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1. 1 T 305 8575 1 1 1 12. 2 2 1 2 DNA 47 197 65
3. 2 1 270mm 34 mm 100mm 620mm 50 3 6
4. 1 10 KRX6
5. 3 C 31
6. 2 130m 1979 2
7. 1 4 TEM 5 Olum FIB 2 TEM RAPP 3 AEA 4 SMI2050 Rev 3 1 22 00000000000000000000 OHHHHHHHHH 0000 HHHHHHHHHHHHH HHH HHH 00000 305 8572HHHHHHHHHH 1 1 1 um me Oo oO x 5 1 mm
8. 4 SHR SHR 5 1 D Sutoo et al Brain Res Bull 22 1989 565 569 2 D Sutoo et al Brain Res 933 2002 1 11 3
9. 2 14 10 7 15 1 2 4 3 0 48 mm m 0 09 Imm m 76 7 EU b T 2 21 FI
10. 1 CO Brain Mapping Analyzer
11. KEK 1 PDF Portable Document Format Adobe Systems Adobe Acrobat Illustrator PDF s PDF file Windows Mac UNIX PDF file OpenOffice 1 1
12. WW 4 DLB OT 2 2 1 5 9 D Sutoo et al J Neurosci Methods 85 1998 161 173 2 D Sutoo et al Brain Res Bull 22 1989 565 569 3 D Sutoo et al Brain Res 933 2002 1 11 4 D Sutoo et al Brain Res 418 1987 205 213
13. 1 UU 1 2 1 Y f 1 E mail chigira sakura cc tsukuba ac jp 3
14. mRNA LMD ab 2 E 5 I SW sss SLE RA SLE
15. E MS MS MS MAPMAKER SIBS ver2 3 4 4q35 5 5q31 33 6 6p22 21 3 12 12q14 24 2 13 13q14 14 3 TNFa
16. 4 2 5 1994 1 11 2 ID 1983 3 1997 4 1979 30 KER FA 28 B g il E 425
17. O DOLD 0 0000000 Haa oo oo 8 SS 2 z gt gab a aad 1 goaa ms ria BARR HEH iriri HORE ooo
18. 4 1 3 1 2 7 20 330mm 33 209 000mm 1 E mail tsuchiya kurofune shimoda tsukuba ac jp http www shimoda tsukuba ac D 535mm A 535mm 330mm 2 660mm 535mm 450mm T amp 23 450mm 1 50mm 500cm 2 120cm 11 3 8 05 2 1 4 120cm 2 v Y 2
19. 6205ZZ V AC 6205SLLU V 6205LLU 6205
20. TEE B EEE oo mr i jar Tj mn on LJ ao ad A o AO A O LIL A ca CC 0202 5 5 O go GO or HHHHH mm mm lt SR HHHHHL ga EE
21. 18 LDM nested RT PCR 19 Web 20 GAMMA10 21 jj 99 seesaararaar nrnna 23 24 A BIRE RRR 25
22. 3 1 3 3 1993 7 16 17 1993 7 20 21 1993 8 7 8 3 1985 8 24 27 1981 8 4 8 H 1987 7 28 29 3 1983 7 27 2 6 1984 8 4 6 1985 7 H 28 30
23. IgA IgA KR E ATER ERSTE IgA MIRA IgA Cb Hemophilus parainfluenza DESK DUZ x REK Staphlococcus aureus IgA ELISA Staphylococcus aureus 2 N 290 N 16 8325 IgG proteinA Western blot Staphlococcus aureus proteinA
24. 1980 7 7 3 GAMMA10 1 5 5 56KA 9 10KA 0 3 12 Plug Barrier Anchor Central 2 48m 7 6 0m SDE GAMMA10 41 1 1 1
25. z EE 1070 gt 3 5 Haz e A 23 Y cm 2 A 1 mi lu manu mu 16 5 PREY 305 8573 1 1 1 l E
26. 1 Y Yokouchi S Ito et al Significant evidence for linkage of mite sensitive childhood asthma to chromosome 5431 433 near the interleukin 12B locus by genome wide search in Japanese families Genomics 66 2000 152 160 2 S E Noguchi et al Evidence for association between plasma platelet activating factor acetylhydrolase deficiency and increased risk of childhood atoplc asthma J Hum Genet 47 2002 99 101 18 LMD nested RT PCR 305 8575 1 1 1 1 PCR PCR PCR
