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Agilent 1200 Series High Performance Autosamplers & Micro Well

1. CAN bus cable Autosampler Thermostat cable AC power Remotecable AC ower Analog signal paw to recorder Figure 11 Recommended Stack Configuration Thermostatted Autosampler Rear View 1 1200 Series Autosamplers Service M anual 41 3 Installing the Autosampler Installing the Autosampler Parts required Description 1 Sampler Power cord Preparations Locate bench space Provide power connections Unpack the sampler WARNING Instruments are partially energized when switched off The power supplies still use some power even if the power switch on the front panel is turned off gt To disconnect the thermostatted autosampler from line power unplug the power cord from the autosampler and the ALS thermostat gt Make sure that it is always possible to access the power plug WARNING Personal injury To avoid personal injury keep fingers away from the needle area during autosampler operation gt Do not attempt to insert or remove a vial or a plate when the needle is positioned CAUTION Defective on arrival problems If there are signs of damage to the autosampler please do not attempt to install the autosampler Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and in
2. Needle carrier 5 i Peristaltic pump Needle Needle seat Injection valve 1200 Series Autosamplers Service M anual 171 8 Maintenance Maintenance Functions Some maintenance procedures require the needle arm metering device and needle carrier to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position For details refer to Maintenance Functions on page 143 172 1200 Series Autosamplers Service M anual Maintenance 8 Early Maintenance Feedback EMF Maintenance requires the exchange of components in the flow path which are subject to mechanical wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user setable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EM F Counters The autosampler provides four EMF counters Each counter increments with autosampler use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has be
3. 46 1200 Series Autosamplers Service M anual Installing the Autosampler 3 10 Remove the tray and the plastic cover from the tray base place the air channel adapter into the sampler tray base Make sure the adapter is fully pressed down This assures that the cold airstream from the thermostat is correctly guided to the tray area of the well plate sampler Air channel adapter Figure 14 Installation of Thermostat and Autosampler 11 Re install the tray 12 Ensure the power switch on the front of the sampler is OFF and the power cables are disconnected 13 Connect the cable between the sampler and the thermostat see Figure 15 on page 48 14 Connect the power cables to the power connectors 15 Connect the CAN cable to other Agilent 1200 series modules 16 If a Agilent ChemStation is the controller connect the LAN connection to the LAN interface 17 Connect the APG remote cable optional for non Agilent 1200 Series instruments 18 Ensure the side panel is correctly installed 19 Turn ON power by pushing the button at the lower left hand side of the sampler 20 Close the front door 1200 Series Autosamplers Service M anual 47 3 Installing the Autosampler The exhaust fan will turn ON and remove the vapor from the tray compartment After 1 2 minutes the sampler will start tile hardware initialisation process At the end of this process the status LED should be off Sampler Thermostat cable CAN bus AC Po
4. 91 5 Using the Autosampler Turn ON and Initialization Steps A successful turn on initialization takes about 3 5 minutes and consists of five steps 1 WPS turn on begins when the main power button is pushed ON Power indicator turns green Front cover latch activates immediately Main fan and exhaust fan turn on immediately 3 Main board self test begins Status indicator tests red green and yellow then goes to yellow This takes about 20 seconds from turn on The status indicator remains yellow until the initialization process is complete The user interface indicates initializing during this period 4 The vapor blowout period begins This lasts for about 2 minutes WPS sample transport and sampling unit initialization begins at the 2 minutes mark from turn on if the front cover is closed If the front cover is open at the 2 minutes mark initialization will start only when the front cover is closed Initialization takes about 1 5 minutes When initialization is complete the needle is in the needle seat the needle lock is down and the status indicator is off StatusLED Power LED Figure 28 Instrument LED indicator 92 1200 Series Autosamplers Service M anual 1200 Series Autosamplers Service M anual e 6 Optimizing Performance Optimizing Performance 94 Optimization for Lowest Carry Over 95 Using the Automated Needle Wash 98 Using the Flush Port 98 Cleaning the needleseat 99 Fast In
5. Choice of Seat Capillary on page 107 49 3 Installing the Autosampler Flow Connections to the Sampler from pump Corrugated tube e _ _ y l Wh elt ie HU e aam nmi jpn Loop capillary waste tube Serial to waste to column Figure 16 Hydraulic Connections 50 1200 Series Autosamplers Service M anual Installing the Autosampler Installing the Sample Tray Press the bottom on the right side to release the front door Lift the front door Load the sample tray with sample well plates and vials as required FP WN re Slide the sample tray into the autosampler so that the rear of the sample tray is seated firmly against the rear of the sample tray area 5 Press the front of the sample tray down to secure the tray in the autosampler If the tray pops out of position the air channel adapter is not correctly inserted 1200 Series Autosamplers Service M anual 3 51 3 Installing the Autosampler 52 1200 Series Autosamplers Service M anual Installing the Autosampler 3 Transporting the Sampler When moving the autosampler inside the laboratory no special precautions are needed However if the autosampler needs to be shipped to another location via carrier ensure e The transport assembly is in the park position Use the Lab Monitor and Diagnostic software or the Instant Pilot for this command e The vial tray and the sample transport
6. Description Part Number LAN Communication Interface Board G1369A G1369 60001 One board is required per Agilent 1200 stack It is recommended to add the LAN board to the detector with highest data rate For the configuration of the G1369A Lan Communication Interface card refer to its documenation The following cards can be used with the Agilent 1200 Series modules 1200 Series Autosamplers Service M anual 69 4 Configuring the Autosampler Table 16 LAN Boards Type Vendor Supported netw orks G1369A Agilent Technologies Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX recommended for 61369 60001 re ordering J 4106A Hewlett Packard Ethernet 802 3 RJ 45 10Base T J 4105A Hewlett Packard Token Ring 802 5 DB9 RJ 45 10Base T J 4100A Hewlett Packard Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX BNC 10Base2 These cards may be no longer orderable M inimum firmware of these Hewlett Packard J etDirect cards is A 05 05 Table17 Recommended LAN cables Cross over network cable shielded 3 m long for point to point connection 5023 0203 Twisted pair network cable shielded 7 m long for hub connections 5023 0202 70 1200 Series Autosamplers Service M anual Configuring the Autosampler 4 Agilent 1100 1200 Series Interfaces The Agilent 1100 1200 Series modules provide the following interfaces Module CAN LAN BCD LAN GPIB RS 232 Analog APG Special optional
7. 6 Replace the plate tray in the tray base 7 Turn ON the sampler close the front door The system will reset automatically 8 In the user interface perform a Sample Transport Self Alignment In the Agilent Lab Monitor amp Diagnostic Software the Sample Transport Self Alignment can be found under the Calibration icon 1200 Series Autosamplers Service M anual 251 9 Repairs Leak Sensor 252 1200 Series Autosamplers Service M anual 1200 Series Autosamplers Service M anual e 10 Parts and Materials for Maintenance Sampler Main Assemblies 254 Vial Trays 257 High Performance Autosampler and SL Version Accessory Kit G1367 68705 259 Micro Well Plate Autosampler Accessory Kit G1377 68705 260 M ulti Draw Kit G1313 68711 Only For G1367B 261 G1373A Injector Purge Kit Parts 262 Thermostat for ALS FC Spotter 264 Ee Agilent Technologies 253 10 Parts and Materials for Maintenance Sampler M ain Assemblies Sampler Main Assemblies 13 1 12 2 3 14 4 15 k s lt A I D AA all i AJ 5 f i 10 j A 6 9 8 7 Figure 31 Autosampler M ain Assemblies Table 47 Autosampler M ain Assemblies Item Description Part Number 1 Ribbon Cable from SU to MTP G1313 81602 2 Sample Transport assembly for G1367B D G1367 60019 Sample Transport assembly for G1377A G1377 60009 254 1200 Series Autosamplers Service M anual Parts and Materials for Maintenance 10 Table 47
8. Configuring the Autosampler One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity Forced Cold Start Settings Switches 1 and 2 do not force storage of this set of parameters in non volatile memory Returning switches 1 and 2 to other positions other than being both up will allow for normal operation Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnose and repair log books which will not be erased If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 27 Forced Cold Start Settings M ode Select 1 3 5 8 rest BOOT i i o o o 0 0 h To return to normal operation set switches back to your GPIB or RS 232C configuration settings Stay Resident Settings Firmware update procedures may require this mode in case of firmware loading errors Switches 1 and 2 do not force storage of this set of parameters in non volatile memory Returning switches 1 and 2 to other positions other than being both up will allow for normal operation If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident part that is it is not operable as a specific module It only uses basic functions of the operating system for example for communication 1200 Series Autosamplers Servic
9. Sample Trays 84 List of Recommended Plates and Closing Mat 85 List of Recommended Vials and Caps 87 Configure Well Plate Types 89 Turn ON and Initialization Steps 92 Optimizing Performance 93 Optimizing Performance 94 Optimization for Lowest Carry Over 95 Fast Injection Cycle and Low Delay Volume 102 Precise Injection Volume 104 Choice of RotorSeal 106 Choice of Seat Capillary 107 Troubleshooting and Diagnostics 109 Agilent Lab Monitor amp Diagnostic Software 110 Overview of the Sampler s Indicators and Test Functions 111 Status Indicators 113 ErrorMessages 115 Maintenance Functions 143 High Performance Autosampler Step Commands 145 Troubleshooting the G1367B D and G1377A Autosampler 148 Errors Which M ay Occur During the Turn ON and Initialization Process 149 Instrument Logbook Errors and Step by Step Repair Process 156 Needle Centering Over the Vialorthe Well 166 Maintenance 167 Introduction to Maintenance and Repair 168 Overview of Main Repair Procedures 171 Maintenance Functions 172 Early Maintenance Feedback EMF 173 Maintenance Procedures 175 Repairs 197 Exchanging Internal Parts 199 1200 Series Autosamplers Service M anual Contents Warnings and Cautions 200 Assembling the Main Cover 202 Light Protection Kit Installation 203 Top Cover and Foam 204 Illumination Assembly 206 Transport Assembly 208 Sampling Unit 211 Analytical Head 214 Peristaltic Pump Motor 216 Injection Val
10. Troubleshooting and Diagnostics 7 3 Metering Sensor Error Probable cause Suggested actions 1 Check the connectors on the SUD board e Check the sampling unit connector on the main board e Check the connector on the sampling unit e Change the analytical head e Change the sampling unit e Change the main board G1367 69520 1200 Series Autosamplers Service M anual 159 7 160 Troubleshooting and Diagnostics 4 Rheodyne Valve Error Probable cause 1 Suggested actions Turn the system off and on twice Check the connectors on the SUD board Check the sampling unit connector on the main board Check the connector on the sampling unit Change the rheodyne valve Change the sampling unit Change the main board G1367 69520 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 5 Needle Lock Error Probable cause Suggested actions 1 e Check the connectors on the SUD board e Check the sampling unit connector on the main board e Check the connector on the sampling unit e Change the sampling unit e Change the main board G1367 69520 1200 Series Autosamplers Service M anual 161 7 Troubleshooting and Diagnostics f 6 Needle Into Seat Error Probable cause Suggested actions 1 Upgrade the firmware revision to A 04 14 or higher and the ChemStation revision to A 08 04 or higher e Check needle position and correct alignment in the pusher e Perform an auto alignment 162 1200
11. gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Your autosampler is delivered with a power cord which matches the wall socket of your particular country or region The plug on the power cord which connects to the rear of the instrument is identical for all types of power cord Electric shock The absence of ground connection and the use of an unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the power cord designed for your region Use of unsupplied cables The use of cables which haven t been supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1200 Series Autosamplers Service M anual 27 2 Site Requirements and Specifications Bench Space The autosampler dimensions and weight see Table 3 on page 29 allow the instrument to be placed on almost any laboratory bench The instrument requires an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inches at the rear for the circulation of air and room for electrical connections Ensure the autosampler is installed in a horizontal position
12. F 3 Gray 3 Gray Start Low i 3 4 Blue 4 Blue Shut down Low ic o6 5 Pink 5 Pink Not connected O 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent 1100 1200 to HP 1090 LC or Signal Distribution Module Table 78 Connector01046 60202 Pin HP 1090 Pin Agilent SignalName Active 1100 1200 TTL 1 1 White Digital ground NC 2 Brown Prepare run Low 4 3 Gray Start Low 7 E 7 4 Blue Shut down Low mie 8 5 Pink Not connected H NC 6 Yellow Power on High 3 7 Red Ready High 6 8 Green Stop Low NC 9 Black Start request Low 1200 Series Autosamplers Service M anual Agilent 1100 1200 to General Purpose Cable Identification 12 Table 79 Connector01046 60201 Pin Universal Pin Agilent SignalName Active 1100 1200 TTL 1 White Digital ground O A oj 2 Brown Prepare run Low gg ne key 3 Gray Start Low o0 Be 4 Blue Shut down Low Ee 5 Pink Not connected 00 am 6 Yellow Power on High 3 O 15 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 1200 Series Autosamplers Service M anual 293 12 Cable Identification BCD Cables Sole as a Lo 10 oe One end of these cables provides a 15 pin BCD connector to be connected to the Agilent 1100 1200 Series modules The other end depends on the instrument to be connected to Agilent 1100 1200 to Gen
13. NC 6 Yellow Power on High NC 7 Red Ready High NC 8 Green Stop Low NC 9 Black Start request Low 1200 Series Autosamplers Service M anual 287 12 Cable Identification Agilent 1100 1200 to 3392 3 Integrators Table 73 Connector01046 60206 Pin 3392 3 Pin Agilent SignalName Active 1100 1200 TTL 3 1 White Digital ground NC 2 Brown Prepare run Low 11 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High _ 9 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 288 1200 Series Autosamplers Service M anual Agilent 1100 1200 to 3394 Integrators Cable Identification 12 Table 74 Connector01046 60210 Pin 3394 Pin Agilent SignalName Active 1100 1200 TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low 5S0 15 E e 3 3 Gray Start Low O NC 4 Blue Shut down Low O 0 NC 5 Pink Not connected le NC 6 Yellow Power on High O 5 14 7 Red Ready High 6 8 Green Stop Low 1 9 Black Start request Low 13 15 Not connected START and STOP are connected via diodes to pin 3 of the 3394 connector 1200 Series Autosamplers Service M anual 289 12 Cable Identification Agilent 1100 1200 to 3396A Integrators Table 75 Connector03394 60600 Pin 3394 Pin Agilent SignalName Active 1100 1200
14. SLS Board 243 Removing the SLS Board 244 Installing the SLS Board 244 Power Supply 246 Removing the Power Supply 247 Installing the Power Supply 248 Leak Sensor 249 Removing the Leak Sensor 250 Installing the Leak Sensor 250 198 1200 Series Autosamplers Service M anual Repairs 9 Exchanging Internal Parts The procedures in this section describe how to exchange defective internal parts You must remove the autosampler from the stack in order to open the main cover 1200 Series Autosamplers Service M anual 199 9 Repairs Warnings and Cautions Open main cover The following procedures require opening the main cover of the autosampler gt Always ensure the autosampler is disconnected from the line power when the main cover is removed gt The security lever at the power input socket prevents the autosampler cover from being taken off when line power is still connected Instrument is partially energized when switched off The power supply still uses some power even if the power switch on the front panel is turned off gt To disconnect the autosampler from line power unplug the power cord Toxic and hazardous solvents The handling of solvents and reagents can hold health risks gt When opening capillary or tube fittings solvents may leak out gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safe
15. Software 110 Agilent on internet 311 311 air circulation 28 algae 309 309 analog cable 282 284 analog signal output 74 APG remote connector 75 ASIC 58 autosampler accessory kit contents 35 259 260 autosampler accessory kit 35 autosampler control 56 autosampler microtiter plate board 58 auxiliary cable 282 296 battery 59 safety information 305 BCD board 66 BCD cable 294 BCD LAN board 66 BCD cable 282 bench space 28 bench space 28 board 312 HP J etDirect card 69 boards interface board BCD LAN 66 LAN card 69 bypass 12 c cable analog 282 284 auxiliary 282 296 BCD 282 294 CAN 297 connecting modules 39 external contacts 283 298 GPIB 283 remote 282 287 CAN cable 297 CAN interface 73 capillaries 49 changing serial number and type 235 235 ChemStation changing serial number and type 236 choice of vials and caps 104 cleaning 170 common electronics 58 compensation sensor open 123 compensation sensor short 124 condensation 28 28 configuration switch default settings 77 description and factory setting 77 Control M odule changing serial number and type 238 D damaged packaging 34 delivery checklist 34 34 draw speed 104 104 DRAW 104 ejectspeed 104 104 EJECT 104 electronics ASIC 58 battery 59 fan drive 59 MTP board 58 sample transport control 59 sampling unit control 60 sensors 57 EMF counters 173 setting 174 using 174 environment 26 28 28 errormessages 111 115 er
16. TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low 5S0 15 E e 3 3 Gray Start Low O NC 4 Blue Shut down Low O 0 NC 5 Pink Not connected le 2 5 NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent 1100 1200 to 3396 Series II 3395A Integrators Use the cable part number 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 290 1200 Series Autosamplers Service M anual Cable Identification 12 Agilent 1100 1200 to 3396 Series III 3395B Integrators Table 76 Connector03396 61010 Pin 33XX Pin Agilent SignalName Active 1100 1200 TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low 5S0 15 E e 3 3 Gray Start Low O NC 4 Blue Shut down Low O 0 NC 5 Pink Not connected le 2 5 NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected 1200 Series Autosamplers Service M anual 291 12 Cable Identification 292 Agilent 1100 1200 to HP 1050 HP 1046A or Agilent 35900 A D Converters Table 77 Connector5061 3378 Pin HP Pin Agilent SignalName Active 1050 1100 1200 TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low
17. Autosampler Main Assemblies Item Description Part Number 3 Sampling Unit assembly for G1367B G1367 60008 Sampling Unit assembly for G1367D G1367 60028 Sampling Unit assembly for G1377A G1377 60008 The assy comes without injection valve and analytical head 4 SLS board not shown G1367 66505 5 Analytical Head assembly 100 ul for G1367B G1367 60003 Analytical Head assembly 40 ul for G1367D G1377 60023 Analytical Head assembly 40 ul for G1377A G1377 60013 6 Injection Valve assembly for G1367B 0101 0921 Injection Valve assembly for G1367D 0101 1422 Micro Injection valve assembly for G1377A 0101 1050 7 Needle Seat assy for G1367B G1367 87101 Needle Seat assy for G1367D without capillary G1367 87105 Seat cap 0 12 mm for G1377 87104 Needle Seat G1367 87303 Seat cap 0 17 mm for G1377 87104 Needle Seat G1367 87302 Needle Seat assy for G1377A without capillary G1377 87101 Seat cap 0 10 mm ID for G1377 87101 Needle Seat G1375 87317 Seat cap 0 075 mm for G1377 87101 Needle Seat G1375 87316 Seat cap 0 05 mml for G1377 87101 Needle Seat G1375 87300 8 Plate Tray base G2258 60011 9 Needle assy for G1367B D G1367 87202 Needle assy for G1377A G1377 87201 10 Needle Carrier assembly G1367 60010 11 Power supply assembly not visible 0950 2528 12 Autosampler M ain Board MTP G1367 66520 Exchange Assembly MTP board G1367 69520 13 Ribbon Cable from ST to MTP G1364 81601 Ribbon Cable from SLS to MTP not visible G1367 81600 14 L
18. G1313 66503 1500 0697 5062 8590 G1367 87300 G1377 87310 G1377 87300 G1375 87315 G1367 60007 5067 1540 G1367 60003 G1377 60023 G1377 60013 5065 4445 G1313 87301 G1375 87312 5061 3356 G1313 87300 G1377 87301 0101 0921 0101 1422 0101 1050 G1367 43200 G1367 47700 267 11 Parts for Repair Table56 Autosampler sampling unit Item Description Part Number 15 Needle Seat assy 150 mm 0 17 mm ID for G1367B G1367 87101 Needle Seat without capillary for G1367D G1367 87104 Needle Seat without capillary for G1377A G1377 87105 Seat capillary 150 mm 0 10 mm ID for G1377 87101 Needle Seat G1375 87317 Seat capillary 150 mm 0 075 mm ID for G1377 87101 Needle Seat G1375 87316 Seat capillary 150 mm 0 05 mm ID for G1377 87101 Needle Seat G1375 87300 Seat capillary 150 mm 0 12 mm ID for G1367 87104 Needle Seat G1367 87303 Seat capillary 150 mm 0 17 mm ID for G1367 87104 Needle Seat G1367 87302 16 Flex board G1313 68715 Air barrier not visible G1367 44105 Stepper motor peristaltic pump not visible 5065 4409 M otor holder not visible G1367 42304 Plate peristaltic pump not visible G1367 44100 268 1200 Series Autosamplers Service M anual Parts forRepair 11 Analytical Head Assembly Table57 Analytical Head Assembly 100 ul for G1367B Item Description Part Number Analytical head assembly 100 ul for G1367B G1367 60003 includes items 1 6 1 Screws 0515 0850 2 Plunger assem
19. Initialization Fails 1 Status indicator remains off but the rest of the turn on initialization process is successful Probable cause Suggested actions 1 The heel of the light pipe is not positioned Correctly position the light pipe correctly above the LED on the main board 2 The main board is defective Replace the main board G1367 69520 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Main Board Initialization Fails Il Status indicator remains off and the rest of the turn on initialization process fails as well Probable cause Suggested actions 1 Refer to the possible causes and actions for step 5 bellow 1200 Series Autosamplers Service M anual 153 Troubleshooting and Diagnostics Problem With the Vapor Blow out Period The vapor blowout period does not end approximately 2 minutes after turn on initialization does not begin Probable cause 1 The front cover is not closed 2 The front cover is closed but the user interface displays a front cover open message 3 The left side cover is removed 4 The left side cover is installed but the user interface displays a side cover open message Suggested actions Close the front cover If the front cover open message is still displayed Make sure the front cover magnet is close enough to the magnet sensor e Check the ribbon cable from the SLS board to the main board e Replace the SLS board G1367 66505
20. Movement 0 Failed X axis e Arm Movement 1 Failed Z axis e Arm Movement 2 Failed Theta needle carrier rotation Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 High friction in transport assembly Exchange the sample transport assembly 3 Defective motor assembly Exchange the sample transport assembly 4 Defective sample transport assembly flex Exchange the sample transport assembly board 5 Defective MTP board Exchange the MTP board 1200 Series Autosamplers Service M anual 129 7 130 Troubleshooting and Diagnostics Valve to Bypass Failed The injection valve failed to switch to the bypass position The switching of the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the bypass position or if the microswitch does not close the error message is generated Probable cause Suggested actions 1 Valve in an intermediate position between Turn the WPS main power OFF and ON the By and the Main position 2 Defective injection valve Exchange the injection valve 3 Defective MTP board Exchange the MTP board 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Valve to M ainpass Failed The injection valve failed to switch to the mainpass position The switching of the injection valve is monitored
21. Removing the Exhaust Fan When Tools required Parts required When defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Partnumber Description 3160 4097 Fan In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 Remove the plate tray from the tray base Remove the transport assembly see Removing the Sample Transport on page 209 Remove the MTP main board see Removing the MTP Main Board on page 233 Remove the exhaust fan Installing the Exhaust Fan 230 Install the exhaust fan Install the MTP main board see Installing the MTP Main Board on page 233 3 Connect the fan connector to the MTP main board Install the sample transport see Installing the Sample Transport on page 209 Install the top cover top plate and foam see Top Cover and Foam on page 204 1200 Series Autosamplers Service M anual Repairs 9 Exhaust Fan 6 Replace the plate tray in the tray base 7 Turn ON the sampler close the front door The system will r
22. Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 6 Needle Into Seat Error during initialization of the Sampler Probable cause Suggested actions 1 1200 Series Autosamplers Service M anual Turn off the system Perform a forced cold start move the 8 bit configuration dip switches 1 2 8 at the rear of the instrument to position 1 Turn on the system Perform an auto alignment Turn off the system Move the 8 bit configuration dip switches 1 2 8 at the rear of the instrument to position 0 Turn on the system Check the connector from needle carrier to the sample transport unit Check the sample transport connectors on the main board sample transport Check the connector on the bottom of the sample transport unit Change the needle carrier assembly 61367 60010 Change the sample transport unit Change the main board G1367 69520 163 7 Troubleshooting and Diagnostics 7 Needle Seat Error Probable cause Suggested actions 1 164 Upgrade the firmware revision to A 04 14 or higher and the ChemStation revision to A 08 04 or higher Check if the needle is installed the sample transport comes without needle Check the needle position and correct alignment in the pusher Check if the seat is not blocked with any parts or material crystals glass Perform an auto alignment Check the connector from the needle carrier to the sample transport unit Check the sample trans
23. The thermostatted sampler dimensions and weight see Table 3 on page 29 allow the instrument to be placed on almost any laboratory bench The instrument requires an additional 25 cm 10 inches of space on either side for the circulation of air and approximately 8 cm 8 1 inches at the rear for electrical connections Ensure the autosampler is installed in a horizontal position If a complete Agilent 1200 Series system is to be installed on the bench make sure that the bench is designed to carry the weight of all the modules For a complete system including the thermostatted sampler it is recommended to position the modules in two stacks see Figure 8 on page 38 Make sure that in this configuration there is 25 cm 10 inches space on either side of the thermostatted sampler for the circulation of air Environment Your autosampler will work within specifications at ambient temperatures and relative humidity as described in Table 3 on page 29 CAUTION Condensation within the autosampler Condensation will damage the system electronics gt Do not store ship or use your autosampler under conditions where temperature fluctuations could cause condensation within the autosampler gt If your autosampler was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 28 1200 Series Autosamplers Service M anual Site Requirements and Specifications 2 Physical Specifications T
