Hybex Microsample Incubator User Manual
Contents
1. 2011 21 0 Use at temperatures up to 99 C 2011 13 0 96 Well PCR Plate non skirted 50 cs Seal with 8 Strip Flat Caps 5x10 CS cat 2011 22 0 using PCR Plate Roller cat 2011 21 0 Use at temperatures up to 99 C 2011 20 0 PCR Plate Sealing Foil 100 pk Use with 96 Well PCR Plate skirted 100 PK cat 2011 10 0 or 2011 11 0 at temperatures below 80 C 2011 21 0 PCR Plate Roller For applying PCR Plate Sealing Foil cat 2011 20 0 2011 22 0 8 Strip Flat Caps 125 pk Use with 8 Strip 0 2ml PCR tubes cat 2011 125 PK 30 0 and 96 Well PCR plate non skirted cat 2011 12 0 or 2011 13 0 2011 30 0 8 Strip 0 2ml PCR tubes Seal with 8 Strip Flat Caps cat 2011 22 0 125 CS 2011 31 0 0 2 ml PCR Tube with attached flat cap 2x500 cs 2x500 CS 2011 40 0 0 6 ml Microcentrifuge Tube with locking cap 2x500 cs 2x500 CS 2011 41 0 1 5 ml Microcentrifuge Tube with locking cap 500 cs 500 CS Version 1 1F November 2012 11 408 733 7337 techserv scigene com Sc G CO nN CO Hybex Microsample Incubator USER MANUAL Vill APPENDIX A Using and Calibrating a Watlow SD31 Controller For selected Hybex incubators manufactured between 2005 and 2010 A Using the Watlow SD31 Temperature Controller The controller has a single LED panel with four buttons The set temperature is displayed if the SET key is pressed and held otherwise the ACTUAL block temperature is displayed To set the block temper2. Microsample Incubator USER MANUAL Follow these steps to adjust the controller to achieve accurate temperatures 1 Insert a block into the Hybex incubator turn ON the BACK OF UNIT instrument and set the controller to 65 C Allow 15 minutes for temperature to stabilize 2 Using the cable provided with the digital thermometer plug one end into the blue receptacle found on the back panel of the Hybex incubator and the other end into the digital thermometer 3 Turn on the thermometer Allow 1 minute for thermometer to stabilize The actual temperature of the block will be displayed 4 Calculate the difference between the digital thermometer and the temperature controller to Digital Power Cord Thermometer Receptacle determine the adjustment value For example if the Jack j thermometer reads 63 9 C while the controller reads 65 C then the adjustment value is 1 1 C 5 On the controller press the up and down arrows simultaneously for 3 seconds The left display shows A1 and the right display shows open 6 Press the Advance Button green circle 3 times until the right display shows i CA The left display will show the offset value between the controller and thermometer when the unit was last calibrated 7 Using the up or down arrows add the adjustment value from step 4 above to the existing offset value shown on the controller For example if the adjustment value is 1 1 C and the current
3. Temperature Controller teste rel right display green The Hybex Microsample Incubator is equipped with a Watlow EZ Zone temperature controller The controller has two LED displays and four push buttons The left display shows ACTUAL block temperature in red The right display shows the SET or programmed temperature in green To enter the SET temperature simply push the up and down arrows until the desired temperature is shown in the green display on the right The unit will adjust the heat of the block until the SET temperature is attained Advance Button Up Down Arrows Infinity Return Key The controller is calibrated at the factory to provide accurate block temperatures from 35 to 99 C SciGene recommends checking calibration monthly See section D Calibrating the Temperature Controller for details E Calibrating the Temperature Controller The temperature controller comes calibrated from the factory to provide accurate block temperatures from 35 to 99 C The temperature controller will require calibration only if e When checking the block temperature with a calibrated digital thermometer the block temperature differs by more than one degree 1 C from the actual temperature shown on the controller display An NIST calibrated digital thermometer sold separately SciGene Cat 1051 52 0 is required to calibrate the unit Version 1 1F November 2012 7 408 733 7337 techserv scigene com Sc G CO nN CO Hybex
4. a few feet of the power source The unit should be positioned to have clearance along the top so that the lid can be opened completely without interference Leave at least 3 inches of clearance from the back panel for air circulation Insert one Hybex heat block See Section VII for a list of available heat blocks and Section III for how to insert and remove blocks Do not operate the system without a heat block inserted into the heating unit Plug the provided power cord into the back of the unit and then to a properly grounded outlet Use only the power cord provided Turn on power to the system using the switch on the front of the unit Version 1 1F November 2012 5 408 733 7337 techserv scigene com Sc G CO n e Hybex Microsample Incubator USER MANUAL lil USING YOUR HYBEX SYSTEM A Components Controls and Accessories Temperature Controller Used to set and observe block temperature Heats the sealed air around the block Senses the temperature of the block Heats tubes or plates without cap condensation For array hybridization 2 needed for operation For heating buffers or water Digital Thermometer For calibrating system temperature Optional accessories wy Microarray Incubation Chamber Cat 1057 38 0 2 Kit 0 00 000 000 0 D 00090 00000 V 0 d 0 0 0 CH 00 0 000 OOK Doo PAPA 00000 Heat Block for 0 2 ml Tubes or Plate Cat 1057 31 0 Heated Li
