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Operating Manual and User's Guide

1. Specifications Spectral range 200 400 nm Time to stabilized output 30 minutes Power consumption 25 30 Watts Bulb lifetime 1 000 hours replacement bulbs available Stability peak to peak 0 05 maximum drift of 0 5 hour Aperture 0 5 mm at lamp Connector SMA 905 Window material 0 4 mm thickness UV transmissive glass Lamp voltage 85 volts DC nominal Operating lamp current 300 mA DC 1mA Timing microprocessor based Inputs trigger inputs for lamp on off Outputs levels for lamp on off filament on off 120 volts AC 0 50 A 50 60 Hz Powenrequiremants 220 volts AC 0 25 A 50 60 Hz 100 volts AC 0 60 A 50 60 Hz 240 volts AC 0 20 A 50 60 Hz 37 F Light Sources DT 1000 phis NO DT 1000 Deuterium Tungsten Halogen Light Source The DT 1000 DEUTERIUM TUNGSTEN HALOGEN LIGHT SOURCE combines the continuous spectrum of a deuterium UV light source and a tungsten halogen VIS Shortwave NIR light source in a single optical path The combined light source produces a powerful stable output from 200 1100 nm It also has a highly stabilized microprocessor based power supply designed for optimum stability Parts Included DT 1000 Deuterium Tungsten Halogen Light Source Safety eyewear for protection against dangerous ultraviolet radiation Power cord for connecting DT 1000 to outlet Allen wrench for adjusting the focus of the collimating lens eee d
2. Connector SMA 905 Power input 12 V Power requirement 12 VDC 420 mA Power consumption 5 watts deuterium 3 8 watts tungsten 1 2 watts 67 F Sampling Chambers FHS UV FHS VIS O FHS UV and FHS VIS In line Filter Holders The FHS UV and FHS VIS IN LINE FILTER HOLDERS are low cost spectrophotometric accessories for fast convenient absorbance transmission measurements of optical and other filters Optimized for VIS Shortwave NIR 360 1000 nm or UV VIS Shortwave NIR 200 1100 nm measurements each holder has a pair of 5 mm diameter f 2 collimating lenses to maximize light throughput and comes with a manual light block wheel for taking dark readings The FHS In line Filter Holder accepts 1 round or any size gt 1 2 square optical filters up to 14 6 mm in thickness Plastic supports hold SMA terminated fibers steady during use The light that passes through the filter is collimated This process makes it possible to measure either glass or interference filters Operation Installing Filters 1 Loosen the filter thumb wheel on the left side of the filter holder to create enough space for the filter you wish to use If the filter is round place it in the filter slot If the filter is square place it in the filter slot at a 45 angle so the filter is in the beam path Clamp the filter in place by tightening the thumb wheel When filters are not being used in the holder tighten the thumb wheel c
3. 73 ean iptics Inc First in Photonics Sampling Optics Ocean Optics offers numerous spectroscopic accessories A short description for each accessory featured in this manual is listed below The 74 UV and 74 VIS COLLIMATING LENSES screw onto the end of SMA terminated optical fibers and other sampling optics to convert divergent beams of radiation light into a parallel beam The 74 90 UV RIGHT ANGLE REFLECTOR for collimating lenses has a mirror located under its cap that reflects light from the collimating lens to 90 The 74 90 UV is a 3 8 24 threaded black anodized aluminum assembly for mounting our collimating lenses at right angles and is useful for applications that involve limited space and inconvenient optical fiber routing The 74 OPM OPTICAL Post MOUNT is a 3 8 24 threaded black anodized aluminum assembly used for mounting collimating lenses on breadboard laboratory tables rail carriers and other bench plates The 74 ACH ADJUSTABLE COLLIMATING LENS HOLDER is a versatile assembly for mounting lenses at multiple positions and is especially useful for large or thick samples not easily accommodated by other sampling optics such as our FHS UV and FHS VIS In line Filter Holders The FVA UV FIBER OPTIC VARIABLE ATTENUATOR is an opto mechanical device that controls the amount of light being transmitted by a fiber and the amount of light entering the optical bench of the spectrometer The attenuator can
4. Specifications Path length 1cm Collimating lenses VIS NIR 2 BK 7 glass 360 nm 2 um 5 mm diameter f 2 Collimating lenses UV VIS NIR 2 Dynasil 1100 quartz 200 nm 2 um 5 mm diameter f 2 Collimating lens termination SMA 905 Fluorescence option position 2 collimators at 90 Filter slot accepts filters up to 1 4 6 mm in thickness Base material aluminum Water input fittings 1 8 NPT pipe thread Though the VIS NIR lens is optimized for use to 2 um it can be configured to see only to 1100 nm with our S2000 spectrometer 59 F Sampling Chambers CUV FL DA O CUV FL DA Direct Attach Cuvette Holder The CUV FL DA DIRECT ATTACH CUVETTE HOLDER attaches directly to our light sources and couples via SMA terminated optical fibers to our spectrometers creating an incredibly small footprint spectrophotometric system for fluorescence as well as absorbance experiments The CUV FL DA optimized for UV VIS NIR 200 1100 nm applications holds 1 cm square cuvettes The CUV FL DA is especially useful for fluorescence measurements Mirrored screw plugs have been incorporated into the CUV FL DA to make the fluorescence signal stronger These mirrors are UV enhanced aluminum coated for optimal signal reflection One mirrored screw plug collects the fluorescence that would otherwise be lost The second screw plug works to reflect the excitation energy back through the sample The increase in signal intensi
5. Time to stabilized output 30 minutes Bulb output 7400 foot candles 7 4MSCP Though the product can be used to 2 um it can be configured to see only to 1100 nm with our S2000 spectrometer 65 Sampling Chambers ISS UV VIS CO ISS UV VIS Integrated Sampling System The ISS UV VIS INTEGRATED SAMPLING SYSTEM is a combination of a RF deuterium source with a tungsten bulb connected to a cuvette holder for 1 cm cuvettes The cuvette holder attaches directly to the light source and has a 5 mm diameter f 2 collimating lens The ISS UV VIS can be operated manually or through the software Parts Included ISS UV VIS Integrated Sampling System Power cord for connecting the ISS UV VIS to outlet 15 pin accessory cable for software control of the ISS UV VIS Caution kA The beam emerging from the ISS UV VIS produces ultraviolet radiation Direct eye contact could cause eye injury Safety eyewear is recommended Never look directly into the light source or stare at the diffuse reflected beam Dangerous voltages present NO serviceable parts inside unit The deuterium and tungsten bulbs cannot be replaced by unauthorized personal To replace a bulb in the ISS UV VIS contact Ocean Optics For any experiments using UV light we recommend using our solarization resistant fiber SBE Tye This instrument should not be used for any clinical or diagnostic purposes Operation Adjusting the Fit of the Cuve
6. Specifications Diffusing material Opaline glass or Teflon Barrel dimension 0 25 OD Sampling geometry accepts light at from 180 FOV Connector SMA 905 87 ean Fiber Optic Probes and Accessories FL 400 CO a FL 400 Flame resistant Fiber Probe The FL 400 FLAME RESISTANT FIBER PROBE is a heat resistant fiber optic probe that couples to Ocean Optics miniature fiber optic spectrometers to measure in situ emission spectra of samples such as dissolved metals and high temperature plasmas The FL 400 is a high temperature 400 um gold jacketed UV VIS optical fiber in an 8 long nickel sleeve It can operate in environments up to 750 C The probe comes with a standard wire loop for emission measurements of dissolved metals Not included though necessary for operation is an optical fiber and a splice bushing for connecting the FL 400 to the optical fiber Operation 1 Twist the male end of the FL 400 into a 21 02 Splice Bushing 2 Connect a standard optical fiber normally a P400 2 UV VIS 400 um optical fiber to the other end of the splice bushing 3 To observe flame emission spectra of samples such as sodium potassium calcium and copper attach the wire loop to the FL 400 by slipping the FL400 into the coil spring of the wire loop Specifications Fiber core diameter 400 um Fiber core cladding Silica Fiber core cladding diameter 440 um Fiber jacketing Gold Fiber core clad
7. If you do not see the Configure Hardware screen exit the software Then select Start Run and type C windows ooidrv ini for Windows 95 98 systems or c winnt ooidrv ini for Windows NT systems Notepad will open Edit this file for our device driver by finding the Initialized entry and making sure this line reads Initialized 0 Save the OOIDRV INI file and exit Notepad Restart OO Base32 You should now see the Configure Hardware dialog box Spectrometer Configuration Dialog Box Spectrometer Configuration Ei Now that OOIBase32 is running you need to Wavelength Calibration A D Interface Reference Monitoring Stray Light Correction Detector Linearity configure your system Select Spectrometer Spectrometer Type 52000 PC2000 gt Configure from the menu Go through each A D Converter Type saps00 page in the Spectrometer Configuration dialog box to set system parameters See the OOIBase32 Spectrometer Operating Software Manual for details In the Wavelength Calibration page the SAD500 Serial Pot coms v SADSOO Pinel Resoluti coefficients for each spectrometer channel mmm ME z EA have already been loaded as part of the SADSOO Baud Rate 38400 z F Compress SAD500 Data spectrometer configuration file Check the Enabled box for each spectrometer channel in your system In the A D Interface page enter the same settings and values as you did in the Configure Hardware dialog box The Detector Lin
8. Spectral range calibrated 300 1050 nm Dimensions 9 0 cm x 5 0 cm x 3 2 cm LWH 3 5 x 2 0 x 1 25 LWH Power input 12 VDC 800 mA regulated Power output 6 5 watts Bulb life 900 hours recalibrate after 50 hours of use Bulb color temperature 3100K Output to bulb 5 volts 1 3 amps Output regulation 0 2 voltage Time to stabilized output 15 minutes Connector SMA 905 53 ean IPLICS inc First in Photonics Sampling Chambers Ocean Optics offers a comprehensive line of compact low cost sampling chambers for a variety of UV VIS Shortwave NIR applications All sampling chambers have SMA terminations for easy coupling to optical fibers Options include a variety of cuvette holders for 1 cm and 10 cm cuvettes in line filter holders flow cells and other sampling devices CUV UV and CUV VIS CUVETTE HOLDERS are our standard cuvette holders for 1 cm cuvettes The CUV UV 10 and CUV VIS 10 CUVETTE HOLDERS are cuvette holders for 10 cm paths They all come with a pair of 5 mm diameter f 2 collimating lenses and filter slots The CUV ALL 4 way CUVETTE HOLDER is a cuvette holder for 1 cm cuvettes that has fiber optic couplings at four collimators a pair of collimating lenses optimized for the UV VIS and a pair optimized for the VIS NIR Also available is the CUV FL DA DIRECT ATTACH CUVETTE HOLDER a cm cuvette holder that attaches directly to ligh
9. When prompted to do so restart your computer when the installation is complete NNW Step 3 Configure OOIBase32 Software After you restart your computer navigate to the OOIBase32 icon and select it Now that your A D converter and software have been installed you need to configure your software For details on using OOIBase32 refer to the OOIBase32 Spectrometer Operating Software Manual Operator and Serial Number Dialog Box First a prompt to enter a user name and serial number appears Some files will include this data in the header Default Spectrometer Configuration File Next a message appears asking you to select a default spectrometer configuration file A file open dialog box then appears You must choose the default spectrometer configuration file Navigate to the OOIBase32 directory and choose the file with spec as the extension The spec extension is preceded by the serial number of your spectrometer I2J613 spec is an example of a spectrometer configuration filename Configure Hardware Dialog Box Next the Configure Hardware dialog box opens The parameters in this dialog box are usually set only once when OOIBase32 is first installed and the software first opens Choose the spectrometer and A D converter you are using For the Base Address and IRQ choose available settings If you have the SAD500 specify the same COM port number as the one being used to interface to your SAD500 Spectrometer Configuration Dial
10. a g Windows NT Diagnostics DAYENT Oix g Windows NT Diagnostics DAYENT File Help File Help Version System Display Drives Memory Version System Display Drives Memory Services Resources Environment Network Services Resources Environment Network Include HAL resources I Include HAL resources Rg Device Bus Type Address Device Bus Tye 01 i8042prt 0 Isa 0060 0060 i8D42prt 0 sa 03 Serial 0 Isa 0064 0064 i8042prt 0 Isa 04 Serial 0 Isa 0170 0177 atapi 0 sa 06 Floppy 0 Isa OICE O1CF VgaSave 0 Pci 10 El90x 0 Pci 01F0 01F7 atapi 0 Isa 12 i8042prt 0 Isa 02F8 02FE Serial 0 sa 14 atapi 0 Isa 0376 0376 atapi 0 sa 15 atapi 0 Isa 0378 0374 Parport 0 Isa 0380 03BB8 VgaSave 0 Pci 03C0 03CF STBinc 0 Pci 03C0 03DF VgaSave 0 Pci 03D4 03DB STBInc 0 Pci O3F0 03F5 Floppy 0 sa O3F6 O3F6 atapi 0 Isa 03F7 03F7 Flooov 0 ta Z IRQ 120 Port DMA Memory Devices IRQ DMA Memory Devices Properties Refresh Print Properties Refresh Print F ean A D Converters DAQ 700 P er Run OOIBase32 After you restart your computer navigate to the OOIBase32 icon and select it The very first time you run OOlIBase32 after installation you must follow several prompts before you can begin taking measurements Operator and Serial Number Dialog Box First a prompt to enter a user name and serial number appears Certain data files will include this information in the header If at a later da
11. E for excludes position means that the resulting reflection measurement excludes specular reflection the user will only obtain diffuse reflection measurements Move the switch to the mode necessary for your application Using the Optical Fiber Ports The ISP REF has SMA connectors for two optical fibers The connector or port marked S for sample is used to couple an optical fiber to the spectrometer to measure the reflection from a flat surface The second port marked R for reference offers two features not available with most other integrating spheres One function of the R port is to couple an optical fiber to a second channel in the spectrometer This channel can be used to monitor the Integrating Sphere s built in tungsten halogen lamp which provides even surface illumination The other function of the R port is for the coupling of an optical fiber to collect light This may be advantageous for applications involving the collection of a wide angle beam of light especially where the beam is much larger than the size of the entrance optics Specifications Spectral range of illumination source 360 1000 nm Dimensions 2 11 x 2 25 x 3 25 LWH Sphere diameter 1 5 Sample port aperture 0 4 Sphere material Spectralon Reflectance measurements specular included or excluded Bulb life 900 hours Bulb color temperature 3100K 81 First in Photonics Sampling Optics
12. Connect the other fiber to the spectrometer we recommend a unit with an L2 lens and either grating 1 or 2 a 25 um slit the UV2 detector upgrade and an OFLV 200 850 order sorting filter Sample tube 72 F ean Sampling Chambers CUV CCE Orr Checking the Alignment 1 With the spectrometer running observe the signal in Scope Mode When the optical window is properly positioned you can see a full UV transmission through 37 the cell If the polyimide is in the optical path you lt will see just the red end of the spectra If this occurs loosen the fittings and slide the sample tube to align with the window until you achieve the best signal on both the wavelength and intensity axes 2 Ifthe fibers are not properly inserted in the through I hole the intensity will be low To maximize intensity loosen the fiber fittings and adjust the fiber 3 When the fibers and sample tubing are perfectly aligned make sure all fittings are snug P e 729 Peektm Cross 4 Mount the cell in your apparatus using the mounting holes It is important that the optical fibers are not moved during measurements If necessary secure the optical fibers to relieve stress especially where the fibers connect to the cell Mounting Holes Performing CUV CCE Measurements in OOIBase32 1 Make sure you are in Scope Mode Select boxcar smoothing and signal averaging values and an integration period that won t saturate the detector 2 W
13. FOIS 1 CO FOIS 1 Fiber Optic Integrating Sphere The FOIS 1 FIBER OPTIC INTEGRATING SPHERE is a sampling optic that accepts light energy through its 0 375 input port and funnels it to an optical fiber for emission experiments such as measuring the spectral properties of LEDs and other light sources The FOIS 1 consists of a 1 5 Spectralon sphere encased in an aluminum housing with a 0 375 input port that accepts light energy from 200 1100 nm and a SMA connector for coupling to the spectrometer Application Tips The FOIS 1 is small and compact it s just 2 25 x 2 25 x 2 125 and weighs less than 1 pound yet is durable enough for use outside the laboratory The inside of the FOIS 1 is made from Spectralon a white diffusing material that provides a highly lambertian reflecting surface Operation The FOIS 1 is very easy to operate 1 Connect an optical fiber the read fiber from the FOIS 1 s SMA terminated output port to the SMA termination of the spectrometer 2 Insert your emission source into the 0 375 black input port of the FOIS 1 Or configure your setup so that the emission source is aligned so that the light energy can enter the input port 3 To collect radiation light from a 180 field of view thus eliminating light collection interface problems inherent to other sampling devices make sure that you do not insert your emission source too deeply into the FOIS 1 If you insert the emission source into t
14. N C A D Trigger Digital In 3 D3 S1 Ground 5 VDC Phi A D clock Phi Read Out Gate Reserved Temperature optional Read Enable olni a Al olni ib gt rlo oo SO o Strobe Single flash Oo Strobe Multiple Flash e e Integration Clock 4 N Master Clock The continuous strobe rate is controlled by the A D converter frequency input to the spectrometer and the position of a jumper in Jumper Block 3 labeled JP3 on the S2000 circuit board The jumper selects a divider for the input clock Jumper Divisor DAQ700 ADC500 SAD500 ADC1000 Position Flash Rate Flash Rate Flash Rate 1 Buffered input from D25 pin 20 2 510 97 6 Hz 488 0 Hz 976 0 Hz 3 ore 24 4 Hz 122 0 Hz 244 0 Hz 4 ot 6 1 Hz 30 5 Hz 61 0 Hz 5 2 8 1 5 Hz 7 6 Hz 15 2 Hz 107 ean lt tics Appendix E PC2000 Pin outs and Jumpers The average user would not normally need to know about the interconnect scheme of the 2000 as the cables supplied with all of the units need only be plugged into the matching connectors on the hardware However if the need arises to design and fabricate your own cabling system the following tables supply the necessary information H1 Header Block uv 5 Function Channel 1 slave 1 Channel 2 Channel 3 Channel 4 Channel 5 Channel 6 Channel 7 GND GND SO Reserved Multiple Strobe Sing
15. Network Include HAL resources I Include HAL resources I IRQ_ Device Bus Type Address Device Bus Type a i8042prt 0 Isa 0060 0060 i8042prt 0 Isa 03 Serial 0 Isa 0064 0064 i8042prt 0 Isa 04 Serial 0 Isa 0170 0177 atapi 0 Isa 06 Floppy 0 Isa OICE O1CF VgaSave 0 Pci 10 El90x a Pei O1FO O1F atapi 0 Isa 12 i8042prt a Isa 02F8 O2FE Serial 0 Isa 14 atapi a Isa 0376 0376 atapi 0 Isa 15 atapi a Isa 0378 0374 Parport 0 Isa 0380 03BB8 VgaSave 0 Pci 03C0 03CF STBIinc 0 Pci 03C0 03DF VgaSave 0 Pci 03D4 03DB STBInc 0 Pci O3F0 03F5 Floppy 0 Isa O3F6 O3F6 atapi 0 Isa 037 03F7 __ Flooov 0 sa H ro ECER E es Ee n ea a Properties Refresh Pint Properties Refresh Print IL ox With Windows NT devices cannot share IRQs each device must be assigned a unique IRQ Install the ADC1000 or PC2000 Turn off the computer and remove the computer cover Ground yourself to the computer chassis or power supply and remove the A D card from its static shielded bag If necessary change the position of the switches on the A D board Position the switches to match the available settings you found in the previous section numbers not being used by other hardware devices See Appendix A for switch setting positions Find an open ISA bus slot and remove the slot protector Insert the A D card into an available expansion slot on the motherboard by gently rocking the card into the slot Make sure the card
16. The WS 1 is mostly used in a setup with a reflection probe and reflection probe holder for diffuse reflection measurements or with the ISP REF Integrating Sphere To use the WS 1 as a reference in a diffuse reflection measurement 1 Make sure you are in Scope Mode by either clicking the Scope Mode icon on the toolbar or selecting View Scope Mode Take a reference spectrum by first making sure nothing is blocking the light path going to your reference Place the reflection probe over the WS 1 For best results use our Reflection Probe Holder to keep the reflection probe firmly at a 45 angle 2 Take the reference reading by clicking the Store Reference Spectrum icon on the toolbar or selecting File Store Reference Spectrum Specifications Reflectance material Spectralon Reflectivity gt 99 Wavelength range 200 nm 2 5 um Reflective area diameter 1 25 Though the product can be used to 2 5 um it can be configured to see only to 1100 nm with our S2000 spectrometer 80 F Sampling Optics ISP REF Wee ISP REF Integrating Sphere The ISP REF INTEGRATING SPHERE is an illuminated sampling optic that couples via optical fiber to Ocean Optics miniature fiber optic spectrometers to measure reflectance of solid objects or emission of spectral sources The ISP REF Integrating Sphere has a transfer optic assembly for restricting the fiber viewing angle a 0 4 aperture sample port and a built in light so
17. but avoid touching the glass casing or envelope around the bulb as the oils from your skin will deteriorate the bulb Inside the envelope is a triangle shaped filament The filament has a square opening The light passes through the square opening to the collimating lens Take the new bulb carefully feeding the three wires through the hole in the black platform and position it so that the square opening in the filament faces the collimating lens 9 Screw in the two screws that secure the bulb to the black platform 10 Secure the three wires to the green electronic board Attach the red wire to the top screw labeled A Attach the black wires to the screws on the board labeled H and C It does not matter which black wire is attached to screw H or C 11 Put the DT 1000 casing back on and secure it with the 12 screws Replacing the Tungsten Halogen Bulb 1 Order a tungsten halogen replacement bulb item DT 1000 BT from Ocean Optics 2 Remove all 12 screws from the side panels of the DT 1000 casing using a Phillips head screwdriver Do not remove any screws from the front back or bottom panels Remove the cover 3 Locate the tungsten halogen bulb It is inserted on the top of a cylindrical unit held by a set screw in a hole in the black platform The cylindrical unit is positioned behind the deuterium bulb Two thin wires lead from the bottom of the unit to a socket on the electronic board 39 Light Sour
18. others more diffuse reflection The glossier the surface the more specular the reflection Reflection is expressed as a percentage R1 relative to the reflection from a standard reference substance S D R D where S is the sample intensity at wavelength A D is the dark intensity at wavelength A R is the reference intensity at wavelength R x 100 Common applications include measuring the reflection properties of mirrors anti reflection coatings and measuring the visual properties of the color in paints graphic arts plastics and food products To take a reflection measurement using OOIBase32 our spectrometer operating software follow these steps 1 Make sure you are in scope mode by either clicking the scope mode icon on the toolbar or selecting Spectrum Scope Mode from the menu Make sure the signal is on scale The peak intensity of the reference signal should be about 3500 counts During a reflection measurement keep the angle and distance from your reference and sample to your collection optics the same 2 Take a reference spectrum by first making sure nothing is blocking the light path going to your sample The analyte you want to measure must be absent while taking a reference spectrum Take the reference reading by clicking the store reference spectrum icon on the toolbar or selecting Spectrum Store Reference from the menu This command merely stores a reference spectrum You must select File Save Ref
19. your serial number spec You will then be asked if you would like to make this file the default spectrometer configuration file Choose Yes The next time you run OOIBase32 the software will use the file as the standard for your spectrometer configuration When you exit OOIBase32 any changes to the configuration file will be automatically saved to the default file 00lBase32 Settings Dialog Box At this point it is a good idea to configure several OOIBase32 operation parameters Choose Edit Settings from the menu to open the OOIBase32 Settings dialog box Go through each page of this dialog box to select options for saving opening and printing data to configure default setting files and to select other important options such as storing and copying data and choosing warning messages See the OOIBase32 Spectrometer Operating Software Manual for details Configure Data Acquisition Dialog Box Finally select Spectrum Configure Data Acquisition from the menu to set your data acquisition parameters in the Configure Data Acquisition dialog box The Basic page allows you to set the integration time and choose averaging and boxcar smoothing values The External Trigger page allows you to specify the external trigger mode The Strobe page allows you to control external strobe events with the spectrometer See the OOIBase32 Spectrometer Operating Software Manual for details Specifications Board architecture design h
20. 1 2 of JB to disable the tungsten bulb Using Solarization resistant Fibers Our 300 um Diameter Solarization resistant Optical Fiber consists of a silica core surrounded by a silica cladding material The fiber is then coated in aluminum Our solarization resistant fibers are best used for regions below 250 nm or for applications where exposure to long term UV light occurs Solarization is the loss of transparency in glass due to exposure to ultraviolet radiation If you are using a UV light source the UV radiation degrades the silica in a standard patch cord fiber over time resulting in increased overall absorption values and invalid data Replacing the Bulbs The deuterium and tungsten bulbs cannot be replaced by unauthorized personal To replace a bulb in the MINI D2T contact Ocean Optics Specifications Deuterium wavelength range 200 400 nm Tungsten wavelength range 360 850 nm Deuterium bulb life 800 hours Tungsten bulb life 2 000 hours Ignition delay 1 5 seconds to 60 seconds 0 3 in 30 minutes Peak to peak stability 1 0 in 10 minutes Connector SMA 905 Power input 12 V Power requirement 12 VDC 420 mA Power consumption 5 watts deuterium 3 8 watts tungsten 1 2 watts 35 F Light Sources D 1000 Wee D 1000 Deuterium Light Source The D 1000 DEUTERIUM LIGHT SOURCE is a high output high stability fiber optic light source optimized for the UV The D 1000 produc
21. Address Settings for the ADC500 cccceececeeeeeeeeeeneeeceeeeeeseaeeseeeeeesaaeeseeeeeesnaeeeseneees 98 Interrupt Request Settings for the ADC500 eseceeeeeeeeeeeeeeeeeeeeeeeeeeeeeseaeeeseaeeeseeeeeneeeee 99 Base Address Settings for the ADC1000 and PC2000 eececceeeceeeeeeeeeeeeeteeeeeeeeneeeeeaees 100 Interrupt Request Settings for the ADC1000 and PC2000 c eceeeeeseeeeeeeeeeeeteeeeeees 101 Appendix B Adjusting the Focus of a Collimating Lens 102 Appendix C Calibrating the Wavelength of the Spectrometer 103 Appendix D S2000 Pin outs and Jumpers 00 cee eee teeeeeteeees 105 Appendix E PC2000 Pin outs and Jumpers eect 108 Appendix F External Triggering 0 2 2 0 cece cece eeeeetee eects tteeeeteeeeeenes 109 ean PLCS inc F Introduction Ocean Optics miniature fiber optic spectrometers and accessories have revolutionized the analytical instrumentation market by dramatically reducing the size and cost of optical sensing systems More than 15 000 Ocean Optics spectrometers have been sold worldwide striking evidence of the far reaching impact of low cost miniature components for fiber optic spectroscopy Diverse fields such as research and development industrial process control medical diagnostics and environmental monitoring have benefited from access to Ocean Optics technology In the Beginning Ocean Optics began in 1989 when Florida university
22. Channel A through F for the First Channel Select the mathematical Operation to produce the data for Combination 1 Specify Time Channel A through F for the Second Channel Select a multiplicative Factor to apply to the data before it is plotted or stored Select an additive constant or Offset to apply to the data after the factor is applied and before the data is plotted or stored The equation for the Factor and Offset functions is Results Factor x Data Offset eee e ee 5 If desired configure a time acquisition process for the Combination 2 page It is almost identical to the Combination page except that you have the option of choosing Combination 1 for your First Channel or Second Channel 6 Click the OK button for the Time Acquisition Channel Configuration dialog box 96 First in Photonics ean Experiment Tutorial Time Acquisition CO 7 Access the Time Acquisition Time Acquisition Configuration Configuration dialog box by selecting M Stream Data to Disk M Show Values in Status Bar Time Acquisition Configure Configure Acquisition from the menu Write Data to Disk Every 100 Acquisitions Enable Stream Data to Disk to save time acquisition data peream c Program Files OOIBase32 Kinetics time El lename Enter a value in the Write Data to Disk Every X Acquisitions box to set M Save Full Spectrum with Each Acquisition the frequency for saving data The T Save Every Acquisition I Continue Unti
23. D sampling frequency 500 kHz maximum Communication port RS 232 Baud rate 2400 115 200 Input voltage 10 24V Input current 130 mA without spectrometer Interface cable 6 pin DIN connector to PC 25 pin connector to spectrometer Multiple channel capability supports up to 8 spectrometer channels Qnartrnamaotaor intanratinn tima R millieannnde ta RN cannnde QONNN enactramatare 25 F A D Converters DAQ 700 P DAQ 700 PCMCIA A D Converter The DAQ 700 PCMCIA A D CONVERTER is a 12 bit analog to digital converter card that connects our spectrometers to notebook PCs This 16 channel single ended 8 channel differential card also known by its National Instruments DAQCard 700 designation fits into a credit card size slot in a notebook PC It has a 100 kHz sampling frequency Before using your spectrometer you must first configure your computer to properly detect and use the DAQ 700 and then follow several steps to use it as an interface to your Ocean Optics spectrometer The following are directions for setting up your DAQ 700 Because A D converter installation goes hand in hand with software installation you will find directions for installing OOIBase32 Spectrometer Operating Software included in this section as well Install OOIBase32 Before installing OOIBase32 make sure that no other applications are running 1 Execute Setup exe At the Welcome dialog box click Next gt 2 At the Destination Location dial
24. Interface page enter the same Base Address 1 0 Range 769 0 0300 gt values as you did in the Configure Hardware x i 800 040320 dialog box l S cco The Detector Linearity page in this dialog 832 040340 e o 848 0x0350 box allows you to enter coefficients for an 864 0x030 Z algorthim that corrects for rare occurances of non linearity of the detector Contact Ocean Optics for more information Save the spectrometer configuration file by Cancel Apply Help choosing Spectrometer Save Configuration As from the OOJBase32 menu You can rename the file or use the default file name your serial number spec You will then be asked if you would like to make this file the default spectrometer configuration file Choose Yes The next time you run OOJBase32 the software will use the file as the standard for your spectrometer configuration When you exit OOIBase32 any changes to the configuration file will be automatically saved to the default file OOIBase32 Settings Dialog Box At this point it is a good idea to configure several OOIBase32 operation parameters Choose Edit Settings from the menu to open the OOIBase32 Settings dialog box Go through each page of this dialog box to select options for saving opening and printing data to configure default setting files and to select other important options such as storing and copying data and choosing warning messages See the OOIBase32 Spectrometer Operating Software Manual for de
25. JA of the ISS UV VIS s circuit board controls the deuterium bulb Short the pins 2 3 that is place a jumper over pins 2 3 of JA to enable the deuterium bulb Short the pins 1 2 that is place a jumper over pins 1 2 of JA to disable the deuterium bulb Disabling the Tungsten Bulb Jumper block JB of the ISS UV VIS s circuit board controls the tungsten bulb Short the pins 2 3 that is place a jumper over pins 2 3 of JB to enable the tungsten bulb Short the pins 1 2 that is place a jumper over pins 1 2 of JB to disable the tungsten bulb Using Solarization resistant Fibers For any experiments using UV light we recommend using our solarization resistant fiber Our 300 um Diameter Solarization resistant Optical Fiber consists of a silica core surrounded by a silica cladding material The fiber is then coated in aluminum Our solarization resistant fibers are best used for regions below 250 nm or where exposure to long term UV light occurs If you do not use solarization resistant fibers when using a UV light source the UV radiation degrades the silica in a standard patch cord fiber over time resulting in increased overall absorption values and invalid data Specifications Deuterium wavelength range 200 400 nm Tungsten wavelength range 360 850 nm Deuterium bulb life 800 hours Tungsten bulb life 2 000 hours Ignition delay 1 5 seconds to 60 seconds 0 3 in 30 minutes Peak to peak stability 1 0 in 10 minutes
