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Mouse Feeder Cell Quantification Kit

1. 7 PCR x PCR Thermal Cycler Dice Real Time System VII Appendix Hold Cycle 1 95 C 30 2 step PCR Cycle 40 95 5 60 30 FAM 8 http www takara bio co jp 7 RR290 DNA FAM DNA 1 2 3
2. mtDNA Vil Appendix Thermal Cycler Dice Real Time System Thermal Cycler Dice Real Time System 1 AQ S Absolute Quantification Single 2 Plate Setup Target amp Sample Setting Dye FAM Sample List STD UNKN Update Tapet Last Target File Tempiste _ ed ip bye Name Te T ssayiD Foward Forward Tm Reverse Reveme Tm Producisize Prol FAM 7 Human FAM Mouse ID Type __ Name S34 oy Color nierRunCahbrs Onace Treatmer PrimingMethod Enzyme RnaOuarwy pnsCssnsy RnaQuaityMe DraQuastyt lt 1sTp 3 2 00E 004 MEM z ea 2 STO 490e lt 993 j ea 1 ra 3s i 8 00E 002 SN ad 4s a 1506 002 DEN iz zi s5 s0 2 00E 002 SE M4 6 STD 2 00E 001 at 7sm gt 200 000 SNE 4 8 STD iv 2 1 b e t ney oy Pel UNKN Semple1 z xa
3. mtDNA DNA mtDNA DE RMICEDEWC amp KHER al l 2 A DNA DNA PCR DNA DNA Ct to pg tao pg DNA DNA DNA
4. Thermal Cycler Dice Real Time System VII Appendix Hold Cycle 1 95 C 30 2 step PCR Cycle 40 95 5 60 307 GRH FAM 8 http www takara bio co jp 10 RR290 mtDNA FAM nDNA mtDNA Ct nDNA mtDNA 1 nDNA nDNA nDNA 6 02 x 102 X g bp bp 3 3 X 10 660 nDNA 20 000 pg 5 528 X 103 2 000 pg 5 528 X 102 200 pg 5 528 X 10 20 pg 5 528 x nDNA 2 NDNA NDN
5. 1 DNA VI A 2 DNA NucleoSpin Tissue 740952 10 50 250 NucleoSpin Tissue XS 740901 10 50 250 3 PCR e Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Single TP850 TP870 e Thermal Cycler Dice Real Time System Lite TP700 TP760 4 5 PCR 0 2 ml Hi 8 Tube NJ300 0 2 ml Hi 8 Dome Cap NJ301 96 well Hi Plate for Real Time NJ400 Sealing Film for Real Time NJ500 6 V V 1 mtDNA Symbol GO NM JE 5 Rplp1 9 NMO18853 Human_primer_Typel CHR3 ALAS1 NM_000688 Human_primer_Typell CHR7 PPIA NM_021130 Human_primer_Type III CHR15 NM_004048 1 M mitochondria
6. Thermal Cycler Dice SYBR Molecular Probe Inc Premix Ex Taq http www takara bio co jp 15 RR290 NOTICE TO PURCHASER LIMITED LICENSE L11 SYBR Green This product is provided under an intellectual property license from Life Technologies Corporation The transfer of this product is contingent on the buyer using the purchased product and components of the product solely in research conducted by the buyer whether the buyer is an academic or for profit entity The sale of this product is expressly conditioned on the buyer not using the product or its components for any Commercial Purposes Commercial Purposes means any activity by a party to generate revenue which may include but is not limited to use of the product or its components i in manufacturing ii to provide a service information or data in return for payment iii for therapeutic diagnostic or prophylactic purposes or iv for resale regardless of whether they are resold for use in research For infor
7. Ct 35 2 R2 0 97 gt 0 97 http www takara bio co jp 14 RR290 X gt gt DNA DNA mtDNA Ct Ct DNA DN
8. DNA 100 DNA 3 hES 1 X 109 DNA DNA a b pg Ct ia pg Ct 200 pg 21 62 20 000 pg 24 43 20 pg 25 21 4 000 pg 26 69 2 pg 28 88 800 pg 29 16 0 2 pg 32 79 160 pg 31 45 Y 3 718 X 30 103 Y 3 366 X 38 882 R2 0 9996 R2 iB 0 9997 c Ct DNA DNA DNA oa pg Gt pgy pg 1 19 51 24 32 21 168 21 875 3 230 5 22 32 ono 24 21 22 822 22 932 0 478 DNA A 3 23 B 0 478 C 0 059 http www takara bio co jp 8 RR290 B
