O&M Manual for NuAire C Model Benchtop Centrifuges
Contents
1. 25 7 6 Checks and tests on accessories LR 25 Isle TOG Manz LA LES AE A ee RTT IDEO TUUM 25 8 TROUBLESHOOTING WA mmm ne enne nennen nnn na nnn n 26 9 SPARE PART prt 28 I0 DS PO AL a A 31 11 CERTIFICATE OF DECONTAMINATION RETURNS FORM 31 12 LOGBOOK ss mmm nenne nemen rne nnn anna nn nnns 34 MEL 0c aa a ae ma su ee a ai un 35 OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 4 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com AWEL CENTRI FUGES User Manual C 12 20 20 R 48 48 R Models 1 WARRANTY AWEL centrifuges are warranted for two years by NuAire This warranty applies if The centrifuge is used conforming to the instructions in the user manual The electrical installation at the site of the centrifuge conforms to the IEC recommendations AWEL International declines all responsibility for damage caused by use not conforming to instructions failure to carry out maintenance operations and any unauthorized modification Conforming to instructions for use comprises respecting instructions in the user manual and a carrying out of inspections and maintenance VAN THI S USER MANUAL BEFORE THE FIRST OPERATI ON OF THE MACHINE 2 GENERAL 2 1 Environment See Section 4 3 1 Environment 2 2 Technical specifications C 12 model Maximum capacity 4 x 125 ml swing out rot2. Kit bo te outis o 40000009 LockSwithkitt Kit Switch serrure 6 40000010 Decontamination kt Kitded contamiaton 40000012 Innerblackcap Bouchon int rieur noir 8 40000013 Connexion kit R Link Kit de connexion R Link 40000014 Kit of lid amp arrow stickers for C 20 R amp MF Kit tiquettes Couvercle amp Fl che C20 R amp MF 40000015 Knob JMote q O PS PE 40010001 Stickerskitfor 12 C 48 Kit tiquettes C 12 7 C48 o O O DR 40100004 Fuses kit 230V 50Hz Tkitfusibles230V 50Hz 40100005 Fuses kit 120V 60Hz jKitfusbles 120V 60Hz Motoreducer lock 120V 60Hz Kit Serrure sans motor ducteur RS RR dA ee OS AE O O EE Refrigeration group 230V 50Hz Groupe froid 230V S0OHz 40102002 Refrigeration group 120V 60Hz Groupe froid 120V 60Hz 40102003 Fan motor kit 230V 50Hz Kitventiiateur230V 50Hz 1 0 40102004 Fan motor kit 120V 60Hz Kit ventilateur 120v 60Hz 40102005 Compressor kit 230V 50Hz Kitcompresseur230V 50OHz 40102006 Compressorkit 120 60Hz Kitcompreseeur120V 60H2 RR c s qgsgsg g g su OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 29 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 5
3. capacity 15 x 5 7ml BD collection tubes insert Tod id AW I Q4g sT Set of 4 inserts capacity 19 x 5 7ml Terumo me 3668 collection tubes insert Set of 4 inserts AW 1 048 10 capacity 12 x 10ml collection tubes insert ee none Set of 4 inserts AW I 048 15 capacity 7 x 15ml conical bottom tubes insert id 0 Set of 4 inserts AW 1 048 53 capacity 3 x 50ml conical bottom tubes insert ans 3003 Set of 4 inserts AW 1 048 54 capacity 4 x 50ml conical bottom tubes insert moun aie AW 048 22 Set of 4 inserts capacity 8 x conical bottom 164 4500 3713 j tubes dia 22mm insert Abo je de D 120 140 4400 2590 3030 32x15 ml capacity 32 tubes 15ml 2 5 Packing list e Centrifuge C model e Power cord mains cable e User manual e Allen wrench for manual unlocking of the cover e Pipe wrench for unlocking accessories e Food quality container of grease e Quick Start Guide OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 10 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com Safety rules The operator must respect the following precautions o Do not attempt to open the cover while the display indicates that the end of the centrifugation run has not been reached o Do not attempt to override the lid locking system during operation of the centrifuge See section 3 3 2 o Use only an electrical
4. www nuaire com 8 TROUBLESHOOTI NG Centrifuge Poor loading of Err 001 Imbalance rotating accessories unbalanced The lid has been unlocked manually Wait until the complete stop during rotation Switch off the centrifuge for several abnormal and seconds then switch it on again Lid OPEn Lid open dangerous handling press the lock unlock key to open the The locked position lid close the lid again and restart the detectors are centrifugation defective or badly If the problem persists contact the adjusted AWEL service department The set temperature is too high Check the conditions of use set The ambient temperature below 60 C and ambient temperature is too temperature below or equal to 25 C high Braking to a complete Once the correct conditions of use have The cooling system is stop been checked and or re established faulty restart the centrifugation The temperature If the problem persists contact the detector is faulty or AWEL service department badly connected The ambient Reload the rotating accessory and Braking to a complete restart the centrifugation An imbalance stop higher than or equal to 15 g is forbidden Power to the motor is cut immediately and the rotor turns freely until it stops Err 003 Refrigerated centrifuge Bowl temperature too high Check the ambient temperature it temperature Is too must be below or equal to 25 C Motor nat Let the centrifuge c
