Mo2-FITC
Contents
1. 18 10 000 L PBS 10 000 19 1 500xL Ro 1 5 L 1 200 ux L PBS BSA 0 196 NBS 29 0 1962. 9 10 11 12
3. BSA NBS 1 1 2 1 6603152 300 PBS 24mL 1mL 2 1L 8 26g 1 0g EDTA4 2 37mg pH7 2 gt 7 4 1 1 2 30 2
4. 5 10x10 mm 1 100pL 2 Ficoll paque 1 4mL PBS 2 Ficol paque 4mL 1 3 400 xg 30 4 Ficoll paque 5 PBS 4 400xg 8 6 PBS 4 C 400 xg 4 7 PBS 4C 400xg 3 8 PBS 1x10 gL 1x 10 mL 9 Fc
5. 10 15 20 30 2 PBS 8 09 L 2 12 2 9g L 1 1 0 2gL 0 2g L pH7 2 7 4 BSA 0 19 0 19 6 3 MslgM FITC 6602434 100 Propidium lodide Calbiochem 53705 0 01mg mL 0 05mg mL a Acridine orange Baker A366 3 0 005mg mL 6 Ficoll paque Pharmacia 17 0840 03 1 EDTA
6. 1 1 2 29 10 3 4 2 1 Mo2 FITC Ficoll paque 200L PBS 109 6 20wL 2 1 3 CD14 x 100
7. Fc 1 29
8. FS 9 SS 1 4 1 EDTA 6 2 90 85 Ficoll paque Ficoll paque
9. Mo2 FITC y 1 03X 0 35 r 0 95 n 50 1
10. 58 849 CD14 MY4 116 Mo2 BALB cJ P3 NS1 1 AG4 1 lg lgM H z L Mo2 FITC Fluorescein lsothyocyanate FITC Protein 20 lt 30 468 509nm 504 gt 541nm 1 0 296 0 01M 0 15M 0 19 6 CD14 488nm FITC
11. 5 5 6 7 Ficoll paque 8
12. 13 14 Mo2 CD14 MY4 CD14 T B CD2 CD3 CD5 CD19 CD21 15 CD14 GPI GPI PNH CD14 16 Ficol paque AB Fc BSA NBS PBS 17 CD14
13. 2 2 8 C 20 25C Mo2 FITC 6602507 100 0 5mL 1 10 11 12 Bernard A Boumsell L Dausset J Milstein C Schlossman SF Leukocyte Typing 1984 Berlin Springer Verlag 108 407 McMichael AJ Leukocyte Typing TL White Cell Differentiation Antigens 1987 Oxford Oxford University Press 586 589 Todd RF van Agthoven A Schlossman SF Terhorst C 1982 Structural analysis of differentiation antigens Mo1 and Mo2 on human monocytes Hybridoma 1 329 337 Todd RF Nadler LM Schlossman SF 1981 Antigens on human monocytes ident
14. AML M1 M2 3496 Mo2 AML M3 M6 ALL Mo2 Mo2 CD14 CD14 Mo2 FITC 959 Mo2 FITC 5 Mo2 FITC CD14
15. 3 10 x 10 L 10 x 103 uxL 3X10 uxL PBS 15 25 C 6 a gt 10 x 10 L 10 20 x10 2 20 30 x10 3 2 4 30 40 x10 5 40 60 x103 6 60 100 x103 10 100 200 x10 20 37 C PBS b 3 x 10 L 1 25C 500 x g 1 700rpm 5 2 3 4 LH 700
16. 10 15 AB 196 VV 10 a b 90 a 11 1 x 10 1x10 L 1mL 12 PBS 4 C 400 x g 4 13 0 05mg mL Propidium lodide 3 1 14 PBS 4C 400 xg 4 15 14 16 859 b 11 25 000 1x 10 wL 25L
17. 6602507 2009 11 2007 2 20900AMY00140000 877429 Mo2 FITC 1 IN ww CD14 55KD CD14
18. 12mm x 75mm 2 Ficol paque 1 x 10 1x10 L 1mL 3 4 C 400 xg 4 4 200L IgM MslgM FITC 5 4 C 30 6 PBS 1mL 4 C 400 xg 4 7 6 2 8 PBS EPICS Cytomics 3 Ficoll paque 1 1 x 10 200L Ficoll paque 200 L
19. 5 a b a 2mL 5 15 b PBS 400 x g 5 1mL 30 2 250L 6 PBS 7 4 C 400 xg 5 8 9 PBS 10 EPICS Cytomics 2 Ficol paque 1