24. While in the Tests screen press m m m dot m 10 From the box now displayed select the Command and press Enter 11 Into the box labeled Nester instruction enter the command TYPE XXXXXX where XXXXXX is embedded in single quotes Letters and numbers are created using the up and down arrows XXXXX is the 5 character product number of the module being changed There must be a space between the word TYPE and the product number Some Agilent 1100 1200 series modules require the correct main board version to match the type for example the SL modules 12 Now press the Execute key Below the box a reply line should then say Reply RA 0000 TYPE XXXXX XXXXX is what you just entered 13 Power cycle the module Turn on should be normal In the Records screen the product column should indicate the module you just entered If an other User Interface is also connected start it now Recover Instructions 1 Turn off the module Depending on the module type modules with or without on board LAN the 8 bit Configuration Switch works differently Refer in this case to the module specific manual 2 Change the 8 bit Configuration Switch to Resident see Setting the 8 bit Configuration Switch on page 77 3 Turn the module on 4 Re do the type change using your User Interface and correct the type information Enter the product number without R 5 Turn the module off 6 Change the 8 bit Configuration Switch back to
25. air bubbles may form in the sample plug affecting precision The default draw speed is suitable for the majority of applications however when using viscous samples set the draw speed to lower speed for optimum results A DRAW statement in an injector program also uses the draw speed setting which is configured for the autosampler Eject Speed The default draw speed is suitable for the majority of applications When using large injection volumes setting the eject speed to a higher value speeds up the injection cycle by shortening the time the metering unit requires to eject solvent at the beginning of the injection cycle when the plunger returns to the home position 1200 Series Autosamplers Service M anual Optimizing Performance 6 An EJECT statement in an injector program also uses the eject speed setting which is configured for the autosampler A faster eject speed shortens the time required to run the injector program When using viscous samples a high eject speed should be avoided Table 40 Draw and eject speed Draw speed pl Eject speed pl High performance autosampler Default value 200 200 Minimum 10 10 Maximum 1000 1000 High performance autosampler SL Default value 100 100 Minimum 4 4 Maximum 1000 1000 Micro Well Plate Autosampler with 8 ul loop capillary Default value 4 10 Minimum 0 7 0 7 Maximum 20 100 Micro Well Plate Autosampler with 40 ul loop capillary Default value 4 10 Minimum 0 7 0 7 M
26. and recognized by the operating software from Agilent Technologies Table 18 GPIB Default Addresses Pump 22 DAD MWD 26 FLD 23 Column Compartment 27 VWD 24 Autosampler 28 Agilent 8453A 25 RID 29 RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module next to the GPIB connector 1200 Series Autosamplers Service M anual 73 4 74 Configuring the Autosampler The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table 19 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 2 RX TX 3 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 7 RTS CTS 8 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 24 RS 232 Cable Analog Signal Output The analog signal output e g detector signal or pump pressure signal can be distributed to a recording device For details refer to the description of the main board of the module 1200 Series Autosamplers Service M anual Configuring the Autosampler 4 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to u
27. anual Optimizing Performance 6 Overlapped Injection after gradient is flushed out In this process the injection can be done in parallel to column equilibration phase without compromising any of the Autosampler s specifications This mode has one parameter The time when to start the overlapped injection defined as time after begin of run Considering a composition gradient that ends after 1 min with reestablishing starting conditions the overlap time has to be set to somewhat above 1 min to let the pump fill also the Autosampler s loop with start conditions Overlapped Injection during Run In this process as soon as the sample has reached the column the injection valve is switched back to bypass and the next injection cycle is performed except for switching the injection valve to mainpass This is done after actual run is finished and next analysis is started Doing so the sample preparation time is saved as parallel to the run Switching the valve into the bypass position reduces the system delay volume by the complete Autosampler s flow path volume e g 270ul for G1367B Here the mobile phase is directed to the column without passing sample loop needle and needle seat capillary This can help to have faster cycle times especially if low flow rates have to be used like it is mandatory in narrow bore and micro bore HPLC Having the valve in bypass position can increase the carry over in the system The injection cyc
28. board Installing the SUD Board 1 uF W N Install the new board Ensure the board is positioned between the board guide Secure the board in place with the two screws Reconnect all the connectors to the new board Install the sampling unit see Installing the Sampling Unit on page 213 Install the sample transport see Installing the Sample Transport on page 209 1200 Series Autosamplers Service M anual 241 9 Repairs 6 Install the top cover top plate and foam see Top Cover and Foam on page 204 7 Replace the plate tray in the tray base 8 Turn ON the sampler close the front door The system will reset automatically 9 In the user interface perform a Sample Transport Self Alignment In the Agilent Lab Monitor amp Diagnostic Software the Sample Transport Self Alignment can be found under the Calibration icon 242 1200 Series Autosamplers Service M anual Repairs 9 SLS Board SLS Board 1200 Series Autosamplers Service M anual 243 9 When Repairs Removing the SLS Board Tools required Parts required 244 When defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Partnumber Description G1367 66505 SLS board In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic So
29. by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the mainpass position or if the microswitch does not close the error message is generated Probable cause Suggested actions 1 Valve in an intermediate position between Turn the sampler main power OFF and ON the bypass and mainpass positions 2 Defective injection valve Exchange the injection valve 3 Defective MTP board Exchange the MTP board 1200 Series Autosamplers Service M anual 131 7 132 Troubleshooting and Diagnostics Needle Lock Failed The lock assembly on the sampling unit failed to move successfully The upper and lower positions of the needle lock are monitored by position sensors on the sampling unit flex board The sensors detect the successful completion of the needle lock movement If the needle lock fails to reach the end point or if the sensors fail to recognize the needle lock movement the error message is generated Probable cause Suggested actions 1 Defective or dirty position sensors Clean the position sensor 2 Sticking spindle assembly Exchange the sampling unit assembly 3 Defective needle drive motor Exchange the needle drive motor 4 Defective MTP board Exchange MTP board 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Needle to Needle Seat Position The needle failed to reach the end position in the needle sea
30. detected in another module witha CAN Fix the leak in the external instrument before connection to the system restarting the autosampler 2 Leak detected in an external instrument with Fix the leak in the external instrument before a remote connection to the system restarting the autosampler 3 Shut down in an external instrument with a Check external instruments for a shut down remote connection to the system condition 1200 Series Autosamplers Service M anual 117 7 Troubleshooting and Diagnostics Remote Timeout A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions e g a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause Suggested actions 1 Not ready condition in one of the Ensure the instrument showing the not ready instruments connected to the remote line condition is installed correctly and is set up correctly for analysis 2 Defective remote cable Exchange the remote cable 3 Defective components in the instrument Check the instrument for defects refer to the showing the not ready condition instrument s reference documentation 118 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Synchronization Lost During an analysis
31. error detection and display 0 1 100 ulin 0 1 ul increments Up to 1500 ul with multiple draw hardware modification required Typically lt 0 25 RSD from 5 100 ul Typically lt 1 RSD from 1 5 ul variable volume 400 bar 5880 psi 0 2 5cp 2 x well plates MTP 10 x 2 ml vials 108 x 2 mL vials in2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes 0 5 1 5 2 0mL in 2 x 27 Eppendorf tube plate Also compatible with the Agilent 1200 Series sample capacity extension for further expansion of the sample capacity Typically lt 30s using following standard conditions Default draw speed 200 ul min Default eject speed 200 ul min Injection volume 5 ul Typically lt 0 01 using the following conditions Column 125 x 4mm Hypersil ODS 5 um Mobile phase Water Acetonitrile 80 20 Flow rate 1 ml min Injection volume 1 ul caffeine 1 mg ml 5 ul water to test carryover Outside wash of needle before injection 20 sec with water using flush port 1200 Series Autosamplers Service M anual Table 5 Type GLP features Communications Safety features Injection range Precision Pressure range Sample viscosity range Sample capacity Injection cycle time Carry over Site Requirements and Specifications Performance specifications Agilent 1200 Series High Performance Autosampler SL Specification Early mainten
32. modules require the correct main board version to match the type for example the SL modules Press OK to highlight the complete command Press Done to transfer the information into the main board s memory Press Cancel quit the process Power cycle the module The Maintenance screen should display the correct serial number for this module If an other User Interface is also connected restart the User Interface as well Using the Control Module G1323B 1 2 Connect the control module to the module Turn ON the module On the control module press System F5 then Records F4 Using the up down arrows make sure that the module is highlighted Press FW Update F5 then m This will display a box which says Update Enter Serial Press Enter This will display the box labeled Serial Letters and numbers are created using the up and down arrows Into the box labeled Serial enter the 10 character serial number for the module When the 10 character serial number is entered press Enter to highlight the complete serial number Then press Done F6 Turn the module OFF then ON again The Records screen should display the correct serial number for this module If a Agilent ChemStation is also connected restart the Agilent ChemStation now as well To change the product number go to the System screen 1200 Series Autosamplers Service M anual Repairs 9 8 Press Tests F3 and select the module and press Enter 9
33. new motor and tighten the two holding screws 1200 Series Autosamplers Service M anual 217 9 218 Repairs Take in the new motor and holder Connect the pump motor to the SUD board Re assemble the motor holder and the pump base plate Re install the air channel Assemble the peristaltic pump see Peristaltic Pump on page 195 Install the sampling unit see Installing the Sampling Unit on page 213 oOo N ou FP WwW NY Install the sample transport assembly see Installing the Sample Transport on page 209 9 Install the top cover top plate and foam see Top Cover and Foam on page 204 10 Replace the plate tray in the tray base 11 Turn ON the sampler close the front door The system will reset automatically 12 In the user interface perform a Sample Transport Self Alignment In the Agilent Lab Monitor amp Diagnostic Software the Sample Transport Self Alignment can be found under the Calibration icon 1200 Series Autosamplers Service M anual Repairs 9 Injection Valve Assembly 1200 Series Autosamplers Service M anual 219 9 When Repairs Removing the Injection Valve Assembly Tools required Parts required 220 Pr ul O On OD When defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Partnumber Description 0101 0921 Injection Valve assemb
34. or plates are used see or see List of Recommended Vials and Caps on page 87 V Make sure the needle is correctly installed It should be pushed into the needle carrier as far forward as possible and centered in the vial pusher V Upgrade the firmware revision to A 04 14 or higher and the ChemStation revision to A 08 04 or higher Y Perform an auto alignment without any plates in place V Change the tray G2258 60011 see service note G1367 007 1200 Series Autosamplers Service M anual 8 1200 Series Autosamplers Service M anual 8 Maintenance Maintenance Introduction to Maintenance and Repair 168 Simple Repairs Maintenance 168 Exchanging Internal Parts Repairs Warnings and Cautions 168 Using the ESD Strap 169 Cleaning the module 170 Overview of M ain Repair Procedures Maintenance Functions 172 Early Maintenance Feedback EM F EMF Counters 173 Using the EMF Counters Setting the EMF Limits 174 Maintenance Procedures 175 Removing the Needle Assembly 176 Installing the Needle Assembly 178 Removing the Needle Carrier Assembly 180 Installing the Needle Carrier Assembly 181 Exchange the Needle Seat Assembly G1367 87101 on the G1367B Samplers 182 Exchange the Needle Seat G1367 87105 on the G1367D 168 174 173 174 Exchange the Needle Seat G1377 87101 on the G1377A 183 Exchange the Seat Capillary on the G1377A andG1367D 185 StatorFace 186 RotorSeal 187 Metering Seal and Plunger 188
35. replace the main board G1367 69520 Install the side door If the side door open message is still displayed e Make sure the side cover magnet is present in the side door Make sure the side cover magnet is oriented with the correct pole toward the magnet sensor on the main board and positioned in the side cover such that it is close enough to the magnet sensor e Replace the main board G1367 69520 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Initialization Fails The initialization fails to complete its required movements resulting in one or more of a variety of possible error messages The error message produced depends on when the failure occurred during the initialization Gather the revisions information user interface error and logbook information and error code information as described on page 1 Also consider anything which may have been done just before the initialization failure occurred To date we have seen initialization errors occur for a variety of reasons As an overall troubleshooting plan follow the suggested actions described bellow If this does not help take the information from the instrument logbook refer to the appropriate section in the next chapter and follow the recommended actions step by step Probable cause Suggested actions 1 e Turn the sampler off manually change the X position of the sample transport and rotate the needle carrier to a differe
36. sensitivity resolution precision and lowest carry over Features Increased pressure range up to 600 bar enabling the use of today s column technology sub two micron columns with the Agilent 1200 Rapid Resolution LC System Increased robustness by optimized new parts high speed with lowest carry over by flow through design increased sample injection speed for high sample throughput increased productivity by using overlapped injection mode and flexible and convenient sample handling with different types of sample containers such as vials and well plates Using 384 well plates allows you to process up to 768 samples unattended 8 1200 Series Autosamplers Service M anual Introduction 1 The G1377A Agilent 1200 Series Micro Well Plate Autosampler is designed to perform capillary LC with injection of sample volumes ranging from nL to uL Features A micro Rheodyne valve and the optimized design of the needle seat loop and seat capillaries minimize dispersion A high resolution metering device offers resolution ten times better than a standard autosampler bypass operation facilitates low delay volume increased sample injection speed for high sample throughput flexible and convenient sample handling with different types of sample containers Using 384 well plates allows to process up to 768 samples unattended Technical Principle The well plate sampler transport mechanism uses an X Z theta robot to optimize the positioning of the
37. the 9 64 inch wrench 4 Remove the stator head and stator face 5 Place the new stator face 0100 1851 on the stator head The pins on the stator face must engage in the holes on the stator head Install this stator head face assy on the injection valve Tighten the screws alternately with the 9 64 inch wrench until the stator head is secure Reconnect all the capillaries to the injection valve ports with the 1 4 inch wrench Close the front cover 1200 Series Autosamplers Service M anual Maintenance 8 Rotor Seal When Tools required Parts required 1 1 1 1 1 When poor injection volume reproducibility When leaking injection valve 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 9 64 inch 15 cm long T handle hex key 8710 2394 supplied in accessory kit Partnumber Description 0100 1853 Vespel Rotor Seal for 0101 0921 injection valve G1367B 0100 1849 Tefzel Rotor Seal for 0101 0921 injection valve G1367B 0100 2231 PEEK Rotor Seal for 0101 0921 injection valve G1367B 0100 2088 Vespel Rotor Seal for 0101 1050 injection valve G1377A 0101 1416 PEEK Rotor Seal for 0101 1422 injection valve G1367D CAUTION Damage of the stator face The stator face is held in place by the stator head When removing the stator head the stator face can be damaged gt When removing the stator head ensure the stator face does not fall out of the valve The injection valve 0101 1050 for th
38. the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system 1200 Series Autosamplers Service M anual 119 7 120 Troubleshooting and Diagnostics Leak A leak was detected in the autosampler The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the MTP board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 4 5 6 7 8 9 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Leak Sensor Open The leak sensor in the autosampler has fa
39. the spring draws sample from the vial To reduce potential user mistakes different versions of analytical heads are recognized by RF tags sitting on the exchangeable assembly Table 1 Analytical head Technical Data Standard 100 pl High Pressure 40pl Micro 40 pl G1367 60003 G1377 60023 G1377 60013 Number of steps 15000 15000 60000 Volume resolution 14 nl motor step 5 6 nl motor step 1 4 nl motor step Maximum stroke 100 ul 40 ul 40 ul Pressure limit 400 bars 600 bars 400 bars Plunger material Sapphire Sapphire Sapphire Injection Valve A high pressure 6 port 2 position valve to direct streams of mobile phase and sample to different directions e g via loop to column or directly to column The two position 6 port injection valve is driven by a stepper motor Only five of the six ports are used port 3 is not used A lever slider mechanism transfers the movement of the stepper motor to the injection valve Two microswitches monitor switching of the valve bypass and mainpass end positions The injection valve has a ceramic stator Vespel rotor seal Tefzel 1200 Series Autosamplers Service M anual Introduction 1 seal available and stainless steel head Three screws hold the head and internal components in place No valve adjustments are required after replacing internal components Table 2 Injection Valve Technical Data Standard 0101 0921 Micro 0101 1050 High pressure 0101 1422 Motor type 4V
40. this stage all major performance influencing hardware is flushed internally by the solvent flow For standard applications no additional flushing procedure is required 1200 Series Autosamplers Service M anual Introduction 1 Sampling Unit Sampling Unit The sampling unit consists of subsystems as well The main carrier part is a die casting part which carries the following functional elements Needle drive a Z f a 7 P H A HA A Needle lock he oS 7 4 Gj Analytical head Wz C Flush port Peristaltic pump Needle seat Injection valve Figure5 Sampling unit 1200 Series Autosamplers Service M anual 15 1 16 Introduction Analytical Head The analytical head is driven by the stepper motor connected to the drive shaft by a toothed belt The drive nut on the spindle converts the circular movement of the spindle to linear motion The drive nut pushes the sapphire plunger against the tension of the spring into the analytical head The base of the plunger sits on the large bearing of the drive nut which ensures the plunger is always centered A ceramic ring guides the movement of the plunger in the analytical head The home position of the plunger is sensed by an infra red sensor on the sampling unit flex board while the sample volume is determined by counting the number of steps from the home position 7 nl motor step The backward movement of the plunger driven by
41. to be changed multiple usage boards Use the information from the serial number plate of your module Keep in mind that e the changes become active after a power cycle of the module e the information from the serial number plate of the module is used e the exact type product number is used If the type product number was entered incorrectly the module may become unusable Proceed to With firmware A 06 02 B 01 02 and above a wrong type cannot be entered The entry is checked against the board revision Using the LMD Software Use LMD Software version A 02 02 or later The LMD Software must be configured in Service Mode to have access to the function Board Check and Change Close other user interfaces 1 Start the LMD software 2 Select Tools on the left navigation panel 3 Select in the Tools Selection box Board Check and Change and press the button Start 4 Change the field Type and or Serial as required 1200 Series Autosamplers Service M anual 235 9 Repairs Some Agilent 1100 1200 series modules require the correct main board version to match the type for example the SL modules 6 7 8 Press the button Apply to complete the action A message The type was changed to XXXXXX Close this application and switch off and on the changed LC module Close the LMD Software Power cycle the module Restart the User Interface Using the Agilent ChemStation Module serial numbers are entered by t
42. unit at port 1 and flows directly to the column through port 6 Figure3 Bypass Position The standard injection starts with draw sample from vial In order to do this the needle moves to the desired sample vial position and is lowered into the sample liquid in the vial to allow the metering device to draw up the desired volume by moving its plunger back a certain distance The needle is then raised again and moved onto the seat to close the sample loop In case of an injector program several steps are interspersed at this point 1200 Series Autosamplers Service M anual 13 1 14 Introduction Figure4 Drawing the Sample Flush the Needle Before injection and to reduce the carry over for very sensitive analysis the outside of the needle can be washed in a flush port located behind the injector port on the sampling unit As soon the needle is on the flush port a peristaltic pump delivers some solvent during a defined time to clean the outside of the needle At the end of this process the needle returns to the injection port Inject and Run The final step is the inject and run step The six port valve is switched to the main pass position and directs the flow back through the sample loop which now contains a certain amount of sample The solvent flow transports the sample onto the column and separation begins This is the beginning of a run within an analysis In
43. upper position M ove the needle arm to the vial location on the plate Lowers the needle into the vial Metering device draws the defined injection volume Lifts the needle out of the vial Lowers the needle arm into the seat Command also switches the valve to bypass if it is not already in that position Command lifts the needle and lowers the needle into sample Command can be done more than once maximum draw volume of 40 100 5000 ul cannot be exceeded Use Plunger Home to reset the metering device 145 7 146 Troubleshooting and Diagnostics Table 42 Step Commands Step Action Comments Valve Mainpass Switches the injection valve to the mainpass position Needle Up Mainpass Lifts the needle arm to the upper position and Switches the injection valve to th e mainpass position Troubleshooting If the autosampler is unable to perform a specific step due to a hardware failure an error message is generated You can use the step commands to perform an injection sequence and observe how the autosampler responds to each command Table 43 on page 146 summarizes the step commands and lists the error messages and probable causes associated with each possible failure Table 43 Step Failures Step Function Probable Failure M odes Valve Bypass Valve not connected Defective injection valve Plunger Home Defective or dirty sensor on the sampling unit flex board Defective meter
44. with or without optional interface board Modules with LAN on board PRINT SENDM ODULE Lxxx TYPE XXXXX PRINT SENDM ODULES N xxx TYPE XXXXX module identifier L module identifier N product number XXXXX embedded in single quotes product number XXXXX embedded in single quotes PMP ALS THM TCC VWD DAD MWD FLD RID DAD MWD VWD Some Agilent 1100 1200 series modules require the correct main board version to match the type for example the SL modules 3 Power cycle the module Then restart the Agilent ChemStation If the serial number you have just entered is different than the original module serial number you will be given the opportunity to edit the configure 1200 access screen during the restart of the Agilent ChemStation 4 After restart the serial number type you have just entered can be seen under the Instrument menu of the main user interface screen Using the Instant Pilot G4208A 1 Connect the Instant Pilot to the module Turn ON the module 2 On the Instant Pilot s Welcome screen press More then select Maintenance Using the up down arrows select the module where you have to change the product number or serial number 1200 Series Autosamplers Service M anual 237 9 238 Repairs Press PN SN This will display a screen where you can enter the product number and or serial number Make your changes using the information from the product label of your module Some Agilent 1100 1200 series
45. 1 Hex key 4 0 mm 15 cm long T handle 1 8710 2392 Hex key 9 64 inch 15 cm long T handle 1 8710 2394 Hex key 2 0 mm 1 8710 2438 ESD wrist strap 1 9300 1408 Air channel adapter 1 G1329 43200 Capillary WPS to column 250 mm 0 17 mm ID 1 G1367 87304 WPS leak kit 1 G1367 60006 Tool for micro seat capillary 1 G1377 44900 1200 Series Autosamplers Service M anual 259 10 Parts and Materials for Maintenance Micro Well Plate Autosampler Accessory Kit G1377 68705 260 Table 52 Micro Well Plate Autosampler Accessory Kit Contents G1377 68705 Description Quantity Part Number 96 well plate 0 5 ml PP pack of 10 1 5042 1386 Tubing assembly 1 5063 6527 Filter kit 1 5064 8240 CAN cable 1m 1 5181 1519 Vials screw cap 100 pk 1 5182 0716 Blue screw caps 100 pk 1 5182 0717 Valve catalog 1 5988 2999 Hex key 9 64 inch for injection valve screws 1 8710 0060 Wrenches 1 4 5 16 inch 2 8710 0510 Wrench 4 0 mm open end 1 8710 1534 Rheotool socket wrench 1 4 inch 1 8710 2391 Hex key 4 0 mm 15 cm long T handle 1 8710 2392 Hex key 9 64 inch 15 cm long T handle 1 8710 2394 Hex key 2 5 mm 15 cm long straight handle 1 8710 2412 Hex key 2 0 mm 1 8710 2438 Torque adapter 1 G1315 45003 Air channel adapter 1 G1329 43200 Capillary sampler column 500 mm 0 05 mm ID 1 G1375 87304 40 ul Loop capillary 1 G1377 87300 WPS leak kit 1 G1367 60006 Seat capillary 150 mm 0 075 mm ID 1 G1367 87316 Tool for micro seat capillary 1 G1377
46. 1 02 or to a A Xxx xx Some 1200 series SL modules like the G1312B G1314C G1316B can be downgraded to lower versions by converting the module into a lower version for example a G1312B SL pump is converted to a G1312A and looses the features of the G1312B All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web e http www chem agilent com scripts cag_firmware asp 1200 Series Autosamplers Service M anual 4 65 4 Configuring the Autosampler Optional Interface Boards 66 The Agilent 1200 Series modules have one optional board slot that allows addition of an interface board to the modules Table 14 Optional Interface Boards Description Part Number BCD Board G1351 68701 Fuse 250 mA four are on the board 2110 0004 LAN Communication Interface Board G1369A or G1369 60001 BCD Board The BCD board provides a BCD output for the bottle number of the Agilent 1200 Series autosampler and four external contacts The external contact closure contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused 1200 Series Autosamplers Service M anual Configuring the Autosampler 4 Optional Inter Pos P2 A1 Pos P2 B1 S Vial 10 Plate 2 Pos P2 P24 Vial 984 Pos P1 A1 Pos P1 B1 S Plate 1 Pos P1 H12 Vial 296 Vial 1 Figure22 BC