26. LS 450 board see the JP2 table for pulse rates Using JP3 on the S2000 You can also have functionality of the R LS 450 by utilizing Jumper Block 3 JP3 on the S2000 circuit board The S2000 s JP3 allows you to control the R LS 450 through the OOIBase32 software Continuous Wave Mode with the 2000 s JP3 You can control the turning on and off of the R LS 450 while it is in continuous wave mode through the Strobe Enable function in the OOIBase32 software if The switch is turned to continuous wave mode There is a jumper over the pins in JP1 of the R LS 450 There is a jumper over the CW pins in JP2 of the R LS 450 There is a jumper over the Remote pins in JP3 of the R LS 450 There is a jumper over the 2 pins in JP3 of the S2000 board eee ee Pulsed Mode with the 2000 s JP3 Another way you can control the pulses per second of the R LS 450 is if The switch is turned to pulsed mode There is a jumper over the pins in JP1 of the R LS 450 There is a jumper over the CS pins in JP2 of the R LS 450 There is a jumper over the over the Remote pins in JP3 of the R LS 450 There is a jumper over pins labeled 16 14 12 or 10 in JP3 of the S2000 depending on the pulse rate you need The pulses per second are also dependent upon the frequency of your A D card See the table below for choices eee ee Pins on the S2000 s JP3 DAQ700 ADC500 SAD500 ADC1000 16 1 5 7 6 15 2 14 6 1 30 4 60 8 12 24 0 12
27. and the same available setting you found in your computer Spectrometer Type sel 52000 Pc2000 7 Pixel Remember that the Input output Range Base Address A D Conwerter Type 50 you selected was expressed in hexadecimal In this aoc1oo0Pc200 Sd 000 PC2000 dialog box the base address is given in decimal followed by the hexadecimal equivalent in parenthesis Base Address 1 0 Range IRQ Interrupt Request For example 768 0x0300 gives the base address as 768 0x0300 768 decimal and 300 hexadecimal ae fi x 4 Under IRQ choose the same setting as the dip switches on the A D board and the same available AD500 Pixel Resolution setting you found in your computer 1 5 For your setup only these parameters apply Ignore the other settings they apply to other A D converters Cancel Click OK You can always change these settings once OOIBase32 is fully operational by selecting Spectrometer Configure A D Interface f If you do not see the Configure Hardware screen exit the software Then select Start Run and type C windows ooidrv ini for Windows 95 98 systems or c winnt ooidrv ini for Windows NT systems Notepad will open Edit this file for our device driver by finding the Initialized entry and making sure this line reads Initialized 0 Save the OOIDRV INI file and exit Notepad Restart OO Base32 You should now see the Configure Hardware dialog box 21 First in P e
28. are using a fiber and cosine corrector with the lamp remove the inner barrel from the SMA connector Screw the CC 3 cosine corrector onto the end of the fiber The connection should be tight Insert the CC 3 fiber all the way into the LS 1 CAL s SMA connector taking the place of the SMA s inner barrel 3 Use an Allen wrench to tighten the set screw on the SMA connector of the LS 1 CAL Take the other end of the fiber and screw it all the way into the SMA connector of the spectrometer 4 Plug the switching AC adapter the black rectangle shaped box into the back of the LS 1 CAL The adapter stabilizes the power coming into the lamp to insure constant spectral intensity 5 Plug the power cord into a standard outlet Plug the other end of the power cord into the back of the switching AC adapter eae 52 an 10 11 12 13 14 15 16 17 18 F Light Sources LS 1 CAL piisi NO Find the on off switch on the back of the lamp and turn the lamp on Let the lamp warm up for at least 15 minutes before using Insert the disk that came with your lamp into your computer The disk contains two ASCII files These files have the same information as the Lamp Calibration Reports that came with your LS 1 CAL One file has the calibration numbers for calibrating the spectral response of your system with the lamp and a bare fiber its name contains the lamp s serial number followed by FIB LMP The second file has the cal
29. argon peaks Mercury Lines Argon Lines 4500 253 65 696 54 296 73 706 72 4000 302 15 710 75 3500 313 16 727 29 gt 334 15 738 40 a 3000 365 01 750 39 S 404 66 763 51 E 2500 407 78 772 40 a 2000 435 84 794 82 546 08 800 62 2 1500 576 96 811 53 i 579 07 826 45 1000 842 46 500 852 14 866 79 0 912 30 200 400 600 800 1000 92249 Wavelength nm Specifications Output low pressure gas discharge lines of Mercury and Argon Spectral range 253 922 nm Dimensions 11 4 cm x 6 98 cm x 2 54 cm LWH 4 5 x 6 98 x 1 0 LWH Power requirements 12 VDC wall transformer comes with unit or 9 VDC battery Internal voltage 600 volts at 30 kHz Bulb life 3 500 hours Amplitude stabilization 1 minute Aperture 3 mm Connector SMA 905 51 F Light Sources LS 1 CAL a LS 1 CAL Calibrated Light Source The LS 1 CAL CALIBRATED LIGHT SOURCE for the VIS Shortwave NIR 300 1050 nm is a tungsten halogen light source that provides you with known absolute intensity values at several wavelengths expressed in uW cm nm Since the spectral intensity of the LS 1 CAL can be traced to an intensity standard provided by the National Institute of Standards and Technology NIST it is specifically designed for calibrating the absolute spectral response of your system Parts Included LS 1 CAL Calibrated Light Source Switching AC adapter for stabilizing power Power cord for supplying power to the
30. below After finding the A D converter you are using choose your integration time You have four choices S2000 Miniature Fiber Optic Spectrometer Integration Time with Integration Time with Integration Time with Jumper Block 4 JP4 DAQ700 ADC500 SAD500 ADC1000 Pins 1 2 Pins 3 4 27 5 ms open shorted 55 0 ms shorted open 137 5 ms shorted shorted 111 First in Photonics CO Appendix F PHICS inc 4 Once you have selected the integration time note the configuration of the pins in the Jumper Block 4 column of the chart JP4 5 Remove your spectrometer from its housing Do not tamper with the optical bench If you have more than one channel in your system you may have to disconnect the channels from one another The master spectrometer is usually on the bottom of a multiple channel system In the center of the green circuit board near the optical bench find Jumper Block 4 labeled JP4 See the figure to the right Jumper Block 4 consists of the first four pins which are numbered 1 2 3 and 4 Using jumpers configure the pins to match the integration time you selected See chart on previous page N A T x d D A N A NEU NI o ees NE NI 3 For example You have an S2000 and an ADC1000 A D converter Out of your four choices for an integration time you select 13 3 milliseconds Pins 1 2 must be open and Pins 3 4 must be shorted Leave Pins 1 2 uncovered and plac
31. collimating lenses in the illumination part of your setup Continue to adjust the focus of the other collimating lenses in your setup If the read fiber is the same diameter size as the illumination fiber repeat step 6 with each collimating lens in your setup If the read fiber is a different diameter size than that of the illumination fiber you need to remove the illumination fiber from the light source connect the read fiber to the light source and repeat step 6 for every collimating lens on the read part of your setup 102 ean PtiCS me F Appendix C Calibrating the Wavelength of the Spectrometer The following describes how to calibrate the wavelength of your spectrometer Though each spectrometer is calibrated before it leaves Ocean Optics the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions You are going to be solving the following equation which shows that the relationship between pixel number and wavelength is a third order polynomial A Cp F C p Cp where is the wavelength of pixel p J is the wavelength of pixel 0 C is the first coefficient nm pixel C is the second coefficient nm pixel2 and C is the third coefficient nm pixel3 You will be calculating the value for I and the three Cs Setting Up To re calibrate the wavelength of your spectrometer you will need the following A light source that produces spectral lines Oc
32. create a table like the one below In the first column place the exact or true wavelength of the spectral lines that you used The spectral lines of the HG 1 are printed on the lamp s casing In the second column of this worksheet place the observed pixel number In the third column calculate the pixel number squared and in the fourth column calculate the pixel number cubed Independent Dependent Values computed from Variable Variables the regression output True gt ae Pixel Pixel 2 Pixel Poa Difference 253 65 175 30625 5359375 253 56 0 09 296 73 296 87616 25934336 296 72 0 01 302 15 312 97344 30371328 302 40 0 25 313 16 342 116964 40001688 313 02 0 13 334 15 402 161604 64964808 334 19 0 05 365 02 490 240100 117649000 365 05 0 04 404 66 604 364816 220348864 404 67 0 01 407 78 613 375769 230346397 407 78 0 00 435 84 694 481636 334255384 435 65 0 19 546 07 1022 1044484 1067462648 546 13 0 06 576 96 1116 1245456 1389928896 577 05 0 09 579 07 1122 1258884 1412467848 579 01 0 06 696 54 1491 2223081 3314613771 696 70 0 15 706 72 1523 2319529 3532642667 706 62 0 10 727 29 1590 2528100 4019679000 727 24 0 06 738 40 1627 2647129 4306878883 738 53 0 13 751 47 1669 2785561 4649101309 751 27 0 19 4 Now you are ready to calculate the wavelength calibration coefficients In your spreadsheet program find the functions to perform linear regressions If you are using Quattro Pro look under Tools Advanced Math
33. detector efficiency in the spectrometer and attenuation by the fiber 1 Put on safety eyewear 2 Lift the black protective shutter covering the fiber optic port Remove the red plastic cap that covers the SMA connector and install an optical fiber For best results use our solarization resistant fibers Plug the power cord into the rear of the D 1000 Plug the other end into a 110 VAC outlet 4 Locate the black power switch at the rear of the D 1000 just above where the power cord connects to the D 1000 Turn on the power When the power is on the green Power On light located at the bottom right of the front panel will light 5 Push in the UV Start button located on the front panel This white button turns on the deuterium lamp Pushing in the UV Start button initiates the start up sequence for the lamp First the heater in the deuterium lamp ionizes the available deuterium At this point the yellow Heater On light on the front panel will light Next after 30 seconds the red UV On light located at the top of the front panel will light The Heater On light will turn off at the same time Once the red UV On light is lit the deuterium lamp is ready for your application Best results are obtained after the lamp has warmed up for 30 minutes 6 Without a solarization resistant fiber the spectral output will decrease significantly as a function of time at wavelengths lower than 250 nm 7 To turn off the deuterium lamp first press the wh
34. diameter solarization resistant fibers 1 illumin ation 1 read in a 5 0 x 0 25 OD stainless steel ferrule Screw on interchangeable probe tips in path lengths of 2 mm 5 mm or 10 mm are available to configure your system for either optically dense or dilute solutions Caution R Handle with care Dropping the instrument may cause permanent damage f4 Bubbles will interfere with your readings Regularly inspect the sample region for bubbles Operation The T300 consists of two identical fibers in a bifurcated assembly A plano convex lens shapes the light coming out of the illumination fiber The light is transmitted through the sample hits the mirror reflects off the mirror and interacts with the sample again before being transmitted back through the probe via the read fiber Because the light travels through the sampling region twice the optical pathlength is actually twice the length of the sample aperture The transmission cell is used to measure absorbance of the fluid that fills the sample compartment between the fibers and the mirror which is a UV enhanced aluminum second surface mirror 1 Connect one leg of the probe to the light source and connect the other leg to the spectrometer It does not matter which leg of the probe is connected to the light source or spectrometer 2 Make sure the probe tip you want to use for your experiment is screwed onto the end of the probe To replace the probe tip simply unscrew the probe tip and
35. flush with the inside of the holder of course the cuvette is absent If necessary adjust the mirrored screws so that they are flush with the inside of the holder Locate the two ball plunger screws Loosen the two ball plunger screws until the ball end of the screws is just visible in the cell holder Insert your cuvette into the holder Tighten the ball plunger screws until the ball contacts the cuvette Do not over tighten Br FO Installing Filters 1 Loosen the filter clamping screw with a screwdriver 2 Insert the filter into the filter slot The filter slot can accommodate filters up to 6 mm thick 3 Clamp the filter with the screwdriver 60 Sampling Chambers CUV FL DA ean lt tics Specifications Path length 1cm Dimensions 1 4 x 1 4 x 1 0 Lx WxH Material Black anodized aluminum Hole diameter for mounting to light source 0 375 Collimating lens Dynasil 1100 quartz 200 nm 2 um 5 mm diameter Collimating lens termination SMA 905 Filter slot accepts filters up to 1 4 6 mm in thickness Reflection mirrors UV enhanced aluminum coated for optimal signal reflection and collection Mirror diameter 0 3 7 5 mm Size of light beam reaching sample Y minimum divergent 61 F ean Sampling Chambers ISS ee ISS Integrated Sampling System The ISS INTEGRATED SAMPLING SYSTEM is a fully integrated 1 cm cuvette
36. hex head wrench not included The bars can be set far enough apart to accept samples up to 10 cm thick The base is scored at 1 2 cm intervals as a path length guide Specifications base is blue anodized aluminum Assembly material r y mount bars are black anodized aluminum base is 3 x 6 x 14 thick Dimensions mount bars are 1 wide x 0 3 thick entire assembly is 6 in height mounting bars accept 3 8 24 threads Threads set screws for mounting bars have 10 32 threads use 5 32 hex head wrench to loosen Measurement bar has Ye cm gradations as path length measurement guide total length is 10 cm 78 F Sampling Optics FVA UV O FVA UV Fiber Optic Variable Attenuator The FVA UV FIBER OPTIC VARIABLE ATTENUATOR is an opto mechanical device that controls the amount of light entering the optical bench of the spectrometer Each end of the attenuator has SMA 905 terminations for connecting the attenuator to light sources sample holders and fibers The FVA UV attenuates light uniformly at all wavelengths Application Tips The attenuator can be used for applications where more light reaches the spectrometer than likely can be digitized successfully by the spectrometer s high sensitivity linear CCD array detector Some absorbance experiments may also require signal attenuation as too much light can saturate the reference measurement In some instances detector saturation can be avo
37. is continually scanning and collecting data With each trigger the data collected up to the trigger event is transferred to the software If you continuously apply triggers such as by holding down the button on an external switch this mode is equivalent to operating in the Normal Mode In the Software Mode you set the integration time in the software It is the only external triggering mode where the integration time is set in the software All other acquisition parameters are set in the software as well The source for the integration clock comes from the A D converter Use the Software Mode if you are using a continuous illumination source and the light intensity is constant before during and after the trigger need to set the integration time in the software To Use the Software Mode 1 Supply a line from your triggering device to Pin 3 of the J2 Accessory Connector to provide the positive voltage 5 VDC to the spectrometer See figure above for pin location We do not advise using an outside 109 F Appendix F piles source to supply the voltage as it is based on a referenced ground and your reference may be different from ours Using Pin 3 to supply voltage ensures that the spectrometer will receive the appropriate voltage for the trigger event 2 Supply a line from Pin 8 of the J2 Accessory Connector to your triggering device See figure on page 109 for pin location 3 Set your acquisition parameters in the sof
38. is the reference intensity at wavelength A Common applications include measurement of transmission of light through solutions optical filters optical coatings and other optical elements such as lenses and fibers To take a transmission measurement using OOIBase32 our spectrometer operating software follow these steps 1 Make sure you are in scope mode by either clicking the scope mode icon on the toolbar or selecting Spectrum Scope Mode from the menu Make sure the signal is on scale The peak intensity of the reference signal should be about 3500 counts 2 Take a reference spectrum by first making sure nothing is blocking the light path going to your sample The analyte you want to measure must be absent while taking a reference spectrum Take the reference reading by clicking the store reference spectrum icon on the toolbar or selecting Spectrum Store Reference from the menu This command merely stores a reference spectrum You must select File Save Reference from the menu to permanently save the spectrum to disk Storing a reference spectrum is requisite before the software can calculate transmission spectra 3 While still in scope mode take a dark spectrum by first completely blocking the light path going to your sample If possible do not turn off the light source If you must turn off your light source to store a dark spectrum make sure to allow enough time for the lamp to warm up before continuing your experiment Take
39. jumpered pins on the spectrometer s circuit board 3 the data is transferred to the software 4 the spectrometer then sits idle waiting for the next trigger In the Hardware Mode you set the integration time by positioning jumpers over pins on the spectrometer s circuit board All other acquisition parameters are set in the software The source for the integration clock is the spectrometer This mode is ideal for use with laser and other short pulse events Use the Hardware Mode if you are using a pulsed excitation or light source in your experiment are doing LIF fluorescence with pulsed excitation or phosphorescence experiments are able to jumper the pins on Jumper Block 4 of the spectrometer s electronic board need to synchronize an acquisition with an external event bali dih ih d To Use the Hardware Mode 1 Supply a line from your triggering device to Pin 3 of the J2 Accessory Connector to provide the positive voltage 5VDC to the spectrometer See figure on page 109 for pin location We do not advise using an outside source to supply the voltage as it is based on a referenced ground and your reference may be different from ours Using Pin 3 to supply voltage ensures that the spectrometer will receive the appropriate voltage for the trigger event 2 Supply a line from Pin 4 of the J2 Accessory Connector to your triggering device See figure on page 109 for pin location 3 To set the integration time look at the charts
40. lens on the LS 1 turn to Appendix B Spectral Output These graphs represent normalized blackbody curves for tungsten halogen light sources with 2800K and 3100K color temperatures The observed spectral output of the LS 1 will vary due to bulb type the spectrometer configuration the sampling optics used and inherent fluctuations in LS 1 output 124 1 24 14 14 o co 1 Normalized Intensity emi t D A 1 Normalized Intensity oO D 5 N n o N o T T T T T T T 1 T T T T r T r 300 500 700 900 1100 1300 1500 1700 1900 300 500 700 900 1100 1300 1500 1700 Wavelength nm Wavelength nm 900 hour bulb 3100K 10 000 hour bulb 2800K Specifications 1900 Spectral range 360 nm 2 um Dimensions 9 0 cm x 5 0 cm x 3 2 cm LWH 3 5 x 2 0 x 1 25 LWH Power input 12 VDC 800 mA 7 20 VDC 0 5 2 amps Power output 6 5 watts Bulb life 900 hours standard 10 000 hours long life Bulb color temperature 900 hour bulb 3100K 10 000 hour bulb 2800K Output to bulb 5 volts 1 3 amps Output regulation 0 2 voltage Time to stabilized output 30 minutes Bulb output 7400 foot candles 7 4MSCP Connector SMA 905 Though the product can be used to 2 um it can be configured to see only to 1100 nm with our S2000 spectrometer 42 F Light Sources PX 2 piisi NO PX 2 Pulsed Xenon Lamp The PX 2 PULSED XENON LAMP is a high flash rate short
41. power is on the green Power On light located at the bottom right of the front panel will light At this time the user can now power up the deuterium or tungsten halogen source or both See the following Operating the Deuterium Source and Operating the Tungsten Halogen Source sections for more information 5 To turn off the DT 1000 both deuterium and tungsten halogen sources must be turned off first Only then can the user flip the power switch at the rear of the unit to the off position The green Power On light will turn off 6 Lift up the black protective shutter and disconnect your optical fiber from the SMA connector Oo 38 F Light Sources DT 1000 piisi NO Operating the Deuterium Source 1 To power the deuterium lamp push in the UV Start button located on the front panel This white button turns on the deuterium lamp Pushing in the UV Start button initiates the start up sequence for the lamp First the heater in the deuterium lamp ionizes the available deuterium At this point the yellow Heater On light on the front panel will light After 30 seconds the red UV On light located at the top of the front panel will light The Heater On light will turn off at the same time 2 To turn off the deuterium lamp press the white UV Off button located on the front panel The red UV On light should go out Operating the Tungsten Halogen Source 1 To power the tungsten halogen lamp push in the white button Visible On The
42. screw in either the 2 mm 5 mm or 10 mm replaceable tip Prepare your sample 4 While the probe tip is in the sample you should achieve a signal in Scope Mode for OOIBase32 software of 3500 counts To achieve the best signal use an Allen wrench to loosen the set screw on the inner fiber barrel assembly and slide it up and down to change the intensity of the light returned The inner barrel is set at the time of manufacture for a 10 mm tip in aqueous media If your application requires measuring gases you will have to adjust the inner barrel You may also have to adjust the integration time to achieve this signal Immerse the probe in distilled water or the solvent of your choice and take a reference spectra Remove the probe from the reference block the light path going to the spectrometer and take a dark spectrum 7 Make sure the light path is clear place the probe in your sample solution and take your sample spectra p DM Specifications Fiber core diameter 300 um Fiber material silica core cladding aluminum jacketing Fiber bundle 1 illumination fiber and 1 read fiber solarization resistant Fiber bundle length 2 meters breakout is 1 5 meters from probe tip Wavelength optimization 200 1100 nm Numerical aperture 0 22 Inner and outer ferrules Stainless steel Ferrule diameters 0 125 inner ferrule diameter 0 25 outer ferrule diameter Outer ferrule l
43. sure the First Coefficient Second Coefficient Third Coefficient and Intercept correspond to those of your system 3 Adjust your acquisition parameters using the Acquisition Parameters dialog bar or select Spectrum Configure Data Acquisition from the menu 4 Acquiring spectral data from your spectrometer is quite simple Assuming that you have followed the previous steps and started OOIBase32 your spectrometer is already acquiring data Even with no light in the spectrometer you should see a fluctuating trace near the bottom of the graph If you put light into the spectrometer you should see the graph trace rise with increasing light intensity If this occurs you have correctly installed your hardware and software 5 Once you installed and configured your hardware and software and have set up your system you are ready to take your measurements There are four basic optical measurements from which to choose absorbance transmission reflection and relative irradiance measurements The type of measurements you wish to make determines the configuration of the sampling optics for your system The choice of reference and data analysis determines how the answer is presented 6 For each measurement a reference and dark spectrum must first be made After taking a reference and a dark spectrum you may take as many absorbance transmission reflection or relative irradiance measurement scans as you want However if at any time any sampling va
44. to 2 um it can be configured to see only to 1100 nm with our S2000 spectrometer 75 F Sampling Optics 74 90 UV O 74 90 UV Right Angle Reflector The 74 90 UV RIGHT ANGLE REFLECTOR is a 3 8 24 threaded black anodized aluminum assembly for mounting collimating lenses at right angles and is useful for applications involving limited space and inconvenient optical fiber routing Application Tips The 74 90 UV Right Angle Reflector has a plane mirror located under its cap that reflects light from the collimating lens to 90 This mirror is coated with a UV enhanced aluminum substrate that is gt 90 reflective from 200 1100 nm The 74 90 UV has two 3 8 24 threaded ports at top and bottom that accommodate collimating lenses The top port has a 3 8 x 1 threaded nipple that can be removed for connecting to a male adapter Using the 74 90 UV is easy Screw in a collimating lens into a port and attach a fiber to the collimating lens For directions on adjusting the focus of a collimating lens see Appendix B Specifications Assembly material black anodized aluminum Dimensions 0 65 x 065 x 0 787 LWH Mirror coating UV enhanced aluminum Mirror reflectivity gt 90 from 200 1100 nm 3 8 24 ports UEa 3 8 x 1 nipple 76 ean Sampling Optics 74 OPM Orr F 74 OPM Optical Post Mount The 74 OPM OPTICAL Post MOUNT is a 3 8 24 threaded black anodized aluminum assembly used for m
45. 0 counts The light source must be a blackbody of known color temperature 2 Take the reference reading by clicking the store reference spectrum icon on the toolbar or selecting Spectrum Store Reference from the menu This command merely stores a reference spectrum You must use the Save Reference command to permanently save the spectrum to disk Storing a reference spectrum is requisite before the software can calculate relative irradiance spectra 3 While still in scope mode take a dark spectrum by completely blocking the light path If possible do not turn off the light source Take the dark reading by clicking the store dark spectrum icon on the toolbar or selecting Spectrum Store Dark from the menu This command merely stores a dark spectrum You must use the Save Dark command to permanently save the spectrum to disk Storing a dark spectrum is requisite before the software can calculate relative irradiance spectra 4 Take a relative irradiance measurement by first positioning the fiber at the light source you wish to measure Then choose the irradiance mode icon on the toolbar or select Spectrum Relative Irradiance Mode from the menu In the Reference Color Temperature dialog box enter the light source s color temperature in Kelvin and click OK To save the spectrum click the save icon on the toolbar or select File Save Processed from the menu f If at any time any sampling variable changes including integration time aver
46. 0 i Ai computer is using to interface to your SAD500 See the A D Converter Type 350 Troubleshooting section to determine the COM Port sao YS Under Baud Rate select the speed at which the SAD500 will operate We recommend using 115 200 baud rate foe Address ee ee ees Under SAD Pixel Resolution enter resolution values from 768 0 0300 zl f F 1 to 500 This value specifies that every n pixel of the IER Baud Rate spectrometer is transmitted from the SAD500 to the PC fi zl s20 Your resolution value depends on your experiment By SAD500 Pixel Resolution sacrificing resolution you gain speed The transfer of one mm Compress SAD500 Data complete spectra requires 0 4 seconds when communicating at 115 200 baud rate If you need your information in lt 0 4 seconds increase the resolution or enable data compression see below Enable the Compress SAD500 Data function to minimize the amount of data transferred over the RS 232 connection Transmission of spectral data over the serial port is a relatively slow process Enabling this function ensures that every scan transmitted by the SAD500 will be compressed greatly increasing the data transfer speed of the SAD500 For your setup only these parameters apply to your system Ignore the other settings they apply to other A D converters Click OK You can always change these settings once OOJBase32 is fully operational by selecting Spectrometer Configure A D Interface Cancel
47. 0 nm 2 um 9 0 cm x 5 0 cm x 3 2 cm LWH Dimensions 3 5 x 2 0 x 1 25 LWH Power input 12 VDC 800 mA 7 20 VDC 0 5 2 amps Power output 6 5 watts Bulb life 10 000 hours Bulb color temperature 2800K Output to bulb 5 volts 1 3 amps Output regulation 0 2 voltage Time to stabilized output 30 minutes Bulb output 7400 foot candles 7 4MSCP The useable range of the ISS sampling device is limited to the wavelength range of the spectrometer to which it is coupled i e though ISS lens is optimized for use to 2 um they can be configured to see only to 1100 nm with our spectrometers 63 F ean Sampling Chambers ISS 2 Wee ISS 2 Integrated Sampling System The ISS 2 INTEGRATED SAMPLING SYSTEM is a fully integrated 1 cm cuvette holder and tungsten halogen light source It couples to our spectrometers with optical fiber to create a small footprint systems for VIS NIR 360 1100 nm The ISS 2 has a 900 hour bulb Also in the ISS 2 the fan is not enclosed in the base it is exposed and should be handled with care Parts Included ISS 2 cuvette holder and light source assembly 12VDC wall transformer for providing power l cm square plastic cuvette for holding samples Screwdriver for adjusting the fit of the cuvette Allen wrench for adjusting the collimating lens eee o o Operation Attaching the Fibers 1 Attach a SMA terminated optical fiber to the
48. 2 0 244 0 10 98 0 488 0 976 0 47 ean PtiCS me Light Sources R LS 450 You can control the pulses per second of the R LS 450 through the Flash Delay function in the OOIBase32 software if The switch is turned to pulsed mode There is a jumper over the pins in JP1 on the R LS 450 board There is a jumper over the pins labeled CS in JP2 on the R LS 450 board There is a jumper over the pins labeled Remote in JP3 on the R LS 450 board There is a jumper over the pins labeled 2 in JP3 on the S2000 board eee i a Setting the Integration Time When using any of the pulsed modes for the R LS 450 you need to ensure that a constant number of flashes occur for every integration cycle This achieves a continuous and stable signal The integration time is set in the OOIBase32 software To achieve a constant number of flashes per integration cycle the integration time must be a multiple of those shown in the following table according to the A D converter being used Pins on the JP3 Integration time for Integration time for Integration time for of the 2000 DAQ700 must be ADC500 SAD500 must be ADC1 000 must be multiple of multiple of multiple of 16 512 128 au 14 128 32 16 M12 32 8 4 10 8 with a min value of 24 ms 4 4 2 N A N A N A 48 F Light Sources HG 1 piisi NO HG 1 Mercury Argon Calibration Source The HG 1 MERCURY ARGON CALIBRATION SOURCE is a wavelength calibrat
49. 20 0x320 faswaon 1 Example 816 decimal Hex330 0x330 passon e a S S e switch is in the on upward position 98 CO Appendix A oe Interrupt Request Settings for the ADC500 To change the IRQ settings on the ADC500 board see the bank of 4 dip switches labeled SW2 In the default setting the IRQ is set to 7 Other combinations for IRQ settings are below After you have changed the switches reinstall the card and change the software settings to match the hardware settings See pages 15 18 for instructions The gray block indicates the position of the switch Interrupt Request 3 switch is in the on upward position 99 CO Appendix A ee Base Address Settings for the ADC1000 and PC2000 To change the Base Address settings on the ADC1000 and PC2000 see the bank of switches on the A D board The Base Address may be changed via the first 6 switches the IRQ may be changed via the last 3 switches Switches in the OFF position have the decimal values shown Switches in the ON position have a value of zero The Base Address is the sum of the values of the switches In the default setting switches 5 and 6 are added to give a total of 768 A few of the many combinations for Base Address settings are below After you have changed the switches reinstall the card and change the software settings to match the hardware settings See pages 19 22 for instructions The gray block indicates the pos