27. mn ria ri me EE ES pas SEE Fara EE 6 a maa a T E HEE BERHE ERE iaia s ea A ea oo o HL ET 5 0 1 do d Ad 10 120 10 10 1
28. 4 RITAS 4 MNO 4 Tel 029 853 4879 1983 1997
29. VRVS http www vrvs org Viewstation 3 14 12 Asia Pacific Miniworkshop on Lattice QCD 15 1 23 24 International Lattice DataGrid ILDG Workshop http www lqcd org ildg 14 12 19 15 5 2 CE Hk 15 12 5 4 ILDG Workshop Unix Windows
30. ZIME L 24 2 6 2 DORREA MTF ALT D 3
31. A AA LILTLTLTL TLT LILTLT 00000 1110 10 10020 1 TLT LILTLTLTL TLT A A A A A A A A A A A A A A A A A A A LILTLTLTL TLT LILTLTLTL TLT n_n pass EI sesi sana l usa Bm P iss n_n E L L_ O NE i ow Ei enton qa A E LIZ LID LIL 1 L L O A LIO 202903 LI L L L O G a DOL O Es Hano E j oa o ENE E BE EE L HEY HEE Ee 22 iss a
32. n A A A A A LILTLTLTLT LILTLTLTLT LILTLTLTLT O A A A A A A A A A A A A O A A LILTLTLTLT LTLTLTLTLT LTLTLTLTLT A A A O A A A AA O A A A A O A A A O A A 10 120 O A A A A A A A A dO A A A O A LILTLTLTLT 341 A G 7 02 E ES 02 sn r O A ao LILI oo o iss 00 g ETI LIL C AK SSE een oss gaa GORG O00 ono 00000 pse pan ES T Rg es mas ama 5 F TEST ESE TES P me PS L a men
33. 1 20 Brain Mapping Analyzer TIRAL RRS 3 2
34. IgA RIERA 60 70 HOEA 22 Hv ELISA 00 1 A Koyama et al Glomerulonephritis associated with infection A possible role of bacterial superantigen Kidney Int 47 207 216 1995 2 IgA 30 Vol 46 No 1 1999 28 000 HLA UU DYNAL RELI SSO 00000000 00000 00000000 0 0000 HLAHH HLAHHHHL HHHHI HHHH HHHHHHHHHHHHHHHHL 0305 85750 000000000 1 1 1 oun mm O HLA r ETIS 0 lt THEE 22000 HH 0 E say O_o agaaga gogogaga OGG EEE IEE L L a L L sss sma usa as A g HLA DNA 000 00000 H12 IIHHHHHHHHHHL 7 wwW OtnW Or jD 0000000000000 HLA 000 00 SSP HLA DNA 00
35. QGENESCAN GENOTYPER E mail s itoh md tsukuba ac jp Tel 029 853 3352 2 3
36. wwwrbiol tsukuba_acjjp 5 oO gt 1 LILTLTLTLTLT LILTLT LTHLTLTLTLTLT LELHLTLT LILTLTLTLTLT L L o o d L P L A ng ag 1 gaad g a g a ad A g a LIL a oa a A oa a dcad adau ad A g A g a do a d aa aa A g aa ad Ad gG LJL Io 1 662590 rL LP rL
37. 305 8577 1 1 1 1 DOZAT halls 2 1 RealNetworks RealSystem
38. O 1 1 es o_o IE TES ES 0 o o ma si 00000000000 20030000060 Tel 029 853 4879 Tel 029 853 6669 http www biol tsukuba ac jp tjb 23 ub 27 RIBE K O HERA E lt DIE T 305 8575 1 1 1
39. Unix Windows EI TN 6 ee 1 2 Unix Windows Perl TE FORTRAN C Windows Perl 3 JAVA Java GUI AWT Swing 2