5. offset is 0 3 C then the new offset is 1 4 C 8 Press the Infinity Key ee twice to exit calibration and return to the operation display Verify that the temperature on the thermometer matches the display Your incubator is now calibrated to provide accurate temperatures between 35 and 99 C Version 1 1F November 2012 8 408 733 7337 techserv scigene com Sc G CO nN CO Hybex Microsample Incubator USER MANUAL IV MAINTAINING YOUR HYBEX SYSTEM A Powering Off Turn the power switch to the OFF position and unplug the power cord before performing any service procedure B Checking and Replacing Fuses There are two fuses located in a removable fuse block below the power cord receptacle on the back of the unit To remove fuses 1 Insert a small flat blade screwdriver into the tab recess just below the plug receptacle 2 Push down to release the fuse block 3 Slide out the fuse holder from the fuse block while holding the retaining tab out of the way 4 Gently pry out the fuses A blown fuse appears dark Always replace fuses with those of the same amperage and voltage as shown on the label below the fuse block C Cleaning Clean the metal surfaces and heated lid using a mild detergent based cleaner and wipe with a soft cloth Avoid abrasive cleaners that can scratch surfaces The bottom of the chamber is sealed and will retain spills and condensation Clean the block chamber and temperature probe fr
6. Hybex Microsample Incubator USER MANUAL Cat 1057 30 0 1057 30 2 470 Lakeside Dr Ste F Sunnyvale CA 94085 4720 USA e Tel 408 733 7337 Fax 408 733 7336 techserv scigene com Sc G CO nN CO Hybex Microsample Incubator USER MANUAL Serial Number The following serial number identifies the specific instrument you have purchased and must be referenced when requesting service A copy is affixed to the instrument Technical Service 408 733 7337 techserv scigene com Warranty SciGene warrants that the heating unit described in this manual shall be free of defects in materials and workmanship for a period of 12 months from date of delivery This warranty does not cover removable blocks or accessories In the event of a defect during the warranty period SciGene s limit of liability will be to provide replacement parts at no charge or at its sole discretion replace the product The foregoing warranty is void in the event the unit was abused or modified or used in a manner inconsistent with its intended purpose SciGene makes no other warranty expressed or implied including warranties of merchantability and fitness for a particular purpose In no event shall SciGene be liable for any direct indirect special incidental or consequential damages or for any damages resulting from loss arising out of or in connection with the sale use or performance of the product Copyright Copyright 2004 2012 SciGene Corporation A
7. ature l 2 Hold in the SET button to view the previous set temperature While holding down the SET button press the up or down arrow button until the desired set temperature is shown Release the buttons to display the actual temperature The instrument will adjust block heating until the new temperature is attained B Calibrating the Watlow SD31 Temperature Controller Re calibrating the Hybex temperature controller is recommended only if the display varies by more than one degree 1 C to a connected NIST certified digital thermometer SciGene cat 1051 52 0 To adjust the controller to achieve accurate temperatures 1 Insert a block into the Hybex incubator turn ON the instrument and set the controller to 65 C Allow 15 minutes to stabilize Using the cable provided with the digital thermometer plug one end into the blue receptacle found on the back panel of the Hybex incubator and the other end into the digital thermometer Turn on the thermometer Allow 1 minute for thermometer to stabilize The actual temperature of the block will be displayed Calculate the difference in the temperature shown on the controller and the digital thermometer For example if the controller displays 52 5 C and the digital thermometer displays 51 0 C the difference offset is 1 5 C On the temperature controller press the Infinity Key ce for Actual Temperature three seconds until OPEN appears Press the down arrow fou