50. 3 Attach the read leg to the spectrometer 4 Using the RPH 1 Reflection Probe Holder or some other holding device point the probe at the surface to be measured 5 The distance from the probe tip to the sample directly affects the signal For quantitative results the distance and angle must be held constant Specifications Fiber core diameter 200 um or 400 um Fiber core cladding silica Fiber bundle 6 illumination fibers around 1 read fiber Numerical aperture 0 22 Optimization UV VIS 200 750 nm and VIS NIR 450 1000 nm Ferrule Stainless steel or plastic Ferrule dimensions 3 0 x 0 25 Terminations SMA 905 Sheathing Blue PVC with Kevlar reinforcement Temperature range 20 C to 80 C Probe assembly length 2 meters breakout is halfway custom probes are also available 84 F ean Fiber Optic Probes and Accessories RPH 1 Per RPH 1 Reflection Probe Holder The RPH 1 REFLECTION PROBE HOLDER is an anodized aluminum platform with machined holes at 45 and 90 to hold our R200 Reflection Probes or other 0 25 O D probes during reflection measurements Common applications include measuring the reflection properties of mirrors and anti reflection coatings and measuring the visual properties of color in paints graphic arts plastic and food products Operation Reflection is the return of radiation by a surface without a change in wavelength The reflection may be
51. Caution xe The beam emerging from the DT 1000 produces visible light and or invisible ultraviolet radiation Direct contact with the beam could cause serious eye injury Safety eyewear must be worn at all times while operating the DT 1000 Do not remove any safety device installed Xa Dangerous voltages present Only qualified service personnel should service the DT 1000 This instrument should not be used for any clinical or diagnostic purposes Handle with care Dropping the instrument may cause permanent damage Gada xe For optimum performance below 250 nm use a solarization resistant fiber with this lamp Operation Allow several minutes for the lamp to warm up and for the power to stabilize regardless of the bulb being used Best results are obtained after 30 minutes The spectral output as delivered by an optical fiber will decrease significantly at wavelengths lower than 250 nm due to attenuation in the lamp envelope the decrease in detector efficiency in the spectrometer and attenuation by the fiber 1 Put on safety eyewear 2 Lift the black protective shutter covering the fiber optic port Install an optical fiber For best results use our solarization resistant fibers Plug the power cord into the rear of the DT 1000 Plug the other end into a 110 VAC outlet 4 To turn on the power for the DT 1000 flip the power switch at the rear of the unit just above where the power cord connects to the DT 1000 When the
52. Chemical resistance and Perclene Recommended optical fibers for coupling to 400 um illumination fiber UV VIS spectrometers light sources 200 um or 400 um read fiber UV VIS Plumbing fittings standard 1 4 x 28 chromatography fittings 71 F Sampling Chambers CUV CCE a CUV CCE Electrophoresis Sample Cell The CUV CCE ELECTROPHORESIS SAMPLE CELL for chromatography and capillary electrophoresis is an optical fixture for measuring the UV absorbance of fluids in chromatography or capillary electrophoresis systems The cell is attached on line i e the light is projected through the sides of fused silica tubing without violating the tube integrity For this reason there are no pressure limitations associated with the device The cell can accommodate fused silica tubing up to 500 um in diameter The user must provide a clear optical window For standard polyimide jacketed tubing this can be accomplished by burning off a short section of the jacketing The CUV CCE comes with F230 0 016 ID tubing sleeves If your tubing is a different size you can order sleeves with different inner diameters The cell fixture is made from a standard 10 32 PEEK Cross Upchurch P 729 with a 0 02 through hole 10 32 coned female threads and 4 F 300 PEEK finger tight fittings The optical fibers are aluminum jacketed 300 um diameter solarization resistant silica core silica clad UV waveguides The optical fibers are inserted fa
53. D Converter c ccceceececeeteeteeeeeeeeeeaeeeeeeeeecaeeeeeeeeescaeeeseaeeeteeeeseneeeee 26 OOIBase32 Operating Software ne ce cc cece eee tees eeeeeeeeeneeeneeeneeneesneees 31 Light SUC S ccs ee ie ee a 33 MINI D2T Miniature Deuterium Tungsten Light SOUICE c ccceeeeeeeeeeeeeeteeeeeeeeesseeeeeneees 34 D 1000 Deuterium Light SourGe sinc nici ieene ennai bade 36 DT 1000 Deuterium Tungsten Halogen Light SOUrCE c cceeceeeeeeeeeecteeeeeeeseeeeeeetaeeeees 38 LS 1 Tungsten Halogen Light SOUC ss 41 PX 2 PulSed XENON Lamp seeriaid ananuna andaeni dadian idais laani dennda tnieda unadai 43 LS 450 Blue LED Pulsed Light SOU CS irresirrancsna a a A N T 45 R LS 450 Rack mount Blue LED Pulsed Light Source ssssseeeeesesssresssreereerrrseresrreens 46 HG 1 Mercury Argon Calibration SOUrCE siinne 49 LS 1 CAL Calibrated Light SOUrCC eccececeeeeceeneeeeeeeeeeeaaeeseeeeesaaeeseeeeeesaaaesseneeesnaaeeseaeees 52 Sampling Chambers tants dctcaeceect cc ethseeseactnticnakte nected creeds seeds 54 CUV UV CUV VIS Cuvette Holders cccccccccccccccccececeesesesaeeeeueeeeeeseceeeeeseceeeeeeeeeeeeeeneneneaea 55 CUV UV 10 CUV VIS 10 Cuvette Holders cccccccceseeeseeeeeeeeeseseeeeeceeceeeeeeeeeeeeeeeeeeneaea 57 CUV ALL 4 way Cuvette Holder ccecccecccceceeceeeeceeeeeeaaeeeeaeeecaaaeseeaneeseaaeeeseeeeeecaeeeeeaaeeneaes 58 CUV FL DA Direct Attach Cuvette Holder eccccececeeeeeeeececaeeeeaeeesecaeeesaeesecue
54. I D2T above the 12V label For users of European version wall transformers plug the transformer into a standard 220 V outlet At this time the green LED indicator light on the front of the lamp will be lit This indicator light only means that the lamp is receiving power not that the deuterium and tungsten bulbs are on Operating the MINI D2T Manually 1 Find the switch on the back of the MINI D2T 2 There are three positions On Off and Remote For Manual operation move the switch to the On position There can be up to a 1 5 second delay between switching the lamp to on and the bulbs igniting If the lamp has not been used recently the deuterium bulb may take up to 60 seconds to ignite 3 For 0 3 peak to peak stability allow 30 minutes warm up time before taking your measurements Operating the MINI D2T through Software 1 Take the 15 pin accessory cable and plug one end into the spectrometer Connect the other end of the accessory cable into the back of the MINI D2T 2 Find the switch on the back of the MINI D2T There are three positions On Off and Remote For Software operation move the switch to the Remote position Moving the switch to the Remote position enables you to control the lamp through the software whether the software you are using is OOIChem or OOIBase32 3 When using OOIChem select Spectrometer Enable Strobe from the menu to turn the MINI D2T on and off 34 F Light Sources MINI D2T phis NO 4 Whe
55. If you are using Excel look under Tools Data Analysis 5 Select the true wavelength as the dependent variable Y Select the pixel number pixel number squared and the pixel number cubed as the independent variables X After you execute the regression an output similar to the one shown below is obtained Regression Statistics Multiple R 0 999999831 R Square 0 999999663 Adjusted R Square 0 999999607 Standard Error 0 125540214 Observations 22 intercept Coefficents Standard Error Intercept 190 473993 0 36904 first coefficient X Variable 1 0 36263983 0 001684745 X Variable 2 1 174416E 05 8 35279E 07 X Variable 3 2 523787E 09 u ee second coefficient third coefficient A 50 Light Sources HG 1 ean PLCS inc F 6 The numbers of importance are indicated in the above figure You will need to record the Intercept as well as the First Second and Third Coefficients Also look at the value for R squared It should be very close to 1 If it is not you have probably assigned one of your wavelengths incorrectly 7 Select Spectrometer Configure from the menu and choose the Wavelength Calibration page to update the wavelength coefficients within OOIBase32 8 Repeat this process for each channel in your setup Spectral Output Mercury emission lines are lt 600 nm Argon emission lines are gt 600 nm and are shown here on the right on an exaggerated amplitude scale Below is a list of the most prominent mercury and
56. Operating Manual and User s Guide S2000 Miniature Fiber Optic Spectrometers and Accessories Offices East Coast 380 Main Street Dunedin Fla USA 727 733 2447 727 733 3962 Fax 8 30 a m 6 p m EST West Coast 5190 Golden Foothill Parkway El Dorado Hills Calif USA 916 939 4300 916 939 4307 Fax Noon 9 p m EST Ocean Optics B V Europe Nieuwgraaf 108 G 6921 RK DUIVEN The Netherlands 31 0 26 3190500 31 0 26 3190505 Fax E mail Info OceanOptics com general sales inquiries Info OceanOpticsBV com for sales questions in Europe Orders OceanOptics com for questions about orders TechSupport OceanOptics com for technical support For all your photonice needs visit OceanOptics com First in Photonics Copyright 2000 Ocean Optics Inc All rights reserved No part of this publication may be reproduced or transmitted in any form or by any means electronic or mechanical including photocopy recording or stored in a retrieval system without written permission from Ocean Optics Inc This manual may be saved on the customer s PC and may be printed in sufficient quantities for those using and operating Ocean Optics products only and not for resale This manual is sold as part of an order and subject to the condition that it shall not by way of trade or otherwise be lent re sold hired out or otherwise circulated without the prior consent of Ocean Optics Inc in any form of binding or cov
57. Sources LS 1 piisi NO LS 1 Tungsten Halogen Light Source The LS 1 TUNGSTEN HALOGEN LIGHT SOURCE is a versatile white light lamp utilized for the VIS Shortwave NIR 360 nm 2 um The lamp available with either a 900 hour or 10 000 hour bulb offers high color temperature and extremely efficient output Parts Included LS 1 Tungsten Halogen Light Source 12 VAC power supply Allen wrench for adjusting the collimating lens ee Caution DO NOT insert plastic or flammable materials into the filter slot The materials could melt or ignite The unit could ignite flammable materials that come in contact with the metal housing xa Xe The LS 1 becomes HOT during operation No cooling fan is installed in the LS 1 Handle with care Operation Turning On the Lamp Plug the wall transformer end into a standard 110 V outlet Plug the 12 V output end into the back of the LS 1 Screw a fiber into the SMA connector on your LS 1 Find the on off switch on the back of the lamp and turn the lamp on Allow the lamp to warm up for approximately 30 minutes Yor bee Using the Filter Slot The slot between the lamp and the fiber coupler can be used to hold filters or light blocks You can place a filter into the filter slot however be aware of the following The filter slot accepts filters up to 3 mm thick Because the lamp can become hot avoid plastic filters as they may melt The unit could ignite flammable materials that come in co
58. Specular in which the angle of incidence is equal to the angel of reflection If taking specular reflection measurements position the reflection probe in the 90 aperture of the RPH 1 Diffuse in which the angle of incidence is not equal to the angle of reflection If taking diffuse reflection measurements position the reflection probe in the 45 aperture of the RPH 1 Every surface returns both specular and diffuse reflections Some surfaces may return mostly specular reflection others more diffuse reflection The glossier the surface the more specular the reflection Specular Reflectance Measurements For a specular reflection measurement attach the illumination leg of your reflection probe to a light source and the read leg to the spectrometer Place the end of the probe in the 90 aperture of the RPH 1 Use the cap screw on the holder to secure the probe at the desired distance from the sample 1 First take a reference spectrum Make sure nothing is blocking the light path going to your reference Place the reflection probe probe holder over a first surface mirror Take the reference reading 2 Next take a dark spectrum Completely block the light path going to your sample Do not turn off the light source Take the dark reading 3 Finally take your reflection measurement Make sure the sample is in place and nothing is blocking the light going to your sample If using OOIBase32 click on the Transmission icon to take your spec
59. a time acquisition process H There are options to either store data for each acquisition or to collect data only after a specified delay Several factors affect the minimum time acquisition frequency including integration time number of spectrometer channels samples averaged and computer speed If you specify that you want data to be stored every 100 milliseconds it is guaranteed that the delay will be at least 100 milliseconds but could be much longer depending on your experimental configuration OOlIBase32 spends a large amount of time calculating rendering and displaying the spectra in a spectral window You have the option to suspend graph display which greatly improves performance Enable Continue Until Manually Stopped to store data until you manually stop the acquisition process by clicking on the stop icon or selecting Time Acquisition Stop Enter an Initial Delay to set the delay before the time acquisition process begins However the delay countdown cannot begin until you initiate the time acquisition process by clicking on the start icon or selecting Time Acquisition Start from the menu Be sure to select Hours Minutes Seconds or Milliseconds immediately to the right of the initial delay entry Enter a value to set the Frequency of the data collected in a time acquisition process Data from a time acquisition is stamped with a time that is accurate to 1 millisecond Be sure to select Hours Minutes Seconds or Milliseco
60. aging boxcar smoothing distance from light source to sample etc you must store a new reference and dark spectrum A typical configuration for an irradiance experiment irradiance computer m Q 95 ean Experiment Tutorial Time Acquisition CO a F Time Acquisition Experiments Our OOIBase32 Spectrometer Operating Software allows you to perform time acquisition experiments Time acquisition experiments track processes perform kinetic analyses and monitor spectral events as a function of time You can collect as a function of time spectral data from up to 6 single wavelengths designated as Channels A through F and up to two mathematical combinations of these wavelengths designated as Combinations 1 and 2 You can acquire data in any mode For more details about this and other OOIBase32 functions refer to the OOlBase32 Spectrometer Operating Software Manual To perform a time series experiment in OOIBase32 follow these steps 1 While in scope mode store reference and dark spectra if desired and choose a view mode such as absorbance 2 Access the Time Acquisition Channel Configuration dialog box by selecting Time Acquisition Configure Configure Time ChannelE ChannelF Combination Combination2 Channels from the menu Channel A l Channel B Channel C Channel D Select Enabled to set the time acquisition Time Acquisition Channel Configuration calculation for the wavelength W
61. ake changes to these parameters instantly see their effect and save the data Most spectrometer system operating software does not allow such signal conditioning flexibility With OOIBase32 you can even perform time acquisition experiments for kinetics applications As part of the time acquisition function you can monitor and report up to six single wavelengths and up to two mathematical combinations of these wavelengths In addition you can perform reference monitoring with either a two channel spectrometer system or even a one channel system 00 OOIBase32 Spectrum1 olx File Edit View Overlay Spectrometer Spectrum Time Acquisition Window Help oem gt S X 2 2 m 2 Spectrum1 iof x Sie S ual m 2 SATII 4 nel BI kp Pb AI Integ Time fe TE Flash Delay fioo 4 52000 Strobe Correct for msec we 5 Mec Bf al See msec a Enable E Electrical Dark Now acquiring data for Spectrum Integration Time 18 msec Averages 4 Boxcar 0 OOIBase32 gives you complete control of setting the parameters for all system functions such as acquiring data designing the graph display using spectra overlays and configuring the cursor You can also save and then retrieve all of these system parameters for future experiments OOIBase32 has the benefit of giving you various software controlled triggering options for external events such as the firing of a laser or the pulsing of a light source 31 OOIBase32 Spectrometer Ope
62. alf length ISA bus card for single slot in desktop PC Resolution 12 bit Sampling frequency 1 MHz maximum F For the ADC1000 37 pin connector to A D card and 25 pin connector to Interface cable spectrometer Multiple channel capability up to 8 spectrometer channels for S2000 PC2000 spectrometers fnciallanion parameters set dip switch for Base Address default 768 decimal 300 hexadecimal P i set dip switch for IRQ default 7 Integration time 3 milliseconds to 60 seconds with S2000 series spectrometers 22 F ean A D Converters SAD500 P er SAD500 Serial Port Interface The SAD500 SERIAL PORT INTERFACE is a microprocessor controlled A D converter for serial port connection or stand alone operation The SAD500 can be used to interface to desktop or portable PCs PLCs and other devices that support the RS 232 communication protocol The following are directions for setting up your SAD500 Because A D converter installation goes hand in hand with software installation you will find directions for installing OOIBase32 Spectrometer Operating Software included in this section as well Interface the SAD500 to your PC Interfacing the SAD500 to a desktop or portable PC is simple 1 If you ordered your SAD500 mounted onto your spectrometer simply connect the 6 pin DIN end of the serial cable to the SAD500 and the DB9 end to your PC If you ordered your SAD500 in its own housing attach the 25 pin conductor ribbon cable from t
63. alog box the Base Address is aee 350 given in decimal followed by the hexadecimal equivalent in atl parenthesis For example 768 0x0300 is 768 decimal moate taraa Ecu Hacuss 300 hexadecimal 768 00300 x 7 4 If you are a Windows 95 98 user choose the same IRQ ila eas value selected in steps 8 and 9 on page 28 If you are a f g 115200 Fa Windows NT user choose the same IRQ value selected in AD500 Pixel Resolution step 3 on page 28 fj IV Compress SAD500 Data 5 For your setup only these parameters apply to your system Ignore the other settings they apply to other A D converters Click OK You can always change these settings once OOIBase32 is fully operational by selecting Spectrometer Configure A D Interface Cancel f5 If you do not see the Configure Hardware screen exit the software Then select Start Run and type C windows ooidrv ini for Windows 95 98 systems or c winnt ooidrv ini for Windows NT systems Notepad will open Edit this file for our device driver by finding the Initialized entry and making sure this line reads Initialized 0 Save the OOIDRV INI file and exit Notepad Restart OO Base32 You should now see the Configure Hardware dialog box 29 First in Photonics ean A D Converters DAQ 700 Oven Spectrometer Configuration Dialog Box Now that OOIBase32 is running you need to configure your system Select Spectrometer Configure from the me
64. an A D Converters ADC1000 and PC2000 Oer Spectrometer Configuration Dialog Box Now that OOIBase32 is running you need to configure your system Select Spectrometer Configure from the menu Go through each page in the Spectrometer Configuration dialog box to set system parameters See the OOIBase32 Spectrometer Operating Software Manual for details Inthe Wavelength Calibration page the coefficients for each spectrometer channel in your system have already been loaded as part of the spectrometer configuration file Check Spectrometer Configuration x Wavelength Calibration A D Interface Reference Monitoring Stray Light Correction Detector Linearity Spectrometer Type 52000 PC2000 AMD Ci ter T 5 the Enabled box for each spectrometer a T channel in your system aa eT 7 E Inthe A D Interface page enter the same Base Address 1 0 Range 800 040320 7 pag 0x0320 values as you did in the Configure Hardware elie 6S dialog box 784 0x0310 gt p H 800 0x0320 The Detector Linearity page in this dialog ore o aoo 832 0x0340 box allows you to enter coefficients for an algorthim that corrects for rare occurances of non linearity of the detector Contact Ocean Optics for more information Save the spectrometer configuration file by Cancel Apply Help choosing Spectrometer Save Configuration As from the OOIBase32 menu You can rename the file or use the default file name
65. aphy or capillary electrophoresis systems The cell is attached on line i e the light is projected through the sides of fused silica tubing without violating the tube integrity 54 F Sampling Chambers CUV UV CUV VIS O CUV UV CUV VIS Cuvette Holders The CUV UV and CUV VIS CUVETTE HOLDERS for 1 cm path length cuvettes couple via SMA terminated optical fibers to our spectrometers and light sources to create small footprint spectrophotometric systems for absorbance and transmission experiments These holders can be optimized for UV VIS NIR 200 1100 nm or VIS NIR 360 1100 nm applications Parts Included Cuvette holder assembly for holding 1 cm cuvettes Black cover for eliminating ambient light Two barbed fittings for connecting a temperature stabilizing water source Screwdriver for adjusting the fit of the cuvette Allen wrench for adjusting the collimating lenses eee i a Operation Attaching the Fibers 1 Attach one end of a SMA terminated optical fiber to one of the collimating lenses Attach the other end of this fiber the illumination fiber to a light source 2 Attach another SMA terminated optical fiber to the other collimating lens Attach the other end of this fiber the read fiber to the spectrometer Using the Fiber Supports 1 Snap the clamps around the fibers after the fibers are screwed into the light source and spectrometer 2 Lift the clamps until they support the fibers 3 Unsnap
66. arc xenon lamp for applications involving absorbance reflection fluorescence and phosphorescence measurements The PX 2 operates at speeds up to 220 Hz and offers critical pulse to pulse stability Parts Included PX 2 Pulsed Xenon Lamp 15 pin cable for connecting the PX 2 to the spectrometer 12V DC wall transformer for supplying power to the PX 2 Caution KA The beam emerging from the PX 2 produces ultraviolet radiation Direct contact with the beam could cause eye injury Never look directly into the light source Dangerous voltages present Never operate the PX 2 without its housing intact The SMA connector may get HOT during operation This instrument should not be used for any clinical or diagnostic purposes v V xe Handle with care Dropping the instrument may cause permanent damage Operation The lamp is triggered with TTL pulses delivered through the 15 pin connector located at the rear of the unit Our S2000 series spectrometers and software or any source of TTL signals supplies these pulses The lamp is powered by a 12V DC transformer or from a 12V battery if field use is desired Plug the wall transformer into a 110V outlet Plug the other end of the cord into the jack at the rear of the PX 2 Install the 15 pin cable into the rear of the PX 2 Connect the other end to the 15 pin connector on an S2000 Connect an optical fiber to the SMA terminated fiber optic port on the front panel Turn the power switch locate
67. arization resistant Fibers If you are using a UV light source the UV radiation degrades the silica in a standard patch cord over time resulting in increased absorption and invalid data The degradation is called solarization The active part of our solarization resistant fibers consists of a silica core surrounded by a silica cladding material Then the fiber is coated in aluminum which prevents the fiber from solarizing Optical Fiber Color Codes All Ocean Optics optical fibers are color coded for simple identification There are two color bands on one end of the fiber The color band nearest the termination identifies the fiber type i e its wavelength optimization and is either white UV VIS or black VIS NIR The second color band identifies the diameter of the fiber and is one of several different colors Use the table below as a handy reference Band Color Core Diameter um Band Color Fiber Type Purple x 8 VIS NIR only White UV VIS Bue D 50 Black P VIS NIR Green B 100 Yelow 200 Gray M 300 Red M 400 Brow B 600 The 300 um optical fiber is a solarization resistant Clear 1000 fiber for applications lt 250 nm Specifications Acrylate temperature range 40 to 100 C for 50 um optical fibers Jacket Nylon temp range 40 to 100 C for 100 200 400 600 and 1000 um optical fibers Aluminum temp range 269 C to 400 C for 300 um solarization resistant fibers Sheathing cabl
68. asurements Ocean Optics offers several variations on the Reflection Probe The RPH 1 REFLECTION PROBE HOLDER is an anodized aluminum platform with machined holes at 45 and 90 to hold our R200 Reflection as measuring the reflection properties of mirrors and anti reflection arts plastics food products etc The T300 RT UV VIS TRANSMISSION DIP PROBE couples to our T300 RT UV VIS spectrometers and light sources to create optical sensing systems for measuring transmission in chemical solutions and other liquids The standard T300 RT UV VIS has 2 300 um diameter solarization resistant fibers 1 illumination read 5 0 x 0 25 OD stainless steel ferrule and comes with removable tips in path lengths of 2 mm 5 mm or 10 mm The CC 3 COSINE CORRECTED IRRADIANCE PROBES are spectroradiometric sampling optics designed to collect radiation light from a 180 field of view thus eliminating light collection interface problems inherent to other sampling devices The CC 3 COSINE CORRECTOR 300 1000 nm has glass diffusing material The CC 3 UV COSINE CORRECTOR 200 nm to 2 um has Teflon diffusing material Both cosine correctors have a 0 25 O D barrel with a smooth yet rugged black oxide finish S The FL 400 FLAME RESISTANT FIBER PROBE is a heat resistant fiber optic probe that couples to Ocean Optics miniature fiber optic spectrometers to measure in situ emission spectra of samples such as dissolved metals and hi
69. be used for applications where more light reaches the spectrometer than likely can be digitized 74 90 UV successfully by the spectrometer s high sensitivity linear CCD array detector The FVA UV attenuates light uniformly at all wavelengths The WS 1 DIFFUSE REFLECTANCE STANDARD is a compact physical standard for use in performing reference measurements for diffuse reflectance applications especially color analysis The reflectance material in the WS 1 is Spectralon a substance that provides a nearly 100 diffuse reflective surface The ISP REF INTEGRATING SPHERE is an illuminated inte grating sphere that couples via optical fiber to our spectrometers to measure reflectance or emission The ISP REF has a transfer optic assembly for restricting the fiber viewing angle a 0 4 aperture sample port and a built in tungsten light source The FOIS 1 FIBER Optic INTEGRATING SPHERE was designed for emission experiments such as measuring the spectral properties of LEDs and other light sources The FOIS 1 consists of a 1 5 Spectralon sphere encased in an aluminum housing with a 0 375 input port that accepts light energy from 200 1100 nm and an SMA connector for coupling to the spectrometer The FOIS 1 also has threads for mounting the unit in a variety of configurations 74 F Sampling Optics 74 UV 74 VIS a 74 UV 74 VIS Collimating Lenses In order to obtain accurate data the light entering the sample and the light coll
70. card into the ISA bus slot and connect the necessary cables from the A D card to the spectrometer Make sure the connections are snug and restart your computer lf your A D converter is the DAQ 700 1 Install NI DAQ version 6 CD Driver Software the device driver library necessary for Windows 95 98 and NT systems to properly use the DAQ 700 on your computer 2 Insert the DAQ 700 into any available PCMCIA slot 3 Set the IRQ and Base Address values If you have Windows 95 98 select Start Settings Control Panel Double click the System icon Select the Device Manager tab Double click the hardware group named Data Acquisition Devices Double click DAQCard 700 Click the Resources tab Find the check box next to Use Automatic Settings Clear that check box deselect it Now change the settings for either or both the Input Output Range or the Interrupt Request To make this change double click either Input Output Range or Interrupt Request A dialog box giving the current hardware setting appears Use the two small arrows to the right side of the Value box to change the hardware interface parameters Choose values that say No devices are conflicting Click OK Click Yes at the Creating a Forced Configuration message box If you have Windows NT go to Start Programs Administrative Tools Common Windows NT Diagnostics Click on the Resources tab Select the IRQ button Find the IRQ that your computer assigned to the A D convert
71. ces DT 1000 ean PLCS inc Gently grip the top of the bulb and pull it away from the cylindrical unit Discard the bulb 5 Insert the new bulb into the top of the cylinder being careful to position the bulb s pins over the holes in the top of the cylinder fy The cylindrical unit is held in place with a small set screw By loosening it the user can slide the bulb unit up and down positioning it in front of the attenuator a metal disc that attenuates the light before going through the collimating lens Another set screw holds the attenuator in place 6 Put the DT 1000 casing back on and secure it with the 12 screws For directions on adjusting the focus of the collimating lens on the DT 1000 turn to Appendix B Specifications Spectral range 200 1100 nm Time to stabilized output 30 minutes Deuterium bulb lifetime 1 000 hours Tungsten halogen bulb lifetime 900 hours Power consumption 25 30 Watts peak to peak 0 05 maximum Stability drift of 0 5 hour Aperture 0 5 mm at lamp Connector SMA 905 Lamp voltage 85 volts DC nominal Operating lamp current 300 mA DC 1mA Inputs trigger inputs for lamp on off Outputs levels for lamp on off filament on off Power requirements 120 volts AC 0 50 A 50 60 Hz 220 volts AC 0 25 A 50 60 Hz 100 volts AC 0 60 A 50 60 Hz 240 volts AC 0 20 A 50 60 Hz 40 F Light
72. cing each other across the sample tubing and secured with the same F230 0 016 ID tubing sleeves and finger tight fittings Operation Eliminating Polyimide Jacketing 1 Prepare the silica sample tube by burning off the polyimide jacketing with a match or butane lighter 2 Make sure the tube has completely cooled and then rinse the tubing to remove any burn residue particles etc Inserting the Sample Tubing 1 Insert the sample tubing through a finger tight 10 32 fitting and tubing sleeve Carefully feed the tube through the through hole of the cross until the clear window is approximately at the center point of the cross 2 Tighten the 10 32 fitting until the tube is just snug enough to stay in place Do not over tighten 3 Install the other 10 32 fitting and sleeve on the tubing and into the cross Leave this fitting loose for now Configuring the Optical Fibers 1 The optical fiber may already be installed in the cross If it is not insert the fiber through a 10 32 fitting and tubing sleeve Insert the fiber into the through hole of the cross pushing it gently until it makes contact with the sampling tube Back the fiber off just enough to leave the sample tube free to manipulate Tighten the finger tight fitting to hold the fiber firmly Do the same for the other fiber as well 2 Connect one fiber to the SMA connector on a deuterium source we recommend the DT 1000 Al fiber for UV VIS work or the D 1000 for UV only 3
73. collimating lens 2 Attach the other end of this fiber the read fiber to the spectrometer Adjusting the Fit of the Cuvette The ISS 2 is preset for use with a 1 cm cuvette When properly adjusted the cuvette should fit snugly into the holder 1 Locate the two ball plunger screws 2 Use the screwdriver to loosen the two ball plunger screws until the ends are visible in the holder 3 Insert your cuvette into the holder 4 Tighten the screws until the ball contacts the cuvette and starts to compress Do not over tighten Installing Filters 1 Loosen the filter clamping screw with an Allen wrench 2 Insert the filter into the filter slot The filter slot can accommodate filters up to 6 mm thick 3 Clamp the filter in place by gently tightening with an Allen wrench Turning On the Lamp 1 Plug the wall transformer end into a 110 V outlet Plug the 12 V output end into the back of your ISS 2 2 Screw a fiber into the SMA connector 3 Find the on off switch on the back of the lamp and turn the lamp on Replacing the Bulb on the ISS 2 1 Order a replacement bulb 2 Turn off the lamp and allow it to cool 3 Remove the fan from the bottom of the lamp by loosening the four screws securing it to the base Two of the four screws also hold the two front legs in place Once the screws are loosened gently pull the fan along with the legs away from the lamp 4 Now that the fan is removed use an Allen wrench to loosen the set scr
74. d at the rear of the source to the ON position Select either MULTIPLE or SINGLE flash mode by moving the toggle on the rear of the source Configure OOIBase32 operating software to operate the PX 2 In the Acquisition Parameters dialog bar check the S2000 Strobe Enable box You can also enable this function by selecting Spectrum Configure Data Acquisition from the menu and then choosing the Strobe page of the dialog box 7 The flash should fire with a clearly audible ping DU e oea Application Tips Single Flash Mode Using the Single flash mode results in one flash per integration cycle Since the PX 2 has a maximum repetition rate of 220 Hz the minimum integration allowed in this mode is 5 milliseconds Multiple Flash Mode When using the Multiple flash mode the user needs to ensure that a constant number of flashes occurs for every integration cycle by setting the pulse rate and integration time This achieves a continuous and stable signal The pulse rate is determined by a jumper setting inside the S2000 JP3 The integration time is controlled via the 43 Light Sources PX 2 ean lt tics operating software To achieve a constant number of flashes per integration cycle the integration time must be a multiple of those shown in the following table 2000 JP3 For DAQ700 Integration For ADC500 and SAD500 Integration For ADC1000 Integration setting time must be a multiple of time m