40. D E mail akiyamak md tsukuba ac jp Tel 029 853 3330 gt E mail dsutoo md tsukuba ac jp Tel 029 853 3113
41. UU 5 IS0100 1501600 4 L UU 1 Progress of Digestive Endoscopy vol 20 110 118 1982 2 20 4 191 195 1982 3
42. Unix socket Windows winsock C G Unix Windows Visual CH GUI Unix Glade 100 200 300 400 500 JP512 1 1 b Reading home hiromi DAQ DATA EdE 24 done Enter the new BAN X 285 212 Z 0 Java for 2 x ADC Gain PHA MCS File Refresh Sync 512 1024 2048 4096 data clear 0 1023 09 47 23 7 Cursor 548 Count 13 lt lt gt gt Log ROI 582 gt 714 1606 42 GTK 1 http www tac tsukuba ac jp hiromi 2 http www gtk org 3 http www g1mp org tml gimp win32 4 http glade gnome org lt il D PDF web lt 305 8577 UTTAC Annual Report 2 PDF web PSJPDF
43. 0O 1X10 ml sec 9 0 AC620SLLU 6205ZZ 2 0000000000000000000000000000 HB 0305 8795 000000000 15151 oo oo oo oo oo on
44. 23 3 5 110 128 1985 4 35 1 210 215 1986 5 UV 105m OT 23 2 2 44 48 1985 14 MR 305 8573 1 1 1 MR RF RF
45. AWT 1 Jaa FORTRAN GUI Windows Java IO Java applet 2 6 Java Unix Windows MacOS CPU 4 GTK GIMP GUI Unix Windows T 305 8577 1 1 1 GTK
46. NO b 3 LMD mRNA Lio BERI ORTER ARRE A D DRERI T D RNA RT PCR c DNA 4 SLE DRRR 1 RA 3 LMD E mail eonose md tsukuba ac jp Tel 029 853 3332 OCT C 80C LE 5 LMD LMD 80C LMD
47. ALS 6 8 10 A Sandhoff 2 3 S 2 7U7 1 8 HEX GM2 GM2 GM2 HEX A 2 HEXA a HEX HEXB 8 HEX o Tay Sachs HEX B
48. MR MR 4000 MR 4 8 MR 4000 MR _ 15 305 8572 1 1 1
49. TERZA 415 0025 5 10 1 SCUBA 1 0 10m OH 10m
50. ze 2 S m ee EEE E N uma OTHE HE O Es E n a n a am L Y Esa E Ha oo gooo Ei or HEHE TE O goood OH
51. Fas a A 6 5 25 LAA Os 2 12 Adobe Acrobat PDF file PDF 3 http www swtoo com product enfocus product pitstop50 pitstop_50 html 12 O 2 305 8575 1 1 1
52. EL 40 20 DIC 1270 HE F EL EL E mail dsutoo
53. A Sandhoff 15 23 ALS 35 2 2 1 Tay Sachs 4MUG 4MUGS 4MUG TH HEX HEXB 4MUGS HEXA 2 2 HEXB DNA HEXB exon exon intron junction PCR ABDI mRNA
54. PNH PIG A Z u FCM two color UKE CD55 CD59 MDS PNH FCM PNH MDS FCM PNH 1 PNH CD55 CD59
55. coating buffer BSA Tween PBS Blocking 100 37 1 Human IgG IgA IgA1 IgA2 peroxidase 37C 1 ABTS 15 2mM 405nm 3 DELISA Wstemblot Staphlococcus aureus protein 2 ELISA Staphylococcus aureus IgG IgA IgA1 IgA2 IgA MRSA RA LL IgA CI Staphylococcus aureus IgA Staphylococcus aureus IgAl IgA 35KDa DIR DIRTY Cl3 Staphylococcus aureus cell envelope antigen 3SKDa
56. 3 2 UU rn on on 1012 129 A A LILTLT E mail gojingmaru craftsman gseijutsu tsukuba ac jD 26 000000000000000000000000 0000000000 0000 000 00000000000000000 305 8575 0000000000 141 1 su N amu Esa ass 1 en tz ma O a aa ET LIO a a L A d G aAa aAa aAa O O a aa aAa ada Ad A O O ag aa aAa ada d aG aa aAa ada aA LIP d aG a aAa aAa aA A O d aG G aAa aAa A A AA d 0 O aA A A A AA 1 a A A A A 00000 Fa l EER ma a EE