8. cubator Whenever such symbols appear on instruments please observe appropriate safety measures 1 Electrical Symbols This symbol indicates that this is a protected ground terminal that must be connected to earth ground before any other electrical connections are made to the instrument CAUTION This symbol alerts you to consult this Operator s Manual for further information and to proceed with caution This symbol indicates the OFF position of the main POWER switch This symbol indicates the ON position of the main POWER switch Of 2 Non Electrical Symbols CAUTION This symbol illustrates a heat hazard Proceed with caution when working around these areas to avoid being burned by hot components CAUTION This symbol alerts you to consult this Operator s Manual for further information and to proceed with caution gt B Version 1 1F November 2012 3 408 733 7337 techserv scigene com Sc G CO nN CO Hybex Microsample Incubator USER MANUAL D Warnings Failure to comply with the following warnings that are affixed to the product can lead to possible personal injury or death This symbol on the rear of the instrument indicates the presence of the fuse box Warning For Continued Protection Against Fire Replace Only with Same Type Rating of Fuse Always disconnect the power cord before attempting to replace the fuse E Cautions Failure to comply with the following cautionary statement affixed to the pro
9. d Temperature Controller Heat Block for 0 6 ml Tubes Cat 1057 33 0 Waterbath Insert Cat 1057 35 0 Temperature Probe E Heat Block for 1 5 ml Tub eat Block for 1 5 ml Tubes D u Cat 1057 34 0 Ve Digital Thermometer Cat 1051 52 0 Heat Block for Flat Bottom Microplate Cat 1057 39 0 Version 1 1F November 2012 6 408 733 7337 techserv scigene com Sc G CO nN CO Hybex Microsample Incubator USER MANUAL B Handling Tube Blocks and the Waterbath Insert Tube heating blocks and the waterbath insert are placed and removed from the heating unit with the aid of two bolts provided that act as handles To properly install a block or insert 1 Thread the two bolts several turns into the block 2 Grasp the ends of the bolts lift the block and then carefully lower it into the unit guiding it over the temperature probe The opening in the bottom of the block should fit over the probe 3 Remove the threaded bolts before using the system Do not operate the instrument without a block roperly inserted into the heating unit C Hybex Certified Labware Hybex Certified Labware is required for guaranteed fit and performance e Blocks are designed to fit SciGene labware for optimum heating of samples e Caps remain sealed at high temperature to prevent sample loss See section VII ORDERING INFORMATION for a list of available labware Actual Temperature Set Temperature D Using the
10. duct may lead to possible personal injury This symbol indicates the potential presence of a Hot Surface Use care when working in this area to avoid being burned F Compliance European Community All instruments shipped to the European Union EU formerly known as the C s European Community have the CE label on the back of the instrument signifying that these instruments comply with the Electromagnetic Compatibility and Low Voltage Directives US and Canadian Listings All instruments meet Laboratory Equipment standards UL 61010 1 2004 R7 05 and CAN CSA C22 2 61010 1 2004 Version 1 1F November 2012 4 408 733 7337 techserv scigene com Sc G CO nN CO Hybex Microsample Incubator USER MANUAL ll UNPACKING AND SET UP A Unpacking the Heating Unit The Hybex Microsample Incubator heating base is shipped on its side within the interior carton Remove any packing material around the unit and lift the unit out taking care not to damage the hinged cover Please note that heat blocks and other accessories are packaged separately from the heating base Carefully inspect the heating unit for damage If there is evidence of damage do not discard the shipping materials since they may be needed to return the unit B Parts Provided The following items are included with the Hybex heating base within the re closable plastic bag e Power cord e User Manual C Installation Place the unit on a level surface within
11. equently to avoid build up of residue that can interfere with the heat control system Version 1 1F November 2012 9 408 733 7337 techserv scigene com Sc G CO nN e Hybex Microsample Incubator USER MANUAL V TROUBLESHOOTING symptom IL e IL S Power switch light does not turn Blown fuse s Replace fuse s on back of unit on beneath power cord receptacle VI SPECIFICATIONS Cat 1057 30 0 115V AC 50 60 Hz 3A Cat 1057 30 2 230V AC 50 60 Hz 1 6A Outside cover closed H x W x D 10x6x12 inches 21x15x30 cm Instrument without block 6 Ibs 3 kg Performance and Controls Version 1 1F November 2012 10 408 733 7337 techserv scigene com Sc G CO nN CO Hybex Microsample Incubator USER MANUAL Vil ORDERING INFORMATION vee enon 1057 38 0 Hybex Microarray Chamber Kit Includes two chambers four slide racks EA two handles and assembly tools 1051 52 0 Digital thermometer Includes cable and NIST certificate 2011 10 0 96 Well PCR Plate skirted 10 cs Seal with PCR Plate Sealing Foil 10 CS cat 2011 20 0 using PCR Plate Roller cat 2011 21 0 Use at temperatures below 80 C 2011 11 0 96 Well PCR Plate skirted 50 cs Seal with PCR Plate Sealing Foil 5x10 CS cat 2011 20 0 using PCR Plate Roller cat 2011 21 0 Use at temperatures below 80 C 2011 12 0 96 Well PCR Plate non skirted 10 cs Seal with 8 Strip Flat Caps 10 CS cat 2011 22 0 using PCR Plate Roller cat