5. Continuous centrifugation Hold mode Select the value Start the run by pressing the Start buttonk 2 The centrifuge runs until the Stop button IG Is pressed 6 1 3 Temperature 6 1 3 1 Pre cooling Press the Ny key to start a pre cool cycle 2000 rpm 15 minutes 4 C 6 1 3 2 Programming Temperature Press the 6 key until the display of the temperature goes to programming mode only the temperature is displayed in violet Select the required value with the help of the knob while in programming mode The new value is automatically saved after 5 seconds or if the key for another parameter is pressed 6 1 3 3 Instantaneous temperature reading It is possible to read the instantaneous temperature in the lower part of the bowl when the rotor is stopped Keep pressed the 6 key The value is displayed in blue 6 2 Creating Modification of a program 6 2 1 Creating a program After switching on the centrifuge displays the last program used Select the program number by pressing the key short press The program numbers will appear sequentially when turning the knob Stop turning the knob once the required number is displayed The word prog stops flashing after five seconds Enter the values for the different parameters as indicated above To memorize the program hold the key pressed in The display becomes green for a few seconds to confirm that the values have been saved 6 2 2 Locking a program The prog
6. 1 1 1 Centrifuge acceleration formula 21 6 1 1 2 Selection of Speed Radius Relative Centrifugal Force 21 6 1 1 3 Programming the speed rpm and centrifugal force RCF ssesee 21 6 1 2 TIME ir a tiv tianel cen 22 5 1 2 1 Programming tne TIM core eva E bere nda a ona ca 22 6 1 2 2 Continuous centrifugation Hold mode 22 O13 Temperature onci d RNIPEUM rx I ERRORI Ad I OUI PrFIVI I RN RREpERKUN DIEN VERPPEH EM 22 6 1 3 1 Pre cooling DR 22 6 1 3 2 Programming Temperature 22 6 1 3 3 Instantaneous temperature reading 22 6 2 Creating Modification of a program 22 0 2 L Cbald s DOC a ea cn nt 22 Diz LOCAS a DOOF tct iniatar TP DUO DA REIHEN USD TS 22 61255 PO aN S CIRCULO ii 23 6 2 4 Modification of a program mmm nenne 23 Oo USING DOO AIM iris 23 7 CARE AND MAINTENANCE visitation rater cod va elt cd t dc 24 7 1 Daily care and cleaning 24 7 1 1 External body 24 TIE BOWI ANO ACCESSOTIOS a 2 dense sn near dada ite nin ns 24 7 2 Weekly care and CI ANDO ee 24 EE AUS OT ACCESSOTIES IU TULIT 24 7 4 Action to be taken in the case of broken glass or accidental spillage 24 7 4 1 Material presenting no toxic biological or radioactive risk 24 7 4 2 Material presenting a biological risk 24 7 4 3 Material presenting a radioactive risk 24 7 4 4 Material presenting a toxic risk 25 7 5 Sterilization and disinfection of the centrifuge and its accessories
7. 20 R Version 120V 60Hz AW C 020 CR Packaging HxWxD Recyclable 18 x 24 x 25 5 8 460 x 610 x 650 mm packaging C 20 R 18 x 24 x 35 460 x 610 x 890 mm Weight gross net 110 123 Ibs 50 56 kg C 20 R 154 174 lbs 70 79 kg OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 6 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com C 48 amp C 48 R models 20 C to 60 C refrigerated version External dimensions HxWxD C48 model 11 x 16 1 2 x 20 1 2 280 x 420 x 520 mm C 48 R model 11 x 16 1 2 x 28 280 x 420 x 710 mm 230V 10 50 Hz 120 V 10 60 Hz C48 R Power supply Model 008 UE Version 230 V 120 V 230 V 120 V remna Wm mu Maximum Noise 62 dB A 60 dB A Model Description Catalog Numbers C48 Version 230V 50 60Hz AW C 048 CVE C 48 R Version 230V 50Hz AW C 048 CRE C 48 R Version 220V 60Hz AW C 048 CRF C 48 Version 120V 60Hz AW C 048 CV C 48 R Version 120V 60Hz AW C 048 CR Packaging HxWxD Recyclable 18 x 24 x 25 5 8 460 x 610 x 650 mm packaging C 48 R 18 x 24 x 35 460 x 610 x 890 mm C 48 110 123 lbs 50 56 kg Weight gross net C 48 R 154 174 Ibs 70 79 kg OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 7 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S
8. control is possible 9 Pre Cool Refrigerated models 10 Access to the numerotation of the program G1 Access to the number of revolutions minute Access to the duration of the cycle 13 Access to the temperature control OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 20 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 6 PROGRAMMI NG 6 1 Parameter values Parameter values are entered directly with the help of the specific key and the value adjustment knob 6 1 1 Speed rpm Relative Centrifugal Force RCF Radius 6 1 1 1 Centrifuge acceleration formula 2 2 RCF Nr 9 10 g N Rotation speed in rotation minute rpm r Radius in mm g 9 81 m s F Centrifuge acceleration or g number Approximation 2 F 1 118xrx A 1000 To calculate Speed from Relative Centrifugal Force N 1000 x 1 118 xr 6 1 1 2 Selection of Speed Radius Relative Centrifugal Force Repeated pressing on the key enables the speed centrifugation radius and relative centrifugal force to be displayed successively 6 1 1 3 Programming the speed rpm and centrifugal force RCF Select the speed rpm or relative centrifugal force RCF using the key Press the key firmly until the display goes to programming mode only the rpm value appears in a violet color Repeated pressing on the key enables
9. the lid eenm cen erre nen enre menta 12 3 3 2 Manual unlocking of the WC sos non a a qteud Gat ment Raadtenont aaa 13 4 INSTALLATION AND SETTINGUPNN mmm 14 4 1 Unpacking the centrifuge 14 2 2 LOGO NO saias EE E IR HMM PHI FERAI ESA 14 4 3 Installation EP 14 A oka ENIO SO sp T 14 nz TOWEL SNI POA nr 15 4 3 3 Place of installation iii iara ga CE SUYA JCRY ERA TOMICHLX UT FA EAR E CHA IS 15 o ee 16 4 4 1 Switching on the centrifuge ss nennen 16 20 2 LA ODENIN eee ee RE DIS Tin MP OON FCR EEPtiLI PEKIN CMM UMP 16 AoA 3 Installlligd TOOT CR assi SA 16 2 09 1 ROLO MOUNN agr PEN DRE TERUS 16 2 4 5 2 RoLor dISmiOU DI D 2 inen en iva DO bcp ba er Deor e m bre eer EEE on d d 17 EA LOCO HIS FOLOE ne VEDI I IU DRIVER DPI PS 17 22 5 CIOSINO THO WG scis aen quia taorBrtu dd mU VE ERI IR DII ETAPA d rM reri 18 4 4 6 Detection of locking failure 19 4 4 7 Automatic speed limitation 19 4 4 8 Detection of end of run AWELight sse 19 5 CONTROLIPANEL sociais dO a in 20 6 PROGRAMMES ae ea aa at me mano 21 6l Parameter Valu lisa nn tease Eden cra add 21 OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 3 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 6 1 1 Speed rpm Relative Centrifugal Force RCF Radius eee 21 6