47. 1 2 A stepper motor 4V 1 2 A stepper motor 4V 1 2 A stepper motor Seal material Vespel or Tefzel Vespel PEEK Stator material Ceramic PEEK Head coated SST Ultralife Number of ports 6 6 6 Switching time lt 150 ms lt 150 ms lt 150 ms Needle Flush Station A needle flush station to wash the outer surface of the injection needle and a peristaltic pump to deliver fresh solvent to the wash station The reservoir for the solvent is located in the solvent cabinet the waste is channeled by a separate flex tube to a waste bottle Needle Lock A needle lock is used to support the needle carrier in its function making a firm seal of the needle in its seat The needle lock arm is driven by a stepper motor connected to the spindle assembly by a toothed belt 1200 Series Autosamplers Service M anual 17 1 Introduction Needle Sample Transport Assembly Needle Sample Transport Assembly Theta axis a B _ my e jna X y A A 4 Xaxis Z axis AN S A O k j j NOD a N PX Needle assembly Needle carrier Reflective light switches Figure6 Needle Sample Transport Assembly The needle sample transport is a multifunctional module capable of moving the needle into various positions such as different wells in two different plates different vials needle wash position and the needle seat position The active movable axes are the X axis the Z axis and the theta axis the 18 1200 Seri
48. 1 3356 2 Leak plane G1313 44501 3 Leak tubing 120 mm 5062 2463 4 Leak funnel 5041 8388 1 reorder gives 5m Figure 41 1200 Series Autosamplers Service M anual Leak System Parts 279 11 Parts for Repair Leak System Parts 280 1200 Series Autosamplers Service M anual 1200 Series Autosamplers Service M anual e 12 Cable Identification Cable Overview 282 Analog Cables 284 Remote Cables 287 BCD Cables 294 Auxiliary Cable 296 CAN Cable 297 External Contact Cable RS 232 Cable Kit 299 LAN Cables 300 298 ee Agilent Technologies 281 12 Cable Identification Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EM C regulations Type Description Part Number Analog cables 3390 2 3 integrators 01040 60101 3394 6 integrators 35900 60750 Agilent 35900A A D converter 35900 60750 General purpose spade lugs 01046 60105 Remotecables 3390 integrator 01046 60203 3392 3 integrators 01046 60206 3394 integrator 01046 60210 3396A Series integrator 03394 60600 3396 Series Il 3395A integrator see details in section 3396 Series III 3395B integrator 03396 61010 HP 1050 modules HP 1046A FLD 5061 3378 HP 1046A FLD 5061 3378 Agilent 35900A A D converter 5061 3378 HP 1040 diode array detector 01046 60202 HP 1090 liquid chromatographs 01046 60202 Signal distribution module 01046 60202 BCD cables 3396 in
49. 12 14 3 300 No Agilent PN 96GreinerV 8 12 14 3 250 No Agilent PN 96N unc 8 12 14 3 400 No Agilent PN Closing mat for all 96 8 12 5042 1389 50 Agilent plates Table32 Recommended Vial plates Description Part Number e Vial plate for 54 x 2 ml vials 6 pk G2255 68700 e Vial plate for 15 x 6 ml vials 1 pk 5022 6539 e Vial Plate for 27 Eppendorf tubes 5022 6538 86 1200 Series Autosamplers Service M anual Using the Autosampler 5 List of Recommended Vials and Caps Table 33 Crimp Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5181 3375 5183 4491 Clear glass 2 5182 0543 5183 4492 5183 4494 write on spot Amber glass 2 5182 3376 5183 4493 5183 4495 write on spot Table 34 SnapTop Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5182 0544 5183 4504 5183 4507 Clear glass 2 5182 0546 5183 4505 5183 4508 write on spot Amber glass 2 5182 0545 5183 4506 5183 4509 write on spot Table 35 Screw Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5182 0714 5183 2067 5183 2070 Clear glass 2 5182 0715 5183 2068 5183 2071 write on spot Amber glass 2 5182 0716 5183 2069 5183 2072 write on spot 1200 Series Autosamplers Service M anual 87 Using the Autosampler Table 36 Crimp Caps Description Septa 100 Pack Silver aluminum Clear PTFE re
50. 2 2 Screw cover 5022 2112 3 Sampler sheet metal kit G1367 68701 Figure 37 Sheet Metal Kit 274 1200 Series Autosamplers Service M anual Parts forRepair 11 Cover Parts Table 64 Covers Item Description Part Number 1 Cabinet kit includes base side panels top and front cover 5067 1556 Cabinet kit includes base side panels top and front cover 5067 1533 When injector purge kit is installed Name plate for Agilent 1200 Series 5042 8901 Light protection kit includes dark front cover and side window 5067 1552 Figure 38 Cover Parts 1200 Series Autosamplers Service M anual 275 11 Parts for Repair Foam Parts 276 Table 65 Foam Parts Item Description Part Number Foam kit includes items 2 and 3 5064 8248 1 Board guides 5041 8395 2 Top foam Order foam kit 3 Bottom foam Order foam kit 1200 Series Autosamplers Service M anual Foam Parts Figure 39 277 1200 Series Autosamplers Service M anual 11 Parts for Repair Power and Status Light Pipes Table 66 Power and Status Light Pipes Item Description Part Number 1 Light pipe power switch 5041 8382 2 Power switch button 5041 8381 3 Light pipe status lamp 5041 8384 4 Power switch coupler 5041 8383 4 D 1 Figure 40 Power and Status Light Pipes 278 1200 Series Autosamplers Service M anual Parts forRepair 11 Leak System Parts Table 67 Leak System Parts Item Description Part Number 1 Leak sensor 506
51. 384N unc 16 24 14 4 80 No Agilent PN 96Agilent 8 12 14 3 400 5042 1386 10 5042 1385 120 96Agilent conical 8 12 17 3 150 5042 8502 25 96CappedAgilent 8 12 47 1 300 5065 4402 1 96Corning 8 12 14 3 300 No Agilent PN 96CorningV 8 12 14 3 300 No Agilent PN 96DeepAgilent31mm 8 12 31 5 1000 5042 6454 50 96DeepN unc31mm 8 12 31 5 1000 No Agilent PN 96DeepRitter41mm 8 12 41 2 800 No Agilent PN 96Greiner 8 12 14 3 300 No Agilent PN 96GreinerV 8 12 14 3 250 No Agilent PN 96N unc 8 12 14 3 400 No Agilent PN Closing mat for all 96 8 12 5042 1389 50 Agilent plates Table 50 Recommended Vial plates Description Part Number Vial plate for 54x2ml vials 6 pk G2255 68700 Vial plate for 15x6ml vials 1 pk 5022 6539 Vial plate for 27 Eppendorf tubes 1 pk 5022 6538 258 1200 Series Autosamplers Service M anual Parts and Materials for Maintenance 10 High Performance Autosampler and SL Version Accessory Kit G1367 68705 Table51 High Performance Autosampler and SL version Accessory Kit G1367 68705 Description Quantity Part Number Capillary sampler column 380 mm 0 17 mm ID 1 01090 87306 96 well plate 0 5 ml PP pack of 10 1 5042 1386 Tubing assembly 1 5063 6527 Filter kit 1 5064 8240 CAN cable 1m 1 5181 1519 Vials screw cap 100 pk 1 5182 0716 Blue screw caps 100 pk 1 5182 0717 Hex key 9 64 inch for injection valve screws 1 8710 0060 Wrench 4 mm both ends 2 8710 1534 Rheotool socket wrench 1 4 inch 1 8710 239
52. 44 The sample transport alignment with the sampling unit and the well plate tray is required to compensate for larger deviations in positioning the needle carrier This function is in the diagnose screen of the Chemstation or the Control Module In the Agilent Lab Monitor amp Diagnostic Software this function is in the Calibration icon The sample transport self alignment is required after disassembling the system or when you exchange e The sample transport e The sampling unit e The MTP main board e The autosampler tray base 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 High Performance Autosampler Step Commands Each movement of the sampling sequence can be done under manual control This is useful during troubleshooting where close observation of each of the sampling steps is required to confirm a specific failure mode or verify successful completion of a repair Each injector step command actually consists of a series of individual commands that move the autosampler components to predefined positions enabling the specific step to be done Table 42 Step Step Commands Action Comments Valve Bypass Plunger Home Needle Up Move to Location Needle into sample Draw Needle Up Needle into Seat 1200 Series Autosamplers Service M anual Switches injection valve to the bypass position M oves the plunger to the home position Lifts the needle arm to the
53. 44900 1200 Series Autosamplers Service M anual Parts and Materials for Maintenance 10 M ulti Draw Kit G1313 68711 Only For G1367B Table 53 Multi Draw Kit Item Description Part Number 1 Seat capillary 500 ul 0 5 mm id G1313 87307 2 Seat capillary 1500 ul 0 9 mm id G1313 87308 3 Union 0100 0900 1200 Series Autosamplers Service M anual 261 10 Parts and Materials for Maintenance G1373A Injector Purge Kit Parts 262 Table 54 Injector Purge Kit Parts for G1367B Autosamplers Description Part Number Plastic cover cabinet kit with rail includes top and side 5064 1533 covers and the bolt carrier for mounting the front door 6 Position 7 Ports 1200 Series CAN valve Assay Reorder G1156 60001 number for repairs only Stator for G1156A valve 0101 1410 1 Groove Rotor seal for G1156A valve 0101 1411 Wellplate Autosampler Purge Accessory kit not orderable G1373 68705 separately includes items 6 22 2x Solvent bottle clear glass 1I 9301 1420 2x Bottle head assembly G1311 60003 Tubing Set 100 cm 2x for solvent connection from degasser G1373 67300 to the purge valve 2x PEEK Adapter for tubing from degasser to valve port 0100 1847 Blanking Nut SST 01080 83202 Flexible Capillary 0 25 mm ID length 320 mm 5065 9980 1 16 front ferrules for SST capillaries 1 16 back ferrules 5065 4454 for SST capillaries Fitting Screw long pack of 10 3 groove Rotor seal for max 600 bar G1367D WPS in
54. 7 87201 Attach the 5 16 inch wrench to hold position at the needle assembly Use the 4 mm wrench to tighten the fitting of the loop capillary Do not hold the needle during this step to avoid to bending it Push the loop capillary into the loop capillary protection tube until it comes out on the sampling unit side Tighten the loop capillary fitting to the analytical head Install the loop capillary corrugated waste tube over the loop capillary Pinch the holder clamp and reinsert the needle assembly into the needle carrier Lock the needle tighter lock system 1200 Series Autosamplers Service M anual Maintenance 8 9 Push the black chain into the needle assy until the stop 10 Check the alignment of the needle in the needle pusher of the needle carrier by viewing from several directions to see that it is aligned in the center of the needle pusher The needle must be centered in the needle pusher as all alignment by the autosampler is calculated from the needle pusher position 11 Remove the silicon safety tube from the needle 12 Replace the plate tray in the tray base Re install the side door and close the front door 13 In the user interface close the Change Needle Seat function and exit the maintenance mode In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found in the Tools icon 1200 Series Autosamplers Service M anual 179 8 Maintenance Removing the Need
55. Agilent 1200 Series High Performance Autosamplers amp Micro Well Plate Autosampler Service M anual Notices Agilent Technologies Inc 2006 2008 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61367 90110 Edition 05 08 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Research Use Only Not for use in Diagnostic Procedures Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these te
56. D 24 00011000 Agilent 8453A 25 00011101 DAD MWD 26 00011010 Column compartment 27 00011011 Autosampler 28 00011100 RID 29 00011101 where 0 means that the switch is down and 1 means that the switch is up RS 232C Communication Settings The communication protocol used in this instrument supports only hardware handshake CTS RTS Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory 1200 Series Autosamplers Service M anual Configuring the Autosampler 4 Table 23 Communication Settings for RS 232C Communication Mode Select 1 2 B 5 6 7 8 RS 232C 1 Baud rate Data Parity Bits Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Table 24 Baud Rate Settings Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 114400 0 1 0 2400 1 1 0 19200 0 1 l 4800 1 1 1 B8400 Table25 Data Bit Settings Switch 6 Data Word Size 0 7 Bit Communication h 8 Bit Communication Table 26 Parity Settings Switches Parity 7 8 0 0 No Parity 1 0 Odd Parity 1 1 Even Parity 1200 Series Autosamplers Service M anual 79 4 80
57. D output for the well plates The BCD vial output number is derived from a formula see below that take plate type plate position and well position into acount BCDValue plate 1 Row 1 Max Col Col 200 BCDValue plate 2 Row 1 Max Col Col 600 1200 Series Autosamplers Service M anual 67 4 Configuring the Autosampler an aps si identificatio iss Qa RFI filter BCD Processor BCD i Line driver 3 250 mA m External External contacts contact connector Figure 23 Block Diagram BCD Board There are general purpose cables available to connect the BCD output see BCD Cables on page 294 and the external outputs see External Contact Cable on page 298 to external devices Table 15 Detailed connector layout 1200 Pin Signal name BCD digit 1 BCD5 20 2 BCD 7 80 3 BCD 6 40 4 BCD 4 10 5 BCD 0 1 6 BCD 3 8 7 BCD 2 4 8 BCD 1 2 9 Digital ground 10 BCD 11 800 11 BCD 10 400 68 1200 Series Autosamplers Service M anual Configuring the Autosampler 4 Table 15 Detailed connector layout 1200 Pin Signal name BCD digit 12 BCD 9 200 13 BCD 8 100 14 not connected 15 5V LAN Communication Interface Board The Agilent 1100 1200 Series modules have one optional board slot that allows to add an interface board to the modules Some modules do not have this interface slot Refer to Agilent 1100 1200 Series Interfaces on page 71 for details
58. Flat screwdriver Partnumber Description G1367 87105 Needle Seat without capillary for G1367D G1367 87303 Optional Seat Capillary 150 mm 0 12 mm ID for G1367 87105 Needle Seat G1367 87302 Optional Seat Capillary 150 mm 0 17 mm ID for G1367 87105 Needle Seat G1377 87101 Needle Seat without capillary for G1377A Optional G1375 87317 Seat Capillary 150 mm 0 10 mm ID for G1377 87101 Needle Seat G1375 87316 Optional Seat Capillary 150 mm 0 075 mm ID for G1377 87101 Needle Seat G1375 87300 Optional Seat Capillary 150 mm 0 05 mm ID for G1377 87101 Needle Seat In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the change needle seat function can be found under the Tools icon Open the front door and remove the side door Remove the plate tray from the tray base Disconnect the seat capillary from the needle seat with the 4 mm open wrench Use the flat head screwdriver to remove the needle seat Insert the new needle seat Press it firmly in position Connect the seat capillary to the needle seat with the 4 mm open wrench Replace the plate tray in the tray base Re install the side door and close the front door In the user interface close the Change Needle Seat function and exit the maintenance mode In the Agilent Lab Monitor amp Diagnostic Software the change needle seat function can be found under the T
59. LC instruments to resolve typical issues Based on chromatographic symptoms Guided Diagnostics helps users to identify the root cause of their problem and leads them through the necessary steps to resolve it LMD also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Monitor amp Diagnostic Software may differ from the descriptions in this manual For details refer to the LMD help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Overview of the Sampler s Indicators and Test Functions Status Indicators The autosampler is provided with two status indicators which indicate the operational state prerun not ready run and error states of the instrument The status indicators provide a quick visual check of the operation of the autosampler see Status Indicators on page 113 Error Messages In the event of an electronic mechanical or hydraulic failure the instrument generates an error message in the user interface For details on error messages and error handling please refer to the Agilent Lab Monitor amp Diagnostic Software Maintenanc
60. M ovement Sensed Reflection Sensor 9 Vial tray identification Reflection Sensor 4 Transport assembly Initialization Table 10 Sampling Unit Flex Board Sensor Type Number of Sensors Position M ovement Sensed IR light sensor 1 Metering device home reference position Reflection sensor 2 Needle end lock positions Microswitch 2 Valve switching Table 11 SLS Board Sensor Type Number of Sensors Position M ovement Sensed Hall sensor 2 Front door closed Table 12 MTP Board Sensor Type Number of Sensors Position M ovement Sensed Hall sensor 2 Left side cover closed 1200 Series Autosamplers Service M anual 57 4 Configuring the Autosampler Microtiter Plate Board MTP 58 Common Electronics A common electronics and firmware design is used for all Agilent 1200 Series LC modules This core design provides a basic set a functions for each module Table 13 Common Electronics Core processor M C68332 Core memory The core unit has 3 memory blocks 2MB SRAM 1MB memory 128 KB NVRAM 24 8 serial NVRAM from the real time clock Communication Interfaces The core unit supports direct the following interfaces CAN bus RS232 Remote MIO LAN ASIC Application Specific Integrated Circuit The application specific integrated circuit ASIC provides interfacing to external devices through drivers including CAN APG Remote It is directly connected to the four control LEDs located near the connectors on this boa
61. Removing the Loop Capillary 191 Installing the Loop Capillary 193 Peristaltic Pump 195 Installing Interface Board 196 167 8 Maintenance Introduction to Maintenance and Repair Simple Repairs Maintenance The autosampler is designed for easy repair The most frequent repairs such as changing a needle assembly can be done from the front of the instrument with the instrument in place in the system stack These repairs are described in Maintenance Procedures on page 175 Exchanging Internal Parts Repairs Some repairs may require exchange of defective internal parts Exchange of these parts requires removing the autosampler from the stack removing the covers and disassembling the autosampler Warnings and Cautions Personal injury Repair work at the autosampler can lead to personal injuries e g shock hazard when the autosampler cover is opened and the instrument is connected to power gt Remove the power cable from the instrument before opening the autosampler cover gt Do not connect the power cable to the autosampler while the covers are removed 168 1200 Series Autosamplers Service M anual Maintenance 8 WARNING Toxic and hazardous solvents The handling of solvents and reagents can hold health risks gt When opening capillary or tube fittings solvents may leak out gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in
62. Repairs 9 Light Protection Kit Installation 1 Remove the transparent front door and install the dark one 2 Slide the transparent window out of the side panel 3 Slide the dark window in the side panel 1200 Series Autosamplers Service M anual 203 9 Repairs Top Cover and Foam CAUTION Electrostatic discharge at the M lO interface board This procedure requires removal of the M lO interface board The board is sensitive to electrostatic discharge gt Always use the ESD strap when handling electronic boards 204 1200 Series Autosamplers Service M anual Repairs 9 Removing the Top Cover and Foam When Tools required Parts required When accessing internal parts Pozidrive screwdriver Flat head screwdriver if interface board installed Partnumber Description 5041 8395 Foam kit includes top and bottom foam Switch off the autosampler at the main power switch Remove the power cable If an interface board is installed remove it see Installing Interface Board on page 196 Move the safety lever to the left Lift the clips on the top cover Lift the cover away Unscrew the three screws on the top plate and remove it Carefully remove the top foam Installing the Top Cover and Foam a ul FW N Install the top foam Press the foam firmly into place Engage the slot on the top plate onto the side plate Fix the top plate in place with the three screws Engage the top cover into the
63. TP board Suggested actions e Check if the side door is installed correctly e Check if the magnet is in place in the side door Do an auto alignment Ensure unobstructed movement of the transport assembly Exchange the sample transport assembly Exchange the sample transport assembly Exchange the defective sampling unit motor Exchange the MTP board 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Metering Home Failed The metering plunger has failed to move back to the home position The home position sensor on the sampling unit flex board monitors the home position of the plunger If the plunger fails to move to the home position or if the sensor fails to recognize the plunger position the error message is generated Probable cause Suggested actions 1 Dirty or defective sensor Exchange the sampling unit flex board 2 Broken plunger Exchange the metering plunger and seal 3 Defective metering drive motor Exchange the metering drive motor 4 Defective MTP board Exchange the MTP board 1200 Series Autosamplers Service M anual 137 7 Troubleshooting and Diagnostics Motor Temperature One of the motors of the transport assembly has drawn excessive current causing the motor to become too hot The processor has switched off the motor to prevent damage to the motor See figure for motor identification e Motor 0 temperature X axis motor e Motor 2 temperature Theta grippe
64. able 3 Physical Specifications sampler G1367B D G1377A Type Specification Comments Weight 15 5 kg 34 2 Ibs Dimensions height x width x depth 200 x 345 x 440 mm 8 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption apparent power 300 VA Maximum Power consumption active power 200 W Maximum Ambient operating temperature 4to 55 C 41 to 131 F Ambient non operating temperature 40 to 70 C 4 to 158 F Humidity lt 95 at 25 to 40 C 77 to 104 F Non condensing Operating Altitude Up to 2000 m 6500 ft Non operating altitude Up to 4600 m 14950 ft For storing the autosampler Safety standards IEC CSA UL Installation Category II Pollution Degree 2 1200 Series Autosamplers Service M anual 29 Site Requirements and Specifications Performance Specifications Table 4 Type GLP features Communications Safety features Injection range Precision Pressure range Sample viscosity range Sample capacity Injection cycle time Carry over Performance specifications Agilent 1200 Series High Performance Autosampler Specification Early maintenance feedback EM F electronic records of maintenance and errors Controller area network CAN RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas
65. ain pass to restore hydraulic flow as it was before the cleaning process The pump s time table can be used if special solvents for cleaning are connected to the pump or if the flushing flow should be adjusted Here s an illustrating example Time B Flow Max Press 1 0 00 100 0 2 0 10 0 500 3 0 11 3 000 4 0 70 100 0 3 000 5 0 71 0 0 0 500 Along with upper injector program this time table uses solvent B for cleaning the seat with an even higher flow rate than the perhaps limiting 0 5ml1 min for column flow To ensure that the flow rate isn t applied to the column and that column doesn t come in contact with flush solvent in this case solvent B choose a considerably long time in this case 90sec for flushing the seat in the injector program Manual procedure If the automatic procedure doesn t succeed there is the semi automatic seat back flushing Preparation e Move the needle to home position e Set pump flow to zero e Connect the seat capillary with the pump capillary using a zero dead volume fitting Flushing e Increase pump flow The seat is flushed backwards solvent bubbles over the seat and leaves the seat via the drainage for the flush port 1200 Series Autosamplers Service M anual Optimizing Performance 6 Optimization for Low est Carry Over Reconfigure system e Set pump flow to zero e Connect the pump outlet capillary to port 1 of injection valve e Connect the seat capillary to port 5 of th
66. ample and mobile phase Organic sample solvents e g DMSO injected into aqueous mobile phase often cause samples to partially deposit on surfaces causing high carry over Chlorhexidine for example dissolved as a free base in methanol and injected into an acidic aqueous mobile phase shows increased carryover than if it is dissolved in 0 1 TFA Since it dissolves slowly but well in acidic aqueous solvents it partially deposits on surfaces during the injection cycle Take care at loop capillary change Push loop capillary forward when tightening the fitting to the needle ensuring gapless transition from loop to needle A replaced needle seat pair may need 100 200 injections for best carry over results Routinely work Prime flush pump for 30 sec with appropriate solvent previous to the first run after usage outage Ensure that the needle seat is not contaminated For cleaning the needle seat see chapter Cleaning the needle seat Use mainpass operation to avoid discrimination of samples 1200 Series Autosamplers Service M anual Optimizing Performance 6 e Notice backpressure of a new column an increase of 10 over time may cause an 10 fold increase in carry over due to the column e Blank vials can be used at least 30 injections Specific recommendations if bypass operation is performed Bypass operation can severely impact carry over performance due to the fact that during gradient operation the sample path is not flus
67. ance feedback EM F electronic records of maintenance and errors Controller area network CAN RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 1 40 ul in 0 1 ul increments Up to 100 ul with extended injection volume hardware modification required Typically lt 0 25 RSD from 5 40 ul Typically lt 0 5 RSD from 2 5 ul Typically lt 0 7 RSD from 1 2 ul volume M easured with injections of benzylalcohol Up to 600 bar 8700 psi 0 2 5cp 2 x well plates MTP 10 x 2 ml vials 108 x 2 mL vials in2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes 0 5 1 5 2 0mL in 2 x 27 Eppendorf tube plate Also compatible with the Agilent 1200 Series sample capacity extension for further expansion of the sample capacity Typically lt 17 s using following standard conditions Default draw speed 100 ul min Default eject speed 100 pl min Injection volume 5 ul Typically lt 0 004 using the following conditions Column Agilent Zorbax SB C18 21x50mm 1 8um P N 827700 902 M obile Phase A H20 0 05 TFA B ACN 0 045 TFA Gradient 0 1min 10 B 3 1 min 90 B 3 2 min 90 B 3 21 10 B 4 5min stop Flow rate 0 5 ml min Temperature 25C Wavelength 257 nm Sample 1200 ng u
68. ary blocked Capillary broken Two 1 4inch 5 16 inch wrenches 8710 0510 supplied in accessory kit Partnumber Description G1367 87300 Loop capillary injection volume up to 100 ul for the G1367B G1377 87310 Loop capillary injection volume up to 40 ul for the G1367D G1375 87315 Loop capillary injection volume up to 8 ul for the G1377A G1377 87300 Loop capillary injection volume up to 40 ul for the G1377A Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be careful when you remove the loop capillary gt Use the silicon safety tube for the needle If the loop capillary is not broken and no solvent has leaked into the loop capillary tube the solvent draw up steps using the syringe can be skipped steps 5 6 8 uF W N O On O In the user interface start the maintenance mode and select the Change Loop Capillary function In the Agilent Lab Monitor amp Diagnostic Software the Change Loop Capillary function can be found under the Tools icon Open the front door and remove the side door Remove the plate tray from the tray base Push the silicon safety tube over the needle Remove the corrugated loop capillary waste tube and introduce the small tubing from the leak kit into the loop capillary protection tube Draw up the liquid with the syringe Unlock the needle tighter lock system Draw up the rest of the solvent from the loop capillary protection tube Loose
69. atus light pipes 278 sheet metal kit 274 vial trays and tray base 257 parts and materials accessory kitSL 259 accessory kit 261 analytical head assembly 269 autosampler sampling unit assembly 267 injection valve assembly 272 injection valve assembly 272 maintenance kit 261 multi draw kit 261 261 sampling unit assembly 266 thermostat 262 transport assembly 254 314 parts and meterials accessory kit micro well plate 260 parts identification cables LAN cables 300 Peekseal 106 performance specifications 30 physical specifications autosampler 29 powercords 27 27 power supply indicator 113 Power Consideration 26 power requirements 26 R radio interference 307 recovering changing serial number and type 239 reflection sensor 57 remote cable 282 287 remote interface 75 repair procedures 171 repairs exchanging internal parts 199 exhaust fan 229 fan 227 injection valve assembly 219 leak sensor 249 main board MTP 232 main cover 202 maintenance procedures 175 metering plunger 188 metering seal 188 metering drive motor and belt 222 MTP board 232 needle assembly 176 needle drive motor and belt 225 powersupply 246 rotorseal 187 sampling unit 211 SLS board 243 SUD board 240 top coverandfoam 204 transportassembly 208 using the ESD strap 169 rotorseal 272 rotorseal 106 272 RS 232C cable kitto PC 299 interface 73 settings 78 S safety information on lithium batteries 305 saftey class 302 sa