20. 96 2 4 C 400xg 5 3 4 5 200L IgM MslgM FITC 6 4 C 3830 7 4 C 400 xg 5 8 3 4 9 PBS200L 4 C 400 xg 5 3 4 PBS 200L EPICS Cytomics
21. FAB TCR lgH T non T Mo2 FITC CD14 CD14 55kDa LPS LPB Mo2 FITC CD14 FAB AML M4 M5 45 1009 6 Mo2
22. 12 Propidium lodide 0 01mg mL 10 x L 13 30 Acridine orange 0 005mg mL 10L 3 14 15 100 85 1 1 12mm x 75mm 2 100L 3 200L lgM MslgM FITC 4 45
23. 15 16 17 isoenzyme _analysis Cancer Immunol Immunother 21 188 192 Buckley PJ 1991 Phenotypic subpopulations of macrophages and dendrftic cells in human spleen Scanning Microsc 5 147 158 Goyet SM Ferrero E Rettig WJ Yenamandra AK Obata F Le Beau MM 1988 The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors Science 239 497 500 Medeiros LJ Herrington RD Gonzalez CL Jaffe ES Cossman J 1991 My4 antibody staining of non Hodgkin s Lymphomas Am J Clin Pathol 95 363 368 Tasies D Montserrat E Reverter JC Villamor N Rovira M Rozman C 1995 Myelomonocytic antigens in B cell chronic lymphocytic leukemia Leuk Res 19 841 848 Lohmeyer J Hadam M Ho A Hesse A Pralle H 1987 T cell prolymphocytic leukemia T PLL with unique surface phenotype Blut 54 223 229 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC ae COULTER 6602507_0911
24. ified by monoclonal antibodies J Immunol 126 1435 1442 Todd RF Schlossman SF 1982 Analysis of antigenic determinants on human monocytes and macrophadeSs Blood 59 775 786 Todd RF Griffin JD Ritz BJ Nadler LM Abrams T Schlossman SF 1981 Expression of normal monocyte macropfage differentiation antigens on HL60 promyelocytes undergoing difterentiation induced by leukocyte conditioned medium of phorbol diester Leukemia Res 5 491 495 Nadler LM Ritz J Griffin JD Todd RF Reinherz EL Schlossman SF 1981 Diagnosis and treatment of human leukemias and Ilymphomas utilizing monoclonal antibodies In Progress in Hematology XIll E B Brown ed New York Grune and Stratton 187 225 Foon KA Todd RF 1986 Immunologic classification of leukemia and lymphoma Blood 68 1 31 Foon KA Gale RF Todd RF 1986 Recent advance in the immunologic classification of leukemia Sem Hematol 23 257 283 Griffin JD Davis R Nelson DA Davey FR Mayer RJ Schiffer C Mclntyre OR Bloomfield CD 1986 Use of surface marker analysis to predict outcome of adult acute myeloblastic leukemia Blood 68 1232 1241 Drexler HG Minowada J 1986 The use of monoclonal antibodies for the identification and classification of acute myeloid leukemias Leukemia Res 10 279 290 Drexler HG Menon M Gaedicke G Minowada J 1986 Reactivity patterns of monoclonal antibodies positive on myeloblastic leukemia cells sa defined by esterase 4 4 13 14
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