10. 020 5C capacity 6 x 5 7ml collection tubes insert Tes is aw 1020 5B Set of 4 inserts capacity 7 x 5 7ml BD amp 2405 in Greiner collection tubes insert AW 1 020 5T Set of 4 inserts capacity 9 x 5 7ml Terumo 158 4400 3420 j collection tubes insert Set of 4 inserts AW 1 020 10 capacity 6 x 10ml collection tubes insert biis E Set of 4 inserts AW I 020 11 camacity 7 x 10ml BD collection tubes insert idis 2 Set of 4 inserts AW 1 020 15 capacity 3 x 15ml conical bottom tubes insert 4400 3500 Set of 4 inserts AW 1 020 50 capacity 1 x 50ml conical bottom tubes insert TUM din AW 1 020 22 Set of 4 inserts capacity 4 x conical bottom 158 4400 3420 j tubes dia 22mm insert AW R 020 cA 220 32 15 Angle rotor 120 140 4400 2590 3030 32x15 ml capacity 32 tubes 15ml OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 9 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com C 48 amp C 48 R models P Max Speed RCF Max cat Description tm qepm Gg volt AW R 048 SO SO 400 Swing out rotor AW B 048 RB N 400S Set of 4 buckets 169 4500 3820 4x250 ml AW L 048 RB BL 400S Set of 4 sealing lids Set of 4 inserts AW I 048 02 capacity 13 x 1 5ml microtubes insert idis di Set of 4 inserts AW I 048 5C capacity 14 x 5 7ml collection tubes insert 1908 ad Set of 4 inserts AW I 048 58
11. 5447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com Part English Designation D signation en Fran ais Item 40110001 C12 Display board kit Kit Carte afficheur C12 Eq ooo RS PS DS ul RS y DS pq IE MF 20 AWELock kit Kit AWELock MF 20 MF 48 AWELock kit Kit AWELock MF 48 Depending on model Selon mod le Du 40110002 C20 R Display board kit Kit Carte afficheur C20 R OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 30 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 10 DISPOSAL Information concerning the disposal of electrical and electronic equipment in the European Union Electrical equipment is governed by national rules based on European Directive 2002 96 CE relating to electrical and electronic equipment waste EEEW To conform to this directive all equipment delivered after 13 August 2005 in the business to the business sector to which this centrifuge belongs category 8 Medical Devices must not be disposed of with domestic waste To facilitate their identification the equipment concerned is provided with the following symbol The regulations related to the disposal of such waste vary from one country to another within the EU and we invite you to contact your local supplier 11 CERTIFI CATE OF DECONTAM
12. A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 2 3 Dimensions Positions of the feet e Front View 420 e Side View Ventilated model 345 181 526 Refrigerated Model OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 8 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 2 4 Accessory table C 12 model E Max Speed RCF Max AW R 012 SO SO 125 Swing out rotor AW B 012 RB N 125 Set of 4 buckets 162 3000 4x85 ml AW L 016 RB BL 125 Set of 4 sealing lids Set of 4 inserts AW 1 012 05 capacity 4 x 5 7ml collection tubes insert 4000 2862 Set of 4 inserts AW I 012 10 capacity 4 x 10ml collection tubes insert dos iic Set of 4 inserts nio capacity 1 x 15ml conical bottom tube insert idus ep Set of 4 inserts AW I 012 50 capacity 1 x 50ml conical bottom tube insert idus dis AW R 012 CA A20 32 15 Angle rotor capacity 32 tubes 15ml 120 140 4000 2140 2500 32x15 ml C 20 amp C 20 R models o Max Speed RCF Max AW R 020 SO SO 200 Swing out rotor AW B 020 RB N 200 Set of 4 buckets 162 4400 3500 4x200 ml AW L 016 RB BL 200 Set of 4 sealing lids Set of 4 inserts AW 1 020 02 capacity 7 x 1 5ml microtubes insert iiid is Set of 4 inserts AW I 020 5P capacity 12 x 5ml tubes insert 2000 iia Set of 4 inserts AW I
13. I NATI ON RETURNS FORM Equipment will not be accepted without a returns form and the certificate of decontamination which must be fixed to the outside of the packaging In the absence of a certificate of decontamination the instrument will be decontaminated by an external organization and the operation will be invoiced to the sender OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 31 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com CERTI FI CATE OF DECONTAMI NATI ON the undersigned First name ET 51010117120 CS ia ren BS aii COMPANY asi ceanesuenens a 6 6 2 OO n Post Code eeseeseenenmnn en UAR Certify that the equipment described below 5 pc no c Model a Serial Number eeeeeeseemmennt Has been decontaminated according to the following protocol Decontaminating agent ienes MeO 22 eoteatarnnenanonsemaceemeas Contact time iss Carried out by eeeeee mH Checked by Date a Date Signature OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 32 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com RETURN FORM Dates Aue entr EN VdME CA E E IS COMECE ss NI TAC Er raTa KOOS m RO E uentansattecniass P eens az pceai
14. NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com Tighten the locking nut with the supplied wrench o Inthe counterclockwise direction for C 12 amp C 48 48 R models Rotor mounting nut for C 12 amp C 48 48 R models o In the clockwise direction for C 20 20 R models de a ITE J r m f 1 j 1 J e m i A a j a Rotor mounting nut for C 20 R models Rotor mounting 4 4 3 2 Rotor dismounting Unlock the rotor from the shaft by using the pipe wrench delivered with the centrifuge e Turn clockwise for C 12 amp C 48 48 R models e Turn counterclockwise for C 20 20 R models 4 4 4 Loading the rotor For swing out rotors always puts all the buckets and inserts even empty in their dedicated location Rotors must be balanced for load about a symmetrical axis the contents of each pocket in the rotor or insert must be balanced in weight with that of the diametrically opposite position The tubes or bottles must be balanced by pairs that are placed opposite each other n the case of an odd number of tubes balance the last one by adding a tube filled with water of the same total weight Bucket static balancing o O y Y O v OO OO ole ove O e O 9 V OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 17 of 35 N
15. a aioe dm eet asians ated eearesyudeees onsen aout EE NANNA sil io Roc Didar E dai scan didad cas dude asse td Equipment returned mmm Brana 5 5 OAE Ae PR IURE MOCE E A sient LL a am in de Under Guarantee YES O NO O CN ni RedSON TOR PCLUNUING M Mc 0M0204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 33 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 12 LOGBOOK Example of table to trace the use of the centrifuge and its accessories Date Operator rpm RCF Time T C Rotor Incidents RS RE OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 34 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 13 NOMOGRAM 50 20 000 45 15 000 40 10 000 35 30 000 20 000 30 10 000 oe 25 5 000 4 000 E 20 2000 3 000 E pe 18 1 000 A A 27 500 lt A adi 14 pe afd 200 12 a 3 a 1 000 a p p 100 10 AAA p 50 9 A e 500 8 20 10 6 4 E 200 Radius RCF Speed cm xg rpm Example The relative centrifugal force RCF at a radial distance of 8cm from the center of centrifuge spindle when operating at a speed of 2500 rpm may be determined by placing a straight edge on the nomogram co
16. atible with the rotating accessory centrifuge Check that the accessory is correctly loaded balanced Restart the centrifugation once these checks have been carried out If the problem persists contact the AWEL service department Important Do not attempt to refit by yourself a magnet that has become detached from the rotating accessory a special type of glue must be used and the operation carried out by an engineer trained by AWEL Missing magnet Rotor turns freely until Err 006 Irregular it stops impossible to count speed open the lid before the down signal end of the countdown Major imbalance of the 900s 15mn rotating assembly Err 007 Notused Not used Not used Not used Try to open or close the Lid again check that nothing is inserted in the Impossible to start the right locking system hole Switch the centrifuge till the unit OFF wait a few seconds and problem is solved switch it ON again If the problem persists contact he AWEL service department Wait 1mn and try to start the centrifuge again if Err 09 is still displayed check that the centrifuge is The centrifuge is not properly properly installed level installed the centrifuge has If leveling is not correct turn the unit AWELine been shocked moved while OFF adjust its level turn the unit ON error powered or the previous run and wait 1mn before starting the stops because of a high centrifuge again imbalance If Err 09 is still ther
17. be carried out by a person trained in the use of the centrifuge and understanding the mechanical risks Risks of wounding if the operator attempts to brake the rotor manually during rotation Risk of the projection of aerosols in the case of broken tubes within the centrifugation chamber In the case of a power outage power cut wait 15 minutes before attempting to unlock the lid manually the rotating rotor will decelerate without braking Equipment Allen wrench provided e Put the main switch in position O e Insert the unlocking device in the hole situated on the lower right side of the centrifuge punching the sticker e Connect the wrench and turn it clockwise the closing mechanism will unlock the lid that will lift slightly e Lift the lid manually amp key to reinitialize the After a manual unlocking the message Press Lid Button is displayed Press the locking device Contact an AWEL certified technician for the locking device check and the replacement of the sticker OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 13 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 4 INSTALLATI ON AND SETTI NG UP Transporting the equipment over a long distance must be done by mechanized means handled by personnel trained in its use 4 1 Unpacking the centrifuge Remove the centrifu
18. ds except those recommended here above users should check with AWEL International that the proposed method will not damage the equipment 7 6 Checks and tests on accessories These checks help in providing operator safety All accessories Carry out regularly at least once per month a visual inspection of rotors and accessories Check for traces of corrosion wear and surface faults scratches cracks Replace the accessory if there is a significant fault Sealed accessories Check the airtight seals and replace them after they have been autoclaved 10 times Check the sealing lids Replace them if the color has changed they have become opaque or if there are significant scratches 7 7 Yearly maintenance It is recommended to have a functional check Greasing of the motor shaft of the locking device readability of the stickers a security check Continuity of the grounding earthing locking and a performance check of the centrifuge at least once a year Check that the ground earth continuity of the machine is still properly ensured by the power cord and the wall outlet to which it is connected No access to the inside the centrifuge is authorized Maintenance is carried out by an AWEL certificated technician OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 25 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352