75. diameter optical fibers 6 UV VIS and 6 VIS NIR illumination fibers plus 1 UV VIS fiber and 1 VIS NIR read fiber In addition the R200 MIXED has a 3 0 x 0 25 stainless steel ferrule to house its fiber bundles and couples easily to a dual channel spectrometer in which each channel is set for a different wavelength range Also available is the R200 REF which consists of an R200 7 Reflection Probe and an additional fiber optic to monitor an illumination source such as our LS 1 Tungsten Halogen Light Source The R200 REF is useful when a reflection experiment either does not allow the user to take frequent reference scans or includes an illumination source with an unstable spectral output One other option is the R200 ANGLE REFLECTION PROBE which has a bundle of seven 200 um fibers 6 illumination fibers around 1 read fiber and a 3 0 x 0 25 stainless steel ferrule with a 45 window This angled window removes the effects of specular reflection when the probe is immersed in dense liquids and powders Operation The reflection probe consists of a 6 fiber leg the illumination leg that should be coupled to the light source and a single fiber leg the read leg that attaches to the spectrometer 1 Inspect the ends of the fiber legs The hole in the SMA connector is noticeably larger in the illumination leg than the hole in the read leg Also the read leg should have colored bands 2 Attach the illumination leg to the light source
76. ding jacketing diameter 510 um Fiber bundle 1 single strand multi mode read fiber Wavelengths covered 200 750 nm Probe sleeve ferrule Nickel Probe sleeve ferrule length 8 0 or 20 cm Temperature range 269 C to 750 C Numerical aperture 0 22 Fiber termination SMA 905 88 ean PLCS inc F Optical Fiber Assemblies Ocean Optics offers an extensive line of optical fibers and accessories including patch cords bifurcated assemblies bushings and splitters for a variety of UV VIS and VIS NIR applications All optical fibers couple easily via SMA terminations to our miniature fiber optic spectrometers light sources and sampling optics Ocean Optics optical fibers offer great flexibility both in the literal sense by transporting light around corners for example and in the way fiber based systems are constructed by linking light sources and sampling optics for example to create an optical interface to the spectrometer Optical fibers allow the user to easily convert the optical interface from one setup to another absorbance reflectance and emission are the three basic options to create an almost endless variety of optical sensing systems These silica core and silica clad optical fibers are optimized for the UV VIS 200 750 nm or VIS NIR 450 1000 nm Standard assemblies are 2 meters in length and are available in sizes ranging from 8 um to 1000 um in diameter Custom options include optical f
77. down arrows range will be automatically selected or you may select a range using the up and down arrows Value fi 0 Value ov 20 013F 4 Conflict information Conflict information The setting you have chosen does not conflict with any The setting you have chosen does not conflict with any other devices other devices No devices are conflicting No devices are conflicting Cancel 8 While making this change notice the Conflict information area at the bottom Make sure you choose a value that says No devices are conflicting If it shows a conflict you must select a different value After selecting values with no conflicts click OK You will then see the Creating a Forced Configuration message box Click Yes 9 Note your values of both the Input Output Range Base Address and the Interrupt Request IRQ When you first run OOIBase32 you must enter these values in the Configure Hardware dialog box For Windows NT Users Find the IRQ and I O Range 1 Ifyou hear the happy sound go to Start Programs Administrative Tools Common Windows NT Diagnostics 2 In the Windows NT Diagnostics dialog box click on the Resources tab Select the IRQ button Find the IRQ that your computer assigned to the DAQ 700 Note this number 4 Select the I O Port button Find the I O Range Base Address that your computer assigned to the DAQ 700 Note this number This number is in hexadecimal
78. e Base Address and the Interrupt Request corresponds to the IRQ in our software By deselecting the Use automatic settings box in the previous step you disabled Plug and Play for the DAQ 700 But in order to fully disable Plug and Play you must also change the settings for either or both the Input Output Range or the Interrupt Request To make this change double click either Input Output Range or Interrupt Request A dialog box giving the current hardware setting appears On the right side of the Value box are two small arrows one up and one down You must use these arrows to change the hardware interface parameters of either the Input Output Range or the Interrupt Request 27 DAQCard 700 Properties x General NI DAQ Information Driver Resources y DAQCard 700 Resource settings Resource type Setting Toga Eaa 0120 013F Interrupt Request 10 Setting based on Basic configuration 0000 Change Setting ru Conflicting device list First in Photonics ean A D Converters DAQ 700 CO Edit Interrupt Request 21x Edit Input Output Range 12 xi Enter the interrupt request you would like to set for this device Enter the input output range you would like to set for this device You may either enter a specific value and the nearest valid value will be automatically selected or you may select a value You may either enter a specific range and the nearest valid using the up and
79. e DT 1000 DEUTERIUM TUNGSTEN HALOGEN LIGHT SOURCE combines a UV and VIS NIR light source in a single optical path It s range is from 200 1100 nm The LS 1 TUNGSTEN HALOGEN LIGHT SOURCE is a versatile source for the VIS Shortwave NIR 360 nm 2 um It offers high color temperature a long life and a built in filter slot for color correcting and other uses The PX 2 PULSED XENON LAMP 225 700 nm is a 220 Hz short are flashlamp It produces high energy pulses of brief duration and has two trigger modes The LS 450 BLUE LED PULSED LIGHT SOURCE is a compact low cost light emitting diode that produces pulsed or continuous spectral output at 470 nm to provide excitation for fluorescence measurements The R LS 450 is the rack mount version of the LS 450 and has many jumper control features The LS 1 CAL CALIBRATED LIGHT SOURCE is a NIST traceable calibrated light source for the VIS Shortwave NIR specifically LS 1 CAL calibrated for optical fibers The calibration data includes absolute intensities at the fiber entrance port between 300 1050 nm The HG 1 MERCURY ARGON e CALIBRATION SOURCE produces Mercury and Argon emission lines from 253 922 oi nm for use in per forming fast reliable spectrometer wavelength calibrations 33 Light Sources MINI D2T PtiCSme MINI D2T Miniature Deuterium Tungsten Light Source The MINI D2T MINIATURE DEUTERIUM TUNGSTEN LIGHT SOURCE combines the continuous spec
80. e a ADC500 SAD500 must ADC1000 must be a multiple of be a multiple of multiple of 16 512 128 64 14 128 32 16 112 32 8 4 10 8 with a min value of 24 ms 4 4 45 F Light Sources R LS 450 W R LS 450 Rack mount Blue LED Pulsed Light Source The R LS 450 BLUE LED PULSED LIGHT SOURCE is a compact low cost light emitting diode that produces pulsed or continuous spectral output at 470 nm the blue region for high sensitivity emission fluorescence measurements The R LS 450 is the rack mount version of the LS 450 The R LS 450 can be configured to operate in continuous wave mode through manual operation and through the software It can also be configured to operate in pulsed mode through manual operation and through the software R The R LS 450 is shipped with the following pins jumpered Jumper over pins in JP1 a jumper over the Manual pins in JP3 and a jumper over the 2 pins in JP2 for the fastest pulse rate available Operation with the R LS 450 Board You can configure the lamp s performance through a switch and three jumper blocks on the circuit board of the R LS 450 and if desired through one jumper block on the circuit board of the S2000 spectrometer The following lists the many different choices you have for configuring the R LS 450 for your application You need to determine the best mode of operation for your setup and configure your system appropriately S1 Switch The S1 Switch is a three position switc
81. e a jumper over Pins 3 4 la as LA lan Vo LA JP3 6 Set other acquisition parameters such as averaging and boxcar smoothing in the software via the Acquisition Dialog Bar or the Configure Data Acquisition dialog box To access the dialog box click the Data Acquisition icon or select Spectrum Configure Data Acquisition from the menu 7 Select Spectrum Configure Data Acquisition from the menu Choose the External Trigger page and select Hardware Trigger 8 To save processed data with each external trigger enable the Automatically save file on trigger box If you enable this function you will be presented with a file save dialog box with each trigger To avoid manually naming a file for each trigger you can enable the Autoincrement Filenames function by selecting File Autoincrement Filenames Enabled from the menu Choose a base name and starting index for the autoincremented files 9 Once you select an external trigger mode it will appear on your computer that your spectrometer is unresponsive Instead it is waiting for the trigger Activate your triggering device The acquisition parameters name of the window acquiring data and trigger mode are displayed in the main status bar It is important to note that if you apply triggers faster than the fixed integration time you will miss acquisitions 112
82. e back of your ISS 2 Screw a fiber into the SMA connector 3 Find the on off switch on the back of the lamp and turn the lamp on Replacing the Bulb on the ISS Order a replacement bulb Turn off the lamp and allow it to cool Use your Allen wrench to loosen the set screw on the bottom of the lamp You do not need to remove the set screw This screw holds the bulb in place 4 Locate the two set screws at the back of the lamp one above each back leg These two screws keep the two halves of the lamp together Remove the two screws Gently separate the two halves of the lamp and pull the bulb out of its housing Detach the wire and socket from the lamp leads Remove the old bulb unit and discard 7 Plug the new bulb into the socket and slide it forward into the front of the lamp as far as it will go oN a NM 62 Sampling Chambers ISS 90 N ean lt tics Tighten the set screw on the bottom of the lamp Close the two halves without pinching the wires Replace the two screws at the back of the lamp 10 Check the lamp output and adjust the focus of the SMA connector if necessary See Appendix B for instructions on adjusting the focus of your collimating lens Specifications Path length 1cm Collimating lens BK 7 glass 360 nm 2 um 5 mm diameter f 2 Collimating lens termination SMA 905 Filter slot accepts filters up to 1 4 6 mm in thickness Base material aluminum Spectral range 36
83. ean Optics HG 1 Mercury Argon lamp is ideal for this purpose If you do not have an HG 1 you will need a spectral line source that produces several at least 4 6 spectral lines in the wavelength region of your spectrometer Your spectrometer An optical fiber for spectrometers without a built in slit a 50 um fiber works best Either a spreadsheet program Excel or Quattro Pro for example or a calculator that performs third order linear regressions If you are using Microsoft Excel choose Tools Add Ins and check AnalysisToolPak and AnalysisToolPak VBA eee Calibrating the Wavelength of your Spectrometer 1 After placing OOIBase32 into Scope Mode take a spectrum of your light source Adjust the integration time or the A D conversion frequency until there are several peaks on the screen that are not off scale 2 Move the cursor to one of the peaks and carefully position it so that it is at the point of maximum intensity Record the pixel number that is displayed in the status bar located beneath the graph Repeat this step for all of the peaks in your spectrum 3 Using your spreadsheet create a table like the one shown on the next page In the first column place the exact or true wavelength of the spectral lines that you used Most calibration line sources come with a wavelength calibration sheet If you do not have a wavelength calibration sheet for your light source you can probably find the wavelengths for your spectral lin
84. earity page in this dialog box allows you to enter coefficients for an algorthim that corrects for rare occurances of non linearity of the detector Contact Ocean Optics for more information 24 F ean A D Converters SAD500 Oer Save the spectrometer configuration file by choosing Spectrometer Save Configuration As from the OOIBase32 menu You can rename the file or use the default file name your serial number spec You will then be asked if you would like to make this file the default spectrometer configuration file Choose Yes The next time you run OOIBase32 the software will use the file as the standard for your configuration When you exit OOIBase32 any changes to the configuration file will be automatically saved to the default file 00lBase32 Settings Dialog Box At this point it is a good idea to configure several OOIBase32 operation parameters Choose Edit Settings from the menu to open the OOIBase32 Settings dialog box Go through each page of this dialog box to select options for saving opening and printing data to configure default setting files and to select other important options such as storing and copying data and choosing warning messages See the OOIBase32 Spectrometer Operating Software Manual for details Configure Data Acquisition Dialog Box Finally select Spectrum Configure Data Acquisition from the menu to set your data acquisition parameters in the Configure Data Acquisition dialog box The Ba
85. ected after exiting the sample must be well collimated The 74 UV and 74 VIS COLLIMATING LENSES screw onto the end of SMA terminated optical fibers and other sampling optics to convert divergent beams of radiation light into a parallel beam Application Tips Using a collimating lens is easy Screw a collimating lens onto the end of any SMA terminated port to collect shape or focus light Collimating lenses are useful for any optical setup that requires the acceptance or transmission of parallel beams of light at the illumination source at the entrance optics or at both ends illumination and read of the setup That s important because the optical fibers Ocean Optics specifies for use with its spectrometers and light sources have a field of view FOV of 25 an acceptance angle that may not be appropriate for some experiments Collimating lenses are adjustable providing FOV angles from collimation near 0 to 45 Without the collimating lenses the light would disperse more than is required for efficient transmission and collection of the signal For directions on adjusting the focus of a collimating lens see Appendix B Specifications Lens diameter 5mm Lens length 10 mm f number f 2 74 UV material Dynasil 1100 quartz 200 nm 2 um 74 VIS material BK 7 glass 360 nm 2 um Lens barrel stainless steel with black oxide finish Threads UNC 3 8 24 Though the product can be used
86. eeeeeaaeeea 81 FOIS 1 Fiber Optic Integrating Sphere ccccccceceeceeeeeeeeeeeeeeeeaeeeeeneeeeaeeeseeeesteeeseenaeeeas 82 Table of Contents CO Fiber Optic Probes and ACCESSOPICS 0 0 02 cece cece ee teetee teste eeeteeeneeteeenes 83 R200 Bellecion Propels onesie ds dadeatadiduwess tgig cetesiadicadaedasiee 84 RPH 1 Reflection Probe Holden iscssivesiinsadec devices coeervuebccevevensadcettenesd A 85 T300 RT UV VIS Transmission Dip Probe c cccccceeeeeseeeeceeeeeeeeeeceeeeeseaeeeseeeesteaeeeeeeeeee 86 CC 3 Cosine corrected Irradiance Probes ccccccceceeeeceeeeeeseeeeeeeaeeeseeeeesaaeeseeeeeesnaaeenenes 87 FL 400 Flame resistant Fiber Probe eee ceeneeeeeeenneeeeeeeeaeeeeeeeeeaeeeeseeaaaeeeeesenaeeeeeeeaaaes 88 Optical Fiber ASSEMDIICS oe ec cece cece cee eeeeeseeeeeeeeeeeeeneeeneeneesneesneeneeeaeens 89 Experiment utorial osc ecdccsa se eeetceee rater sarereseteepetreecastereyeteteereenteoncueracteneeneon 91 AbDSOTbance EXPerime mts i ccsie satecocdiesofodicuts Sedlace T E 92 TRANSMISSION EXPEMIMONIS egcisccectvdusscecceduneResden sesnetwnp r seb Gecewey veh apne seeliavyy alee ceuvWe hd pawivis 93 RRETMSCHON EXPOrIMO Mts xc cece ase ece geadeseh coeticd ne cdengsdept dueasdas yuesgecd necetedaddicdeedaate cesaasaniaceauaniee 94 Relative IrradianGe Experiime mts assosiasie 95 Time Acquisition Experiments senesinin AE EEA 96 Appendix A Changing A D Converter Settings 00 0 0 eee 98 Base
87. eesnaaeeneaes 60 ISS Integrated Sampling System issiskiria inaita a aad 62 ISS 2 Integrated Sampling SyStem cccceeccceeceeeeeeeeeeeeeeeeeeeeeesaeeeeeeeeeseaeeeseeeeseieeeeeaeeee 64 ISS UV VIS Integrated Sampling SyStem c cecccecceeeeeeeeeeeeeeeeeaeeeeeeeeesaeeeseaeeeseeeeeeaeeee 66 FHS UV FHS VIS In line Filter Holders cccccccceseseseceeeeceeeceeceeccececececeeesauaeauaauanaeaeaeaeess 68 LPG Long Pass FOW Cells races cz sccesesassezetaede debeack vatehtesd aegea aaae s EEEE aaan 70 CUV CCE Electrophoresis Sample Cell cccceecsccececeeceeeeeeeeceeeeeaeeesecaeeesaeeseceeesnaeeneaes 72 Sampling CS isaac eee 74 74 UV 74 VIS Collimating LENS S 0 ccccccccccccesseeeeeeeesneeeeeeseaeeeeesseneeeeeesesaeeesensseeeeneees 75 74 90 UV Right Angle Reflector eee cccecececcceeeeeeeceeeeeeteeceeeeenseaceeeeeseeaeeeesneseaeeeeeseeaeeeneneees 76 74 OPM Optical Post MOUNT ceccccecceeeeeeeeeeeeeeeeeceeeeeeaaeeeeeeeeseaaeeeseeeesecaeeeseeeeeseieeeeenaeeseaes 77 74 ACH Adjustable Collimating Lens Holder c cccccccceeececeeeeeeeeeeeseeeeeeeaaeeseeeeessnaeeseees 78 FVA UV Fiber Optic Variable AttenUator c ccccceececeseeeeceeeeeeeeeeeaeeeeeeeeeseaeeeseaeeeteeeeeeeeeee 79 WS 1 Diffuse Reflectance Standard ccccccecesceeeeeeeeeeaeeeeeeeeeeeeaeeeeeeeeseaaeeseeneeeseaeeeeeneeeea 80 ISP REF Integrating Sphere ccceeccceceeceececeeeeeaeeeeeeeeeeaaeeeeneeeecaaeesseneeesaeesesaeeesea
88. egration time time between rising edges Trigger when using the External Synchronization Trigger mode 6 Channel 7 The analog input for spectrometer slave channel 7 7 Channel 6 The analog input for spectrometer slave channel 6 g D3 orEmemal Sowa A TE ate The Reet hanna Aa rigger ae to this line 9 Channel 1 The analog input for spectrometer slave channel 1 10 GND Ground supply voltage return or case ground 11 Channel 4 The analog input for spectrometer slave channel 4 12 Channel 5 The analog input for spectrometer slave channel 5 TTL inputs used to determine the triggering mode S1 SO Mode L X Normal or Continuous Scan L X External Software Trigger 13 SO and S1 H L External AO Trigger H H External Hardware Trigger X does not matter In the first 2 modes S0 is also used to enable disable light sources 14 Channel 3 The analog input for spectrometer slave channel 3 15 Channel 2 The analog input for spectrometer slave channel 2 106 Appendix D H1 Header Pins Analog Pin Description Analog Channel 0 Analog Channel 1 Analog Channel 2 Analog Channel 3 Analog Channel 4 Ground Reserved Analog Channel 7 CO CO N OD ol B C PM Analog Channel 6 oO Analog Channel 5 H1 and H2 Header Blocks connect a master unit to one or more slaves Continuous Strobe Rate ean lt tics H2 Header Pins Digital I 5 Description
89. ength 5 0 Terminations for illumination and read legs SMA 905 Path lengths 2 mm 5 mm and 10 mm stainless steel removable tips Temperature tolerance of epoxy to 400 C 86 F ean Fiber Optic Probes and Accessories CC 3 Per CC 3 Cosine corrected Irradiance Probes The CC 3 COSINE CORRECTED IRRADIANCE PROBES are spectroradiometric sampling optics designed to collect radiation light from a 180 field of view thus eliminating light collection interface problems inherent to other sampling devices The CC 3 COSINE CORRECTOR 300 1000 nm has glass diffusing material The CC 3 UV COSINE CORRECTOR 200 nm to 2 um has Teflon diffusing material The diffusing material is a thin disk that sits at the end of the barrel the Teflon material is 0 30 thick Both cosine correctors have a 0 25 OD barrel with a smooth yet rugged black oxide finish Operation Both cosine correctors have SMA 905 connectors for convenient coupling to optical fibers Screw the CC 3 or CC 3 UV onto the end of any SMA terminated optical fiber and the combination becomes a cosine corrected irradiance probe When coupled to a spectrometer these irradiance probes can be used to measure UV A and UV B solar radiation environmental light fields lamps and other emission sources CC 3 UV Cosine Corrector Function Test 3500 eum Teflon Disk 030 Cosine Function COUNTS 100
90. er Select the I O Port button Find the I O Range Base Address that your computer assigned to the DAQ 700 F CO Quick Start PtiCSme 4 Note these values When you first run OOIBase32 you must enter these values in the Configure Hardware dialog box If your A D converter is the SAD500 If your A D converter is the SAD500 and is mounted onto the spectrometer connect the cable from the SAD500 to your PC If you ordered your SAD500 in its own housing attach another cable from the spectrometer to the SAD500 Note the serial port number also called COM Port on the PC to which you are interfacing Plug the 12VDC wall transformer into an outlet and connect it to the SAD500 Step 2 Install OOlIBase32 Software Before installing OOIBase32 make sure that no other applications are running 1 Execute Setup exe At the Welcome dialog box click Next gt 2 At the Destination Location dialog box accept the default or choose Browse to pick a directory Click Next gt 3 At the Backup Replaced Files dialog box select either Yes or No We recommend selecting Yes If you select Yes you can choose Browse to pick a destination directory Click Next gt Select a Program Manager Group Click Next gt At the Start Installation dialog box click Next gt Follow all prompts regarding the Spectrometer Configuration diskette that came with your system At the Installation Complete dialog box choose Finish gt
91. er if the need arises to design and fabricate your own cabling system the following tables supply the necessary information J1 D 25 Interface Cable connects the S2000 master with the A D board J1 Pin Function A D Pin Connection 1 Analog Channel 0 37 2 Analog Channel 1 36 3 Analog Channel 2 35 4 Analog Channel 3 34 5 Analog Ground 19 6 Reserved 7 N C 8 N C 9 Digital Ground 7 10 A D Trigger 25 11 Master Clock 20 12 Digital Ground Not in Cable 13 5VDC 1 14 Analog Channel 4 33 15 Analog Channel 5 32 16 Analog Channel 6 31 17 Analog Channel 7 30 18 Analog Ground Not in Cable 19 N C 20 Continuous Strobe In 8 or use internal jumpers 21 External Software Trigger Out DO3 5 22 Spectrometer Mode Input S1 4 23 Integration Time Clock In 2 24 Strobe Enable Spectrometer Mode Input SO 23 25 Enable Read In 3 105 Appendix D ean lt tics J2 D SUB 15 Accessory Connector J2 Pin DB 15 Female Description 1 Single Strobe ae A e to pulse a strobe that is high at the start of each 2 Contin ous Strob a used to pulse a strobe that is divided down from Master 3 Voc The positive supply voltage 5VDC 4 External Hardware TTL trigger signal rising edge trigger input used in the External Hardware Trigger Trigger mode 5 External Synchronization TTL signal used to define the int
92. er modes of acquiring data are available Each mode involves connecting an external triggering device to the spectrometer and then applying an external trigger to the Dea of a e spectrometer before the software receives the data The length of the integration time and the source for the integration clock depend upon the mode chosen All other acquisition parameters are set in the software These directions on external triggering are for use with OOIBase32 H In order for you to use one of the External Triggering options it is imperative that you know the specifications and limitations of your triggering device The design of your triggering device may prevent you from using one of the external triggering modes as it is described in these pages For each external trigger mode Pin 3 of the J2 D SUB 15 Accessory Connector supplies voltage to the spectrometer To operate in the Software Mode the triggering device must be connected to Pin 8 of the J2 D SUB 15 Accessory Connector To operate in the Synchronization Mode the triggering device must be connected to Pin 5 of the J2 D SUB 15 Accessory Connector And to operate in the Hardware Mode the triggering device must be connected to Pin 4 of the J2 D SUB 15 Accessory Connector J2 D SUB 15 Accessory Connector female External Software Trigger In this level triggered mode the spectrometer is free running just as it is in the Normal Mode The spectrometer
93. er other than that in which it is published Trademarks Microsoft Excel Windows Windows 95 Windows 98 and Windows NT are either registered trademarks or trademarks of Microsoft Corporation DAQCard 700 and LabVIEW are registered trademarks of National Instruments GRAMS32 is a registered trademarks of Galactic Industries Corportation Spectralon is a registered trademark of Labsphere Inc Limit of Liability Every effort has been made to make this manual as complete and as accurate as possible but no warranty or fitness is implied The information provided is on an as is basis Ocean Optics Inc shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from the information contained in this manual ean PLCS inc F Table of Contents alice re i cy i1 o n EM AE nesters eye ree unetr ere ener 5 QUICK SIA cate secoreestspcin is E canes AE A 9 2000 Miniature Fiber Optic Spectrometer 0 00 0 eee 11 92000 SPeCIICAlONS fnticecsie osetia a S E eee eee needed 12 S2000 Board Layout saersi ia aaa a aaia adaini 13 A D SC OUNV CINE S sch scsi tices sect cients desatechpde oben seine tnta ena eeaPasldass 14 ADC500 ISA bus A D Convertehiccasasinedine ei nad a a a E 15 ADC1000 ISA bus A D Converter and PC2000 sssssssssssrssrrrssrrssrrssssssrnssrnnssrnsssrrssrns 19 SAD500 Serial Port IntertaCe 0i ii2 esol Ard ene aria etic i a EREE 23 DAQ 700 PCMCIA A
94. erence from the menu to permanently save the spectrum to disk Storing a reference spectrum is requisite before the software can calculate reflection spectra 3 While still in scope mode take a dark spectrum by first completely blocking the light path going to your sample If possible do not turn off the light source If you must turn off your light source to store a dark spectrum allow enough time for the lamp to warm up before continuing your experiment Take the dark reading by clicking the store dark spectrum icon on the toolbar or selecting Spectrum Store Dark from the menu This command merely stores a dark spectrum You must use the Save Dark command to permanently save the spectrum to disk Storing a dark spectrum is requisite before the software can calculate reflection spectra 4 Begin a reflection measurement by first making sure the sample is in place and nothing is blocking the light going to your sample Then choose the transmission mode icon on the toolbar or select Spectrum Transmission Mode from the menu The mathematics required to calculate reflection measurements are identical to those necessary to compute a transmission spectrum To save the spectrum click the save icon on the toolbar or select File Save Processed from the menu R If at any time any sampling variable changes including integration time averaging smoothing distance from light source to sample etc you must store a new reference and dark spectr
95. es Once you have selected your DAQCard 700 from the Device Manager click the Resources tab The entries here control the hardware interface to your DAQ 700 In this dialog box find the check box next to Use automatic settings Clear that check box deselect it System Properties 21x General Device Manager Hardware Profiles Performance View devices by type C View devices by connection Computer aS Data Acquisition Devices 9 Disk drives m Display adapters Floppy disk controllers Hard disk controllers F Keyboard EB Modem Monitor P Mouse Other devices m PCMCIA socket Ports COM amp LPT Sound video and game controllers ic m System devices Properties Refresh Remove Print Gl A E System Properties General Device Manager Hardware Profiles Performance View devices by type C View devices by connection Computer 8 CDROM H 9 Data Acquisition Devices H Disk drives El o Display adapters H Floppy disk controllers H Hard disk controllers H Keyboard E 8 Modem m Monitor H A Mouse Ej Ei Other devices H Q PCMCIA socket g Ports COM amp LPT F Sound video and game controllers eee EP es zl Properties Refresh Remove Print Cancel In the same dialog box you will see entries for Input Output Range and Interrupt Request The Input Output Range corresponds to th
96. es assuming that they are being produced by pure elements in a Chemistry or Physics textbook or handbook such as the CRC Handbook of Chemistry and Physics In the second column of this worksheet place the observed pixel number In the third column calculate the pixel number squared and in the fourth column calculate the pixel number cubed 103 Appendix C ee Independent Dependent Values computed from Variable Variables the regression output True Eo y Pixel Pixel Pixel Be Difference 253 65 175 30625 5359375 253 56 0 09 296 73 296 87616 25934336 296 72 0 01 302 15 312 97344 30371328 302 40 0 25 313 16 342 116964 40001688 313 02 0 13 334 15 402 161604 64964808 334 19 0 05 365 02 490 240100 117649000 365 05 0 04 404 66 604 364816 220348864 404 67 0 01 407 78 613 375769 230346397 407 78 0 00 435 84 694 481636 334255384 435 65 0 19 546 07 1022 1044484 1067462648 546 13 0 06 576 96 1116 1245456 1389928896 577 05 0 09 579 07 1122 1258884 1412467848 579 01 0 06 696 54 1491 2223081 3314613771 696 70 0 15 706 72 1523 2319529 3532642667 706 62 0 10 727 29 1590 2528100 4019679000 727 24 0 06 738 40 1627 2647129 4306878883 738 53 0 13 751 47 1669 2785561 4649101309 751 27 0 19 4 Now you are ready to calculate the wavelength calibration coefficients In your spreadsheet program find the functions to perform linear regressions if using Quattro Pro look under Tools Advanced Math if u
97. es for UV VIS NIR 200 1100 nm or VIS NIR 360 1100 nm applications Parts Included Cuvette Holder assembly for holding 10 cm cuvettes Black Cover for eliminating ambient light Two Barbed Fittings for connecting a temperature stabilizing water source Allen wrench for adjusting collimating lenses eee d Operation Attaching the Fibers 1 Attach one end of a SMA terminated optical fiber to one of the collimating lenses Attach the other end of this fiber the illumination fiber to a light source 2 Attach another SMA terminated optical fiber to the second collimating lens Attach the other end of this fiber the read fiber to the spectrometer Using the Fiber Supports 1 Snap the clamps around the fibers after the fibers are screwed into the light source and spectrometer 2 Lift the clamps until they support the fibers To remove the fibers simply unsnap the clamps Installing Filters 1 Tighten the thumb wheel completely when not using a filter 2 Loosen the thumb wheel on the left side of the cuvette holder creating enough space for the filter 3 Insert the filter into the filter slot which can hold filters up to 6 mm thick and tighten the thumb wheel Using the Temperature Stabilization Feature This feature is used to heat or cool the cuvette holder base and cuvette 1 Remove the two plugs from the top side of the base The plug on the right side of the base should stay in place but may require thread ta
98. es intense continuous spectral output from 200 400 nm making it especially useful for UV spectroscopy The D 1000 has a highly stabilized microprocessor based power supply designed for optimum stability with a maximum fluctuation of just 0 05 peak to peak and drift of 0 5 hr The lamp can be controlled from the switches on the front panel or through digital lines Parts Included D 1000 Deuterium Light Source Safety eyewear for protection against dangerous ultraviolet radiation Power cord for connecting D 1000 to outlet Allen wrench for adjusting the focus of the collimating lens Caution kA The beam emerging from the D 1000 produces visible light and invisible ultraviolet radiation Direct contact with the beam could cause serious eye injury Safety eyewear must be worn at all times while operating the D 1000 Do not remove any safety device installed Dangerous voltages present Only qualified service personnel should service the D 1000 This instrument should not be used for any clinical or diagnostic purposes Handle with care Dropping the D 1000 may cause permanent damage BET ye For optimum performance below 250 nm use a solarization resistant fiber with this lamp Operation Allow 30 minutes for the lamp to warm up and for the power to stabilize The spectral output as delivered by an optical fiber will decrease significantly at wavelengths lower than 250 nm due to attenuation in the lamp envelope the decrease in
99. ew that is underneath the fan This screw holds the bulb in place You do not need to remove the set screw loosening it is sufficient 5 Locate the two set screws at the back of the lamp one above each back leg These two screws keep the two halves of the lamp together Remove the two screws 64 Sampling Chambers ISS 2 i SOS SON ean ptics Inc Gently separate the two halves of the lamp and pull the bulb out of its housing Detach the wire and socket from the lamp leads Remove bulb unit and discard Plug the new bulb into the socket and slide it forward into the front of the lamp as far as it will go Tighten the set screw on the bottom of the lamp to hold the bulb in place Close the two halves of the lamp being careful not to pinch the wires Replace the two screws at the back of the lamp and secure the fan to the bottom of the lamp Specifications Path length 1 cm Collimating lens BK 7 glass 360 nm 2 um 5 mm diameter f 2 Collimating lens termination SMA 905 Filter slot accepts filters up to 1 4 6 mm in thickness Base material aluminum Spectral range 360 nm 2 um 9 0 cm x 5 0 cm x 3 2 cm LWH Dimensions 3 5 x 2 0 x 1 25 LWH Power input 12 VDC 800 mA 7 20 VDC 0 5 2 amps Power output 6 5 watts Bulb life 900 hours Bulb color temperature 3100K Output to bulb 5 volts 1 3 amps Output regulation 0 2 voltage