57. Sama mm a A 0000000 HHHUHHHH 000000 000000 HEY Ei HRG Es pam HEHEHE OOO o_o ES am sss raq iom 8 TT em he Ei FE j FET Ws FS
58. ESE msn EE a 000000 fm r mo EE g EERE HERE a EE yi hm a ram gani pas a e e HEE EEE IE man Be usa mmm Emi E pa PE pea T
59. s MA MS MS Mac PDF file e NN I i LIV E web o 4 PDF PostScript PDF file PostScript file Script pitStop 48 PostScript file s Distiller PDF file PDF file 5 PDF PDF file
60. FS O o HEH Gira IES ES EE IE m 1 EET EE ETE mr asal ooo anara HET EE Ei EE EAE Hcr EET MEET HEHEHE EE IEE ushi sai oo 00000000 Human Brain Mapping 11 2000 93 103 J Neurosci Res 63 2001 369 376 J Neurosci Methods 85 1998 161 173 Neuroscience 58 1994 227 234 Neuroscl Res 35 1999 339 346 XIIth International Congress Pharmacology M nchen Germany 1998 2 E mail akiyamak md tsukuba ac jp Tel 029 853 3330 E mail yabek md tsukuba ac jp Tel 029 853 3231 3 E mail dsutoo md tsukuba ac jp Tel 029 853 3113 sma siluma Pisi T 305 8575 1 1 1
61. RT PCR exon2 5 pCR Script Amp SK vector jo s 3 HEXA HEXB 10 1 78 1 3 heterozygous carrier Sandhoff HEXB 2 Intron2 exon3 junction G A TVS2 1G gt A exon13 RS33H 533 AEAT OS EA 4 ALS
62. encoder PC Helix Producer9 0 RealMedia WW encode Helix Universal Server Broadcast PC Windows XP Dual Xeon 2 2GHz Mem 1024MB DualHead Video ViewCast Osprey 210 Helix Producer 9 0 VRVS 3 0 gt Canon VC C4R 806 WRU 806x3 Sony WRT 804 gt XLR RCA 3m Sony RS 232C 30m RCA BNC 20m Polycom ViewsSationFX BRI Epson ELP 715 lt VRVS client u ee VGA 20m 1 Polycom O Viewstation Viewstation
63. UU L OUO OL 20041 161 ars LE EEF 000000000 6 0 0 0 00 060 0 6001 SBS B 1 hali E A 98 Bouea E EEE RET a 00000 261 40 00000 0 0 0 0 600 00 06000 0 0 00 HEM ESE ES S E EE E 00000 0 0 00000000000000000000000000 EE sam 00000 EEE gu EEE 000 HHHHHHL 9 41 0 090 0
64. http www tsukuba ac p map access highwaybus html JR 202200 000000 26250 D 22 2570 8 E IE a 2600 gt gt MN 6 http Wwwwr hosp tsukuba ac jp annalmap htm w UAE EFS i E a ure HHHHHH Hunin n momo HHHHHHHH AE 000 3 9 40 9 55 AH f 10 00 12 00 10 00 10 20 10 20 10 40 10 40 11 00 11 00 11
65. 029 853 3034 000000000000000000000 000000000000000000000 http www tac tsukuba ac jp tech2003 iB i L tech tac tsukuba ac jp 029 853 3326 0O OL 000 000 00000000 00000000 0 0O OL 000 000000000 11 1 000 000 000 000 000 0O OL 000 000 000 0O OL 000 1 0O OL 000 000 NN 5 A A A A A ME A 1 10 12011020 TERES TL TI LIL 1 E 0 U SS S sm j SEE 1 1 120 LTL TL TI p JE INE TE TED EES d A A A AA AA A FJ L LI C 25 C E PDH 160 30 16100 0 00000000000000000000000 http www tac tsukuba ac Jp tech2003 15 t J o H 6 E lt 0000 0000000 000000000600
