TGP7000
Contents
1. 2 3 4 5 MR 6 2 10C 12 5 mLx 2 NASBA 10 110 uLx 1 a GP 7100 NASBA 10 60uLx 1 NASBA 202. 4 RNA RNA 1 1 2 NASBA NASBA Reaction Buffer dNTPs NTPs 3 IC IC 4 NASBA NASBA Enzyme AMV RT RNase H T7 RNA polymerase 5 NASBA 6 2 1 2 3 1 NASBA Nucleic Acid Seqguence Based Amplification RNA A NASBA
3. RNA IC IC NASBA ET IC DNA A 1 FT ee I FP 1 Kudoa septempunctata 2 RNA 1 3 TGP7000 1A510 1 1 PBS
4. 15 9 30 PBS 15 mL 1 2 1 2 1 RNase 2 20 30 C 2015 3 1 1 1 NASBA NASBA 55 pL 70 2 NASBA NASBA
5. r 4 IC r 5 6 r A 6 7 TGP7000 1A510 I 1 3 3 x 10 g 2
6. 4 15 a WW 1 3 2 3 15 2015 3 1 1 1 r 2 r IC 3 r r IC F EE A i 3 2 1 20 2 15 r 3
7. NASBA 30 10 70 3 20 30 C 2 1 10 200 1000 uL 2 1 5 mL 3 45 4 5 6 7 PBS 3 A 1 500 1 5 mL 100 pL 10 2 1 NASBA 1
8. B NASBA 1 NASBA NASBA 1 2 NASBA 5 u 3 A 2 2 5 10 2 RNA 4 45 C 1 5 45 C NASBA 2 5 5 45 C 30 3 NASBA 5 NASBA 6 C
9. GP 7200 2015 3 1 1 Compton J Nature 350 91 92 1991 2 36 19 24 2007 3 Sugita Konishi Y et al Jpn J Infect Dis 68 2 145 147 2015 113 0033 2 38 18 B03 3816 4480 FAX03 3816 6544 Ks 113 0033 2 38 18 03 3816 4485
10. Kudoa lateolabracis Kudoa thyrsites 1 1 CN 2 oo 3 2 1 2 2 3 NASBA 70 4 5 3 1
11. 4 NASBA 70 C TGP7000 1A510 C 1 20 30 C 5 2 NASBA 3 90 uL 3 6 7
12. B RNA 45 3 AMV RT H RNase H RNA T7 RNA polymerase 2 P1 P2 DNA 1 RNA ssRNA IC RNA RNA 5 T7 2 AMV RT P1 RNA DNA IE LIL LILITILLLLUELIIELI IIEI JFNess H AMV RT P1 C NASBA DNA
13. TGP7000 1A510 2015 3 1 Kudoa septempunctata 0 01 mm 2 3 7200 100 g 6 1 2 3
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