100. f a read fiber to the second port on the LPC and the other end to your spectrometer 70 ean Sampling Chambers LPC Orr Assessing if the LPC is Free of Particles Fluids need to be relatively particle free Particles larger than 20 um can be trapped inside the tubing and can then block or scatter a significant amount of light To rid the LPC of particles follow these steps 1 Pump the sample fluid through the LPC 2 While in Scope Mode save a dark spectrum with the light source off and a reference spectrum with the light source on 3 Continue to pump the sample fluid and switch to the Absorbance Mode Ideally you should see a spectrally flat line Particle effects manifest themselves as an exponentially decreasing curve from shorter to longer wavelengths The length of time that you pump the sample and the magnitude of the absorbance peak depends upon the time required and the minimum detectable absorbance value for your specific analysis Pre filtering of the sample may be required to eliminate this exponentially decreasing absorbance spectrum if it is significant to your analysis Specifications 1 meter 5 meter and 10 meter options standard custom Path lengths lengths also available Teflon Amorphous Fluoropolymer 2400 Tubing 560 um inner diameter 800 um outer diameter Refractive index 1 29 Internal volume 250 ul meter tubing can be altered by perfluorinated solvents FREON 113
101. gh temperature plasmas The FL 400 is a high temperature 400 um gold jacketed UV VIS optical fiber in an 8 long nickel sleeve It can operate in environments up to 750 C The probe accepts a standard wire loop for emission measurements of dissolved metals 83 Probes or other 0 25 O D probes during reflection measurements such coatings and measuring the visual properties of color in paints graphic ean PLCS inc F Fiber Optic Probes and Accessories R200 R200 Reflection Probes R200 REFLECTION PROBES couple to Ocean Optics miniature fiber optic spectrometers and light sources to create small footprint optical sensing systems for fluorescence and reflection measurements Ocean Optics offers several variations on the Reflection Probe The R200 7 REFLECTION PROBE consists of a bundle of 7 optical fibers 6 illumination fibers around read fiber each of which is 200 um in diameter A 3 0 x 0 25 stainless steel ferrule houses the fiber bundle Other options of this standard probe assembly include the R400 7 which has a bundle of optical fibers 400 um in diameter and the RP200 7 which has a 3 0 x 0 25 plastic ferrule to house the fiber bundle The RP200 7 is useful where a stainless steel ferrule may not be suitable such as some applications involving corrosive samples For reflection experiments across the UV VIS Shortwave NIR 200 1100 nm there is the R200 MIXED The R200 MIXED consists of fourteen 200 um
102. h on the R LS 450 The switch can be positioned in continuous wave operation no operation and pulsed operation Jumper Block 1 JP1 There is only one set of pins in JP1 If other jumper blocks are configured correctly a jumper over JP1 allows you to turn the R LS 450 on and off via the Strobe Enable feature in OOIBase32 software and to even control the pulse rate through the Flash Delay feature in OOIBase32 software This feature is only available with a J or later version of the S2000 To find out if you have a J series or later S2000 see the third letter in your S2000 serial number Jumper Block 2 JP2 There are nine sets of pins in JP2 The number of pulses per second of the R LS 450 depends on the pins you jumper on JP2 However the pulses per second are also dependent upon the master frequency of your A D converter A jumper over the CW pins makes the R LS 450 continuously on which means that there is no pulsing of the light source Other jumper blocks must be configured correctly A jumper over the 2 2 2 2 2 2 and 2 pins controls the pulse rate per second of the R LS 450 depending on the A D converter you are using to interface to your S2000 See table below for pulse rates A jumper over the CS pins allows you to control the pulse rate via the OOIBase32 software See Using JP3 on the S2000 on the next page for more information Pins on the JP2 Function Fisquoncy Ha F
103. h the other end of this fiber to a UV light source Attach another SMA terminated optical fiber to the collimating lens directly opposite of the first fiber It too has a red lens cover Attach the other end of this fiber to the spectrometer 2 For VIS measurements attach one end of a SMA terminated optical fiber to one of the collimating lenses with the VIS label and a black lens cover Attach the other end of this fiber to a light source Attach another SMA terminated optical fiber to the collimating lens directly opposite of the first fiber It too will have a black lens cover Attach the other end of this fiber to the spectrometer Attaching the Fibers for Fluorescence Measurements 1 Attach one end of a SMA terminated optical fiber to one of the collimating lenses Attach the other end of this fiber to a light or excitation source Typically for fluorescence measurements the illumination fiber connects to a UV lens 2 Attach another SMA terminated optical fiber to the collimating lens to the immediate left or right of the first fiber The two collimators must be positioned at 90 for fluorescence measurements Attach the other end of this fiber to the spectrometer Typically for fluorescence measurements the read fiber connects to a VIS lens Using the Fiber Supports 1 Snap the clamps around the fibers after the fibers are screwed into the light source and spectrometer 2 Lift the clamps until they support the fibers 3 Unsnap the c
104. hat s small enough to fit into the palm of your hand The 2000 is a high sensitivity low cost UV VIS Shortwave NIR spectrometer for low light level applications that demand high detector sensitivity The S2000 accepts light energy transmitted through optical fiber and disperses it via a fixed grating across the detector which is responsive from 200 1100 um Up to seven spectrometer channels can be added to expand wavelength range perform multiple tasks or provide reference monitoring The master and slave channels are all accessed through a single program for near synchronous operation In addition we offer over 200 spectrophotometric accessories that help to create fully integrated optical sensing systems The Modular Approach A typical Ocean Optics small footprint system comprises five basic elements the S2000 Miniature Fiber Optic Spectrometer an A D converter our operating software a light or excitation source and sampling optics The light or excitation source sends light through an optical fiber to the sample The light interacts with the sample Then the light is collected and transmitted through another optical fiber to the spectrometer The spectrometer measures the amount of light and the A D converter transforms the analog data collected by the spectrometer into digital information that is passed to the software providing the user with application specific information We offer several of our own A D converters for interfacing the spec
105. he FOIS 1 to the point where it interferes with the SMA terminated output port you will not collect radiation from a 180 field of view 4 Use the 2 mounting holes for mounting the FOIS 1 to other components There is a 1 4 20 threaded hole and an 8 32 threaded hole FOIS 1 mounted onto one arm of the 74 ACH Specifications Spectral range 200 1100 nm Dimensions 2 25 x 2 25 x 2 125 LWH Sample port aperture 0 375 Sphere coating Spectralon 2 6 32 threaded holes Top cap mounts 2 8 32 threaded holes 1 1 4 20 threaded hole in center Side mounts SMA connector for coupling optical fiber to the spectrometer 8 32 threaded holefor 74 OPM Optical Post Mount Connector SMA 905 82 ean IPLICS inc y First in Photonics Fiber Optic Probes and Accessories Ocean Optics fiber optic probes couple to our miniature fiber optic spectrometers and light sources to create a variety of optical sensing systems Each probe consists of silica core silica clad optical fiber 0 22 NA and a sampling optic and are available for UV VIS high OH content or VIS NIR low OH content applications All standard fiber optic probes are 2 meters in length and have SMA terminations Custom probe assemblies are also available R200 REFLECTION PROBES couple to Ocean Optics miniature fiber optic spectrometers and light sources to create small footprint optical sensing systems for fluorescence and reflection me
106. he spectrometer to the SAD500 Then connect the 6 pin DIN end of the serial cable to the SAD500 and the DB9 end to your PC 2 For either configuration note the serial port number also called COM Port on the PC to which you are interfacing Older PCs may not have numbered ports 3 Plug the 12VDC wall transformer into an outlet and connect it to the SAD500 Install OOIBase32 Before installing OOIBase32 make sure that no other applications are running 1 Execute Setup exe At the Welcome dialog box click Next gt At the Destination Location dialog box accept the default or choose Browse to pick a directory Click Next gt At the Backup Replaced Files dialog box select either Yes or No We recommend choosing Yes If you select Yes you can choose Browse to pick a destination directory Click Next gt Select a Program Manager Group Click Next gt At the Start Installation dialog box click Next gt Follow all prompts regarding the Spectrometer Configuration diskette that came with your system At the Installation Complete dialog box choose Finish gt When prompted to do so restart your computer when the installation is complete PU D La M Run OOIBase32 After you restart your computer navigate to the OOIBase32 icon and select it The first time you run OOIBase32 after installation you must follow several prompts before you can begin taking measurements Operator and Serial Number Dial
107. hile still in Scope Mode take a dark spectrum by first disconnecting the optical fiber from the lamp Take the dark reading by clicking the store dark spectrum icon on the toolbar or selecting Spectrum Store Dark from the menu 3 Fill the tube with the blank solution or solvent The peak intensity of the reference signal should be about 3500 counts Take a reference spectrum by first making sure nothing is blocking the light path going to your sample Take the reference reading by clicking the store reference spectrum icon on the toolbar or selecting Spectrum Store Reference from the menu 4 Reconnect the optical fiber to the lamp 5 Switch from Scope Mode to Absorbance Mode 6 The data can viewed as a time series of values from a single wavelength an integrated band around a wavelength or a mathematical combination of wavelengths Consult the directions in the OOIBase32 Spectrometer Operating Software Manual for using the time series functions Specifications Dimensions 1 09 x 1 09 Maximum fused silica tubing allowed 500 um in diameter ID tubing sleeves F230 0 016 Cell fixture 10 32 PEEK Cross Upchurch P 729 Through hole size 0 020 Coned female threads 10 32 Fingertight fittings F 300 PEEK UV waveguide optical fibers Al jacketed 300 um diameter solarization resistant silica core silica clad Path length silica tubing diameter Size of light beam reaching sample 5 mm circular
108. holder and tungsten halogen light source It couples to our spectrometers with optical fiber to create a small footprint systems for VIS NIR 360 1100 nm The ISS has a 10 000 hour bulb and the fan is enclosed in the base Parts Included ISS cuvette holder and light source assembly 12VDC wall transformer for providing power l cm square plastic cuvette for holding samples Screwdriver for adjusting the fit of the cuvette Allen wrench for adjusting the collimating lens eee eo a Operation Attaching the Fibers 1 Attach a SMA terminated optical fiber to the collimating lens 2 Attach the other end of this fiber the read fiber to the spectrometer Adjusting the Fit of the Cuvette The ISS is preset for use with a 1 cm cuvette When properly adjusted the cuvette should fit snugly into the holder 1 Locate the two ball plunger screws 2 Use the screwdriver to loosen the two ball plunger screws until the ends are visible in the holder 3 Insert your cuvette into the holder 4 Tighten the screws until the ball contacts the cuvette and starts to compress Do not over tighten Installing Filters 1 Loosen the filter clamping screw with an Allen wrench 2 Insert the filter into the filter slot The filter slot can accommodate filters up to 6 mm thick 3 Clamp the filter in place by gently tightening with an Allen wrench Turning On the Lamp 1 Plug the wall transformer end into a 110 V outlet Plug the 12 V output end into th
109. ialog box click on the Resources tab Select the IRQ button Find an available IRQ a number unassigned to a device Select the I O Port button Find an available I O Range Base Address a number or range of numbers unassigned to a device The number is in hexadecimal Note these available settings When you first run OOIBase32 you must enter these values in the Configure Hardware dialog box l Windows NT Diagnostics DAVENT SBE l Windows NT Diagnostics WDAVENT I x Eie Help File Help Version System Display Drives Memoy Version System Display Drives Memory Services Resources Environment Network Services Resources Environment Network Include HAL resources I Include HAL resources I IRQ _ Device Bus _ Type Address 0 i8042prt 0 Isa 0060 0060 i8042prt 0 Isa 03 Serial 0 Isa 0064 0064 i8042prt 0 Isa 04 Serial 0 Isa 0170 0177 atapi 0 Isa 06 Floppy 0 Isa OICE O1CF YgaSave 0 Pci 10 El90x 0 Pci O1FO 01F7 atapi 0 Isa 12 i8042prt 0 Isa O2F8 02FE Serial 0 Isa 14 atapi 0 Isa 0376 0376 atapi 0 Isa 15 atapi 0 Isa 0378 037A Parport 0 Isa 03B0 03BB VgaSave 0 Pci 03C0 03CF STBInc 0 Pci 03C0 03DF WVgaSave 0 Pci 03D4 03DB STBInc 0 Pci 03F0 03F5 Floppy 0 Isa OSF6 O3F6 atapi 0 Isa 03F7 03F7 _Floooy oo ia Z IRG 120 Port DMA Memory Devices IRQ i10 Pot DMA Memory Devices Properties Refresh Print Properties Refresh Print With Windows NT devices cannot sha
110. ibers with solarization resistance properties for applications lt 250 nm and in lengths other than 2 meters Caution Gently remove the plastic cover from the SMA connector before use Pulling the SMA connector away from the fiber when removing the plastic cover will permanently damage the fiber When fibers break they stop transmitting light Be sure to inspect fibers by eye to determine if light is being transmitted Do not coil the fiber too tightly The maximum sustained bend radius of a 400 um fiber is 10 cm Bending the fiber will cause attenuation To minimize this effect add extra strain relief to both ends of the fiber Do not exceed the temperature specifications for the materials involved 200 C for the fiber 100 C for PVC cabling 100 C for standard epoxy Do not allow the fiber to be bent at a sharp angle A bending radius of less than one inch is dangerous Keep connectors and probe tips covered when the fibers are not being used Clean ends of the fibers with lens paper and distilled water alcohol or acetone Avoid scratching the surface verve V amp amp F Do not immerse fiber ends in caustic materials or other solutions that can damage quartz or aluminum Operation Patch Cords Patch cords are single strands of optical fiber The active part consists of a silica core surrounded by a silica cladding material The fiber is very fragile and if not protected by a suitable buffer material would be nearly u
111. ibration numbers when calibrating the spectral response of your system with the lamp and a CC 3 cosine corrector and fiber its name contains the lamp s serial number followed by CC3 LMP Copy these two files into your OOIIrrad Irradiance Software directory Start OOlIrrad Irradiance Software Make sure you are in Scope Mode Under the Lamp menu options choose Select Lamp A window opens in which you must choose the Lamp Calibration Report file that reflects your optical setup Under the Spectrometer menu options choose Configure Fibers Enter the fiber diameter values for each channel in your setup If using a bare fiber enter the fiber s diameter here If using a fiber with a CC 3 cosine corrector enter 3 900 Whatever optical setup you wish to use for your application you must also use for calibrating the spectral response of your system For example if you are going to use a 200 um fiber with a CC 3 cosine corrector for your application you must use the same 200 um fiber and CC 3 for calibrating the spectral response of your system To the right of the displayed spectrum enter the data acquisition parameters for your setup The values for Scans to Average and Smoothing Size must be the same for your reference dark and irradiance scans You may use a different integration period for your reference scan but you must use the same integration period for your dark and irradiance scans Under Spectrometer menu options choose Calibrate and
112. ided by adjusting through software the spectrometer integration time to limit the interval during which the detector collects light somewhat akin to changing the shutter speed on a camera to a faster exposure time Other options include using different gratings changing the optical bench entrance aperture by installing slits or using small diameter optical fibers or adding neutral density filters to the optical path The FVA UV fits in where these intensity reduction techniques are either unworkable or undesirable Operation The attenuator comes with two collimating lenses one screwed in at each side The thumb wheel position is held with the set screw A 6 32 white nylon screw pinches the mechanical adjustment wheel Loosening the white nylon screw allows the mechanical adjustment wheel to be turned When the wheel is positioned to your liking tighten the white nylon screw to keep the wheel in place during your measurement The wheel is placed between two Teflon washers to provide smooth operation and is knurled along the edge for fingertip gripping To completely close the attenuator turn the wheel to the white line marking and tighten the white nylon screw Using the Adapters With the FVA ADP UV and FVA ADP VIS users can connect the attenuator directly to any light source having a collimating lens at its aperture To use these adapters follow these steps 1 Use an Allen wrench to loosen the set screw of one of the collimating
113. ight of the second black wire is the letter C In order to remove the bulb you will need to unscrew the nut holding the collimating lens and SMA connector in place It is positioned in such a way that if the nut remains in place you will not be able to take out the deuterium bulb Use a 3 8 wrench to remove the nut Use a Phillips head screwdriver to remove the two screws securing the bulb to the black platform Remove the old bulb unit Inspect the new bulb unit but avoid touching the glass casing or envelope around the bulb as the oils from your skin will deteriorate the bulb Inside the envelope is a triangle shaped filament The filament has a square opening The light passes through the square opening to the collimating lens Take the new bulb carefully feeding the three wires through the hole in the black platform and position it so that the square opening in the filament faces the collimating lens Screw in the two screws that holds the bulb to the platform and the nut that holds the collimating lens in place Secure the three wires to the green electronic board Attach the red wire to the top screw labeled A Attach the black wires to the screws on the board labeled H and C It does not matter which black wire is attached to screw H or C Put the D 1000 cover back on and secure it with the 12 screws For directions on adjusting the focus of the collimating lens on the D 1000 turn to Appendix B
114. in nm FWHM DISPERSION nm pixel Spectral range of the grating 2048 number of pixels or detector elements RESOLUTION in pixels typical values from slit size or fiber diameter see below 5 um slit 3 0 pixels 50 um slit 6 5 pixels 10 um slit 3 2 pixels 100 um slit 12 0 pixels 25 um slit 4 2 pixels 200 um slit 24 0 pixels OPTICAL RESOLUTION in nm DISPERSION X RESOLUTION 11 2000 Miniature Fiber Optic Spectrometers 2000 Specifications Absolute Maximum Ratings Vcc Voltage on any pin Physical Specifications Physical dimensions no enclosure Physical dimension with enclosure Weight Power Power requirement master Power requirement slave Supply voltage Power up time Spectrometer Design Focal length input Focal length output Input fiber connector Gratings Entrance slit Detector ilters Spectroscopic Integration time ynamic range Signal to Noise Readout noise single dark spectrum Resolution FWHM Stray light Spectrometer channels Environmental Conditions Temperature F d D 2x 10 lt i cean PtiCS me l 5 5 VDC Vcc 0 2 VDC 5 47 x 3 90 x 0 75 LWH master only 139 mm x 99 mm x 19 mm LWH master only 5 63 x 4 09 x 1 58 LWH master only 143 mm x 104 mm x 40 mm LWH master only 200 g master only no enclosure 130 mA at 5 VDC 70 mA at 5 VDC 4 5 5 5 V 3 msec asymmetric crossed Czerny Turner 42 mm 68
115. ing PVC with Kevlar reinforcement standard PVC Monocoil optional BX flexible metal sleeve optional Connector termination SMA 905 Fiber core pure fused silica Cladding doped fused silica Fiber profile step index multi mode Operating wavelengths UV VIS 200 750 nm VIS NIR 450 1000 nm Numerical aperture 0 22 Recommended minimum bend radius momentary 200x the fiber radius for standard patch cords long term 400x the fiber radius for standard patch cords 90 ean PLICS inc First in Photonics Experiment Tutorial Before you begin your experiment double check that you have correctly installed your A D converter installed the operating software and set up your light source and other sampling optics Next 1 Open OOIBase32 Although you already configured your hardware when you installed your A D converter double check that A D Interface settings correspond to your setup by choosing Spectrometer Configure from the menu 2 Now check your spectrometer setup configurations in OOIBase32 A Wavelength Calibration Data Sheet and a floppy diskette with the Spectrometer Configuration file on it is shipped with each spectrometer You will need these calibration coefficients on this sheet Select Spectrometer Configure from the menu and choose the Wavelength Calibration page For each spectrometer channel in your system enable the channel and make
116. ings once OOIBase32 is fully operational by selecting Spectrometer Configure A D Interface Cancel f If you do not see the Configure Hardware screen exit the software Then select Start Run and type C windows ooidrv ini for Windows 95 98 systems or c winnt ooidrv ini for Windows NT systems Notepad will open Edit this file for our device driver by finding the Initialized entry and making sure this line reads Initialized 0 Save the OOIDRV INI file and exit Notepad Restart OO Base32 You should now see the Configure Hardware dialog box 17 First in Photonics ean A D Converters ADC500 Oven Spectrometer Configuration Dialog Box Now that OOIBase32 is running you need to configure your system Select Spectrometer Configure from the menu Go through each page in the Spectrometer Configuration dialog box to set system parameters See the OOIBase32 Spectrometer Operating Software Manual for details Inthe Wavelength Calibration page the coefficients for each spectrometer channel in your system have already been loaded as part of the spectrometer configuration file Check Spectrometer Configuration x Wavelength Calibration A D Interface Reference Monitoring Stray Light Correction Detector Linearity Spectrometer Type 51000 PC1000 the Enabled box for each spectrometer A D Converter Type apc500 PC1000 7 channel in your system Interupt Request RQ 5 gt Inthe A D
117. ion source for UV VIS Shortwave NIR spectrophotometric systems The HG 1 produces Mercury and Argon lines from 253 922 nm for use in performing fast accurate spectrometer wavelength calibrations The HG 1 has an SMA 905 termination for connecting to optical fibers Caution KA The beam emerging from the HG 1 produces ultraviolet radiation Direct contact with the beam could cause serious eye injury Never look directly into the light source KA Never take apart the HG 1 The HG 1 contains mercury Dangerous voltages present No user serviceable parts inside Fa The SMA connector may get HOT during operation Setting Up To re calibrate the wavelength of your spectrometer you will need the following The HG 1 Mercury Argon lamp Your spectrometer An optical fiber for spectrometers without a built in slit a 50 um fiber works best Either a spreadsheet program Excel or Quattro Pro for example or a calculator that performs third order linear regressions If you are using Microsoft Excel choose Tools Add Ins and check AnalysisToolPak and AnalysisToolPak VBA eee Operation 1 Plug the wall transformer end of the HG 1 s power supply into a standard 110 V outlet Plug the 12 V output end into the back of your HG 1 Or insert a 9V battery not included 2 Attach a fiber from the spectrometer into the SMA connector on your HG 1 If your spectrometer does not have an entrance slit use a 50 um diameter or smaller optical fibe
118. is edge triggered mode the spectrometer is idle until you apply the initial trigger to the spectrometer With each trigger the following occurs 1 the spectrometer stops taking its current scan and data is transferred to the software 2 the spectrometer begins a new scan 3 the spectrometer integrates until another trigger is applied In the Synchronization Mode the integration time is set by the frequency of triggers applied to the spectrometer The effective integration time is the time between rising edges of signals applied to Pin 5 of the J2 Accessory Connector All other acquisition parameters are set in the software The source for the integration clock is the external triggering device itself such as a periodic TTL signal must synchronize your scans to an external clock source are using a lock in amplifier are using a chopper To Use the Synchronization Mode 1 Supply a line from your triggering device to Pin 3 of the J2 Accessory Connector to provide the positive voltage 5 VDC to the spectrometer See figure on page 109 for pin location We do not advise using an outside source to supply the voltage as it is based on a referenced ground and your reference may be different from ours Using Pin 3 to supply voltage ensures that the spectrometer will receive the appropriate voltage for the trigger event 2 Supply a line from Pin 5 of the J2 Accessory Connector to your triggering device See figure on page 109 for pin locati
119. is fully seated in the motherboard before screwing the tab on the A D card to the computer Do not bend the card or move it from side to side once it is seated in the slot For the ADC1000 attach the D37 end of the cable to the card and the D25 end to the spectrometer For the PC2000 attach the desired optical fiber to the SMA connector on the PC2000 Reinstall the cover Install OOIBase32 Before installing OOIBase32 make sure that no other applications are running bo RO rAINN Execute Setup exe At the Welcome dialog box click Next gt At the Destination Location dialog box accept the default or choose Browse to pick a directory Click Next gt At the Backup Replaced Files dialog box select either Yes or No We recommend selecting Yes If you select Yes accept the default or choose Browse to pick a destination directory Click Next gt Select a Program Manager Group Click Next gt At the Start Installation dialog box click Next gt Follow all prompts regarding the Spectrometer Configuration diskette that came with your system At the Installation Complete dialog box choose Finish gt When prompted to do so restart your computer when the installation is complete 20 F ean A D Converters ADC1000 and PC2000 e Run OOIBase32 After you restart your computer navigate to the OOIBase32 icon and select it The first time you run OOIBase32 after installation you must follow several p
120. ite UV Off button located on the front panel Then flip the power switch at the rear of the D 1000 just above where the power cord connects to the D 1000 The green Power On light on the front panel will go out i 36 g me 10 12 13 F Light Sources D 1000 piisi NO Lift up the black protective shutter and disconnect your optical fiber from the SMA connector Replace the red plastic cap over the SMA connector Bulb Replacement Order a replacement bulb item D 1000 B from Ocean Optics Make sure the D 1000 is turned off the power cord is disconnected and the lamp has cooled Use a Phillips head screwdriver to remove all 12 screws from the side panels of the D 1000 casing Do not remove any screws from the front back or bottom panels Remove the cover Find the deuterium bulb It is located at the front of the housing mounted on a black platform Three wires lead from the bottom of the bulb to the electronic board one red wire with a time indicator and two black wires Use a Phillips head screwdriver to loosen the screws securing these three wires to the green electronic board Once the screws are loose gently remove the red wire and the two black wires You do not need to completely remove the screws to detach the wires Note that on the green electronic board just right of each wire is a letter To the right of the red wire is the letter A To the right of one black wire is the letter H and to the r
121. ithout Enba mo selecting Enabled the time acquisition Spectrometer Channel Master 7 process will not calculate data Enable Plotted to see a real time graph of the Wavelength nm 400 Bandwidth pixels er acquired data in a spectral window Select a Spectrometer Channel for your Factor multiply fo Offset add fo time acquisition process Inthe Wavelength nm box specify the analysis wavelength In the Bandwidth pixels box specify the number of pixels averaged around the analysis wavelength Select a multiplicative Factor to apply to the __ Cancel _ ss e data before it is plotted or stored Select an additive constant or Offset to apply to the data after the factor is applied and before the data is plotted or stored The equation for the Factor and Offset functions is Results Factor x Data Offset 3 If desired configure a time acquisition process for the second single wavelength by selecting the Channel B page To configure a time acquisition process for the third fourth fifth and sixth single wavelengths select the Channel C Channel D Channel E and Channel F pages respectively and set the necessary parameters 4 If desired configure a time acquisition process for a combination of two time channels by selecting Combination 1 Select Enabled to set the time acquisition calculation for the wavelength Enable Plotted to see a real time graph of the acquired data in a spectral window Specify Time
122. ition of the switch Example 768 decimal Hex300 0x300 Default Setting Nalueas shown ass ot Example 784 decimal Hex310 0x310 Nauueas shown ve i o e Example 800 decimal Hex320 0x320 Vvatueas shown 16 32 256 512 switch is in the on upward position 100 CO Appendix A ee Interrupt Request Settings for the ADC1000 and PC2000 To change the IRQ settings on the ADC1000 and PC2000 see the bank of switches on the A D board The IRQ may be changed via the last 3 switches The following matrix defines the different IRQ settings by switch positions 7 8 and 9 In the default setting the IRQ is set to 7 Other combinations for IRQ settings are below After you have changed the switches reinstall the card and change the software settings to match the hardware settings See pages 19 22 for instructions The gray block indicates the position of the switch Interrupt Request 3 switch is in the on upward position a 101 ean PLCS me F Appendix B Adjusting the Focus for Collimating Lenses In order to obtain accurate data the light entering and exiting a sample by means of a fiber collimating lens assembly must be well collimated The following is a description of how to adjust the focus of light so that accurate data is collected by the spectrometer All collimating lenses are already adjusted at the time of manufacture such that light emerging from a 200 u
123. k stability allow 30 minutes warm up time before taking your measurements 66 F ean Sampling Chambers ISS UV VIS Our Operating the ISS UV VIS through Software 1 Connect the 15 pin accessory cable from the spectrometer to the ISS UV VIS 2 Find the switch on the back of the ISS UV VIS There are three positions On Off and Remote For Software operation move the switch to the Remote position Moving the switch to the Remote position enables you to control the lamp through the software whether you are using OOIChem or OOIBase32 software When using OOIChem software select Spectrometer Enable Strobe from the menu to turn it on and off 4 When you want to control the ISS UV VIS through OOIBase32 software select or deselect the Strobe Enable box in the dialog bar above the graph area to turn the bulbs on and off 5 There can be up to a 1 5 second delay between turning the bulbs on via the software and the bulbs igniting If the lamp has not been used recently the deuterium bulb may take up to 60 seconds to ignite 6 For 0 3 peak to peak stability allow 30 minutes warm up time before taking your measurements 2 Disabling the Tungsten or Deuterium Bulb It is possible to disable the deuterium or the tungsten bulb in the ISS UV VIS Both bulbs are enabled at the time of manufacture In order to disable the deuterium or tungsten bulb you must remove the casing of the ISS UV VIS Disabling the Deuterium Bulb Jumper block
