リステリア・モノサイトゲネス
Contents
1. 1 MF a EZ NR AA AAALW O ES 82. DNA DNA DNA 1 2 DNA 3 PCR DNA 10 DNA 1 PCR GeneLine PCR NNN
3. 10 eT NIR SI CNNIBASNIS 0 RI 2500 500 2 I 9 2
4. 3 3 16S 15 0 16S Rr 40mm Ph 16S 2 16S lie _ DNA PCR a 1
5. PCR Polymerase Chain Reaction 1 1 PCR 9 2 2 DNA 121 C 20 3
6. DNA PCR 0 2mL 2 200g 200mL 25g 25mL 3 25g 25mL 9 225mL 1 FDA USDA FSIS 4 DNA
7. c a b C d 3 FL 3 2B 4 16S g PCR DNA 16S 1B c 16S SS 2A 2B DNA 1
8. 3 DNA 16S 2 DNA 15 a ATCC 7644 ATCC 13932 ATCC 15313 ATCC 19111 ATCC 19112 Listeria monocytogenes ATCC 19114 ATCC 19115 ATCC 19118 ATCC BAA 751 NCTC 10890 Listeria welshimeri ATCC 35897 Listeria gray1 ATCC 25401 ATCC 700545 Listeria innocua ATCC 33090 NCTC 11288 Listeria ivanovii ATCC 19119 ATCC BAA 139
9. 2 SN 4 53 19 23 2003 DNA 1 1 1 DNA 5 5 8 2 169 1993 DNA DNA DNA 3 300 2646 3 3 A 029 847 7825 FAX 029 848 1256 URL http vww rdc nipponham co jp 2 8 a PCR b 2A DNA 1A
10. 0 2mL PCR PCR DNA 4 DNA DNA PCR 1 gt PCR 18uL DNA 2nL DNA 98C 10 94C 2 58 C 10 X30 94C 5 4 C 74C 20 2 PCR PCR PCR PCR DNA PCR PCR PCR
11. DNA 1 2 1 20 2 8 C A PCR 0 5mLX2 2 8 D 1 16S 2 6 IF a J 1 S
12. 2 GeneLine PCR 50uL 15 1 lt 2
13. 30mm 2 16S 1 Listeria monocytogenes ATCC19115 DNA DNA 50pg nL Listeria monocytogenes DNA50pg 1 5X10 CFU Listeria monocytogenes ATCC19115 DNA 25pg uL 1 2 DNA
14. 2012 10 4 1
15. uman a 0 a D 1 16S E 1 GeneLine A PCR 0 5mLX2 DNA D 1 B 4 12 GeneLine C 1 4mLX2 D 1 1 BE 1 1 PCR 1 454 460 2002 PCR DNA DNA 2 Paul S Mead et al Emerging Infectious Diseases Vol 5 No 5 607 625 1999 3 249 265 2004 DNA
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