19. e check that no other appliances induces vibrations on the table If not contact AWEL service department Wait for full stop if centrifuge was rotating Turn it OFF wait a few seconds turn it ON Try to start the cycle If the problem persists contact AWEL service department Lid Switch The 2 Lid switches are seen Err 008 error closed at the same time Impossible to start the centrifuge till the problem is solved Err 009 It is not possible to Err 010 communicate with the Lid Error Stop board Lid board Braking to a complete OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 27 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 9 SPARE PARTS Exploded view of an AWEL centrifuge Rotor Locking devices AWELock device C series device C 12 C 48 R C series device C 20 R 21 NN AWELock C 12 C48 C 48 R models C 20 C 20 R models OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 28 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com Part English Designation D signation en Fran ais Item 40000004 Pot of Food Quality Grease Potdegraissedequalit alimentare 40000006 Toolboxkt amp
20. force In particular they enable biological samples to be prepared and treated before analysis within the framework of in vitro diagnostics Centrifuges are designed for interior use by personnel trained to use them 2 6 1 Practical notes on centrifugation Check that the centrifuge is stable and horizontal Check that the rotor is correctly fitted before starting a centrifugation run Check that tubes can resist the maximum centrifugal force applied See section 2 4 Accessory tables Check that tubes are in good condition eliminate damaged tubes During operation the centrifuge must not be moved or knocked Use only the rotors and accessories authorized by the manufacturer See section 2 4 Accessory tables Balance the rotor For swing out rotors always puts all the buckets and inserts even empty in their dedicated location Respect the maximum density of 1 2 g ml particularly when using maximum speed Limit the volume if the density is exceeded 2 6 2 Actions forbidden during centrifugation It is forbidden to centrifuge the following material Inflammable or explosive material Material that could react chemically with sufficient force to create danger The centrifuge is not designed to operate in an explosive environment It is forbidden to centrifuge toxic or radioactive material or material contaminated by pathogenic microorganisms in containers that are not sealed against aerosols and if necessa
21. ge from its packaging by lifting it holding the lower part Beware refrigerated models require two persons for this operation Remove the other items from the packaging power cable manual tools Check that nothing remains in the packaging before disposing of it The packaging is made of card that can be recycled and should be treated in the appropriate way 4 2 Load lifting 2 people are necessary to lift and manipulate the centrifuge WARNING Manipulate the centrifuge handling underneath and not the front panel 4 3 Installation 4 3 1 Environment Avoid corrosive high humidity atmosphere Avoid direct sunlight that could result in heating of the instrument WARNI NG The centrifuge is not designed to operate in an explosive environment Environmental conditions To be used inside Standard operating temperature 25 C Maximum relative humidity of 8096 for temperatures up to 31 C decreasing linearly to 50 percent relative humidity at 40 C Maximum altitude 2000 m Maximum performance is assured within the temperature range 15 C to 25 C Electrical environment Installation category 2 Pollution degree 2 EMC Performance Conforms to EN61326 1 ED 2006 concerning Emissions and Immunity OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 14 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 ww
22. ly 7 3 Storage of accessories Never leave a wet or moist rotor on a metallic surface even stainless steel risk of corrosion Unused rotors and accessories should be placed on a non metallic grid with pockets openings pointing downwards in order that any liquid or condensate can drain out Take care to clean and dry rotors that will be stored for weeks or months before use Cover them with a fine layer of silicone grease using a spray Equally rotors stored in a cold room must be protected against humidity by a layer of silicone grease 7 4 Action to be taken in the case of broken glass or accidental spillage Before any intervention switch off and unplug the centrifuge The user is responsible for the decontamination in case of spilling contaminated liquid in the centrifuge 7 4 1 Material presenting no toxic biological or radioactive risk Wear protective gloves to avoid any risk of injury and a mask in case of airborne contaminants Dismount the rotor Remove all debris and mop up spillages using compresses or absorbent paper Deal with all waste using the correct means for material that can stab cut or slice 7 4 2 Material presenting a biological risk Transport the sealed accessory rotor bucket together with its sealed lid to the inside of a microbiological safety cabinet Open it and dispose of the contents into an autoclave bag Clean and rinse the accessory in the safety cabinet and place it in the second autocla
23. nnecting the 8cm point on the Radius Scale on the left with the 2500 rpm point on the Speed Scale on the right The point of intersection on the Relative Centrifugal Force Scale in the middle or 550g is the relative centrifugal force OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 35 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com
