BAT Agar 2.04719.782 User Manual en 2.1
Contents
1. User manual BAT Agar Product No 2 04719 782 page 1 3 1 Application Nutrient media for the detection of Alicyclobacillus spp in the beverage and fruit compound industry BAT Agar pH 4 0 0 1 is an agar which in combination with the BAT Broth and Guaiacol Detection Kit is a fast reliable sensitive and cost efficient method for analysis of Alicyclobacillus even in complex products and is compliant to the IFU Method Method on Detection of taint producing Alicyclobacillus in Fruit J uices IFU Method No 12 Alicyclobacillus can spoil beverages by creating an off flavour Its spores can survive normal pasteurisation treatments and may be activated and grow in favourable conditions after a few weeks Use the BAT Agar as a nutrient media for bacterial smear 2 Handling Required Material Double boiler temp 95 C 200 F BAT Broth Product No 2 04719 782 for cold filled ready to use drinks PCA Plate Count Agar e g DEV Nutrient Agar Product No 2 04726 782 microbiological workbench Petri dish inoculation loop for bacterial smear incubator Implementation Handle the samples under sterile conditions to avoid secondary contamination Liquefy the nutrient media BAT Agar and Plate Count Agar in a double boiler at max 95 C 200 F Cool it slowly for use to approx 45 C 113 F Pour the nutrient media into Petri dishes keep the different Agars separated to a height of 3 4 mm Cool the dish until the Agar sol2. 24 Tel 983 251 143 637 596 017 47010 VALLADOLID Version 2 1 28 November 2011 WWW analisisavanzados com this version replaces all previous versions
3. Agar pH 4 and PCA pH 7 then spread with a bent rod place plates in plastic bags as protection from drying incubation at 45 C 1 C 1 3 days growth of rods on PCA Alicyclobacillus negativ growth of Alicyclobacillus positive Alicycibacilius spp at pH 7 not Alicyclobacillus negative possible Fig 1 Evaluation of BAT Agar Version 2 1 28 November 2011 this version replaces all previous versions User manual BAT Agar Product No 2 04719 782 page 3 3 3 Storage and packaging Information Packaging and Content unit Cardboard Box 9x250mI bottles unit size Box approx 22cm x 22cm x 18cm 8 7in x 8 7in x 7 1Lin unit gross weight Box approx 4 1 kg 9 Ibs Storage Store at 4 8 C 40 45 F according to product specification Store under dry and dark conditions Do not freeze product Waste Disposal No dangerous good No hazardous material Please consider your local waste regulations Uninoculated agar can be disposed of with normal laboratory waste Inoculated and incubated samples are to be sterilized before disposal at a temperature of 250 F 121 C and a holding time of 20 minutes Warnings Don t cook or freeze the nutrient media Heating can restrain its functionality Always wear protective equipment when handling with hot media Never firmly close a fermenting sample Bottle may explode SISTEMAS AVANZADOS DE ANALISIS S L CIF B 47700026 C Cardenal Torquemada
4. idifies For cold filled ready to use drinks and compounds Pour 0 1ml of the enriched bacterial culture each See BAT Broth specifications onto the Surface of the pre poured BAT Agar pH 4 and a Plate Count Agar pH 7 Spread with a Sterile inoculation loop For hot filled ready to use drinks Pre incubate the product in the original package at 45 C 113 F for 7 days to obtain a bacterial enrichment Pour 0 1ml of the enriched product onto the surface of the pre poured BAT Agar pH 4 anda Plate Count Agar pH 7 Spread with a sterile inoculation loop Incubation Place the test sample stored in a plastic bag to avoid drying in an incubator for max 3 days at a temperature of 45 C 113 F Breed in aerobic conditions Version 2 1 28 November 2011 this version replaces all previous versions User manual BAT Agar Product No 2 04719 782 page 2 3 Evaluation Evaluate the samples A lack of bacterial growth on the BAT Agar indicates a negative result for Alicyclobacillus With a positive growth of rods on BAT Agar please check the PCA for further results If there is no visible bacterial growth on the PCA the result for Alicyclobacillus is positive If you find a growth of rods on the Plate Count Agar the result is Alicyclobacillus negative The growth of Alicyclobacillus spp at pH7 is not possible fig 1 growth in BAT Broth no pour ca 0 1 ml of the bacterial culture onto the surface of pre poured BAT
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