70. aw speed 4 ul min Default eject speed 10 pl min Injection volume 0 1 pl Typically lt 0 05 using the following conditions Column 150 x 0 5 mm Hypersil ODS 3 um M obile phase Water Acetonitrile 85 15 Column Flow rate 13 yl min Injection volume 1 ul caffeine 25 ng caffeine 1 ul water to test carryover Outside wash of needle before injection 20 sec with water using flush port 1200 Series Autosamplers Service M anual 1200 Series Autosamplers Service M anual e e 3 Installing the Autosampler Unpacking the Sampler 34 Damaged Packaging 34 Delivery Checklist 34 Accessory Kits 34 Optimizing the Stack Configuration 37 Installing the Autosampler 42 Installing a Thermostatted Autosampler 45 Flow Connections to the Sampler 49 Installing the Sample Tray 51 Transporting the Sampler 53 aa Agilent Technologies 33 3 Installing the Autosampler Unpacking the Sampler If you need to ship the autosampler at a later date always use the shipping protection foam parts see Transporting the Sampler on page 53 Damaged Packaging Upon receipt of your module inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the instrument has been mechanically and electrically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping mate
71. aximum 250 250 1200 Series Autosamplers Service M anual 105 6 Optimizing Performance Choice of Rotor Seal Vespel Seal The standard seal has sealing material made of Vespel Vespel is suitable for applications using mobile phases within the pH range of 2 3 to 9 5 which is suitable for the majority of applications However for applications using mobile phases with pH below 2 3 or above 9 5 the Vespel seal may degrade faster leading to reduced seal lifetime Tefzel Seal For mobile phases with pH below 2 3 or above 9 5 or for conditions where the lifetime of the Vespel seal is drastically reduced a seal made of Tefzel is available Tefzel is more resistant than Vespel to extremes of pH however is a slightly softer material Under normal conditions the expected lifetime of the Tefzel seal is shorter than the Vespel seal however Tefzel may have the longer lifetime under more extreme mobile phase conditions PEEK Seal With the High Performance SL Autosampler a PEEK rotorseal is used This warrants a leak tight system at high pressures and allows the usage of solvents ranging from pH 2 3 to 12 The PEEK material may show a reduced lifetime if used with following solvents e Methylene chloride DMSO e THF e High concentrations of sulfuric acid e High concentrations of nitric acid 106 1200 Series Autosamplers Service M anual Optimizing Performance 6 Choice of Seat Capillary Different models of seat ca
72. bly on page 180 See Exchange the Needle Seat Assembly G1367 87101 on the G1367B Samplers on page 182 See Stator Face on page 186 See Rotor Seal on page 187 See Metering Seal and Plunger on page 188 See Removing the Loop Capillary on page 191 See Peristaltic Pump on page 195 1200 Series Autosamplers Service M anual 175 8 Maintenance Removing the Needle Assembly When Tools required Parts required 1 1 When the needle is visibly damaged When the needle is blocked Two 1 4inch 5 16 inch wrenches 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Partnumber Description G1367 87202 Needle assembly for G1367B D G1377 87201 Needle assembly for G1377A Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be careful when you remove the needle assembly gt Use the silicon safety tube supplied with every new needle 176 gt w on oO Ui In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found in the Tools icon Open the front door and remove the side door Remove the plate tray from the tray base Push the silicon safety tube supplied in the WPS leak kit G1367 60006 and with every new needle over the
73. bly for G1367B 5063 6586 3 Seal support assembly for G1367B 5001 3739 4 Metering seal pack of 2 for G1367B 5063 6589 5 Head body 01078 27710 Screw M5 60 mm lg for mounting of assembly 0515 2118 Table 58 Analytical Head Assembly 40 ul for G1367D Item Description Part Number Micro Analytical head G1377 60023 assembly 40 ul for G1367D includes item 1 6 1 Screws 0515 0850 2 Micro plunger assembly for 5064 8293 G1367D 3 Seal support assembly for G1377 60012 G1367D PEEK backup ring G1377 24001 4 Micro metering seal pack of 1 5022 2175 for G1367D 5 Head body for G1367D G1377 27700 1200 Series Autosamplers Service M anual 269 11 Parts for Repair Table58 Analytical Head Assembly 40 ul for G1367D Item Description Part Number Screw M5 60 mm lg for 0515 2118 mounting of assembly Table 59 Micro analytical head assembly 40 ul for G1377A Item Description Part Number Micro Analytical head assembly 40 ul for G1377A G1377 60013 includes item 1 6 1 Screws 0515 0850 2 Micro plunger assembly for G1377A 5064 8293 3 Micro seal support assembly for G1377A G1377 60002 4 Micro metering seal pack of 1 for G1377A 5022 2175 5 Head body for G1377A G1377 27700 Screw M5 60 mm lg for mounting of assembly 0515 2118 270 1200 Series Autosamplers Service M anual Parts forRepair 11 Analytical Head Assembly 7 COA gt 3 lt gt k 9 Figure 35 Analytical Head Assemb
74. bottom cover Lower the cover into place Ensure the clips close firmly Slide the safety lever to the right and install the power cable 1200 Series Autosamplers Service M anual 205 9 Repairs Illumination Assembly Illumination Assembly When e Defective LEDs Defective internal parts before removing the transport assembly Parts required Partnumber Description 1 G1367 60040 Illumination assembly 1 Remove the top cover top plate and foam see Top Cover and Foam on page 204 2 Disconnect the cable of the illumination assembly at the autosampler mainboard 3 The illumination assembly is located at the top rail of transport assembly After disconnecting the cable from the autosampler mainboard the illumination assembly can be removed by turning the rail approximately 60 degrees 4 Then lift the complete assembly 206 1200 Series Autosamplers Service M anual Repairs 9 Illumination Assembly 5 To install a new illumination assembly position it at the top rail of the transport assembly There are no screws the foam will keep the assembly in place 1200 Series Autosamplers Service M anual 207 9 Repairs b mh t Assembly Transport Assembly 208 1200 Series Autosamplers Service M anual Repairs 9 Removing the Sample Transport When Tools required Parts required Defective transport assembly Defective flex board or sensors 1 4 inch 5 16 inch wrench 8710 0510 supplied in acces
75. capability see Table 3 on page 29 Consequently there is no voltage selector in the rear of the autosampler There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply The thermostatted autosampler comprises two modules the sampler G1367B D or G1377A and the thermostat G1330B Both modules have a separate power supply and a power plug for the line connections The two modules are connected by a control cable and both are turned on by the sampler module The thermostat power supply has two externally accessible fuses Damaged electronics Disconnecting or reconnecting the sampler to thermostat cable w hen the power cords are connected to either of the tw o modules will damage the electronics of the modules gt Make sure the power cords are unplugged before disconnecting or reconnecting the sampler to thermostat cable Incorrect line voltage at the instrument Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your autosampler to the specified line voltage 26 1200 Series Autosamplers Service M anual CAUTION Site Requirements and Specifications 2 Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged
76. cause Suggested actions 1 Fan cable disconnected Ensure the fan is connected correctly 2 Defective fan Exchange fan 3 Defective MTP board Exchange the MTP board 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Front Door Error The front door and or the SLS board are damaged Probable cause Suggested actions 1 The sensor on the SLS board is defective Exchange the SLS board 2 The dooris bent or the magnet is e Exchange the door misplaced broken Exchange the MTP board 1200 Series Autosamplers Service M anual 127 7 Troubleshooting and Diagnostics Side Door Error The side door and or the MTP board are damaged Probable cause Suggested actions 1 The door is bent or the magnet is Change the side door misplaced broken 2 The sensor onthe MTP board is defective Exchange the MTP board 128 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Arm Movement Failed or Arm M ovement Timeout The transport assembly was unable to complete a movement in one of the axes The processor defines a certain time window for the successful completion of a movement in any particular axis The movement and position of the transport assembly is monitored by the encoders on the stepper motors If the processor does not receive the correct position information from the encoders within the time window the error message is generated See figure for axes identification e Arm
77. ccessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Partnumber Description 5062 8590 Needle drive motor 1500 0697 Belt Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 Disconnect the motor connector from the SUD board labelled Metering M Remove the analytical head assembly see Removing the Analytical Head on page 215 Remove the belt the four fixing screws and remove the motor Installing the Needle Lock M otor and Belt 1 Feed the motor cable through to the SUD board Connect the cable to the connector labelled Needle M Secure the motor in place with the four fixing screws install the belt Install the analytical head assembly see Installing the Analytical Head on page 215 Install the top cover top plate and foam see Top Cover and Foam on page 204 Turn ON the sampler close the front door The system will reset automatically 1200 Series Autosamplers Service M anual Repairs 9 Main Fan CAUTION Electrostatic discharge at the MTP board The MTP board is sensitive to electrostatic discharge gt Always use the ESD strap see Using the ESD Strap on page 169 when handling electronic boards 1200 Series Autosamplers Service M anual 227 9 Repairs Removing the Main Fan When Tools req
78. ce limits are still met within the premises 1200 Series Autosamplers Service M anual 307 13 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 308 1200 Series Autosamplers Service M anual Appendix 13 Solvent Information Flow Cell To protect optimal functionality of your flow cell e Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell e Ifthe flow cell is transported while temperatures are below 5 degree C it must be assured that the cell is filled with alcohol e Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add a small of organic solvents e g acetonitrile or methanol 5 Use of Solvents Observe the following recommendations on the use of solvents e Brown glass ware can avoid growth of algae e Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric aci
79. considered not to cap the vial Otherwise small amounts of sample remain on the surface of the septum which may be carried on the needle to the next sample Using the Flush Port During the injection process when the sample is in the loop and when the valve still is in Bypass the outside of the needle can be washed in a flush port located behind the injection port on the sampling unit During the wash cycle as soon the needle is in the flush port a peristaltic pump fills the flush port with fresh solvent during a defined time The volume of the flush port is about 680 ul and the pump delivers 6 ml min Setting the wash time to 10 seconds is sufficient to refill 2 times the flush port In most cases this is enough to clean the needle outside Additionally after the needle left the flush port the flush pump keeps running runs for 6 sec to ensure refill with fresh solvent At the end of this flush process if injection with needle wash is selected the needle returns to the injection port the injection valve is switched to the mainpass position and directs the pump flow back through the sample loop 1200 Series Autosamplers Service M anual Optimizing Performance 6 For further information on how to reduce carry over see Optimization for Lowest Carry Over on page 95 Recommended Wash Solvents e water e ethanol e methanol e water acid especially for basic compounds e water base espeecially for acidic compounds e wa
80. ct The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up A WARNING alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 304 1200 Series Autosamplers Service M anual Appendix 13 Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren Bij dit a
81. d and nitric acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCls O gt gt 2COCly 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA 1200 Series Autosamplers Service M anual 309 13 Appendix 310 Solvent Information Mixtures of carbon tetrachloride with 2 propanol or THF 1200 Series Autosamplers Service M anual Appendix 13 Agilent Technologies on the Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will also provide the latest firmware of the Agilent 1200 series modules for download 1200 Series Autosamplers Service M anual 311 Index Index A accessory kit micro well plate sampler 260 accessory kit high perf autosampler 259 advanced operating modes 20 Agilent Lab M onitor amp Diagnostic
82. d rubber 5181 1210 Silver aluminum Clear PTFE red rubber 5183 4498 1000 Pack Blue aluminum Clear PTFE red rubber 5181 1215 Green aluminum Clear PTFE red rubber 5181 1216 Red aluminum Clear PTFE red rubber 5181 1217 Table 37 Snap Caps Description Septa 100 Pack Clear polypropylene Clear PTFE red rubber 5182 0550 Blue polypropylene Clear PTFE red rubber 5182 3458 Green polypropylene Clear PTFE red rubber 5182 3457 Red polypropylene Clear PTFE red rubber 5182 3459 Table 38 Screw Caps Description Septa 100 Pack Blue polypropylene Clear PTFE red rubber 5182 0717 Green polypropylene Clear PTFE red rubber 5182 0718 Red polypropylene Clear PTFE red rubber 5182 0719 Blue polypropylene Clear PTFE silicone 5182 0720 Green polypropylene Clear PTFE silicone 5182 0721 Red polypropylene Clear PTFE silicone 5182 0722 1200 Series Autosamplers Service M anual Using the Autosampler 5 Configure Well Plate Types If the plate you are using is not found on the List of Recommended Plates and Closing Mat on page 85 you may configure a custom plate Measure the exact dimensions of the plate as marked below and enter the values in the plate configuration table of the ChemStation d column gt Q SF t mF lt I D gt x 2 Mm WG RSE lt gt lt Dea lt r E A B A 7 row x P J gt i me t A D e e Figure26 Well Plate Dimensions straight 1200 Series Autosamplers Service M anual 89 5 Using
83. d the tubing coming from the solvent bottle Connect the wash port tubing to the upper tubing of the new pump use sandpaper to get a good grip on the tubing Connect the tubing coming from the solvent bottle to the lower tubing of the new pump Push the pump onto the motor shaft until the clips click into place Reinstall the corrugated leak tubing 1200 Series Autosamplers Service M anual 195 8 Maintenance Installing Interface Board When Tools required Parts required 1 For all repairs inside the sampler or for installation of the board Flat head screwdriver Description Interface board CAUTION Electrostatic discharge at the interface board The interface board is sensitive to electrostatic discharge gt Always use the ESD strap when handling electronic boards 196 Switch off the autosampler at the main power switch Disconnect all cables from the existing interface board Then loosen the interface board holding screws and slide the board out of its holding rails Identify the interface board slot cover Loosen the two holding screws and remove the cover Carefully insert the new interface board into the holding rails and push the board into the slot Make sure the board plugs into the socket correctly Reconnect all cables to the new interface board Switch on the sampler Configuring optional Interface board see Optional Interface Boards on page 66 1200 Series Autosample
84. d vessel definition in the plate Check the vessel definition in the plate configuration configuration 2 Closing mats to rigid thick Check that the closing mats is not too thick 3 Bad X or Theta positioning Exchange the needle carrier assembly 4 Defective encoder on the needle carrier e Change the sample transport assembly assembly e Change the MTP main board 1200 Series Autosamplers Service M anual 141 7 142 Troubleshooting and Diagnostics Vessel Stuck to Needle The vessel sticks to the needle when the needle moves up Probable cause Suggested actions 1 Closing mat to rigid thick Check that the closing mat is not too thick 2 Bad X or Theta positioning and the needle Exchange the needle carrier assembly sticks into the wall between two holes 3 Defective encoder on the needle carrier e Change the sample transport assembly assembly e Change the MTP main board 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Maintenance Functions Some maintenance procedures require the needle arm metering device and needle carrier to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position In the ChemStation the sampler maintenance positions can be selected from the Maintenance menu in the Diagnosis display In the Control Module the functions can be selected in the Test screens of the auto
85. default settings see Setting the 8 bit Configuration Switch on page 77 7 Turn the module ON again The User Interface should display the correct type for this module 1200 Series Autosamplers Service M anual 239 9 Repairs SUD Board SUD Board CAUTION Damage of the flex board When removing the SUD board the flex board can be damaged gt Remove the SUD board carefully 240 1200 Series Autosamplers Service M anual Repairs 9 Removing the SUD Board When Tools required Parts required When defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Partnumber Description G1313 66503 SUD board In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 Remove the plate tray from the tray base Remove the transport assembly see Removing the Sample Transport on page 209 Remove the sampling unit see Removing the Sampling Unit on page 212 Disconnect all connectors from the SUD board Remove the two fixing screws from the SUD board and lift out the
86. dicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1200 Series Autosamplers Service M anual Contents Contents Introduction 7 Introduction to the 1200 Series Samplers 8 Sampling Sequence 11 Sampling Unit 15 Needle Sample Transport Assembly 18 Advanced Operating Modes 20 Early Maintenance Feedback EMF 22 Electrical Connections 23 Site Requirements and Specifications 25 Site Requirements 26 Physical Specifications 29 Performance Specifications 30 Installing the Autosampler 33 Unpacking the Sampler 34 Optimizing the Stack Configuration 37 Installing the Autosampler 42 Installing a Thermostatted Autosampler 45 Flow Connections to the Sampler 49 Installing the Sample Tray 51 Transporting the Sampler 53 Configuring the Autosampler 55 Autosampler Control and Electronics 56 Position and Movement Sensors 57 Microtiter Plate Board MTP 58 Firmware Description 63 Optional Interface Boards 66 Agilent 1100 1200 Series Interfaces 71 Setting the 8 bit Configuration Switch 77 The Main Power Supply Assembly 82 1200 Series Autosamplers Service M anual Contents 5 Using the Autosampler 83
87. e Vial numheroutp t e io oF ea J m f ol g f E D a fe to ontrol of Thermostat Je A E o LAK O eme E m Tawo cara D o aa Remote aa RS232C DC Out CAN bus Sosa k gt gt gt gt D C c J gt gt gt o C Cd CG CG CG G CO _ gt gt gt gt CG eG p f a_a SS ca K a oo nan _ SS aes Ss SO OS SS e CAN cable to next module Figure 12 Cable Connections NOTE The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The sampler is turned OFF when the line power switch is protruding and the green light is OFF 44 1200 Series Autosamplers Service M anual Installing the Autosampler 3 Installing a Thermostatted Autosampler Parts required Description 1 Sampler and thermostat Power cord Preparations Locate bench space Provide power connections Unpack the sampler and the thermostat WARNING Instrument is partially energized when switched off The power supply still uses some power even if the power switch at the front of the panel is turned off gt To disconnect the sampler from the line unplug the power cord WARNING Damaged electronics Disconnecting or reconnecting the sampler to thermostat cable when the power cords are connected to either of the two modules will damage the electronics of the modules gt Make sure the power cords are un
88. e The peristaltic pump drive is built with a ST L6201 circuit The movement sensing of the valve motor is done by two microswitches Two reflection light sensors are used to detect the end positions of the needle lock device One photo sensor is required to detect the home position of the metering device All the sensors are mounted on one flex board The flex 1200 Series Autosamplers Service M anual Configuring the Autosampler 4 board and motors are connected to the sampling unit distribution board SUD The SUD board is connected to the Microtiter Plate Board MTP via a flat band cable 64 pin Needle Lock Needle Lock Drive End Position 2A Phase Sensors 2 nee Metering Wa Drive Home Position F 247 Phase T ngenar Samping umi FPGA Valve Valve Drive a End Position 4 5A Phase Sensors 2 Peristaltic s ling Uni Pump Sampling Unit Drive 0 15 Distribution Board Sensor Circuits SLS Board Microtiter Safety Hall __ Safety Hall Plate Board Sensors 2 _ Sensors 2 MTP Side Door Front Door Figure 19 Sampling unit control Safety Lock Sensor Board SLS Two hall sensors detect correct closure of the front door needle arm movement is interrupted if front door is open The front door is locked by an electric magnet Unlocking the front door is done by pushing the button on the right side or switching the power off on 1200 Series Autosamplers Service M anual 61 4 Configuring the Autosampler M
89. e Functions The maintenance functions position the needle assembly the needle carrier the sample transport assembly and the metering device for easy access when doing maintenance see Maintenance Functions on page 143 Sample Transport Self Alignment The sample transport self alignment with the sampling unit and the well plate tray is required to compensate for larger deviations in positioning the needle carrier The sample transport self alignment is required after disassembling the system or when you exchange the sample transport the sampling unit the tray or the MTP main board This function is in the diagnose screen of the Chemstation or the Control Module 1200 Series Autosamplers Service M anual 111 7 Troubleshooting and Diagnostics Step Commands The step functions enable execution of each step of the sampling sequence individually The step functions are used primarily for troubleshooting and for verification of correct autosampler operation after repair see High Performance Autosampler Step Commands on page 145 For details on step commands please refer to the Agilent Lab Monitor amp Diagnostic Software 112 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Status Indicators Two status indicators are located on the front of the autosampler The lower left indicates the power supply status the upper right indicates the autosampler status Status indicator green
90. e G1377A sampler has no stator face The injection valve 0101 1422 for the G1367D sampler has no stator face Open the front door Remove all capillaries from the injection valve ports with the 1 4 inch wrench Unscrew and remove the three stator screws from the stator head with the 9 64 inch wrench Remove the stator head the stator face and the stator ring Remove the rotor seal and isolation seal if required Install the new rotor seal and isolation seal if required The metal spring inside the isolation seal must face toward the valve body In other words the metal spring should not be visible when the isolation seal is installed Reinstall the stator ring 1200 Series Autosamplers Service M anual 187 8 Maintenance 8 Place the stator face on the stator head The pins on the stator face must engage in the holes on the stator head 9 Install this stator head face assy on the injection valve Tighten the screws alternately with the 9 64 inch wrench until the stator head is secure 10 Reconnect all the capillaries to the injection valve ports with the 1 4 inch wrench 11 Close the front cover Metering Seal and Plunger 188 1200 Series Autosamplers Service M anual When Tools required Parts required Maintenance 8 Removing the M etering Seal PPP Pe oon ad When poor injection volume reproducibility When leaking metering device 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory k
91. e M anual Configuring the Autosampler 4 Table 28 Stay Resident Settings M ode Select 1 5 7 8 rest BOOT i i o 0 h p o 0 To return to normal operation set switches back to your GPIB or RS 232C configuration settings 1200 Series Autosamplers Service M anual 81 4 Configuring the Autosampler The M ain Power Supply Assembly Table 29 Power Supply Specifications Standard Maximum power Line Input Pin 2 Pin 3 Pin 4 Pin5 Pin 6 Pin7 Pin 8 Pin 9 Pin 10 Pin 11 160 VA 130 W Continuous output 100 240 volts AC 10 line Wide ranging frequency of 50 60 Hz Power Fail error message AGND 15 VDC 15 VDC PGND PGND 24 VDC 24 VDC 36 VDC not used 36 VDC not used DGND 5 VDC 82 1200 Series Autosamplers Service M anual 1200 Series Autosamplers Service M anual e e 5 Using the Autosampler Sample Trays 84 List of Recommended Plates and Closing Mat 85 List of Recommended Vials and Caps 87 Configure Well Plate Types 89 Turn ON and Initialization Steps 92 a Agilent Technologies 83 5 Using the Autosampler Sample Trays Supported Trays for an Autosampler Table 30 Trays for an Autosampler 62258 60011 Tray for 2 well plates or vial plates and 10 x 2 ml vials Pos P2 A1 Vial 10 Pos P2 B1 S Plate 2 Pos P2 P24 Pos P1 A1 Pos P1 B1 S Plate 1 Pos P1 H12 Vial 1 Figure 25 Numbering of vial and well plate position 84 1200 Series Autosamplers Service M a
92. e M anual Repairs 9 Removing the Peristaltic Pump M otor When Tools required Parts required When defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Flat head screwdriver if interface board installed Partnumber Description 5065 4409 Peristaltic pump motor In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 4 Remove the plate tray from the tray base ul wo On cd Remove the transport assembly see Removing the Sample Transport on page 209 Remove the sampling unit see Removing the Sampling Unit on page 212 Remove the air channel Remove the peristaltic pump see Peristaltic Pump on page 195 Remove the base plate of the pump with a small flat head screwdriver 10 Disconnect the peristaltic pump motor from the SUD board labelled mixing M 11 Take out the motor and the holder 12 Unscrew the 2 screws which hold the peristaltic pump motor 13 Remove the peristaltic pump motor Installing the Peristaltic Pump M otor 1 Install the
93. e injection valve e Reset the injector 1200 Series Autosamplers Service M anual 101 6 Optimizing Performance Fast Injection Cycle and Low Delay Volume 102 Fast Injection Cycle and Low Delay Volume General Short injection cycle times for high sample througput is one of the main issues in analytical laboratories Shortening cycle time starts with e shortening column length e high flow rate e steep gradient The detector balance may be set to OFF recommendations for Fast Injection Cycle Times As described in this section the first step to provide short cycle times is optimizing the chromatographic conditions Then the following Autosampler related issues should be considered e Use proper solvent for needle wash to decrease the wash time e Reduce injection volume e Increase eject speed e Increase draw speed if the viscosity of the sample and the solvent in Autosampler s flow path allows it Do injection preparation in parallel with column equilibration section Overlapped Injection after gradient is flushed out Having optimized these parameters further reduction of cycle times can be obtained if column equilibration is short compared to injector preparation or if automated column regeneration is configured Overlapped Injection during run mode decreases this time between runs But note that carry over and discrimination may increase dramatically doing so 1200 Series Autosamplers Service M
94. edit the method or sequence accordingly 3 Incorrect vial positions defined in the Install the correct trays or edit the method or method or sequence sequence accordingly 4 Tray recognition defective dirty sample tray Ensure the coding surfaces of the sample or defective transport assembly flex board tray are clean located at the rear of the sample tray e Exchange the transport assembly 1200 Series Autosamplers Service M anual 139 7 Troubleshooting and Diagnostics Peristaltic Pump Error The peristaltic pump motor in the autosampler has failed The current on the motor is used by the MTP board to monitor the speed of the peristaltic pump motor If the current falls below a certain value the error message is generated Probable cause Suggested actions 1 Defective motor Exchange the peristaltic pump motor 2 Defective SUD board Exchange the SUD board 3 Defective MTP main board Exchange the MTP main board 140 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Vessel or Wash Vessel Error The needle does not reach the target position in the vial or in the vessel of the well plate The sensor behind the vial pusher in the needle carrier assembly detects the successful completion of the needle movement to the vessel If the needle fails to reach the end point the sensor fails to recognize the needle movement and the error message is generated Probable cause Suggested actions 1 Ba