24. ool for h mesure e motor Braking to a complete Once the correct conditions of use have Ra temperature detector stop been checked and or re established J is faulty or badly restart the centrifugation connected If the problem persists contact the The motor is damaged AWEL service department Rotating accessory Check that the loading of the rotating jammed in the bowl accessory does not exceed its specifications and that no obstacle to rotation has become accidentally Rotating accessory lodged in the bowl incompatible with the Rotor turns freely until Restart the centrifugation once these centrifuge it stops impossible to checks have been carried out If the Defective power board open the lid before the problem persists contact the AWEL cabling or motor end of the countdown service department 30s Important Do not attempt to refit by yourself a magnet that has become Missing magnet on a detached from the rotating accessory a Err 004 Excessive loading Err 005 count down No speed signal rotating accessory special type of glue must be used and the operation carried out by an engineer trained by AWEL OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 26 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com Rotating accessory Check on the state and the type of a incomp
25. or Maximum speed 4 000 rpm swing out rotor Maximum RCF 3000 x g swing out rotor Timer range Omin 00s to 9h 59min and Hold External dimensions HxWxD 280 x 420 x 520 mm 230 V 10 50 Hz 120 V 10 60Hz Version 230 V 120 V Power supply Dissipated Heat 1194 BTU hr 1194 BTU hr Model Description Catalog Numbers C12 Version 230V 50Hz AW C 012 CVE C12 Version 120V 60Hz AW C 012 CV Packaging HxWxD Recyclable packaging 18 x 24 x 25 5 8 460 x 610 x 650 mm Weight gross net 110 123 lbs 50 56 kg OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 5 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com C 20 amp C 20 R models Maximum capacity 4 x 200 ml swing out rotor Maximum speed 4 400 rpm swing out rotor Maximum RCF 3 500 xg swing out rotor Temperature Display 20 C to 60 C refrigerated version Timer range Omin 00s to 9h 99min and Hold External dimensions HxWxD C20 model 11 x 16 1 2 x 20 1 2 280 x 420 x 520 mm C 20 R model 11 x 16 1 2 x 28 280 x 420 x 710 mm 230V 10 50 Hz 120 V 10 60 Hz C 20 R Power supply Model Version 230 V 120 V 230 V 120 V Rega mu Model Description Catalog Numbers C20 Version 230V 50 60Hz AW C 020 CVE C 20 R Version 230V 50Hz AW C 020 CRE C 20 R Version 220V 60Hz AW C 020 CRF C20 Version 120V 60Hz AW C 020 CV C
26. owe international AWEL CENTRIFUGES User Manual C 12 C 20 20 R amp C 48 48 R Models OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com ss awel international First edition Written by Checked by 10 Feb 2011 S GERARD L TANNEAU G PICKETT Updates EREN 15 Sept 2011 S GERARD G PICKETT OM0204 Rev 1 March 2012 Best Products Best Performance Best Protection NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com CONTENTS 1 WARRANTY mmm nennen nnn enne na nennen nane na nnn n D 2 GENERAL niacina E EE 5 2 1 Environment See Section 4 3 1 Environment 5 2 2 Technical specifications mmm nennen 5 2 3 Dimensions Positions of the feet 8 AA GG ELLE Ny LOIDIO anime 9 75 PADOS saio E EE m 10 Se LCA ALL ESP Niort ri E 11 ZG COMO LR aa EEE Ann ds DAS ia dr sia Canas sea 11 2 6 1 Practical notes on centrifugation mmn 11 2 6 2 Actions forbidden during centrifugation me 11 3i DESCRIPTION OF THE CENTRIFUGEN mmn 12 Side 356neFdl VIBU ia a RIEN 12 3 2 CONTOS ANO DISDIAV cusspieszeceis diss pure PEE Ea ber Dens en dd um bcr vr sean san dona Ya dd 12 58 9 Sal ety deVIC B5 raiar 12 3 3 1 Device for closing and locking
27. ram created can be locked in order to protect it against accidental modification Press the Lid key ic for 3 seconds The display becomes red and displays protected to indicate that the program has been locked To unlock a program recall the program Press the Lid key ic for 3 seconds The display becomes green and displays unprotected to indicate that the program has been unlocked OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 22 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 6 2 3 Program selection Recall the program by pressing the Q key short press The program numbers will appear sequentially when turning the knob Stop turning the knob once the required number is displayed The word prog stops flashing after 5 seconds The centrifuge is ready to start the selected program Note The numbers of locked programs appear in red 6 2 4 Modification of a program Recall the program 8 6 2 3 Modify the value s required Press the Q key for a long time The display becomes green for several seconds to confirm that the values have been saved 6 3 Using a program After having defined the parameters fitted and correctly loaded the rotor then closed the lid press the key to launch the selected program or a routine configuration OMO204 Rev 1 March 2012 Best Products Bes