66. pa IPS sssi see Eq EE F 5 no Dn TE 27 ELISA Staphylococcus aur
67. Gp CD16 b CD14 CD24 2 EDTA n 5 MDS n 4 PNH n 2 PBS 30 buffy coat 4 30 FACSort Becton Dickinson CELL Quest ESC SSC CD13 B CD19 T CD3 02 CDSS FTTC JS11KSC2 3 IsG1 Immunotech 59 282 H19 IgG2a BD Biosciences 14 lgG2a Dako Cytomation CD16b FITC 1D3 IgM Immunotech CD24 MLS Phar Mingen CD3 PE PC3 188A lgG1 Dako Cytomation CD13 PE WM 47 IgG1 Cytomatio
68. Sanqhoff 7 _ 24 MAJIK 305 8577 1 1 1
69. 2 2 single nucleotide polymorphism SNP MS MS MS 1 4 MS 10cM MS F 100 400 bp PCR 57 FAM f MS Su Qon tO p 2 Z 386 MS l s PCR DNA DNA ABI model 377 MS
70. MER 26 CE 27 ERE ELISA staphylococcus aureus 28 __ Sa W us 29 Carex 30 sessearren rrano 31 EERE 00 80 Li 0000000000000 ul HHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHUHHHHHHLH 000000000000000000 00000000000000000 00000
71. PC TAPIRA LED ER LMD 6 nested RT PCR T nested RT PCR RNA ISOGEN M MLV Oligo dT cDNA cDNA 2 KH cDNA T TCR B nested RT PCR 7 nested RT PCR T c DNA D T 47 99 cDNA 59 2 RNA
72. D30 FH 100mm 390nm 520 nm 360nm 1 2 360nm ERLU ZAZE V 390nm 520nm 3 20nm
73. 1 2 RT PCR cDNA 1 Luzzi V et al Am J Pathol 158 2001 2005 2010 21 Murata et al Arthritis Rheum 46 2002 2141 2147 3 Kolble K J Mol Med 78 2000 B24 25 19 HHHHHHHHHHL 000 000 0000000000 0000 000000000000000 J 305 8577 0 000000000 1 1 1 o a a A A o d aA a A A a so Em BE EE zz mm
74. 5 D Sutoo et al Physiol Behav 52 1992 865 872 6 D Sutoo K Akiyama Physiol Behav 60 1996 177 181 7 D Sutoo K Akiyama Brain Res Rev 25 1997 1 26 8 Akiyama D Sutoo Brain Res 823 1999 154 160 9 D Sutoo K Akiyama Neurobiol Dis 13 2003 1 14 13 BENER T 305 8576 2 1 1 1 X G 2
75. D Sutoo et al Neurosci Lett 82 1987 297 302 4 D Sutoo K Akiyama Brain Res Rev 25 1997 1 26 5 D Sutoo K Akiyama Neurosci Lett 269 1999 133 136 6 D Sutoo et al Eur J Pharmacol 155 1988 189 192 7 D Sutoo et al Brain Res Bull 30 1993 107 113 8 K Akiyama D Sutoo Brain Res 823 1999 154 160 9 D Sutoo et al Eur J Pharmacol 183 1990 1313 1314 OE 0 305 8575 0000 00 0000 1 1 1 LILTLTLTLT A A A LILTLTLTLT A 0000 an ee i GE VE HEHEHE IE HE HEHE 00000000 TESTES 5 9 es wo a gg oo re ee po s Sm
76. 20 11 20 11 40 11 40 12 00 7 8 O sU i taa 9 L oz 10 Unix Windows saa aes 11 pdf web 19 12 00 13 00 13 00 17 00 13 00 14 00 14 00 15 10 15 10 15 30 15