95. en done The autosampler provides the following EMF counters Injection Valve Counter This counter counts the number of valve switching EF4512 since the last reset of the counter Needle Assembly Counter This counter counts the total number of needle into seat movements used for the needle life time EF4510 since the last reset of the counter Seat Assembly Counter This counter counts the total number of needle into seat movements used for the seat life time EF4511 since the last reset of the counter 1200 Series Autosamplers Service M anual 173 8 174 Peristaltic Pump This counter gives the accumulates active pump time in units seconds EF4513 Using the EMF Counters The user setable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The wear of autosampler components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When instrument performance indicates maintenance is necessary make note of the values displayed by the injection valve and needle movements counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zer
96. en the needle carrier moves to a vial and the needle goes into the vial the position of the needle is monitored by an encoder behind the vial pusher If no vial is present the encoder detects an error and the message missing vial is generated Probable cause Suggested actions 1 No vial in the position defined in the method Install the sample vial in the correct position or or sequence edit the method or sequence accordingly 2 Defective needle carrier assembly Exchange the needle carrier assembly 3 Defective transport assembly flex board Exchange the sample transport assembly 4 Defective MTP board Exchange the MTP main board 1200 Series Autosamplers Service M anual 135 7 136 Troubleshooting and Diagnostics _ Initialization Failed The autosampler failed to complete initialization correctly The autosampler initialization procedure moves the needle arm and transport assembly to their home positions in a predefined routine During initialization the processor monitors the position sensors and motor encoders to check for correct movement If one or more of the movements is not successful or is not detected the error message is generated Probable cause 1 y HD UU A Side door not installed correctly Sample transport sampling unit not aligned correctly Mechanical obstruction Defective sampling unit flex board Defective transport assembly flex board Defective sampling unit motor Defective M
97. er Description G1367 87101 Needle Seat assy 0 17 mm ID 2 3 ul for G1367B In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Softwarethe Change Needle Seat function can be found under the Tools icon Open the front door and remove the side door 3 Remove the plate tray from the tray base 182 Disconnect the seat capillary from the injection valve port 5 with the 1 4 inch wrench Use the flat head screwdriver to remove the needle seat Insert the new needle seat assembly G1367 87101 Press it firmly in position Connect the seat capillary to the injection valve port 5 with the 1 4 inch wrench Replace the plate tray in the tray base Re install the side door and close the front door In the user interface close the Change Needle Seat function and exit the maintenance mode In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon 1200 Series Autosamplers Service M anual Maintenance 8 Exchange the Needle Seat G1367 87105 on the G1367D Exchange the Needle Seat G1377 87101 on the G1377A When Tools required Parts required PPP Fee A on O a When the seat is visibly damaged When the seat capillary is blocked 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit
98. eral Purpose Table 80 1100 1200 Green 1 BCD5 20 Violet 2 BCD 7 80 a Blue 3 BCD 6 40 m Yellow 4 BCD 4 10 Black 5 BCD 0 1 Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5 Vt Low 294 1200 Series Autosamplers Service M anual Cable Identification 12 Agilent 1100 1200 to 3396 Integrators Connector03396 60560 Pin 3392 3 Pin Agilent SignalName BCD Digit 1100 1200 1 1 BCD 5 20 2 2 BCD 7 80 A 3 3 BCD 6 40 a 4 4 BCD 4 10 z 3 5 5 BCD 0 1 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 1200 Series Autosamplers Service M anual 295 12 Cable Identification Auxiliary Cable 296 One end of this cable provides a modular plug to be connected to the Agilent 1100 Series vacuum degasser The other end is for general purpose Agilent 1100 Series Degasser to general purposes Table 81 ConnectorG1322 81600 Color Pin Agilent Signal Name 1100 ee White 1 Ground Brown 2 Pressure signal Green 3 Yellow 4 Grey 5 DC 5VIN Pink 6 Vent 1200 Series Autosamplers Service M anual Cable Identification 12 CAN Cable Both ends of this cable provide a modular plug to be connected to Agilent 1100 1200 Series module s CAN bus connectors Table 82 Agilent 1100 1200 module t
99. ers Service M anual Troubleshooting and Diagnostics Errors Which May Occur During the Turn ON and Initialization Process 1200 Series Autosamplers Service M anual 7 149 7 Troubleshooting and Diagnostics Failure to Turn ON No activity when power button is pushed on Power indicator stays off Probable cause Suggested actions 1 Defective main board Turn the sampler off Disconnect the power supply from the main board see if power indicator becomes green when the sampler is turned on e If yes change the main board G1367 69520 2 Power supply defective e Turn the sampler off Disconnect the power supply from the main board see if power indicator becomes green when the sampler is turned on Ifno change the power supply 0905 2528 Rev G or higher 150 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Fan Failure Early during the turn on initialization process a main fan or an exhaust fan error occurs Probable cause Suggested actions 1 Fan not connected to the main board Make sure the fan is connected to the main board correctly 2 Fan connector defective Examine the fan connector for irregularities Correct if possible 3 Fan defective Replace the defective fan main fan 3160 1017 exhaust fan 3160 4097 4 Main board defective Replace the main board G1367 69520 1200 Series Autosamplers Service M anual 151 7 152 Troubleshooting and Diagnostics Main Board
100. es Autosamplers Service M anual Introduction 1 vial plate pusher is an additional passive axis All axes are stepper motor driven and encoder controlled in order to have tight feedback for the axes position The theta and Z axes have spring loaded belt tensioner Reflective light switches detect the presence and type of different trays The X slide carries the antenna and electronics of a RF sensor This device has multiple functions It allows to read and write information from a tag located in the new tray It allows to increase the number of different trays e It allows to read the revision and other data tags of the needle sample transport assembly and sampling unit Complex flex boards make the electrical connection to the various motors sensors and the MTP board The needle carrier has an integrated plate vial pusher with an additional linear encoder to sense vials and the presence of plates The needle and the loop capillary are user exchangeable The back of the needle sample transport assembly has a cover to protect the electronics from potential solvent vapor 1200 Series Autosamplers Service M anual 19 1 Introduction Advanced Operating M odes Multi Draw M ode Optional The multi draw mode provides injection volumes up to 1500 ul In this case a capillary which holds the additional volume is assembled between seat and valve Then the aspirated sample is pushed into the enlarged seat capillary before repet
101. eset automatically 1200 Series Autosamplers Service M anual 231 9 Repairs MTP Main Board CAUTION Electrostatic discharge at the MTP board The MTP board is sensitive to electrostatic discharge gt Always use the ESD strap see Using the ESD Strap on page 169 when handling electronic boards 232 1200 Series Autosamplers Service M anual Repairs 9 Removing the MTP Main Board When Tools required Parts required When defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Partnumber Description G1367 66520 MTP main board G1367 69520 Exchange MTP main board This procedure requires reloading the autosampler firmware and reprogramming the instrument serial number ul oon cd In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 Remove the plate tray from the tray base Remove the transport assembly see Removing the Sample Transport on page 209 Remove the connector screws at the back of the module Remove the M4 screw at the back of the module D
102. from the tray base Remove the transport assembly see Removing the Sample Transport on page 209 On the injection valve remove the capillaries coming from the pump port 1 and going to the column compartment port 6 Use a 1 4 inch wrench if you remove a SST capillary or a 4mm wrench if you remove a fused silica capillary Slide the sampling unit and tray base back to disengage the sampling unit connector Lift the sampling unit and tray base approximately 10 cm Slide the leak sensor out of the leak plane Lift the sampling unit and tray base out of the autosampler 10 Turn the tray base locking screw 1 4 turn anti clockwise 11 Slide the tray base back to disengage the tray base from the sampling unit 1200 Series Autosamplers Service M anual Repairs 9 Installing the Sampling Unit The replacement sampling unit is supplied without injection valve and analytical head assembly If you are exchanging the complete sampling unit remove the injection valve and the analytical head from the defective sampling unit Install the valve and analytical head in the new sampling unit See Injection Valve Assembly on page 219 and M etering Drive M otor and Belt on page 222 9 Mount the tray base onto the sampling unit Ensure the tray base is positioned flush against the sampling unit Tighten the locking screw 1 4 turn clockwise While holding the sampling unit and tray base slide the leak sensor into t
103. ftware the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 Remove the plate tray from the tray base Remove the transport assembly see Removing the Sample Transport on page 209 Remove the sampling unit see Removing the Sampling Unit on page 212 Remove the SLS board cover Disconnect the ribbon cable from the SLS board and unscrew the 2 holding screws Remove the SLS board from the inside of the module Installing the SLS Board uF WwW N Install the new SLS board and the SLS board cover Install the sampling unit see Install the sample transport see Install the top cover top plate and foam see Replace the plate tray in the tray base 1200 Series Autosamplers Service M anual Repairs 9 6 Turn ON the sampler close the front door The system will reset automatically 7 In the user interface perform a Sample Transport Self Alignment In the Agilent Lab Monitor amp Diagnostic Software the Sample Transport Self Alignment can be found under the Calibration icon 1200 Series Autosamplers Service M anual 245 9 Repairs Power Supply CAUTION Electrostatic discharge at the MTP board The MTP board is sensitive to electrostatic discharge gt Always use the ESD strap see Usin
104. g the ESD Strap on page 169 when handling electronic boards 246 1200 Series Autosamplers Service M anual Repairs 9 Removing the Power Supply When Tools required Parts required 8 9 When defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Partnumber Description 0950 2528 rev G and higher only Power supply In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 Remove the plate tray from the tray base Remove the transport assembly see Removing the Sample Transport on page 209 Remove the sampling unit see Removing the Sampling Unit on page 212 Remove the MTP main board see Removing the MTP Main Board on page 233 Remove the fan see Removing the Main Fan on page 228 Remove the bottom foam 10 Remove the exhaust fan see Removing the Exhaust Fan on page 230 11 Remove the power switch light pipe from the coupler 12 Remove the two screws which hold the power supply from the rear panel 13 Lift the power supply
105. ge 204 7 Replace the plate tray in the tray base 8 Turn ON the sampler close the front door The system will reset automatically 9 The system will reset automatically 10 In the user interface perform a Sample Transport Self Alignment In the Agilent Lab Monitor amp Diagnostic Software the Sample Transport Self Alignment can be found under the Calibration icon 1200 Series Autosamplers Service M anual 221 9 Repairs M etering Drive M otor and Belt 222 1200 Series Autosamplers Service M anual Repairs 9 Removing the Metering Drive Motor and Belt When Tools required Parts required When belt or motor defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Partnumber Description 5062 8590 M etering drive motor 1500 0697 Belt In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 4 Remove the plate tray from the tray base Remove the transport assembly see Removing the Sample Transport on page 209 Remove the sampling unit see Removing the Sampl
106. he holder in the leak plane Place the sampling unit and tray base into the autosampler Slide the sampling unit and tray base forwards Ensure the sampling unit connector is seated correctly Install the sample transport assembly see Installing the Sample Transport on page 209 Connect the capillary coming from the pump on port 1 and the one going to the column compartment on port 6 of the injection valve Use a 1 4 inch wrench if you reconnect a SST capillary or a 4 mm wrench if you reconnect a fused silica capillary Install the top cover top plate and foam see Top Cover and Foam on page 204 Replace the plate tray in the tray base 10 Turn ON the sampler close the front door The system will reset automatically 11 The system will reset automatically 12 In the user interface perform a Sample Transport Self Alignment In the Agilent Lab Monitor amp Diagnostic Software the Sample Transport Self Alignment can be found under the Calibration icon 1200 Series Autosamplers Service M anual 213 9 Repairs Analytical Head Analytical Head 214 1200 Series Autosamplers Service M anual Repairs 9 Removing the Analytical Head When Tools required Parts required Pr When defective 4 0 mm 15 cm long T handle hex key 8710 2392 supplied in accessory kit 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory
107. he holder clamp and reinsert the needle assembly into the needle carrier Push the black chain into the needle assembly until the stop Lock the needle tighter lock system Check the alignment of the needle in the needle pusher of the needle carrier by viewing from several directions to see that it is aligned in the center of the needle pusher The needle must be centered in the needle pusher as all alignment by the autosampler is calculated from the needle pusher position 10 Remove the silicon safety tube from the needle 11 Replace the plate tray in the tray base Re install the side door and close the front door 1200 Series Autosamplers Service M anual 193 8 Maintenance 12 In the user interface close the Change Loop Capillary function and exit themaintenance mode In the Agilent Lab Monitor amp Diagnostic Software the Change Loop Capillary function can be found under the Tools icon 194 1200 Series Autosamplers Service M anual Maintenance 8 Peristaltic Pump When Tools required Parts required 1 Tubing blocked or broken sand paper Partnumber Description 5065 4445 Peristaltic pump with Pharmed tubing The peristaltic pump is a replaceable unit The tubing inside the pump is not replaceable AeA W N Pe Remove the corrugated leak tubing Press the two clips on the front of the peristaltic pump Pull the pump forward off the motor shaft Disconnect the tubing leading to the wash port an
108. hed with organic mobile phase This may cause sample discrimination and or adsorption of especially lipophilic components in loop needle and seat flow path The term bypass operation in this context describes all cases where the Autosampler is switched to the Bypass Mode so that the exposure of the Autosampler s internal flow path parts to the solvent flow coming from the pump may become too short This may be the case e when overlapped Injection with option when sample is flushed out is selected e when the delay volume is minimized by using Automated Delay Volume Reduction This mode is not recommended as there may arise two sources for carry over The outlet groove of the injection valve may be contaminated with sample This is the minor issue and can be resolved by performing steps for cleaning the injection valve by method or by injector program The much more problematic issue is that there may remain portions of sample in the sampler Especially if the sample and sample solvent doesn t fit to the mobile phase an arbitrarily large amount of sample may miss the column but stay in the main pass The Injector Purge Kit was developed for this purpose During run the syringe is used as purge pump and afterwards exchanges the purge solvent with gradient start conditions Using this kit decreases the poor bypass carry over significantly Eventually main pass performance may be obtained But the kit does not resolve
109. hen leaking metering device 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit 4mm 15 cm long T handle hex key 8710 2392 supplied in accessory kit Small flat head screwdriver Partnumber Description 5063 6589 Metering Seal pack of 2 for the G1367 60003 100 ul Analytical Head 5063 6586 Metering Plunger for the G1367 60003 100 ul Analytical Head 5022 2175 Metering Seal pack of 1 for the G1377 60013 40 ul Analytical Head 5064 8293 Metering Plunger for the G1377 60013 40 ul Analytical Head Install the new metering seal Press it firmly into position Reassemble the analytical head Press the plunger assembly into the seal Put the two fixing screws in place and reinstall the analytical head to the sampling unit Tighten alternately the two fixing screws with the 4 mm hex key 5 Connect the two capillaries to the analytical head Use a 1 4 inch wrench if you have a SST capillary or a 4 mm wrench if you have a fused silica capillary Reinstall the corrugated leak tubing Close the front door In the user interface close the Change Piston function and exit the maintenance mode In the Agilent Lab Monitor amp Diagnostic Software the Change Piston function can be found under the Tools icon 1200 Series Autosamplers Service M anual Maintenance 8 Removing the Loop Capillary When Tools required Parts required 1 1 1 1 Capill
110. icrotiter Plate Board MTP 1 0 signals TPU signals 8 bit data 5 bit address FPGA chip select Encoder clocks and direction Quadrature decoder Limit switch signals a y p Injection Valve p o a p Metering Device __p FPGA p Needle Lock Device p ea kp Peristaltic Pump 8 bit data 4 X Axis 4 P Z Axis le 4 Encoder feedback data theta Figure 20 Autosampler block diagram 62 1200 Series Autosamplers Service M anual Configuring the Autosampler 4 Firmware Description The firmware of the instrument consists of two independent sections e anon instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1200 series modules Its properties are e the complete communication capabilities CAN LAN and RS 232C e memory management e ability to update the firmware of the main system Main System Its properties are e the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote e error handling e diagnostic functions e or module specific functions li
111. ific initialization procedures Factory setting is shown for the variable wavelength detector Table 21 8 bit Configuration Switch M ode Select GPIB RS 232C Reserved JTEST BOOT 1 1 Baud rate Reserved RSVD 5 GPIB address bys Data its Rsvo Parity RSVD FC 4 Switches 1 and 2 define which set of parameters for example for GPIB RS 232C and so on will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory 1200 Series Autosamplers Service M anual 77 4 78 Configuring the Autosampler In the non volatile memory the parameters are kept regardless of whether you turn the instrument OFF and ON again They will be kept until the same set of parameters is changed and the power is reset All other previously stored configuration settings will still remain in the non volatile memory In this way you can store more than one set of parameters using the same 8 bit configuration switch twice for example for both GPIB and RS 232C GPIB Default Addresses If you just want to change the GPIB address and need a detailed procedure refer to the Installing Your Agilent ChemStation System handbook Default GPIB address is set to the following addresses Table 22 Default Addresses for Agilent Series 1200 Modules Module Address Binary Address Pump 22 00010110 FLD 23 00010111 VW
112. iled open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the MTP Ensure the leak sensor is connected correctly board 2 Defective leak sensor Exchange the leak sensor 3 1200 Series Autosamplers Service M anual 121 7 122 Troubleshooting and Diagnostics Leak Sensor Short The leak sensor in the autosampler has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Exchange the leak sensor 2 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Compensation Sensor Open The ambient compensation sensor NTC on the MTP board in the autosampler has failed open circuit The resistance across the temperature compensation sensor NTC on the MTP board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor i
113. ing Unit on page 212 Disconnect the motor connector from the SUD board labelled Metering M Remove the four screws which fix the motor Remove the motor Remove the belt and place it over the shaft Installing the M etering Drive M otor and Belt 1 Feed the motor cable through to the SUD board Connect the cable to labelled Metering M Install the motor with the four fixing screws 3 Ensure the belt is seated correctly over the gear and motor shaft Install the sampling unit see Installing the Sampling Unit on page 213 1200 Series Autosamplers Service M anual 223 9 Repairs 5 Install the sample transport see Installing the Sample Transport on page 209 6 Install the top cover top plate and foam see Top Cover and Foam on page 204 7 Replace the plate tray in the tray base 8 Turn ON the sampler close the front door The system will reset automatically 9 In the user interface perform a Sample Transport Self Alignment In the Agilent Lab Monitor amp Diagnostic Software the Sample Transport Self Alignment can be found under the Calibration icon 224 1200 Series Autosamplers Service M anual Repairs 9 Needle lock M otor and Belt 1200 Series Autosamplers Service M anual 225 9 When Repairs Removing the Needle Lock M otor and Belt Tools required Parts required 226 When defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in a
114. ing drive motor Needle Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Draw Sum of all draw volumes exceeds 100 ul or 40 ul Defective metering drive motor Needle Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defect ive needle drive motor 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Table 43 Step Failures Step Function Probable Failure M odes Valve M ainpass Valve not connected Defective injection valve Needle Up M ainpass Blockage in the sample loop or needle no solvent flow Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Valve not connected Defective injection valve 1200 Series Autosamplers Service M anual 147 7 Troubleshooting and Diagnostics Troubleshooting the G1367B D and G1377A Autosampler Gather Information About the Problem e When did the problem start e What was done changed prior to the start of the problem In the Agilent Lab Monitor amp Diagnostic Software the Instrument Status Report generates a report This report includes the Instrument configuration with the instrument serial numbers and the firmware revisions the instrument error history the EMF editor the result of the guided diagnostic and the method parameter optional 148 1200 Series Autosampl
115. isconnect all connectors on the MTP board Slide the MTP main board out of the sampler Installing the MTP Main Board 1 Install the new board Ensure the ribbon cables are positioned in the slot in the board 1200 Series Autosamplers Service M anual 233 9 Repairs Reconnect the connectors to the board Replace the connector screws in the back of the module Replace the M4 screw at the back of the module uF W N Install the sample transport see Installing the Sample Transport on page 209 6 Install the top cover top plate and foam see Top Cover and Foam on page 204 7 Replace the plate tray in the tray base 8 Turn ON the sampler close the front door the system will reset automatically If a new sampler main board is installed update the serial number information of the sampler in the user interface see procedure below 9 Check the firmware revision of the sampler If the firmware revision is older than the current firmware revision of the autosampler update the firmware 10 Perform a Sample Transport Self Alignment In the Agilent Lab Monitor amp Diagnostic Software the Sample Transport Self Alignment can be found under the Calibration icon 234 1200 Series Autosamplers Service M anual Repairs 9 Introduction Introduction When the main board has to be replaced the new board does not have a serial number For some modules e g pumps or auto samplers the type has
116. it 4mm open end wrench 8710 1534 supplied in accessory kit 4mm 15 cm long T handle hex key 8710 2392 supplied in accessory kit Small flat head screwdriver Partnumber Description 5063 6589 Metering Seal pack of 2 for the G1367 60003 100 ul Analytical Head 5063 6586 Metering Plunger for the G1367 60003 100 ul Analytical Head 5022 2175 Metering Seal pack of 1 for the G1377 60013 40 ul Analytical Head 5064 8293 Metering Plunger for the G1377 60013 40 ul Analytical Head In the user interface start the maintenance mode and select the Change Piston function In the Agilent Lab Monitor amp Diagnostic Software the Change Piston function can be found under the Tools icon Open the front door Remove the corrugated leak tubing Remove the two capillaries from the analytical head Use a 1 4 inch wrench if you have a SST capillary or a 4 mm wrench if you have a fused silica capillary Unscrew alternately the two fixing screws with the 4 mm hex key and remove them Pull the analytical head away from the sampling unit Remove the two fixing screws from the base of the analytical head Remove the head body Using the piston carefully remove the metering seal Clean the chamber and ensure all particular matter is removed 1200 Series Autosamplers Service M anual 189 8 Maintenance When Tools required Parts required 190 Installing the M etering Seal PPP Pe When poor injection volume reproducibility W
117. itiate appropriate actions 1 Install the LAN interface board in the sampler if required 2 Remove the adhesive tape which covers the side and front doors 42 1200 Series Autosamplers Service M anual xn oO oO FP UW 8 9 Installing the Autosampler 3 Open the front door and remove the left side door Remove the transport protection foam Re install the corrugated waste tube in the plastic port Re install the left side door take care of the magnet at the back Place the autosampler in the stack or on the bench in all horizontal position Ensure the power switch at the front of the sampler is OFF Connect the power cable to the power connector at the rear of the sampler 10 Connect the CAN cable to the other Agilent 1200 modules 11 If a Agilent ChemStation is the controller connect the LAN connection to the LAN interface 12 Connect the APG remote cable optional for non Agilent 1200 Series instruments 13 Ensure the side panel is correctly installed 14 Turn ON power by pushing the button at the lower left hand side of the sampler 1200 Series Autosamplers Service M anual 43 3 Installing the Autosampler Installing the Autosampler 15 Close the front door The exhaust fan will turn ON and remove the vapor from the tray compartment After 1 2 minutes the sampler will start the hardware initialisation process At the end of this process the status LED should be off CAN cable to previous modul