28. ry radiation It is forbidden to use rotors and accessories having exceeded the maximum usable lifetime of 10 years or 35 000 runs 1 in normal use condition amp maintenance or showing signs of wear traces of corrosion or mechanical faults 1 On 1st of 2 terms expired OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 11 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 3 DESCRI PTI ON OF THE CENTRI FUGE 3 1 General view Ventilated model Refrigerated model 3 2 Controls and Display Fr kn c sas hy S C u8 m ewel centrifugation Refrigerated Models 3 3 Safety devices 3 3 1 Device for closing and locking the lid C series centrifuges are fitted with a device for locking the lid to ensure the safety of the operator The centrifugation run can only start once the lid is correctly shut and locked During the run it is impossible to open the lid This is only unlocked once the rotating accessories have come to a complete stop OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 12 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 3 3 2 Manual unlocking of the lid Only in case of electrical problem Manual unlocking of the lid must
29. socket with a ground an earth connection that corresponds to the indications on the manufacturer s plate on the centrifuge o Install the centrifuge in a well ventilated space on a rigid horizontal support that can absorb vibration generated by the centrifuge Do not lean over the centrifuge during a centrifugation run Do not remain within the clearance envelope 30 cm around the centrifuge longer than necessary for service reasons Do not place potentially dangerous material within the operating space Use accessories sealed against aerosols when centrifuging and material that presents a biological risk Check the mounting and balancing of the rotor before starting this centrifugation run Take care to maintain and check the accessories See section 7 Keep the centrifugation chamber clean and dry Check that bottles and tubes can withstand centrifugation chemical resistance to the products centrifuged and mechanical resistance to the centrifugal force applied to them o When moving the centrifuge from a cold environment to a warmer environment condensation can form inside the centrifuge Leave the centrifuge to warm up for three hours before use o If the centrifuge is used in a manner not specified in the present manual the protection provided by the equipment may be impaired O O O O O 2 6 Correct use Laboratory centrifuges are used to separate substances of different densities by the application of centrifugal
30. st the support or bench if necessary OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 15 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 4 4 Use e wel centrifugatiaoan 7 EN t 3 A LA hk ho Y v CY8 R Refrigerated models 4 4 1 Switching on the centrifuge The switch circuit breaker for switching on the centrifuge is found on the front left hand side of the instrument 4 4 2 Lid opening The centrifuge that you have purchased is equipped with a motorized lid lock Manually lift the lid to open completely For the first start the centrifuge is delivered with its lid opened It may be that after a prolonged storage of the machine the gas springs have a longer response time The first opening may be less straightforward In this case do some manual movements with the lid in order to properly activate the opening mechanism If the lid is closed press the lid button D and support the lid with the left hand if necessary 4 4 3 Installing a rotor 4 4 3 1 Rotor mounting Position the rotor over the drive shaft Depending on the centrifuge model rotors are distinguished with the locking nut C 12 amp C 48 48 R models Nut with grooves C 20 20 R models Nut without grooves OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 16 of 35
31. t Performance Best Protection Page 23 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 7 CARE AND MAI NTENANCE 7 1 Daily care and cleaning 7 1 1 External body Wipe off marks from the paintwork of the external body and keyboard using an absorbent paper and lightly wetted with an alcohol water solution or a quaternary ammonium compound 7 1 2 Bowl and accessories Keep the centrifugation bowl and accessories clean and dry NEVER USE CHLORINATED PRODUCTS bleach quaternary ammonium chloride 7 2 Weekly care and cleaning Wash the rotors with clean or distilled water Dry them carefully particularly the parts that are not easy to access When necessary use a hairdryer to completely dry the small diameter pockets in the rotor Do not forget to clean the central part of the rotor with a soft non metallic brush After cleaning add a light film of silicone using a spray or lanolin to the surface of the rotor and in the pockets This precaution will extend the life of metallic accessories Swing out rotors Check the greasing of the pins on which the buckets swing Replace this regularly at least once per month in order to ensure that the buckets swing freely Spread on a little grease by hand WARNI NG Failure to grease correctly could create an imbalance related to one or more buckets not swinging Use Food quality grease on
32. the speed centrifugation radius and relative centrifugal force to be displayed successively Select the required value with the help of the knob while in programming mode The new value is automatically saved after 5 seconds or if the key for another parameter is pressed Do not forget to set the radius value RAD of the mounted rotor to obtain the speed RPM or the centrifuge acceleration RCF required Refer to the accessories table to determine the maximum speed If the set speed is too high corresponding to the installed rotor the spinning speed is automatically limited In this case the spinning flash turns differently and 3 beep sounds are regularly emitted The speed setting is adjusted to the maximum allowed for the rotor installed OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 21 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 6 1 2 Time 6 1 2 1 Programming the Time la Press the Time key firmly until the display of the time goes to programming mode only the time value appears in a violet color Select the required time value with the help of the knob while in programming mode Selectable range From OminOs in steps of 10 seconds then from 1h00min to 99h59min in steps of 1 minute The new value is automatically saved after 5 seconds or if the key for another parameter is pressed 6 1 2 2
33. uAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com Angular rotor dynamic balancing The centrifuge can tolerate an imbalance of 10 g Important Note The imbalance detection system automatically shuts down the centrifuge in case of excessive imbalance gt 25 g and displays the error code Err01 Moderate imbalance 10 to 25 g generates vibrations that can cause or stop by detecting imbalance Err01 or a cap at low speed An excessive imbalance is likely to seriously damage the rotor and the centrifuge 4 4 5 Closing the lid Once the rotor has been loaded to close the lid put your hand on the blue handle and lower the lid Keep pressing the lid during the blinking of the handle A buzzer sounds for the interlock You can release the lid which is now locked Warning Place the hand flat on the top of the lid while pushing it OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 18 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 4 4 6 Detection of locking failure The locking device is designed to be closed in a determined time If not detected in 2 seconds the locking device motor disengages to allow the obstacle to be released The display indicates Press Lid Button Once cleared press the key to reinitiali
34. ve bag Autoclave the two bags for 15 minutes at 122 5 C 252 5 F or according to the appropriate protocol for the material centrifuged Deal with the bag containing the broken tubes according to the method for treating cutting slicing contaminated waste Remove the cleaning residues from the accessory according to the protocol for eliminating biological waste from the laboratory 7 4 3 Material presenting a radioactive risk Comply with the decontamination protocol in use in the laboratory OMO204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 24 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 7 4 4 Material presenting a toxic risk Comply with the decontamination protocol in use in the laboratory 7 5 Sterilization and disinfection of the centrifuge and its accessories Comply with the decontamination protocol in use in the laboratory The following solutions are recommended 70 Ethanol in water solution Non halogenated quaternary ammonium solutions For autoclavable accessories autoclave at 121 C 252 F for 15 minutes Refer to the accessories documentation for the autoclaving possibility When autoclaved lids seals buckets and rotors should be separated from each other The use of other products is subject to the prior agreement of AWEL International Before using any cleaning or decontaminating metho
35. w nuaire com 4 3 2 Power supply Check that the electrical supply corresponds to the indications on the manufacturer s plate Power supply to the instrument must be provided by a socket with a ground an earth connection and a protective device ensuring that the power is automatically cut in case of a fault in the insulation A supply fitted with a differential circuit breaker of a suitable rating satisfies this requirement To allow electrical isolation of the device the socket must be readily identifiable and within easy reach of the operator The electrical installation must include an emergency stop switch enabling the supply to be cut in the case of irregular operation This switch must be placed at a distance from the centrifuge outside the limits of the centrifuge operating space and preferably outside of the room in which the centrifuge is placed 4 3 3 Place of installation Place the centrifuge on its support or on a flat horizontal bench that is fixed and can support its weight see section 2 2 Technical specifications Take care to mark out an operating space 30 cm all around the instrument Do not block the ventilation slots on the sides of the instrument towards the front Leave at least 35 mm space behind the centrifuge and 50 mm on each side Mount a rotor in the centrifuge see section 4 4 3 Installing a rotor Place a spirit level on the rotor and check the flatness in two directions at 90 to each other Adju
36. ze the lock If the obstacle is more than 6 mm the lock is not activated pinch hazard is eliminated by detection of locking failure device If closed on the fingers pressing occurs then relaxes opening the lid 4 4 7 Automatic speed limitation If the set speed is too high corresponding to the installed rotor the spinning speed is automatically limited In this case the spinning flash turns differently and 3 beep sounds are regularly emitted The speed setting is adjusted to the maximum allowed for the rotor installed 4 4 8 Detection of end of run AWELight To visually identify the end of a cycle the light in the handle flashes The and keys also flash indicating the possibility to open the lid or to start a new cycle End of run indication AWELight Device 0M0204 Rev 1 March 2012 Best Products Best Performance Best Protection Page 19 of 35 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 5 CONTROL PANEL Display D Program Number Speed Radius RCF Relative Centrifugal Force 3 Time a Temperature Refrigerated models centrifugation C48 R 0 Sssowel A s it Control keys 5 Adjustment knob ntuitive keys 6 Stop and Escape For an easier use of the control keys 7 Start keys O and O brighten up when 8 Open Lid Lock Program the
Download Pdf Manuals
Related Search