name isocratic test sample Sample Amount fo ISTD Amount o Comment Isocratic test sample 1 ul 30 70 H20 Acetonitrile 1 5 ml min zi Multiplier Dilution fi Bun Method OK Cancel Sample Information Help 15 Fill the content of an isocratic standard sample ampoule into a vial and seal the vial with a cap and place the vial into autosampler tray position 1 1200 Series VWD Service Manual Using the Detector 5 Running the Sample and Verifying the Results 1 To start a run select the menu item RunControl gt Run Method 2 This will start the 1200 modules and the online plot on the Agilent ChemStation will show the resulting chromatogram DAD1 A Sig 254 4 Ref 500 100 041014410 140003 D mAU yv V NN C EN a rr a a a me mad a a mL a me a yd am er om a min Figure 40 Chromatogram with Isocratic Test Sample Information about using the Data Analysis functions can be obtained from the Using your ChemStation manual supplied with your system 1200 Series VWD Service Manual 81 5 Using the Detector Special Settings of the Detector In this chapter special settings of the G1314D VWD and G1314E VWD SL Plus are described Control Settings Set up WHO Sina x r Lamp gt Enror Method 3 Lamp turn on and off of UV lamp Ha Online Spectra ae At Power On automatic lamp on at power on visi Ready
184. ystem cT 1050 Quatern Pure Selected Modules Instrument Name Lct100 01 Delete Initially Start Instrument Sess C Yes Initial Screen Win Normal C con e 1100 Modular System ok Cancel Device Address C Identify by Host Name Identify by IP Address System 2 Device Configuration Modular 3D LC IP Address fi 34 40 24 230 r Modules m 1100 a GPIB Addre Cancel Help Selected Modules 1100 System 1100 System Access IP 134 40 27 95 QD covet l Figure 29 Changing the TCP IP settings of the Agilent ChemStation 4 1200 Series VWD Service Manual 65 4 LAN Configuration 2 Adda TCP IP connection to communicate with the module Use the IP address of the module If using a corporate LAN IP addresses need to be supplied by the responsible IT department Also the LAN needs to be able to handle additional traffic 3 Savethe configuration close the Configuration Editor and start the Agilent ChemStation 66 1200 Series VWD Service Manual 1200 Series VWD Service Manual 5 Using the Detector Setting up an Analysis 68 Special Settings of the Detector 82 This chapter provides information on how to set up the detector for an analysis and explains the basic settings ORE Agilent Technologies 67 5 Usingthe Detector Setting up an Analysis This chapter can be used for preparing the system tolearn the set up of an HPLC

Agilent 1200 Series Variable Wavelength Detector G1314D /G1314E

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