118. itive aspiration starts After the last aspiration took place the injection valve switches and the mobile phase transports sample towards column Injector Program A sequence of all available single sampling steps can be tailored to customer needs for special applications Injector program capability is offered with the standard instrument Active Needle Wash The active needle wash mode allows also the flushing of the outer surface of the needle This results in an additional decrease of sample carry over Duration of the procedure is setable Overlap Injection Cycle Overlapped injection is the mode where the autosampler runs the injector program for the next analysis during the current analysis without injecting After the sample has reached the column the valve is switched back to bypass and the next injection cycle starts but waits with switching to main pass until the actual run is finished This mode allows it to increase the sample throughput 20 1200 Series Autosamplers Service M anual Introduction 1 Low Delay Volume M ode This mode is especially interesting for gradient elution with small bore or capillary columns The injection valve is switched back to bypass after the sample is eluted beyond the injection valve port 6 This decreases the delay volume because the gradient needs not to pass the metering device and the loop capillary 1200 Series Autosamplers Service M anual 21 1 Introduction Early Maintena
119. jection 0101 1409 valve purge kit setup Restriction capillary for injection valve waste outlet G1373 87300 CAN cable 1 m long for 1200 Series module to module CAN 5181 1519 connections DC CAN cable to supply power to the G1156A valve frome g 5181 1533 the G1367B WP ALS Rheotool socket wrench 8710 2391 1200 Series Autosamplers Service M anual Parts and Materials for Maintenance 10 Table 54 Injector Purge Kit Parts for G1367B Autosamplers Description Part Number 9 64 Hex key driver 8710 2394 Priming Syringe for solvent inlet tubing reorder number 5062 8534 pack of 10 Syringe Adapter for priming syringe 9301 1337 ChemStation CD ROM Rev B 01 03 or higher for the use of N A this kit Installation Note this note N A 1200 Series Autosamplers Service M anual 263 10 Parts and Materials for Maintenance Thermostat for ALS FC Spotter Thermostat for ALS FC Spotter Table 55 Thermostat for ALC FC Spotter Item Description Part Number 1 Thermostat exchange assembly G1330 69040 Figure 33 Thermostat for ALS FC Spotter 264 1200 Series Autosamplers Service M anual 1200 Series Autosamplers Service M anual 11 Parts for Repair Sampling Unit Assembly 266 Analytical Head Assembly 269 Injection Valve Assembly 272 Sheet Metal Kit 274 Cover Parts 275 Foam Parts 276 Power and Status Light Pipes 278 Leak System Parts 279 ee Agilent Technologies 265 11 Parts for Re
120. jection Cycle and Low Delay Volume 102 Precise Injection Volume 104 Draw and Eject Speed 104 Choice of RotorSeal 106 Choice of Seat Capillary 107 att Agilent Technologies 93 6 Optimizing Performance Optimizing Performance 94 Autosamplers are more and more used in HPLC to improve the productivity in the laboratories and the consistency and accuracy of analytical results The informations below will help you on how to optimize some parameters to achieve best results for lowest carry over for reliable quantitative data e Fast injection cycles for high throughput Low delay volume for fast gradient e Precise injection volume Specific information for optimizing the performance of the Agilent 1200 series high performance autosampler SL can be found in the Agilent 1200 Series Rapid Resolution LC System User Manual 1200 Series Autosamplers Service M anual Optimizing Performance 6 Optimization for Lowest Carry Over Optimization for Lowest Carry Over Carry over CO on 1100 1200 Agilent system is not only a topic for injection systems but may have multiple sources Hardware related sample loop needle outside needle inside needle seat seat capillary injection valve flush time wash vials fittings column carry over depends on frit design material blockage surface activity of frits capillaries Chemistry Physics related suitable sample solvent has to be compatible with mobile phase
121. ke internal events such as lamp control filter movements raw data collection and conversion to absorbance Firmware Updates Firmware updates can be done using your user interface e PC and Firmware Update Tool with local files on the hard disk or 1200 Series Autosamplers Service M anual 63 4 64 Configuring the Autosampler e Instant Pilot G4208A with files from a USB Flash Disik or e handheld control module G1323A B with files from a PC card The file naming conventions are PPPP_RVVV_XX dlb where PPPP is the product number for example 1315AB for the G1315A B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 102 is revision 1 02 XX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main FW update Resident System Main System Resident FW Update Figure 21 Firmware Update M echanism 1200 Series Autosamplers Service M anual Configuring the Autosampler Some 1200 series modules are limited in downgrading due to their main board version or their inititial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 0
122. kit Partnumber Description G1367 60003 Analytical Head assembly 100 ul for G1367B G1377 60023 Analytical Head assembly 40 ul for G1367D G1367 60013 Analytical Head assembly 40 ul for G1377A In the user interface start the maintenance mode and select the Change Piston function In the Agilent Lab Monitor amp Diagnostic Software the Change Piston function can be found under the Tools icon Remove the two capillaries from the analytical head Use a 1 4 inch wrench if you have a SST capillary or a 4 mm wrench if you have a fused silica capillary Alternately unscrew the two hex screws which maintain the analytical head with the 4 mm hex key Remove the analytical head Installing the Analytical Head Install the new analytical head on the sampling unit Install and tighten alternately the two hex keys which fix the injection valve assembly 3 Alternately tighten the two fixing screws with the 4 mm hex key Connect the two capillaries on the analytical head Use a 1 4 inch wrench if you have a SST capillary or a 4 mm wrench if you have a fused silica capillary Close the front door In the user interface close the Change Piston function and exit the maintenance mode In the Agilent Lab Monitor amp Diagnostic Software the Change Piston function can be found under the Tools icon 1200 Series Autosamplers Service M anual 215 9 Repairs Peristaltic Pump M otor 216 1200 Series Autosamplers Servic
123. l Chlorhexidine disolved in H20 with 0 1 TFA lul injected and measure both on Agilent 6410 QQQ and G1315C DAD Wash solution H20 with 0 1 TFA 5 sec 2 1200 Series Autosamplers Service M anual 31 Site Requirements and Specifications Table 6 Performance Specifications Agilent 1200 Series Micro Well Plate Autosampler Type Specification GLP features Communications Safety features Injection range Precision Pressure range Sample viscosity range Sample capacity Injection cycle time Carry over Early maintenance feedback EM F electronic records of maintenance and errors Controller area network CAN RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 01 8 ul in 0 01 ul increments with the small loop capillary 0 01 40 wl in 0 01 ul increments with the extended loop capillary Typically lt 0 5 RSD of peak areas from 5 40 ul Typically lt 1 RSD from 1 5 ul Typically lt 3 RSD from 0 2 1 ul up to 400 bar 5880 psi 0 2 5cp 2 x well plates MTP 10 x 2 ml vials 108 x 2 mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes 0 5 1 5 2 0 mL in 2 x 27 Eppendorf tube plate Typically lt 30 s using following standard conditions Default dr
124. l adapter 1 G1329 43200 Capillary WPS to column 250 mm 0 17 mm ID 1 G1367 87304 WPS leak kit 1 G1367 60006 Tool for micro seat capillary 1 61377 44900 Table 8 Micro well plate Autosampler Accessory Kit G1377 68705 Description Quantity Part Number 96 well plate 0 5 ml PP pack of 10 1 5042 1386 Tubing assembly 1 5063 6527 Filter kit 1 5064 8240 CAN cable 1m 1 5181 1519 Vials screw cap 100 pk 1 5182 0716 1200 Series Autosamplers Service M anual 35 3 36 Installing the Autosampler Table 8 Micro well plate Autosampler Accessory Kit G1377 68705 Description Quantity Part Number Blue screw caps 100 pk Hex key 9 64 inch for injection valve screws Wrenches 1 4 5 16inch Wrench 4 0 mm open end Rheotool socket wrench 1 4 inch Hex key 4 0 mm 15 cm long T handle Hex key 9 64 inch 15 cm long T handle Hex key 2 5 mm 15 cm long straight handle Hex key 2 0 mm Torque adapter Air channel adapter Capillary sampler column 500 mm 0 05 mm ID 40 ul Loop capillary WPS leak kit Seat capillary 150 mm 0 075 mm ID Tool for micro seat capillary 1 5182 0717 1 8710 0060 2 8710 0510 1 8710 1534 1 8710 2391 1 8710 2392 1 8710 2394 1 8710 2412 1 8710 2438 1 G1315 45003 1 G1329 43200 1 G1375 87304 1 G1377 87300 1 G1367 60006 1 G1367 87316 1 G1377 44900 1200 Series Autosamplers Service M anual Installing the Autosampler 3 Optimizing the Stack Configuration If your aut
125. lable as a spare part and is recommended for applications with a 0 3 mm column or smaller This capillary gives full chromatographic performance Due to the small diameter this capillary can show some blockage 108 1200 Series Autosamplers Service M anual 1200 Series Autosamplers Service M anual 7 Troubleshooting and Diagnostics Agilent Lab Monitor amp Diagnostic Software 110 Overview of the Sampler s Indicators and Test Functions 111 Status Indicators 113 Power Supply Indicator 113 Instrument Status Indicator 113 ErrorMessages 115 Maintenance Functions 143 Sample Transport Self Alignment 144 High Performance Autosampler Step Commands 145 Troubleshooting 146 Troubleshooting the G1367B D and G1377A Autosampler 148 Errors Which M ay Occur During the Turn ON and Initialization Process 149 Instrument Logbook Errors and Step by Step RepairProcess 156 Needle Centering Over the Vial orthe Well 166 ee Agilent Technologies 109 7 Troubleshooting and Diagnostics Agilent Lab M onitor amp Diagnostic Software 110 The Agilent Lab Monitor amp Diagnostic Software LMD is an application independent tool that provides diagnostic capabilities for all Agilent 1200 Series HPLC modules It includes tests calibrations and tools such as injector steps and maintenance positions In addition to these features of the LMD Basic edition the Advanced edition of LMD provides Guided Diagnostics which interactively supports users of
126. le Carrier Assembly When When the needle carrier is defect Tools required e 2mm hex key 8710 2438 supplied in accessory kit Parts required Partnumber Description 1 G1367 60010 Needle Carrier assembly WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be carefull when you remove the needle carrier assembly gt Use the silicon safety tube supplied with every new needle 1 In the user interface start the maintenance mode and select the Change Needle Carrier function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Carrier function can be found in the Tools icon 2 Open the front door and remove the side door 3 Remove the plate tray from the tray base 4 Push the silicon safety tube supplied in the WPS leak kit G1367 60006 and with every new needle over the needle 5 Pinch the holder clamp pull back and remove the needle assembly from the needle carrier 6 Unplug the flex board on the sample transport 7 Unscrew the three holding hex screws with the 2 mm hex key 8 Remove the needle carrier assy 180 1200 Series Autosamplers Service M anual Maintenance 8 Installing the Needle Carrier Assembly When When the needle carrier is defect Tools required Parts required 1 PWN rPe 2 mm hex key 8710 2438 supplied in accessory kit Partnumber Description G1367 60010 Needle Carrier assembly Install a new needle carrie
127. le times also depend on the injection volume In identically standard condition injecting 100 ul instead of 1 ul increase the injection time by approximately 8 sec In this case and if the viscosity of the sample allows it the draw and eject speed of the injection system has to be increased 1200 Series Autosamplers Service M anual 103 6 Optimizing Performance Precise Injection Volume 104 _ Injection Volumes Less Than 2 pl When the injection valve switches to the BYPASS position the mobile phase in the sample loop is depressurized When the syringe begins drawing sample the mobile phase is further subjected to decreasing pressure If the mobile phase is not adequately degassed small gas bubbles may form in the sample loop during the injection sequence When using injection volumes lt 2 ul these gas bubbles may affect the injection volume precision For best injection volume precision with injection volumes lt 2 ul use of the Agilent 1200 Series degasser is recommended to ensure the mobile phase is adequately degassed Also using the automated needle wash see Using the Automated Needle Wash on page 98 between injections reduces carry over to a minimum improving injection volume precision further Draw and Eject Speed Draw Speed The speed at which the metering unit draws sample out of the vial may have an influence on the injection volume precision when using viscous samples If the draw speed is too high
128. led to wash the outside of the needle This reduces the already low carry over for very sensitive analysis The bottle containing the mobile phase for the wash procedure will be located in the solvent bottle cabinet Produced waste during this operation is channeled safely away through a waste drain 1200 Series Autosamplers Service M anual 9 1 10 Introduction The six port only 5 ports are used injection valve unit is driven by a high speed hybrid stepper motor During the sampling sequence the valve unit bypasses the autosampler and connects flow from the pump to the column directly During injection and analysis the valve unit directs the flow through the autosampler which ensures that all of the sample is injected onto the column and that the metering unit and needle are always free from sample residue before the next sampling sequence begins All the injection valves have different stator heads and different rotor seals The volume of each valve is different Control of the vial plate temperature in the thermostatted autosampler is achieved using an additional Agilent 1200 Series module the Agilent 1200 Series thermostat for ALS FC Spotter The thermostat contains Peltier controlled heat exchangers A fan draws air from the area above the sample vial tray of the autosampler It is then blown through the fins of the cooling heating module There it is cooled or heated according the temperature setting The thermostatted air ente
129. llary from the seat with the 4 mm wrench Install the seat assembly in its location and reconnect the capillary to the injection valve port 5 Follow the procedure in Exchange the Needle Seat G1367 87105 on the G1367D Exchange the Needle Seat G1377 87101 on the G1377A on page 183 to finish the installation Choose the seat capillary diameter as function of the column and the application you run on the system See Choice of Seat Capillary on page 107 1200 Series Autosamplers Service M anual 185 8 Maintenance Stator Face When Tools required Parts required CAUTION 1 When poor injection volume reproducibility When leaking injection valve 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 9 64 inch 15 cm long T handle hex key 8710 2394 supplied in accessory kit Partnumber Description 0100 1851 Stator Face Damage of the stator face The stator face is held in place by the stator head When removing the stator head the stator face can be damaged gt When removing the stator head ensure the stator face does not fall out of the valve 186 This procedure is only for the injection valve on the G1367B samplers The injection valve on the G1367D and the G1377A samplers have no ceramic stator face Open the front door Remove all capillaries from the injection valve ports with the 1 4 inch wrench Unscrew and remove the three stator screws from the stator head with
130. ly 100 ul or 40 ul 1200 Series Autosamplers Service M anual 271 11 Parts for Repair Injection Valve Assembly Table 60 Injection valve assembly for G1367B Item Description Part Number 1 Injection valve assembly includes items 1 6 0101 0921 2 Isolation seal 0100 1852 3 Rotor seal Vespel 0100 1853 3 Rotor seal Tefzel 0100 1849 3 Rotor seal PEEK 0100 2231 4 Stator face 0100 1851 5 Stator head 0100 1850 6 Stator screws 1535 4857 Table61 Micro injection valve for G1377A The M icro injection valve assembly has no ceramic stator face Item Description Part Number 1 Micro injection valve assembly incl items 1 2 3 5 6 0100 1050 2 Isolation seal 0100 1852 3 M icro Rotor seal Vespel 0100 2088 includes 3 screws 5 M icro stator head 0100 2089 6 Stator screws 1535 4857 272 1200 Series Autosamplers Service M anual Parts forRepair 11 Table 62 _Injection Valve Assembly for G1367D 600 bar The injection valve assembly for G1367D has no ceramic stator Item Description Part Number 1 Injection valve assembly includes items 1 6 0101 1422 2 Isolation seal 0100 1852 3 Rotor seal PEEK 0101 1416 includes 3 screws 5 Stator head 0101 1417 6 Stator screws 1535 4857 Figure 36 Injection Valve Assembly 1200 Series Autosamplers Service M anual 273 11 Parts for Repair Sheet M etal Kit Sheet M etal Kit Table 63 Sheet Metal Item Description PartNr 1 Slot cover 5001 377
131. ly for the G1367B 0101 1422 Injection Valve assembly for the G1367D 0101 1050 Micro Injection valve assembly for the G1377A In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 Remove the plate tray from the tray base Remove the transport assembly see Removing the Sample Transport on page 209 Remove the sampling unit see Removing the Sampling Unit on page 212 Disconnect the valve cable connector from the SUD board Valve Assy Remove the three screws which fix the injection valve assembly Remove the injection valve assembly Installing the Injection Valve Assembly PrP WwW N Feed the cable of the new valve through to the SUD board Connect the valve cable to the connector labelled valve assy Install and tighten the three screws which fix the injection valve assembly Install the sampling unit see Installing the Sampling Unit on page 213 1200 Series Autosamplers Service M anual Repairs 9 5 Install the sample transport see Installing the Sample Transport on page 209 6 Install the top cover top plate and foam see Top Cover and Foam on pa
132. mechanism is secured with the transport protection foam 1200 Series Autosamplers Service M anual 53 3 Installing the Autosampler Transporting the Sampler 54 1200 Series Autosamplers Service M anual 1200 Series Autosamplers Service M anual e e 4 Configuring the Autosampler Autosampler Control and Electronics 56 Position and Movement Sensors 57 Microtiter Plate Board MTP 58 Autosampler Specific Electronics 59 Firmware Description 63 Optional Interface Boards 66 BCD Board 66 LAN Communication Interface Board 69 Agilent 1100 1200 Series Interfaces 71 Setting the 8 bit Configuration Switch 77 The Main Power Supply Assembly 82 a Agilent Technologies 55 4 Configuring the Autosampler Autosampler Control and Electronics The Microtiter Plate Board MTP controls the vial transport mechanism sampling needle metering unit and high speed injection valve These devices are controlled by a versatile electronics design based upon a 68000 family processor which also contains battery backup RAM flash ROM a real time clock and several communications options 56 1200 Series Autosamplers Service M anual Position and M ovement Sensors Configuring the Autosampler 4 Position sensing of movement of autosampler components is done by sensors on the sample transport and sampling unit flex boards The following sensors are used Table 9 Sample Transport Flex Board Sensor Type Number of Sensors Position
133. mple trays 84 numbering of vial positions 85 sampling sequence 11 sampling unit 15 seals 106 metering seal 269 rotor seal 272 rotor seal 272 seat capillary 107 sensors 57 serial number type using ChemStation 236 using Control M module 238 using Instant Pilot 237 using LM D software 235 serial number changing 235 235 shipping containers 34 Shipping 53 shut down 117 site requirements 26 solvent information 309 1200 Series Autosamplers Service M anual Index solvents 309 sound emission 308 special interfaces 76 specifications 29 30 stack configuration rearview 39 rearview 41 thermostatted autosampler 41 stator 16 status indicator 111 Status indicators 113 Stay Resident settings 80 step functions 112 step commands 145 Storage 28 Switch configuration settings 77 sychronization lost 118 T Tefzel seal 106 temperature sensor 120 temperature 28 30 thermostat 264 theta axis 18 timeout 116 transport assembly 18 transport mechanism 8 transport 53 transporting the autosampler 53 tray combinations 85 trays 84 type changing 235 235 U unpacking the autosampler 34 V valve capillaries 49 Vespel seal 106 vial contents temperature 30 vial numbering 84 vialtray 53 vialracks 8 vials 8 viscous samples 104 104 W weight 28 X X axis 18 Z Z axis 18 1200 Series Autosamplers Service M anual 315 www agilent com In This Book This manual contains service information about the Agile
134. n the loop capillary fitting on the analytical head side 1200 Series Autosamplers Service M anual 191 8 Maintenance 10 Pinch the holder clamp pull back and remove the needle assembly with the loop capillary from the needle carrier 11 Attach the 5 16 inch wrench to hold position at the needle assembly Use the 4 mm wrench to loosen the fitting of the loop capillary 12 Pull the loop capillary out from the needle assembly 192 1200 Series Autosamplers Service M anual Maintenance 8 Installing the Loop Capillary When Tools required Parts required Capillary blocked Capillary broken PrP PE m Two 1 4inch 5 16 inch wrench 8710 0510 supplied in accessory kit Partnumber Description G1367 87300 Loop capillary injection volume up to 100 ul for the G1367B G1377 87310 Loop capillary injection volume up to 40 ul for the G1367D G1375 87315 Loop capillary injection volume up to 8 ul for the G1377A G1377 87300 Loop capillary injection volume up to 40 ul for the G1377A Pull the new loop capillary in the needle assembly Attach the 5 16 inch wrench to hold position at the needle assembly Use the second wrench to tighten the loop capillary fitting Push the loop capillary into the loop capillary protection tube until it comes out on the sampling unit side 4 Re install the loop capillary corrugated waste tube over the loop capillary Retighten the loop capillary fitting on the analytical head Pinch t
135. nce Feedback EMF 22 The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled For details on EMF counters and how to use them see LMD Lab Monitor and Diagnostic software documentation 1200 Series Autosamplers Service M anual Introduction 1 Electrical Connections Electrical Connections CAN cable to previous module Vial number output 7 Relay contacts 7 Ses F qo Ct ontrol of Thermostat s Sa ea aA y o ume Tawo 0 wg p comma mD D m Remote RS232C DC Out CAN bus CAN cable to next module Figure7 Autosampler Electrical Connections 1200 Series Autosamplers Service M anual 23 1 24 Introduction Electrical Connections 1200 Series Autosamplers Service M anual 1200 Series Autosamplers Service M anual e 2 Site Requirements and Specifications Site Requirements 26 Power Consideration 26 PowerCords 27 Bench Space 28 Environment 28 Physical Specifications 29 Performance Specifications 30 ee Agilent Technologies 25 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimum performance of the autosampler Power Consideration The autosampler power supply has wide ranging
136. ncreases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective MTP board Exchange the MTP board 1200 Series Autosamplers Service M anual 123 7 124 Troubleshooting and Diagnostics Compensation Sensor Short The ambient compensation sensor NTC on the MTP board in the autosampler has failed short circuit The resistance across the temperature compensation sensor NTC on the MTP board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective MTP board Exchange the MTP board 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Fan Failed The cooling fan in the autosampler has failed Probable cause Suggested actions 1 Fan cable disconnected Ensure the fan is connected correctly 2 Defective fan Exchange fan 3 Defective MTP board Exchange the MTP board 4 1200 Series Autosamplers Service M anual 125 7 126 Troubleshooting and Diagnostics Exhaust Fan Failed The exhaust fan in the autosampler has failed The hall sensor on the fan shaft is used by the MTP board to monitor the fan speed If the fan speed falls below a certain value the error message is generated and the autosampler shuts down Probable
137. nd moved onto the seat to close the sample loop Sample is applied to the column when the injection valve returns to the mainpass position at the end of the sampling sequence The standard sampling sequence occurs in the following order The injection valve switches to the bypass position The plunger of the metering device moves to the initialization position The needle lock moves up The needle moves to the desired sample vial position The needle lowers into the vial The metering device draws the preset sample volume The needle lifts out of the vial The needle is then moved onto the seat to close the sample loop O oO N our won The needle lock moves down 10 The injection cycle is completed when the injection valve switches to the mainpass position If needle wash is required it will be done between step 7 and 8 Injection Sequence Before the start of the injection sequence and during an analysis the injection valve is in the mainpass position Figure 2 on page 13 In this position the mobile phase flows through the autosampler metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over 1200 Series Autosamplers Service M anual Introduction 1 Figure2 Mainpass Position When the sample sequence begins the valve unit switches to the bypass position Figure 3 on page 13 Solvent from the pump enters the valve
138. needle Unlock the needle tighter lock system Loosen the loop capillary fitting on the analytical head side Remove the loop capillary corrugated waste tube Pinch the holder clamp pull back and remove the needle assembly with the loop capillary from the needle carrier Attach the 5 16 inch wrench to hold position at the needle assembly Use the 4 mm wrench to loosen the fitting of the loop capillary Do not bend the sheet metal of the needle 1200 Series Autosamplers Service M anual Maintenance 8 Maintenance Procedures 10 Pull the loop capillary out from the needle assembly 1200 Series Autosamplers Service M anual 177 8 Maintenance Installing the Needle Assembly When Tools required Parts required 1 1 When the needle is visibly damaged When the needle is blocked Two 1 4inch 5 16 inch wrenches 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Partnumber Description G1367 87202 Needle assembly for G1367B D G1377 87201 Needle assembly for G1377A Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be careful when you remove the needle assembly gt Use the silicon safety tube supplied with every new needle 178 Push the silicon safety tube supplied in the WPS leak kit G1367 60006 and with every new needle over the needle Pull the loop capillary in the new needle assembly G1367 87202 or G137
139. ng Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuseholders must be avoided 1200 Series Autosamplers Service M anual Appendix 13 CAUTION Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided as much as possible When inevitable this should be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless anothe
140. nt 1200 Series High Performance Autosamplers amp Micro Well Plate Autosampler The manual describes the following e introduction to the sampler e site requirements and specifications e installing configuring and using the autosampler e optimizing performance e troubleshooting and diagnostics e maintenance and repair e parts and materials e information on cables e safety and legal information Agilent Technologies 2006 2008 Printed in Germany 05 08 L387 30110 G1367 90110 os Agilent Technologies
141. nt position turn on the sampler again If there is any weight on top of the sampler remove that weight turn off the sampler and then turn it on again 1200 Series Autosamplers Service M anual 155 7 Troubleshooting and Diagnostics Instrument Logbook Errors and Step by Step Repair Process The errors in the instrument logbook can be classified in 8 groups In this section you can find a general step by step troubleshooting process for each of them 156 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 1 Fan Error Main Fan or Exhaust Fan Probable cause Suggested actions 1 Make sure the fan is connected to the main board correctly Examine the fan connector for irregularities Correct if possible e Replace the defective fan main fan 3160 1017 exhaust fan 3160 4097 e Replace the main board G1367 69520 1200 Series Autosamplers Service M anual 157 7 Troubleshooting and Diagnostics 2 Initialization Error Probable cause Suggested actions 1 Upgrade the firmware revision to 4 04 14 or higher and the ChemStation revision to A 08 04 or higher e Check the sample transport connectors on the main board sample transport e Check the connector on the bottom of the sample transport unit e Check for mechanical obstruction on the sample transport X Theta Z Change sample transport unit e Change the main board G1367 69520 158 1200 Series Autosamplers Service M anual