9. http www takara bio co jp 4 RR290 V 2 A DNA mtDNA 4 DNA Mouse_Mt_primer 2 Human_primer CNV 3 CNV Typel 3 Type ll 7 BREA Type Ill 15 primer 1 B mtDNA DNA DNA VI A DNA DNA S DNA eee DNA gt J H DNA 100 pg ul 10 pg ul DNA 1 1 pg u 0 1 pg ul hES hiPS DNA
10. http www takara bio co jp 2 RR290 B DNA mtDNA PCR mtDNA DNA nDNA DNA nDNA mtDNA PCR nDNA Ct SDM mtDNA Gt Ct X nDNA mtDNA DNA 1 PCR E ll 100 25 IPCR 1 2 3 4 5 6 7 8 9 10 11 SYBR Premix Ex Taq Il Tli RNaseH Plus 2 X Conc 625 ul x 4 Mouse_Mt_primer 10 uM each 100 ul Mouse_genome_primer 10 uM each 100 ul Human_primer_Type CHR3 10 u M each 100 ul Human_primer_Type Il CHR7 10 uM each 100 u
11. Result Analysis Analysis Data Text Report Fluores cence CI Data Set of Each Well Data Set of Replicate A DNA OQty SDM DNA X 100 B mtDNA Qty SDM mtDNA nDNA mtDNA mtDNA mtDNA nDNA VII 1 primer mix gt Tm
12. 10 ng ul 2 ng u DNA Human Genomic 400 pg ul 80 pg ul DNA 2 1 DNA mtDNA mtDNA DNA x2 hES hiPS Human Ge nomic DNA DNA 1 1 x 106 1 x 107 DNA 3 3 NucleoSpin Tissue NucleoSpin Tissue XS DNA http www takara bio co jp 5 RR290 2 DNA DOIR a DNA 10 ng ul EASY Dilution
13. http www takara bio co jp 12 RR290 Plate Setup Plate Image Update LET A DNA Target List STD pg 100pg 2ul 200 pg B mtDNA Target List 1 STD 3 Hold Cycle 1 95 C 30 2 step PCR Cycle 40 95 5 60 C 30 FAM 4 Start Run Run File http www takara bio co jp 13 RR290 5 FAM Result Analysis Analysis Data Standard Curve Fluorescence 2nd Derivative Max R2 0 97
14. Type lll 15 Thermal Cycler Dice Real Time System 1 SYBR Premix Ex Taq Tli RNaseH Plus 2 X conc 12 5 ul Mouse_Mt_primer 10 uM each 1 0 ul dH20 95 ul Total 23 ul 1 SYBR Premix Ex Taq Tli RNaseH Plus 2 X conc 12 5 ul Human_primer 10 uM each 1 0 ul dH20 9 5 ul Total 23 ul http www takara bio co jp 6 RR290 Life Technologies PCR 1 SYBR Premix Ex Taq Tli RNaseH Plus 2 X conc 12 5 ul Mouse_Mt_primer 10 uM each 1 0 ul ROX Reference Dye or ROX Reference Dye II 3 0 5 ul dH20 9 0 ul Total 23 ul 1 SYBR Premix Ex Taq Tli RNaseH Plus 2 X conc 12 5 ul Human_primer 10 uM each 1 0 ul ROX Reference Dye or ROX Reference Dye II gt 0 5 ul dH20 9 0 ul Total 23 ul 3 StepOnePlus ROX Reference Dye Applied Biosystems 7500 Fast Real Time PCR System ROX Reference Dye Il 4 0 2 ml 1 23 ul 5 2 ul 6
15. b DNA EASY Dilution DNA 100 pg ul 10 pg u 1 pg ul 0 1 pg ul 10 DNA 10 ng ul 2 ng u 400 pg ul 80 pg ul 5 3 PCR a oi mtDNA GX DNA Human_primer_Type III primer Mouse_Mt_primer 1 2 DNA DNA DNA 4 4 DNA 10 ng ul 2 ul 1 FLET Primer mix 2 Human_primer ld CNV 3 CNV 3 primer 1 Type 3 Type ll 7
16. DNA mtDNA 1 DNA 1 NucleoSpin Tissue NucleoSpin Tissue XS DNA 2 EASY Dilution 4 DNA nDNA 10ng ul 1ng ul 100 pg ul 10 pg ul ica 10 DNA mtDNA 10 pg ul 3 PCR BEATA Rei O all Be nDNA mtDNA o WIS 2 2 FLET primer mix es Ce a primer Mouse_genome_primer Mouse_Mt_primer DNA ENT YT IV 10 ng ul 1 ng u DAAE jie 100 pg ul 10 pg ul 10 pg ul Thermal Cycler Dice Real Time System nDNA 1 SYBR Premix Ex Tag ll Tli RNaseH Plus 12 5 ul Mouse_genome_primer 10 uM 1 0 ul dH20
17. 9 5 ul Total 23 ul mtDNA 1 SYBR Premix Ex Tag Il Tli RNaseH Plus 12 5 ul Mouse_Mt_primer 10 uM 1 0 ul dH20 9 5 ul Total 23 ul http www takara bio co jp 9 RR290 Life Technologies PCR nDNA 1 SYBR Premix Ex Tag Il Tli RNaseH Plus 12 5 ul Mouse_genome_primer 10 uM 1 0 ul ROX Reference Dye or ROX Reference Dye II 3 0 5 ul dH20 9 0 ul Total 23 ul mtDNA 1 SYBR Premix Ex Tag Il Tli RNaseH Plus 12 5 ul Mouse_Mt_primer 10 uM 1 0 ul ROX Reference Dye or ROX Reference Dye II gt 0 5 ul dH20 9 0 ul Total 23 ul 3 StepOnePlus ROX Reference Dye Applied Biosystems 7500 Fast Real Time PCR System ROX Reference Dye Il 4 0 2 ml 1 23 ul 5 2 ul 6 7 PCR X PCR
