ポール・限外ろ過デバイス
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1. A ee lt E02 ND 2 a RE ND AN A RR EN gt EMI2. 5mL 20mL 0 05N Na0H 20 PES ug ng
3. a 6 i 9 i TFF OAD65C12 650 D 1 19 000 OA010C12 10K 1 19 000 OA100C12 100 K 1 19 000 OA001C12 1 K 1 19 000 OA030C12 30K 1 19 000 OA300C12 300 K 1 19 000 OA003C12 3K 1 19 000 OA050C12 50K 19 000 OA500C12 500 K 1 19 000 OA005C12 5 K 1 19 000 OA070C12 70K 19 000 OA990C12 1000 K 1 19 000 TFF TFF 2 TFF
4. 1 1096 5GmL 3 15 60 mL 9096 Av 7 0 n 7 s UN
5. GUS3 1 6L _ msp ly 2 2 A A ER SUS316L e 13 2cm x 22 6cm x 16 7cm 1 0 1 me 5 0 5 ms 20 mL 30 kPa 520 kPa 15 7 mm Zs shia
6. A PPT 2 6 3OK ComohC 15 UF 30 Comp PS 30 L TE Nanosep Device 7 lgG MW 156kD C MW 182 4kD 5 0mg mL O00uL 100K 1 000Xg 30 1 2 959 C 859 Do IgG 4
7. SK 50 g mL Cytochrome GC 10K 1mg mL BSA 30K mg mL IgG 100K 1mg mL Thyroglobulin 84 5 000g 1 10 0mL mL MCP003C41 3K 24 20 000 2S MCP003C46 3K 100 60 000 NM 3 3 5 95 99 MCP010C41 10K 24 20 000 MCP010C46 10K 100 60 000 a a SDS PAGE MCP030C41 30K 24 20 000 SDS PAGE
8. 30 7cm x 48 2cm x 20 8 cm 30 7cm x 48 2cm x 20 8cm 6 0kg 6 0kg 410kPa 410kPa 0 50C 0 50C 10 240 mL min 10 240 mL min lt 5 mL lt 5 mL 2 TFF TFF OAPMP110 115V AC 50 60 Hz 1 630 000 12 115V AC 50 60 Hz 1 264 000 OARES110 LV 4L TFF DN EA NSIM a 6
9. 20 15 10 mL 0 20 40 60 80 100 120 140 160 180 200 9 100 3kK 0 196 Protamin Sulfate in TxPBS 10K 0 025 Cytochrome C in TxPBS 30K 0 196 IgG in TXPBS 100K 0 19 6 Apoferritin in 1xPBS 5 000xg MAP003C36 3K 6 7 200 MAP003C37 3K 24 24 000 MAP003C38 3K 100 85 000 MAP010C36 10K 6 7 200 MAP010C37 10K 24 24 000 MAP010C38 10K 100 85 000 MAP030C36 30K 6 7 200 MAP030C37 30K 24 24 000 MAP030C38 30K
10. 9096 m ANSI SBS X 2004 EE A 1 8 cm 1 4 cm 12 8 cm 8 6 cm 0 25 cm lt 350uL 300L 34kPa
11. A A 316L V 304 TFF 0 EPDM i a 5 esan 8 3 cm NII RU 1 mL 121C SUS316L soo kPa
12. MWCO DNA 1 3 1 6 MWCO 3 6 MWCO 1 G MWCO0 1 6 MWCO 3 MWCO0 MWCO
13. 1 4 000xg 1 9 4 1 1 5mL 0mL 1 4 000xg 1
14. PCH Roche amp MF 5 15 50 500uL t GHP 9096 0 Eu gt 7 co i MF GHP ST 0 45um RE
15. 50 500 L OD003C33 3K 24 10 000 OD003C34 3K 100 34 000 OD003C35 3K 500 148 000 OD010C33 10K 24 10 000 OD010C34 10K 100 34 000 OD010C35 10K 500 148 000 OD030C33 30K 24 10 000 OD030C34 30K 100 34 000 OD030C35 30K 500 148 000 OD100C33 100K 24 10 000 OD100C34 100K 100 34 000 OD100C35 100K 500 148 000 OD300C33 300K 24 10 000 OD300C34 300K 100 34 000 OD300C35 300K 500 148 000 ODM02CS33 0 2 xm 24 10 000 ODM02C34 0 2 xm 100 34 000 ODM02C35 0 2 um 500 148 000 ODM45C33 0 45 um 24 10 000 ODM45C34 0 45 um 100 34 000 ODM4S5C35 0 45 um 500 148 000 MF GHP 50 500 L ODGHPC34 0 45 um 100 27 000 ODGHPC35 0 45 um 500 110 000 5 0 mL
