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1. 2 121 30 3 10 2 10 12 11 10 12 1 Members of the American College of Chest Physicians Society of Critical Care Medicine Consensus Conference Committee Crit Care Med 20 864 1992 2 Medical Technology 27 1372 1999 3 181 8 1997 4 181 3 1997 5 181 96 1997 6
2. 6 g8 1 4 DMSO PMSF 5mol LL 5mol LL 0 140G 180G 37 42 4 5 6 42 7 8 5 L 1 0004L 10mL 50mL 150mL 1 mL 0 2zm 50mL 1 1 PBS 1 6 mL 114mL
3. 37 30 2 4 0 5 GO 1 1 G3 3 4 M NNN SNSN SN EEE NN kN NNNNN NN RR SR SH dr EL CE 1 4 7 X1 000 1 1 292 ISAl
4. gt 3 4 140G 180G 10 1 mL P B S 0 4 140G 180G 10 2 1 PBS 4 000 6 500 4L 5m L PBS200 L 2 1X10 5 X10 4L PBS 5wL APS 20 759 5 0 750
5. DNA 8 1 1 2 3 4 2 10 25mL 10 50mL 1 X10 2 10 10
6. 1 241 1999 7 5 225 1994 8 72 211 1995 9 41 1347 1999 10 BIO Clinica 14 97 1999 1 1 Shimada J et al J Infect Chemother 5 21 1999 536 8523 3 30 TEL 06 6964 2763 FAX 06 6964 2706 9 00 17 30 3 11 UM 115 115E
7. 6 DN A 2 DNA 1 1 70mmol PPBS 3 2 75 85 989 3 1 4 SS Rh A 2 J tv ACN WY 1 75 85 98 70mmol PBS 7 2 DNA 1 1 Ce SA 8 1 7 5 L 2 2 37 42 2 3 3 42C 10 3 4 PBS 10
8. 2 30mL 15mL 5mL 3 5 mL 45mL 4 1 1 mL 1 PMSF 1mg DMSO 2 50 PBS 5 mL 5 0 2mol L PBS 1 5 mol L 2 5mL 2mL 45 5mL 6 5mL 44 6mL 0 4mL 7 70mmol L PBS 1 5 mol L 2 5 mL 0 7mL 46 8mL 8 50mLx 3
9. situ DNA 1 2 EE 3 4 DNA DNA 2 DNA 1 DNA ED LD a DNA I 5 DNA 6 Hybrid 7 1 000 5
10. 2 7 5mL 75mL 67 5mL 42 C 9 2 L 98uL 1 0 1 2 5 mL 5mL 40mL 1 3 50mLx 2 3 10mL 90mL SM 2 4 5 mL 45mL 3 1 0 5mL 49 5mL ee 1 224 I y 504L 1 5mL 10mL 2 4 1 37 C 30
11. 3 1 P B S 10 2 10 SN 1 A Ss ww Pe 4 DN A 1 1 1 mL 37 42 30 2 0 2mol P BS 10 0 2mol L PBS 5 1 10 2 75 85 98 3 a RE TRI
12. SS SS NN NN NN PBS 10 3 9 1 1 mL 30 10 1 104L 30 C 2 3 2 10 2 3 10 NX NN S amp S NN X NN NSJ A 3 2 11 1 1 1 mL 0 2zm
13. 50mL SAI S ag7egs DNA SE S epidermidis DNA P Al aeruginosa DNA EF eca7s DNA EK E co7 E cloacae K pneumoniae DNA DNA DNA 50zL 2 10C 10mL 50 1 2 10 mL 2 10 1 10 50mL 2 10 50mL TEF EK NBT 3 BCTP 10 FCF
14. 1 2 DNase 3
15. 3 1 20 120mL 2 20 75mL 3 10 100mL 4 10 50mL 8 AFS 3 S A 10mL 50mL Ni Staphylococcus aureus Staphylococcus epidermidis Pseudomonas aeruginosa Enterococcus faecalis Escherichia coli Enterobacter cloacae Klebsiella pneumoniae SE P A EF EK 4
16. 2009 10 1 2 2009 7 1 67749 121100AMZ00620000 HYBRISEP SAl SE P AI SIRS systemic inflammatory response syndrome sepsis SIRS SIRS 49 SIRS OO
17. HBV HCV 3 gt 1 2 3 4 1 0 08 6
18. ISE PAl IEF EK 123 42 32 11 4 2 2 3 3 9 1 2 HIV
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