DNAプローブ「FR」- MTD®
Contents
1. RNA rRNA RNA AACO03T 2006 10 2 2006 4 1 a RR 1 TMA Transcription Mediated Amplification 1 2 21500AMY00012000 R NA RNA DNA RNA T 7 RNA RT DNA F R MTD DNA cDNA RT RNase HH y gt iiW74 ZN AS RNA2. 6 4 1 1 4 1 AB 3 mL 2
3. O 2 0 0 9 5 5 1 5 C9 95C 2 5 1 0 0 z S 300 i 1 0 Relative Light Unit 20 gL 200 gL 15 25 x Ds 50TMA Ds 50HPA 4
4. 1 2 8 2 3 4 3 1 2 3 2 5 lt 0 E 2 OS
5. 4 2 1 5 Es NS 6 0 1 1 5 6 0 1 1 5 RLU TMA HPA 3 0 0 0 0RLU 3 0 0 0 0RLU 2 1 2 1 0 8 9 0
6. 2 3 tuberculosis M 2or7s bovis BCG africanum micerotz 4 1 2 3 1 2 2 8
7. NTMIRNIINIIINIIAIIINIINIINITANIIANTINIIALI A RNa seH 4 DNA 5 6 DNA GU DML T7 mmmmmmmmmmmm ImmmmmTTmmm DONA 7 T7 Ka ra EE g i a yA ENA PT NII DNA
8. 4 E MMLV 250U 2 T7 RNA 16 7U RNA AE RNA DNA RNA DNA M gt 5 0 1 3 0 0 6 es CR 1 i COVTROL 8 Cc agyt 7 CONTROL A A G DNA
9. 5 1 2 SN a 2 5 2 5 5
10. LT 2 0 L 2 0 0 3 9 5 5 2 0 9 5 5 5 9 5 5C 5 9 5 5C Ds 50TMA Ds 5 0HPA 3 TMA
11. 2 5 NE 1 5 2 5 MD 4 1 2 0 5 0 3 0 1 C Abe et al Detection of Mycecobacterium tuberculosis in Clinical Specimens by Polymerase Chain Reaction and Gen Probe Amplified Mycobacterium Tuberculosis Direct Test J Clin Microbio1 31 3270 3274 1993 2
12. I 5 0 5 0 i 2 RLU Relative Light Unit em ll 20 kL 200 x 25 ux 50zL 200 gL 95C 15 42C 5 SN 00 60C 15 10 3 2 1 4 7 3 1 1 2
13. T 5 0 5 0 i 2 RLU Relative Light Unit OO 5 TMA Transcription Mediated Amplifcation HP A Hybridization Protection Assay 0 S I 0 CONTROL CONTROL 2 5 gg ON CONTROL CONTROL
14. 500 5 DR 00RLU FE 4 3 1 0 Loewenstein Jensen H 4 8 9 8 Gen Probe RNA rRNA DNA
15. 2 62 5 TEL 03 5695 9210 FAX 03 5695 9234 BY 2 62 5 Gen Probe Incorporated
16. 2 HP A 1 1 2 3 NALC NaO NALC Na OH p 2 1
17. I oO Oo Il i2mm 95Cxi O Ci oC Oo 20gL 25gL 50pL 100 gL 200gL 300pL Ds 50HPA Ds 5 0TMA Ds 50HPA 3 1 1 3 7 1mL 1 5 mL NALC Na OH 0 2 0
18. 100 1000 8 RNA NL RT NO u DNA RNaseH BNA IIINIIINIIINIIIDIINUIINIINIIDIINTINIINNIINI RT MMLV y RNA pol T7 RNA H 1 TMA 1 5 jf DNA 1 2ng HB 2 HPA Hybridization Protection Assay 3 R NA DNA RNA RNA DNA
19. 2 0 15 koo J OB 2 3 TMA Transcription Mediated Amplifcation 9 5 15 12x75mm 5 0 0 2 0 0 CONTROL CONTROL 2 5 9 5 5C 1 5
20. E 1 EDB 1 5mL 3 I 1 HB 6 mL 4 2 _ oO oO 2 NaOH 1 45 Na citrate 0 5 N acetyl L cysteine looezmuZrkupmesl oO 0 15mL O O NALC Na OH 1510J 42kHz 90W 12X7 5mm MTD skzoorimr O O sa on im Oo O CE CY RE
21. 2 0 4 2 1 C 5 4 2 2 5 L 4 2 1 3 0 1 2 2 4 4 3 3 0 2 8C 2 2 0 C 1 2 8 4 2 0 60 1 4 4
22. 0 HP A Hybridization Protection Assay 3 1 0 0 7 w 9 mia 1 6 0 1 1 5 20 S ts i 8 3 0 0 6 0 1 1 5 1 6 1 0
23. cDNA R DNA DNA RNA RNA Po1 RNA 1 RNA DNA RNA 1 1 T7 ii RRNA 2 RY 3 a
24. 1 2 8 4 2 3 2 8 1 8 4 6 0 1 2 8 3 2 8 6 0 5 1I 50 5 0 i
25. 1 5 Th EL 1 1 0 979 0 874 0 895 HTIV HBV HCV 0046 2 E 3
26. GRNA Gen Probe MTD MB 69 7 14 1994 3 HPA ACCUPROBE 50 310 314 1992 4 MTD 2 69 945 949 995 5 M 0sumi et al Detection of Mycobacterium tuberculosis in Clinical Specimens Other than Sputum by a Specific DNA Probe with Amplifcation of the Ribosomal RNA 69 1376 1382 1995 6 S Ehlers et al Diagnosis of Extrapulmonary Tuberculosis by Gen Probe Amplified Mycobacterium tuberculosis Direct Test J Clin Microbio1 34 2275 2279 1996 7 MTD 71 495 503 1996 8 2000 2001 9 1990 66 323 332 1991
27. 1 3 0 2 8 2 2 0 1 2 8 C 4 2 0 C 6 0 1 C 4 4 HP A Hybridization Protection Assay Ds 50HPA NaN i Ds 50HPA S 1 0 0 gm L 6 0 1 1 5 3 0 0 6 0 1C 1 5 CR E 0
28. 1 0 0 0 6 7mo1 pH6 8 1 0mL _ 3 000Xg 1 5 0 067mo 1 pH6 8 1mL p p HH 4 to 2 LT SDB 0 7 2 0 0 8 1 5
29. Transcription Mediated Amplifcation Ds 50TMA Ds 50TMA 0 12 75mm CONTROL CONTROL 2 5 gm L Ds 5 0TMA CONTROL CONTROL 1 2 OC 9 5 5 1 5 4 2 1 5 2 5 4 2
30. 2 7 1 1 1 9 000RLU 2 1 0 0 150 000RLU 3 4 H GC 3 CV FU assay 2 1 2 3 20 000 6 DNA
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