16. Ge Ma CALD Pal corporat Macroser Aaa CevwvuoeW oa pH 3K 10K 30K 100K PES 0 2 um 0 45 um 7 2 cm 50 mm 12 cm 0 mL 820 7 2 mL 250 AM mL 80 LL 5 000 xg 14 000 xg 1 14 1 14 50 mL 70 450 uL 45 1 2 1 5 mL 34 1 5 mL
17. 6 MWCO MWCO INS MWCO A UA Od a oN CN SN 5 1K 3K 10K 0 34K 3K 10K 20K 0 5K 10K 30K 90K B12 1 3K 30K 90K 180K 1 4K 50K 5 nm 15 30 nm 150K 300K 5 72K 100K 10 nm 30 90 nm 300K 900K C 12 5K 300K 35 nm 90 200 nm 900K 1 800K A 13 5K 1000K 100 nm 300 600 nm gt 3 000K ag 14 2K ET 14 4K 17 8K MWCO o A 24 5K 1K 5 16 bp 9 32 bs B
18. 50 cm AWD 20emX88em 18em 30 40 mL min 5 50 pH 1 14 400 kPa 1 1 EN SA DNA HNA lt
19. 0 mL DNA 9096 20 mL 0 5 mL e e HPLC DNA RNA
20. David H Department of Biochemistry and Microbiology Rutgers university GHP 3K 10K 30K 50K 100K 1000K Sagm 0 45um 0 45 m 4 50 500uL 9 5 mL MF 2 0 mL 15 mL 15 60 mL MWCO Molecular Weight Cut Off MWCO 9096 KkD MWCO
21. a 0 28 cms 4 5 cm 500 L 15 LL 500 LL 5 UL 0 40 A 1 14 DNA RNA pH wp g 14 a ym a 14000xg 5 1 5 mL dn lt HPLC 7096 DNA 30K 100K se DNA 400 bp PCR 8 196 TAE 8 Life Technologies
22. Per 0 02 m L min 0 1 0 16 LV 4 0 4 11 16 3 m m LV T LV CM018LV LV 1 0 1 um 0 02 PSM10C12P2 PSM10C11P2 1K 0 02
23. K 250 8 200 8 150 3 100 50 0 2 4 6 8 10 14 20 24 30 0 1 mg mL 0 01 mg mL 4 4 YE a 100 OO 20 102 103 104 105 106 1 0 micron 60 0 Es A Es Es 40 30 ee g Cm 20 Reference Strumeyer Dr LC BSA 100K 100K 100K C BSA 100K 100K
24. 15 60 mL XR RN FD000K65 4 28 000 FD003K65 3K 4 30 000 FD010K65 10K 4 30 000 FD030K65 30K 4 30 000 FD100K65 100K 4 30 000 FD300K65 300K 4 30 000 OD003C65 3K 12 24 000 OD010C65 10K 12 24 000 OD030C65 30K 12 24 000 OD100C65 100K 12 24 000 OD300C65 300K 12 24 000 FD001X65 12 17 000 FD002X65 12 26 000 FD003X65 24 12 000 96 U
25. C600000000000000000000000000000000000000000000000000000000000000000000000000906066 _ 1 0 1 5mL oo 100 3 95 1 3 000 7 500xg 30 90 SS go 14 000xg 1 3 gs 4 80 1
26. 2096 R R R R 10096 N N 2096 R R R R 1009 N N 596 R R R R 6M R R R R 1096 R R R R R R R R 0 01M R R R R 0 059 R R R R 0 19 6 N Terg a zyme 196 R R R R 1M R R R R Ultrasil 11 296 R R R R 6M R R R R R N 1 15 http
27. SE NN _ HDPE 15 2 cm 6 6 cm 1 1 3 cm ciPPYRPEE 60 mL aE 60 mL a 0 40 C DNA RNA pH 1 14 3 000xg 250 mL NL 3 000 xg HPLC a RW ee Ke a 70
28. B 36K 3K 16 32 bp 32 65 bs 44K 10K 50 145 bp 95 285 bs 67K BE 30K 145 285 bp 285 570 bs 150K 50K 240 475 bp 475 950 bs l9G 160K 100K 475 1 450 bp 950 2 900 bs B 200K 300K 1 450 2 900 bp 2 900 5 700 bs 443K 1000K 4 800 5 700 bp gt 9 500 bs 90 650K SR 5 lgM 960K MWCO 50K 5 nm 15 30 nm 0 085 Am 100K 10 nm 30 90 nm 0 137 um 300K 35 nm 90 200 nm 0 212 um 1000K 100 nm 300 600 nm 1 4 a a a 5 19 01 0 2 IF3 7K GTY PO 9 100K 85 95 50K 96 99
