コシヒカリ鑑定団 DNA解析技術の理解に最適な新しい教育用キット
Contents
1. 4 14 H Visi vaio T 839 0864 1 1 Tel 094236 3100 Fax 0942 36 3101 URL http www visionbio cojp E mail infog visionbio com2. PCR 0TG60AAMTTATTWTTATTATYTMTTAMTG Forward Primer Reverse Primer CT6CCAA ACGetrr 6 6 AATAATAATAATAATAATAGGCATAG T 13 1 2 DNA 15 25 C 6 20 PCR 1 2 3
3. ver 2 Cat No 01 2020 Visi io 0 f 1 No 1 400 2 BL 3 24 10 4 DNA PCR DNA 5 2 6 DNA 7 PCR DNA 8 PCR
4. 30 30 DNA 1 5mL 30 02mL 1 PCR 30 PCR 0 2mL 2 20nL UV 1 50g 30 1 2 DNA 10
5. gt 3 1 3 5mm 3 5 PCR 2 1 DNA 2 PCR 1 PCR 2 1 5 0nL 1 PCR 5 0uL 2 PCR Cycle 35cycles Hold 94 C 55 68 4 5 1
6. 25 7 1 20 96 10 JU 2 20 20
7. 9 J 24 20 9 6 1 37 5 2 9 8 O 3 9 5 O 4 7 3 QO 5 3 1 O 6 3 0 O 7 2 7 QO 8 1 8 O 9 397 1 8 O 10 1 4 O 11 1 3 O 12 1 3 X 13 1 1 x 14 0 9 O 15 0 8 O 16 0 8 O 17 0 6 O 18 0 6 O 19 0 6 8 20 0 5 x
8. 11 1 35 DNA 12 microsatellite 100 SNP Single Nucleotide Polymorphism DNA RC
9. 5 MP 1 2 3 4 M 100bp DNA ladder P 1 10096 2 3 10096 4 1 DNA 104L 2 3 2 5 Agarose Gel 0 5 x TBE buffer 100V 40 50 1 DNA 2 PCR DNA PCR 3 1
10. 50 bp 180 bp 1 M 100bp DNA ladder P positive control DNA 1 10096 2 3 10096 4 4 596 M 100bp DNA ladder P positive control DNA 1 20 2 10 3 5 4 2 5 1 6 0 10 PCR Polymerase Chain Reaction DNA 20 DNA DNA 3 DNA 2 1 DNA srEP2 55C STEP2 55 C 2751 DNA PNA
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