142. nual List of Recommended Plates and Closing Mat WARNING Explosive gas mixtures There is a risk of building explosive gas mixtures in the instrument if flammable solvents are used gt Cover the plates gt Remove the plates from the sampler after turning it OFF Using the Autosampler 5 WARNING Contamination with adhesives Closing mats with adhesive can give some contamination in the system The adhesive is soluble in most of the solvents used in HPLC gt In general do not use closing mats with adhesive The sampler has no prepunch needle therefore the adhesive will clog the needle after several injections Table 31 Recommended plates and closing mat Description Rows Columns Plate height Volume pl Part Number Package 384A gilent 16 24 14 4 80 5042 1388 30 384Corning 16 24 14 4 80 No Agilent PN 384N unc 16 24 14 4 80 No Agilent PN 96Agilent 8 12 14 3 400 5042 1386 10 5042 1385 120 96Agilent conical 8 12 17 3 150 5042 8502 25 96CappedAgilent 8 12 47 1 300 5065 4402 1 96Corning 8 12 14 3 300 No Agilent PN 96CorningV 8 12 14 3 300 No Agilent PN 96DeepAgilent31mm 8 12 31 5 1000 5042 6454 50 1200 Series Autosamplers Service M anual 85 5 Using the Autosampler Table 31 Recommended plates and closing mat Description Rows Columns Plate height Volume pl Part Number Package 96DeepN unc31mm 8 12 31 5 1000 No Agilent PN 96DeepRitter41mm 8 12 41 2 800 No Agilent PN 96Greiner 8
143. o The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1200 Series Autosamplers Service M anual Maintenance Procedures Maintenance 8 The procedures described in this section can be done with the autosampler in place in the stack These procedures can be done on a more frequent basis Table 44 Procedure Simple Repair Procedures Typical Frequency Notes Exchanging the needle assembly Exchanging the needle carrier assembly Exchanging the needle seat assembly Exchanging the stator face Exchanging the rotor seal Exchanging the metering seal Exchanging the loop capillary Exchanging the peristaltic pump When the limit in the needle into seat counter in the EM F is exceeded When needle shows indication of damage or blockage When the needle carrier is defective When the limit in the needle into seat counter in the EMF is exceeded When needle seat shows indication of damage or blockage When the valve performance shows indication of leakage or wear When the limit in the injector valve switches counter in the EMF is exceeded When the valve performance shows indication of leakage or wear When autosampler reproducibility indicates seal wear When loop capillary blocked or broken When tubing broken See Removing the Needle Assembly on page 176 See Removing the Needle Carrier Assem
144. o module 5181 1516 0 5m Ig Agilent 1100 1200 module to module 5181 1519 1m lg 1200 Series Autosamplers Service M anual 297 12 Cable Identification External Contact Cable 298 5 01 10 lt 6 15 11 One end of this cable provides a 15 pin plug to be connected to Agilent 1100 1200 Series module s interface board The other end is for general purpose Agilent 1100 1200 Series Interface Board to general purposes Table 83 ConnectorG1103 61611 Color Pin Agilent Signal Name 1100 1200 a White 1 EXT 1 Brown 2 EXT1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 1200 Series Autosamplers Service M anual Cable Identification 12 RS 232 Cable Kit This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter Use the cable and adapter to connect Agilent Technologies instruments with 9 pin male RS 232 connectors to most PCs or printers Agilent RS 232 Cable Kit 34398A 1100 1200 module toPC Instrument PC OANDUARWND OANODARWND DB9 DB9 DB9 Female Female Male 1200 Series Autosamplers Service M anual 299 12 Cable Identification LAN Cables Recommended Cables Table 84 Description Part number C
145. oducts e analog output connector s for signal output and e interface slot for specific interfacing external contacts BCD LAN and so on For identification and location of the connectors see the module manual Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EM C regulations 72 1200 Series Autosamplers Service M anual Configuring the Autosampler 4 CAN The CAN is intermodule communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement If a Agilent 1100 1200 series detector DAD M W D FLD VW D RID is in the system the LAN should be connected to the DAD M W D FLD VW D RID due to higher data load If no Agilent detector detector is part of the system the LAN interface should be installed in the pump or autosampler LAN The 1100 1200 modules have either an interface slot for an LAN card e g Agilent G1369A LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a connected PC with the appropriate control software e g Agilent ChemStation GPIB The GPIB connector is used to connect the module with a computer The address and control switches next to the GPIB connector determine the GPIB address of your module The switches are preset to a default address
146. on Introduction to the 1200 Series Samplers Sampling Sequence 11 Injection Sequence 12 Sampling Unit 15 AnalyticalHead 16 Injection Valve 16 Needle Flush Station 17 Needle Lock 17 Needle Sample Transport Assembly 18 Advanced Operating Modes 20 Early Maintenance Feedback EMF 22 Electrical Connections 23 8 ee Agilent Technologies 1 Introduction Introduction to the 1200 Series Samplers Three models of Agilent 1200 Series samplers are available that handle vials well plates and Eppendorf tubes e G1367B Agilent 1200 Series high performance autosampler e G1367D Agilent 1200 Series high performance autosampler SL e G1377A Agilent 1200 Series micro well plate autosampler The G1367B Agilent 1200 Series high performance autosampler adds maximum flexibility and fast injection cycles whenever high sample throughput and speed of analysis are required Features Increased sample injection speed for high sample throughput overlapped injections for increased productivity minimal delay volumes for rapid gradients and fast equilibration when bypassing the autosampler after sample injection flexible and convenient sample handling with different types of sample containers Using 384 well plates allows to process up to 768 samples unattended The G1367D Agilent 1200 Series High Performance Autosampler SL is specifically designed for the Agilent 1200 Series Rapid Resolution LC system for increased analysis speed with uncompromised
147. on board Remote Pumps G1310A ISO 2 Yes No Yes Yes 1 Yes G1311A QUAT G1312A BIN G2226A NANO G1312B BIN SL 2 Yes No Yes Yes 1 Yes G1361A PREP 2 Yes No No Yes No Yes CAN DC OUT for CAN slaves Samplers G1313A STD 2 Yes No Yes Yes No Yes G1329A STD 2 Yes No Yes Yes No Yes THERM OSTAT for G1329B STD SL G1330A B G2260A PREP G1364A FRC 2 Yes No Yes Yes No Yes THERM OSTAT for G1367A B C D G1330A B WPS CAN DC OUT for G1377A WPS CAN slaves G2258A D LOOP Detectors G1314A B VWWD 2 Yes No Yes Yes 1 Yes G1314C VWD SL 2 Yes No No Yes 1 Yes G1315A B DAD 2 Yes No Yes Yes 2 Yes G1365A B MWD 1200 Series Autosamplers Service M anual 71 4 Configuring the Autosampler Module CAN LAN BCD LAN GPIB RS 232 Analog APG Special optional on board Remote G1315C DAD SL 2 No Yes No Yes 2 Yes G1365C MWD SL G1315D DAD G1365D MWD G1321A FLD 2 Yes No Yes Yes 1 Yes G1362A RID G4280A ELSD No No NO No Yes Yes Yes EXT Contact AUTOZERO Others G1316A TCC No No No A Yes No Yes G1316B TCC SL No No No A Yes No Yes G1322A DEG No No No No No No Yes AUX G1379A DEG No No No No Yes No No AUX G4240A CHIP CUBE 2 Yes No No Yes No Yes CAN DC OUT for CAN slaves THERM OSTAT for G1330A B NOT USED e CAN connectors as interface to other Agilent 1200 Series modules e GPIB connector as interface to the Agilent ChemStation e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent pr
148. onnected to pin 6 alla 5 Shield Analog 1 BRN 7 BD 6 Connected to pin 4 7 Key 8 Not connected 1200 Series Autosamplers Service M anual Agilent 1100 1200 to 3394 6 Integrators Cable Identification 12 Table 69 Connector35900 60750 Pin 3394 6 Pin Agilent Signal Name 1100 1200 1 Not connected 2 Shield Analog 3 Center Analog so oo 20 Agilent 1100 1200 to BNC Connector Table 70 Connector8120 1840 Pin BNC Pin Agilent Signal Name 1100 1200 Shield Shield Analog Center Center Analog 1200 Series Autosamplers Service M anual 285 12 Cable Identification Agilent 1100 1200 to General Purpose Table 71 Connector01046 60105 Pin 3394 6 Pin Agilent Signal Name 1100 1200 1 Not connected 2 Black Analog 3 Red Analog se 286 1200 Series Autosamplers Service M anual Remote Cables 0000 Cable Identification 12 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent 1100 1200 Series modules The other end depends on the instrument to be connected to Agilent 1100 1200 to 3390 Integrators Table 72 Connector01046 60203 Pin 3390 Pin Agilent SignalName Active 1100 1200 TTL 2 1 White Digital ground NC 2 Brown Prepare run Low gt 7 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected
149. ools icon 1200 Series Autosamplers Service M anual 183 8 Maintenance Maintenance Procedures NOTE The seat capillary can be exchanged alone if the needle seat is not damaged 184 1200 Series Autosamplers Service M anual Maintenance 8 Exchange the Seat Capillary on the G1377A and G1367D When Tools required Parts required PrP PrP PPP When the seat is visibly damaged When the seat capillary is blocked 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Flat screwdriver Partnumber Description G1375 87317 Seat Capillary 150 mm 0 10 mm ID for G1377 87101 Needle Seat G1375 87316 Seat Capillary 150 mm 0 075 mm ID for G1377 87101 Needle Seat G1375 87300 Seat Capillary 150 mm 0 05 mm ID for G1377 87101 Needle Seat G1377 87101 Optional Needle Seat without capillary for G1377A G1367 87303 Seat Capillary 150 mm 0 120 mm ID for G1367 87104 Needle Seat G1367 87302 Seat Capillary 150 mm 0 170 mm ID for G13767 87104 Needle Seat G1367 87105 Optional Needle Seat without capillary for G1367D Disconnect the seat capillary from the injection valve port 5 with the 1 4 5 16 inch wrench Remove the needle seat see Exchange the Needle Seat G1367 87105 on the G1367D Exchange the Needle Seat G1377 87101 on the G1377A on page 183 Use the seat capillary mounting tool provided in the accessory kit and replace the seat capi
150. ools required Parts required 250 When defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Partnumber Description 5061 3356 Leak sensor In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 Remove the plate tray from the tray base Remove the transport assembly see Removing the Sample Transport on page 209 Remove the sampling unit see Removing the Sampling Unit on page 212 Disconnect the leak sensor from the SUD board labelled leak sensor Remove the sensor Installing the Leak Sensor 1 Feed the cable of the new sensor through the base of the sampling unit to the SUD board Connect the connector to the connector labelled leak sensor 3 Install the sampling unit see Installing the Sampling Unit on page 213 Install the sample transport see Installing the Sample Transport on page 209 Install the top cover top plate and foam see Top Cover and Foam on page 204 1200 Series Autosamplers Service M anual Repairs 9
151. oop capillary for G1367B G1367 60007 Loop capillary for G1367D Loop capillary for G1377A 8ul injection volume Loop capillary for G1377A 40ul injection volume G1377 87310 G1375 87315 G1377 87300 1200 Series Autosamplers Service M anual 255 10 Parts and Materials for Maintenance Table 47 Autosampler M ain Assemblies Item Description Part Number 15 Illumination assembly for sampler G1367 60040 Sampler TCC cap 380 mm 0 17 mm id for G1367 68A 01090 87306 Sampler TCC cap 500 mm 0 05 mm id for G1377 78A G1375 87304 Fan not visible 3160 1017 Fan exhaust not visible 3160 4097 BCD board not visible G1351 68701 256 1200 Series Autosamplers Service M anual Parts and Materials for Maintenance 10 Vial Trays Vial Trays Table 48 Autosampler Vial Trays and Tray Base Item Description Part Number 1 Tray for 2 plates 10 x 2 ml vials G2258 60011 2 Screws for springs 0515 0866 3 Spring G1313 09101 4 Spring stud 0570 1574 5 Tray base includes items 4 5 6 G1329 60000 6 Adapter air channel G1329 43200 Plug channel not shown G1367 47200 Figure 32 Vial trays and Tray Base 1200 Series Autosamplers Service M anual 257 10 Parts and Materials for Maintenance Table 49 Recommended plates and closing mat Description Rows Columns Plate height Volume pl Part Number Package 384Agilent 16 24 14 4 80 5042 1388 30 384Corning 16 24 14 4 80 No Agilent PN
152. osampler is part of a system you can ensure optimum performance ensuring minimum delay volume by installing the following configuration Figure 8 on page 38 and Figure 9 on page 39 show the configuration recommended for the sampler Figure 10 on page 40 and Figure 11 on page 41 show the configuration recommended for the thermostatted sampler 1200 Series Autosamplers Service M anual 37 3 Installing the Autosampler Optimizing the Stack Configuration Solvent cabinet Vacuum degasser Instant Pilot Autosampler Column compartment Detector Figure8 Recommended Stack Configuration Well Plate Autosampler Front View 38 1200 Series Autosamplers Service M anual Installing the Autosampler Optimizing the Stack Configuration AC power Remote cable AC power CAN Bus cable AC power AC power AC power GPIB or LANto control software Figure 9 Recommended Stack Configuration Well Plate Autosampler Rear View 1200 Series Autosamplers Service M anual 3 39 3 Installing the Autosampler Optimizing the Stack Configuration Instant Pilot Detector Column Compartment Solvent Cabinet Autosampler Degasser ALS Thermostat Pump Figure 10 Recommended Stack Configuration Thermostatted Autosampler Front View 40 1200 Series Autosamplers Service M anual Installing the Autosampler 3 Optimizing the Stack Configuration GPIB or LAN Analog signal to LC ChemStation to recorder
153. out of the module 14 Remove the coupler from the switch of the power supply 1200 Series Autosamplers Service M anual 247 9 Repairs Installing the Power Supply m Insert the coupler onto the switch of the new power supply Install the power supply in the module Connect the light pipe to the coupler Install the bottom foam Ensure the cables are positioned properly Install the exhaust fan see Installing the Exhaust Fan on page 230 Install the MTP board see Installing the MTP Main Board on page 233 Install the main fan see Installing the Main Fan on page 228 Install the sampling unit see Installing the Sampling Unit on page 213 O O N ou FP WwW N Install the sample transport see Installing the Sample Transport on page 209 10 Install the top cover top plate and foam see Top Cover and Foam on page 204 11 Replace the plate tray in the tray base 12 Turn ON the sampler close the front door The system will reset automatically 13 The system will reset automatically 14 In the user interface perform a Sample Transport Self Alignment In the Agilent Lab Monitor amp Diagnostic Software the Sample Transport Self Alignment can be found under the Calibration icon 248 1200 Series Autosamplers Service M anual Repairs 9 Leak Sensor Leak Sensor 1200 Series Autosamplers Service M anual 249 9 When Repairs Removing the Leak Sensor T
154. pair Sampling Unit Assembly Sampling Unit Assembly 12 11 Figure 34 High performance autosampler High performance autosampler SL and Micro well plate sampler sampling unit 266 1200 Series Autosamplers Service M anual Table56 Autosampler sampling unit Item Description Parts for Repair Part Number 11 FP Ww N 10 11 12 13 14 Sampling Unit assembly for G1367B Sampling Unit assembly for G1367D Sampling Unit assembly for G1377A The assy comes without injection valve and analytical head Sampling unit connector board SUD Belt gear for metering unit and needle arm Stepper motor for metering unit and needle arm Loop capillary 100 ul for G1367B Loop capillary 40 ul for G1367D Loop capillary 40 ul for G1377A Loop capillary 8 ul for G1377A Loop capillary waste tube Seal tight nut for G1367 87300 and G1377 87300 capillaries Analytical Head assembly 100 ul for G1367B Analytical Head assembly 40 ul for G1367D Analytical Head assembly 40 ul for G1377A Peristaltic pump includes tubing Inj Valve Anal Head cap 160 mm 0 25 mm ID for G1367B D Inj Valve Anal Head cap 200 mm 0 10 mm ID for G1377A Leak sensor Waste tube for G1367B D Waste tube for G1377A Injection Valve assembly for G1367B Injection Valve assembly for G1367D Micro Injection Valve assembly for G1377A Seat adapter Flush port 1200 Series Autosamplers Service M anual G1367 60008 G1367 60028 G1377 60008
155. pillaries are available for the high performance autosampler the SL version and the micro well plate autosampler For the high performance autosampler The needle seat assembly includes the needle seat and the seat capillary The part number for this assembly is G1367 87101 For the high performance autosampler SL The needle seat assembly is made up of two parts Needle seat G1367 87105 Seat capillary G1367 87303 0 12 mm 150 mm G1367 87302 0 17 mm 150 mm The capillary pre installed in the high performance autosampler SL is the G1367 87302 For the micro well plate sampler The needle seat assembly is made up of two parts Needle seat G1377 87101 Seat capillary G1375 87317 100 um 150 mm G1375 87316 75 um 150 mm G1375 87300 50 um 150 mm part number G1375 87317 100 um is the capillary preinstalled in the micro well plate autosamplers upon delivery This capillary is recommended for applications with a 0 3 mm column or higher It provides less plugging of the capillary in general and especially with biological samples For small K this capillary can provide a higher peak width for isocratic analysis 1200 Series Autosamplers Service M anual 107 6 Optimizing Performance part number G1375 87316 75 pm is available as a spare part and is recommended for applications with a 0 3 mm column or smaller This capillary gives full chromatographic performance part number G1375 87300 50 pm is avai
156. plugged before disconnecting or reconnecting the sampler to thermostat cable WARNING Damage through condensation If the condensation tube is located in liquid the condensed water cannot flow out of the tube and the outlet is blocked Any further condensation will then remain in the instrument This may damage the instruments electronics gt Make sure that the condensation tube is always above the liquid level in the vessel WARNING Personal injury To avoid personal injury keep fingers away from the needle area during autosampler operation gt Do not attempt to insert or remove a vial or a plate when the needle is positioned 1200 Series Autosamplers Service M anual 45 3 Installing the Autosampler 1 Place the thermostat on the bench 2 Remove the front cover and route the condensation drain tube to the waste bottle Condensation drain tube Waste bottle J Figure 13 Condensation leak outlet Install the LAN interface board in the sampler if required Remove the adhesive tape which covers the side and front doors Open the front door and remove the left side door Remove the transport protection foam Re install the corrugated waste tube in the plastic port Re install the left side door take care of the magnet at the back O On OU FS UW Place the sampler on top of the thermostat Make sure that the sampler is correctly engaged in the thermostat locks
157. port connectors on the main board sample transport Check the connector on the bottom of the sample transport Change the needle and the seat Change the needle carrier assembly G1367 60010 Change the sample transport unit Change the main board G1367 69520 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 8 Sample Location Error Probable cause Suggested actions 1 e Check the plate configuration in the user interface e Ensure the right vials and plates are used e Perform an auto alignment e Check the connector from the needle carrier to the sample transport unit e Check the sample transport connectors on the main board sample transport e Check the connector on the bottom of the sample transport unit e Change the needle and the seat e Change the needle carrier assembly G1367 60010 Change the sample transport unit e Change the main board G1367 69520 1200 Series Autosamplers Service M anual 165 7 Troubleshooting and Diagnostics Needle Centering Over the Vial or the Well 166 The positioning of the needle is very precise You have to take no action if the needle hits in the safe area metal border Septum Safe area to hit Figure 29 Vial cap If the diameter for the safe area is approximately 1mm smaller than the diameter of the septum no corrective action is necessary Action to Take if the Needle Does Not Hit in the Safe Area V Check if the right vials
158. pparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1200 Series Autosamplers Service M anual 305 13 Appendix The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex this product is classed as a Monitoring and Control Instrumentation product s Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 306 1200 Series Autosamplers Service M anual Appendix 13 Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interferen
159. r G1367 60010 on place Install the three holding hex screws with the 2 mm hex key Plug in the flex board on the sample transport Push the silicon safety tube supplied in the WPS leak kit G1367 60006 and with every new needle over the needle Pinch the holder clamp and reinsert the needle assembly into the needle carrier Check the alignment of the needle in the needle pusher of the needle carrier by viewing from several directions to see that it is aligned in the center of the needle pusher The needle must be centered in the needle pusher as all alignment by the autosampler is calculated from the needle pusher position 7 8 9 Remove the silicon safety tube from the needle Replace the plate tray in the tray base Re install the side door and close the front door 10 In the user interface close the Change Needle Carrier function and exit the maintenance mode The instrument will reset In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Carrier function can be found in the Tools icon 1200 Series Autosamplers Service M anual 181 8 Maintenance Exchange the Needle Seat Assembly G1367 87101 on the G1367B Samplers When Tools required Parts required When the seat is visibly damaged When the seat capillary is blocked 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Flat screwdriver Partnumb
160. r person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1200 Series Autosamplers Service M anual 303 13 Appendix Safety Symbols Table 85 Safety Symbols Symbol Description A f AN The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this produ
161. r rotation motor e Motor 1 temperature Z axis motor The processor monitors the current drawn by each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the motor draws current is too long the error message is generated Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 High friction in the transport assembly Exchange the sample transport assembly 3 Motor belt tension too high Switch off the autosampler at the power switch Wait at least 10 minutes before switching on again 4 Defective motor Exchange the sample transport assembly 5 Defective transport assembly flex board Exchange the MTP main board 138 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 _ Invalid Vial Position The vial position defined in the method or sequence does not exist The reflection sensors on the transport assembly flex board are used to check automatically which sample trays are installed coding on tray If the vial position does not exist in the current sample tray configuration the error message is generated Probable cause Suggested actions 1 Incorrect tray installed Install the correct trays or edit the method or sequence accordingly 2 Incorrect tray definition Install the correct trays or
162. rd and the 8 bit configuration switch which is used to configure the address for the communication baud rate for RS 232 transfer and so on Also the ASIC controls and drives module specific functions and reads static status signals Leak Sensor Solvent leaking from the autosampler cools down the PTC This changes the resistance of the PTC and generates a leak signal 1200 Series Autosamplers Service M anual Configuring the Autosampler 4 Fan Drive The fan speed two speeds are possible is controlled by the main processor according to the internal heat distribution inside the module The fan provides a PWM signal which is proportional to the revolution Onboard Battery An onboard lithium battery buffers the electronic memories when the module is turned off For safety information on lithium batteries see Lithium Batteries Information on page 305 Autosampler Specific Electronics The autosampler specific functions provided by the electronics are e Closed loop control of three axis vial handling servos e Electric valve control e Needle unit control e Metering device control e Peristaltic pump control Transport Unit Control The transport drive electronics use current controlled pulse width modulation PWM to drive the X Z motors in closed loop servo control mode Dedicated electronics in the ST L6506 provide the current control loop Commutation is done in FPGA logic The ST L6201 SMT output drivers a
163. re used for all three stepper motors Motor encoder signals are connected to the ASIC where the encoder quadrature decoded clock and the up down signal are used in the FPGA to provide instantaneous stepper motor commutation with respect to the motor rotor position Wiring between the autosampler main board ASM and the motors and encoders uses a flat band cable 64 pin and a flex board on which 13 reflection light sensors are located 9 light sensors are used for vial tray identification and four for decoding of the initialization position 1200 Series Autosamplers Service M anual 59 4 60 Configuring the Autosampler Theta Drive Motor 6A phase Encoder FPGA X Axis Drive Motor i Via transport 6A phase Encoder unit flex board Z Axis Drive Motor 6A phase Encoder ASIC Reflection sensors on transport unit flex board Tray decoding 9 initialization sensors 4 Figure 18 Transport unit control Sampling Unit Control Needle lock metering device valve and peristaltic pump motors are driven by controlled pulse width modulation in the same way as the ST L6506 see Transport Unit Control on page 59 The motors require fast speed but do not require precise position control Therefore a closed loop servo system is not required Commutation is done in FPGA logic The needle lock metering device and valve motors use ST L6203 output drivers to deliver the higher currents required for fast movement or high torqu
164. rial for the carrier s inspection Delivery Checklist Ensure all parts and materials have been delivered with the autosampler For this compare the shipment content with the checklist included in each instrument box Please report missing or damaged parts to your local Agilent Technologies sales and service office Accessory Kits Each shipment contents an Accessory kit with the necessary tools to install the system and to have an operating system e The Accessory kit G1367 68705 shown in Table 7 on page 35 is shipped with the G1367B D high performance autosampler and SL version e The Accessory kit G1377 68705 shown in Table 8 on page 35 is shipped with the G1377A micro well plate autosampler 34 1200 Series Autosamplers Service M anual Installing the Autosampler 3 Table 7 High Performance Autosampler and SL version Accessory Kit Contents G1367 68705 Description Quantity Part Number Capillary sampler column 380 mm 0 17 mm ID 1 01090 87306 96 well plate 0 5 ml PP pack of 10 1 5042 1386 Tubing assembly 1 5063 6527 Filter kit 1 5064 8240 CAN cable 1 m 1 5181 1519 Vials screw cap 100 pk 1 5182 0716 Blue screw caps 100 pk 1 5182 0717 Hex key 9 64 inch for injection valve screws 1 8710 0060 Wrench 4mm both ends 2 8710 1534 Rheotool socket wrench 1 4 inch 1 8710 2391 Hex key 4 0 mm 15 cm long T handle 1 8710 2392 Hex key 9 64 inch 15 cm long T handle 1 8710 2394 Hex key 2 0 mm 1 8710 2438 Air channe
165. rms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 J une 1995 oras a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 J une 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 J une 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 J une 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the in
166. ror messages arm movement 129 compensation sensoropen 123 compensation sensor short 124 fan failed 125 front door error 127 initialization failed 136 invalid vial position 139 leaksensoopen 121 leak sensor short 122 1200 Series Autosamplers Service M anual Index leak 120 metering home failed 137 missing vial 135 motor temperature 138 needle lock failed 132 needle to needle seat position 133 peristaltic pump error 140 shut down 117 synchronization lost 118 timeout 116 valve to bypass failed 130 valve to mainpass failed 131 vessel error 141 vessel stuck to needle 142 ESD electrostatic discharge strap 169 external contact cable 283 298 F factory settings 77 failure 111 fan failed 125 firmware description 63 main system 63 resident system 63 update tool 64 updates 63 flow connections 49 flow cell 309 solvent information 309 Forced Cold Start settings 80 fuses BCD board 66 G G1373A 262 GPIB cable 283 GPIB default addresses 78 interface 73 H hallsensor 57 HP J etDirect card 69 illumination assembly 206 information on lithium batteries 305 injection valve 16 injection volume precision 104 injection volumes less than 2 yl 104 injection sequence 12 injection valve 8 15 injection volume 104 injection volumes 104 installing the autosampler flow connections 49 interface cables 42 powercable 42 safety 42 sample trays 84 installing the sample tray 51 installing the thermostatted autosampler in