29. 65 L PCR 6 5 mL 1 14 7 500 xg gt 14 000 xg pH 2 1 14 HPLC DNA HNA 1 7X100 mm 7096 Ei MN a 45 80 AL 34 100 pL
30. MCP030C46 30K 100 60 000 MCP100C41 100K 24 20 000 1 5 60ug 50 100uL MCP100C46 100K 100 60 000 5mL 3 SDS MCPM02C67 0 2 um 24 20 000 80C 10 MCPM02C68 0 2 um 100 60 000 4 MCPM45C67 0 45 um 24 20 000 56C MCPM45C68 0 45 pm 100 60 000 5 Laemmi U K Nature 227 680 685 1970
31. ss 14 uL PC TR 96 350uL Tr 1 Em 4 8033 3K 10 8034 10K 10 8035 30K 10 8036 100K 10 100ug mL 75 L MLM1 M2 M3 M4 M1M2 M3 M4 M1 M2 M3 M4 ML 67 8kPa 8 80 kD 30K 80 kD ww ww ww GA we wo wm 5 L SDS PAGE 50 kD 40 kD 30 kD ML MI BSA M2 BSA Cyt C M3 BSA Carb Cyt C M4 20 kD le TFF 1L
32. 100 85 000 MAP100C36 100K 6 7 200 MAP100C37 100K 24 24 000 MAP100C38 100K 100 85 000 MAPM02C67 0 2 um 24 24 000 MAPM02C68 0 2 um 100 85 000 MAPM45C67 0 45 um 24 24 000 MAPM45C68 0 45 um 100 85 000 1 5 50mL 50mL 3 1 000 5 000xg 30 90
33. 2VN IOOK BSA 60 B65 VR300K BSA gt 97 lgG gt 8596 S 55 500 kPa Per 0 1 m L min 0 5 0 8 FS001K10 1 0 1 um PSM10C12 PSM10C11 CM00 S 3G2V 1 0 2 um PSM20C12 PSM20C11 QL25N 1 0 45 um PSM45C12 PSM45C11 322DB 1 0 65 um PSM65C12 PSM65C11 T TFF 1 K OS001T12 0 3N 30 K OS030T12 50 K OS050T12 70 K OS070T1
34. www pall com Japan laboratory asp Ccc 163 1325 6 5 1 103 0022 2 2 1 03 6214 1090 TEL 03 6386 0993 FAX 03 6386 0994 541 0048 2 5 1 06 6231 1672 812 0007 2 22 3 092 414 9361 lt lt http www kanto co jD E mail reag info gms kanto co jp 201 1 5 10 08 1 1 50V
35. Microsep Adyances et aa eos PALD Pall Corporation DNA 9096 3 3cm Go gt OS 3K 10K 30K 100K PES Oem OA A 3 3 cm MM HPLO SPP _ FE T a em a i ss 34 45 80 L
36. 2 100 K OS100T12 300 K OS300T12 14 109 R R R R 1N R R R R 1N R R R R 1N R R R R 1N R R R R 709 R R R R 1096 R R R R 7096 R R R R 7096 R R R R 7096 R R R R 1N R R R R 1N R R R R 1096 N N 1009 N N N N R R R R 0 196 R R R R ee 0 89 R R R R 0 USUIo 10096 N 1096 2096 R R R R 1096 R R R R 2096 R R R R R R R R
37. LPLTO12c Life Sciences 9 1 Filtration Separation Solution sm m m DNA m m BSA 500 1 0 mg mL 450 PES 30K 400 3 30
38. OS001CT02 LV0010 1 0 2 um 0 02 PSM20C12P2 PSM20C11P2 5 K 0 02 OS005CT02 QL25N 1 0 45 um 0 02 PSM45C12P2 PSM45C11P2 10 K 0 02 OS010CT02 322DB 1 0 65 um 0 02 PSM65C12P2 PSM65C11P2 30 K 0 02 OS030CT02 50 K 0 02 OS050CT02 70 K 0 02 OS070CT02 100 K 0 02 OS100CT02 T TFF 0 3N 300 K 0 02 OS300CT02 13 1 1 55L 0 1mg 0 5mE
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