124. l Manually Stopped smaller this number the more frequently data is saved The larger Initial Delay Hours Frequency Hours Duration Hours this number the less frequently data is fio Minutes fio Minutes jeo Minutes d b s l q z Seconds Seconds C Seconds saved but entering a large number C Miliseconds C Miliseconds C Miliseconds enhances the performance of the time acquisition process fo At specified time intervals data from Cancel OOIBase32 is stored into time acquisition e channels or combination channels This data can be simply plotted in a spectral window or streamed to disk or both Up to 2048 acquisitions can be displayed in a spectral window If more than 2048 acquisitions are gathered only the last 2048 of them will be displayed To store more than 2048 acquisitions you must stream the data to disk Writing data to the disk is a slow process relative to the speed of some spectral acquisitions and causes a decrease in system performance However writing data to disk more frequently gives a larger margin of safety Enable Show Values in Status Bar to see the time acquisition values in the status bar These values replace the cursor values Name the Stream Filename for the time acquisition process Clicking on the ellipsis to the right of this box opens a file save dialog box allowing you to navigate to a designated folder Enable Save Every Acquisition to store data for every spectral acquisition during
125. lame analysis of Astronomy Aerospace re entry rockets Consumer based and quality control applications in the automotive Consumer Analytical cosmetic home diagnostic coating paint and gemology industries In This Manual This manual provides users with directions on configuring your A D converter with your computer and operating the S2000 Miniature Fiber Optic Spectrometer For abbreviated directions on setting up your system turn to the Quick Start instructions beginning on page 8 In addition this manual covers instructions for using some of our most popular spectroscopic components including light sources sampling chambers sampling optics fiber optic probes and optical fiber assemblies The final section in this manual provides specific directions on taking absorbance transmission relative irradiance and reflection measurements in the Experiment Tutorial section There is a separate manual for OOIBase32 Spectrometer Operating Software fb The operating instructions for components that make up our pre configured systems such as the Raman spectroradiometry fluorescence oxygen and pH sensing and chemistry lab teaching systems are not included in this manual F Introduction PtiCSme Packing List A packing list comes with your order It is located inside a plastic bag attached to the outside of the shipment box The invoice is mailed separately The items listed on your packing slip include all of the components in yo
126. lamps to remove the fibers Adjusting the Fit of the Cuvette The CUV ALL is preset for use with a 1 cm quartz cuvette When properly adjusted the cuvette should fit snugly in the holder 1 Locate the two ball plunger screws They are under two of the four collimating lenses 2 Remove the fiber clamps for easier access to the screws 3 Use the provided screwdriver to loosen the two ball plunger screws until the ball end of the screws is just visible in the cell holder 4 Insert your cuvette into the holder Tighten the ball plunger screws until the ball contacts the cuvette and starts to compress Do not over tighten A 58 ean Sampling Chambers CUV ALL ee Installing Filters 1 Loosen the filter clamping screw with the provided screwdriver 2 Insert the filter into the filter slot The filter slot can accommodate filters up to 6 mm thick 3 Clamp the filter in place by gently tightening the clamping screw finger tight Using the Temperature Stabilization Feature This feature is used to heat or cool the cuvette holder base and cuvette 1 Remove the two plugs from the top side of the base The plug on the right side of the base should stay in place but might require thread tape 2 Replace the plugs with the two barbed fittings or any 1 8 NPT pipe thread fittings Thread tape might be required on the fittings 3 Connect the fittings to a water source Water will circulate through the base
127. le Strobe Reserved F read ROG F clock CO N OD or AJ GO PO o oO M wo A ea o1 O J2 D SUB 15 Accessory Connector I 5 Function Strobe Single Fire Strobe Multiple Fire 5 VDC External Hardware Trigger External Synchronization Trigger Analog Channel 7 Input Analog Channel 6 Input External Software Trigger Analog Channel 1 Ground Analog Channel 4 Analog Channel 5 Digital Out 0 Strobe Enable or Lamp On Off Analog Channel 3 Analog Channel 2 CO N OD or AJ V PO o oO k e N wo A ji ol 108 ean PLCS inc F Appendix F External Triggering Our S2000 and S1024DW Spectrometers when used with Configure Data Acquisition x OOIBase32 Spectrometer Operating Software provide four methods of acquiring data In the Normal Mode Ocean Optics Basic Extemal Trigger Strobe spectrometers are free running That is the spectrometer is continuously scanning acquiring and transferring data to your External Trigger Mode computer according to parameters set in the software In this Nowe z mode however there is no way to synchronize the scanning acquiring and transferring of data with an external event I Automatically save file on trigger To synchronize data acquisition with external events three oth
128. lenses on the attenuator and take out the inner barrel 2 Slip in the adapter and tighten the set screw 3 Loosen the set screw on the collimating lens of the light source Remove the inner barrel of the lens and slide the adapter attenuator into the collimating lens Tighten the set screw Specifications Dimensions 1 5 x 1 5 x 1 0 Assembly ports 3 8 24 threads for collimating lenses diameter 3 32 Adiusiment MNES material black anodized aluminum with knurled edge Adjustment wheel lock 6 32 Nylon thumb screw Connector SMA 905 79 F Sampling Optics WS 1 a WS 1 Diffuse Reflectance Standard The WS 1 DIFFUSE REFLECTANCE STANDARD is a compact physical standard for use in performing diffuse reflectance measurements especially color analysis The reflectance material that comprises the WS 1 is Spectralon a highly lambertian thermoplastic substance that provides a nearly 100 diffuse reflective surface for applications from 200 nm to 2 5 um With an Ocean Optics spectrometer the practical use of the WS 1 is limited to 200 1100 nm The reflective area of the WS 1 is 1 25 in diameter The diffusing material is encased in a small black receptacle with a screw on top Operation Reflection is the return of radiation by a surface without change in wavelength Reflection is expressed as a percentage relative to the reflection from a standard substance such as the WS 1 white reference material
129. m fiber collimating lens assembly is collimated Adjust the focus of the collimating lens on your light source i Connect the fiber that you are going to use as the illumination fiber in your setup to the light source Ensure the connection is tight The female SMA connector of the fiber must be screwed all the way into the male connector of the lamp Turn on the lamp and inspect the light beam emitted from the other end of the fiber by pointing the fiber at a light colored object such as a white piece of paper The distance is not too critical but should be at least 3 inches from the surface Loosen the set screw on the fiber barrel of the light source with an Allen wrench After the set screw has been loosened slide the inner barrel of the collimating lens on the light source until you see an even intensity across the beam spot The spot of light should be uniform there should be no fluctuations in intensity and color There should not be any dimmer rings of light surrounding the center spot Once the inner barrel is positioned so that a well focused uniform spot of light is obtained tighten the set screw with the Allen wrench Do not put down the fiber and then tighten the set screw Try to continue to hold the fiber 3 inches from the surface while you tighten so that if the inner barrel of the collimating lens slips and distorts the spot of light you will be able to readjust the focus Now the collimating lens on the light source is focused f
130. may be changed via the first 6 switches and the IRQ may be changed via the last 3 switches To first check your computer to see which settings are available follow the instructions for your Windows operating system Windows 95 and Windows 98 Users Find Available Base Address and IRQ Settings me Go to Start Settings Control Panel and double click on the System icon 2 Choose the Device Manager tab and double click on Computer at the top of the list 3 Under View Resources find available settings numbers unassigned to hardware Note these available settings for both the Interrupt request IRQ and the Input output Base Address When you first run OOIBase32 you must enter these values in the Configure Hardware dialog box Remember that Input output settings are expressed in hexadecimal Computer Properties 2x Computer Properties 2 x View Resources Reserve Resources View Resources Reserve Resources C Direct memory access DMA C Interrupt request IRQ Direct memory access DMA C Input output 1 0 C Memory Setting Hardware using the setting System timer Standard 101 102 Key or Microsoft Natural Keyboard Programmable interrupt controller Communications Port COM2 Communications Port COM1 Standard Floppy Disk Controller Hardware using the setting 0261 Motherboard resources 02FF Communications Port COM2 0376 Intel 823714B EB PCI Bus Master IDE Controller 0376 Secondary IDE controlle
131. mm 75 83 and 90 mm focal lengths are also available for some configurations SMA 905 14 different gratings 5 10 25 50 100 or 200 um slits Slits are optional In the absence of a slit the fiber acts as the entrance slit Sony ILX511 CCD 2 order rejection long pass optional 3 65 000 msec 250 1 single acquisition 3 5 counts RMS 20 counts peak to peak 0 3 10 0 nm varies by configuration lt 0 05 at 600 nm lt 0 10 at 435 nm 8 master plus up to 7 slaves 30 70 C Storage 10 60 C Operation 12 F ean S2000 Miniature Fiber Optic Spectrometers ee S2000 Board Layout Hi JP2 JP6 5 11025 J2 D SUB 15 H1 Header Block connecting analog signals from slave spectrometer channel s to the Master spectrometer channel H2 Header Block containing digital control signals JP3 Jumper Block controlling of external strobing of a light source JP4 Jumper Block controlling externally triggered integration time of the spectrometer JP5 Reserved do not short JP6 Reserved do not short RV1 Potentiometer controlling the baseline RV2 Potentiometer controlling the gain do not adjust Ji A D Converter interface cable connection J2 Accessory Cable connection 13 ean IPLICS inc First in Photonics A D Converters This section covers the basic installation instructions for our A D converters ADC500 ADC1000 and PC2000 SAD500 and DAQ700 Because A D converte
132. mode varies with scan rate 44 ean PLCS inc F Light Sources LS 450 LS 450 Blue LED Pulsed Light Source The LS 450 BLUE LED PULSED LIGHT SOURCE is a compact low cost light emitting diode that produces pulsed or continuous spectral output at 470 nm the blue region for high sensitivity emission fluorescence measurements The LS 450 excitation source can be combined with other sampling optics for fluorescence applications Operation 1 Plug the wall transformer into a standard 110V outlet and into the back of the LS 450 2 Screw an optical fiber onto the SMA connector on the front of the light source Using the Continuous Mode 1 Turn the switch on the back of the LS 450 to contin The continuous mode simply means that the light coming from the LS 450 is continuous 2 To turn the lamp off simply change the position of the switch to off Using the Pulsed Mode 1 Plug one end of the DB 15 accessory connector into the back of the LS 450 and the other end into the back of the S2000 2 Turn the switch on the back of the LS 450 to pulsed for pulsed mode of operation 3 The pulsing of the LS 450 is controlled through the spectrometer Remove your spectrometer from its housing Do not tamper with the optical bench If you have more than one channel in your system you may have to disconnect the channels from one another The master spectrometer is always on the bottom of a multiple channel s
133. mples To take an absorbance measurement using OOIBase32 our spectrometer operating software follow these steps 1 Make sure you are in scope mode by either clicking the scope mode icon on the toolbar or selecting Spectrum Scope Mode from the menu Make sure the signal is on scale The peak intensity of the reference signal should be about 3500 counts 2 Take a reference spectrum by first making sure nothing is blocking the light path going to your sample The analyte you want to measure must be absent while taking a reference spectrum Take the reference reading by clicking the store reference spectrum icon on the toolbar or selecting Spectrum Store Reference from the menu This command merely stores a reference spectrum You must select File Save Reference from the menu to permanently save the spectrum to disk Storing a reference spectrum is requisite before the software can calculate absorbance spectra 3 While still in scope mode take a dark spectrum by first completely blocking the light path going to your sample If possible do not turn off the light source If you must turn off your light source to store a dark spectrum make sure to allow enough time for the lamp to warm up before continuing your experiment Take the dark reading by clicking the store dark spectrum icon on the toolbar or selecting Spectrum Store Dark from the menu This command merely stores a dark spectrum You must select File Save Dark from the menu t
134. n you want to control the MINI D2T through OOIBase32 Spectrometer Operating Software select or deselect the Strobe Enable box in the Acquisition Parameter dialog bar above the graph area to turn the light source in the MINI D2T on and off 5 There can be up to a 1 5 second delay between turning the bulbs on via the software and the bulbs igniting If the lamp has not been used recently the deuterium bulb could take up to 60 seconds to ignite 6 For 0 3 peak to peak stability allow 30 minutes warm up time before taking your measurements Disabling the Tungsten or Deuterium Bulb It is possible to disable the deuterium or the tungsten bulb in the MINI D2T Both bulbs are enabled at the time of manufacture In order to disable the deuterium or tungsten bulb you must remove the casing of the MINI D2T Disabling the Deuterium Bulb It is possible to enable or disable the deuterium lamp Jumper block JA of the MINI D2T s circuit board controls the deuterium bulb Short pins 2 3 that is place a jumper over pins 2 3 of JA to enable the deuterium bulb Short the pins 1 2 that is place a jumper over pins 1 2 of JA to disable the deuterium bulb Disabling the Tungsten Bulb It is possible to enable or disable the tungsten lamp as well Jumper block JB of the MINI D2T s circuit board controls the tungsten bulb Short pins 2 3 that is place a jumper over pins 2 3 of JB to enable the tungsten bulb Short pins 1 2 that is place a jumper over pins
135. nds immediately to the right of the frequency entry Enter a value to set the Duration for the entire time acquisition process Be sure to select Hours Minutes Seconds or Milliseconds to the right of the duration entry 8 Click the OK button for the Time Acquisition Configuration dialog box 9 Initiate the Time Acquisition mode by either clicking on the alarm clock icon in the Time Acquisition toolbar or selecting Time Acquisition Activate Time Acquisition from the OOIBase32 menu You can then start a time acquisition process by selecting the start icon or by selecting Time Acquisition Start from the menu 97 ean lt tics Appendix A Changing the A D Converter Settings Base Address Settings for the ADC500 To change the Base Address settings on the ADC500 board see the bank of 6 dip switches labeled SW1 Switches in the OFF position have the decimal values shown Switches in the ON position have a value of zero The Base Address is the sum of the values of the switches In the default setting switches 5 and 6 are added to give a total of 768 A few of the many combinations for Base Address settings are below After you have changed the switches reinstall the card and change the software settings to match the hardware settings See pages 15 18 for instructions The gray block indicates the position of the switch Example 768 decimal Hex300 0x300 Default Setting faswaon i Example 800 decimal Hex3
136. ng lenses VIS NIR BK 7 glass 360 nm 2 um 5 mm diameter f 2 Collimating lenses UV VIS NIR Dynasil 1100 quartz 200 nm 2 um 5 mm diameter f 2 Collimating lens termination SMA 905 Collimating lenses assembly sample compartment dimensions 2 0 x 1 5 LW Filter slot accepts filters up to 1 4 6 mm in thickness Base material aluminum Base length 5 5 water inlet Connections in base water outlet extra connection Water input fittings 1 8 NPT pipe thread Typical optical fibers specified for optimum performance light throughput and optical resolution 200 um illumination fiber 50 um read fiber Though the VIS NIR lens is optimized for use to 2 um it can be configured to see only to 1100 nm with our S2000 spectrometer There is no single combination of optical fibers that will satisfy the requirements of every application As a rule however it is best to use a large diameter gt 50 um illumination fiber to get the maximum light throughput and a small diameter lt 50 um read fiber to achieve the best optical resolution 56 F Sampling Chambers CUV UV 10 CUV VIS 10 O CUV UV 10 CUV VIS 10 Cuvette Holders The CUV UV 10 and CUV VIS 10 CUVETTE HOLDERS for 10 cm cuvettes couple to our spectrometers and light sources to create spectrophotometric systems for absorbance and transmission measurements of aqueous solutions and gas
137. nnector and adding a new component or accessory Our list of spectroscopic accessories includes sampling holders in line filter holders flow cells and other sampling devices fiber optic probes and sensors an extensive line of optical fibers and accessories and collimating lenses attenuators diffuse reflectance standards and integrating spheres This modular approach components are easily mixed and matched offers remarkable applications flexibility Users pick and choose from hundreds of products to create distinctive systems for an almost endless variety of optical sensing applications Just a few of the applications requiring our systems follows Field Applications Requiring Ocean Optics Systems Noninvasive medical diagnostics such as optical biopsy blood analysis Medical and Life Sciences glucose monitoring and cancer detection Pharmaceutical processing and dissolution monitoring color calibration and Process and Quality Conirol food processing and techniques in the oil gas chemical paper polymer and textile industries Ocean monitoring hazardous site evaluation water treatment air Environmental Monitoring pollution control stack emissions ozone depletion and soil and water contamination analysis Semiconductor Technologies Plasma diagnostics end point detection and thin film thickness Research and Education Scientific research and teaching aid Emission of celestial bodies and burn efficiency and f
138. ntact us and obtain a Return Merchandise Authorization RMA number Please contact an Ocean Optics Applications Scientist for specific instructions when returning a product ean PLCS inc F Quick Start The S2000 is easy to set up allowing the user to start collecting data within minutes The three pages in this section provide brief instructions on setting up your system installing your A D converter installing and configuring the software and connecting sampling optics If you prefer step by step directions for setting up and operating any part of your system check the Table of Contents to find instructions on a specific component Step 1 Interface the A D Converter to your PC lf your A D card is the ADC500 or the ADC1000 or if you are installing a PC2000 1 The default settings for our A D products are a Base Address or Input Output Range of 768 decimal and an IRQ of 7 You will need to match Base Address and IRQ settings on the A D card to available settings in your computer First determine which settings are not being used by other hardware devices If you have Windows 95 98 go to Start Settings Control Panel Double click the System icon Choose the Device Manager tab and double click on Computer at the top of the list of devices Under View Resources note available settings numbers unassigned to hardware numbers not listed here Remember that these I O settings are expressed in hexadecimal and correspond to o
139. ntact with the metal housing There is no filter clamping screw for holding filters in place The most useful filters include the following an FG 3 blue filter for increasing the relative energy near 400 nm and 800 nm compared to 600 nm an IR cutoff filter to reduce stray light below 750 nm a550 nm long pass filter to eliminate second order effects on Shortwave NIR measurements Replacing the Bulb 1 Order a replacement bulb LS 1 B for a 900 hour bulb or LS 1 LL B for a 10 000 hour bulb 2 Turn off the LS 1 and allow the lamp to cool 3 Use an Allen wrench to loosen the set screw on the bottom of the lamp You do not need to remove the set screw This screw holds the bulb in place 41 F Light Sources LS 1 ptits 4 Locate the two set screws at the back of the lamp one above each back leg These two screws keep the two halves of the lamp together Remove the two screws 5 Gently separate the two halves of the lamp 6 Carefully pull the bulb out of its housing Detach the wire and lamp leads from the socket Remove the old bulb unit and discard 7 Plug the new bulb into the socket 8 Slide the new bulb forward into the front of the lamp as far as it will go 9 Gently tighten the set screw on the bottom of the lamp 10 Close together the two halves of the lamp being careful not to pinch the wires 11 Replace the two screws at the back of the lamp For directions on adjusting the focus of the collimating
140. nu Go through each page in the Spectrometer Configuration dialog box to set system parameters See the OOIBase32 Spectrometer Operating Software Manual for details Inthe Wavelength Calibration page the coefficients for each spectrometer channel in your system have already been loaded as part Spectrometer Configuration x Wavelength Calibration A D Interface Reference Monitoring Stray Light Correction Detector Linearity es of the spectrometer configuration file Check Pectometer Type s2o00 ec2000 gt the Enabled box for each spectrometer A D Converter Type DA0700 7 channel in your system Interupt Request RQ 7 gt Inthe A D Interface page enter the same values as you did in the Configure Hardware dialog box The Detector Linearity page in this dialog box allows you to enter coefficients for an algorthim that corrects for rare occurances of non linearity of the detector Contact Ocean Optics for more information Save the spectrometer configuration file by Cancel Apply Help choosing Spectrometer Save Configuration As from the OOIBase32 menu You can rename the file or use the default file name your serial number spec You will then be asked if you would like to make this file the default spectrometer configuration file Choose Yes The next time you run OOIBase32 the software will use the file as the standard for your spectrometer configuration When you exit OOIBase32 any changes to the config
141. o permanently save the spectrum to disk Storing a dark spectrum is requisite before the software can calculate absorbance spectra 4 Begin an absorbance measurement by first making sure the sample is in place and nothing is blocking the light going to your sample Then choose the absorbance mode icon on the toolbar or select Spectrum Absorbance Mode from the menu To save the spectrum click the save icon on the toolbar or select File Save Processed from the menu R If at any time any sampling variable changes including integration time averaging boxcar smoothing distance from light source to sample etc you must store a new reference and dark spectrum illumination computer A typical configuration for an absorbance experiment 92 First in Photonics ean Experiment Tutorial Transmission CO Transmission Experiments Transmission is the percentage of energy passing through a system relative to the amount that passes through the reference Transmission Mode is also used to show the portion of light reflected from a sample Transmission and reflection measurements require the same mathematical calculations The transmission is expressed as a percentage T relative to a standard substance such as air The software calculates T or Ra by the following equation S D T Bp xX 1009 oT 4 R D 00 where S is the sample intensity at wavelength A D is the dark intensity at wavelength A R
142. og Box First a prompt to enter a user name and serial number appears Certain data files will include this information in the header If at a later date you wish to change the operator name and serial number select Edit Settings from the menu and then choose the Registration tab Click OK Default Spectrometer Configuration File Next the following message appears This appears to be the first time OOIBase32 has been executed Please select a spectrometer configuration file from the following screen This spectrometer configuration file will be used each time OOIBase32 is started A file open dialog box then appears Navigate to the OOIBase32 directory and choose the default spectrometer configuration file the file with spec as the extension preceded by the serial number of your spectrometer A default spectrometer configuration file will be named something similar to 12J613 spec 23 ean A D Converters SAD500 CO First in Photonics Configure Hardware Dialog Box Next the Configure Hardware dialog box opens The parameters in this dialog box are usually set only once when Configure Hardware Ocean Optics Windows Device Driver 32 bit OOIBase32 is first installed and the software first runs l 2 3 Version 3 01 00 Under Spectrometer Type choose your spectrometer Te Under A D Converter Type choose SAD500 Spectrometer Type 5200087 Under Serial Port choose the COM port number your 2000 PC200
143. og Box Select Spectrometer Configure from the menu and set system parameters In the Wavelength Calibration page the coefficients for your system were loaded as part of the spec file Check the Enabled box for each channel in your system In the A D Interface page enter the same values as you did in the Configure Hardware dialog box When you exit OOIBase32 this information is stored in the spectrometer configuration file 00lBase32 Settings Dialog Box Choose Edit Settings from the menu to configure OOIBase32 parameters in the OOIBase32 Settings dialog box Go through each page of this dialog box to select options for saving opening and printing data to choose waveform sound files for various program events to configure default setting files and to select other important options such as storing and copying data and choosing warning messages 9 First in P CO Quick Start PtiCSme Configure Data Acquisition Dialog Box Finally select Spectrum Configure Data Acquisition from the menu to open the Configure Data Acquisition dialog box and to set your data acquisition parameters such as the integration time averaging and boxcar smoothing and several other parameters Step 4 Connect Sampling Optics For detailed information on connecting and operating sampling optics such as light sources sampling chambers fibers or any other Ocean Optics spectroscopic accessory check the Table of Contents to find operating instruction
144. og box accept the default or choose Browse to pick a directory Click Next gt 3 At the Backup Replaced Files dialog box select either Yes or No We recommend selecting Yes If you select Yes accept the default or choose Browse to pick a destination directory Click Next gt Select a Program Manager Group Click Next gt At the Start Installation dialog box click Next gt Follow all prompts regarding the Spectrometer Configuration diskette that came with your system At the Installation Complete dialog box choose Finish gt When prompted to do so restart your computer when the installation is complete Do not run OOIBase32 at this time Your computer must be properly configured to use the DAQ 700 before you can use OOIBase32 9o ION Install NI DAQ Windows 95 98 and NT users must install NI DAQ Driver Software the device driver library necessary for Windows 95 98 NT systems to properly use the DAQ 700 1 Insert your NI DAQ version 6 CD into your CD ROM drive 2 After you insert your CD a setup program should automatically start If it does not run the Setup exe program from the CD The installation program has an option called Install NI DAQ Select that option 4 In the Select Components dialog box make sure NI DAQ Driver Files Minimal Install is checked Choose any of the other options you wish to install Click Next gt Accept the default destination directory and the default Program Grou
145. olution testing and Raman spectroscopy You can also purchase device drivers and code for software development Custom programming services are also available For detailed instructions on all of the features menu items and options in OOIBase32 please refer to the PDF version of the OOIBase32 Spectrometer Operating Software Manual on the Ocean Optics Software and Resources Library CD Free OOIBase32 upgrades are available via our web site at www OceanOptics com 32 ean IPLICS inc First in Photonics Light Sources Ocean Optics light sources provide illumination and excitation in the UV VIS or Shortwave NIR range for absorbance reflection and fluorescence measurements discrete line spectra for wavelength calibrations and calibrated absolute intensity standards for irradiance measurements Wall transformers are supplied for laboratory use Most of the light sources are powered by 12 VDC to facilitate their use in the field Note that though some light sources emit energy lt 200 nm or emit energy to 2 um their use with Ocean Optics spectrometers is limited by our detector s responsivity 200 1100 nm The MINI D2T MINIATURE DEUTERIUM TUNGSTEN LIGHT SOURCE is optimized for the UV It produces continuous DT 1000 spectral output from 200 850 nm The D 1000 DEUTERIUM LIGHT SOURCE is a high output fiber optic light source optimized for the UV It produces intense continuous spectral output from 200 400 nm Th
146. ompletely ro Ga bo Using the Fiber Supports 1 Snap the clamps around the fibers after the fibers are screwed into the light source and spectrometer 2 Lift the clamps until they support the fibers 3 Unsnap the clamps to remove the fibers Using the Shutter Use the shutter to take dark readings The shutter is in the OPEN position when the knurled light block wheel shows a scored depression shown open on the drawing The shutter is in the CLOSED position when the light block wheel is rotated so only the knurling shows the wheel will lock into this position with a positive action SMA collimating assembly Filter Slot Light Block Filter Thumb Wheel shown without Wheel shown shown clased filter open 68 ean Sampling Chambers FHS UV FHS VIS CO Specifications BK 7 glass 360 nm 2 um FHS VIS Collimating lenses VIS NIR 5 mm diameter f 2 Dynasil 1100 quartz 200 nm 2 um FHS UV Collimating lenses UV VIS NIR 5 mm diameter f 2 Collimating lens termination SMA 905 Filter slot T ea or any size gt 1 2 square up to 1 4 Base material aluminum Though the product can be used to 2 um it can be configured to see only to 1100 nm with our S2000 spectrometer 69 F Sampling Chambers LPC Wee LPC Long Pass Flow Cells LPC Lone Pass FLOW CELLS from Ocean Optics couple to the company s high sensitivity fiber optic spectrometers and compact light
147. omputer Properties x View Resources Reserve Resources View Resources Reserve Resources C Direct memory access DMA C Interrupt request IRQ Direct memory access DMA Memory g i Memory Hardware using the setting System timer Standard 101 102 Key or Microsoft Natural Keyboard Programmable interrupt controller Communications Port COM2 Communications Port COM1 Standard Floppy Disk Controller Setting Hardware using the setting 0260 0261 Motherboard resources J 02F8 02FF Communications Port COM2 0376 0376 Intel 823714B EB PCI Bus Master IDE Controller 50376 0376 Secondary IDE controller dual fifo F 0378 037F ECP Printer Port LPT1 0380 03B8 Matrox Millennium G200 AGP English O3C0 O03DF Matrox Millennium G200 AGP English nara anre n 1 am moe ECP Printer Port LPT 1 munn 5 For most computers the default settings work well In the picture above left it appears that the Printer occupies IRQ 07 but for most computers our A D converters can share the IRQ 07 setting with a printer and conflicts will not arise All computers have multiple Base Address Input output settings from which to choose 15 ean A D Converters ADC500 CO First in Photanics BRYON me a Windows NT Users Find Available Base Address and IRQ Settings Go to Start Programs Administrative Tools Common Windows NT Diagnostics In the Windows NT Diagnostics d
148. on 3 Set other acquisition parameters in the software via the Acquisition Dialog Bar or the Configure Data Acquisition dialog box To access the dialog box click the Data Acquisition icon or select Spectrum 110 F Appendix F piisi NO Configure Data Acquisition from the menu 4 Select Spectrum Configure Data Acquisition from the menu Choose the External Trigger page and select Synchronization 5 To save processed data with each external trigger enable the Automatically save file on trigger box If you enable this function you will be presented with a file save dialog box with each trigger To avoid manually naming a file for each trigger you can enable the Autoincrement Filenames function by selecting File Autoincrement Filenames Enabled from the menu Choose a base name and starting index for the autoincremented files 6 Once you select an external trigger mode it will appear on your computer that your spectrometer is unresponsive Instead it is waiting for the trigger Activate your triggering device The acquisition parameters name of the window acquiring data and trigger mode are displayed in the main status bar External Hardware Trigger In this edge triggered mode the spectrometer is idle until you apply a trigger to the spectrometer With each trigger the following occurs 1 the spectrometer is cleared and then begins integrating 2 the spectrometer integrates for a fixed period of time determined by the