12. ll rights reserved SciGene and Hybex are trademarks of SciGene Corporation Sunnyvale CA All other trademarks used in this manual are the property of their respective owners Version 1 1F November 2012 1 408 733 7337 techserv scigene com Sc G CO nN CO Hybex Microsample Incubator USER MANUAL Table of Contents I SAFETY NOTICES ssssirissrnisesseiisi esni S EE aaa 3 A WSS Eege 3 Be E a EEN 3 EE ana CONVENON srecan ar a E 3 D AN an E 4 E BoD e e E A E A E A A E A E A A stees 4 F CM AG EE 4 il UNPACKING AND SET UP sssiicsscccnensasssvessscsadinsssans ances ediuensvsavsdiiseusldaaunssnensscoiidnedeseneesssadeeceesesdawessecoeweses 5 A Unpackne rye CN leed 5 Be NUS PROVIGIL ME 5 NS RON E 5 ie USING YOUR IY BEX CN UE 6 A Components Controls ANd Accessories ccccccccsssecccceesseccecauesececceeuseccesaaaseceeesueseceeessuaseceesaaneesauaaeceesaaaeeeeeeeas 6 B Handling Tube Blocks and the Waterbath Insert ccccccccsssecccccesececcceesececceeeseceeseseeaeceesseneeeeesaaeeesauaaeeeeseeaaes 7 C Hybex e eg 0 EE 7 D Using the Temperature tree dee eege dee deed EE ege eege 7 E Calibrating the Temperature Controller 7 IV MAINTAINING YOUR FY BEX SYSTEM wscsssescssscccecsocncsstnnvieccanesscncasanvsaseneiseieasentuneacedanssacesseacesnesauesiesoene 9 Ae POW E EE 9 B Checking and Replacing TE 9 SME E E 9 Glen el e Lie TIN EE 10 Vi ee e al VE 10 Vil ORDERING INFORMATION scssvsciccscssussencssoasenscesmben
13. r times until Cal appears Press and hold the SET key The existing offset value between the controller and digital thermometer is displayed Set Button Up Down Arrows Press and hold the SET key and use the up down arrows to SEHR adjust the offset value to the temperature difference calculated in Step 4 For example if the controller shows a temperature 1 5 C lower than the thermometer adjust the offset by adding 1 5 to the value shown Press the Infinity Key ee to exit calibration and return to the operation display Verify that the temperature on the thermometer matches the display Your incubator is now calibrated to provide accurate temperatures between 35 and 99 C Version 1 1F November 2012 12 408 733 7337 techserv scigene com Sc G CO nN CO Hybex Microsample Incubator USER MANUAL IX DECLARATION OF CONFORMITY Hybex Microarray and Microsample Incubation System SciGene 470 Lakeside Drive Suite F Sunnyvale CA 94085 4720 USA Declares that the above referenced product s meets the essential requirements of the following European Union Directives by using the relevant standards shown below to indicate compliance EMC Directive 89 336 EEC Standards EN 61326 1 1997 Electrical equipment for measurement control and laboratory use to include EN 61000 4 2 2001 A1 1998 A2 2001 A3 2003 EN 61000 4 3 2001 EN 61000 4 4 2001 EN 61000 4 6 2003 LVD Directive 2006 95 EC EN 61010 1 2004 Safety
14. requirements for measurement control and laboratory use 2 Edition Part 1 General requirements Terry Gill Sunnyvale California USA Name of Authorized Representative Place of Issue Director of Product Manufacturing May 6 2009 Title of Authorized Representative Date of Issue IL PAM Signature of Authorized Representative Version 1 1F November 2012 13 408 733 7337 techserv scigene com
15. sscetsesiidassnnsdasecnsadedeverenseucseeienssaeesenuessiseduavemseccesseus 11 Vill APPENDIX A Using and Calibrating a Watlow SD31 Controller ccscsecsccsccecsccsccccsccscescnees 12 A Using the Watlow SD31 Temperature Controller cccccccsssssccccceeseccecceesececcceeaseeceeseeusecesseaaeceesaeaeeessuaaeeeeeas 12 B Calibrating the Watlow SD31 Temperature Controller ccccccccsssseccccssesseccceceesececeseeeceeseaeeceessaesseeeseeeesseees 12 IX DECLARATION OF CONFORMITY WE 13 Version 1 1F November 2012 2 408 733 7337 techserv scigene com Sc G CO nN CO Hybex Microsample Incubator USER MANUAL A SAFETY NOTICES Intended Use The Hybex Microsample Incubator is intended for the heating and incubation of laboratory samples The instrument should only be used according to the instructions provided in this manual If the equipment is used in a manner not specified by the manufacturer SciGene the protection provided by the equipment may be impaired Instrument Safety Before operating the instrument read the information in this section concerning hazards and potential hazards Ensure that anyone involved with the instrument s operation is instructed in both general safety practices for laboratories and specific safety practices for the instrument Symbols and Conventions The following chart is an illustrated glossary of the electrical symbols that are used on the Hybex Microsample In
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