77. 30 15 50 15 50 16 00 r 6 18 30 CN a 13 14 S K 4 16 00 16 20 16 20 16 40 16 40 17 00 NEHER B MR 15 16 17 OELE 14 00 15 10 P 17 1 7
78. Adobe Acrobat PDF fie MS Word 2 PDF Writer Acrobat Distiller PDEF Writer Distiller PDF Writer Acrobat 5 0 MS Office Acrobat PDF Maker PDF file Acrobat 5 0 PDF Maker Distiller FOCWA web l http www tac tsukuba ac jp uttac thesis html 2 http www tac tsukuba ac jp uttac annual 3 http www tac tsukuba ac jp tech2002 abstract 3
79. md tsukuba ac jp Tel 029 853 3113 gt E mail akiyamak md tsukuba ac jp Tel 029 853 3330 D PL YE 2 E LFF TE DF SDR 9 H SHR
80. 0 10 10 10 1012 1 20030 100 20030 110 HK a kil 0 00000000 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 1200 1800 1600 1400 1000 20 0 v 000000000000 RID 2 127 49 400 1 3 http www tsukuba ac jp robots ja index html IP 2003 11 12 http www tsukuba ac jp 2 http www namazu org GAMMA10 IEIET T 305 8577 GAMMA10 5 3 2 A GR a
81. 0000 square umin ac Jp JSHI frame html HPB HHHHHHHHHL 20 O a L oo o ag a H ogo g H o g oa H LIDIA IEE 0 00 HHHHPCRHHH HAD DUD 0 000000000000 L 7 O lt AO O x aa a oa aa G O O Micro SSP Japanese HLA NA Typing Tray ONE LAMBDA INC 21HHHHHHHHUHHUHHHHHHLH 22 DNA 10 J SSP ll SSO Carex 305 8572 1 1 1 1993 7 6 8 1 7 Carex JII 1994
82. 0000000000000 000000000000000000 0 000000 0 00000000000000000 000000000000000000 0 00000000000000000 000000000000000000 000000000000000000 000000000000000000 0 00000000000000000 HHHHHHHHHHHHHHHHHHHHH 00000000 HHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHH HHHHH HHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHH HHHHHUHHHHI HHHHHHHHHHHHHHHHHHHHH HHHHHHUHHHHHH HHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHH 00000 HHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHH HHHHHHHHH U U HHHHHHHHHHHHHHUHHHHHHLH 00000000000 000000000000000000 0000000000000000 0 00000000000000000 000000000000 00000000000 00717 305 8575 1 1 1
83. B 305 8573 1 1 1 FIB SMI2050 14 21 COE 7 15 1 SM12050 Focused Beam Q O 5
84. CD55 CD59 B 4 CD16 b 96 5 3 8 HEK CD24 99 8 0 1 14 85 2 9 5 B CD24 93 4 0 8 MDS PNH 4 FCS T CD59 CD55 B PNH CD16 b CD14
85. eus T 305 8575 1 1 1 1 IgA ELISA IgA IgA IgA1 30 40 20 30 49100003 35 IgA IgA1 IgA1 FSET lC KD IgAl methicillin resistant Staphlococcus
86. n CD19 PE HD37 lgG1 Dako Cytomation 3 1 D CD55 CD59 ZME LF CD59 99 9 0 1 CD55 86 9 8 3 CD59 CD55 99 8 0 2 CDS9 99 9 0205 2 NE CD55 99 0 0 8 CD59 69 7 16 1 G 0 59 MsIgG2a CDS9 fa B CD55 99 5 0 4 CD59 95 5 3 4 T CD59 95 9 2 3 CD55 52 9 12 2 2 MDS CD55 CD59 3 PNH
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