149. or the fiber Adjust the focus of the next collimating lens in your setup 5 For this part of the procedure the illumination fiber is still connected to the lamp and its collimating lens and the lamp is still on Now take the second collimating lens in your setup removed from the illumination side of a cuvette holder for example and screw it onto the other end of the illumination fiber Make sure the fiber and the second collimating lens are completely connected Point this end of the fiber at least 2 meters away from a wall If the beam spot on the wall is too faint you may need to dim the room lights Loosen the set screw that holds the barrel of this second collimating lens in place and slide the barrel until the spot of light focused on the wall has crisp edges You are looking for the sharpest and cleanest image possible Tighten the set screw Do not put the fiber down and then tighten the set screw Try to continue to hold the fiber with the spot of light focused on the wall while you tighten the set screw so that if the inner barrel of the collimating lens slips and distorts the image while tightening you will be able to readjust the focus Now that the second collimating lens has been refocused remove it from the end of the fiber and install it back into your setup back into a cuvette holder for example Continue to adjust the focus of the other collimating lenses in your setup 7 You have completed adjusting the focus of the
150. ounting collimating lenses on breadboard laboratory tables rail carriers and other bench plates Application Tips The 74 OPM Optical Post Mount has an 8 32 x 1 2 bore for mounting collimating lenses on breadboard laboratory tables rail carriers and other bench plates The 74 OPM is 1 1 2 in diameter and 0 4 thick and can be used to mount lenses securely in place in a variety of positions Mount the Optical Post Mount onto a post for your laboratory breadboard or any other bench plate Post and screw are not included Screw a collimating lens into the Optical Post Mount Specifications Assembly material black anodized aluminum Dimensions 1 1 2 diameter x 0 4 thick Threads 3 8 24 Bore for mounting 8 32 x 1 2 77 F Sampling Optics 74 ACH O 74 ACH Adjustable Collimating Lens Holder The 74 A CH ADJUSTABLE COLLIMATING LENS HOLDER is a versatile assembly for mounting lenses at multiple positions and is especially useful for transmission measurements of large or thick samples not easily accommodated by other sampling optics such as our FHS UV and FHS VIS In line Filter Holders Application Tips The 74 ACH consists of an anodized aluminum base and adjustable mount bars Each bar has four 3 8 24 threaded holes spaced 1 apart starting 1 from the top of the bar to accommodate collimating lenses The bars can be adjusted on the base by loosening two 10 32 threaded set screws with a 5 32
151. p 6 In the Ready to Install dialog box click Next gt When prompted to do so restart your computer You must restart your computer at this time g H Install the DAQ 700 1 After the computer restarts wait until all disk drive activity stops that is wait until your computer is completely restarted Connect the spectrometer cable between your DAQ 700 and your spectrometer 2 Insert the DAQ 700 into any available PCMCIA slot 3 Windows 95 98 will play a sound consisting of two tones of increasing pitch If you do not hear this sound and you do have internal speakers in your notebook computer try turning the speaker volume up and reinserting the DAQ 700 If you still do not hear the happy sound contact our Technical Support Department 4 To find IRQ and Base Address values follow the instructions for your Windows operating system 26 A D Converters DAQ 700 First in Photonics ean IPLICS inc For Windows 95 98 Users Configure the DAQ 700 If you hear the happy sound click Start and select Settings Control Panel Double click the System icon Select the Device Manager tab In the Device Manager dialog box find the hardware group named Data Acquisition Devices Either double click the group or select the group and click Properties Under the Data Acquisition Devices group find the entry for your DAQ 700 Either double click DAQCard 700 or select the entry and click Properti
152. pe 2 Replace the plugs with the two barbed fittings or any 1 8 NPT Pipe thread fittings Thread tape may be required on the fittings 3 Connect the fittings to a water source Water will circulate through the base Specifications Collimating lenses VIS NIR BK 7 glass 360 nm 2 um 5 mm diameter Collimating lenses UV VIS NIR Dynasil 1100 quartz 200 nm 2 um 5 mm diameter Base material and length Aluminum 5 5 Water input fittings 1 8 NPT pipe thread Though the VIS NIR lens is optimized for use to 2 um it can be configured to see only to 1100 nm with our S2000 spectrometer 57 F Sampling Chambers CUV ALL e CUV ALL 4 way Cuvette Holder The CUV ALL 4 way CUVETTE HOLDER for 1 cm path length cuvettes has four collimating lenses that couple to optical fibers light sources and spectrometers to measure absorbance fluorescence scattering or any combination of these optical phenomena Parts Included CUV ALL assembly for holding 1 cm cuvettes Black Cover for eliminating ambient light Two Barbed Fittings for connecting a temperature stabilizing water source Screwdriver for adjusting the fit of the cuvette Allen wrench for adjusting the collimating lenses eee eo a Operation Attaching the Fibers for Absorbance Measurements 1 For UV measurements attach one end of a SMA terminated optical fiber to one of the collimating lenses with the UV label and a red lens cap Attac
153. power supply Two Lamp Calibration Reports one for using the LS 1 CAL with a bare fiber and one for using the LS 1 CAL with a CC 3 cosine corrected irradiance probe A disk that holds files for both lamp calibration reports Allen wrench for adjusting the inner barrel of the SMA connector naih di i o Caution The LS 1 CAL becomes HOT during operation DO NOT insert flammable materials into the filter slot The materials could melt or ignite Handle with care m The LS 1 CAL is not designed as an illumination source for absorbance transmission or reflection measurements The LS 1 Tungsten Halogen Light Source should be used for those applications xa Setting Up The LS 1 CAL is designed to calibrate the absolute spectral response of your system Before beginning make sure you have your LS 1 CAL your spectrometer an SMA terminated optical fiber or CC 3 cosine corrected irradiance probe and OOIIrrad Ocean Optics Irradiance Software If you have a spectrometer setup that is highly sensitive you may not be able to use the LS 1 CAL as a calibration source Using the LS 1 CAL for Calibration Use an Allen wrench to loosen the set screw on the LS 1 CAL s SMA connector If you are using a bare fiber with the lamp withdraw the inner barrel from the SMA connector Screw this connector barrel onto the end of your fiber The connection should be tight Insert the barrel fiber all the way into the LS 1 CAL s SMA connector If you
154. r Larger fibers and slits will have lesser optical resolution Also keep in mind that if the spectrometer has no slit and your experimentation involves using optical fibers of different diameters wavelength calibration with each fiber you anticipate using will be necessary Calibration is also recommended each time you unscrew the fiber from the spectrometer 3 Find the on off switch next to the SMA connector and turn the lamp on The red indicator will light when the lamp is on Calibration You are going to be solving the following equation which shows that the relationship between pixel number and wavelength is a third order polynomial A 1 C p Cyp Cp where is the wavelength of pixel p I is the wavelength of pixel 0 C is the first coefficient nm pixel C is the second coefficient nm pixel2 and C is the third coefficient nm pixel3 You will be calculating the value for J and the three Cs 49 First in Photonics CO Light Sources HG 1 PHICS me 1 After placing OOIBase32 into Scope Mode take a spectrum of your light source Adjust the integration time until there are several peaks on the screen that are not off scale 2 Move the cursor to one of the peaks and carefully position it so that it is at the point of maximum intensity Record the pixel number that is displayed in the status bar located beneath the graph Repeat this step for all of the peaks in your spectrum 3 Using your spreadsheet
155. r dual fifo 037F ECP Printer Port LPT1 03BB Matrox Millennium G200 AGP English ECP Printer Port LPT1 nunni 03DF Matrox Millennium G200 AGP English I For most computers the default settings work well In the picture above left it appears that the Printer occupies IRQ 07 but for most computers our A D converters can share the 07 setting with a printer and conflicts will not arise All computers have multiple Base Address Input output settings from which to choose 19 First ean A D Converters ADC1000 and PC2000 Oe RUD me oe Windows NT Users Find Available Base Address and IRQ Settings Go to Start Programs Administrative Tools Common Windows NT Diagnostics In the Windows NT Diagnostics dialog box click on the Resources tab Select the IRQ button Find an available IRQ a number unassigned to a device Select the I O Port button Find an available I O Range Base Address a number or range of numbers unassigned to a device The number is in hexadecimal Note these available settings When you first run OOIBase32 you must enter these values in the Configure Hardware dialog box l Windows NT Diagnostics DAVENT Bee JE Windows NT Diagnostics WDAVENT BES File Help File Help Version System Display Drives Memoy Version System Display Drives Memory Services Resources Environment Network Services Resources Environment
156. r installation goes hand in hand with software installation you will find directions for installing OOIBase32 Spectrometer Operating Software included in this section The A D converters we offer are ADCS500 ISA Bus A D CONVERTER is a 12 bit 8 channel single ended half length card that fits into an ISA slot in a desktop PC and has 500 kHz sampling frequency ADC1000 ISA sus A D CONVERTER is a high speed 12 bit 8 channel single ended half length card that fits into an ISA slot in a desktop PC and has 1 MHz sampling frequency PC2000 PC PLUG IN is an S2000 mounted onto an ADC1000 A D card This combination fits easily into an ISA slot in the PC has 1 MHz sampling frequency SAD500 SERIAL Port INTERFACE is a 12 bit 8 channel A D converter for serial port connection for interfacing to desktop or notebook PCs and has 500 kHz sampling frequency DAQ 700 PCMCIA A D CONVERTER from National Instruments vil is a 12 bit 16 channel single ended card that fits into a credit card ADC1000 ISA bus size slot in a notebook PC and has 100 kHz sampling frequency A D Converter Our software includes drivers that support the A D converters we offer Other A D converters can be used with our spectrometers if the following conditions are met the ADC sampling rate is at least 100 kHz external triggering of the A D conversions is supported there are a sufficient number of clocks and digital output lines to operate the spectrometer a s
157. rating Software First in Photonics ean PLICS inc Other advanced features provide you with several data collection options You have the ability to independently store and retrieve dark reference sample and processed spectra All data can be saved to disk using autoincremented filenames You can save data as ASCII files or in the native GRAMS 32 SPC format One feature prints the spectra and another copies spectral or graphical data into other software such as Microsoft Excel and Word Other OOIBase32 extras include the ability to monitor the status bar for each spectral window which reflects numerous parameters set by the user manipulate the placement of an array of dockable toolbars and choose sound cues for a variety of spectroscopic events You can also designate how you receive data acquisition warnings such as when the scope mode signal has saturated in absorbance transmission and irradiance modes In addition the time normalization function allows you to designate separate integration times for reference and sample scans OOIBase32 is the latest edition of operating software for all Ocean Optics spectrometers This change from 16 bit to 32 bit operating software reflects Ocean Optics decision to upgrade its software to accommodate the increase of 32 bit operating systems OOJBase our original operating software could not work with Windows NT Additionally OOIBase did not allow for multiple channels to be shown in one
158. re IRQs each device must be assigned a unique IRQ Install the ADC500 Turn off the computer and remove the computer cover Ground yourself to the computer chassis or power supply and remove the A D card from its static shielded bag If necessary change the position of the switches on the A D board Position the switches to match the available settings you found in the previous section numbers not being used by other hardware devices See Appendix A for switch setting positions Find an open ISA bus slot and remove the slot protector Insert the A D card into an available expansion slot on the motherboard by gently rocking the card into the slot Make sure the card is fully seated in the motherboard before screwing the tab on the A D card to the computer Do not bend the card or move it from side to side once it is seated in the slot Attach the D37 end of the cable to the card and the D25 end to the spectrometer Reinstall the cover Install OOIBase32 Before installing OOIBase32 make sure that no other applications are running PWN PANN Execute Setup exe At the Welcome dialog box click Next gt At the Destination Location dialog box accept the default or choose Browse to pick a directory Click Next gt At the Backup Replaced Files dialog box select either Yes or No We recommend selecting Yes If you select Yes accept the default or choose Browse to pick a destination directory Click Next gt Selec
159. red Vis On light will come on 2 To turn off the tungsten halogen lamp press the white Visible On button located on the front panel The red Vis On light will go out Bulb Replacement Replacing the Deuterium Bulb 1 Order a deuterium replacement bulb item DT 1000 BD from Ocean Optics 2 Make sure the DT 1000 is turned off the power cord is disconnected and the source has cooled 3 Use a Phillips head screwdriver to remove all 12 screws from the side panels of the DT 1000 casing Do not remove any screws from the front back or bottom panels Remove the cover 4 Locate the deuterium bulb It is located at the front of the housing mounted on a black platform Three wires lead from the bottom of the bulb to the green electronic board one red wire and two black wires 5 Use a Phillips head screwdriver to loosen the screws securing these three wires to the green electronic board Once the screws are loose gently remove the red wire and the two black wires You do not need to completely remove the screws to detach the wires Note that on the green electronic board just to the right of each wire is a letter To the right of the red wire is the letter A To the right of one black wire is the letter H and to the right of the second black wire is the letter C 6 Use a Phillips head screwdriver to remove the two screws securing the bulb to the black platform 7 Remove the old bulb unit 8 Inspect the new bulb unit
160. researchers developed a fiber optic pH sensor as part of an instrument designed to study the role of the oceans in global warming They soon formed Ocean Optics Inc and their ingenious work earned a Small Business Innovation Research SBIR grant from the U S Department of Energy While designing the pH monitoring instrument the researchers wanted to incorporate with their sensor a spectrometer small enough to fit onto a buoy and were surprised to discover none existed So they built their own In 1992 the founders of Ocean Optics revolutionized the analytical instrumentation market filled a substantial need in the research community and changed the science of spectroscopy forever by creating a breakthrough technology a miniature fiber optic spectrometer nearly a thousand times smaller and ten times less expensive than previous systems By April 1992 just thirty days after the successful completion of Phase II of the SBIR grant Ocean Optics Inc introduced the S1000 The World s First Miniature Fiber Optic Spectrometer Due to this dramatic reduction in size and cost of optical sensing systems applications once deemed too costly or technologically impractical using conventional spectrometers were not only feasible but practical The 2000 Miniature Fiber Optic Spectrometer Our second generation miniature fiber optic spectrometer the 2000 couples a low cost high performance 2048 element linear CCD array detector with an optical bench t
161. riable changes integration time averaging smoothing angle temperature fiber size etc you must store a new dark and reference spectrum Application Tips If the signal you collect is saturating the spectrometer peaks are off the scale you can decrease the light level on scale in scope mode by Decreasing the integration time Attenuating the light going into the spectrometer Switching to a smaller diameter fiber Using a neutral density filter with the correct optical density If the signal you collect has too little light you can increase the light level on scale in scope mode by Increasing the integration time Switching to a larger diameter fiber Avoiding the use of any optical filters 91 First in Photonics ean Experiment Tutorial Absorbance CO Absorbance Experiments Absorbance spectra are a measure of how much light is absorbed by a sample For most samples absorbance is linearly related to the concentration of the substance The software calculates absorbance Aa using the following equation 2 4 A 10910 R D where Sis the sample intensity at wavelength A D is the dark intensity at wavelength A R is the reference intensity at wavelength Absorbance can also be expressed as proportional to the concentration of the substance interacting with the light known as Beer s Law Common applications include the quantification of chemical concentrations in aqueous or gaseous sa
162. roaueney Ka dPrequency Ha CW Continuous Mode 0 0 0 2 8 Divide by 2 1 5 7 6 15 2 2 Divide by 2 3 1 15 2 30 4 a Divide by 2 4 6 1 30 0 60 8 213 Divide by 2 12 2 60 8 122 0 ae Divide by 2 24 0 122 0 244 0 ait Divide by 2 48 0 244 0 488 0 2 Divide by 2 98 0 488 0 976 0 Cs Continuous Strobe N A N A Software Controlled 46 ean PLCS inc Light Sources R LS 450 Jumper Block 3 JP3 There are two sets of pins in JP3 The jumper position here determines the source of control for the R LS 450 manual or remote control A jumper over the Remote pins means that you can control the R LS 450 through the software if other jumper blocks are configured correctly R LS 450 Operating Matrix This matrix will help you configure the jumper blocks on the R LS 450 S1 Switch JP1 JP3 LED Status Off No jumper No jumper Off CW No jumper No jumper Continuously on Continuous wave mode controlled by software CW Jumpered Jumper Remote pins see Continuous Wave Mode with the 2000 s JP3 for more information CW Jumpered Jumper Manual pins Continuously on Pulsed No jumper No jumper Pulse rate determined by JP2 on the R LS 450 board see the JP2 table for pulse rates Pulsed Jumpered Jumper Remote pins Pulsed mode controlled by software see Pulsed Mode with the 2000 s JP3 for more information Pulsed Jumpered Jumper Manual pins Pulse rate determined by JP2 on the R
163. rompts before you can begin taking measurements Operator and Serial Number Dialog Box First a prompt to enter a user name and serial number appears Certain data files will include this information in the header If at a later date you wish to change the operator name and serial number select Edit Settings from the menu and then choose the Registration tab Click OK Default Spectrometer Configuration File Next the following message appears This appears to be the first time OOIBase32 has been executed Please select a default spectrometer configuration file from the following screen This spectrometer configuration file will be used each time OOlBase32 is started A file open dialog box then appears Navigate to the OOIBase32 directory and choose the default spectrometer configuration file the file with spec as the extension preceded by the serial number of your spectrometer A default spectrometer configuration file will be named something similar to 12J613 spec Configure Hardware Dialog Box Next the Configure Hardware dialog box opens The parameters in this dialog box are usually set only once when OOIBase32 is first installed and the software first opens 1 Under Spectrometer Type choose 2000 PC2000 2 Under A D Converter Type choose Suhe lenba ADC1000 PC2000 Ocean Optics Windows Device Driver 32 bit 3 Under Base Address choose the same setting as the Version 3 01 00 dip switches on the A D board
164. rough single strand optical fiber and disperses it via a fixed grating across the ae A A collection of single dual triple and quadruple channel linear CCD array detector which is responsive S2000 Spectrometers from 200 1100 um Up to seven spectrometer channels can be added to expand wavelength range perform multiple tasks or provide reference monitoring The master and slave channels are all accessed through a single program for near synchronous operation In addition an SMA 905 connector allows for easy coupling to an extensive line of fiber optic light sources sampling chambers optical fibers probes chemical sensors and other spectrophotometric accessories Ocean Optics offers nearly 500 spectrophotometric accessories that help to create fully integrated small footprint tools for optical experimentation in the lab and in the field Operating performance will vary according to a number of factors including the spectrometer configuration especially the groove density of the grating and the size of the entrance optics as well as the application itself Optical resolution measured as Full Width Half Maximum FWHM of a monochromatic source depends on the groove density lines mm of the grating and the diameter of the entrance optics optical fiber or slit The following formulas approximately An S2000 Miniature Fiber Optic Spectrometer without its housing calculate the optical resolution of your system
165. s on a specific component The following are typical configurations for absorbance transmission irradiance and reflection experiments illumination Absorbance Transmission Setup computer irradiance Irradiance Setup cosine corrector GA 6 illumination fibers computer Reflection Setup E probe holder over sample Step 5 Start the Software and Receive Data Run OOIBase32 in Scope Mode and take a dark spectrum and a reference spectrum see the Experiment Tutorial section for details Choose the absorbance transmission or relative irradiance mode to take your sample measurements For detailed information on OOIBase32 functions and features refer to the OOIBase32 Spectrometer Operating Software Manual 10 ean IPLICS inc inst in Photanics 2000 Miniature Fiber Optic Spectrometers Our second generation miniature fiber optic spectrometer the 2000 couples a low cost high performance 2048 element linear CCD array detector with an optical bench that s small enough to fit into the palm of your hand The 2000 is a high sensitivity low cost UV VIS Shortwave NIR spectrometer making it especially useful for fluorescence and other low light level applications that demand high detector sensitivity The S2000 has twice the number of CCD elements and is nearly 50x more sensitive than our original revolutionary S1000 Miniature Fiber Optic Spectrometer The S2000 accepts light energy transmitted th
166. seless for most applications The buffer materials are polymer coatings that provide mechanical strength either polyamide or Teflon Cabling further protects the buffer coated fiber Our standard laboratory cabling is blue PVC Other cabling that is available includes stainless steel monocoil The ends of the fibers are cleaved epoxied into the connectors and polished Bifurcated Assemblies and Splitters Bifurcated assemblies and splitters allow you to route light from 1 location to 2 locations or to collect light from 2 locations and combine the output into 1 location The assemblies are shaped like a Y with a stainless steel breakout located midway from the ends of the fibers The common end of bifurcated assemblies has 2 fibers laying side by side The spatial difference between the two fibers may be important in your application If this difference matters then a splitter is required The common end of a bifurcated fiber can be coupled to a larger diameter single fiber with a splice bushing to create a functional splitter 89 Optical Fiber Assemblies ean PLCS inc Splice Bushings Bulkhead Fittings Splice bushings are used to couple two SMA terminated fibers together Simply screw each fiber into the splice bushing finger tight Bulkhead fittings are used to fixture a fiber onto a panel Install the bulkhead by drilling a hole in the wall where you wish to mount the fiber Fasten with the lock washer and nut provided Sol
167. sic page allows you to set the integration time and choose averaging and boxcar smoothing values The External Trigger page allows you to specify the external trigger mode The Strobe page allows you to control external strobe events with the spectrometer See the OOIBase32 Spectrometer Operating Software Manual for details Troubleshooting Occasionally there will be problems associated with your PC configuration and the software Here are a few tips to assist you To ensure that the software and hardware are in synch exit OOIBase32 cycle power on the SAD500 and restart OOIBase372 In Windows 95 98 you can find out your Serial Port number by selecting Start Settings Control Panel Then double click on the System icon and select the Device Manager tab Double click on Ports COM amp LPT to display the COM port numbers Ensure that there is no yellow or red warning sign next to the COM Port you are attempting to use Ifthe ports on your PC are not labeled and you don t know which COM port you are using for your SAD500 you may have to resort to trial and error If you choose the wrong serial port number you will not see a dynamic trace responding to light near the bottom of the displayed graph Instead you will see a straight line Onsome computers users may have to disable any virus protection software to ensure timely and complete transfer of the data Specifications A D resolution 12 bit A
168. sing Excel look under Tools Data Analysis 5 Select the true wavelength as the dependent variable Y Select the pixel number pixel number squared and the pixel number cubed as the independent variables X After you execute the regression an output similar to the one shown below is obtained Regression Statistics Multiple R 0 999999831 R Square 0 999999663 Adjusted R Square 0 999999607 Standard Error 0 125540214 Observations 22 intercept Coefficents Standard Error Intercept 190 473993 0 36904 first coefficient X Variable 1 0 36263983 0 001684745 X Variable 2 1 174416E 05 8 35279E 07 X Variable 3 2 523787E 09 ae ees second coefficient third coefficient A 6 The numbers of importance are indicated in the above figure You will need to record the Intercept as well as the First Second and Third Coefficients Also look at the value for R squared It should be very close to 1 If it is not you have probably assigned one of your wavelengths incorrectly 7 Select Spectrometer Configure from the menu and choose the Wavelength Calibration page to update the wavelength coefficients within OOIBase32 8 Repeat this process for each channel in your setup 104 ean lt tics Appendix D S2000 Pin outs and Jumpers The average user would not normally need to know about the interconnect scheme of the S2000 as the cables supplied with all of the units need only be plugged into the matching connectors on the hardware Howev
169. sources via SMA terminations for simple efficient measurement of low volume or low concentration ppb ppt aqueous samples LPC Long Pass Flow Cells are available in 1 meter 5 meter and 10 meter path lengths for absorbance measurements The Flow Cells will function with most liquids except for perfluorinated solvents having a refractive index gt 1 30 Caution Xa Do not use perfluorinated solvents with the LPC because the amorphous fluoropolymer tubing is soluble in these chemicals xa LPC Flow Cells will function with most liquids except for perfluorinated solvents having a refractive index gt 1 30 Ensure the plumbing fittings through the front panel are tight and free of leaks Minimize the injection of bubbles into the LPC since they will cause erratic results Continuous pumping will typically flush the bubbles through the system At the end of each experimental session flush the system with solvent and then pump dry Avoid leaving fluid in the LPC for extended periods of time DO NOT exceed a fluid pressure of 45 psi v o PF Operation Using the Plumbing Connections On the front panel there are two plumbing feed through ports It does not matter which one is used for the plumbing input or the plumbing output However for experimental consistency once you have assigned which fitting will be the plumbing input and which one will be the plumbing output try not to switch the plumbing configuration The tubing goes o
170. spectral window OOIBase32 still includes the functions of the original OOIBase but numerous editing viewing and spectral processing functions have been added For those customers with Windows 3 x operating systems the 16 bit OO Base is still available The chart below outlines the significant changes from OOJBase to the new and improved OOIBase32 OOIBase OOIBase32 16 bit program 32 bit program Supports up to 8 spectrometer channels with each channel in its own spectral window Supports up to 8 spectrometer channels in each spectral window Supports single A D card Supports multiple A D cards simultaneously No overlays Up to 8 overlays for each spectral window All spectral windows must share the same acquisition parameters Each spectral window can have separate acquisition parameters Limited advanced spectral features Comprehensive advanced reference features dual beam reference stray light and linearity correction time acquisition Not customizable Customizable User friendly More user friendly No internal troubleshooting Internal troubleshooting Time series acquisition of full spectra Time acquisition of both full spectra and discrete wavelengths Na time narmalizatinn Time narmalizatian Ocean Optics also offers numerous add on software programs for applications such as color and irradiance measurements oxygen sensing pharmaceutical diss
171. t a Program Manager Group Click Next gt At the Start Installation dialog box click Next gt Follow all prompts regarding the Spectrometer Configuration diskette that came with your system At the Installation Complete dialog box choose Finish gt When prompted to do so restart your computer when the installation is complete 16 F ean A D Converters ADC500 Per Run OOIBase32 After you restart your computer navigate to the OOIBase32 icon and select it The first time you run OOIBase32 after installation you must follow several prompts before you can begin taking measurements Operator and Serial Number Dialog Box First a prompt to enter a user name and serial number appears Certain data files will include this information in the header If at a later date you wish to change the operator name and serial number select Edit Settings from the menu and then choose the Registration tab Click OK Default Spectrometer Configuration File Next the following message appears This appears to be the first time OOIBase32 has been executed Please select a default spectrometer configuration file from the following screen This spectrometer configuration file will be used each time OOlBase32 is started A file open dialog box then appears Navigate to the OOIBase32 directory and choose the default spectrometer configuration file the file with spec as the extension preceded by the serial number of your spec
172. t on the A D converter There are dip switches on the A D board and their positions determine the values These default values are set in the operating software as well Most of the time these default settings will work with your computer However if you have many devices installed in your computer you may have a conflict other devices may be using these settings If there is a conflict with another device in your computer you must change the positions of the switches on the A D board and change the values in the software For the ADC500 the Base Address is set via the bank of 6 switches labeled SW1 on the A D board and the IRQ is set via the bank of 4 switches labeled SW2 on the A D board To first check your computer to see which settings are available follow the instructions for your Windows operating system Windows 95 and Windows 98 Users Find Available Base Address and IRQ Settings me Go to Start Settings Control Panel and double click on the System icon 2 Choose the Device Manager tab and double click on Computer at the top of the list 3 Under View Resources find available settings numbers unassigned to hardware Note these available settings for both the Interrupt request IRQ and the Input output Base Address When you first run OOIBase32 you must enter these values in the Configure Hardware dialog box Remember that Input output settings are expressed in hexadecimal Computer Properties x C