167. ross over network cable shielded 3 m long for point to 5023 0203 point connection Twisted pair network cable shielded 7 m long for hub 5023 0202 connections 300 1200 Series Autosamplers Service M anual 1200 Series Autosamplers Service M anual e e 13 Appendix General Safety Information 302 Lithium Batteries Information 305 The Waste Electrical and Electronic Equipment Directive 306 Radio Interference 307 Sound Emission 308 Solvent Information 309 Agilent Technologies on the Internet 311 This appendix provides general safety and environmental information gg Agilent Technologies 301 13 Appendix General Safety Information General Safety Information General The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Aligent Technologies assumes no liability for the customer s failure to comply with these requirements This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation 302 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operati
168. rs Service M anual 1200 Series Autosamplers Service M anual e 9 Repairs Exchanging Internal Parts 199 Warnings and Cautions 200 Assembling the Main Cover 202 Light Protection Kit Installation 203 Top Cover and Foam 204 Removing the Top Cover and Foam 205 Installing the Top Cover and Foam 205 Illumination Assembly 206 Transport Assembly 208 Removing the Sample Transport 209 Installing the Sample Transport 209 Sampling Unit 211 Removing the Sampling Unit 212 Installing the Sampling Unit 213 Analytical Head 214 Removing the Analytical Head 215 Installing the Analytical Head 215 Peristaltic Pump Motor 216 Removing the Peristaltic Pump Motor 217 Installing the Peristaltic Pump Motor 217 Injection Valve Assembly 219 Removing the Injection Valve Assembly 220 Installing the Injection Valve Assembly 220 Metering Drive Motor and Belt 222 Removing the Metering Drive Motor and Belt 223 Installing the M etering Drive Motor and Belt 223 Needle lock M otor and Belt 225 Removing the Needle Lock M otor and Belt 226 Ee Agilent Technologies 197 9 Repairs Installing the Needle Lock Motorand Belt 226 Main Fan 227 Removing the Main Fan 228 Installing the Main Fan 228 Exhaust Fan 229 Removing the Exhaust Fan 230 Installing the Exhaust Fan 230 MTP Main Board 232 Removing the MTP Main Board 233 Installing the M TP Main Board 233 Introduction 235 SUD Board 240 Removing the SUD Board 241 Installing the SUD Board 241
169. rs the autosampler through a recess underneath the special designed sample tray The air is then distributed evenly through the sample tray ensuring effective temperature control regardless of how many vials are in the tray In cooling mode condensation is generated on the cooled side of the Peltier elements This condensed water is safely guided into a waste bottle for condensed water 1200 Series Autosamplers Service M anual Introduction 1 Sampling Sequence Sampling Sequence Sample transport MTP board Loop capillary Illumination Kit Sampling unit Power supply Analytical head Peristaltic pump Vial tray Switching valve Needle Needle seat Figure1 Overview of the autosampler The movements of the autosampler components during the sampling sequence are monitored continuously by the autosampler processor The processor defines specific time windows and mechanical ranges for each movement If a specific step of the sampling sequence is not completed successfully an error message is generated Solvent is bypassed from the autosampler by the injection valve during the sampling sequence The needle moves to the desired sample vial position and is lowered into the sample liquid in the vial to allow the metering device to draw up the desired volume by moving its plunger back 1200 Series Autosamplers Service M anual 11 1 12 Introduction a certain distance The needle is then raised again a
170. sampler In the Agilent Lab Monitor amp Diagnostic Software the maintenance positions can be selected from the Tools icon Maintenance Functions The maintenance functions moves the arm assembly in a specific position in order to enables easy access for maintenance operations Home Position The home position function moves the arm to the right side for better access and exchange of the trays Park Position The park position function moves the arm to the left side of the tray In this position it is possible to secure the sample transport mechanism with the protection foam The sample transport is than ready for transporting Change Piston The change piston function draws the piston away from the home position relieving the tension on the spring In this position the analytical head assembly can be removed and reinstalled easily after maintenance This position is also used to change the analytical head plunger and metering seal 1200 Series Autosamplers Service M anual 143 7 Troubleshooting and Diagnostics Table 41 Maintenance positions Function Arm position in X Arm position in Theta Arm Position in Z Note Change Needle Change Carrier assembly Change Loop capillary Home position Park arm Left side Straight Up No current on Theta Left side Straight Middle No current on the ST Middle Left Up Right side Left rear Up Left side Right rear Up Sample Transport Self Alignment 1
171. sampling arm on the well plate Once the sampling arm is positioned over the programmed sample position the programmed sample volume is drawn by the metering device into the sampling needle The sampling arm then moves to the injection position where the sample is flushed onto the column The autosamplers employ a vial plate pusher mechanism to hold down the vial or the plate while the needle is drawn back from the sample vessel a must in the case a septum is used This vial plate pusher employs a sensor to detect the presence of a plate and to ensure accurate movement regardless of plate used All axes of the transport mechanism x z theta robot are driven by stepper motors Optical encoders ensure the correct operation of the movement The standard metering device for the G1367B provides injection volumes from 0 1 100 ul A multi draw kit extends the range up to 1500 ul A 0 1 40u1 injection volume metering device is installed in the G1367D with the option to install an extended volume metering device and loop capillary with up to 100ul injection volume The micro metering device for the G1377A provides injection volumes from 0 01 8 ul with the standard loop capillary installed and from 0 01 40 ul with the extended loop capillary The entire flowpath including the metering device is always flushed by the mobile phase after injection for minimum internal carry over An additional needle flush station with a peristaltic pump is instal
172. se features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal level are defined as e standard TTL levels 0 V is logic true 5 V is false e fan out is 10 e input load is 2 2 kOhm against 5 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 volt power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 V with respect to the ground terminal Table 20 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration de
173. sory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Flat head screwdriver if interface board installed Partnumber Description G1367 60019 Sample Transport assembly for the G1367B D G1377 60009 Sample Transport assembly for the G1377A In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 Remove the loop capillary corrugated waste tube 5 Disconnect the loop capillary from the analytical head Use a 1 4 inch wrench if you remove a SST capillary or a 4 mm wrench if you remove a fused silica capillary Lift out the transport assembly This may require a flat head screwdriver to separate the sample transport assembly from the sampling unit Installing the Sample Transport Slide the new transport assembly into the autosampler Ensure the transport assembly is seated firmly Reconnect the loop capillary to the analytical head Use a 1 4 inch wrench if you reconnect a SST capillary or a 4 mm wrench if you reconnect a fused silica capillary Reinstall the loop capillary corrugated waste tube 1200 Series Autosamplers Service M anual 209 9 Repairs 5 Install
174. suitable wash solvent suitable mobile phase column packing material e g interaction of basic sample with silanols of stationary phase The autosampler continuous flow through design ensures that sample loop needle inside seat capillary and the mainpass of the injection valve is always in the flow line These parts are continuously flushed during an isocratic and also during a gradient analysis The residual amount of sample remaining on the outside of the needle after injection may contribute to carry over in some instances When using small injection volumes or when injecting samples of 1200 Series Autosamplers Service M anual 95 6 96 Optimizing Performance low concentration immediately after samples of high concentration carry over may become noticeable Cleaning the needle in the flush port or using the automated needle wash enables the carry over to be minimized and prevents also contamination of the needle seat Module dependant carry over performance All Agilent 1200 series High Performance Autosampler are considering low carry over Nevertheless the Agilent 1200 series High Performance Autosampler SL is best performing regarding carry over General recommendations for lowest carry over Issues in setting up the experiments Use a flush solvent which dissolves the sample well e g aqueous organic acidic for basic samples set needle wash time to at least 10 sec Use a sample solvent which is compatible with s
175. t The position of the needle is monitored by a position encoder on the needle carrier If the needle fails to reach the end point or if the encoder fails to recognize the needle carrier movement the error message is generated Probable cause Suggested actions 1 Bad sample transport sampling unit Do an auto alignment alignment 2 Bent needle Check and exchange the needle assembly if necessary 3 Missing needle Clean or change the needle seat assembly if necessary 4 Blocked seat Exchange the needle carrier assembly 5 Defective position sensor in the needle Exchange the sample transport assembly carrier assembly 6 Defective MTP board Exchange the MTP board 1200 Series Autosamplers Service M anual 133 7 134 Troubleshooting and Diagnostics Needle Carrier Failed The needle carrier on the Sample Transport Assembly failed to move correctly Probable cause 1 2 3 4 5 Defective Z motor Vial pusher blocked Bad needle carrier positioning in X or Theta Defective vial pusher sensor Defective MTP main board Suggested actions Exchange the needle carrier assembly Exchange the sample transport assembly Exchange the needle carrier assembly Exchange the sample transport assembly Exchange the MTP main board 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Missing Vial or Missing Wash Vial No vial was found in the position defined in the method or sequence Wh
176. tector lamp on Receiver is any module performing preanalysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 1200 Series Autosamplers Service M anual 75 4 76 Configuring the Autosampler Table 20 Remote Signal Distribution Pin Signal Description 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces Some 1100 1200 modules have module specific interfaces connectors They are described in the module documentation 1200 Series Autosamplers Service M anual Configuring the Autosampler Setting the 8 bit Configuration Switch The 8 bit configuration switch is located next to the GPIB connector Switch settings provide configuration parameters for GPIB address serial communication protocol and instrument spec
177. tegrator 03396 60560 General purpose Spade Lugs G1351 81600 Auxiliary Agilent 1100 Series vacuum degasser G1322 61600 282 1200 Series Autosamplers Service M anual Cable Identification 12 Type Description Part Number CAN cables Agilent 1100 1200 module to module 0 5m Ig 5181 1516 Agilent 1100 1200 module to module 1m lg 5181 1519 External Agilent 1100 1200 Series interface board to general G1103 61611 contacts purpose GPIB cable Agilent 1100 1200 module to ChemStation 1 m 10833A Agilent 1100 1200 module to ChemStation 2 m 10833B RS 232 cable Agilent 1100 1200 module to a computer 34398A This kit contains a 9 pin female to 9 pin female Null M odem printer cable and one adapter LAN cable Twisted pair cross over LAN cable shielded 3m long for 5023 0203 point to point connection Twisted pair cross over LAN cable shielded 7m long for 5023 0202 point to point connection 1200 Series Autosamplers Service M anual 283 12 Cable Identification Analog Cables 284 One end of these cables provides a BNC connector to be connected to Agilent 1100 1200 Series modules The other end depends on the instrument to which connection is being made Agilent 1100 1200 to 3390 2 3 Integrators Table 68 Connector01040 60101 Pin 3390 2 3 Pin Agilent Signal Name 1100 1200 1 Shield Ground 2 Not connected 8 7 3 Center Signal 6 J RD 4 C
178. ter acetonitrile The life time of the tubing in the peristaltic pump is shortened by the usage of organic solvents Cleaning the needle seat If flush port has run out of solvent or the option needle wash hasn t been used for several injections or in case the needle seat has got contaminated the needle seat may be contaminated and carry over is significantly higher than expected For cleaning the needle seat there is an automatic procedure using the mobile phase s solvents If that doesn t work a manual cleaning has to be done the following procedure can be used to clean the needle seat Automatic procedure There s an injector command for flushing the seat So a cleaning method can be set up using an injector program Injector program e INJECT e FLUSH SEAT for 90 0 sec 0 0 mm offset e VALVE mainpass Line 1 starts the run so the pump s time table is started Line 2 let s the needle move above the seat and switches the valve to mainpass so that the pump s solvent is directed through the loop and the needle onto the seat The liquid 1200 Series Autosamplers Service M anual 99 6 100 Optimizing Performance leaves the seat via the drainage for the flush port The offset can be used for getting a kind of blast pipe effect Generally an offset of 0 0mm is a proper value After the flush time here 90sec the valve is switched to bypass Line 3 moves the needle back into the seat and switches valves back to m
179. terface cables 45 powercable 45 preparation 46 Safety 42 45 installing the thermostatted autosampler tray cover and front cover 53 Instant Pilot changing serial number and type 237 instrument status indicator 113 interface board BCD LAN 66 interface Agilent 1200 Series 71 1200 Series Autosamplers Service M anual analog signal output 74 CAN 73 GPIB 73 remote 75 RS 232C 73 interfaces special 76 internet 311 311 introduction to the autosampler 8 IRsensor 57 L LAN cables 300 LAN communication interface board 69 interface board 66 leaksensoropen 121 leak sensor short 122 leak 120 LM D software changing serial number and type 235 low volume injections 104 M mainboard 58 mainpass 12 maintenance functions 111 143 maintenance procedures 175 maintenance functions 172 step commands 145 maintenance procedures 22 metering device 104 metering device 15 metering seal 269 microswitches 57 missing parts 34 MTP board 58 multi draw option 8 313 Index needle flush station 17 needle lock 17 needle drive 15 needle sample transport assembly 18 numbering of vials 84 0 operating modes 20 optimizing performance automated needle wash 104 delay volume 104 delay volume adjustment 104 early maintenance 104 inject valve seal 104 low volume capillary kit 104 P park transport assembly 53 parts and materials cover parts 275 275 foam parts 276 leak system parts 279 power and st
180. the Autosampler l F E De Arb H gt J gt 4 lt 4 C gt Figure 27 Well Plate Dimensions staggered Table 39 Well Plate Dimensions Location Description Definition Limits Rows Number of rows on the plate up to 16 Columns Number of columns on the plate up to 24 Volume Volume in ul of a sample vessel A Row distance Distance in mm between the center of two rows B Column distance Distance in mm between the center of two columns C Plate length X size in mm at the bottom ofthe 127 75 0 25 mm plate SBS Standard 90 1200 Series Autosamplers Service M anual Table 39 Well Plate Dimensions Location Description Using the Autosampler 5 Definition Limits D Plate width E Plate height F Row offset G Column offset H Column shift Well diameter J Well depth Y size in mm at the bottom of the plate Size in mm from the bottom to the top of the plate Distance in mm from the back edge bottom to the center of the first hole A1 Distance in mm from the left edge bottom to the center of the first hole A1 Offset in mm to Y when the rows are not straight but staggered Diameter in mm of the well Distance in mm from the top of the plate to the bottom of the well 85 50 0 25 mm SBS Standard up to 47 mm at least 4mm up to 45mm The distances need to be measured with high precision It is recommended to use calipers 1200 Series Autosamplers Service M anual
181. the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used CAUTION Electrostatic discharge at electronic boards and components Electronic boards and components are sensitive to electrostatic discharge ESD gt In order to prevent damage always use an ESD protection when handling electronic boards and components Using the ESD Strap 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 30 Using the ESD Strap 1200 Series Autosamplers Service M anual 169 8 Maintenance Cleaning the module The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth as liquid may drip into the module WARNING Liquid shall not touch the module electronics It could cause shock hazard and it could damage the module gt Do not let liquid drip into the module 170 1200 Series Autosamplers Service M anual Maintenance 8 Overview of M ain Repair Procedures Overview of M ain Repair Procedures MTP board Transport assembly Illumination Kit Loop capillary SLS board Power supply Analytical head
182. the problem of discriminating sample compounds Contraindication for usage e If run times are below 2 3min the purge kit won t help as the purge step with reasonable purge volume lasts at least 2min e In highly carry over sensitive applications purge kit is not recommended as best performance is got when the Autosampler stays in mainpass the full solvent gradient 1200 Series Autosamplers Service M anual 97 6 98 Optimizing Performance Control software support for the Purge kit is provided and fully operated and controlled through the ChemStation or and G4208A Instant Pilot via the Autosampler Settings Requires Agilent ChemStation B 01 03 or higher and Firmware A 06 01 and higher on ALL Agilent 1200 Series modules that are part of the system and Firmware B 04 01 for the Control Module Using the Automated Needle Wash The automated needle wash can be programmed either as injection with needle wash or the needle wash can be included into the injector program When the automated needle wash is used the needle is moved into the wash port after the sample is drawn By washing the needle after drawing a sample the sample is removed from the outer surface of the needle immediately As the flush port is automatically refilled with fresh wash solvent this option should be used routinely Using wash vials is usually not necessary but available for special applications Using a wash vial If a wash vial is used it should be
183. the top cover top plate and foam see Top Cover and Foam on page 204 6 Turn ON the sampler close the front door The system will reset automatically 7 In the user interface perform a Sample Transport Self Alignment In the Agilent Lab Monitor amp Diagnostic Software the Sample Transport Self Alignment can be found under the Calibration icon 210 1200 Series Autosamplers Service M anual Repairs 9 Sampling Unit Sampling Unit 1200 Series Autosamplers Service M anual 211 9 Repairs Removing the Sampling Unit When Tools required Parts required 212 9 When sampling unit defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Flat head screwdriver if interface board installed Partnumber Description G1367 60008 Sampling unit for the G1367B G1367 60028 Sampling unit for the G1367D G1377 60008 Sampling unit for the G1377A The assy comes without injection valve and analytical head In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 Remove the plate tray
184. ty data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used CAUTION Electrostatic discharge at electronic boards and components Electronic boards and components are sensitive to electrostatic discharge ESD gt In order to prevent damage always use an ESD protection when handling electronic boards and components 200 1200 Series Autosamplers Service M anual Repairs 9 Warnings and Cautions NOTE The electronics of the sampler will not allow operation when the top cover and the top foam are removed A safety light switch on the main board will inhibit the operation of the sampler Always operate the sampler with the top foam and top covers in place 1200 Series Autosamplers Service M anual 201 9 Repairs Assembling the Main Cover Parts required Partnumber Description 1 5067 1556 Cabinet kit 1 5042 8901 Name plate CAUTION Incorrect assembly of main cover The main cover cannot be disassembled once assembled incorrectly gt Observe the assembly instructions carefully The cabinet kit contains all parts but it is not assembled 1 Insert the Agilent Technologies 1200 Series name plate into the recess in the top cover Place the top cover on the bench Press the door hinge in place Press the side panels into the slots in the top cover uF W N Press the front door into the slots in the top cover 202 1200 Series Autosamplers Service M anual
185. uired Parts required When defective 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Pozidrive screwdriver Partnumber Description 3160 1017 Fan In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon Switch off the autosampler at the main power switch Remove the power cable Remove the top cover top plate and foam see Top Cover and Foam on page 204 Remove the plate tray from the tray base Remove the transport assembly see Removing the Sample Transport on page 209 Disconnect the fan connector from the MTP main board Lift the fan and carefully pull the fan out of the foam Installing the Main Fan 228 Install the new fan and connect the fan connector to the MTP main board Install the sample transport see Installing the Sample Transport on page 209 Install the top cover top plate and foam see Top Cover and Foam on page 204 4 Replace the plate tray in the tray base Turn ON the sampler close the front door The system will reset automatically The system will reset automatically 1200 Series Autosamplers Service M anual Repairs 9 Exhaust Fan Exhaust Fan 1200 Series Autosamplers Service M anual 229 9 Repairs
186. urs which requires attention before the analysis can be continued for example repair exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the instrument log book This section explains the autosampler error messages and provides information on probable causes and suggested actions to recover from error conditions 1200 Series Autosamplers Service M anual 115 7 116 Troubleshooting and Diagnostics Timeout The timeout threshold was exceeded Probable cause 1 The analysis was completed successfully and the timeout function switched off the autosampler as requested 2 A not ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Suggested actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Shut Down An external instrument has generated a shut down signal on the remote line The autosampler continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause Suggested actions 1 Leak
187. ve Assembly 219 Metering Drive M otor and Belt 222 Needle lock M otor and Belt 225 MainFan 227 Exhaust Fan 229 MTP Main Board 232 Introduction 235 SUD Board 240 SLS Board 243 Power Supply 246 Leak Sensor 249 10 Parts and Materials for Maintenance 253 Sampler Main Assemblies 254 Vial Trays 257 High Performance Autosampler and SL Version Accessory Kit G1367 68705 209 Micro Well Plate Autosampler Accessory Kit G1377 68705 260 M ulti Draw Kit G1313 68711 Only For G1367B 261 G1373A Injector Purge Kit Parts 262 Thermostat for ALS FC Spotter 264 11 Parts for Repair 265 Sampling UnitAssembly 266 Analytical Head Assembly 269 Injection Valve Assembly 272 Sheet Metal Kit 274 Cover Parts 275 1200 Series Autosamplers Service M anual Contents Foam Parts 276 Power and Status Light Pipes 278 Leak System Parts 279 12 Cable Identification 281 Cable Overview 282 Analog Cables 284 Remote Cables 287 BCD Cables 294 Auxiliary Cable 296 CAN Cable 297 External Contact Cable 298 RS 232 Cable Kit 299 LAN Cables 300 13 Appendix 301 General Safety Information 302 Lithium Batteries Information 305 The Waste Electrical and Electronic Equipment Directive 306 Radio Interference 307 SoundEmission 308 Solvent Information 309 Agilent Technologies on the Internet 311 6 1200 Series Autosamplers Service M anual 1200 Series Autosamplers Service M anual 1 Introducti
188. wer Figure 15 Connection at the rear of thermostatted Autosampler The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The sampler is turned OFF when the line power switch is protruding and the green light is OFF 48 1200 Series Autosamplers Service M anual Installing the Autosampler 3 Flow Connections to the Sampler Parts required Preparations Description 1 Parts from the accessory kits see Accessory Kits on page 34 e Sampler is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1200 Series Autosamplers Service M anual 1 Connect the pump outlet capillary to port 1 of the injection valve 2 Connect column compartment inlet capillary to port 6 of the injection valve 3 Connect the corrugated waste tube to the seat adapter and the solvent waste from the leak plane 4 Ensure that the waste tube is positioned inside the leak channel 5 Drive the tube from the peristaltic flush pump to the solvent bottle in the solvent cabinet 6 Seat capillary see recommendations in
189. yellow red 4 Line power switch with a i green light Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON Instrument Status Indicator The instrument status indicator indicates one of four possible instrument conditions 1200 Series Autosamplers Service M anual 113 7 114 Troubleshooting and Diagnostics When the status indicator is OFF and power switch light is on the instrument is in a prerun condition and is ready to begin an analysis A green status indicator indicates the instrument is performing an analysis run mode A yellow status indicator indicates a not ready condition The instrument is in a not ready state when it is waiting for a specific condition to be reached or completed for example front door not closed or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the instrument has detected an internal problem which affects correct operation of the instrument Usually an error condition requires attention for example leak defective internal components An error condition always interrupts the analysis 1200 Series Autosamplers Service M anual Troubleshooting and Diagnostics 7 Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occ
190. yping specific commands on the command line at the bottom of the main user interface screen Turn the module on Start the Agilent ChemStation 1 To enter a module serial number type the following command into the command line print sendmodules Lxxx ser YYNNNNNNNN or print sendmodules Nxxx ser YYNNNNNNNN where xxx is the module type YY is country code in capital letters and NNNNNNNN the 8 character serial number of the module in question Table 45 ChemStation Command Format Serial Number Change Modules with or without optional interface board Modules with LAN on board PRINT SEN DM ODULE Lxxx SER YYNNNNNNNN PRINT SEN DM ODULES N xxx SER YYNNNNNNNN module identifier L serial number embedded in single quotes module identifier N serial number embedded in single quotes PMP ALS THM TCC VW D DAD MWD FLD RID DAD MWD VWD 236 1200 Series Autosamplers Service M anual Repairs 9 The first two characters are letters which should be capitalized The reply line will respond with RA 0000 SER followed by the module serial number you just entered 2 To change the type of the module use the following command print sendmodules 1Lxxx TYPE XXXXX or print sendmodules Nxxx TYPE XXXXX where xxx is the module type and XXXXxX is the 5 character product number of the module e g G1314B Table 46 ChemStation Command Format Type Change Modules

Agilent 1200 Series High Performance Autosamplers & Micro Well

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Agilent 1200 Series High Performance Autosamplers &amp; Micro Well

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Agilent 1200 Series High Performance Autosamplers & Micro Well