18. A PCR DNA SYBR Premix Ex Tag II Tli RNaseH Plus RR820S A B NucleoSpin Tissue 740952 10 50 250 NucleoSpin Tissue XS 740901 10 50 250 Human Genomic DNA 636401 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Thermal Cycler Dice Real Time System Single TP850 TP870 0 2 ml Hi 8 Tube NJ300 0 2 ml Hi 8 Dome Cap NJ301 96 well Hi Plate for Real Time NJ400 Sealing Film for Real Time NJ500 Xl
19. A Ct 3 mtDNA Ct 2 mtDNA 4 mtDNA nDNA nDNA mtDNA mtDNA mtDNA nDNA X mtDNA nDNA mtDNA hES MEF 1 x 10 DNA NDNA DNA a NDNA pg nDNA Ct 20 000 pg 5 528 X 103 24 64 2 000 pg 5 528 X 102 27 81 200 pg 5 528 X 10 30 38 20 pg 5 528 33 93 Y 3 044 X 36 016 R2 0 9965 http www takara bio co jp 11 RR290 b mtDNA Ct mtDNA 25 40 3 074 5 528 hES MEF Ik 4 NDNA 556 DNA
20. Sima F RR290 IdRaRa Mouse Feeder Cell Quantification Kit v201312Da_2 1 hESC hiPSC er ey a etal Mouse Feeder Cell Quantification Kit pees hiPSC DNA A DNA PCR AR Sean DNA mt DNA 7 PCR mtDNA Ct
21. ation is conveyed expressly by implication or by estoppel This product is for research use only Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA M40 Thermostable RNase H This product is covered by the claims of U S Patent No 7 422 888 and its foreign counterpart patent claims M57 LA Technology This product is covered by the claims 6 16 outside the U S corresponding to the expired U S Patent No 5 436 149 M82 Tli RNaseH Plus This product is the subject of the pending JP patent application Iaf4R4 Tel 077 543 6116 Fax 077 543 1977 http www takara bio co jp DIV 7A WRIS v201312Da_2
22. l Human_primer_Type III CHR15 10 uM each 100 ul dH20 1 mlX 2 EASY Dilution for Real Time PCR 1 mlX 2 ROX Reference Dye 50 X Conc 50 ul ROX Reference Dye II 50 X Conc 50 ul Human Genomic DNA 100 ng ul 20 ul x Life Technologies PCR ROX Reference Dye StepOnePlus Real Time PCR System Life Technologies ROX Reference Dye Il Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 4 Thermal Cycler Dice Real Time System TP900 TP960 http www takara bio co jp 3 RR290 Il ETRE 20 C 80 C SYBR Premix Ex Tag ll Tli RNaseH Plus 4 6 80 20 4 6 20 V
23. mation on purchasing a license to this product for purposes other than research contact Life Technologies Corporation 5791 Van Allen Way Carlsbad CA 92008 USA or outlicensing lifetech com L46 SYBR Melting Curve Analysis The purchase of this product includes a limited non transferable license for all fields other than human or veterinary in vitro diagnostics under specific claims of U S Patent Nos 6 174 670 6 569 627 and 5 871 908 owned by the University of Utah Research Foundation or Evotec Biosystems GmbH and licensed to Idaho Technology Inc and Roche Diagnostics GmbH to use only the enclosed amount of product according to the specified protocols No right is conveyed expressly by implication or by estoppel to use any instrument or system under any claim of U S Patent Nos 6 174 670 6 569 627 and 5 871 908 other than for the amount of product contained herein L52 Rox Reference Dye Research Field Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 928 907 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research No right under any other patent claim and no right to perform commercial services of any kind including without limitation reporting the results of purchaser s activities for a fee or other commercial consider

Mouse Feeder Cell Quantification Kit

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