173. t sources When combined with our spectrometers and light sources the CUV ALL and CUV FL DA can measure absorbance fluorescence scattering or any combination of these optical phenomena ISS ISS 2 and ISS UV VIS INTEGRATED SAMPLING SYSTEMS consist of compact light sources with integrated cuvette holders for 1 cm cuvettes These versatile sampling devices couple to our spectrometers via optical fibers to create integrated small footprint sampling systems The ISS 2 pictured is designed specially for use with the 2000 FHS UV and FHS VIS IN LINE FILTER HOLDERS provide a convenient platform for rapid absorbance or transmission measurements of optical and other filters These filter holders come with a pair of 5 mm diameter f 2 collimating lenses to maximize light throughput and a manual light block wheel for taking dark readings The filter holders accept 1 round or any size square optical filters up to 6 mm in thickness LPC Longe Pass FLOW CELLS couple to our fiber optic spectrometers and compact light sources to measure low concentration ppb ppt aqueous samples LPC Flow Cells are available in 1 meter 5 meter and 10 meter path lengths for absorbance measurements and in a 1 meter path length with built in 365 nm excitation source for fluorescence measurements The CUV CCE ELECTROPHORESIS SAMPLE CELL for chromatography and capillary electrophoresis is an optical fixture for measuring the absorbance of fluids in chromatogr
174. tails Configure Data Acquisition Dialog Box Finally select Spectrum Configure Data Acquisition from the menu to set your data acquisition parameters in the Configure Data Acquisition dialog box The Basic page allows you to set the integration time and choose averaging and boxcar smoothing values The External Trigger page allows you to specify the external trigger mode The Strobe page allows you to control external strobe events with the spectrometer See the OOIBase32 Spectrometer Operating Software Manual for details Specifications Board architecture design half length ISA bus card for single slot in desktop PC Resolution 12 bit Sampling frequency 500 kHz maximum 37 pin end to A D card 25 pin end to spectrometer IMenace carte length is 3 5 slightly gt 1 meter Multiple channel capability supports up to 8 spectrometer channels set dip switch for Base Address default 768 decimal 300 hexadecimal Installati ters AE EES set dip switch for IRQ default 7 Integration time 5 milliseconds to 60 seconds with S2000 series spectrometers 18 First in Photonics ean A D Converters ADC1000 and PC2000 CO ADC1000 ISA bus A D Converter and PC 2000 The ADC1000 ISA Bus A D CONVERTER is a 12 bit 8 channel single ended A D card that connects our spectrometers to desktop PCs The PC2000 PC PLUG IN SPECTROMETER has our 2048 element linear CCD array fiber optic spec
175. te you wish to change the operator name and serial number select Edit Settings from the menu and then choose the Registration tab Click OK Default Spectrometer Configuration File Next the following message appears This appears to be the first time OOIBase32 has been executed Please select a spectrometer configuration file from the following screen This spectrometer configuration file will be used each time OOIBase32 is started A file open dialog box then appears Navigate to the OOIBase32 directory and choose the default spectrometer configuration file the file with spec as the extension preceded by the serial number of your spectrometer A default spectrometer configuration file will be named something similar to 12J613 spec Configure Hardware Dialog Box Next the Configure Hardware dialog box opens The parameters in this dialog box are usually set only once when OOIBase32 is first installed and the software first opens 1 Under Spectrometer Type choose your spectrometer 2 Under A D Converter Type choose DAQ700 3 If you are a Windows 95 98 user choose the same Base Ocean Optics Windows Device Driver 32 bit Address value selected in steps 8 and 9 on page 28 If you are a Windows NT user choose the same Base Address Version 3 01 00 value selected in step 4 in the section on page 28 Secs 20008T S2000 PC2000 Ert The I O Range Base Address you selected was expressed in hexadecimal In this di
176. the clamps to remove the fibers Installing Filters 1 Loosen the filter clamping screw with the provided screwdriver 2 Insert the filter into the filter slot The filter slot can accommodate filters up to 6 mm thick 3 Clamp the filter in place by gently tightening the clamping screw finger tight Adjusting the Fit of the Cuvette The Cuvette Holder is designed to hold 1 cm square cuvettes When properly adjusted the cuvette should fit snugly into the holder 1 Locate the two ball plunger screws 2 Remove the fiber clamps for easier access to the screws 3 Use the provided screwdriver to loosen the two ball plunger screws until the ball end of the screws is just visible in the cell holder and insert your cuvette into the holder 4 Tighten the ball plunger screws until the ball contacts the cuvette and starts to compress Do not over tighten Using the Temperature Stabilization Feature This feature is used to heat or cool the cuvette holder base and cuvette 1 Remove the plugs from the top side of the base The plug on the right side of the base should stay in place but may require thread tape 2 Replace the plugs with the two barbed fittings or any 1 8 NPT pipe thread fittings Thread tape may be required on the fittings 3 Connect the fittings to a water source Water will circulate through the base 55 Sampling Chambers CUV UV CUV VIS ean ptics Inc Specifications Path length 1 cm Collimati
177. the dark reading by clicking the store dark spectrum icon on the toolbar or selecting Spectrum Store Dark from the menu This command merely stores a dark spectrum You must use the Save Dark command to permanently save the spectrum to disk Storing a dark spectrum is requisite before the software can calculate transmission spectra 4 Begin a transmission measurement by first making sure the sample is in place and nothing is blocking the light going to your sample Then choose the transmission mode icon on the toolbar or select Spectrum Transmission Mode from the menu To save the spectrum click the save icon on the toolbar or select File Save Processed from the menu f5 If at any time any sampling variable changes including integration time averaging boxcar smoothing distance from light source to sample etc you must store a new reference and dark spectrum illumination computer A typical configuration for a transmission experiment 93 First in Photonics ean Experiment Tutorial Reflection CO Reflection Experiments Reflection is the return of radiation by a surface without a change in wavelength The reflection may be Specular in which the angle of incidence is equal to the angle of reflection Diffuse in which the angle of incidence is not equal to the angle of reflection Every surface returns both specular and diffuse reflections Some surfaces may return mostly specular reflection
178. then select the channel you are calibrating A dialog box opens with the message Verify lamp was ON for at least 15 minutes for a REFERENCE scan Make sure nothing is blocking the light path going to your spectrometer Your setup spectrometer fiber and sampling optics for taking a reference should be identical to your setup for measuring your sample Taking a reference spectrum is requisite before the software can calculate absolute irradiance measurements Click OK Another dialog box opens with the message Block light path to spectrometer for a DARK scan If possible do not turn off the light source Instead completely block the light path going to your spectrometer by inserting an opaque object into the lamp s filter slot Taking a dark spectrum is requisite before the software can calculate absolute irradiance measurements Click OK The spectral response of your system is calibrated If you selected Display calibration info when calculating in the Configure Spectrometer dialog box you will now see the results of the calibration procedure The top left graph represents the linear 15t order polynomial regression The top right graph represents the dark scan The bottom graph is the calibration curve representing the spectral response of the process The calibration procedure is saved in a file named CH0 CAL for calibrating the master spectrometer channel CH1 CAL for calibrating the first slave spectrometer channel and so on Specifications
179. trometer A default spectrometer configuration file will be named something similar to 12J613 spec Configure Hardware Dialog Box Next the Configure Hardware dialog box opens The parameters in this dialog box are usually set only once when OOIBase32 is first installed and the software first opens i 1 Under Spectrometer Type choose your spectrometer 2 Under A D Converter Type choose Ocean Optics Windows Device Driver 32 bit ADC500 PC 1000 Version 3 01 00 3 Under Base Address choose the same setting as the dip switches on the A D board and the same available liaalbinacll Da Lies s2000 First setting you found in your computer eee Pixel 4 D Converter Type jo Remember that the Input output Range Base Address ADCS00 PC1 000 x you selected was expressed in hexadecimal In this dialog box the base address is given in decimal Base Address 1 0 Range IRQ Interrupt Request followed by the hexadecimal equivalent in parenthesis 768 040300 7 7 For example 768 0x0300 gives the base address Serial Port Baud Aate as 768 decimal and 300 hexadecimal fi 7 115200 7 S4D500 Pixel Resolution 4 Under IRQ choose the same setting as the dip A Compress SAD500 Data switches on the A D board and the same available setting you found in your computer 5 For your setup only these parameters apply Ignore the other settings they apply to other A D converters Click OK You can always change these sett
180. trometer channels number of supportable channels is Multiple channel capability function of electrical current limitations 30 First in Photonics ean PLICS inc OOIBase32 Spectrometer Operating Software OOIBASE32 SPECTROMETER OPERATING SOFTWARE is our next generation of operating software for all Ocean Optics spectrometers OOIBase32 is a 32 bit user customizable advanced acquisition and display program that provides a real time interface to a variety of signal processing functions for Windows 95 98 and Windows NT users With OOIBase32 you have the ability to perform spectroscopic measurements such as absorbance reflectance and emission control all system parameters collect data from up to 8 spectrometer channels simultaneously and display the results in a single spectral window and perform reference monitoring and time acquisition experiments OOIBase32 allows you to perform the three basic spectroscopic experiments absorbance reflectance and emission as well as signal processing functions such as signal averaging boxcar pixel smoothing electrical dark signal correction and stray light correction Scope mode the OOIBase32 operating mode in which raw data signal is displayed allows you to establish these signal conditioning parameters The basic concept for the software is that real time display of data allows you to evaluate the effectiveness of your experimental setup and data processing selections m
181. trometer mounted onto an ADC1000 This sturdy combination fits easily into a slot in the PC The following are directions for installing your ADC1000 and PC2000 Because A D converter installation goes hand in hand with software installation you will find directions for installing OOIBase32 Spectrometer Operating Software included in this section as well Each device in or connected to your computer is assigned specific settings it s similar to giving each device its own name so that your computer will know what to call and how to recognize the device In order for your ADC1000 or PC2000 to work as a device in your computer it has to be assigned a Base Address setting and an IRQ setting The default settings for each are Base Address I O Range 768 decimal 300 hexadecimal IRQ Interrupt Request 07 These default values are set on the A D converter There are dip switches on the A D board and their positions determine the values These default values are set in the operating software as well Most of the time these default settings will work with your computer However if you have many devices installed in your computer you may have a conflict other devices may be using these settings If there is a conflict with another device in your computer you must change the positions of the switches on the A D board and change the values in the software For the ADC1000 and PC2000 there is only one bank of switches on the A D board the Base Address
182. trometer to your computer The 2000 can interface to a desktop PC via our ADC500 and ADC1000 ISA bus A D cards We also offer the SAD500 Serial Port Interface which works with either a desktop or notebook PC The S2000 can also interface to a notebook PC via National Instruments DAQCard 700 PCMCIA A D card First in Photonics CO Introduction PHICS me OOIBase32 Spectrometer Operating Software is our next generation of operating software for all Ocean Optics spectrometers OOIBase32 is a 32 bit user customizable advanced acquisition and display program that provides a real time interface to a variety of signal processing functions for Windows 95 98 Windows 2000 and Windows NT users With OOIBase32 you have the ability to perform spectroscopic measurements such as absorbance reflectance and emission control all system parameters collect data from up to 8 spectrometer channels simultaneously in real time display the results in a single spectral window and perform reference monitoring and time acquisition experiments f For those customers with Windows 3 x operating systems Ocean Optics has 16 bit spectrometer operating software available the original OOIBase Contact Ocean Optics for more information Ocean Optics offers a complete line of light sources for UV VIS Shortwave NIR applications All light sources have SMA 905 terminations for coupling to optical fibers Changing the sampling system is as easy as unscrewing a co
183. trum The Transmission Mode uses the same formula for transmission and reflection measurements Diffuse Reflectance Measurements For a diffuse reflection measurement attach the illumination leg of your reflection probe to a light source and the read leg to the spectrometer Place the end of the probe in the 45 aperture of the RPH 1 Use the cap screws on the holder to secure the probe at the desired distance from the sample 1 First take a reference spectrum Make sure nothing is blocking the light path going to your reference Place the reflection probe probe holder over a diffuse standard Take the reference reading 2 Next take a dark spectrum Completely block the light path going to your sample Do not turn off the light source Take the dark reading 3 Finally take your reflection measurement Make sure the sample is in place and nothing is blocking the light going to your sample If using OOIBase32 click on the Transmission icon to take your spectrum The Transmission Mode uses the same formula for transmission and reflection measurements 85 ean PLCS inc F Fiber Optic Probes and Accessories T300 T300 RT UV VIS Transmission Dip Probe The T300 RT UV VIS TRANSMISSION DIP PROBE couples to our spectrometers and light sources to create small footprint optical sensing systems for measuring in situ transmission in chemical solutions and other liquids The standard T300 RT UV VIS Transmission Dip Probe has 2 300 um
184. trum of an RF coupled deuterium UV light source and a tungsten halogen VIS Shortwave NIR light source in a single optical path The combined spectrum light source produces a peak to peak stability of 0 3 from 200 850 nm Parts Included MINI D2T Miniature Deuterium Tungsten Light Source Power cord for connecting the MINI D2T to outlet 15 pin accessory cable for software control of the MINI D2T Caution kA The beam emerging from the MINI D2T produces ultraviolet radiation Direct eye contact could cause eye injury Safety eyewear is recommended Never look directly into the light source or stare at the diffuse reflected beam Dangerous voltages present NO serviceable parts inside unit To replace bulbs contact Ocean Optics This instrument should not be used for any clinical or diagnostic purposes BUT ye Handle with care Dropping the instrument may cause permanent damage Operation Setting Up the MINI D2T 1 Attach an optical fiber the illumination fiber to the SMA connector on the front of the MINI D2T and attach the other end of this fiber to the SMA connector on your sampling chamber Connect the second optical fiber the read fiber from the sampling chamber to the spectrometer If you have a direct attach cuvette holder on the MINI D2T simply attach an optical fiber from the cuvette holder to the spectrometer 2 Plug the wall transformer into a standard 110 V outlet Plug the 12 V output into the back of your MIN
185. tte The ISS UV VIS is designed to hold 1 cm square cuvettes When properly adjusted the cuvette should fit snugly into the holder 1 Locate the two ball plunger screws 2 Use the screwdriver to loosen the ball plunger screws until the ball end of the screw is just visible in the holder 3 Insert your cuvette into the holder 4 Gently tighten the screws until the ball contacts the cuvette and starts to compress Do not over tighten Setting Up the ISS UV VIS 1 Attach an optical fiber to the SMA connector on the front of the ISS UV VIS and attach the other end of this fiber to the SMA connector on your spectrometer 2 Plug the wall transformer into a standard 110 V outlet Plug the 12 V output into the back of your ISS UV VIS above the 12V label For users of European version wall transformers plug the transformer into a standard 220 V outlet At this time the green LED indicator light on the front of the lamp will be lit This indicator light only means that the lamp is receiving power not that the deuterium and tungsten bulbs are on Operating the ISS UV VIS Manually 1 Find the switch on the back of the ISS UV VIS 2 There are three positions On Off and Remote For Manual operation move the switch to the On position There can be up to a 1 5 second delay between switching the lamp to on and the bulbs igniting If the lamp has not been used recently the deuterium bulb may take up to 60 seconds to ignite 3 For 0 3 peak to pea
186. tware via the Acquisition Dialog Bar or the Configure Data Acquisition dialog box To access the dialog box click the Data Acquisition icon or select Spectrum Configure Data Acquisition from the menu 4 Select Spectrum Configure Data Acquisition from the menu Choose the External Trigger page and select Software Trigger 5 To save processed data with each external trigger enable the Automatically save file on trigger box If you enable this function you will be presented with a file save dialog box with each trigger To avoid manually naming a file for each trigger you can enable the Autoincrement Filenames function by selecting File Autoincrement Filenames Enabled from the menu Choose a base name and starting index for the autoincremented files 6 Once you select an external trigger mode it will appear on your computer that your spectrometer is un responsive Instead it is waiting for the trigger Activate your triggering device The acquisition parameters the name of the window acquiring data and the trigger mode are all displayed in the main status bar It is important to note that since this is a level triggered mode the amount of delay between the trigger pulse and when a spectrum is acquired is indeterminate because the delay is dependent upon how fast the software polls the Pin 8 line and recognizes it is HIGH the amount of time until the start of the next integration period External Synchronization Trigger In th
187. ty is also a result of the direct attachment of the CUV FL DA to the light source The CUV FL DA has a 74 UV collimating lens to collect the light and funnel it to the fiber Collection efficiency spatial resolution transmission angle and acceptance or collection angle are adjustable and are easily optimized The CUV FL DA is packaged with the collimating lens screwed on at a 90 angle from the light source for fluorescence measurements However the lens is removable and can be relocated for straight through absorbance measurements Operation Using with the D 1000 and DT 1000 To attach the CUV FL DA to the D 1000 or the DT 1000 the safety shutter must first be removed 1 Use a Phillips head screwdriver to remove all 12 screws from the side panels of the D 1000 or DT 1000 casing Do not remove any screws from the front back or bottom panels Remove the cover 2 Remove the screw securing the safety shutter in place 3 The inner barrel of the collimating lens for the lamp must also be removed Using the Allen wrench remove the small silver screw on the inner barrel of the lens and pull out the inner barrel 4 Place the CUV FL DA over the SMA termination and secure the holder to the lamp by gently tightening the set screw on the CUV FL DA Adjusting the Fit of the Cuvette The CUV FL DA is preset for use with a 1 cm cuvette When properly adjusted the cuvette should fit snugly in the holder 1 Check that the mirrored screws are
188. uitable software driver has been written a suitable cable has been fabricated SA lie ae Other A D converters and software can be used to control our products however you will need to create your own drivers Contact one of our Applications Scientists for timing diagrams pinouts and other necessary engineering details PC2000 Spectrometer S2000 mounted onto an ADC1000 14 First in Photonics ean A D Converters ADC500 CO ADC500 ISA bus A D Converter The ADC500 ISA Bus A D CONVERTER is a 12 bit 8 channel SA bus analog to digital converter card that connects our spectrometers to desktop PCs This single ended half length card fits into an ISA slot in a desktop PC and has a 500 kHz sampling frequency The following are directions for installing your ADC500 Because A D converter installation goes hand in hand with software installation you will find directions for installing OOIBase32 Spectrometer Operating Software included in this section as well Each device in or connected to your computer is assigned specific settings it s similar to giving each device its own name so that your computer will know what to call and how to recognize the device In order for your ADC500 to work as a device in your computer it has to be assigned a Base Address setting and an IRQ setting The default settings for each are Base Address I O Range 768 decimal 300 hexadecimal IRQ Interrupt Request 07 These default values are se
189. um ME probe holder over sample A typical configuration for a reflection experiment 94 First in Photonics ean Experiment Tutorial Relative Irradiance CO Relative Irradiance Experiments Irradiance is the amount of energy at each wavelength from a radiant sample In relative terms it is the fraction of energy from the sample compared to the energy collected from a lamp with a blackbody energy distribution normalized to 1 at the energy maximum Relative irradiance is calculated by the following equation gt where Bis the relative energy of the reference calculated from the color temperature at wavelength A S is the sample intensity at wavelength A D is the dark intensity at wavelength A R is the reference intensity at wavelength A Common applications include characterizing the light output of LEDs incandescent lamps and other radiant energy sources such as sunlight Also included in relative irradiance measurements is fluorescence in which case the spectrometer measures the energy given off by materials that have been excited by light at a shorter wavelength To take a relative irradiance measurement using OOIBase32 our spectrometer operating software follow these steps 1 Make sure you are in scope mode by either clicking the scope mode icon on the toolbar or selecting Spectrum Scope Mode from the menu Make sure the signal is on scale The peak intensity of the reference signal should be about 350
190. ur Base Address which is given in decimal followed by the hexadecimal equivalent in parenthesis If you have Windows NT go to Start Programs Administrative Tools Common Windows NT Diagnostics Click on the Resources tab Select the IRQ button Find an available IRQ Select the I O Port button Find an available I O Range Base Address If you have Windows 2000 go to Start Programs Administrative Tools Common Windows NT Diagnostics Click on the Resources tab Select the IRQ button Find an available IRQ Select the I O Port button Find an available I O Range Base Address 2 Note these values as you will have to configure the switches on the A D board to match these values Also when you first run OOIBase32 you must enter these values in the Configure Hardware dialog box 3 Turn off the computer and take off the computer cover Ground yourself to the computer chassis or power supply and remove the A D card from its static shielded bag 4 If necessary change the position of the switches on the A D board For the ADC500 the Base Address is set via the bank of 6 switches labeled SW1 on the A D board and the IRQ is set via the bank of 4 switches labeled SW2 on the A D board For the ADC1000 and PC2000 there is only one bank of switches on the A D board the Base Address may be changed via the first 6 switches and the IRQ may be changed via the last 3 switches See Appendix A for switch positions 5 Insert the A D
191. ur order However some items on your packing list are actually items you have specified to be installed into your spectrometer such as the grating detector collection lens and slit The packing list also includes important information such as the shipping and billing addresses as well as components on Back Order What you won t find on the packing list is OOIBase32 the free operating software that comes with every spectrometer order Wavelength Calibration Data Sheet and File _ In your shipment box you will find your spectrometer in a silver gray anti static bag Also inside this bag is a Wavelength Calibration Data Sheet wrapped around a floppy diskette This calibration sheet is unique to your spectrometer The floppy diskette contains a spectrometer configuration file that has the same data as the Wavelength Calibration Data Sheet Both the Wavelength Calibration Data Sheet and the diskette need to be retained When you install OOIBase32 Spectrometer Operating Software you will be prompted to insert this diskette into your computer That way the data from the spectrometer configuration file is installed with the software However if the diskette is ever lost you can use the Wavelength Calibration Data Sheet to enter system specific data into OOIBase32 fields Upgrades Customers sometimes find that they need Ocean Optics to make a change or an upgrade to their system In order for Ocean Optics to make these changes the customer must first co
192. uration file will be automatically saved to the default file Base Address I O Range payne X 00lBase32 Settings Dialog Box At this point it is a good idea to configure several OOIBase32 operation parameters Choose Edit Settings from the menu to open the OOIBase32 Settings dialog box Go through each page of this dialog box to select options for saving opening and printing data to choose waveform sound files for various program events to configure default setting files and to select other important options such as storing and copying data and choosing warning messages See the OOIBase32 Spectrometer Operating Software Manual for details Configure Data Acquisition Dialog Box Finally select Spectrum Configure Data Acquisition from the menu to set your data acquisition parameters in the Configure Data Acquisition dialog box The Basic page allows you to set the integration time and choose averaging and boxcar smoothing values The External Trigger page allows you to specify the external trigger mode The Strobe page allows you to control external strobe events with the spectrometer See the OOIBase32 Spectrometer Operating Software Manual for details Specifications Type PCMCIA Type II Resolution 12 bit Sampling frequency 100 kHz maximum Channels 16 channel single ended 8 channel differential Interface cable 50 pin connector to A D card 25 pin connector to spectrometer ee a supports up to 4 spec
193. urce tungsten halogen with 12 volt DC adapter Application Tips The ISP REF has two primary functions 1 to provide even surface illumination for reflectance measurements such as determining the color of flat surfaces and 2 to collect light and funnel it to an optical fiber for emission experiments such as measuring the spectral properties of an LED The ISP REF is small and compact it s just 2 11 x 2 25 x 3 25 LWH and weighs less than 1 pound yet is durable enough for use outside the laboratory All instrument electronics including the lamp which can be replaced by simply removing two screws are mounted into the bottom section of the unit The sphere is made from Spectralon a white diffusing material that provides a highly lambertian reflecting surface A simple switch allows users to manipulate the sampling optic for the inclusion J or exclusion E of specular reflectance The reflectivity value obtained by calculating the difference between the inclusion and exclusion of specular reflection is a direct measurement of the gloss of the surface Operation 1 Locate the on off switch on the front of the lamp The 1 position is the on position The 0 position is the off position Turn the lamp on 2 Locate the shutter switch It is located on the back of the sphere The T for includes position means that the resulting reflection measurement includes both specular and diffuse reflections The
194. ust be a multiple of time must be a multiple of 2 6 default 512 128 64 2 4 128 32 16 a 32 8 4 210 a S N A N A with a minimum value of 24 ms The pulses per second of the PX 2 or the repetition rate is controlled via Jumper Block JP3 on the circuit board of the S2000 It is also dependent upon the frequency of your A D card The table below shows the rep rate for the various combinations of hardware and jumper settings Note that the default setting from the factory is 216 JP3 Post Function ADC500 SAD500 ADC1000 DAQ700 1 Not enabled Not enabled Not enabled Not enabled 2 Divide by 2 Too fast Too fast 98 0 3 Divide by 2 122 0 Too fast 24 0 4 Divide by 2 30 0 60 0 6 1 5 Divide by 2 7 6 15 2 1 5 Specifications Spectral range 220 750 nm Approximate dimensions 14 cm x 10 5 cm x 4 cm LWH 5 5 x 4 1 x 1 5 LWH Power input 1 3 A 11V 220 Hz 100 mA 12V 10Hz Trigger input external TTL positive pulse via 15 pin connector Output 45 milliioules per pulse maximum 9 9 watts average power 220 Hz pulse rate maximum Pulse duration 5 microseconds at 1 3 height of pulse Lifetime 10 pulses estimated 230 days continuous operation at 50 Hz pulse rate Aperture 3mm Connector SMA 905 Timing signals available from S2000 spectrometers Multiple mode up to 220 Hz varies with A D sampling frequency Single
195. ver the plumbing fittings which are standard 4 28 threads The tubing should fit snugly over the fittings and be free of leaks 1 Attach one end of the tubing to your pump The pump used must not pump the solution so fast that the fluid pressure exceeds 45psi Remember to always turn off the pump in between taking a reference and taking sample measurements 2 Make sure you have a proper waste receptacle for the other end of the tubing The plumbing connections inside the LPC are standard industry fittings No maintenance should be required However if leaks develop the plumbing connections will need to be tightened To tighten the connections simply follow these steps 1 Remove the back panel 2 Carefully slide off the top cover being careful not to damage or pinch the tubing or fiber 3 Hand tighten the fittings and reassemble the cover and back panel Installing Fibers On the front panel there are two fiber feed through ports It does not matter which one is used for the fiber input or the fiber output However for experimental consistency once you have assigned which fiber port will be the input and which one will be the output try not to switch the fiber configuration The fiber inside the LPC has a core diameter of 200 um External coupling fibers should be 200 um or larger for maximum coupling efficiency 1 Attach one end of an illumination fiber to a port on the LPC and the other end to your light source 2 Attach one end o
196. ystem 4 In the center of the green circuit board near the optical bench find Jumper Block 3 labeled JP3 Jumper Block 3 consists of 10 pins The pins are labeled by rows 16 14 12 10 and 2 See the following chart for which pins must be jumpered according to how many pulses per second you need for the A D converter you have interfaced to your S2000 The default setting from the factory is 16 For example if you have an ADC1000 A D converter you have four choices for how many pulses per second can come out of your LS 450 976 244 60 and 15 If you select 244 pulses per second place a jumper over the pins next to the 14 label DAQ700 ADC500 SAD500 ADC1000 2000 JP3 Post Frequency Hz Frequency Hz Frequency Hz 16 98 0 488 0 976 0 14 24 0 122 0 244 0 12 6 1 30 0 60 0 10 1 5 7 6 15 2 5 When using the pulsed flash mode the user needs to ensure that a constant number of flashes occurs for every integration cycle To achieve a constant number of flashes per integration cycle the integration time must be a multiple of that shown in the table below Integration times are set in the software In OOIBase32 set the integration time in the acquisition dialog bar above the graph area See the OOIBase32 Operating Software Manual for more information about the integration time Integration time for Integration time for Integration time for 2000 JP3 setting DAQ700 must b

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